MGC PhD Course Technology Facilities February 5, 2014, Erasmus MC, Rotterdam GK gebouw (gebouw Rochussenstraat) CQ-1Da February 5: Genomics and Transcriptomics Organizers: Dr. W. van IJcken, ErasmusMC, Rotterdam Dr. H.P.J. Buermans, LUMC, Leiden 09:00 - 09:30 09:30 - 09:50 09:50 - 10:10 10:10 - 10:40 Arrival & Registration Frank Sleutels: Biomics Sophie Greve: LGTC Kristiaan van der Gaag: Introduction NGS Illumina/LifeTech 10:40 - 11:00 coffee/tea break 11:00 - 11:30 Yahya Anvar: Single molecule sequencing 11:30 - 12:00 Henk Buermans: Fluidigm C1 Single cell genomics/transciptomics 12:00 - 12:50 lunch 12:50 - 13:20 Ralph Stadhouders: ChIP-seq 13:20 - 13:50 Marjon van Slegtenhorst: NGS in diagnostics 13:50 - 14:20 Peter-Bram ‘t Hoen: Expression profiling 14:20 - 14:40 coffee/tea break 14:40 - 15:10 Rutger Brouwer: Exome Sequencing 15:10 - 15:40 Mirjam van den Hout: NGS data analysis 15:40 - 16:10 Wilfred van IJcken: Future developments NGS MGC PhD Course Technology Facilities February 6, 2014, Erasmus MC, Rotterdam GK gebouw (gebouw Rochussenstraat) CQ-1Da February 6: Proteomics Organizers: Dr. J. Demmers, ErasmusMC, Rotterdam Dr. P. Hensbergen, LUMC, Leiden TBC 9.30-10.30 10.30-11.15 (t.b.c.) Introduction to mass spectrometry techniques Dr. P. Hensbergen, LUMC, Introduction Proteomics 11.15-11.30 Break 11.30-12.15 Dr. P Hensbergen, LUMC, Mass spec analysis of post-translational modifications 12.15-13.00 Lunch 13.00-13.45 13.45-14.30 LAB TOUR Dr. J. Demmers, Erasmus MC, Quantitative proteomics 14.30-15.00 coffee/tea 15.00-15.45 Dr. K. Bezstarosti, Erasmus MC, Protein and peptide separation techniques and sample preparation 15.45-16.15 proteomics Dr. J. Demmers, Erasmus MC, Data analysis and interpretation in 16.15-16.45 General discussion MGC PhD Course Technology Facilities February 7, 2014, Erasmus MC, Rotterdam GK gebouw (gebouw Rochussenstraat) CQ-1Da February 7: Imaging Organizers: Dr. G. van Cappellen and Dr. A.B. Houtsmuller, Erasmus MC, Rotterdam Prof. Dr. C. Lowik, LUMC, Leiden Live cell imaging 9:00-9:35 Gert-Jan Kremers (Erasmus MC): Introduction to live cell fluorescence imaging 9:35-10:10 Adriaan Houtsmuller (Erasmus MC): Quantitative fluorescence methods and high resolution 10:10-10:45 Sylvia Le Devedec (LACDR, LUMC): Systems Microscopy of cell migration 10:45-11:00 coffee/tea 11:00-11:35 Roman Koning (LUMC): Correlative Cryo Light Electron microscopy 11:35-12:10 Timo ten Hagen (Erasmus MC): Multiphoton intravital microscoscopy 12.10-13.00 Lunch In vivo imaging – whole body optical imaging and MRI technology (tbc) 13.00-13.15 Clemens Lowik: Introduction Molecular Imaging in vivo incl. PET/SPECT, CT, MRI, Ultrasound en optical imaging 13.15-14.00 Clemens Lowik: Whole body Bioluminescentie imaging and its applications 14.00-14.45 Eric Kaijzel: Whole body Fluorescence imaging and its applications 14.45-15.00 coffee/tea 15.00-15.45 Fijs van Leeuwen: Clinical translation of novel imaging strategies; from molecule to man Louise van de Weerd: MRI en ultra high field MRI Boudewijn Lelieveldt: Image fusion of heterogeneous multi-modal molecular imaging data 15.45-16.30 16.30-17.15