FEES REGISTRATION FORM CATEGORY FEE (RM) IBS 1,100.00 UPM 1,300.00 NAME: OUTSIDE UPM 1,500.00 INSTITUTION: Fees can be paid by Cash / Money Order/Postal Or- Workshop on “Basic Cell Culture for Cancer Research” der/Cheque to ‘Bendahari Universiti Putra Malaysia’. ADDRESS: Please present the Cash/ Money Order / Postal Order / Cheque to the secretariat on registration day. REGISTRATION & ENQUIRY CONTACT NO.: EMAIL: DESIGNATION: WORKSHOP FEE : MODE OF PAYMENT (Cash/ Money Order/Postal Order/Cheque / Vote no:__________________) Kindly return the completed registration form to: WORKSHOP SECRETARIAT, UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience Universiti Putra Malaysia Contact person:Mrs. Nooraini Mohd Ain (+603-8947 2139) Mrs. Noor Haizi Mohammad Ruslan (+603-8947 2124) Fax. No. : +603-8947 2218 Email : [email protected], [email protected] D a te : 8 – 1 0 t h A P R I L 2 0 1 4 V e n u e : I n s t i tu te o f B i o s c i e n c e , U n i v e r s i ti P u tr a M a l a y s i a Organized by: UPM-MAKNA Cancer Research Laboratory Institute of Bioscience Universiti Putra Malaysia * Theory & Practical TARGET GROUP OVERVIEW OF THE WORKSHOP Cell culture has become one of the major tools used in Life Sciences today. Cell culture provides a good model system for studying basic cell biology and biochemistry, interactions between disease – causing agents and cells and the effects of drugs on cells. Since both normal cells and cancer cells can be grown in culture, the basic differences between them can also be closely studied. Cultured cancer cells also serve as a test system to determine suitable drugs and methods for cancer treatment. Cell based assays have become increase important for the pharmaceutical industry, not only just for cytotoxicity testing but also for high throughput screening of compounds that may have potential use as drugs. In this workshop, we will introduce, demonstrate and provide theoretical concepts of human cell culture and their applications to clinical and biological research. Common methods and equipment used in laboratories will be presented and participants will have an opportunity to work with mammalian cells.  Scientists, Research Officers, Science Officers and students. (This workshop is designed for those new to mammalian cell culture or needing a refresher course on the basics. ) *TOPICS THAT WILL COVER Lectures: 1. SPEAKER 1. Prof. Dr. Rozita Rosli,PhD (Medical Genetics) 2. Dr. Norshariza Nordin,PhD (Molecular Medicine & StemCell) 3. Dr. Ahmad Faizal Abdull Razis,PhD (Molecular Toxicology) 4. Dr. Chan Soon Choy,PhD (Human Genetics) Overview of cell culture techniques in cancer research 2. Good Cell Culture Practice 3. BCCT 1: Cell line vs Primary Cell Culture 4. BCCT 2 : Managing Cell Culture 5. BCCT 3: Cytotoxicity Assay (MTT assay) Practical Sessions : 1. BCCT 1 2. BCCT 2 - Cell Thawing and cell freezing - Cell line maintenance and subculture 3. BCCT 3 - Cell Counting by haemocytometer & cell scepter 4. BCCT 4 - Determination of mycoplasma contamination 5. MTT assay 1 - Cell Plating 6. MTT assay 2 - Drug Treatment 7. MTT assay 3 - Read absorbance by using ELISA Reader ORGANIZING COMMITTEE Advisor : Prof. Dr. Abdul Rahman Omar Chairperson : Prof. Dr. Rozita Rosli Secretary : Mrs. Nooraini Mohd Ain Treasurer : Mrs. Noor Haizi Mohammad Ruslan Committee Members: Mrs. Tommini Salleh Mr. Muhammad Najib Hamzah Mrs. Norlela Ahmad Ms. Nurul’Ain Abu Bakar