Lentiviral Gene Ontology Vector, LeGO

Lentiviral Gene Ontology Vector, LeGO-iT2
Kristoffer Weber, Udo Bartsch, Carol Stocking, Boris Fehse
Molecular Therapy (2008); 16 4 698–706
http://www.LentiGO-Vectors.de
Important sites:
CM
V
pR
Am
R
U5
Ps
pU
C
i
3' SIN LTR
RRE
c PP
T
LeGO-iT2
8540 bp
SF
RE
FV
WP
td T
Cre/loxP - 5356 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
Cre/loxP - 2696 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
IR E
o m a to
S
BamHI - 3189 - G'GATC_C
EcoRI - 3211 - G'AATT_C
NotI - 3247 - GC'GGCC_GC
BsrGI - 5283 - T'GTAC_A
All single and double cutters:
BspHI - 8455 - T'CATG_A
SspI - 8424 - AAT'ATT
ScaI - 8100 - AGT'ACT
PvuI - 7990 - CG_AT'CG
AseI - 7792 - AT'TA_AT
BspHI - 7447 - T'CATG_A
pR
Am
Ps
MfeI - 164 - C'AATT_G
NruI - 211 - TCG'CGA
MluI - 231 - A'CGCG_T
SpeI - 252 - A'CTAG_T
AseI - 260 - AT'TA_AT
NdeI - 487 - CA'TA_TG
SnaBI - 593 - TAC'GTA
CM
V
KasI - 1018 - G'GCGC_C
R
NarI - 1019 - GG'CG_CC
U5
SfoI - 1020 - GGC'GCC
BbeI - 1022 - G_GCGC'C
i
pU
C
NruI - 1214 - TCG'CGA - dam methylated!
3' SIN LTR
LeGO-iT2
8540 bp
Cre/loxP - 2696 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
T
c PP
SF
RE
PciI - 5374 - A'CATG_T
Cre/loxP - 5356 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
td T
o m a to
BsrGI - 5283 - T'GTAC_A
AfeI - 5237 - AGC'GCT
BsiWI - 4803 - C'GTAC_G
AfeI - 4511 - AGC'GCT
PspOMI - 2608 - G'GGCC_C
ApaI - 2612 - G_GGCC'C
NheI - 2774 - G'CTAG_C
BmtI - 2778 - G_CTAG'C
XmaI - 2914 - C'CCGG_G
SmaI - 2916 - CCC'GGG
AscI - 3157 - GG'CGCG_CC
BamHI - 3189 - G'GATC_C
EcoRI - 3211 - G'AATT_C
AscI - 3239 - GG'CGCG_CC
S
IR E
SacII - 3247 - CC_GC'GG
NotI - 3247 - GC'GGCC_GC
SnaBI - 3256 - TAC'GTA
PspOMI - 3389 - G'GGCC_C
ApaI - 3393 - G_GGCC'C
AvrII - 3427 - C'CTAG_G
PmlI - 3592 - CAC'GTG
Acc65I - 3717 - G'GTAC_C
KpnI - 3721 - G_GTAC'C
PciI - 3766 - A'CATG_T
BsiWI - 4077 - C'GTAC G
FV
WP
PvuII - 6186 - CAG'CTG
KpnI - 6159 - G_GTAC'C
Acc65I - 6155 - G'GTAC_C
PvuI - 6038 - CG_AT'CG
SacII - 5932 - CC_GC'GG
RRE
BspEI - 6533 - T'CCGG_A
MfeI - 1572 - C'AATT_G

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