佐 賀 大 学 農 学 実 報 第2 3号 1∼6 OnanewStubbyRootNematode( T r i c h o d o r u s k u r u m e e n s i sn .s p . )fromKyushu,Japan By TamioYoKoo Laboratoryo fP l a n tP r o t e c t i o n F a c u l yo fA g r i c u l t u r e ,SagaU n i v e r s i t y Nematodesb e l o n g i n gt ot h e genus T r i c h o d o r u swhichf o u n dfromKyushu,Japan amountedt ot h ef o l l o w i n g3s p e c i e sh i t h e r t o . ( 1 ) T r i c h o d o r u sm i r z a iS I D D I Q I ,1960 ( 2 ) T .c e d a r u sYoKoo,1964 ( 3 ) T .l o n g i s かl u sYoKoo,1964 I n1 9 6 5 ,F e b r u a r y ,If o u n danews p e c i e so ft h egenusT r i ・ c h o d o r u sfromt h es o i l s aroundt h er o o t so ft h ePrunusp l a n t e di nt h ePrunus Orcharda tt h eKyushuH o r t i c u l t u r a lE x p e r i m e n t a lS t a t i o n ,FukuokaP r e f e c t u r e ,Kyushu. Thiss p e c i e si sd i s t i n g u i s h e dbyc o m p a r a t i v e l ys m a l ls i z e( 0 . 5 0 . 6 m m ) . Themale h a v i n gt h r e eventromedianc e r v i c a lp a p i l l a ea n t e r i o rt ot h ee x c r e t o r yp o r e ,p o s t e r i o r t ot h eb a s eo fO n c h i o s t y l e ,c o m p a r a t i v e l yp o s t e r i o re x c r e t o r yp o r ea tal e v e lo fa n t e r i o r p a r to ft h eo e s o p h a g e a lp o s t e r i o rs w e l l i n g ;t h r e eventromedians u p p l e m e n t a r yp a p i l l a e , o n eo f which i sw i t h o u tt h es p i c u l a rr e g i o n ;s t r i a t e dc o m p a r a t i v e l ys m a l ls p i c u l a ; gubernaculumw i t has m a l lh o o k l i k ee n l a r g e m e n ta td i s t a le n d ;andf e m a l ehavingt h e p a i r e da s y m m e t r i c a lg o n a d s ;a n t e r i o rgonadi sc o m p a r a t i v e l ys h o r t e r( a b o u t84% o f t h el e n g t ho fp o s t e r i o rg o n a d ) ,p o s t e r i o rgonadl o n g e rw i t hr e f l e x e do v a r y ;ands p i n d l e s h a p e ds p e r m a t h e c a . T r i c h o d o r u sk u r u m e e n s i sYoKoo,1966n .s p . D i m e n s i o n s : Female:( n= 1 0 ) L= 0.50mm(0.35-0.68mm); a=1 4 . 7( 1 1 . 6 1 8 . 6 ) ;b= 3 . 8( 2 . 7 4 . 7 ) ;c= s u b t e r” m i n a l ; e= 5 . 1( 3 . 8 6 . 8 ) ; V= 5 5 . 8% ( 5 5 . 1 6 1 . 3%); G1=18.5% ( 1 5 . 4 2 1 . 0%), G2= 2 2 . 1% ( 1 8 . 1 2 6 . 4%);s t y l e t= 5 . 3 μ( 4 7 . 5 5 2 . 5 μ ) H o l o t y p eFemale;L= 0.52mm;a=1 4 . 9 ;b= 4 . 0 ;c= s u b t e r m i n a l ;e= 6 . 8 ;s t y l e t =5 0 . 5 ;V =52.0%;G1=15.4%;G2= 26.0%. M a l e ;( n= 2 ) L= 0.59mm(0.57-0.61mm);a=1 3 . 4( 1 2 . 6 1 4 . 2 ) ;b= 4 . 4( 4 . 3 4 . 5 ) ;c= s u b t e r m i n a l ;e= 5 . 8( 5 . 5 6 . 0 ) ;T= 52.9%( 5 2 . 7 5 3 . 0 % ) ;s t y l e t=53.4μ ( 5 2 . 5 5 4 . 0 μ ) ; Spicula=46.3μ ( 4 2 . 5 5 0 . 0 μ ) ; gubernaculurn=14.5μ ( 1 3 . 0 1 6 . 0 μ ) ; T=52.9% (52.7-53.0%) AllotypeMale; L=0.57mm; a=12.6;b=4.5;c=subterminal;e=6.0;s t y l e t = 5 4 . 0 μ ;Spicula=42.5μ;gubernaculurn=13.0μ;T=52.7% 2 佐 賀 大 学 農 学 集 報 第2 3号 Female: Bodyc y l i n d r i c a l ,curveds l i g h t l yv e n t r a l l ywhenk i l l e dbyh e a t . T a i lt i pr o u n d . Anus s u b t e r m i n a l . L i pr e g i o nd e s k l i k es h a p e d . F i r s ta n n u l el a r g eandi t so u t s i d es l i g h t l y p r o j e c t . E x c r e t o r yp o r ea tal e v e lo fa n t e r i o rendo fp o s t e r i o ro e s o p h a g e a ls w e l l i n g . Oesophaguscomposedo ftwop a r t s ;a n t e r i o r l yas l e n d e rt u b ewhichf o l d stwot i m e s , expandingt op o s t e r i o re l o n g a t ec o n o i ds w e l l i n g . Vulval o c a t e da ta b o u t56% o ft o t a l bodyl e n g t h ,s h o r tt r a n s v e r s es l i t . C u t i n i z e dp i e c e ss u r o u n dv a l v as m a l l ,c o n s p i c u o u s . Gonadp a i r e d ,o p p o s i t e d ,a s y m m e t r i c a l l y .P o s t r e r i o rgonadl o n g ,4o r5t i m e so fv u l v a l bodyw i d t h ,t e r m i n a lp a r to fo v a r yr e f l e x e d .A n t e r i o rgonads h o r t ,o u t s t r e c h e d ,2o r3 t i m e so fv u l v a lbodyw i d t h . Spermathecas p i n d l e s h a p e d . Vaginaa b o u t2/5-1/2o f v u l v a lbodyw i d t h ,c y l i n d r i c a l . I nt e r m i n a lnarrowp a r to fovaryo o c y t e si no n er o w . I ns u b t e r m i n a lp a r to o c y t e si ntwor o w .Caudalp o r es u b t e r m i n a l . M a l e : Bodyc y l i n d r i c a l ,t a p e r i n gt ot h eb a s eo fo n c h i o s t y l ea si nf e m a l e . T a i lt i pr o u n d . C l o a c as u b t e r m i n a l ,p o s t e r i o rp a r to fbodyhooksv e n t r a l l ywhenk i l l e dbyH e a t .E x c r e t o r yp o r es i t u a t e dn e a rp o s t e r i o rp a r to fo e s o p h a g e a ls w e l l i n g . Threeventromedian c e r v i c a lp a p i l l a el o c a t e da n t e r i o r l l yt oe x c r e t o r yp o r e . F i r s tp a p i l l a es i t u a t e db e h i n d t h eb a s eo fo n c h i o s t y l e . Thirdp a p i l l a eabovee x c r e t o r yp o r e . Secondp a p i l l a el o c a t e d a tal e v e lo fmidwayo ft h e s etwop a p i l l a e . P a i r e ds p i c u l al o n gandv e n t r 叫l yc u r v e d , markedlys t r i a t e dbyf i n et r a n s v e r s es t r i a t i o n so fa n n u l e s . Threel a r g es u p p l e m e n t a l m u s c u l a rp a p i l l a es t a n d i n ga n t e r i o rt oc l o a c a .D i s t a n c ebetweenp a p i l l a el o c a t e dn e a r e s t t oc l o a c aands e c o n dp a p i l l a ei ss l i g h t l ys h o r t e rt h a nt h a tbetweens e c o n dandt h i r d p a p i l l a e . Gubernaculuma b o u t1 / 3l e n g t ho fs p i c u l aw i t hah o o k l i k es m a l le n l a r g e m e n t d i r e c t e ddownwardsn e a rd i s t a lendo fg u b e r n a c u l u m .C a u d a lp o r es u b t e r m i n a l . D i a g n o s i sandR e l a t i o n s h i p : Thiss p e c i e si sd i s t i n g u i s h e dbyt h ec o m p a r a t i v e l ys m a l ls i z e( 0 . 5 0 . 6 m m ) ;t h emale havingt h r e eventromedianc e r v i c a lp a p i l l a e ,a n t e r i o rt ot h ee x c r e t o r yp o r e ,p o s t e r i o r t ot h eb a s eo fO n c h i o s t y l e ;c o m p a r a t i v e l l yp o s t e r i o re x c r e t o r yp o r ea tal e v e lo fan・ t e r i o rp a r to ft h eo e s o p h a g e a lp o s t e r i o rs w e l l i n g ;t h r e ev e n t r o m e d i a ns u p p l e m e n t a r y p a p i l l a e , one o f which l o c a t e dw i t h o u tt h es p i c u l a rr e g i o n ;s t r i a t e dc o m p a r a t i v e l l y s m a l ls p i c u l a ;gubernaculumw i t has m a l lh o o k l i k e de n l a r g e m e n ta td i s t a le n d ; and f e m a l eh a v i n gt h ep a i r e da s y m m e t r i c a lg o n a d s ,a n t e r i o rgonadi sc o m p a r a t i v e l l ys h o r t e r ( a b o u t84~ぢ of t h el e n g t ho fp o s t e r i o rg o n a d ) ,p o s t e r i o rgonadwithr e f l e x e do v a r ya t d i s t a le n d ;s p i n d l e s h a p e dS p e r m a t h e c a . Theknowno t h e rs p e c i e so fgenus T r i c h o d o r u sh a v i n gm a l e switht h r e ev e n t r o m e d i a nc e r v i c a lp a p i l l a eandt h r e evenromedians u p p l e m e n t a r yp a p i l l a e ,andgubernaculum withae n l a r g e m e n ta td i s t a lenda r ef o l l o w i n gs e v e r a ls p e c i e s ,o fwhich T r i c h o d o r u s c e d a r u s YoKoo, 1964 and l o n g i s t y l u s YoKoo, 1964 a r e found from w e s t e r n Japan ( K y u s h u ) . ( 1 ) T r i c h o d o r u sp r i m i t i v u s( n EMAN,1 8 8 0 )MrcoLETZKY,1922 ( 2 ) T .ρ a k i s t a n e n s i sS r n m Q r ,1962 ( 3 ) T .c y l i n d r i c u sHooPER,1962 ( 4 ) T .v i r u l i f e r u sHooPER,1963 ( 5 ) T .c e d a r u sYoKoo,1964 TamioYOKOO: OnanewStubbyRootNematode 3 ( 6 ) T .l o n g i s かl u sYoKoo,1964 Thed i s t r i b u t i o no ft h eventromedianc e r v i c a lp a p i l l a eo ft h i ss p e c i e si sc l o s e l yr e l a t r i c h o d o r u sc e d a r u s ,T .v i r u l i j e r u sand T .l o n g i s t y l u s . Buti nT .c e d a r u s edt ot h a ti nT t h ef i r s ttwop a p i l l a es i t u a t e dw i t h i nt h eo n c h i o s t y l e r e g i o n ,andi nT .v i r u l i f e r u st h e f i r s tonep a p i l l a e ,a n t e r i o rt ot h eb a s eo fo n c h i o s t y l e . Andi nT .l o n g i d t y l u st h ef i r s tp a p i l l a es i t u a t e da ta l e v e lo rb e h i n dt h eb a s eo f o n c h i o s t y l e . Andt h ed i s t r i b u t i o no ft h eventromedians u p p l e m e n t a r yp a p i l l a eo ft h i ss p e c i e si s c l o s e l yr e l a t e dt ot h a to fT .c e d a r u sand T .l o n g i o s かl u s .Butt h ed i s t a n c ebetweent h e .l o n g i s t y l u s ,andt h e l o w e s tp a p i l l a eandmedianp a p i l l a ei sn o ts onarrowa st h a to fT d i s t a n c ebetweent h ef i r s tands e c o n dp a p i l l a ei sc o m p a r a t i v e l l yl o n g e rt h a nt h a tb e tweenmedianandl o w e s tp a p i l l a e . Thep a i r e dgonadso ff e m a l ei sa s y m m e t r i c a li n t h i ss p e c i e sandt h el e n g t ho ft h ea n t e r i o rgonadi aa b o u t84% o ft h a to ft h ep o s t e r i o r g o n a d ,t h es p i n d l e s h a p e ds p e r m a t h e c a . e r s i c aB A T S C H .( P e r s i c av u l g a r i sM I L L . )PeachType. TypeH o s t : PrunusP L o c a l i t y :S o i l sfromt h eP e a c h o r c h a r d ,Kurume,FukuokaP r e f e c t u r e ,Japan( K y u s h u ) ,K y u s h u H o r t i c u l t u r a lE x p e r i m e n t a lS t a t i o n . C o l l e c t e d :F e b r u a r y ,1 9 6 5 . B i o n o m i c s :f r e q u e n c y ;Female2 3 :Male1 . REFERENCES A l l e n ,M.W.,( 1 9 5 7 ) : Ar e v i e wo ft h eNematodeg e n u sT r i c h o d o r u sw i t hD e s c r i p t i o n so ft e nnews p e c i e s . I ) :3 2-62 Nematologica2( Cobb,N .A . ,( 1 9 1 3 ) : Newnematodeg e n e r afoundi n h a b i t i n gf r e s hw a t e rand n o n b r a c k i s h回 i l s .J o u r . W a s h . ,A c a d . ,3 :432-444 C o lb r a n s ,R.C . ,( 1 9 5 6 ) : S t u d i e so fp l a n tands o i lnematodesI .Twonews p e c i e sfromQ u e e n s l a n d .Qd.J . a g r i c .S c i . ,1 3 :1 2 3 1 2 6 C h r i s t i e ,J .R.andV.G.Peηy,( 1 9 5 1 ) : Ar o o td i s e a s eo fp l a n t sc a u s e d by a nematode o ft h egen 凶 T r i c h o d o r u s .S c i e n c e ,1 1 3:491-493 Hooper,D .J . ,( 1 9 6 2 ) : Threenews p e c i e so fT r i c h o d o r u s(Nematoda:D o r y l a i m o i d e a )ando b s e r v a t i o n s onT .minorC o l b r a n ,1 9 5 6 .Nematologica7( 4 ) :273-280 S e i n h o r s t ,J .W.,( 1 9 5 4 ) : On T r i c h o d o r u s pachydermusn .s p . (Nematoda:E n o p l i d a ) .J o u r .H e l m i n t h . , 2 8 :1 1 1 1 1 4 S i d d i q i i ,M.R . ,( 1 9 6 0 ) : Two news p e c i e so ft h eg e n u sT r i c h o d o r u s(Nematoda: D o r y l a i m o i d e a ) from I n d i aP r o c .Wash.Acad.S c i . ,3 :432-444 S i d d i q i ,M.R . ,( 1 9 6 2 ) : T r i c h o d o r u sp a k i s t a n e n s i sn .s p .(Nematoda:( T r i c h o d o r i d a e ) ,w i t ho b s e r v a t i o n s onT .p o r o s u sA l l e n ,1 9 5 7 ;T .m i r z a iS i d d i q i ,1 9 6 0 ,andT .minorC o l b r a n ,1 9 5 6 ,fromI n d i a . Nematologica8( 3 ) :1 9 3 2 0 0 Yokoo.T . ,( 1 9 6 4 ) : Ont h eStubbyRootNematodesfromt h eWesternJ a p a n .A g r i c ,B u l l .SagaUniv.2 0 : 5 7 6 2 4 佐 賀 大 学 農 学 祭 報 第2 3号 1 f 恥 4 0 . 1 mm 1 . 0 mm 6 7 I lt i f ヘ1 泊 −‘ ...’・、~』~ 5 0 5 I . 2 . 3 . 4 . μ F i g .1 T r i c h o d o r u sk u r u m e e n s i sYOKOO,1 9 6 6 Female 5 . Male:P o s t e r i o rp a r tf oBody Larva 6 . Male O n c h i o s t y l eandA n t e r i o rp a r to fBody 7 . . r 在a l e :S p i c u l a Male:A n t e r i o rp a r to fBody 1 5 TamioYOKOO: OnanewStubbyRootNematode 2 4 3 F i g .2 T r i c h o d o r u sKurumeensisYOKOO,1 9 6 6 I . Femalej u s tb e f o r et h em o u l t i o g 2 . } . 在a l e :a n t e r i o rp a r to fBody 3 . S p i c u l aandT a i lo fMale 4 . Male:p o s t e r i o rp a r to fBody EE ぷ EE − ω 民 、吉岡 2 仏DEO 陥 ~ c < > 5 ・同 国 」 = 吋﹀﹄ dNJ U何 − 日 ﹄omzp o n o 円四 ぴラ 広 ω ロ 自 - ・同 . r : 」 ヨ −− 司 ° " F hwmNM制 帆起 < . O 出時制畑山府組制貯MRM
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