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Middle East Respiratory Syndrome (MERS) Coronavirus Vaccine and ELISA kits
MERS is a viral respiratory infection caused by the newly identified
MERS-coronavirus (MERS-CoV). MERS-CoV is a betacoronavirus
derived from bats. Camels have been shown to have antibodies to
MERS-CoV, but the exact source of infection in camels has not been
identified. A strain of MERS-CoV known as HCoV-EMC/2012 found in
the first patient in London in 2012 was found to have a 100% match to
Egyptian tomb bats.Early reportscompared the virus to severe acute
respiratory syndrome (SARS), and it has been referred to as Saudi
Arabia's SARS-like virus. ERS can range from asymptomatic disease
to severe pneumonia leading to the acute respiratory distress
syndrome. Renal failure, disseminated intravascular coagulation (DIC)
and pericarditis have also been reported. MERS have high fatality
rate, 77 deaths in 187 confirmed cases. As of May 2014, MERS-CoV
cases have been reported in several countries, including Saudi Arabia,
Malaysia, Jordan, Qatar, Egypt, the United Arab Emirates, Tunisia,
Kuwait, Oman, Algeria, Bangladesh, the Philippines (still MERS-free),
Indonesia (none was confirmed), the United Kingdom, and the United
States.MERS-CoV has been reported or by direct or indirect contact
withothers who have a travel history consistent with exposure in the
Middle East.However, the origin of the infection in most cases remains
unknown.Early research suggested the virus is related to one found in
the bats and in dromedary camels, as 90-100% camels have
antibodies to the MERS-CoV spike protein. Sera samples from
European sheep, goats, cattle, and other camelids had no such
antibodies. Countries like Saudi Arabia and the United Arab Emirates
produce and consume large amounts of camel meat. Human or
animals diagnostic serology is based upon PCR or ELISA or antibody
neutralization tests.
Coronaviruses are a
positive
ssRNA
genome of about 2732kb that codes for structural protein genes - namely the Spike (S),
Envelope (E), Membrane (M), and Nucleocapsid (N) genes - as well
as the Polymerase. The presence of MERS viral antibodies (N, E and
S) have been used to detect the infected animal or humans. Unlike
SARS-CoV, which uses human angiotensin-convertingenzyme 2
(ACE2) as its receptor for binding to ACE2-expressingcells, MERSCoV utilizes a different receptor, dipeptidylpeptidase 4 (DPP4), for
binding to DPP4-expressing cells via the Spike protein. S1 subunit
mediates virusbinding to cells expressing DPP4 through its receptorbindingdomain (RBD, 367-606 aa) region and an S2 subunit that
mediates virus-cell membrane fusion. A truncated RBD domain (377588)-Fc protein binds efficiently to DPP4. Antibodies to the RBD
domain also protected animals from MERS infection. Therefore,
MERS-CoV S1 region or the RBD are potential vaccine candidates.
The virus MERS-CoV is a new member of the beta group of
coronavirus, Betacoronavirus, lineage C. MERS-CoV genomes are
phylogenetically classified into two clades, clade A and B. The earliest
cases of MERS were of clade A clusters (EMC/2012 and JordanN3/2012), and new cases are genetically distinct (clade B).MERS-CoV
ADI has cloned and expressed various MERS recombinant proteins,
made antibodies and develop various ELISA kits to detect antibodies in
animals and humans.
ADI is the first company to introduce
commercial ELSIA kits to detect antibodies in camels, humans, or
other species.
is distinct from SARS and distinct from the
common-cold coronavirus and known endemic
human betacoronaviruses HCoV-OC43 and
HCoV-HKU1. MERS-CoV is more closely
related to the bat coronaviruses HKU4 and
HKU5 (lineage 2C) than it is to SARS-CoV
(lineage 2B) (2, 9), sharing more than 90%
sequence
identity
with
their
closest
relationships, bat coronaviruses HKU4 and
HKU5.
List of MERS ELISA Kits available from ADI.
Product details, data sheets, and pricing available (http://4adi.com/commerce/catalog/spcategory.jsp?category_id=2795
Items Description
RecombiVirus MERS
Nucleoprotein NP IgG
ELISA Kit
RecombiVirus MERS Spike
protein S1 IgG
ELISA Kit
RecombiVirus MERS Spike
protein S2 IgG
ELISA Kit
Kit
Type
MERS NP
antibody
MERS S1
antibody
MERS S2
antibody
ELISA Type IgG Cat#
Bat
Pig
Human
Camel
Cow
Goat/sheep
RV-402100-1
RV-402110-1
RV-402120-1
RV-402130-1
RV-402140-1
RV-402150-1
RV-402200-1
RV-402210-1
RV-402220-1
RV-402230-1
RV-402240-1
RV-402250-1
RV-402300-1
RV-402310-1
RV-402320-1
RV-402330-1
RV-402340-1
RV-402350-1
Notes: All of the ELISA kits are coated with purified recombinant proteins expressed in HEK or E. coli. There is no virus or viral
proteins used anywhere in the kit. So there is no risk of contamination in using these kits.
Alpha Diagnostic Intl Inc, USA;www.4adi.com; (800) 786-5777;[email protected]
List of MERS reagents available from ADI.
Items
MERS-NP
(HCov-EMC)
MERS-S1
(HCov-EMC
MERS-S1-RBD
(HCov-EMC
MERS-S2
(HCov-EMC
DPP4/CD26
Product
Type
Antibody
(mab)
Antibody
(pab)
Rec. Protein
Antibody
(pab)
Rec. Protein
Catalog#
Product Description
MERSNP11-M
Mouse monoclonal Anti-MERS-NP1 protein (1-413aa) IgG
MERSNP12-A
Rabbit anti-MERS-NP1 protein (1-413aa) IgG
MERSNP15-R-10
MERSS12-A
Recombinant (Sf9) Purified MERS NP (1-413aa, His-tag, ~47 kda, low Endotoxin, >95%)
Rabbit Anti-MERS-Spike protein S1 protein peptide, C-terminal IgG, aff pure
MERSS15-R-10
Rec. Protein
Rec. Protein
MERSS16-R-10
MERS35-R-10
Rec. Protein
MERS36-R-10
Rec. Protein
MERS37-R-10
Rec. Protein
MERS38-R-10
Antibody
(pab)
Antibody
(mab)
Rec. Protein
MERSS22-A
Recombinant (HEK) Purified MERS S1 (18-725 aa, His-tag, ~94 kda, low Endotoxin),
active
Recombinant (Sf9) Purified MERS S1 (18-725 aa, His-tag, ~94 kda, low Endotoxin), active
Recombinant (Sf9) Purified MERS RBD (367-606aa, Rb Fc-tag, ~51 kda, low Endotoxin),
active
Recombinant (Sf9) Purified MERS RBD (383-502aa, Rb Fc-tag, ~42 kda, low Endotoxin),
active
Recombinant (Sf9) Purified MERS RBD (367-606aa, His-tag, ~28 kda, low Endotoxin),
active
Recombinant (Sf9) Purified MERS RBD (383-502aa, Mouse Fc-tag, ~44 kda, low
Endotoxin), active
Rabbit Anti-MERS-Spike protein S2 protein (726-1296) IgG, aff pure
MERSS21-M
Mouse monoclonal Anti-MERS-Spike protein S2 protein (726-1296) IgG, aff pure
MERSS25-R-10
Recombinant (Sf9) Purified MERS S2 (726-1296 aa, His-tag, ~66 kda, low Endotoxin)
Antibody
(pab)
Rec. Protein
Rec. Protein
MERS121-A
Rabbit Anti-MERS-Spike protein (1-1297aa) IgG, aff pure
MERSS126-R-10
DPP45-R-10
Rec. Protein
DPP46-R-10
Rec. Protein
DPP47-R-10
Native
protein
Antibody
(pab)
DPP48-N-1
Recombinant (Sf9) Purified MERS S protein (1-1297 aa, His-tag, ~157 kda, low Endotoxin)
Recombinant (HEK) Purified Mouse DPP4 protein (29-760aa aa, hIgG1-Fc-tag, ~113 kda,
low Endotoxin)
Recombinant (HEK) Purified Human DPP4 protein (34-766aa aa, His-tag, ~87 kda, low
Endotoxin)
Recombinant (HEK) Purified Human DPP4 protein (29-766aa aa, hIgG-Fc-tag, ~125 kda,
low Endotoxin)
Purified Human PlacentaDipeptidyl Peptidase IV (DPP4), active
DPP41-A
Rabbit anti-human (mouse, rat) DPP4 peptide IgG, aff pure
Secondary Antibodies and Reagents related to MERS research
AntibodyType
Poly-Goat
Poly-Goat
Catalog# Product Description Quantity/SIZE
30816-A
Anti-Bat IgG (H+L chain) IgG, unconjugated, aff pure
30816-HP
Anti-Bat IgG (H+L chain) IgG-HRP Conjugate
Poly-Goat
Poly-Goat
Poly-Goat
Poly-Goat
Poly-Goat
30817-A
30817-APC
30817-B
30817-HP
30817-RPE
Anti-Llama IgG (H+L chain) IgG, unconjugated, aff pure
Anti-Llama IgG (H+L chain)-APC conjugate
Anti-Llama IgG (H+L chain)-Biotin conjugate
Anti-Llama IgG (H+L chain) IgG-HRP Conjugate
Anti-Llama IgG (H+L chain)-RPE conjugate
Rabbit-Poly
Rabbit-Poly
30818-RAF-BLK
30818-RAS-BLK
Llama IgG (H+L chain), Affinity Purified
Llama IgG (H+L chain), Antisera
Poly-Goat
Poly-Goat
Poly-Goat
Poly-Goat
Poly-Goat
Rabbit-Poly
Rabbit-Poly
Rabbit-Poly
Rabbit-Poly
Rabbit-Poly
Rabbit-Poly
Rabbit-Poly
Rabbit-Poly
30835-APC
30835-B
30835-HP
30835-RPE
30835-UL
30840-B
30840-F
30840-HP
30840-UL
30845-B
30845-F
30845-HP
30845-UL
Anti-Camel IgG-APC conjugate
Anti-Camel IgG-Biotin conjugate
Anti-Camel IgG-HRP Conjugate
Anti-Camel IgG-RPE conjugate
Anti-Camel IgG, aff pure, unlabeled
Anti-Llama IgG (heavy chain)-Biotin Conjugate
Anti-Llama IgG (heavy chain)-HRP Conjugate
Anti-Llama IgG (heavy chain), Affinity Purified
Anti-Llama IgG (heavy chain), Affinity Purified
Anti-Llama IgG (light chain)-Biotin Conjugate
Anti-Llama IgG (light chain)-HRP Conjugate
Anti-Llama IgG (light chain), Affinity Purified
Anti-Llama IgG (light chain), Affinity Purified
l
Alpha Diagnostic Intl Inc, USA;www.4adi.com; (800) 786-5777;[email protected]
MERS_Vaccine_ELISA-Flr
140728A
Alpha Diagnostic Intl Inc, USA;www.4adi.com; (800) 786-5777;[email protected]

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