Lentiviral Gene Ontology Vector, LeGO

Lentiviral Gene Ontology Vector, LeGO-G2
Kristoffer Weber, Udo Bartsch, Carol Stocking, Boris Fehse
Molecular Therapy (2008); 16 4 698–706
http://www.LentiGO-Vectors.de
Important sites:
Am
CM
V
pR
R
U
5
Ps
pUC
i
R RE
cP
PT
LTR
3' SIN
LeGO-G2
7137 bp
W
PR
E
SF
FV
Cre/loxP - 2696
NotI - 2735
eGF P
Cre/loxP - 3953
EcoRI - 3918
BamHI - 3185
All single and double cutters:
MfeI - 164 - C'AATT_G
NruI - 211 - TCG'CGA
MluI - 231 - A'CGCG_T
SpeI - 252 - A'CTAG_T
AseI - 260 - AT'TA_AT
NdeI - 487 - CA'TA_TG
SnaBI - 593 - TAC'GTA
NcoI - 613 - C'CATG_G
CM
KasI - 1018 - G'GCGC_C
V
NarI - 1019 - GG'CG_CC
R
SfoI - 1020 - GGC'GCC
BbeI - 1022 - G_GCGC'C
BspHI - 7052 - T'CATG_A
SspI - 7021 - AAT'ATT
ScaI - 6697 - AGT'ACT
PvuI - 6587 - CG_AT'CG
FspI - 6439 - TGC'GCA
AseI - 6389 - AT'TA_AT
Am
BspHI - 6044 - T'CATG_A
pR
U
5
Ps
NruI - 1214 - TCG'CGA - dam methylated!
i
pUC
NgoMIV - 1527 - G'CCGG_C
NaeI - 1529 - GCC'GGC
FseI - 1531 - GG_CCGG'CC
MfeI - 1572 - C'AATT_G
R RE
PT
cP
W
PvuII - 4783 - CAG'CTG
KpnI - 4756 - G_GTAC'C
Acc65I - 4752 - G'GTAC_C
PvuI - 4635 - CG_AT'CG
NaeI - 4540 - GCC'GGC
NgoMIV - 4538 - G'CCGG_C
SacII - 4529 - CC_GC'GG
L TR
3' SIN
BspEI - 5130 - T'CCGG_A
LeGO-G2
7137 bp
PR
E
PciI - 3971 - A'CATG_T
Cre/loxP - 3953 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
EcoRI - 3918 - G'AATT_C
BsrGI - 3907 - T'GTAC_A
SF
eGF P
FV
PstI - 2420 - C_TGCA'G
PspOMI - 2608 - G'GGCC_C
ApaI - 2612 - G_GGCC'C
Cre/loxP - 2696 - ATAACTTCGTATAGCATACATTATACGAAGTTAT
NotI - 2735 - GC'GGCC_GC
NheI - 2770 - G'CTAG_C
BmtI - 2774 - G_CTAG'C
PstI - 2779 - C_TGCA'G
XmaI - 2910 - C'CCGG_G
SmaI - 2912 - CCC'GGG
AscI - 3153 - GG'CGCG_CC
BamHI - 3185 - G'GATC_C
NcoI - 3196 - C'CATG_G

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