Metabolomics of urinary tract infection: a multiplatform approach Tiziana Pacchiarotta ISBN: 978-94-6182-433-2 This thesis has been partially funded by the Stichting Tropische Geneeskunde – Leiden. Printing: OffPage, Amsterdam Copyright © 2014 by T. Pacchiarotta. All rights are reserved. No part of this book may be reproduced, stored in a retrieval system or transmitted in any form and by any means, without prior permission of the author. Cover Page The handle http://hdl.handle.net/1887/25785 holds various files of this Leiden University dissertation Author: Pacchiarotta, Tiziana Title: Metabolomics of urinary tract infection : a multiplatform approach Issue Date: 2014-05-20 Metabolomics of urinary tract infection: a multiplatform approach PROEFSCHRIFT ter verkrijging van de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker, volgens besluit van het College voor Promoties te verdedigen op dinsdag 20 mei 2014, klokke 13.45 uur Tiziana Pacchiarotta geboren te Avezzano, Italy in 1981 PROMOTIECOMMISSIE Promotores: Prof.dr. A.M. Deelder Prof.dr. J.T. van Dissel Copromotor: Dr. M. Mayboroda Overige leden: Dr. W. Dunn, University of Birmingham, UK Prof.dr. R.P.H. Bisschoff, Rijksuniversiteit Groningen, Groningen Prof.dr. J.W. de Fijter Dr. L.G. Visser Prof.dr. Ir. J.A.P. Willems van Dijk Table of contents Introduction 7 Chapter 1 17 Metabolomics investigation of human infections Chapter 2 29 H-NMR-based metabolic profiling of urinary tract infection: combining multiple 1 statistical models and clinical data Chapter 3 45 Fibrinogen alpha chain O-glycopeptides as possible markers of urinary tract infection Chapter 4 61 Evaluation of GC-APCI/MS and GC-FID as a complementary platform Chapter 5 77 On-line spectral library for gas chromatography/atmospheric pressure chemical ionization-time of flight mass spectrometry Chapter 6 95 Exploratory analysis of urinary tract infection using a novel GC-APCI-MS platform General discussion and conclusion109 Appendices Summary116 Nederlandse samenvatting119 Acknowledgments 122 Curriculum vitae124 List of publications125