LINKAGEOFTHEBARLEYEARLINESSGENEeakAND ITSPLEIOTROPICEFFECTSUNDERDIFFERENT GROWTHCONDITIONS S h o z oYASUDA INTRODUCT10N Ba r l e yc u l t i v a rK i n a i5h a sn e v e rb e e ngrownc o m m e r c i a l l yi nJ a p a n , andi t so r i g i no b s c u r e . Neve 討h e l 回s , t h i sv a r i e t ya t t r a c t e d回 伊 氾i a la t 恰n - t i o no fsomeo fb ar 1 e yb r e e d e r sand g e n e t i c i s t si n] a p a n . S o meo ft h e 回 s o n sa r et h a tK i n a i5h a sane x t r e m ee a r 1 yh e a d i n gt i m ewhengrown r i nwarmerr e g i o n so fJ a p a n ,and i sh i g h l ys e n s i t i v et ot e m p e r a t u r ea s f i r s tp o i n t e do u tbyEnomoto( 1 9 2 9 ) . T a k a h a s h iandYasuda( 1 9 6 0 )h a v e d e m o n s t r a t e dt h a tt h i ss e n s i t i v i t yt ot e m p e r a t u r es h o u l dr a t h e rb ee x 阿 倍s e da si n s e n s i t i v i t yt od a y l e n g t h .F u r t h e r m o r e ,a n o t h e ri n t e r 回 t i n g andc o n s p i c u o u sc h a r a c t e r i s t i cp e c u l i a rt oK i n a i5i st h a tt h es e e d l i n g s t I yr e s p o n d i n gt oe x p o s u r et oac o n d i t i o no fs h c i r t d a y t u r ny e l l o wpromp a tl o wt e m p e r a t u r ed u r i n gl i g h tp e r i o danda th i g ht e m p e r a t u r ed u r i n g d a r kp e r i o d . 1 ns h a r pc o n t r a s tt ot h eg r e e np l a n t so f somev a r i e t i e s ,t h ed i s c o l o r e dp l a n t so fK i n a i5were grownu n d e rt h esamec o n d i t i o n s f o u n dt ob ev e r yl o wi nc h l o r o p h y l lc o n t e n t( Y a s u d ae ta ! .1 9 6 5 ) . Thep r e s e n ts t u d ywasmadet oknowwhatk i n d so ft h eg e n ec o n t r o l l e dt h er e s p o n s e sp e c u l i a rt oK i n a i5 . 1 twasc o n f i r m e dfrom t h e 白 u l t st h a tt h er e s p o n 舘 si nK i n a i5wereg o v e r n e dbyt h ee a r l i n e s sg e n e r e p o r t e d回 r l ybyYasudaandShimoyama( 1 9 6 4 ) . Thed e t a i 1 so ft h e e a k r r e s u l t swi 1 1b ep r e s e n t e db e l o w . MATERIALSANDMETHODS Themainc h a r a c t e r i s t i 回 o fm a t e r i a l su s e df o rt h ev a r i e t yt 田t sand a sp a r e n t si nc r o s s i n ga r el i s t e di nT a b l e1 . 1 n v e s t i g a t i o n sweremadeont h ef o l l o w . i n gc h a r a c t e r s . 政 a d i n gt i m ei n af i e ! d: The p 1 a n t s were sown i naf i e l di n midi 1 yr 回o r d sweret a k e nonas i n g l ep l a n t Novembera tK u r a s h i k i,andda m e r g e n c e . b a s i sf o rd a t eo f回 re H e a d i n gt i m eu n d e rs h o r t P h o t o p e r i o d i cc o n d i t i o n s: The p l a n t s were l 1 u m i n a t i o na t18-20oC,andda i 1 yr e ∞rdswere grown u n d e r1 2 h o u ri t a k e nonas i n g l ep l a n tb a s i sf o rd a t eo ff l . a g l 回 fe m e r g e n c e. I fw i n t e r ,t h es p r o u t e ds e e d so ft h e v a r i e t i e s were i n v o l v e di nt h em a t e r i a l s s u b j e c t e dt ol o w t e m p e r a t u r e( 3 . . . . . . 50C )f o r4 0d a y so rmo 児 m a t e r i a l swere, p r i o rt op l a n t i n g . Y e ! ! o w i n gr e s p o n s et os p e c i a lg r o w t hc o n d i t i o n s : The s e e d l i n g sw e r e 1 6 S .Y a s u d a TABLE 1 C h a r a c t e r i t i c so fv a r i e t i e su記 di nt h ep r e s e n ts t u d y Nameo fv a r i e t y K i n a i5 Ka g o s h i m aGol d e n m e l o n M a r i K i n d o k u M i n n .伺 5 B t r dt e s t e r I w a t eM e n s u r yC M u y O j i R u s s i a n82 O r a n g elemma Go s e s h i k o k uSai1 N i g r i n u d u m Co l s e s s1 M a t u r i t y C h i e fc h a r a c t e r i s t i c s V e r ye a r 1 y V e r ye a r l y r 1 y Veryea Veryea r 1 y La t e La t e V e r yl a t e M i d s e a s o n L a t e La t e La t e - s 国 関n M i d M i d s e a s o n T w o r o w e d Tw o r o w e d T w o r o w e d Tw o r o w e d Tw o r o w e d .b l a c kl e m m a .sm ∞t hawn S i x r o w e d .b l a c kl e m m a .b r a c t e a t e S i x r o w e d .s h o r th a i r e dr a c h i 1 1 a S i x r o w e d .l e g u l e l e s s S i x r o w e d .a l b i n olemma S i x r o w e d .o r a n g elemma S i x r o w e d .u z uo rs e m i b r a c h y t i c .g l o s s yl e a f T w o r o w e d .n a k e dk e m e l .b l a c klemma S i x r o w e d .h o 吋e dh e a d s u b j e c t e ds e v e r a lt i m e st o8--12hour o fp h o t o p e r i o da tlQo Co rlower 0 during t h el i g h tp e r i o d and 20C o rh i g h e rduringt h edark p e r i o d . Undert h e s ec o n d i t i o n s some p l a n t sc o n s p i c u o u s l y turny e l l o w . These growthc o n d i t i o n sw i l lbed e s i g n a t e dbelowa st h es p e c i a lgrowthc o n d i t i o n sf o ry e l l o w i n g . M e a s u r e m e n to fc h l o r o P h y l lc o n t e n t : Chlorophyllo fsomev a r i e t i 回 w as e x t r a c t e dbyt h ec o n v e n t i o n a l method with a c e t o n efroml e a fsamples ( 0 . S 1 g )c o l l e c t e d from t h e same p o s i t i o n on t h e main s t e m . The s o l v e n to ft h ea c e t o n ee x t r a c t swast h e nr e p l a c e dbye t h e r .andt h ee t h e r e x t r a c t swerea n a l y z e di naBeckmanq u a r t zs p e c t r o p h o t o m e t e r , andt h e t e n twase s t i m a t e dbyK o s k i ' sformula( 1 9 5 0 ) . c h l o r o p h y l l∞n RESULTS ( 1 ) Ge n ee a "f o r1 ゐr yl ! . a r l y助 a d . n gu n d e rN a t u r a lGro ωt hC o n d i t i o n s nC h r o m o s o m e5 a n dI t sL i n k a g ew i t hB o Thevery e a r l y genee a "was established inthe following experie e d sfromtwoc r o 回目 b etweenveryearlyc u l t i v a r swith m e n t s . TheFzs whitelemma.K i n a i5andKagoshimaGoldenmelonandl a t eonewithb l a c k ,weresown togetherwith t h e i rp a r e n t si nt h ef i e l d lemma,Minn.90-5 i nmid-Novembero f1 9 6 6 . F i g .1showst h ef r e q u e n c yd i s t r i b u t i o n so f l a n t sandt h e i rp a r e n t si nd a t eo fh e a d i n g . t h eFzp ,t h ee a r l yandl a t et y p ep l a n t si nt h eFzp o p u l a Ase v i d e n ti nF i g .1 i l yc l a s s i f i e d , andt h eheadingp e r i o do ft h ee a r l yFzp l a n t s t i o n回 nbe回 s c o i n c i d e sc l o s e l ywitht h a to fK i n a i5o rKagoshimaGoldenmelononone handandt h a to ft h el a t eF2 p l a n t swithMinn.90-5ont h eo t h e r . Table 2showst h a tt h es e g r e g a t i o no fl a t eande a r l yt y p e si nt h eF2 p o p u l a t i o n 1 7 L l nkageandP l e i o t r o p l cE f f e c t so ft h eBa r 1 eyEa r l l n e s sGenee a l < F2K i n a i5XM i n n .90 ・ 5 401 n:Black lemma 図 W h i t el e m m a 内 品 回 。 ﹄ZESZ g a -, 30 hEE nvnU ・ 。 F2 K a g o s h i m aG o l d e n m e l o nXM i n n .90 ・ 5 Z Z M w -色 刷 。 ﹄ @ ﹄E22 A p r i l May F i g .1 .D a t eo fh e a d i n go fF .p l a n t sfromtwoc r o s s e so fK i n a i5and Kagoshima Goldemelon ( w h i t e lemma) w i t h Minn. 釦 5( b l a c k t s , sowno u t d ∞rsonthe15thofNovember. lemmalando ft h e i rp a r e n TABLE 2 Linkagebetw 田 ne a ,. forearlyheadingandB forblacklemmainF2 o ftwoc r o s s e so fKinai5 andKagoshimaGo ldenmelonwith Minn.9(}-5 grownunderf a l l s o w i n gc o n d i t i o n s M i n n .件 5 c r 田 s e dw i t h K i n a i5 I t em E a r 1 y La t e T o t a l 疋 B l a c k White B l a c kWhit χ2 p 2 1 3 1 1 43 4 r t L o 9 A 由 . 3 b 吋 : a g 3 . E n u I 1 Rm 田』郷 r 2 1 7 . 0 1 1 7 4 . 9 4剖 回. 3 1 回. 3 1 1 7 6 . 2 7 ( 9 . 4 4 3 1 1 . 2 0 6 . 5 6 お. 6 9 2 1 . 回 3 1 1 . 0 0 3 .4 2 .5. 3 6 . 6 9 5 ∞ G Z : : ニ出国 k a G g o o l g d M e n I n m a e l o n 3 0 5 2 0 5 7 7 . 1 9 1 171.5657.19 5 9 . 0 6 3 0 5 . 0 01 2 6 .6 6 く. 0 1 2 0 7 . 5 42 1 . 2 1 2 1 . 2 1 5 5 . 0 4 3 0 5 . 0 0 0 . 3 2 ) . 9 5 . . . . . . . . . . . . ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ー ・ ・ 噌 闘 ー ・ ー . . . . . . . . . . . . . _ . . ー . . . . . . . . . . . ー ・ ・ ・ ー ・ 噛 ・ ・ ・ ・ ・ ・ ・ ・ , ・・ 噛 ・ ・ ・ ・・ . . . . , , ・ ・ ・・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ー ・ ・ ・ ・ ・ ー ー ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・・ ・ ・ . . . . Weighteda v e r a g ev a l u eo fr e c o m b i n a t i o n(船: 1 6 . 7 8 土1.6 7 9 4 a H4 e occurred in a 3 :1ratio,indicating thatthe recessivegen回 involvedin g偲 himaGoldenmelon were res 卯 n sibleforthe very bothKinai5 andKa ear 1 y heading. Furthermore ,asapparentinFig. 1,the majorityofthe 1 8 S .Y a s u d a e a r l yt y p ep l a n t sw e r ec h a r a c t e r i z e dbyw h i t elemmaandl a t eo n e s by ,i n d i c a t i n gac l o s ea s s o c i a t i o nb e t w e e nh e a d i n gt i m e and b l a c klemma lemmac o l o r . Ther e c o m b i n a t i o nv a l u e sb e t w e e nt h eg e n e sf o rv e r y回r1 y o rb l a c kv e r s u sw h i t elemmaweree s t i m a t e d v e r s u sl a t eh e a d i n gandBbf . 5 1 7% and1 5 . 0 4土2 . 2 5 4% f o rt h ec r o s 毘 so fK i n a i5and t ob e1 8 . 9 6土2 , r e s p e c t i v e l y( T a b l e2 ) , sugg 田t KagoshimaG o l d e n m e l o nw i t hM i n n .9 0 5 白 b e t w e e nt h e s etwo白 t i ・ i n gt h a tt h e r ewere n os u b s t a n t i a ld i f f e r e n c m a t e s . Thew e i g h t e da v e r a g ev a l u eo fr e c o m b i n a t i o n加 tw 田 nt h ee a r l y o rb l a c klemmawas1 6 . 7 8土1.6 7 9% . r e c e s s i v eg e n eandB f I np a r a l l e lw i t ht h i se x p e r i m e n t ,ana l l e l i s mt e s twasmadeu s i n gF2 h y b r i d sb e t w e e nK i n a i5andKa g o s h i m aG o l d e n m e l o nandt h e i rp a r e n t a l 町 v a r i e t i e swhichweres i m u l t a n e o u s l ysowni nt h ef i e l di nmid-Novemt o f1 9 6 7 . Themodeo fv a r i a t i o nf o rh e a d i n gt i m ec o i n c i d e dc l o s e l yi n goshima G o l d e n m e l o n and t h e i r F2 h y b r i d s,andt h emean K i n a i5,Ka d a y st oh e a d i n gi nt h e s et h r e ep o p u l a t i o n sw e r ea l m o s tt h esame( T a b l e l d e n m e l o n卯 s s 白 S 3 ) .T h i si m p l i e st h a tb o t hK i n a i5andKagoshimaGo t h e回 merece罰 i v e伺 r l i n 白 sg e n e . TABLE 3 D a y sωheadingo fF2 p l a n t so fK i n a i5xK a g o s h i m aGol d e n m e l o nc r o s s 訂 e n t s田 wno u 凶 ∞r si nf a l l a n dt h e i rp 4 O一 口一 eM 一郷 町 胤 1F 司0 0 ・o K m一土 土 土 討 一7 2 3 4 ・ ・ ・・ ・ 国 一 ' a- 4 4 60 60 2 : 19 吐 一回 目 白 N o .o fp l a n t s M一 P o p u l a t i o n K i n a i5 Ka g 田 h i m aG o l d e n m e l o n F .h y b r i dp l a n t s Yasuda and Shimoyama ( 1 9 6 4 )r e p o r t e dont h er e c e s s i v ee a r l i n e s s a i n v o l v e d i n K i n a i 5 , t h o u g h t h e y c o u l d n o t e s t a b l i s h i t s l i n k a g e g e n ee k g r o u p . Ther e c e 弱 i v ee a r l i n e s sg e n ee s t a b l i s h e di nt h ep r e s e n ts t u d yc o r ι,s ot h a tt h esameg e n es y m b o li su s e di nt h i sr e p o r t . r e s p o n d st ot h ee ( 2 ) E . f f e c t s01ea~ o nE a r l i n e s su n d e rS h o r t t h o t o t e r i o d i cC o n d i t i o n I no r d e rt oi n v e s t i g a t ed i r e ct 1 yw h e t h e rt h eg e n ee 仇 e x e r t si n f lu e n c e , at o t a lo f7 0p l a n t s onh e a d i n gt i m eu n d e rs h o r t p h o t o p e r i o d i cc o n d i t i o n o p u l a t i o no fK i n a i5xM i n n . weret a k e nfromt h ea b o v e m e n t i o n e d F2 p 回 r . oft h e s e ,20p l a n t sw e r e 9 0 5grown i nt h ef i e l dt h ep r e v i o u sy l a n t sandt h er e m a i n i n g5 0p l a n t sfroml a t eF2 p l a n t s . fromt h ee a r l yF2 p 回 d i n gb e h a v i o rwast e s t e d under1 2 h o u ri l l u m i n a t i o na t1 80C T h e i rh l 1 yv e r n a l i z a t i o n . D i s c r i m i n a t i o n加t w e e ne a r l yandl a t et y 開S a f t e rf u wasq u i t ee a s y,b e c a u s et h ee ar 1 yh y b r i dp l a n t sandt h ee a r l yp a r e n t , andt h e K i n a i5e x p a n d e dt h e i rf l a g l e a v e sw i t h i n4 0d a y sa f t e rp l a n t i n g l a t et y p ep l a n t sandt h el a t ep a r e n tM i n n .9 0 5showede x p a n s i o na t6 0 LinkageandP l e i o t r o p i cE f f 回 t so ft h eBarleyEa r l i n e s sGenee a / c 1 9 d a y so rmorea f t e rp 1 a n t i n g . The2 0s t r a i n sd e r i v e dfromt h ee a r l yF z ,w h e r e a st h e5 0s t r a i n s p 1 a n t swerew h o l l yhomozygousf o re a r 1 yh e a d i n g l a n t swered i v i d e di n t otwog r o u p s :3 4h e t e r o z y g o u s fromt h el a t eF2 p ,whichgaveg∞df i t s t r a i n s and1 6s t r a i n shomozygousf o r1 a t eh e a d i n g =0 . 0 4 4 ,p>0 . 8 ) .T h i ss u g g e s t st h a tt h eg e n ee ι , is t o2 :1r a t i oχ (. z 白 p o n s i b 1 ef o rt h ee a r 1 yh e a d i n gu n d e rt h ec o n d i t i o no fs h o 吋ーp h o t o p e r i o d r andh i g ht e m p e r a t u r e . 白 i si np a r twereo b t a i n 吋 O t h e re v i d e n c e ss u p p o r t i n gt h i sh y p o t h fromt h r e ee x p e r i m e n t s .twoo fwhicha r ec r o 路e so fKagoshimaG o l d e n me10n w i t hM i n n .9 0 5 and B t r dt e s t e r . Thet h i r de x p e r i m e n twas n d u c e dfromBo n u s madeu s i n gac r o s so fane a r l ym a t u r i n gmutantMari,i ta l .1 9 6 0 )w i t hB t r dt e s t e r .I nt h e s ec r o s b o t hM i n n . ( G u s t a f s s o ne ,1 a t e rm a t u r i n g 9 0 5andB t r dt e s t e ra r ec h a r a c t e r i z e dbyb 1 a c k1emma , t h 白e andw i n t e rgrowthh a b i t .A f t e rv e r n a l i z a t i o na tl o w t e m p e r a t u r e F2 h y b r i d sandt h e i rp a r e n t sw e r esowni nf l a t sandgrown underc o n 加u t2 0oC . F i g .2showst h eh e a d t r o l l e dc o n d i t i o n so f1 2 h o u rd a ya ta 1 a n t so fa Kagoshima G o 1 d e n m e 1 0 nxM i n n .9 0 5 i n gb e h a v i o ro f F2 p l a n t sc a nb e c r o s sand t h e i rp a r e n t s . The r e s u 1 t sshow t h a tt h eF2 p 回 s i 1 yc l a s s i f i e di n t oe i t h e rt h ee a r 1 yo rl a t et y p eandt h a tt h ef l a g l e a f ぽ o fb o t ht y p 白 c o i n c i d ec l o 記 1 yt ot h ee a r l yand1 a t ep a r e n t s . emergen 田r v e dnumberso fe a r 1 yandl a t et y p 回 w ere9 5and2 9 4 ,r 回 開c t i Theo b v e 1 y ,i nt h e Kagoshima G 0 1 d e n m e l o nxM i n n .9 0 5c r o 田 , 7 9and2 2 1, 、 旬 40 口 B l a c kl e m m a 図 W h i t el e m m a nHVAHV 内ふ内, m H E w -色 刷 。 ・M@﹄自 n z L 1 0 Da y st of 1a l l l e a fe m e r l l e n c e F i g .2 . Frequencyd i s t r i b u t i o n histogramshowingdayst of 1 a g l 回 f emergenceo fF . hybrid p l a n t sandt h e i rp a r e n t si nac r o s sb e tween Kagoshima Goldenmelon ( w h i t e lemma) and Minn.9 0 5 ( b l a c klemma).grownunder 1 2 h o u rdayc o n d i t i o ni nagrowth chamber. 20 S .Y a s u d a r e s p e c t i v e 1 y ,i nt h eKagoshimaGo1denme1onxB t r dc r o 路 a nd52and1 7 6 , r e s p e c t i v e 1 y .i nt h eMarixB t r dt e s t e rcrωs ,a l lo fwhichc 1 ωe1yf i ta r e g a t i o nr a t i o . Mor 切 v e r ,alinkageo ft h ee a r l ygenew i t ht h e 1:3田 g genef o rw h i t e1emma wass u g g e s t e d byt h ef a c tt h a tt h em a j o r i t yo f t h ee a r l yp 1 a n t sa r ec h a r a c t e r i z e dbyw h i t e1emmaandt h el a t e oneby b l a c k 1emma ( F i g .2 ) . The l i n k a g ei n t e n s i t i e s betweent h er e c e s s i v e o rb 1 a c k1emmaa r e1 5 . 8 5土1. 3 9 8% and1 8 . 8 7土2 . 6 0 8% e a r 1 ygeneandBf i nt h ec r o s s e so f Ka goshima Go1denme1onw i t h Minn. 9 0 5andB t r d t e s t e r ,r e s 伊c t i v e l y ,and 16.57土2.739% i nac r o s so fMariw i t hB t r d ) . S i n c et h er e c o m b i n a t i o nv a 1 u e si nt h ec r o 部e swith t e s t e r( T a b 1 e4 TABLE 4 L i n k a g eb e t W I 民 nt h eg e n ef o re a r l yh e a d i n gu n d e rs h o r t d a y∞n d i t i o n s o rb l a c klemmai nF2o ft h et h r e ec r 田 記so fKag 田h i m a a n dB f Go l d e n m e l o na n dM a r iw i t hM i n n .9 0 5a n dB t r dt e s t e r C r o s s I t e m L a 旬W h i t eB l a c E k a r W l y h i t e T o t a l B l a c k χ 2 P Ka g o s h i m aG.r O b s v d .n u m b e r 2 6 3 3 1 2 6 6 9 3 8 9 4 5 . 4 0 3 <. 0 1 x { 9 :3 :3 :1 2 1 8 . 8 1 7 2 . 9 4 7 2 . 9 4 2 4 . 3 1 3 8 9 .∞1 M i n n .9 0 5l L i n k a g e1 5 . 8 5 %2 6 3 . 3 6 2 8 . 3 9 2 8 .3 9 回.86 3 8 9 .0 0 0 . 4 4 2 .9 ! h9 O b s v d . n u m b e r 1 9 1 2 0 3 0 4 9 2 9 0 K a g o s h i m aG.r x < 9 :3 :3 :1 1 6 3 .1 2 5 4 . 3 8 5 4 . 3 8 1 8 .1 2 2 9 0 . 0 0 9 0 . 0 1 5 <. 0 1 n .9(}-5 l Li n k a g e1 8 . 8 7 % 1 9 2 . 6 8 2 4 . 8 2 2 4 . 8 2 4 7 . 6 8 2 9 0 . 0 0 2 . 0 6 8 . 9 5 . 9 M i n J 1 O 9 u : b z z 3 凪 v 2 a 酔 3 0 n u 1 1 6 m 5 』 7 労 r1 6 1 1 5 1 8 4 2 2 8 Ma xd 《 < 9 : 3 : 3 : 1 1 2 8 . 2 5 4 2 . 7 5 4 2 . 7 5 3 1 4 . 2 5 2 2 8 . 0 0 6 8 . 0 7 8 <. 0 1 l ! : e1 6 . 5 7 % 1 5 3 .6 5 1 7 .3 5 1 7 .3 5 3 9 .6 5 2 2 8 .0 0 1 . 4 9 9 . 7 .5 B t r dt e s t e rl L i n k a ~....・・・.・・・・・・...........・・・・・ー・・・・・.............・・・・・・・・ 4・ ................u..........・・・・~...........・・ a ・・・・・・ a ・・・a ・・・・・・・・・・・・・・・・ー・・-・・・・・・・・・・・・・ 4 ・・・・・........-_._-.--_...... W e i g h ta v e r a g ev a l u eo fr e c o m b i n a t i o n( % ) :1 7 . 0 1土1.6 1 1 1 d e n m e l o na r emucht h esame a st h o s eo b t a i n e di nt h e Kagoshima Go ,n a t u r a l l y .t h er e c e s e x p e r i m e n tundero u t d o o rc o n d i t i o n so ff a l l s o w i n g s i v ee a r 1 ygene r e c o g n i z e d under s h o r t d a yi sno o t h e rt h a nt h eg e n e eιAst oMari ,i ti sn o td i f f i c u 1 tt odrawt h esamec o n c l u s i o n .thougk ag e n e t i ca n a 1 y s i s has n o t been made undert h eo u t d o o rc o n d i t i o n s . ,noo n er e c o m b i n a n th a v i n gd o u b l er e c e s s i v egen 回 f o re a r l y Furthermore h e a d i n gandt h i r do u t e rglumec h a r a c t e r sh a sbeenfoundi ne i t h e rt h e 田 o rt h eMarix F2 so ft h e Kagoshima Go1denme1onx B t r dt e s t e rc r o 出 t e rc r o s s ,suggestingthattherei sac l 伺 el i n k a g ebetweene a B t r dt k andt r df o rt h et h i r do u t e rg l u m e . ( 3 )Y e l l o w i n gR e s t o n s et ot h eS t e c i a l合 o ωt hC o n d i t i o n Yasudae ta l .( 1 9 6 5 ) found t h a t when t h es e e d l i n g so f8 0凶 r l e y 白 t oa c o n d i t i o no f8t o1 2hour c u l t i v a r s were s u b j e c t e ds e v e r a 1t i m L i n k a g ea n dP l e i o t r o p i cE f f e c t so ft h eB a r l e yE a r l i n e s sG e n eeak 21 p h o t o p e r i o da t lQoC orlowerduringl i g h t開 r i o danda t200C orh i g h e r duringdark p e r i o d,on1yseven v a r i e t i e s soon yellowedc o n s p i c u o u s 1 y , whereasa l lo t h e rv a r i e t i e st e s t e dd i d notshowsucha r e s p o n s e . However ,i twas confirmed by f u r t h e rs t u d i e st h a ttwoo f seven v a r i e t i e s ( K i n a i5andKagoshimaGo1denme1on)a1wayse a s i l yturnedye l I owunder t h es p e c i a lgrowth c o n d i t i o n and showeda h i g h e rdegreeo f yellowing a scomparedwitht h eotherf i v ev a r i e t i e s . Thec h 1 o r o p h y l lc o n t e n t so f t h e s etwov a r i e t i e sweres i g n i f i c a n t l y10werthant h eo t h e r swhengrown undert h es p e c i a lc o n d i t i o n,butt h ec h 1 o r o p h y l li n c r e a s e dr a p i d 1 y when t h etwov a r i e t i e swerereturnedt ot h eo t h e rgrowthc o n d i t i o n s . Tab1e5 shows t h ec h l o r o p h y l lc o n t e n t so fthet h r e eb a r l e yc u l t i v a r s( K i n a i5 . Kagoshima Go1denmelon and Iwate Mensury C with 1 a t e maturity) grownunderf i v ed i 町e r e n tc o n d i t i o n s . Asseeni nTable5 ,whengrown TABLE5 C h 1 o r o p h y l lc o n t e n t( μ g / l gf r e s h1 ,回f )andt h er a t i oo fc h 1 o r o p h y l la t obo ft h r e ev a r i e t i e sgrownu n d e rd i f f e r e n tf i v ec o n d i t i o n s V a r i e t y C h l o r o p h y l l L l :Dh 2bd a y 1 L h :D l L h :Dh 2 4 hd a y Lh Openf i e l d * 0 . 8 5 7 8 0 . 4 1 0 3 0 . 9 4 2 0 1 .4 1 2 7 1 .0 0 3 7 a 0 . 2 5 9 4 0 . 0 9 1 5 0 . 2 8 5 2 b 0 . 4 0 1 4 0 . 3 1 1 9 1 .2 2 7 2 1 .1 1 7 2 0 . 5 0 1 8 1 .3 1 5 6 T o t a l 1 . 8 1 4 1 3 . 5 2 3 . 3 1 4 . 4 8 3 . 2 2 3 . 3 0 a / b K a g o s h i m a a 0 . 4 2 2 6 1 .1 3 6 7 0 . 8 7 3 8 0 . 8 1 2 1 0 . 8 2 8 1 お4 0 . 0 9 4 8 0 . 3 0 . 2 G o l d e n m e l o n b 河 5 7 2 0 . 2 4 0 4 0 . 2 3 7 1 0 . 5 1 7 4 1 .4 6 0 1 1 . 1 4 1 0 1 .0 5 2 5 1 . 0 6 5 2 T o t a l 4 . 4 6 3 . 5 1 3 . 4 9 3 . 2 7 3 . 3 8 a / b 1 . 0 4 2 2 a 1 . 0 3 0 9 1 .6 2 1 3 1 . 3 8 5 5 1 .0 8 1 9 I w a t e 0 . 3 2 0 1 M e n s u r yC b 0 . 4 9 8 7 0 . 4 7 9 6 0 . 3 6 2 7 0 . 3 0 4 6 1 .4 4 4 6 1 .3 4 6 8 1 . 3 5 1 0 T o t a l ∞ 1.8651 2 . 1 2 3 . 2 2 3 . 2 5 2 . 8 9 2 . 9 8 3 . 4 2 a / b ,D:d a r kp e r i o d ,1 :l o wt e m p e r a t u r e .h :h i g ht e m p e r a t u r e L:l i g h tp e r i o d N a t u r a ld a y l e n g t ha n dt e m p e r a t u r e K i n a i5 本 undert h es p e c i a 1c o n d i t i o n ,both Kinai5and Kagoshima Go1denme1on showeda t o t a lchlorophy l Icontentsmuchl e s sthanIwateMensuryCand h e r e werel i t t l eor weremuchh i g h e ri nc h l o r o p h y l la/br a t i o,whereast nod i 百 ' e r e n c e sbetween t h et h r e ev a r i e t i e s under theoiherf o u rgrowth c o n d i t i o n s . P l a t e1showst h emarkedchangei np l a n tc o l o ro fK i n a i5 . Moreover ,froma 1 a t e rstudyMari,aSwedishe a r l ytwo-rowedc u l t i v a r , andKindoku ,another very e a r l y two-rowed c u l t i v a ri n t r o d u c e dfrom l Iowundersuch Germanya l o n gtimea g o . werea 1 s odemonstratedt oye t h es p e c i a 1 growthc o n d i t i o n . 2 2 s .Yasuda P l a t e1 .P l a n tc o l o ro fthe s e e d l i n g so fKinai5grownundertwo d i f f e r e n tc o n d i t i o n s . i g h tp e r i o dand Right: 1 2 h o u rp h o t o p e r i o da t1 00C during l 0 e r i o d a t2 0C duringdarkp Le f t : n a t u r a lc o n d i t i o n so fMarch G e n e t i ca n a l y s e swerer n a d eo ft h ey e I I o w i n gr e s p o n s et ot h es p e c i a l ,K a g o c o n d i t i o nu s i n gs e v e r a lc r o s s e so fy e l l o w i n gv a r i e t i e so fK i n a i5 s h i r n aG o l d e n r n e l o nand Mariw i t hnon-yeI 1 owingv a r i e t i e sh a v i n gs o r n e r n a j o rg e n e sa l l e l i ct ot h eg e n e so ft h e s ev a r i e t i e s . Ast ot h ec r o s s e s ,t h eh y b r i ds e e d swere s u b j e c t e dt ol o w t e m p e r a w i t hw i n t e rv a r i e t i e s t u r e( 50C o rl o w e r )f o r4 0d a y sp r i o rt op l a n t i n g .A f t e ri n v e s t i g a t i o n f o ry e I l owingundert h es p e c i a lc o n d i t i o n ,t h ep l a n t swerer n o v e dt ot h e l o n g d a yc o n d i t i o ni nw h i c ht h e y were r e a r e dt om a t u r i t y . P l a t eI I P l a t eI I . Se g r e g a t i o nofy e l l o w i n g andnon-yellowingtypep l a n t si n Fao fac r o s so fKinai5withB-trdt e s t e r( n o n y e l l o w i n g ) . TheF a p l a n t sweregrownunder1 2hourp h o t o p e r i o da t1 00Cduringl i g h t e r i o d . p e r i o danda t2 00C duringdarkp L i n k a g ea n dP l e i o t r o p i cE f f e c t so ft h eB a r 1 e yE a r l l n e s sG e n eea~ 23 showst h es e g r e g a t i o no fy e l l o w i n gandn o n y e l l o w i n gt y p ep l a n t si nF2 o fac r o s so fK i n a i5 w i t ha n o n y e l l o w i n gv a r i e t y . With r e g a r dt o c h J o r o p h y l 1c o n t e n t ,t h o s eo fs e g r e g a n t si n F2 o fac r o 話 加t w e e nK i n a i 5andMuyδjiw i t hn o n y e l l o w i n gweremeasuredbyac h l o r o p h y l 1m e t e r d e v e l o p e dbyI n a d a( 1 9 6 4 ) . Themeanc h l o r o p h y l lc o n t e n t so fye l 1 0wing 2 andn o n y e l l o w i n gp l a n t swere0 . 7 7土0 . 0 3 2a nd3 . 2 3土0 . 0 6 6( μgjcm e )r s p e c t i v e l y( F i g .3 ) . Y e l l o w i n gp l a n t si n d i c a t e dac h l o r o p h y l 1c o n t e n to f o n ef o u r t ho rl o w e rt h a nn o n y e l l o w i n gp l a n t s . 骨 2 0 ω g 伺 " 。10 嗣 h ω 』 E Z 。 5 1 3 1 7 2 1 25 2 9 3 3 C b l o r o p b y l lc o n t e n t s 3 7 4 1 45 49(x1 1 1 : 叫 10m ワ F i g .3 .F r e ql1e n c yd i s t r i bl1t i o ns h o w i n gt h ec h l o r o p h y l lc o n t e n to ft h e F y d r i dp l a n t sa n dt h e i rp a r e n t si nac r o s sb e t w l 僧 nK i n a i5 sh ( y e l l o w i n g )a n dM u y δ j i( n o n y e l l o 柄n g ) .g r o w nu n d e rt h es 戸c i a l c o n d i t i o n . Thei n t e r r e l a t i o n sbetweent h en o r m a lo rn o n y e l l o w i n gv e r s u sy e l 田s l o w i n gp l a n t sandt e nv i s i b l ec h a r a c t e r si n v o l v e di nt h e s ee i g h tc r o s weret h e ni n v e s t i g a t e d . Ther e s u l t sa r eshowni nT a b l e6 . Asa p p a r e n t fromt h i st a b l e ,t h eg e n ef o ry e l 1 0wingwasi n h e r i t e di n d e p e n d e n t l yo f nf o rnakedk e r n e l(chromosome1 ),Vf o rtwo-rowedandl if o rl i g u l e ,u zf o rs e m i b r a c h y t i c and a lf o ra l b i n o lemma l e s s(chromosome2 ) ( c h r o m o s o m e3 ) ,K f o rh o o d e da p p e n d a g e and g l 3f o rg l o 田 ys e e d l i n g ,0 f o ro r a n g elemma (chromosome6 ),sf o rs h o r t h a i r d (chromosome4 ) r a c h i 1 l a and rf o r smooth awn (chromosome7 ) . Table7 shows t h e , KagoshimaGoldenmelonand r e s u l t so b t a i n e di nt h r e ec r o s s e so fK i n a i5 Mariw i t hamarkers t o c k ,B l a c k b r a c t e a t e( B t r d ) . I ti sa p p a r e n tfrom t h i st a b l et h a tt h eg e n e sf o rn o r m a l andy e l l o w i n ga r el i n k e dw i t h Bb f o rb l a c kandw h i t elemmaandw i t hTrdtrdf o rnormalandt h i r do u t e r glumeonchromosome5,and t h a tr e c o m b i n a t i o nv a l u e st h u so b t a i n e d a r emucht h esameamongt h 白 ec r o s s 回. W eighteda v e r a g ev a l u e so fP o b t a i n e dbyt h et h r e ep o i n tt 邸 t were1 1 . 2 1土3 . 2 6 5% f o ry e l l o w i n ggene ,21 .37土1.557必 f o rye l 1 0winggene and B,and1 1 . 5 6土3 . 2 4 2% andtrd f o rtrdandB . A lthough t h er e c o m b i n a t i o nv a l u ef o ry e l l o w i n ggene 24 S .Y a s u d a TABLE 6 S e g r e g a t i o nf o rn o r r n a l( X )v e 四u sy e l l o w i n g( x )andt e no t h e rc h a r a c t e rp a i r s i nF2S o ft h ec r o s 記 sw i t hK i n a i5andK a g o s h i r n aG o l d e n r n e l o n Cr ,国民dw i t h S x y m b o l C h r o m o - x K i n a i50r o t a l o m e Y y Y y T K a g o s h i m aG . Y y s R u s s i a n8 2 V U l AJ a 。。 u r a n g elemma V S U s 2 3 2 6 7 2 M u y o j i j Li J G ω e s h i k o k u S a i1 G l . g l . 3 4 R r 7 N S n s N n 1 7 1 7 V U M i n n .9 0 5 N i g r i n u d u m N i g r i n u d u m * Co l 鈴 s s1 ' " Uz uz s s h s s K 事 2 4 7 2 12 1 6 3 6 49 8 1 0 4 3 6 91 1 0 1 1 13 9 6 2 9 3 4 3 99 1 2 23 1 5 9 5 3 3 01 1 8 1 0 84 5 6 0 1 3 1 61 1 4 1 1 53 4 5 7 9 1 7 06 5 5 22 3 3 1 0 1 1 84 3 4 7 8 2 1 6 1 2 04 1 4 11 4 2 1 6 9 4 1 1 2 3 97 0 回 1 1 0 43 7 4 61 2 1 9 9 1 5 94 3 5 61 4 2 7 2 3 1 6 22 9 3 1 9 1 81 3 1 3 3 6 72 5 2 χi P χ2f o rYy ( 3 :1 ) 1 . 9 9 0. 2 . 1 0 . 7 1 5 ・. 3 0 . 4 0 0 0 . 5 7 9. 5 0 . 3 1 4. 7 . 5 0 . 1 6 2 0 . 6 0 1. 5 . 3 0 . 0 6 7 0 . 8 1 1. 5 . 3 0 . 1 6 6 0 . 2 4 2. 7 . 5 0 . 1 0 8 3 .2 9 2. 1 .0 5 0 . 0 2 5 ) . 9 0 . 0 2 5 。 0 . 6 4 9 .5-. 3 0 . 0 0 7 0 . 7 6 4. 5 . 3 1 .回 4 0 . 0 5 9 .9-. 8 0 . 0 7 8 1 . 1 6 1 .3-. 2 2 . 1 4 0 0 . 5 2 2. 5 . 3 2 . 4 0 9 . 4 3 2. 0 2 . 0 12 . 2 2 9 5 81 7 2 01 4 1 0 9 5 日 2 2 27 7 1 0 9 0 . 9 8 0. 5 . 3 2 . 2 2 9 7 . 5 2 . 2 2 9 .羽5 . 5 61 9 2 41 0 1 0 9 O C r o s s e dw i t hKag o s h i m aG o l d e n m e l o n l i g h t l yl a r g e rthant h evalueso b t a i n e di nexperimentsunder andB wass ,theresultsobtainedheremaysuggest outdoor-andshort-dayc o n d i t i o n s ι. t h a tt h egenef o ry e l l o w i n gi st h esamease o p u l a t i o n s Furthermore.progenyt e s t so fp l a n t sd e r i v e dfromt h eF2 p grownunderoutdoorc o n d i t i o nwerec a r r i e doutundert h es p e c i a lgrowth 1 a r i f yt h er e l a t i o n s h i pbetweenthey e l l o w i n g c o n d i t i o nf o ry e l l o w i n gt oc a " . The results indicated that a totalof99 ear 1 yp l a n t s geneande o p u l a t i o n so ftwoc r o 田e so fK i n a i5x randomly taken from the F2 p 0 5andKagoshimaGoldenmelonx Minn.9 0 5werea l lhomozyMinn.9 l 1 0wingg e n e . T h i r t e e nl a t etypep l a n t sfromF2,ont h e gousf o rt h eye e t e r o z y g o u sandjorhomozygousf o rt h enon-yellowing o t h e rhand,wereh 偲 ef a c t s .t h ey e l l o w i n gr 田 p onse o f bothK i n a i5and g e n e . Fromth Ka goshimaGoldenmelont ot h es p e c i a lgrowthc o n d i t i o ncanb ec o n s i d e r e d 百e c to fe ιon t h ec h a r a c t e r . Therefore ,a p白 duet ot h ep l e i o t r o p i ce s i b l econ c 1 usioni st h a tt h eo r d e ro f arrangemento fy e l l o w i n ggenee a " ando t h e rgenest e s t e d on chromosome5i se a ,,t r d B . Thus,e a " may be l o c a t e da tt h ed i s t a l end o ft h el o n g armo f chromosome5 . A chrom 叩 o memapi sgiveni nF i g .4 . 25 L i nkageandP l e i o t r o p i cE f f e c t so ft h eBarleyEa rUn 鎚 sG e neeak TABLE7 Linkagesbetweenthreegen 田 o fthegenef o ryellowingr 回 c t i o n .Bf o rblack lemmaandt r dforthirdouterglumeinthr田 cro回 目 ofKinai5( 1 ) . Kag 四 h imaGo ldenmelon( 2 )andMari( 3 )witha B-trdt 国 t e r 冨 C r o s s C h a r a c t e r 閏 t e d t x I t em 玄 y Y y Y T o t a l χ2 P 2 4 4 6 1 Obsvd.number 2 ' l : l 0 9 Linkage18.94% 2 0 3 .' l : l 2 6 . 2 3 2 6 . 2 35 O . ' l : l 3 0 6 9 . 8 3 関. 0 00 . 7 3 6 . 4 1 3 6 回 a n a 1 d 1 提 w 0 唱 m b 団 h W a h i V 目 z 《 也 & Y k 2 6 y t M 1 I 1 2 。』v ι number 2 0 2 2 6 G R a v 1 Linkage23.44% 1 9 7 .1 93 1 . 5 6 3 1 .5 64 4 . 6 9 3 0 5 7 . 5 3 0 5 . 0 02 . 0 2 6 . 3 0出 v d .number 2 0 1 3 0 3 2 4 8 Linkage2 2 .' l : l % 2 0 2 .臼 3 0 . 7 1 3 0 . 7 14 6 . 9 8 一 3 1 1 3 1 1 . 0 0O .1 0 4 ).95 WeightedP (船 :2 1 . 3 7 土1 . 5 5 7 1 Obsvd.number 1 5 4 8 2 。 69 7 2 .7 8 0 . 9 7 nv s . 1 G m~ 2i 76 ~~~o~i!~l ?~vd.n~~~~, ~~~ __ ~~ 5 . 3 8 0 . 9 7 ; ; ; : b;ã~t:-- I L inkage1 1 .28% 1 5 3 .4 77 5 . 2 8 7 a t e( Y y ) 1 3O b s v d .number 1 5 1 8 0 1 7 9 Linkage10.82% 1 5 6 . 4 17 6 .8 4 7 6 . 8 4 0 . 9 1 2 9 5 2 9 5 . ∞ 1.部 7 3 0 5 3 0 5 . ∞ 0.028 . 7 . 5 ). 9 5 3 1 1 3 11 .0 00 . 3 8 7. 9 5 -. 9 WeightedP (目的:1 1 . 2 1土3 . 2 6 5 1 Blackv8. w h i t e lemma(Xx) 2 andnormal r a c t . v s .b 国 t e( Y y ) 3 5 1 8 1 7 2 1 Obsvd.number 1 . 9 6 3 .4 67 5 .2 9 7 5 .2 9 0 Linkage11.20% 1 5 3 0 5 3 0 5 . 0凶 v d .number 1 6 2 6 3 1 7 8 Linkage1 2 .田 % 1 日. 2 07 4 . 8 0 7 4 . 8 0 1 . 2 0 3 0 4 3 0 4 . 0 02 .日7 . 5 . 3 5 2 7 8 0国 v d .number 1 8 0 1 Linkage10.91% 1 5 6 . 4 37 6 . 8 2 7 6 . 8 2 0 . 9 3 3 1 1 3 1 1 . 0 00 . 2 8 1 ∞0.616 . 9 . 8 ). 9 5 WeightedP(%):1 1 . 5 6土3 . 2 4 2 f n l e?k B 1 1 .5 6土 3 . 2 4 2% 1 1 . 2 1土 3 . 2 6 5% 2 1. 3 7土 1 . 5 5 7% F i g .4 . A mapshowingarrangemento ft h et h r e egenes onchromosome5andt h ed i s t a n c ebetweent h e m . 26 S .Yasuda I np a r a l l e lt ot h e s ea n a l y s e s ,a l l e l i s mt e s t sf o rt h ey e l l o w i n gg e n e l a n t sfrom weremadeu s i n g1 0o rmoreF lp l a n t sand3 0 0o rmoreF2 p o l d e n m e l o n e a c ho ft h r e ep o s s i b l ec r o s s e s among K i n a i5,Kagoshima G andMar i . N o n y e l l o w i n gp l a n t swere n o tfoundamong t h e s eh y b r i d s whengrownundert h es p e c i a lc o n d i t i o n . Ast oa n o t h e rv e r y ea r 1 yc u l t i v a r s ,Kindoku,i twas c o n f i r m e dt o havet h eg e n ee a i n c ea l l1 0p l a n t so fF land3 0 0p l a n t so fF2 froma h s crωsb e t w e e nKindoku and K i n a i5t u r n e dy e l l o wwhen grown u n d e r t h es p e c i a l∞n d i t i o n . DISCUSSION T a k a h a s h i and Yasuda( 1 9 6 0 ) showed t h a tt h ev e r yea r 1 y ba r 1 ey v a r i e t yK i n a i 5 was d a y n e u t r a lo ri n s e n s i t i v et os h o r t p h o t o p e r i o d, a l t h o u g hEnomoto( 1 9 2 9 )p o i n t e do u tt h a tt h i sv a r i e t ywass e n s i t i v et o t e m p e r a t u r e . A c c o r d i n gt oT a k a h a s h i andYasuda,K i n a i5,a sw e l la s t h eo t h e rv a r i e t i 回 s howsa c c e l e r a t e dh e a d i n gt i m ew i t har i 記 i nt e m p e r a t u r ew i t h i nac e r t a i nt e m p e r a t u r er a n g e(8-230C ),s u g g e s t i n gt h a t 1 ey t h e r ea r eno d i f f e r e n c e si nt e m p e r a t u r es e n s i t i v i t y among t h e bar 関, i n c 1u d i n gK i n a i5undero r d i n a r ygrowthc o n d i t i o n . F u r t h e r v a r i e t i more ,i twasc o n f i r m e dt h a tt h es e n s i t i v i t yt ot h es h o r t p h o t o p e r i o dwas 1 1 i ng t h eh e a d i n gt i m eo ff a l l s o w n ba r 1 e yi n t h ec h i e fa g e n tc o n t r o c e n t r a land s o u t h w e s t e r nJ a p a n( T a k a h a s h iandY a s t i d a ,1 9 5 8 ) . From t h 田 ef i n d i n g s ,i tmayn a t u r a l l yb ee x p e c t e dt h a tt h eg e n ee 仇 f o u n di n K i n a i5f o rv e r ye a r 1 yh e a d i n gu n d e rt h ef a l l s o w nc o n d i t i o ni sr e s p o n 回 f o rt h en o n s i b l ef o rt h ei n s e n s i t i v i t yt os h o r t p h o t o p e r i o d and a l S戸 c i f i cr e s p o n s et ot e m p e r a t u r e . I tmaybeo fi n t e陀 5 tt on o t et h a tt h eg e n eea sr e s p o n s i b l ef o rt h e ki 戸c i a ls h o r tp h o t o p e r i o d i cc o n d i t i o ni nwhich y e l l o w i n gr e a c t i o nt ot h es i o dandh i g hd u r i n gt h e t e m p e r a t u r ewask e p tlowd u r i n gt h el i g h t戸 r d a r kp e r i o d . R o b e r t s( 1 9 4 8 )f o u n dt h a tsuchas p e c i a lgrowthc o n d i t i o n moreo rl e s sd i s t u r b e dt h ep r o d u c t i o na n d / o ra c c u m u l a t i o no fc h l o r o p h y l l i np l a n t s . I tmaybe c o n s i d e r e d ,t h e r e f o r e ,t h a tt h egenee 仇 , i smore s e n s i t i v et ot h es 開c i a lgrowth c o n d i t i o n comparedw i t ht h eo t h e re a r l i n e sgen 田. Furthermore ,ap o i n tworthyo fm e n t i o n e dh e r ei st h a tt h eS w e d i s h ba r 1 e yMari ,av e r ye a r l ymutant fromBonus( G u s t a f s s o ne ta l .1 9 6 0 ), r 回 p o n d e dt ot h es 戸c i a lgrowthc o n d i t i o ni nt h esamemannera sK i n a i 5,a n dt h a tt h eg e n ee a a 'i nMarimayb ei d e n t i c a lw i t he ιinK i n a i5 . Namely ,F a v r e tandF r e c h a( 1 9 6 7 )c a r r i e do u ta l l e l i s mt 回 tf o rr e c 回s i v e e a r l i n e s sgen 田 u s i n gF lp l a n t so fd i a l l e l i cc r o 路 e samongK i n a i5 ,Mari , A t s e landM.C .20SP( i nducedfromM.H e d a ) . TheF lp l a n t so fK i n a i 5xMaric r o 器 b e h a v e da sane a r l yh e a d i n gt y p es i m i l a rt ot h e i rp a r e n t s , 四 L i nkageandP l e i o t r o p l cE f f e c t so ft h eB a r l e yEar U n e s sGenee a / c 27 i n d i c a t i n gt h a tt h er e c e s s i v ee a r l i n 缶 sg e n ei n v o l v e di nK i n a i5wast h e mea st h a ti nM a r i . M o r e o v e r ,t h ee a r l i n e sg e n e si nt h eo t h e rtwo m l i n ge ta l .( 19 6 6 ) v a r i e t i e sweren o tt h esamea st h a to fK i n a i5 . Dor c o n d u c t e de x p e r i m e n t st of i n dd i 百 ' e r e n c e si ngrowthande a rf o r m a t i o n nusi nv a r i o u sc o m b i n a t i o n so f b e t w e e nMariandi t so r i g i n a lv a r i e t yBo d a y l e n g t hand t e m p e r a t u r e . Bonusr e m a i n e dp u r e l yv e g e t a t i v e when -d ay ( 8 h o u rd a y )c o n d i t i o n ,w h i l e Mari a l w a y s grown underas h o r t -d ayands h o r t d a yc o n d i t i o n s .I no t h e r h e a d e dn o r m a l l yunderb o t hl o n g ,Bonusr e a c t e da sap r o n o u n c e dl o n g d a yp l a n t ,w h i l s tMariwas words i n s e n s i t i v et op h o t o -andt h e r m o p e r i o d s . Ast ot h ea n o t h e rtwo c u l t i v a r s . Kagoshima G o l d e n m e l o no fJ a p a n r m a n y .t h er 回 s o n sf o rb o t hh a v i n gt h e回 mee a / & andKindokufromGe g e n ea st h a to fK i n a i5a r es t i l 1o b s c u r e . 田 i sm a r k e d l ym a n i f e s t e da tt h e S i n c et h es p e c i a ly e l l o w i n gr e s p o n S問 d 1 i ngs t a g e ,t h i sr 関 p on 田 i sc o s i d e 陀 dt ob el1s e f u lf o ri d e n t i f y i n gand 鵠 l e c t i n ge a r l yp l a n t sw i t he ιatt h es e e d l i n gs t a g ei nbar 1 eyb r e e d i n g 白. P l e i o t r o p i ce f f e c t so fe ιon t h ey i e l dandy i e l dc o m p o n e n t s p r a c t i r e m a i nt ob es t u d i e si nt h ef u t u r e . 回 SUMMARY Ther e c e s s i v ee a r 1 i ne 弱 g e n ee ιinvolv 吋 i ntwo-rowedba r 1 eyc u l t i v a rK i n a i5p r e v i o u s l y found by Yasudaand Shimoyama ( 1 9 6 4 )was r e s p o n s i b l ef o rt h ev e r ye a r l yh e a d i n gn o to n l y undert h ef a l l s o w i n g c o n d i t i o ni nt h eo p e nf i e l db u ta l s oundert h es h o r t p h o t o p e r i o d i c∞n ,e a / &i sr e g a r d e dr a t h e ra sag e n e d i t i o na th i g ht e m p e r a t u r e .T h e r e f o r e ,K i n a i 5∞n i n s e n s i t i v eo rd a y n e u t r a lt od a y l e n g t h . F u r t h e r m o r e 鴫 nw hengrownundert h e∞n d i t i o no f8 -t o s p i c u o u s l yt u r n e dy e l l o w g r )d u r i n gt h el i g h t 1 2 h o u rp h o t o p e r i o da tlow t e m p e r a t u r e( b e l o w1 00C 0 rh i g h e r )d u r i n gt h edarkp e r i o d . p e r i o dand a th i g ht e m p e r a t u r e( 2 0C o Thei n d u c e dy e l l o w g r e e ns e e d l i n g ss o o nr e c o v e rt og r e e nwhenmoved twasc o n f i r m e dfroma n a l y s e so fF za n d / o r t oo t h e rg r o w t hc o n d i t i o n s . I 話e so fK i n a i5w i t hn o n y e l l o w i n gc u l t i v a r st h a t F 3g e n e r a t i o n so ft h ec r o 回 p on 田 w as a t t r i b u t e dt ot h ep l e i o t r o p yo fe a / & ・ T he t h i sp a r t i c u l a rr r 1 yc u l t i v a r s . Kagoshima G o l d e n m e l o n from o t h e r two-rowed v e r ye a ,Mari fromSwedenandKindokufromGe rmany Kagoshima p r e f e c t u r e 1 i sm a l lt u r n e dy e l l o w g r e e nundert h es p e c i a lgrowthc o n d i t i o n . Thea l l e 田t si d i c a t e dt h a tt h er e 句 s s i v ee a r 1 i n e s sg e n ei n v o l v e di nt h e 田 t h r e e t l 1i n d e n t i c a lw i t he a / & . U s i n gt h ec r o 田e so fK i n a i5 , v a r i e t i e s were a KagoshimaG o l d e n m e l o nanda l s oM a r iw i t hsomel a t ematuring1 i nkage h e1 i nkageo fe a / &was s t u d i e di nt h ef a l l s o w i n gc o n d i t i o ni n t e s t e四, t i e l d .t h es h o r t p h o t o p e r i o d i cc o n d i t i o ni nag r e e n h o u s eandi n an0伊 n f 配i a lg r o w t hc o n d i t i o nf o ry e l l o w i n g . Ther e s u l t si n d i c a t e dt h a t t h es p 28 S .Yasuda g e n ee a "wasonchromosome5,t h er e c o m b i n a t i o nv a l u e sf o re a , ,-Bb e i n g 1 6 . 7 8 21 . 3 7p e rc e n tand. t h a tf o re a , t r d1 1 . 2 1p e r偲 nt . Theo r d e ro f 関 eg e n e sonchromosome5wase a ,,t r d B . a r r a n g e m e n to ft h 注目 ACKNOWLEDGEMENTS Th ea u t h o rwish 回 t oexpr 凶 s h l ss i n c e r ea p p r l c i at I ont oD r .R .T a k a h a s h l .Emerltus P r o f e : 掲o r ,Okayama Unlversity,for c r l t i c a 1r e a d l n go ft h e manuscript andmaking 回 i n d e b t e dぬ D r .T .K o n i s h lo ft h eI n s t i t u t ef o r v a 1 u a b 1 es u g g e s t i o n s . Thea u t h o rI sa 1 A g r l α l l t u r a landB i o 1 0 g l c a 1S c i e n c e sandMr.Y .Oklnagao ft h eU n l v e r s i t yH o s p i t a 1f o r t h e i ra s s l s t a n c e sdurlngt h ec o u r s eo ft h i ss t u d y . TURECITED L I τ'ERA Do rm 1 i n g .1 . .G u s t a f s s o n .A . .J u n g .H.R .andW e t t s t e i n .D .v o n .1 9 6 6 .P h y t o t r o nc u l t i v a t i o no fS v a 1 o f ' sBonusb a r 1 e yandi t smutantS v a 1 ぽ sMar i .H e r e d l t a s .5 6 :2 2 1 2 3 7 . Enomoto.N .1 9 2 9 . On t h ep h y s i o 1 0 g i c a ld i f f e r e n c e between t h es p r l n g andw i n t e r r .Exp.S t a . .Tokyo.1 :1 σ 7 1 3 8( i nJ a p a n e s e t y p e si nwheatandb a r l e y .J .Imp.Ag wlthE n g l l s hsummary). F a v r e t .E .A.andF r e n c h a .J .H .1 9 6 7 . A l l e 1 i sm t e s to fg e n e sf o re a r 1 i n e s s . B a r 1 ey N e w s 1 e t t e r .1 0 :1 2 1 . Gustaf 鎚 ο n .A . .Hagberg.A.andL u n d q v l s t .V. 1 9 6 0 . Thel n d u c t i o no fe a r l ymutants i nBonusb a r 1 e y .H e r e d l t a s .4 6 :6 7 5 6 9 9 . I n a d a .K. 1 鈴4 .S t u d i e sonamethodf o rd e t e r m l n i n gt h ed e e p n e s so fgreenc o 1 0 rand c h 1 0 r o p h y l lc o n t e n to fi n t a c tc r o p1 e 町田a ndi t sp r a c t i c a 1app1 i c a t i o n s .2 .P h o t o e 1 回 t r i cc h a r a c t e r so fc h 1 0 r o p h y l l o m e t e randc o r r e 1 a t i o nbetweent h er e a d i n gand 朗 v e s . Pr ∞ .Crop.Scl .S凹 .J a p a n .3 3 :ぬ 1 3 偲.( i nJapan 艇;e c h l o r o p h yl 1c o n t e n tI n1 w i t hEng 1 i shsummary). 印 C h 1 0 r o p h y l lf o r m a t i o nI nseedUngso fZoamaysL .A r c h .BI ∞hem. K o s k i .V.M. 1 9 2 9 :3 3 9 3 4 3 . ∞mingandpigmentcontent. PlantPhysio1..23:379-お7 . R o b e r t s .R .H. 1 9 4 8 .B 1 T a k a h a s h i .R .andYasuda.S .1 9 日. Ge n e t i cs t u d i e sonheadingd a t ei nb a r 1 e y .“ S t u d i e s ont b eb u 1 kmetb 吋 o fp 1 a n tbreeding"Edi t e dbySa k a i .TakabashiandAkemine. 4 4 6 4 .Yokendo.Tokyo.( i nJ a p a n e s e ) . T a k a b a s h i .R .andY a s u d a .S .1 9 6 0 .V a r i e t a 1d i f f e r e n c e si n responωs t op b o t o p e r i o d and t e m p e r a t u r ei nb a r 1 e y . Ber .OharaI n s t .1 a n d w .B i o l . .OkayamaU n i v e r s i t y . 1 1 :おら3 8 4 . Y a s u d a .S .andShimoyama.H. 1 鈍4 . P b y s i o 1 0 g y and g e n e t i c so fe a r emergence i n b a r 1 e yandw h e a t .V 1 .A n a 1 y t i c a 1s t u d i e so ft h eg e n e sf o rheading t i m eo ff a l l sownb a r l e y s . NogakuKenkyu.5 0 :1 6 7 1 邸 ' .( i nJapan e : 抱 : ) . Y a s u d a .S . .K o n i s h i .T .andShimoyama.H. 1 9 6 5 .V a r i e t a ld i f f e r e n c e si ny e l l o w i n go f bar 1 e y sundeac e r t a i nc o n t r o l I edc o n d i t i o no ft e m p e r a t u r eaudp h o t o p e r i o d .and i t smodeo fi n h e r i t a n c e . NogakuKenkyu.5 1 :5 3 6 5 .( i nJapane 記 : ) .
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