Cover Page The handle http://hdl.handle.net/1887/29812 holds various files of this Leiden University dissertation. Author: Saiman, Mohd Zuwairi Title: Terpenoids and terpenoid indole alkaloids in Catharanthus roseus cell suspension cultures Issue Date: 2014-11-20 Terpenoids and terpenoid indole alkaloids in Catharanthus roseus cell suspension cultures Mohd Zuwairi bin Saiman Mohd Zuwairi bin Saiman Terpenoids and terpenoid indole alkaloids in Catharanthus roseus cell suspension cultures PhD Thesis, Leiden University, The Netherlands Printed by Ipskamp Drukkers B.V., Amsterdam, The Netherlands ISBN: 978-94-6259-380-0 Cover design by Claudia Marcela Gonzaleza Arevalo (cmgonzalezarevalo.wix.com/layout) Terpenoids and terpenoid indole alkaloids in Catharanthus roseus cell suspension cultures PROEFSCHRIFT ter verkrijging van de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker, volgens besluit van het College voor Promoties te verdedigen op donderdag 20 november 2014 klokke 11.15 uur door Mohd Zuwairi bin Saiman geboren te Malakka (Maleisië) in 1983 Promotiecommissie Promotor: Prof. Dr. R. Verpoorte Co-promotores: Dr. N.R. Mustafa Dr. A.E. Schulte Overige leden: Prof. Dr. K.-M. Oksman-Caldentey (VTT Technical Research Centre of Finland) Prof. Dr. C.J. ten Cate Prof. Dr. J. Memelink Prof. Dr. P.G.L. Klinkhamer To my mother who sacrifices and my wife who compromises Contents Page Chapter 1 General introduction Chapter 2 Analysis of metabolites in the terpenoid pathway of 1 31 Catharanthus roseus cell suspensions Chapter 3 Metabolic alterations and distribution of five-carbon terpenoid 57 precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures Chapter 4 Effect of mevalonic acid feeding on the terpenoid pathway in 87 Catharanthus roseus cell suspension cultures Chapter 5 Effect of geraniol feeding and jasmonic acid elicitation on 107 terpenoid indole alkaloids accumulation in Catharanthus roseus cell suspension cultures Chapter 6 Catharanthus roseus cell suspensions overexpressing geraniol 135 synthase in the plastid and cytosol Chapter 7 Summary 167 Samenvatting 173 Acknowledgements 179 Curriculum vitae 180 Publications 181