Monika Langner, PhD email: [email protected] tel. (+4861) 65 50 265 Department of Biotechnology Cereal Biochemistry and Technology Team Specialization: genetics and plant breeding chemistry and technology of cereals Research profile • Study of variation in composition of amount and quality in store proteins of cereals (wheat, triticale, rye and oat) using electrophoretic, chromatographic and molecular methods. • Application of rheological methods to assess flour properties at the early stage of selection of wheat and triticale breeding materials. • Study of functional markers of genes determining variation in technological characteristics of grain in wheat (Ttriticum aestivum L.). • Investigation of the effect of genotype x environment interactions on variation in quantitative composition of storage proteins in cereals (wheat, triticale). • Analysis of variability qualitative traits in introgresive lines of wheat and triticale with fragments of wild species of Triticum, Aegilops and Agropyron genom. Methods • electrophoretic – slab gel electrophoresis (SDS-PAGE and A-PAGE), high performance capillary electrophoresis (zone electrophoresis, gel electrophoresis, capillary isoelectric focusing, affinity, micellar electrokinetic electrophoresis), • chromatographic - high (ultra) performance liquid chromatography (reverse-phase, size exlussion, ion exchange), • molecular – PCR amplification – gene identification by means of molecular markers, • biochemical - estimation of gluten and gluten index, determination of falling number, analysis of viscosity and amylolytic properties of cereals (Micro Visco-Amylo-Graph), • rheological – analysis of dough and gluten extensibility (TA.XT plus Texture Analyser, Dough Inflation System), analysis of dough mixing (mixograph, farinograph), baking in the oven test lab. Current research activities • Analysis of qualitative traits variability in introgressive lines of hexaploid triticale with fragments of the T. monococcum, T. aestivum, Aegilops and Agropyron genomes. • The influence of selection on frequency change of high molecular glutenin subunits encoded by Glu-1 loci in wheat populations. • Assessment of wheat quality characteristics variability based on the analysis of qualitative and quantitative composition of selected classes of proteins and rheological studies. Selected papers • Salmanowicz B.P., Langner M., Kubicka-Matusiewicz H. (2014). Variation of high molecular weight secalin subunit composition in rye (Secale cereale L.) inbred lines. Journal of Agricultural and Food Chemistry, 62: 10535−10541. • Salmanowicz B.P., Langner M., Franaszek S. (2014) . Charge-based characterisation of high-molecular-weight glutenin subunits from common wheat by capillary isoelectric focusing. Talanta 129: 9-14. • Salmanowicz B.P., Langner M., Wiśniewska H., Apolinarska B., Kwiatek M., Błaszczyk L. (2013). Molecular, physicochemical and rheological characteristics of introgressive Triticale/Triticum monococcum ssp. monococcum lines with wheat 1D/1A chromosome substitution. Int. J. Mol. Sci. 14 (8): 15595-15614. • Franaszek S., Langner M., Salmanowicz B.P. (2013). Low molecular weight glutenin proteins and their effect on the baking quality of wheat. Biuletyn IHAR 269: 3-13. • Langner M., Salmanowicz B.P. (2011). Gluten proteins and their effect on the breadmaking quality of wheat. Postępy Nauk Rolniczych nr 4/2011: 93-106. • DYLEWICZ M., SALMANOWICZ B. P., NOWAK J. (2009). Allelic variation of high molecular weight glutenin subunits in cultivated einkorn (Triticum monococcum L. subsp. monococcum). W: Genetyka i genomika w doskonaleniu roślin uprawnych. Red. Naganowska B., Kachlicki P., Krajewski P. Rozprawy i Monografie nr 18: 185-194, IGR PAN. • DYLEWICZ M., SALMANOWICZ B. P. (2009). Identification of puroindoline alleles in Polish wheat cultivars by molecular markers. Biuletyn IHAR 253: 93-101. • SALMANOWICZ B. P., DYLEWICZ M., NOWAK J. (2009). Effect of composition of high molecular weight glutenin proteins on selected rheological properties of wheat dough. W: Genetyka i genomika w doskonaleniu roślin uprawnych. Red. Naganowska B., Kachlicki P., Krajewski P. Rozprawy i Monografie nr 18: 173-184, IGR PAN. • NOWAK J., SALMANOWICZ B. P., DYLEWICZ M., BOCIANOWSKI J. (2009). Evaluation of prolamins polymorphism in varieties of Triticale (x Triticosecale Wittm.). W: Genetyka i genomika w doskonaleniu roślin uprawnych. Red. Naganowska B., Kachlicki P., Krajewski P. Rozprawy i Monografie nr 18: 195-206, IGR PAN. • SALMANOWICZ B. P., DYLEWICZ M. (2007). Identification and characterization of high molecular weight glutenin genes in Polish triticale cultivars by PCR-based DNA markers. J. Appl. Genet. 48: 347-357. Grants • NCBiR Project no: PBS2/B8/10/2013 Project title: BIOTRIGEN “Developing and implementing a model for accelerating breeding of wheat (Triticum aestivum L.) using biotechnological methods” Project co-ordinator: T. Adamski Main contractor: Monika Langner Duration: 1 October 2013 – 30 September 2016 Project no: 0592/R/P01/2007/03 Project title: „Development of methods of early selection of wheat using grain hardness evaluation and molecular and biochemical markers” Head: prof. dr hab. Tadeusz Adamski Duration:: 25 June 2007 – 31 December 2010 • MNISW Project no: N N312 329439 Project title: : „Characteristic of physical-chemical and rheological properties of grain from primitive wheat species" Project co-ordinator: Z. Kędzior Main contractor: Monika Langner Duration: 21 November 2010 – 31 December 2014 Project no: N N310435138 Project title: „Study of susceptibility to leaf rust (Puccinia triticina) and variability of qualitative traits in triticale introgresive lines with introgresive fragments of Triticum monococcum (2x) and hexaploid wheat genome” Project co-ordinator: H. Wiśniewska Main contractor: Monika Langner Duration: 17 May 2010 – 16 May 2013 • MRiRW Project no: nr HOR hn-801-8/14, poz. 1 Project title: „Research on the effectiveness of functional markers in conjunction with the analysis of rheological in microscale for the assessment of the quality characteristics of wheat”. Project co-ordinator: B. Salmanowicz Main contractor: Monika Langner Duration: 01 styczeń 2014 – 31 grudzień 2020 Project no: HOR hn - 801-19/13 poz. nr 4 Project title: „Assessment of variability in the quality characteristics of wheat based on the analysis of the composition of the qualitatively-quantitative selected classes of proteins and rheological studies” Project co-ordinator: B. Salmanowicz Main contractor: Monika Langner Duration: 01 stycznia 2011 – 31 grudnia 2013 Project no: HOR hn- 4040 dec -26/09 poz.7 Project title:„Assessment of quality of triticale in the early stages of selective breeding for the purpose grain bread and with improved feed value.” Project co-ordinator: B. Salmanowicz Main contractor: Monika Langner Duration: 1 January 2007 – 31 December 2007 Project no: HOR hn- 4040 dec -26/09 poz.4 Project title: „Assessment of wheat quality characteristics based on the analysis of storage proteins compound and rheological studies” Project co-ordinator: B. Salmanowicz Main contractor: Monika Langner Duration: 1 January 2007 – 31 December 2010