MB 070309 WG Dag - NBV

NBC-15
Biotechnology by Dutch Design
Tuesday/Wednesday, May27/28, 2014
Congress Centre “De Reehorst”, Ede
Downstream Processing Session on May 27, 2014
Organized by the Working Party on Downstream Processing of the
Dutch Biotechnological Society (NBV)
Session: Product Isolation / Design for Products and Processes
Program
14:35
Intro, Company Pitch
14:40
A process systems engineering approach to process and product
design
(Edwin Zondervan, TU Eindhoven)
15:05
High Throughput Biopharmaceutical Process Development
(Marcel Ottens, TU Delft)
15:30
Biorefinery of Microalgae: Mild separation technologies for
complex biomolecules
(Michel Eppink, Wageningen UR)
15:55
End of session
This session is chaired by Maarten Pennings (Tarpon Biosystems)
A process systems engineering approach to process
and product design
Dr. ir. Edwin Zondervan, Technical University Eindhoven
Process Systems Engineering is concerned with the systematic analysis and optimization of decision making
processes for the discovery, design, manufacture and distribution of (chemical) products.
In this talk I mainly want to demonstrate how the tools developed by the PSE community can help in the
systematic development of process flow sheets and the generation of new
product ideas. PSE has been
traditionally connected with typical process engineering areas such as the (petro) chemical industry, but
nowadays other branches of industry including the food- and pharmaceutical industry start valuing PSE.
After a short introduction to PSE, I will give a few successful examples of PSE for process and product design.
Two cases that I will discuss are 1) the use of process synthesis tools, such as mathematical programming and
super structure optimization for the generation of biorefinery production routes for biofuels from lignocellulosic
biomass; 2) the use of the product driven process synthesis method (PDPS) for the generation of process and
product ideas for nutraceuticals – polyphenols from tea and proteins from soy.
Overall, PSE is an exciting discipline that really aids decision making for process engineers.
High Throughput Process Development
for Biopharmaceuticals
Dr. Ir. Marcel Ottens, Technical University Delft
Minimizing cost of goods and improving speed to market are important boundary conditions for
biopharmaceutical process design. In the last decade platform processes were developed for monoclonal
antibodies that allowed easy adoption of the process for a new antibody.
With the advent of biosimilars, antibody fragment products, antibody drug conjugates (ADC’s) and other nonantibody biologicals, process development approaches are sought for that allow process design outside a
platform process (1). In order to achieve this, biopharmaceutical industry resorts to high throughput screening
(HTS), or High Throughput Process Development (HTPD). Adding mechanistic modeling to HTPD makes this
approach more versatile, in line with FDA regulatory guidelines, achieves a better process understanding, and
perfectly fits to the Quality by Design (QbD) approach.
This presentation will show the state-of-the-art of this High Throughput Biopharmaceutical Process Development
approach.
Biorefinery of Microalgae:
Mild separation technologies for complex biomolecules
Prof. Dr. Ir. Michel Eppink, Wageningen University and Research
“Biorefinery” is a facility that integrates cell disruption, extraction, conversion and separation technologies of
biomass. The biorefinery strategy is analogous to today's petroleum refinery, in which multiple fuel products and
chemicals are produced from crude petroleum. Biorefinery includes the selective isolation of products (proteins,
carbohydrates, lipids) from crude biomass. Biorefinery needs to be mild and efficient at the same time in order to
maintain the functionality of the products (e.g. native protein conformation) and thus value. For the development
of a sustainable biobased economy and to guarantee economical feasibility it is essential to use all biomass
ingredients for high value and bulk product applications. Biorefinery will result in ingredients for a variety of
applications (e.g. food, feed, fuel, pharma, chemicals) to cope with the worldwide scarcity of food and fuel in the
coming decades.
The feasibility of microalgae biorefinery production is presented from biomass concentration, cell wall
characterization, cell disruption, extraction towards fractionation technologies. Although algae are not yet
produced at large scale for high valuable or bulk applications there are opportunities to develop this process in a
sustainable way. If biorefinery of microalgae is applied, lipids should be fractionated into lipids for biodiesel, as
building blocks for the chemical industry and poly unsaturated fatty acids for the food and health market.
Whereas the fractionated functional proteins and carbohydrates can be applied for food, feed, health and bulk
chemicals. If in addition production of algae is done on residual nutrient feedstocks and CO2, production of
microalgae at large scale against low production costs the biorefinery approach for micro algae remains feasible.
In this presentation case studies will be presented showing the progress on the different biorefinery stages for
microalgae.
Information
The field of biotechnology has changed dramatically over the last two decades as a result of our understanding
of biological systems and processes and the insights that omics technologies have brought to us. We are more
and more capable of rationally designing biotechnological products and processes. Scientists working at Dutch
universities and research institutes are performing cutting edge science on biological systems and processes.
Researchers working in Dutch biotech companies and start-ups use this know-how to develop all kinds of
innovative products and processes.
An open and ongoing dialogue between researchers and between researchers and society is essential to
stimulate the exchange of ideas and to improve the sustainable production systems. The NBV organizes thus a
two-day conference to foster this dialogue. The congress will be held on May 27th and 28th 2014 at congress
centre 'De Reehorst' in Ede. For more information and the full program, please visit the NBV website
(www.nbvsite.nl).
Venue and Registration
The conference will be held in Congress Centre “De Reehorst” (Bennekomseweg 24, 6717 LM, Ede), a route
description can be found on www.reehorst.nl. Registration costs for a full pass (2 days) are € 495. Various
discounts are possible as 15% for NBV members and 50% for PhD students (non-NBV members) and 70% for
PhD students (NBV-members).
Separate a hotel reservation can be made at for example “De Reehorst”.
More information and registration online via http://nbv.kncv.nl
Working Party on Downstream Processing
The Working Party on Downstream Processing is part of the Dutch Biotechnological Society (NBV). Its objectives are:
•
To keep the members of the working party informed on international developments in the area of recovery
and purification of biological products;
•
To enable members to exchange ideas and discuss developments with other scientists in this area.
This is done a.o. by maintaining a web-site (www.nbv-pz.nl) and organizing events such as symposia, courses and lectures.
For additional information on the working party, please visit the web-site or contact any of the members:
Jeroen den Hollander ( [email protected])
Marcel Ottens ( [email protected])
Reinoud Noordman ( [email protected])
Marc Bisschops( [email protected])
Emile van de Sandt ( [email protected])
Michel Eppink ( [email protected])
Maarten Pennings ( [email protected])