Lentiviral Gene Ontology Vector, LeGO

Lentiviral Gene Ontology Vector, LeGO-G/BSD
Kristoffer Weber, Udo Bartsch, Carol Stocking, Boris Fehse
Molecular Therapy (2008); 16 4 698–706
http://www.LentiGO-Vectors.de
Important sites:
p
Am
CM
V
R
R
U
5
Ps
pUC
i
RRE
cP
PT
3' S I N L T R
LeGO-G/BSD
7871 bp
E
PR
U6
W
XbaI - 2616 - T'CTAG_A
B sd
SF
FV
HpaI - 2935 - GTT'AAC
XhoI - 2950 - C'TCGA_G
eG FP
Cre/loxP - 4687 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
Cre/loxP - 3026 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
EcoRI - 4652 - G'AATT_C
BsrGI - 4641 - T'GTAC_A
BsrGI - 4237 - T'GTAC_A
NotI - 3065 - GC'GGCC_GC
BamHI - 3515 - G'GATC_C
All single and double cutters:
MfeI - 164 - C'AATT_G
MluI - 231 - A'CGCG_T
SpeI - 252 - A'CTAG_T
AseI - 260 - AT'TA_AT
NdeI - 487 - CA'TA_TG
SnaBI - 593 - TAC'GTA
NcoI - 613 - C'CATG_G
KasI - 1018 - G'GCGC_C
CM
NarI - 1019 - GG'CG_CC
V
SfoI - 1020 - GGC'GCC
R
U
BbeI - 1022 - G_GCGC'C
5
BspHI - 7786 - T'CATG_A
SspI - 7755 - AAT'ATT
ScaI - 7431 - AGT'ACT
FspI - 7173 - TGC'GCA
AseI - 7123 - AT'TA_AT
BspHI - 6778 - T'CATG_A
p
Am
R
Ps
i
pUC
FseI - 1531 - GG_CCGG'CC
MfeI - 1572 - C'AATT_G
PT
cP
U6
PR
E
SacII - 5263 - CC_GC'GG
W
PvuII - 5517 - CAG'CTG
KpnI - 5490 - G_GTAC'C
Acc65I - 5486 - G'GTAC_C
RRE
LeGO-G/BSD
7871 bp
3' S I N L T R
BspEI - 5864 - T'CCGG_A
B sd
PciI - 4705 - A'CATG_T
Cre/loxP - 4687 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
EcoRI - 4652 - G'AATT_C
BsrGI - 4641 - T'GTAC_A
PvuII - 4449 - CAG'CTG
BsrGI - 4237 - T'GTAC_A
SF
eG FP
FV
PstI - 2420 - C_TGCA'G
PspOMI - 2608 - G'GGCC_C
ApaI - 2612 - G_GGCC'C
XbaI - 2616 - T'CTAG_A
SspI - 2719 - AAT'ATT
HpaI - 2935 - GTT'AAC
XhoI - 2950 - C'TCGA_G
Cre/loxP - 3026 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
NotI - 3065 - GC'GGCC_GC
NheI - 3100 - G'CTAG_C
BmtI - 3104 - G_CTAG'C
PstI - 3109 - C_TGCA'G
XmaI - 3240 - C'CCGG_G
SmaI - 3242 - CCC'GGG
AscI - 3483 - GG'CGCG_CC
BamHI - 3515 - G'GATC_C
NcoI - 3526 - C'CATG_G

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