CspCI (6195) XmnI (5893) Acc65I (653) CspCI (24) DraIII (230) ScaI (5774) I-S ce p Am 0 300 PacI (1102) B PmeI (1111) PstI (1124) 0 120 51 00 0 90 54 0 BspDI (1084) NdeI (1364) 1500 4800 SnaBI (1470) pBT346 6206bp pro mo te r 1800 CA 21 00 00 42 SapI (4285) ClaI (1084) HindIII (1089) t at 0 0 39 0 at a_ bd lam SacI (4007) tR Eco53kI (4005) SV 1 40 NotI (3871) SpeI (3858) AscI (3849) SwaI (3841) Tth111I (1011) PflFI (1011) PaeR7I (789) PciI (4401) BspQI (4285) TliI (789) lam bd a_ a 4 500 AflIII (4401) XhoI (789) L1 60 0 BmgBI (1003) tt 00 57 AhdI (5294) KpnI (657) I-SceI (675) 36 00 po ly HpaI (3716) MfeI (3703) BsaBI (3615) BclI (3610) EcoRI (3596) 00 24 A 3300 3000 0 270 BlpI (2272) BsiWI (2304) BfuAI (2342) BspMI (2342) GFP4m k Koza SgrAI (2430) BstAPI (2464)
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