Identification of minor histocompatibility antigens by reverse

Cover Page
The handle http://hdl.handle.net/1887/28890 holds various files of this Leiden University
dissertation.
Author: Hombrink, Pleun
Title: Identification of minor histocompatibility antigens by reverse immunology
Issue Date: 2014-09-23
Identification of minor histocompatibility
antigens by reverse immunology
Pleun Hombrink
Identification of minor histocompatibility antigens by reverse immunology
© 2014 Pleun Hombrink, Amsterdam
Cover image:
Layout: Printing: Joke Haverkate, 2014, Steenmannetjes
kleurenets, combinatie vernis-mou en aquatint techniek
Liset Westera
Gildeprint, Enschede, The Netherlands
ISBN: xxx-xx-xxx-xxxx-x
The research described in this thesis was financially supported by the Dutch Cancer
Society (grant 07-3825) and the Landsteiner Foundation for Blood Transfusion
Research (grant LSBR0713).
Printing of this thesis was financially supported by the Dutch Cancer Society (KWF)
and eBioscience.
Identification of minor histocompatibility
antigens by reverse immunology
Proefschrift
ter verkrijging van
de graad van doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties
te verdedigen op dinsdag 23 september 2014
klokke 16:15 uur
door
Pleun Hombrink
geboren te Eindhoven
in 1982
Promotiecommissie
Promotor: Prof. dr. J.H.F. Falkenburg
Co-promotor:
Dr. M.H.M. Heemskerk
Overige leden:
Prof. dr. R.A.W. van Lier
Prof. dr. B.O. Roep
Dr. T. Mutis
Contents
Chapter 1
General introduction and Aim of the study
Chapter 2
High-throughput identification of potential minor
histocompatibility antigens by MHC tetramer-based
screening: feasibility and limitations
29
Chapter 3
Mixed functional characteristics correlating with TCR-ligand
koff -rate of MHC-tetramer reactive T-cells within the naïve
T-cell repertoire
73
Chapter 4
Discovery of T-cell epitopes implementing HLA-peptidomics
into a reverse immunology approach
99
Chapter 5
Identification of clinically relevant minor histocompatibility
antigens within the B-lymphocyte derived HLA-ligandome
using a reverse immunology approach
123
Chapter 6
Summary and General discussion
151
7
Appendices Nederlandse samenvatting
167
Dankwoord
179
Curriculum vitae
181
List of publications
182

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