DBCO-PEG 30 kDa Cat. No. Amount CLK-A121-100 100 mg CLK-A121-1000 1g Description: A reagent for the PEGylation the azide-containing biomolecules via Copper-free click reaction. Important Product Information • Avoid buffers that contain azides, which can react with DBCO. • Reactions of DBCO and azides are more efficient at high concentrations and temperatures (i.e. 4 - 37 °C). Typical reaction times are less than 12 hours, however, incubating for longer can improve efficiency. Copper-free click reaction: • Prepare the azide-containing protein in reaction buffer. • Add DBCO-PEG conjugate to azide-containing sample. • Recommendation: Add 2 - 3 mol equivalents of DBCO-PEG conjugate to 1 mol equivalent of azide-containing protein. • Incubate the reaction mixture at room temperature for 4 - 12 hours for smaller DBCO-PEG (5, 10 kDa) and 12 - 24 hours for larger ones (20, 30, and 40 kDa). The incubation at 4 °C requires 6 24 hours. • The reaction mixture is now ready for purification by size exclusion chromatography if required. Structural formula of DBCO-PEG 30 kDa For research use only! Shipping: shipped at ambient temperature Storage Conditions: store at -20 °C Additional Storage Conditions: store undissolved, for use prepare a fresh solution Shelf Life: 12 months (undissolved) Molecular Weight: 30 kDa Purity: > 90 % (HPLC) Form: white to slightly grey solid Solubility: DCM, DMF, DMSO, water Jena Bioscience GmbH Löbstedter Str. 80 | 07749 Jena, Germany | Tel.:+49-3641-6285 000 | Fax:+49-3641-6285 100 http://www.jenabioscience.com Page 1 of 1 Last update: Apr 14, 2014
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