n 机ite Bー00d Ceーー LeveーS in Rats

人間科学研究
Vo1．18，Supp1ement（2005）
修士論文要旨
E脆cts ofHypokinesia／Hypodynamia andβ2−agon1st
on W五ite B1ood Ce11Leve1s in Rats
Ken SHIRATO
Introduction
Director：Kazuhiko IMAIZUMI
Resu1ts and Discussion
Prolonged hypokinesia，i．e．，decreased motion
1．Tota1white blood ce11s：Tota1white b1ood cen
ac−tivity and／or hypodynamia，i．e．，decreased
numbers in the IMM and WBS groups were1．3−1．9
mechanical loading resu1ting from pro1onged bed
and l．5−1．8times，respective1y，significantly higher
rest，1ife in a wheel chair，restricted movement，joint
than those in the CON groups．No effects，however，
immobilization，1imited muscular function and
were obsemed in the CLE−administration．
space night are known to induce marked1y changes
2．Neutrophi1s：NeutrophiI numbers in the IMM，
in responses of physio1ogica1defense systems such
WBS and CLE−administration groups were2．2−3．8，
as centra1nemous system，endocrine systems and
1．9＿2．9and l．5＿1．6times，respectively，significantIy
immune systems．However，physio1ogica1effects of
higher than those in the CON groups．
hypokinesia／hypodynamia on immune functions
3．Monoc皿es：Monocyte numbers in the IMM，
such as white b1ood ce11s，neutrophi1s，monocytes，
WBS and CLE−administration groups were2．2−3，1，
1ymphocytes，eosinophils and basophi1s are stil1
L8−3．8and l．6times，respectively，significant1y
unknown．Therefore，it is necessary to clarify
higher than those in the CON groups．
systematicauy the physio1ogical responses obtained
4．Lymphocytes：Lymphocyte numbers did not
by hypokinesia／hypodynamia such as restricted
change with the IMM and WBS．The CLE−adminis−
physical activity model and who1e body suspension
tration，however，decreased1ymphocyte numbers to
（weight1essness）modeL In addition，β2−agonist，
0．5＿O．6times，as compared with the CON group．
c1enbutero1（CLE）is known to be useful as
5．Eosinophi1s：Eosinophi1numbers in the IMM
sympathetic activated mode1．Therefore，the ef−
and WBS groups were l．6−2．5and1．5−2．l times，
fects of immobi1ization（IMM），who1e body suspen−
respective1y，significantly higher than those in the
sion（WBS）and CLE−administration on the numbers
CON groups．The CLE−administration，however，
ofwhite blood ce11s were studied in adu1t ma1e rats．
decreased eosinophi1numbers to0．53times，as
compared with the CON group．
Methods and Materia1s
6．Basophi1s：Basophi1numbers in the IMM and
Three experiments，the effects of1）IMM，2）
WBS groups were1．6and2．8−4．8times，re−
WBS and3）CLE−administration on the numbers of
spective1y，higher than those in the CON groups．No
white b1ood ce11s in rats，were carried out．7to g
effects，however，were obsewed in the CLE−
weeks o1d male Sprague Daw1ey rats were used，and
administration．
divided into two groups in each experiment．1）The
IMM was performed for lO days by se1トmade IMM
apparatus．Rats were divided into the IMM group
Conc1usion
The IMM and WBS increased the numbers of nat−
and the control（CON）group．2）The WBS was
ural immune ce1ls（neutrophi1s，monocytes，eosino−
performed for10days according to the routine
method．3）The CLE（dose：1〃8／塘BW／狛γ）was
phils and basophi1s）without changing acquired im−
administered to rats for lO days．Who1e b1ood was
sponses of naturaI immune ce11s were found
co11ected from tai1veins and used for white b1ood
betweenthe IMMandWBS，andCLE−administration．
mune ce11s（lymphocytes）．However，different re−
ce11ana1yses．
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