LABEL-FRIENDLY ANTIMICROBIAL Lysoch™ G4 FERMENTED LYSOZYME 04 Key Benefits ・Allergen-free ・Growth control of Clostridium ・Prevent ‘late-blowing’ and off-flavors of cheese ・Prevent Lactobacilli spoilage during wine fermentation ・Meet consumers' demand for natural products 0402 The New Generation of Fermented Lysozyme For Low-temperature Processed Foods Currently, egg-white Lysozyme is allowed for the manufacture of cheese and wine as an anti-microbial stabilizer/additive in EU, USA, China and Japan, but residual amounts of egg-white lysozyme is considered sufficient to trigger allergic reactions in susceptible individuals, How to discover dosage-less and allergen-free of new source lysozyme are a big challenge. The new generation of LyoschTM G4 fermented lysozyme is produced by fermentation of bacterium Stretomyces globisporus cultured with sucrose and soybean peptone, that provides 10 - 15 times higher antibacterial activity against Clostridium causing cheese late-blowing and lactobacilli causing wine spoilage, when compared with egg-white lysozyme. LYSOCHTM G4 - FERTMENTED LYSOZYME Case 1: Gouda cheese Figure 1 shows that the effect of 15ppm LysochTM G4 has a stronger inhibition of clostridium tyrobutyricum than 150ppm egg-white lysozyme in Guda cheese. Clostridium tyrobutyricum 6 5 log /ml Butyric fermentation or “late blowing” in Gouda cheese is a ripening defect caused by Clostridium tyrobutyricum. Its growth leads to the formation of H2 and CO2 gases, cracks and slits in the cheese, and an abnormal aroma and cheese flavour. 4 3 2 0 2 4 6 Control 8 10 12 14 16 18 20 22 24 Hours 15ppm LysochTM G4 150ppm egg-white lysozyme Figure 1 Case 2: Red wine During winemaking, Lysozyme prevents the growth of spoilage lactic acid bacteria and controls malolactic fermentation (MLF), thus stabilizing the wine after alcoholic fermentation and/or MLF. Figure 1 shows that 15ppm LysochTM G4 has a similar control effect of Lacric acid bacteria as 150ppm egg-white lysozyme in red wine. TM Evolution of Lactic Acid Bacteria after MLF 60000 50000 40000 30000 20000 10000 150ppm eggwhite lysozyme Control Figure 2 [email protected] Tel: +32 23746314 Fax: +32 23747909 Avenue de Fré 267 1180 Brussels, Belgium www.handary.com O D N C OU C I L O R OP E TM HANDARY SA Day 21 Day 17 EU Freshcare for Quality More precisely, Handary is a leading producer in premium shelf-life extension ingredients, including Natap™ high-soluble natamycin, Epolyly™ ultrapure polylysine, NisinA® | NisinZ™ vegetal nisin A/Z, Lysoch™ fermented lysozyme, Proteria™ fermentates, Antimix™ antimicrobial blends, Chitoly™ fungi chitosan and chitin, Guardox™ natural antioxidants, Befresh™ fresh-keeper (enzyme, coating, culture) and Shelfex™ shelf-life extender. Day 7 F Handary is an industrial leader in delivering combined rapid microbial diagnostics and natural protective solutions to effectively increase food safety and shelf-life. Day 0 15ppm LysochTM G4 H A L AL F O 0
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