Supplementary Table 1. TALEN Characteristics Unique Spacer restriction sites Gene Exon length in spacer Organism Mus musculus Apc 9 16 PvuII 17 15 Mus musculus Î’-catenin 2 16 XmnI 15 15 TALEN size and reqeat variable di-residue sequence, left TALEN shown in upper row NI NN HD NG NI NG NN NG HD HD NI NN HD NG HD HD HD HD HD NI NN NI HD NG NN HD HD NN HD NI NG NN NN NN HD NI NN HD NI NN HD NI NN NG HD NG NG NN NG NN NN NG NN NN NG NN NN HD NI HD HD NI TALEN target sequence (plus strand) T AGCTATGTCCAGCTCCC aagacagctgtatatc CATGCGGCAGTCTGG A T GGCAGCAGCAGTCTT acttggattctggaatccattc TGGTGCCACCACCAC A
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