ᒱ㜧┴㎰ᴗᢏ⾡ࢭࣥࢱ࣮◊✲ሗ࿌ ➨ 12 ྕ㸸27㹼33 㸦2012㸧 ኳᩛ࢘ࣝࢫࢆ⏝࠸ࡓࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿᚤ⏕≀㎰⸆ࡢ㛤Ⓨ ⚄㇂ ඞᕭ࣭㕥ᮌ㑳Ꮚ࣭ᯇᑿᑦ࣭Ἑᮧ ᩄ Development of a microbial pesticide for common cutworm, Spodoptera litura by the use of entomopathogenic viruses. Katsumi Kamiya, Ikuko Suzuki, Naonori Matsuo and Satoshi Kawamura せ⣙ ࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿ⏕≀ⓗ㜵㝖㈨ᮦࢆ㛤Ⓨࡍࡿࡓࡵࠊ⮬↛⏺ࡼࡾࣁࢫࣔࣥ ࣚ ࢺ ࢘ ᰾ ከ ゅ య ࢘ ࣝ ࢫ 㸦 SpltNPV㸧 ࢆ ከ ᩘ ศ 㞳 ࡋ ࠊ 㑇 ఏ Ꮚ ゎ ᯒ ཬ ࡧ ⏕ ≀ Ꮫ ⓗ ≉ ᛶ ホ ౯ ࢆ ⾜ ࠺ ࡇ ࡼ ࡾ ࠊ SpltNPV ࡣ 㑇 ఏ ⓗ ࡞ ከ ᵝ ᛶ ࡀ ࠶ ࡾ ࠊ ẅ ຠ ᯝ ࢆ ࡣ ࡌ ࡵ ࡋ ࡓ ≉ ᛶ ࡢ ␗࡞ࡿᵝࠎ࡞࢘ࣝࢫᰴࡀᏑᅾࡋ࡚࠸ࡿࡇࢆ᫂ࡽࡋࡓࠋࡑࡋ࡚ࠊศ㞳ࡋࡓ࢘ࣝࢫ ᰴࡢ୰ࡽཎᛶࡀ㧗ࡃࠊ㜵㝖ຠᯝࡢྥୖࡘ࡞ࡀࡿ 2 ✀㢮ࡢ࢘ࣝࢫᰴࢆ㑅ᢤࡋࠊࡑࢀ ࡽ ࢆ ᭷ ຠ ᡂ ศ ࡋ ࡚ ⏝ ࡋ ࡓ ᚤ ⏕ ≀ ㎰ ⸆ ࠝ SpltNPV 〇 㸦 ၟ ရ ྡ 㸸 ࣁ ࢫ ࣔ ࣥ ࢟ ࣛ ࣮ 㸧ࠞ ࢆ ࠊ ᥬᩫᕝᕤᴗᰴᘧ♫ࡢඹྠ◊✲ࡼࡾ㛤Ⓨࡋࡓࠋᮏ〇࡛⏝ࡋ࡚࠸ࡿ᪂ࡓⓎぢࡋࡓ ࢘ࣝࢫᰴࡢ㸯ࡘࡣࠊᗂࡀឤᰁࡋ࡚ࡽ⮴Ṛࡍࡿࡲ࡛ࡢ㛫ࡀ▷࠸≉ᚩࡀ࠶ࡾࠊࡇࡢ࢘ ࣝࢫᰴẅຊࡀᙉ࠸࢘ࣝࢫᰴࡢ 2 ✀㢮ࡢ࢘ࣝࢫᰴࢆ⤌ࡳྜࢃࡏ࡚⏝࠸ࡿࡇ࡛ࠊ 㧗࠸㜵㝖ຠᯝࡀᚓࡽࢀࡓࠋ ࣮࣮࢟࣡ࢻ㸸ࣁࢫࣔࣥࣚࢺ࢘ࠊᚤ⏕≀㎰⸆ࠊኳᩛ࢘ࣝࢫࠊ᰾ከゅయ࢘ࣝࢫ ⥴ ゝ ࣔࣥࣚࢺ࢘ࡢኳᩛ࢘ࣝࢫ࡛࠶ࡿ SpltNPV ࡣࠊ⣙ 40 ࣁࢫࣔࣥࣚࢺ࢘ࡢᗂࡣࠊ㞧㣗ᛶ࡛ࢲࢬࡸࢳࢦࢆ ᖺ๓ࡽᚤ⏕≀㎰⸆ࡋ࡚ᐇ⏝ࡀ᳨ウࡉࢀ࡚ࡁࡓࡀࠊ ࡣࡌࡵࡋࡓ㔝⳯㢮ࠊⰼ༘㢮࡞ 80 ✀㢮௨ୖࡢᗈ⠊ᅖ Ꮫྜᡂ㎰⸆ẚ㐜ຠᛶ࡛࠶ࡿࡇࡸᏳᐃࡋࡓ㔞 ࡢస≀ࢆຍᐖࡍࡿᐖ࡛ࠊ୰㱋௨㝆ࡢᡂ⫱ࡢ㐍ࢇࡔᗂ ⏕⏘ᢏ⾡ࡀ☜❧ࡉࢀ࡚࠸࡞࠸࡞ࡢၥ㢟Ⅼࡽᐇ⏝ ࡛ࡣẅᑐࡍࡿឤཷᛶࡀపୗࡍࡿࡓࡵ㜵㝖ࡀ㞴ࡋ ࡣ⮳ࡽ࡞ࡗࡓ 1)2)ࠋࡋࡋࠊ㏆ᖺࡢ⎔ቃಖᆺ㎰ᴗ ࠸ࠋ㛵ᮾ௨すࡢᬮᆅ࡛Ⓨ⏕ࡋࠊࡶࡶ⪏ᐮᛶࡀప ᑐࡍࡿ㛵ᚰࡢ㧗ࡲࡾࡼࡾࠊኳᩛ࢘ࣝࢫࡣࡧὀ┠ࡉ ࠸ࡓࡵ㟢ᆅ࡛ࡢ㉺ࡣᅔ㞴࡛࠶ࡗࡓࡀࠊࣁ࢘ࢫ࡞ࡢ ࢀࡘࡘ࠶ࡿࠋᮏ┴࡛ࡣࠕࡂࡩࢡ࣮ࣜࣥ㎰ᴗࠖ㈉⊩ࡍࡿ タෆ࡛ࡣ㉺ྍ⬟࡞ࡓࡵࠊタࡢᬑཬࡶ⿕ᐖࡀቑ ⏕≀ⓗ㜵㝖㈨ᮦࢆ㛤Ⓨࡍࡿ┠ⓗ࡛ࠊࡇࡢ SpltNPV ࢆ⏝ ࡋࡓ⪃࠼ࡽࢀ࡚࠸ࡿࠋᮏ┴ࡢࣁࢫࣔࣥࣚࢺ࢘ࡣࠊ㟢 ࠸ࡓࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿ᪂ࡋ࠸ᚤ⏕≀㎰⸆ࡢ㛤Ⓨ ᆅ࡛ࡣ 7 ᭶ୗ᪪ࡽ 11 ᭶ึ᪪ࡅ࡚Ⓨ⏕ࡋࠊ≉ 8 ࢆ㐍ࡵࠊ ࠕࣁࢫ࣮ࣔࣥ࢟ࣛࠖ࠸࠺ၟရྡ࡛ᥬᩫᕝᕤᴗ ᭶ࡽ 9 ᭶ࡅ࡚Ⓨ⏕ࡀ᭱ࡶከࡃ࡞ࡾࠊ⏒࡞⿕ᐖࢆ ᰴᘧ♫㸦ᮏ♫ࠊᒱ㜧┴ᇉᕷ㸧ࡀ㎰⸆Ⓩ㘓ࢆ⏦ㄳࡋࠊ ࡶࡓࡽࡍࡇࡀ࠶ࡿࠋࡲࡓࠊࣁ࢘ࢫ᱂ᇵ࡛ࡣ 11 ᭶ୗ᪪ ᖹᡂ 24 ᖺ 4 ᭶ࢆ┠ฎⓏ㘓ㄆྍࡉࢀࡿணᐃ࡛࠶ࡿࠋ ࡲ࡛⿕ᐖࡀㄆࡵࡽࢀࡿࠋ ᭱㏆10ᖺ㛫࠾ࡅࡿⓎ⏕㔞ࡣࠊ ᮏሗ࿌࡛ࡣࠊࣁࢫ࣮ࣔࣥ࢟ࣛࡢ㛤Ⓨ࠶ࡓࡾࠊ⮬↛⏺ ᖺࡼࡿࣂࣛࢶ࢟ࡣ࠶ࡿࡀࠊ≉ᖹᡂ 16 ᖺ࠾ࡼࡧ 22 ᖺ ࡼࡾศ㞳ࡋࡓ SpltNPV ᰴࡢ୰ࡽ㜵㝖ຠᯝࡢ㧗࠸ᰴࢆ ࡣࠊⓎ⏕ணᐹὀពሗࡀⓎ௧ࡉࢀࡿ࡞ࡁ࡞⿕ᐖࢆཷࡅ 㑅ᢤࡋ࡚ᚤ⏕≀㎰⸆ࡋ࡚ᐇ⏝ࡍࡿࡲ࡛ࡢ⤒㐣ࠊᅡ ࡓࠋࣁࢫࣔࣥࣚࢺ࢘ࡣࠊᱵ㞵᫂ࡅᚋࡢኳೃࡀ㧗 ᑡ㞵࡛ ሙ࡛ࡢ㜵㝖ຠᯝ࠾ࡼࡧ㜵㝖ຠᯝᙳ㡪ࢆ࠼ࡿせᅉࡢ ࠶ࡿࡇࡀⓎ⏕ࡢዲ㐺᮲௳⪃࠼ࡽࢀ࡚࠾ࡾࠊࡢ ゎᯒࡘ࠸࡚ሗ࿌ࡍࡿࠋ ᬮࡢᙳ㡪ࡶཷࡅࠊⓎ⏕ࡀከࡃ࡞ࡿணࡉࢀࡿࠋ ᰾ከゅయ࢘ࣝࢫ㸦NPV㸧ࡣࠊࣂ࢟ࣗࣟ࢘ࣝࢫ⛉ 1㸬⮬↛⏺ࡽࡢ SpltNPV ࡢศ㞳㑇ఏⓗศ㢮 ᒓࡍࡿ᪻ᅛ᭷ࡢ࢘ࣝࢫ࡛࠶ࡾࠊ࢘ࣝࢫ⢏Ꮚࡀከ [┠ⓗ] ᩘໟᇙࡉࢀ࡚࠸ࡿከゅయࡤࢀࡿ⢏Ꮚࢆᙧᡂࡋࠊከゅ ⮬↛⏺Ꮡᅾࡍࡿ SpltNPV ࢆศ㞳ࡋ࡚ࠊ㑇ఏⓗ࡞ከ యࢆ᳜≀ࡶᗂࡀᦤ㣗ࡍࡿࡇ࡛ឤᰁࡍࡿࠋࣁࢫ ᵝᛶࢆ᫂ࡽࡍࡿࠋ ኳᩛ࢘ࣝࢫࢆ⏝࠸ࡓࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿᚤ⏕≀㎰⸆ࡢ㛤Ⓨ 2㸬SpltNPV ࢡ࣮ࣟࣥࡢ㑅ᢤ⏝ἲࡢ㛤Ⓨ [ᮦᩱ࠾ࡼࡧ᪉ἲ] ⮬↛⏺ࡢ᰾ከゅయ࢘ࣝࢫ⨯ᗂࡣ㑇ఏⓗ ␗࡞ࡿࢡ࣮ࣟࣥࡀΰᅾࡋࠊẅຊࡶᕪࡀ࠶ࡿࡇࡀ᫂ (1) SpltNPV ࢡ࣮ࣟࣥࡢẅ≉ᛶࡢẚ㍑ [┠ⓗ] 4)ࠋࡑࡇ࡛ࠊ᪥ᮏྛᆅ࡛᥇㞟࣭ಖᏑࡉ SpltNPV 㑇ఏᏊࢱࣉࡑࢀࡒࢀࡢẅ≉ᛶࢆ᫂ࡽ ࢀ࡚࠸ࡓᰴࡢศㆡࢆཷࡅࡿࡶࠊ᪂ࡓ㔝እ⨯ ࡍࡿࡇࡼࡾඃⰋࢡ࣮ࣟࣥࢆ㑅ᢤࡋࠊᚤ⏕≀㎰⸆ ࡢ᥇㞟ࢆ⾜࠸ࠊࡑࢀࡒࢀࡘ࠸࡚ᇵ㣴⣽⬊ࢆ⏝࠸ࡓࣉࣛ ࡋ࡚᭱㐺࡞ࢡ࣮ࣟࣥࢆỴᐃࡍࡿࠋ ࡽ࡞ࡗ࡚࠸ࡿ ࣮ࢡ⣧ࡼࡾ㑇ఏⓗ⣧⢋࡞࢘ࣝࢫᰴ㸦ࢡ࣮ࣟࣥ㸧 [ᮦᩱ࠾ࡼࡧ᪉ἲ] ࡢศ㞳ࢆ⾜ࡗࡓࠋ㔝እ⨯⏤᮶ࢧࣥࣉࣝࡽྜィ 189 㑇ఏⓗ࡞ከᵝᛶࡢ୰ࡽẅຊࡢᙉ࠸ࢡ࣮ࣟࣥࡢࡳ ࢡ࣮ࣟࣥࢆศ㞳ࡋࠊྛࢡ࣮ࣟࣥࡢࢤࣀ࣒ DNA ࢆᢳฟࡋ ࢆ㑅ᢤࡋ࡚⏝ࡍࡿࡇࡼࡾ㜵㝖ຠᯝࡸ㔞⏕⏘ ࡚ࠊྛ✀ไ㝈㓝⣲ࡼࡗ࡚ษ᩿ࡉࢀࡓࣇࣛࢢ࣓ࣥࢺࡢࣃ ࡢ㑇ఏⓗ࡞Ᏻᐃᛶࡀ㧗ࡲࡿ⪃࠼ࡽࢀࡿࠋࡑࡇ࡛ࠊྛࢱ ࢱ࣮ࣥࢆẚ㍑ࡋࡓࠋ ࣉࡢ௦⾲ᰴ2 ᰴࡘ࠸࡚ࣁࢫࣔࣥࣚࢺ࢘3 㱋ᗂࢆ⏝ [⤖ᯝ] ࠸ࡓ⏕≀᳨ᐃࡼࡾẅຊࢆẚ㍑ࡋࡓࠋࢻࣟࢵࣉࣞࢵࢺ ศ㞳ࡋࡓࢡ࣮ࣟࣥࡣࠊࢤࣀ࣒ DNA ࡢไ㝈㓝⣲ࡼࡿ ࣇ࣮ࢹࣥࢢἲࡼࡾࠊ⃰ᗘࡢ␗࡞ࡿከゅయᾮࢆᗂ ษ᩿ࡢ㢮ఝᛶࡽࠊࡁࡃ 3 ࡘࡢࢢ࣮ࣝࣉ㸦AࠊBࠊC 㣧ୗࡉࡏࡓࠋヨ㦂ࡣ 3 㐃ไ࡛⾜࠸ࠊឤᰁᚋ 14 ᪥㛫ࡢ ࢱࣉ㸧ศ㢮ྍ⬟࡛࠶ࡗࡓࠋA ࢱࣉࡣࣁࢫࣔࣥࣚࢺ Ṛஸ⋡ࡽࠊ༙ᩘ⮴Ṛ⃰ᗘ㸦LC50㸧ࢆ⟬ฟࡋࡓࠋࡲࡓࠊ ࢘ࡢ㏆⦕✀࡛࠶ࡿ Spodoptera littoralis ࡽศ㞳ࡉࢀࡓ 1 108 ከゅయ / ml ࡢ⃰ᗘ࡛ឤᰁࡉࡏࠊṚஸ⋡ࢆẖ᪥ㄪ ࠊ ᰾ከゅయ࢘ࣝࢫ㸦NPV㸧ࡢ୍✀㢮ఝࡋ 5) 㸦ᅗ 1㸧 ᰝࡋࠊ༙ᩘ⮴Ṛ㛫㸦LT50㸧ࢆ⟬ฟࡋࡓࠋ B ࢱࣉࡣᅜෆእ࡛ࡇࢀࡲ࡛ศ㞳࣭ሗ࿌ࡉࢀ࡚࠸ࡿ [⤖ᯝ] NPV ୍⮴ࡋࡓ 6)ࠋࡲࡓࠊC ࢱࣉࡣࠊᒱ㜧┴ෆ࡛᪂ࡓ LC50 ್ࡽࠊẅຊࡣࢱࣉ㛫࡛ࡁࡃ␗࡞ࡗ࡚࠾ࡾࠊ ᥇㞟ࡋࡓ⨯ࡽࡢࡳศ㞳࡛ࡁࡓࢱࣉ࡛ࠊࡇࢀࡲ B ࢱࣉࡀ᭱ࡶᙅࡃࠊࡘ࠸࡛ C ࢱࣉࠊA ࢱࣉࡣ᭱ࡶ ࡛ሗ࿌ࡉࢀࡓ NPV ࡣࡲࡗࡓࡃ㢮ఝᛶࡢ࡞࠸᪂つ࡞ ᙉࡗࡓ㸦⾲ 1㸧 ࠋ୍᪉ࠊ LT50 ್㛵ࡋ࡚ࡣࠊC ࢱࣉ ࣃࢱ࣮ࣥࢆ♧ࡋࡓ㸦ᅗ 1㸧 ࠋ ࡢࢡ࣮ࣟࣥࡀ⮴Ṛࡲ࡛ࡢ㛫ࡀ᭱ࡶ▷ࡗࡓ 3)ࠋA ࠾ࡼ (15.4) A (11.8) B 10.6) E CD (11.0, (9.8) G F (8.6)(9.4) H (6.3) I (5.5) J (5.4) K (5.3) L (4.7) M N (4.5)(4.6) O (4.4) P (4.0) Q R ࡧ B ࢱࣉࡣ᥋✀ᚋ 6ࠥ9 ᪥┠ࡅ࡚Ṛஸࡍࡿࡢᑐ &ࢱࣉ C3 (3.4) S (2.2) T (2.0) U (1.8) ࡋ࡚ࠊC ࢱࣉࡣ᥋✀ᚋ 3 ᪥┠ࡽṚஸಶయࡀฟ⌧ࡋࠊ A B C D E F GH I J K (19.6) (13.7) (13.0) LM N (4.7, 4.7) (4.4) O (4.0) PQ (3.6, 3.6) R (3.2) S T (2.7, 2.7) U (2.3) V (2.1) W (1.9) 7 ᪥┠ࡲ࡛ឤᰁࡢࢇࡀṚஸࡋࡓ㸦ᅗ 2㸧 ࠋ௨ୖ (8.6, 8.6) (7.7, 7.7) (7.0, 7.0) (6.4) V (1.7) W (1.6) X (1.5) X Y (1.4) (1.3) Y (1.3) Z (1.1) Z (1.2) a (1.0) ࡢࡇࡽࠊA ࢱࣉࡣẅຊࡀᙉ࠸ᙉẅᛶࡢ≉㛗ࢆ ᭷ࡋࠊC ࢱࣉࡣ⮴Ṛ㛫ࡀ▷࠸ࡓࡵ㐜ຠᛶࢆᨵၿྍ⬟ ࡞≉ᛶࢆ᭷ࡍࡿࡇࡀ᫂ࡽ࡞ࡗࡓࠋࡑࡇ࡛ࠊ㸿ࢱ ࣉࡢࢡ࣮ࣟࣥ C ࢱࣉࡢࢡ࣮ࣟࣥࢆΰྜࡋ࡚⏝࠸ࡿ ᪂つࡢᚤ⏕≀㎰⸆ࢆ⪃ࡋࠊ≉チࢆྲྀᚓࡋࡓ㸦≉チ➨ 4109146 ྕ㸧 ࠋ ᣓᘼෆࡢᩘᏐࡣศᏊ㔞NES ᅗ 6SOW139 ࢡ࣮ࣟࣥࢤࣀ࣒ '1$ ࡢ㓝⣲ษ᩿ࣃࢱ࣮ࣥ ⾲㻝䚷㻿㼜㼘㼠㻺㻼㼂䜽䝻䞊䞁䛾ẅຊ䛾ẚ㍑ 䜽䝻䞊䞁 㻭㻝 㻸㻯㻡㻜 㻌㻔ከゅయ㻌㻛㻌㼙㼘㻕 㻣㻜㻘㻞㻜㻜 㻥㻡㻑ಙ㢗㝈⏺ ୗ್ ୖ್ 㻟㻢㻘㻠㻜㻜 㻝㻞㻜㻘㻜㻜㻜 㻭㻥 㻠㻟㻘㻣㻜㻜 㻞㻞㻘㻠㻜㻜 㻣㻡㻘㻢㻜㻜 㻮㻝 㻝㻘㻥㻠㻜㻘㻜㻜㻜 㻝㻘㻠㻜㻜㻘㻜㻜㻜 㻞㻘㻣㻡㻜㻘㻜㻜㻜 㻮㻥 㻝㻘㻤㻜㻜㻘㻜㻜㻜 㻝㻘㻜㻤㻜㻘㻜㻜㻜 㻟㻘㻠㻞㻜㻘㻜㻜㻜 㻯㻟 㻣㻟㻞㻘㻜㻜㻜 㻟㻠㻟㻘㻜㻜㻜 㻝㻘㻟㻢㻜㻘㻜㻜㻜 ⣼✚Ṛஸ⋡䠄䠂䠅 $ࢱࣉ A9 100 90 80 70 60 50 40 30 20 10 0 1 2 3 4 5 6 7 8 ᥋✀ᚋ᪥䠄᪥䠅 A䝍䜲䝥 B䝍䜲䝥 9 10 11 C䝍䜲䝥 ᅗ 6SOW139 ࢡ࣮ࣟࣥࡢࢱࣉูẅ≉ᛶࡢẚ㍑ ⣼✚Ṛஸ⋡䠄䠂䠅 ᒱ㜧┴㎰ᴗᢏ⾡ࢭࣥࢱ࣮◊✲ሗ࿌ ➨ 12 ྕ㸸27㹼33 㸦2012㸧 ⾲㻞䚷䝎䜲䝈ᅡሙ䛻䛚䛡䜛㜵㝖ຠᯝ 60 ᩓᕸ᪉ἲ 40 㻡᪥ᚋ 㻢᪥ᚋ ᬑ㏻ᩓᕸ 㻣㻥㻚㻢 㻟㻢㻚㻥 㻞㻝㻚㻠 㻤㻚㻞 㧗⃰ᗘᑡ㔞ᩓᕸ 㻠㻤㻚㻠 㻞㻝㻚㻟 㻝㻣㻚㻥 㻝㻝㻚㻟 20 0 5 6 7 9 12 14 ⿵ṇᐦᗘᣦᩘ㻖 㻠᪥ᚋ 㻣᪥ᚋ 㻖䠖↓ฎ⌮༊䛾ᗂᐦᗘ䜢㻝㻜㻜䛸䛧䛯┦ᑐ್ ᥋✀ᚋ᪥䠄᪥䠅 C䝍䜲䝥䛾ྜ 0% 10% 20% 50% 100% ⏝࠸࡚ࠊ1 ༊ 15m2 ࡢ 3 㐃ไ࡛ヨ㦂ࢆᐇࡋࡓࠋ㔝እ᥇ 㞟 1 㱋ࣁࢫࣔࣥࣚࢺ࢘ᗂࢆࠊ8 ᭶ 18 ᪥ྛヨ㦂༊ෆ ᅗ ✀ࢡ࣮ࣟࣥࡢΰྜẚ⋡ࡼࡿẅ≉ᛶࡢẚ㍑ タᐃࡋࡓㄪᰝᰴ 10 ᰴࡢ୰ኸ㒊ศᨺࡋ࡚ࠊ2 ᪥ᚋ ⸆ᩓᕸࡋࡓࠋࣂࢵࢸ࣮ࣜᘧ⫼㈇ືᄇࡼࡾヨస〇 (2) 2 ࢡ࣮ࣟࣥΰྜຠᯝࡢ᳨ウ 1,000 ಸᕼ㔘ᾮ㸦㏻ᖖᩓᕸ㸧ࢆࠊ࢚ࣥࢪࣥᘧ⫼㈇࠸ືᄇ [┠ⓗ] ᩓᕸࣀࢬࣝࡋ࡚ࣃࣥࢡࣝࢫࣉ࣮ࣞࣀࢬࣝࢆ⏝࠸࡚ AࢱࣉCࢱࣉࢆΰྜࡍࡿࡇࡼࡿẅຠᯝࢆ 125 ಸᾮ㸦㧗⃰ᗘᑡ㔞ᩓᕸ㸧ࢆࡑࢀࡒࢀ 10a ࠶ࡓࡾ 200L 㔞ᩓᕸࡋࡓࠋ ᫂ࡽࡍࡿࠋ [⤖ᯝ] [ᮦᩱ࠾ࡼࡧ᪉ἲ] ⴥ㛗⣙ 15cm ࡢࢲࢬⴥ㸦ࣇࢡࣘࢱ࢝ࠊ✀ 55 ᪥ᚋ㸧 ࢆ᥇㞟ࡋࠊࣁࢧ࣑࡛༙ศษࡾከゅయ⁐ᾮᾐₕࡋỈ ⿵ṇᐦᗘᣦᩘࡣࠊᩓᕸฎ⌮ 5 ᪥ᚋࡽᛴ⃭పୗࡋࠊ 7 ᪥ᚋࡣᬑ㏻ᩓᕸ༊࡛ 8.2ࠊ㧗⃰ᗘᑡ㔞ᩓᕸ༊࡛ 11.3 108 ከゅ ࡞ࡾࠊ㧗࠸㜵㝖ຠᯝࡀㄆࡵࡽࢀࡓ㸦⾲ 2㸧 ࠋᬑ㏻ᩓᕸ య / ml ࡢ⃰ᗘ࡛ࠊC ࢱࣉࡢྜࢆ 0ࠊ10ࠊ20ࠊ50 ࠾ ẚ㧗⃰ᗘᑡ㔞ᩓᕸ࡛㜵㝖ຠᯝࡀ᪩ࡃ⌧ࢀࡓࠋᩓᕸ 2 ᪥ ࡼࡧ 100㸣ࡢ 5 ẁ㝵ㄪ〇ࡋࡓࠋᒎ╔ࡋ࡚ࠊࣉࣟ ᚋྛヨ㦂༊ࡽ᥇㞟ࡋࡓᗂࢆᐊෆ࡛㣫⫱ࡋࡓࡇ ࣮ࢳࢆ 1,000 ಸ⃰ᗘ࡛ຍ⏝ࡋࡓࠋ┤ᚄ 60mm ࡢࣉࣛࢫ ࢁࠊ90㸣௨ୖࡢṚஸ⋡ࡀ☜ㄆࡉࢀࡓࠋヨ㦂ᮇ㛫୰ࡢᖹᆒ ࢳࢵࢡ࢝ࢵࣉࢲࢬⴥ 1 ∦ࢆධࢀࠊᐊෆ⣼௦㣫⫱ࡋࡓ Ẽ ࡣࠊᴫࡡ 25Υ௨ୖ࡛᥎⛣ࡋࡓࠋ ࡀ↓ࡃ࡞ࡿࡲ࡛㢼ࡋࡓࠋከゅయ⁐ᾮࡣࠊ1 3 㱋ࣁࢫࣔࣥࣚࢺ࢘ᗂࢆ 1 ༉ධࢀࡓࠋྛ⃰ᗘ 60 ༉ࡢ ᗂࢆ⏝࠸ࡓࠋ25Υࠊ16L-8D ᮲௳࡛ 2 ᪥㛫ᦤ㣗ࡉࡏࡓ 4㸬ᚤ⏕≀㎰⸆ࡢ㜵㝖ຠᯝᙳ㡪ࢆ࠼ࡿせᅉゎᯒ ᚋࠊΎί࡞ேᕤ㣫ᩱ㸦ࣥࢭࢡࢱ LFS㸧ࡋࠊࡑࡢ (1) ᗂ㱋ᮇࡼࡿឤཷᛶࡢẚ㍑ ᚋࡢṚஸ⋡ࢆฎ⌮ᚋ 14 ᪥ᚋࡲ࡛ㄪᰝࡋࡓࠋ [┠ⓗ] [⤖ᯝ] ฎ⌮ᚋ 7 ᪥┠ࡲ࡛ࡣࠊA ࢱࣉ༢⊂ẚ C ࢱࣉࢆ ࣁࢫࣔࣥࣚࢺ࢘ࡢⓎ⫱㱋ࡼࡿ〇ᑐࡍࡿឤཷᛶ ࡢ㐪࠸ࢆ᫂ࡽࡍࡿࠋ ຍ࠼ࡿࡇ࡛᪩ࡃṚஸࡍࡿಶయࡀฟ⌧ࡋࡓ㸦ᅗ 3㸧 ࠋ᭱⤊ [ᮦᩱ࠾ࡼࡧ᪉ἲ] ⓗ࡞Ṛஸ⋡ࡣࠊC ࢱࣉ༢⊂ẚ A ࢱࣉࡀ 10%௨ ᐊෆ⣼௦㣫⫱ࡋࡓࣁࢫࣔࣥࣚࢺ࢘ᗂࢆ⏝࠸ࡓࠋឤᰁ ୖᏑᅾࡋ࡚࠸ࡿሙྜ࡛Ṛஸ⋡ࡀ㧗ࡲࡗࡓࠋ௨ୖࡢ⤖ᯝ ࡣࠊ㣫ᩱΰධἲࡼࡾ⾜ࡗࡓࠋேᕤ㣫ᩱ A ࢱࣉ C ࡽࠊA ࢱࣉ C ࢱࣉࡢΰྜࡼࡾẅᛶ⬟ࡀ㧗ࡲࡿ ࢱࣉࡢΰྜࡋࡓヨస〇㸦ΰྜẚ㠀බ㛤㸧ࢆ 1 108 ࡇࡀ᫂ࡽ࡞ࡗࡓࠋ2 ࢱࣉࡢΰྜࡼࡿᝏᙳ㡪ࡣ ከゅయ / g 㣫ᩱࡢ⃰ᗘ࡛ΰධࡋࠊ25Υࠊ16L-8D ᮲௳࡛ ㄆࡵࡽࢀ࡞ࡗࡓࠋ ྛ㱋 60 ༉௨ୖࡢᗂ 2 ᪥㛫ᦤ㣗ࡉࡏࡓࠋࡑࡢᚋࠊΎ ί࡞ேᕤ㣫ᩱࢆ࠼࡚ࣉࣛࢫࢳࢵࢡ࢝ࢵࣉෆ࡛ಶู㣫 3㸬ࢲࢬᅡሙ࠾ࡅࡿ㜵㝖ຠᯝ [┠ⓗ] ࢲࢬᅡሙ࠾ࡅࡿࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿ㜵㝖ຠ ⫱ࡋ࡚Ṛஸ⋡ࢆẖ᪥ㄪᰝࡋࡓࠋ [⤖ᯝ] ᗂࡢⓎ⫱ࡀ㐍ࡴࡘࢀ࡚ឤཷᛶࡣపୗࡋࡓ㸦ᅗ 4㸧 ࠋ ᯝࢆ᫂ࡽࡍࡿࠋࡲࡓࠊ㧗⃰ᗘᑡ㔞ᩓᕸࡼࡾࠊ࢘ 25Υ᮲௳ୗ࡛ࡣࠊ1 㱋 2 㱋ᗂࡣࡑࢀࡒࢀฎ⌮ᚋ 4ࠊ5 ࣝࢫࡢṧຠᛶࡀ㧗ࡲࡾࠊ㜵㝖ຠᯝࢆᨵⰋ࡛ࡁࡿࢆ☜ㄆ ᪥┠ࡽṚஸࡀฟ⌧ࡋࠊ6 ᪥┠ࡲ࡛ 90%௨ୖࡢṚஸ ࡍࡿࠋ ⋡ࡀᚓࡽࢀࡓࠋ3 㱋࠾ࡼࡧ㸲㱋ᗂࡣࠊ6 ᪥┠ࡽຠᯝ [ᮦᩱ࠾ࡼࡧ᪉ἲ] ࡀ⌧ࢀࠊ80%௨ୖࡢṚஸ⋡ࡀᚓࡽࢀࡿࡣ⣙ 9 ᪥㛫せࡋ ㎰ᴗᢏ⾡ࢭࣥࢱ࣮ෆࡢࢲࢬᅡሙ㸦ရ✀㸸ࣇࢡࣘࢱ࢝ࠊ ࡓࠋ ✀㸸2004 ᖺ 7 ᭶ 12 ᪥ࠊ᮲㛫 75cmᰴ㛫 15cm㸧ࢆ ኳᩛ࢘ࣝࢫࢆ⏝࠸ࡓࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿᚤ⏕≀㎰⸆ࡢ㛤Ⓨ 㻝㻜㻜 㻝㻜㻜 㻤㻜 㻢㻜 ⣼✚Ṛஸ⋡䠄䠂䠅 ⣼✚Ṛஸ⋡䠄䠂䠅 㻤㻜 㻠㻜 㻞㻜 㻢㻜 㻠㻜 㻞㻜 㻜 㻝 㻞 㻟 㻠 㻡 㻢 㻣 㻤 㻥 㻝㻜 㻝㻝 㻝㻞 㻝㻟 㻝㻠 ᥋✀ᚋ᪥䠄᪥䠅 䠍㱋 䠎㱋 䠏㱋 䠐㱋 䠑㱋 䠒㱋 㻜 㻝 㻟 㻡 ᅗ ᗂ㱋ูࡢẅຠᯝ 㻣 㻥 㻝㻝 㻝㻟 㻝㻡 㻝㻣 㻝㻥 㻞㻝 㻞㻟 ᥋✀ᚋ᪥䠄᪥䠅 㻝㻣䉝 㻞㻜䉝 㻞㻡䉝 㻟㻜䉝 㻟㻡䉝 䠎㱋䠍䠓䉝 (2) ᗘࡼࡿẅຠᯝࡢ㐪࠸ [┠ⓗ] ᅗ ࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿ ᗘูẅຠᯝ ᗘࡼࡿ〇ࡢẅຠᯝࡢ㐪࠸ࢆ᫂ࡽࡍࡿࠋ [ᮦᩱ࠾ࡼࡧ᪉ἲ] ⣙ 45cm㸧 ࠊ୰ⴥ㸦ᰴඖࡽ⣙ 30cm㸧ࡲࡓࡣୗⴥ㸦ᰴ ᐊෆ⣼௦㣫⫱ࡋࡓ2 㱋࠾ࡼࡧ3 㱋ࣁࢫࣔࣥࣚࢺ࢘ᗂ ඖࡽ⣙ 15cm ࡲ࡛㸧ศࡅ࡚᥇ྲྀࡋࠊ༙ศษ᩿ࡋࡓ ࢆ⏝࠸ࡓࠋឤᰁࡣࠊA ࢱࣉ C ࢱࣉࡢΰྜ〇ࢆ 5 ࢲࢬⴥࢆࣉࣛࢫࢳࢵࢡ࢝ࢵࣉෆ࡛3 㱋ᗂ1 ༉2 ᪥ 107 ከゅయ 㛫ಶู࡛㣗ୗࡉࡏࡓᚋࠊேᕤ㣫ᩱࢆ࠼࡚ 14 ᪥㛫㣫⫱ / g 㣫ᩱࡢ⃰ᗘ࡛๓㏙ࡢ㣫ᩱΰධἲࡼࡾ ⾜ࡗࡓࠋ 〇ࢆΰධࡋࡓேᕤ㣫ᩱࢆ2᪥㛫ᦤ㣗ࡉࡏࡓᚋࠊ ࡋࠊṚஸ⋡ࢆㄪᰝࡋࡓࠋྠᵝࡢឤᰁ᪉ἲࡼࡾࠊᩓᕸᚋ 17ࠊ20ࠊ25ࠊ30 ࠾ࡼࡧ 35Υࡢྛ ᗘ᮲௳ୗ࡛Ύί࡞㣫 3ࠊ5ࠊ7ࠊ10 ࠾ࡼࡧ 20 ᪥┠ヨ㦂ᅡሙࡽ᥇㞟ࡋࡓࢲ ᩱࢆ࠼࡚ಶู㣫⫱ࡋࠊṚஸ⋡ࢆẖ᪥ㄪᰝࡋࡓࠋ ࢬⴥࡘ࠸࡚ẅάᛶࢆㄪᰝࡋࡓࠋ [⤖ᯝ] ㎰ᴗᢏ⾡ࢭࣥࢱ࣮ෆࢲࢬᅡሙ࠾࠸࡚ࠊ2005 ᖺ 8 ᗘࡀ㧗࠸ᗂࡢ⮴Ṛせࡍࡿ㛫ࡀ▷ࡃ㸪᭱⤊ ᭶ 26 ᪥༗๓ 11 ࢲࢬ㸦ࣇࢡࣘࢱ࢝ࠊ7 ᭶ 19 ᪥ ⓗ࡞Ṛ⋡ࡣ㧗ࡃ࡞ࡾࠊຠᯝࡀ㧗ࡗࡓ㸦ᅗ 5㸧 ࠋ20Υ ✀ࠊⲡ⣙ 40cm㸧ヨస〇 1,000 ಸᕼ㔘ᾮࡢᩓᕸࢆ ௨ୗࡢ ᗘ࡛ࡣᗂࡢ⮴Ṛせࡍࡿ㛫ࡀ㛗ࡃ㸪17Υ᮲ ⾜ࡗࡓࠋኴ㝧ග⥺ࢆཷࡅ࡚࠸ࡿ㡬㒊ⴥࢆᩘ㛫࠾ࡁ᥇ ௳࡛ࡣࠊ 24 ᪥ᚋ⏕Ꮡࡋ࡚࠸ࡓᗂࡣࡍ࡚⻈ࡋࡓࡀ ྲྀࡋࠊୖ㏙᪉ἲࡼࡾࣁࢫࣔࣥࣚࢺ࢘ᗂឤᰁฎ⌮ࢆ Ṛஸ⋡ࡣ 20%ࡲࡗࡓࠋ୍᪉ࠊ2 㱋ᗂ࡛࠶ࢀࡤࠊ ⾜ࡗࡓᚋࠊ14 ᪥㛫ࡢṚஸ⋡ࢆㄪᰝࡋࡓࠋࡲࡓࠊᩓᕸᚋ 6 7 ᪥ᚋࡽຠᯝࡀ⌧ࢀࠊ17 ᪥ᚋࡣ⣼✚Ṛஸ⋡ࡀ 100% 㛫ࠊ1 ᪥┠ࠊ2 ᪥┠ 2 㱋࠾ࡼࡧ 3 㱋ᗂࢆ᥇㞟ࡋ࡚ࠊ 㐩ࡋࡓࡇࡽࠊప ᮲௳ୗ࡛༑ศ࡞ຠᯝࢆᚓࡿࡣࠊ ᐊෆ࡚ேᕤ㣫ᩱࢆ࠼࡚Ṛஸ⋡ࢆㄪᰝࡋࡓࠋ 2 㱋ࡲ࡛ฎ⌮ࡍࡿࡢࡀຠᯝⓗ࡛࠶ࡿ⪃࠼ࡽࢀࡓࠋ [⤖ᯝ] 〇ᩓᕸ3᪥ᚋࡲ࡛ࢲࢬᰴయ࡛ẅάᛶࡀపୗ (3) ኴ㝧ග⥺ࡀṧຠᛶ࠼ࡿᙳ㡪 ࡋࡓ㸦⾲ 3㸧 ࠋࡋࡋࠊ3 ᪥┠௨㝆ࡢάᛶࡢపୗࡣ⦆ࡸ [┠ⓗ] ࡛ࠊ୰ⴥ௨ୗ࡛ࡣ 20 ᪥ᚋ࡛ࡶ 20㸣⛬ᗘࡢṚஸ⋡ࡀᚓ NPV ࡣ⣸እ⥺᭚ࡉࢀࡿࡇࡼࡾάᛶࡀపୗࡍࡿ ࡽࢀࡿ࢘ࣝࢫࡀ⏕ṧࡋ࡚࠸ࡓࠋᩓᕸ᪉ἲࡼࡿṧຠᛶ ࡇࡀศࡗ࡚࠸ࡿࡓࡵࠊヨస〇ࡢᩓᕸᚋࡢṧຠᛶࢆ ࡢ㐪࠸ࡣㄆࡵࡽࢀ࡞ࡗࡓࠋ ᫂ࡽࡍࡿࠋ ࢲࢬᰴ㡬㒊ⴥ࡛ࡣኴ㝧ග⥺ࡢᙳ㡪ࢆཷࡅࠊ2 㛫ᚋ [ᮦᩱ࠾ࡼࡧ᪉ἲ] ࡽẅຊࡀపୗࡋጞࡵࠊ6 㛫ᚋࡣṚஸ⋡ࡀ༙ῶࡋࡓ ㎰ᴗᢏ⾡ࢭࣥࢱ࣮ෆࢲࢬᅡሙ࠾࠸࡚ࠊ2004 ᖺ 8 ᥇㞟ࡋࡓᗂࡣࠊ ᩓᕸᚋ 6 㛫ࡢᦤ㣗࡛ࠊ 㸦ᅗ 6㸧 ࠋ ୍᪉ࠊ ᭶ 20 ᪥ṇ༗ࢲࢬ㸦ࣇࢡࣘࢱ࢝ࠊ7 ᭶ 12 ᪥✀ࠊⲡ 3 㱋ᗂ࡛ࡣ 80㸣ࡢṚஸ⋡ࡀࠊ2 㱋ᗂ࡛ࡣ 60%ࡢṚஸ ⣙ 50cm㸧ࠊ๓㏙ࡢ᪉ἲ࡛ヨస〇 1,000 ಸᕼ㔘ᾮ ⋡ࡀᚓࡽࢀࡓ㸦ᅗ 7㸧 ࠋࡲࡓࠊ1 ᪥㛫ᦤ㣗ࡍࢀࡤ 2 㱋ࠊ3 㸦1 107 ከゅయ / ml㸧࠾ࡼࡧ 125 ಸᾮ㸦8 107 ከゅ 㱋ࡶ 90㸣௨ୖࡢṚஸ⋡ࡀᚓࡽࢀࡓࠋ య / ml㸧ࢆᩓᕸࡋࡓࠋᩓᕸ 3 㛫ᚋୖⴥ㸦ᰴඖࡽ ᒱ㜧┴㎰ᴗᢏ⾡ࢭࣥࢱ࣮◊✲ሗ࿌ ➨ 12 ྕ㸸27㹼33 㸦2012㸧 ẚ㍑ⓗࡁ࠸ዃ㞧≀ࢆ㝖ࡁࠊୖΎࢆ 3,500rpm 㐲ᚰศ㞳 ⾲㻟䚷ᅡሙ䛻䛚䛡䜛㻿㼜㼘㼠㻺㻼㼂䛾ᩓᕸᚋṧຠᛶ䛾ⴥู᥎⛣ ฎ⌮༊ ⴥ ᬑ㏻ᩓᕸ 㧗⃰ᗘᑡ㔞ᩓᕸ 䝝䝇䝰䞁䝶䝖䜴ᗂṚஸ⋡䠄䠂䠅 㻣᪥ᚋ 㻝㻜᪥ᚋ 㻞㻜᪥ᚋ ࢆ⧞ࡾ㏉ࡋ࡚ከゅయࢆྵࡴỿẊࢆᚓࡓࠋ⢭〇ᙜ᪥⢭〇 㻜᪥ᚋ 㻟᪥ᚋ 㻡᪥ᚋ ୖ 㻢㻣㻚㻡 㻞㻜㻚㻜 㻡㻚㻟 㻝㻡㻚㻜 㻡㻡㻚㻢 㻜 ⁐ᾮࢆ3㱋ࣁࢫࣔࣥࣚࢺ࢘ᗂࢻࣟࢵࣉࣞࢵࢺࣇ࣮ ୰ 㻤㻞㻚㻝 㻞㻡㻚㻜 㻟㻜㻚㻤 㻞㻜㻚㻜 㻞㻡㻚㻜 㻞㻢㻚㻟 ࢹࣥࢢἲࡼࡾ┤᥋㣧ୗࡉࡏࡓࠋฎ⌮ᗂࢆಶู㣫⫱ ୗ 㻡㻥㻚㻜 㻞㻡㻚㻜 㻟㻡㻚㻜 㻞㻥㻚㻠 㻟㻝㻚㻟 㻡㻚㻥 ୖ 㻠㻣㻚㻡 㻝㻣㻚㻡 㻝㻢㻚㻞 㻝㻜㻚㻡 㻞㻥㻚㻠 㻝㻜㻚㻜 ୰ 㻡㻜㻚㻜 㻡㻚㻜 㻝㻡㻚㻠 㻤㻚㻜 㻠㻜㻚㻜 㻜 ୗ 㻣㻣㻚㻡 㻝㻞㻚㻡 㻞㻞㻚㻡 㻟㻝㻚㻟 㻝㻝㻚㻤 㻠㻣㻚㻝 ࡋࠊ㢧ᚤ㙾ࢆ⏝࠸࡚Ṛஸෆࡢከゅయࡢ᭷↓ࢆ☜ㄆࡋࠊ NPV ࡼࡿṚஸ⋡ࢆㄪᰝࡋࡓࠋྠᵝࡢ᪉ἲࢆ⏝࠸࡚ࠊ ↓ฎ⌮༊ࡢᅵተᑐࡋ࡚᪤▱ࡢ⃰ᗘࡢ A ࢱࣉ C ࢱ ࣉࢆΰྜࡋࡓከゅయᾮࢆຍ࠼࡚ࢵࢭࢆ⾜࠸ࠊṚஸ ⋡ࡽከゅయ⃰ᗘࢆ⟬ฟࡍࡿࡓࡵࡢ᳨㔞⥺ࢆసᡂࡋࡓࠋ 100 ᅵተࡢ㊴ࡡ㏉ࡾࡢᙳ㡪ࢆ᫂ࡽࡍࡿࡓࡵࠊ2005 Ṛஸ⋡䠄䠂䠅 80 ᖺ 7 ᭶ 25 ᪥ࠊ⏕≀ᕤᏛ◊✲ᡤᅡሙ㸦ᒱ㜧┴⨾⃰ຍⱱ 60 ᕷ㸧࠾࠸࡚ࢲࢬ㸦ࣇࢡࣘࢱ࢝ࠊ6 ᭶ 9 ᪥✀ࠊⲡ 40 ⣙ 55cm㸧␏ヨస〇 1,000 ಸࡲࡓࡣ 250 ಸᕼ㔘ᾮࢆ 20 10a ᙜࡓࡾ 200L ࢆ┤᥋ᩓᕸࡋࡓࠋᩓᕸᚋ 2ࠊ7 ࠾ࡼࡧ 14 ᪥ᚋୖⴥ㸦ᰴඖࡽୖ 20cm㹼30cm ࡲ࡛㸧ୗ 0 ᩓᕸ๓ 0 1 2 4 6 ᅗ 6 ࢲࢬୖⴥ࠾ࡅࡿ SpltNPV ࡢṧຠᛶࡢኚ ᙳ㡪ࢆㄪࡿࡓࡵࠊྠࢲࢬᅡሙ࡚ 7 ᭶ 28 ᪥〇 1,000 ಸᕼ㔘ᾮ㸦ᒎ╔ࡋ࡚ࣉ࣮ࣟࢳ 1,000 ಸࢆ 100 Ṛஸ⋡䠄䠂䠅 ⴥ㸦ᰴඖࡽ 20cm ୖࡲ࡛㸧ࢆ᥇㞟ࡋࠊ๓㏙ࡢ᪉ἲ ࡼࡾ 3 㱋ᗂࢆ⏝࠸࡚Ṛஸ⋡ࢆㄪᰝࡋࡓࠋࡲࡓࠊ㝆㞵ࡢ ᩓᕸᚋ㛫(㛫) ຍ⏝㸧ࢆᩓᕸࡋ࡚⸆ᾮࡀ༑ศ࠸ࡓᚋࠊ୍㒊ࡢᰴỈ㐨 80 Ỉࢆᰴୖࡽ1ᰴᙜࡓࡾ2Lᧈ࠸ࡓࠋ ࢲࢬⴥࢆᅇࡋࠊ 60 3 㱋ᗂࢆ⏝࠸࡚Ṛஸ⋡ࢆㄪᰝࡋࡓࠋ [⤖ᯝ] 40 సᡂࡋࡓ᳨㔞⥺㸦ᅗ 8㸧ࡼࡾᅵተ୰ከゅయ⃰ᗘࢆ⟬ 20 ฟࡋࡓ⤖ᯝࠊᩓᕸ┤ᚋࡢᅵተࡣࠊᬑ㏻ᩓᕸ༊ࠊ㧗⃰ᗘ ᑡ㔞ᩓᕸ༊ࡑࢀࡒࢀ 2.14 105ࠊ4.90 105 ከゅయ / g 0 ↓ฎ⌮ 㻢㛫ᚋ 㻝᪥ᚋ 㻞᪥ᚋ ᩓᕸᚋᗂᅇᮇ 㻞㱋ᗂ 㻟㱋ᗂ ᅗ 7 ᅡሙ࠾ࡅࡿᦤ㣗㛫ࡢ㛗ࡉṚஸ⋡ࡢ㛵ಀ (4) 㝆㞵ࡢᙳ㡪ᅵተ୰ࡢ࢘ࣝࢫࡢṧຠᛶ ⾲ࠉᅵተ୰ࡢከゅయ⃰ᗘࡢ᥎⛣ ከゅయ⃰ᗘ䠄ಶ㻌㻛㻌⇱ᅵተ㼓䠅 ᩓᕸ᪉ἲ ᩓᕸ┤ᚋ 㻟᪥ᚋ 㻝㻜᪥ᚋ ᬑ㏻ᩓᕸ 㻞㻝㻟㻘㻢㻢㻣 㻝㻘㻣㻟㻝 㻥㻜㻢 㧗⃰ᗘᑡ㔞ᩓᕸ 㻠㻥㻜㻘㻟㻥㻢 㻙 㻥㻤㻡 㻞㻜᪥ᚋ 㻟㻠㻡 㻙 䚷䠉䠖 ᐃ್䛺䛧 [┠ⓗ] NPV ࡣ⣸እ⥺ࡼࡿᙳ㡪ࢆཷࡅࡃ࠸ᅵተ୰࡛ࡣẚ 100 ㍑ⓗάᛶࡀᏳᐃࡋ࡚࠸ࡿ 7)⪃࠼ࡽࢀࡿࡇࡽࠊ〇 ࡀࡾࡼࡿṧຠᛶࢆ᫂ࡽࡍࡿࠋ [ᮦᩱ࠾ࡼࡧ᪉ἲ] ๓㏙ࡢ 2004 ᖺᗘ㜵㝖ຠᯝ☜ㄆヨ㦂ࢆ⾜ࡗࡓ㎰ᴗࢭ ࣥࢱ࣮ෆࢲࢬᅡሙࡢ␇ᅵ㸦pH6.8㸧ࢆ౪ヨࡋࡓࠋᬑ㏻ ᩓᕸ༊ࠊ㧗⃰ᗘᑡ㔞ᩓᕸ༊࠾ࡼࡧ↓ฎ⌮༊ࡢྛ 2 ᮲ࡢ␏ ࡽ῝ࡉ⣙ 3cm ࡲ࡛ᅵተࢆ᥇ྲྀࡋࠊ40Υ࡚ 48 㛫 ⇱ࡋࡓࠋ⢊○ࡋࡓ⇱ᅵተࢆ 0.02% Tween80 ⁐ᾮᠱ ⃮ࡋࠊ4Υ࡚ 90 ศ㛫㟼⨨ࡋࡓᚋ 600rpm 㐲ᚰศ㞳࡚ 80 Ṛஸ⋡ (%) ᩓᕸᚋࡢ㝆㞵ࡼࡿᙳ㡪ྠ㉳ࡇࡿᅵተࡢ㊴ࡡୖ 60 40 y = 19.614x - 42.999 R² = 0.9815 20 0 2 3 4 5 6 7 8 ᅵተ୰ከゅయ⃰ᗘ (Log ಶ/⇱ᅵተg) ᅗ ᅵተ୰ከゅయ⃰ᗘṚஸ⋡ࡢ㛵ಀ ኳᩛ࢘ࣝࢫࢆ⏝࠸ࡓࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿᚤ⏕≀㎰⸆ࡢ㛤Ⓨ ᅵተࡢ⃰ᗘ࡛ከゅయࡀᏑᅾࡋ࡚࠸ࡓࠋ3 ᪥ᚋࡣ⣙ 200 ᮏࡣࠊࣁࢫࣔࣥࣚࢺ࢘ⱝ㱋ᗂᑐࡍࡿẅຠᯝࡣ ศࡢ 1 ῶᑡࡋࡓࡀࠊࡑࡢᚋࡢῶᑡࡣ⦆ࡸ࡛ࠊᩓᕸ 10 㧗࠸ࡀࠊ୰㱋ᮇ௨㝆࡛ẅຠᯝࡣపୗࡍࡿࡓࡵࠊᦤ㣗㔞 ᪥ᚋࡲ࡛⣙ 1 103 ከゅయ / g ᅵተࡢ⃰ᗘ࡛ከゅయࡀ᳨ ࡢⅬࡽࡶⱝ㱋ᮇᩓᕸ㜵㝖ࡍࡿᚲせࡀ࠶ࡿࠋࡲࡓࠊᗂ ฟࡉࢀࡓ 㸦⾲ 4㸧 ࠋ ヨ㦂ᮇ㛫୰ࡣࠊ ᩓᕸ 2 ᪥ᚋ 32.0mmࠊ ࡢⓎ⫱㐺 ࡛ẅຠᯝࡀ㧗ࡃ࡞ࡿࡇࡽࠊࣁࢫࣔࣥ 3 ᪥ᚋ 58.0mm ࡢ㝆Ỉ㔞ࡀ࠶ࡗࡓࠋ ࣚࢺ࢘ᑐࡋ࡚ࡣᖹᆒ 25Υ௨ୖࡢẼ ࡢ㧗࠸ᮇ ࡘࡂࠊ⸆ࢆᅵተ┤᥋ᩓᕸࡋ࡚ࠊ㝆㞵ࡼࡿᅵተ ⏝ࡍࡿຠᯝࡀ㧗ࡃ࡞ࡿࠋᮇࡢ ᐊෆ࡛⏝ࡍࡿሙྜ ୰ከゅయࡢ㊴ࡡ㏉ࡾຠᯝࢆㄪᰝࡋࡓࠋᩓᕸ 1 ᪥ᚋ ࡞ࠊ タᐃ ᗘ 17Υࡢຍ ᮲௳ୗ࡛༑ศ࡞ຠᯝࢆᚓࡿࡓ 14mmࠊ6 ᪥ᚋ 41mm, 11 ᪥ᚋ 9mm ࡢ㝆Ỉ㔞ࡀ࠶ ࡵࡣࠊ2 㱋ᗂᮇࡲ࡛⸆ᩓᕸࢆ⾜࠺ᚲせࡀ࠶ࡿࠋ ࡾࠊᅵተࡢ㊴ࡡୖࡀࡾࡀㄆࡵࡽࢀࡓࠋࢲࢬୗⴥ࡛ᩓᕸ NPV ࡢຠᯝࡢᣢ⥆ᛶࡣᵝࠎ࡞⎔ቃせᅉࡀᙳ㡪ࡍࡿࠋ ᚋ 2 㐌㛫௨ୖࢃࡎ࡞ẅຠᯝࡀᚓࡽࢀࡓࡀࠊ10㸣๓ᚋ ከゅయࡣ⣸እ⥺ᙅࡃࠊ⣸እ⥺᭚ࡉࢀࡿኻάࡋࠊẅ ࡢṚஸ⋡ࡲࡾ㜵㝖ຠᯝࡣᮇᚅ࡛ࡁ࡞ࡗࡓ㸦⾲ 5㸧 ࠋ ຊࢆኻ࠺ࠋࡑࡢࡓࡵᅡሙᩓᕸࡉࢀࡓከゅయࡣࠊᛴ㏿ ࡲࡓࠊேᕤⓗ࡞㝆㞵ࡼࡿẅຠᯝࡢᙳ㡪ࢆㄪᰝࡋࡓ ẅຊࡀపୗࡍࡿࠋᮏࡘ࠸࡚ࡶࠊ㸲㛫ࡢኴ㝧ග ࡇࢁࠊࢲࢬⴥᩓᕸࡋࡓ〇ࡣ୍ᗘ⇱ࡍࢀࡤࠊࡑ ⥺↷ᑕ࡛ୖⴥ࡛ࡣẅຠᯝࡀ༙ῶࡋࡓࡀࠊᩓᕸᚋࡢ␗ ࡢᚋࡢ㝆㞵ࡼࡿຠᯝࡢపୗࡣࠊ⣙ 20%ࡲࡗࡓ ࡞ࡿᮇᅡሙࡼࡾᅇࡋࡓᗂࡢṚஸ⋡ࡽࠊᮏࡣ 㸦ᅗ 9㸧 ࠋ ᩓᕸ┤ᚋࡽ6㛫ࡢᦤ㣗࡛༑ศ࡞Ṛஸ⋡ࡀᚓࡽࢀࡿከ ゅయ⃰ᗘ࡛࠶ࡿࡇࡀศࡗࡓࠋࡑࡢࡓࡵࠊ⸆ᩓᕸࢆ ⥲ྜ⪃ᐹ ኤ᪉⾜࠺ࡇࠊ⸆ࢆⴥ㠃ᩓᕸࡍࡿࡇ࡛༑ศ࡞ ᚤ⏕≀㎰⸆ࡢ≉㛗ࡋ࡚⎔ቃࡸேయࡸࡉࡋ࠸ࠊᢠ ຠᯝࡀᚓࡽࢀࡿࠋ ᛶᐖฟ⌧ࡢྍ⬟ᛶࡀప࠸࠸ࡗࡓ㛗ᡤࡀ࠶ࡿࠋࡑࡢࡓ ࢲࢬୗⴥ࡛ࡣᩓᕸࡋࡓከゅయࡼࡿᙅ࠸ṧຠᛶ ࡵࠊῶ㎰⸆᱂ᇵ࠾ࡅࡿ IPM ࡢ㜵㝖య⣔⤌ࡳධࢀ࡚ ࡀ 20 ᪥㛫ᣢ⥆ࡋࡓࡀࠊᅵተࡢ㊴ࡡୖࡀࡾࡼࡿຠᯝࡶ ⏝࡛ࡁࡿ㈨ᮦࡋ࡚ᮇᚅࡉࢀ࡚࠸ࡿࠋࣁࢫࣔࣥࣚࢺ࢘ ᙅࡃࠊ㜵㝖ຠᯝࢆᚓࡿࡣ༑ศ࡛࡞ࡗࡓࠋࡑࡢࡓࡵࠊ ࡣࠊ㫣⩟┠᪻ຠᯝࡢ㧗࠸ BT ࡼࡿ㜵㝖ຠᯝࡀ༑ ⸆ࢆᩓᕸࡋࡓᅡሙ࠾࠸࡚ࠊᩓᕸᚋࡢ␗࡞ࡿᮇᏤ ศ࡛࡞࠸ࡓࡵࠊᚤ⏕≀㎰⸆ࡋ࡚ࡣࠊNPV ࢆࡣࡌࡵ ࡋࡓᗂࡢṚ⋡ࢆㄪࠊ㜵㝖ຠᯝࡢᣢ⥆ᮇ㛫ࢆ᳨ウ ࡋࡓኳᩛ࢘ࣝࢫࡢ⏝ࡀ᭱ࡶᮇᚅ࡛ࡁࡿࠋᅇ㛤Ⓨࡋ ࡋࡓ⤖ᯝࠊᩓᕸᚋ 2 ᪥┠ࡲ࡛ࡣ㧗࠸㜵㝖ຠᯝࡀᚓࡽࢀࡿ ࡓࠕࣁࢫ࣮ࣔࣥ࢟ࣛࠖࡢᡂศ࡛࠶ࡿ SpltNPV ࡣ⮬↛ ࡀࠊ3 ᪥┠௨㝆ຠᯝࡣపୗࡋࠊᏤᗂᑐࡍࡿຠᯝࡢ ⏺ࡼࡾศ㞳ࡋ࡚㑅ᢤࡋࡓᰴ࡛࠶ࡾࠊ⮴Ṛ㛫ࡀ᪩࠸ࡓࡵࠊ ᣢ⥆ᮇ㛫ࡣ 1 㐌㛫⛬ᗘࡔࡗࡓ㸦ࢹ࣮ࢱᮍᥖ㍕㸧 ࠋ ࡇࢀࡲ࡛ၥ㢟࡞ࡗ࡚࠸ࡓ㐜ຠᛶࡀᨵၿࡉࢀࠊࡲࡓẅ ຊࡶ㧗ࡃ࡞ࡗ࡚࠸ࡿࠋ NPV ࡣస≀ୖ࡛Ṛஸࡋࡓᗂࡽከゅయࡀᨺฟࡉࢀࠊ ḟឤᰁࡢຠᯝࡼࡾᩓᕸࡋࡓࡁྠࡌຠᯝࡀᮇᚅ ࡛ࡁࡿࠋࡋࡋࠊ2ࠥ3 㱋ࡢᮇ⸆ࢆᩓᕸࡋࠊࡑࡢ 16 ᪥ᚋᨺࡋࡓࡇࢁࠊᗂࡢṚ⋡ࡣ 30㸣⛬ᗘ࡛ ⾲㻡䚷ᅵተᩓᕸ㻿㼜㼘㼠㻺㻼㼂䛻䜘䜛䝎䜲䝈ⴥ䜈䛾㊴䛽㏉䜚ຠᯝ ᕼ㔘ಸ⋡ 㻝㻜㻜㻜ಸ 㻞㻡㻜ಸ ⴥ ୖ ୗ ୖ ୗ 䝝䝇䝰䞁䝶䝖䜴ᗂṚஸ⋡䠄䠂䠅 㻜᪥ᚋ 㻞᪥ᚋ 㻣᪥ᚋ 㻝㻠᪥ᚋ 㻜 㻝㻝㻚㻡 㻣㻚㻠 㻣㻚㻝 㻟㻚㻟 㻜 㻟㻚㻟 㻝㻜㻚㻣 㻣㻚㻠 㻝㻞㻚㻜 㻝㻜㻚㻟 㻟㻚㻢 㻝㻞㻚㻡 㻢㻚㻣 㻣㻚㻠 㻝㻜㻚㻜 ࠶ࡗࡓ㸦ࢹ࣮ࢱᮍᥖ㍕㸧 ࠋṚஸᗂ 1 ಶయᙜࡓࡾࡢከゅ యᨺฟ㔞ࡣឤᰁࡢⓎ⫱㱋ࡼࡗ࡚␗࡞ࡾࠊ3 㱋ᗂ ẚ 4 㱋ᗂ࡛ 3.4 ಸࠊ5 㱋ᗂ࡛ 7.5 ಸ࡛࠶ࡗࡓࡇ ࡽࠊ୰㱋ᮇ௨㝆ឤᰁࡍࢀࡤḟឤᰁຠᯝࡣ㧗ࡲࡿྍ ⬟ᛶࡀ࠶ࡿࡀࠊ㣗ᐖ㔞ࡸឤཷᛶࢆ⪃៖ࡋࡓ㜵㝖㐺ᮇ࡛࠶ ࡿ 2ࠥ3 㱋ᗂ⸆ᩓᕸࢆࡋࡓሙྜࡣࠊṚஸᗂ ࡼࡿḟឤᰁࡢຠᯝࡣపࡃࠊṧຠஈࡋ࠸ࠋࡇࡢࡓࡵࠊ 100 ᗂࡢⓎ⏕ࡀ⥅⥆ࡋ࡚ㄆࡵࡽࢀࡿሙྜࡣࠊᩓᕸࢆ⧞ࡾ Ṛஸ⋡䠄䠂䠅 80 ㏉ࡍᚲせࡀ࠶ࡿ⪃࠼ࡽࢀࡿࠋ 60 ᮏࡣࠊᥬᩫᕝᕤᴗ㸦ᰴ㸧ࡼࡾၟရࡀ㐍ࡵࡽࢀ࡚ 40 ࡁࡓࡀࠊᅡሙ࠾ࡅࡿ㜵㝖ຠᯝࢆホ౯ࡍࡿࡓࡵࠊᙜᴗ 20 ࡼࡿ᪥ᮏ᳜≀㜵༠ࡢጤクヨ㦂ࡋ࡚ࠊ2004 ᖺ ࡽ 2008 ᖺࡲ࡛ᅜࡢබⓗ㎰ᴗヨ㦂◊✲ᶵ㛵࠾࠸࡚ࠊ 0 ↓ฎ⌮ Ỉᩓᕸ ᅗ 㝆㞵ࡼࡿᙳ㡪 ࢲࢬࠊࢳࢦࠊࣞࢱࢫࠊࢩࢯ࠾ࡼࡧࣂࢪࣝࡢ 5 స┠ࠊ ィ 21 ࡢ⸆ຠ⸆ᐖᅡሙヨ㦂ࡀᐇࡉࢀࡓ㸦⾲ 6㸧 ࠋヨ㦂 ᒱ㜧┴㎰ᴗᢏ⾡ࢭࣥࢱ࣮◊✲ሗ࿌ ➨ 12 ྕ㸸27㹼33 㸦2012㸧 ⤖ᯝࡼࡾࠊࢲࢬࢆࡣࡌࡵࡢస≀࡛ࡶ A ุᐃࡀᩘከࡃ FXWZRUP 6SRGRSWHUD OLWXUD /HSLGRSWHUD 1RFWXLGDHLQ-DSDQ%LRO&RQWURO㸸 ᚓࡽࢀ࡚࠾ࡾࠊ㜵㝖ຠᯝࡢ㧗ࡉࡀㄆࡵࡽࢀ࡚࠸ࡿࠋヨ㦂 ሙᡤࡣ㟢ᆅࡲࡓࡣタ࠾࠸࡚ᐇࡉࢀࡓࡀࠊࡶ㧗 *HWWLJ55DQG:-0F&DUWK\*HQRW\SLF ࠸㜵㝖ຠᯝࡀᚓࡽࢀࠊస┠ࠊẼ ࠊኳᩛࡢ᭷↓࡞ࡢ᮲ YDULDWLRQDPRQJZLOGLVRODWHVRI+HOLRWKLVVSS ௳ࡀ␗࡞ࡗ࡚࠸࡚ࡶ㜵㝖ຠᯝࡣᏳᐃࡋ࡚࠸ࡓࠋẼ ࡀప QXFOHDU SRO\KHGURVLV YLUXVHV IURP GLIIHUHQW ୗࡍࡿ⛅௨㝆ࡢᩓᕸ࡛ࡣ⸆ຠࡢపୗࡀᠱᛕࡉࢀࡓࡀࠊኟ JHRJUDSKLFDOUHJLRQV9LURORJ\㸸 Ꮨ㸦7㹼9 ᭶㸧ࡢヨ㦂ࡀከࡗࡓࢲࢬẚ㍑ࡋ࡚ࠊ⛅Ꮨ &KHUU\&/DQG0'6XPPHUV*HQRW\SLF 㸦9㹼10 ᭶㸧ࡢヨ㦂ࡀከࡗࡓࣞࢱࢫࠊࢳࢦ࡛ࡶ༑ศ YDULDWLRQ DPRQJ ZLOG LVRODWHV RI WZR QXFOHDU ࡞㜵㝖ຠᯝࡀ☜ㄆ࡛ࡁࡓࠋ୍᪉ࠊᑐ↷ࡋ࡚⏝ࡋࡓ SRO\KHGURVLV YLUXVHV LVRODWHG IURP 6SRGRSWHUD OLWWRUDOLV-,QYHUWHEU3DWKRO㸸 BT 㛵ࡋ࡚ࡣࠊࣁࢫࣔࣥࣚࢺ࢘ᑐࡍࡿẅຠᯝࡀ 㧗࠸ࡉࢀࡿ⸆㸦ࢮࣥࢱ࣮ࣜ㢛⢏Ỉࠊࣇ࣮ࣟࣂࢵ 0DHGD 6 <0XNRKDUD DQG $.RQGR ࢡ DF ࠾ࡼࡧࣞࣆࢱ࣮࣒ࣇࣟࣈࣝ㸧ࢆ⏝࠸ࡓࡀࠊ⸆ຠ &KDUDFWHULVWLFDOO\ GLVWLQFW LVRODWHV RI WKH ࡢⓎ⌧ࡣ᪩࠸ࡶࡢࡢࠊ㜵㝖ຠᯝࡣᮏࡀඃࢀ࡚࠸ࡿࡇ QXFOHDUSRO\KHGURVLVYLUXVIURP6SRGRSWHUDOLWXUD ࡀ☜ㄆࡉࢀࡓࠋ -*HQ9LURO㸸 ࡲࡓࠊࢲࢬᅡሙ࠾࠸࡚ࠊᮏࡢᗂ㱋ูࡢ㜵㝖ຠ &RU\ 6- 56+DLOV DQG 06DLW ᯝࡘ࠸࡚ヨ㦂ࡋࡓ⤖ᯝࠊᐇ㝿ࡢ⏝࡛ᐃࡋ࡚࠸ࡿᇶ %DFXORYLUXVHFRORJ\,Q7KH%DFXORYLUXVHV0LOOHU ᮏ⃰ᗘ㸦〇ࡢ 1,000 ಸᕼ㔘㸧࡛⏝ࡍࡿሙྜࡣ 3 㱋ᗂ ./HG3OHQXP1HZ<RUN ࡲ࡛༑ศ࡞㜵㝖ຠᯝࡀㄆࡵࡽࢀࡓࠋࡲࡓࠊ2 㱋ᗂࡢ 㜵㝖ࡣ༙ศ⃰ᗘ㸦2,000 ಸᕼ㔘㸧࡛⏝ࡋ࡚ࡶ༑ศ࡞ Abstract ຠᯝࡀㄆࡵࡽࢀ࡚࠾ࡾ㸦ࢹ࣮ࢱᮍᥖ㍕㸧 ࠊᗂࡢ᪩ᮇⓎ We developed a microbial pesticide, ‘Hasumon Killer ぢࡼࡿ㜵㝖ࡀࢥࢫࢺࡢ㍍ῶࡶ⧅ࡀࡿ⪃࠼ࡽࢀࡿࠋ (Ibigawa Kogyo Co., Ltd)’ for common cutworm, ⾲㻢䚷⸆ຠ⸆ᐖᅡሙヨ㦂⤖ᯝ Spodoptera litura. Nucleopolyhedrovius (NPV) clones స┠ྡ were isolated from S. litura larvae collected in Japan. ุᐃ䠄ᩘ䠅 ヨ㦂ᆅ 㻭 㻮 㻯 Ⲉᇛ䚸ᒱ㜧䚸㈡䚸ᚨᓥ 㻡 㻝 㻝 䜲䝏䝂 Ⲉᇛ䚸ᒱ㜧䚸ᚨᓥ䚸ᒣཱྀ䚸⚟ᒸ 㻝 㻠 㻞 clones were classified into three types designated as 䝺䝍䝇 රᗜ䚸㤶ᕝ䚸ᗈᓥ 㻞 㻝 㻜 type A, type B and type C SpltNPV. REN analysis Ⲉᇛ䚸ឡ▱ 㻞 㻜 㻜 㻝 㻝 㻜 䝎䜲䝈 䝅䝋 䝞䝆䝹 ឡ▱ ᕼ㔘ಸ⋡䠖㻝㻘㻜㻜㻜ಸ Restriction endonuclease (REN) patterns of these showed that type C SpltNPVs (SpltNPV-C) were distinct from NPVs previously identified from S. litura in Asia. SpltNPV-C caused rapid mortality of S. ㅰ㎡ litura and SpltNPV-A showed highest virulence for S. ᮏ◊✲ࢆᐇࡍࡿ࠶ࡓࡾࠊඖᥬᩫᕝᕤᴗᰴᘧ♫ࡢ litura larvae. We obtained a patent for idea on that ♽∗ỤຬẼẶࡣࠊ࢘ࣝࢫࡢ〇ࠊ㔞⏕⏘࡞ከ blending of SpltNPV-A clone with SpltNPV-C clone ࡞ࡿ༠ຊࢆ㡬࠸ࡓࠋࡇࡇ῝ࡃឤㅰ⏦ࡋୖࡆࡿࠋ improved the potential of NPVs for biological control of S. litura as compared with preparation containing ᘬ⏝ᩥ⊩ single clone from SpltNPV-A. ‘Hasumon Killer’ ὸᒣ ဴ㸪ኳ㔝㝯㸪ᮏ㞞❶㸪㟷ᮌᘯ㸪℈⏣༓⿱㸪 containing these two clones is scheduled to be ᒸ⏣㰺ኵ᰾ከゅయ࢘ࣝࢫࡼࡿࣁࢫࣔ registered as a biopesticide for S. litura by April, 2012. ࣥࣚࢺ࢘ࡢ㜵㝖 ឡ▱㎰⥲ヨ◊ሗ 㸸 Field testing showed that this product was effective as ᒸ⏣㰺ኵ᰾ከゅయ࢘ࣝࢫࡼࡿࣁࢫࣔ a microbial pesticide for S. litura. ࣥࣚࢺ࢘ࡢ㜵㝖㛵ࡍࡿ◊✲ ୰ᅜ㎰ヨሗ (㸸 .DPL\D.-=KX00XUDWD%$/DYLQD&DRLOL 0,NHGD0.RED\DVKLDQG6.DZDPXUD&ORQLQJ DQGFRPSDUDWLYHFKDUDFWHUL]DWLRQRIWKUHHGLVWLQFW QXFOHRSRO\KHGURYLUXVHVLVRODWHGIURPWKHFRPPRQ Key words Spodoptera litura, NPV, Hasumon Killer, microbial pesticide
© Copyright 2024 ExpyDoc
