濱崎万穂先生哺乳動物細胞におけるオートファゴソーム形成初期過程

バイオイメージングセンターセミナー
濱崎万穂先生
（大阪大学大学院医学系研究科遺伝医学講座・准教授／JSTさきがけ）
哺乳動物細胞における
オートファゴソーム形成初期過程の解析
日時：３月１２日木曜日１４時∼
場所：柏キャンパス生命棟地下１階講義室
要旨：
Autophagy is a tightly regulated intracellular degradation system that plays fundamental
roles in cellular homeostasis. Autophagy is induced by starvation, bacterial infection,
accumulation of aggregate proteins, organelle damages and so on. Autophagy is initiated by
nucleation of flattened vesicles termed isolation membranes that get elongated to engulf its
target and become autophagosomes. Autophagosomes then fuse with lysosomes to degrade
the contents. Autophagosomes are quite unique compared to other organelles as they form
only when they are required from the scratch. The site of this organelle formation and the
origin of their membranes have been a key question to understand the molecular
mechanism of autophagosome formation. In this study, we examined the initial stage of
autophagosome formation site. To determine the autophagosome formation site, we used
Atg14L (a component of initial autophagosome formation machinery) and Atg5 (an
autophagosome marker covers from the beginning) under starved condition. We observed
the ER-Mitochondria contact sites play an important role for the autophagosome
formation. This provides new insights into the autophagosome formation. In addition, we
will show the importance of autophagy in some diseases.
参考文献：
1. Hamasaki, M. et al. Up-to-date membrane biogenesis in the autophagosome formation.
Curr. Opin. Cell Biol. 25:455-60 (2013)
2. Hamasaki, M. et al. Autophagosomes form at ER-mitochondria contact sites. Nature,
495:389-93 (2013)
担当：鈴木邦律（生命応答システム分野）内線：63654

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