Isothermal Master Mix For research purposes only. Not for use in diagnostic procedures for clinical purposes. FOR IN VITRO USE ONLY Product Description: Monitor reaction on Genie® II or a thermally The Isothermal Master Mix is an optimized Master controlled fluorimeter with 488nM excitation and Mix designed to simplify the preparation of an 520nM emission. Alternatively use a real-time PCR isothermal amplification assay. The master mix machine set to the FAM channel. contains a proprietary fast, novel DNA polymerase, proprietary thermostable inorganic pyrophosphatase, optimised reaction buffer, MgCl2, dNTPs and a Note: ds-DNA binding dye. Due to the presence of the thermostable inorganic pyrophosphatase there will be no accumulation of PPi. Package Contents: (100 rxns): Quality Control: - Isothermal Master Mix 1500 μl (Store at -20ºC) The Isothermal Master Mix has been tested for performance in Loop-mediated isothermal DNA - Tube Strip 20 strips (Store at room temperature) amplification. Monitored on a thermally controlled fluorimeter with Usage: 488nM excitation and 520nM emission. A typical 25 μl reaction would contain the following: Component Isothermal Master Mix Primers Template Total Volume 15.0 μl 5.0 μl 5.0 μl 25.0 μl Final conc. 1x * OptiGene Limited Unit 5 Blatchford Road Horsham West Sussex RH13 5QR Tel: +44(0)1403 274980 Fax: +44(0)1403 271017 * When using AMV Reverse Transcriptase in RTLAMP, add 0.25 unit per 25.0 μl. It’s usually possible to detect RNA by use only of Isothermal Master Mix, Notice to purchaser: limited license The purchase price of this product includes a limited, non-transferable license but the rate of the detection improves by adding a under reverse transcriptase. EP1,416,055, EP1,724,361 or their foreign counterparts, owned by Eiken European Patents EP1,020,534, EP1,873,260, EP2,045,337, Chemical Co., Ltd., to use only this amount of the product to practice LAMP technology described in said patents solely for the research and development Typical amplification conditions for this kit are: activities of the purchaser. No right to perform or offer commercial services of any kind using LAMP technology, including without limitation reporting the Amplification 30 minutes 65ºC Annealing 0.05ºC per section 98ºC - 80ºC results of purchaser's activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. Further information on purchasing licenses to practice the LAMP process may be obtained by contacting the Director of Licensing at Eiken Chemical Co., Ltd., 4-19-9 Taito, Taito-ku, Tokyo, 110-8408, Japan Isothermal Master Mix 本品は試験研究用試薬ですので、医薬品、その他の目的にはご使用になれません。日本語説明書製品説明: 本品は、等温遺伝子増幅を行うためのマスターミックスです。本品には、耐熱性鎖置換型 DNA ポリメラーゼ、耐熱性ピロホスファターゼ、MgCl2、dNTPs、至適化されたバッファー、二本鎖 DNA 結合性蛍光色素が含まれています。また、OptiGene 社が開発・製造した等温増幅蛍光測定装置 Genie® II 専用の Tube Strip が添付されています。製品内容：（100 回用）・ Isothermal Master Mix ・ Tube Strip（8 連チューブ） 1500 µl 20 本（-20℃保存）（室温保存）反応のモニタリングには、Genie® II あるいは 488 nm の励起波長において 520 nm の蛍光を検出する機器が使用できます。リアルタイム PCR 装置を使用する場合には、 FAM channel をご使用下さい。注意事項：本品は耐熱性ピロホスファターゼを含むため、ピロリン酸の生成による不溶物質の形成は起こりません。品質管理：本品は、488 nm の励起波長において 520 nm の蛍光を検出する機器を使用して、LAMP 法での遺伝子増幅能を確認しています。使用方法：標準反応スケールは 25 µl で、一反応あたり Isothermal Master Mix を 15 µl 使用します。 Isothermal Master Mix Primers 鋳型核酸 15.0 μl 5.0 μl 5.0 μl Total 25.0 μl OptiGene Limited Unit 5 Blatchford Road Horsham * * RT-LAMP 法において AMV Reverse Transcriptase を West Sussex RH13 5QR Tel: +44(0)1403 274980 Fax: +44(0)1403 271017 使用する場合は、25 µl の反応系あたり 0.25 unit を添加して下さい。Isothermal Master Mix に含まれる鎖置換型 DNA ポリメラーゼは逆転写活性を有するため、逆転写 Notice to purchaser: limited license The purchase price of this product includes a limited, non-transferable license 酵素を添加しなくても RNA を検出することができますが、 under 逆転写酵素を添加することで検出の速度が向上しま EP1,416,055, EP1,724,361 or their foreign counterparts, owned by Eiken す。 European Patents EP1,020,534, EP1,873,260, EP2,045,337, Chemical Co., Ltd., to use only this amount of the product to practice LAMP technology described in said patents solely for the research and development activities of the purchaser. No right to perform or offer commercial services of any kind using LAMP technology, including without limitation reporting the 本品の標準反応条件：増幅 30 分間 65℃ 会合曲線解析 98℃ - 80℃ 0.05℃/秒 results of purchaser's activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. Further information on purchasing licenses to practice the LAMP process may be obtained by contacting the Director of Licensing at Eiken Chemical Co., Ltd., 4-19-9 Taito, Taito-ku, Tokyo, 110-8408, Japan.