熊谷泉教授業績目録 - 東北大学

東北大学定年退職教員業績目録第 2012－28 号
熊谷
泉教授業績目録
平成２５年３月
東北大学史料館
生体機能化学講座（タンパク質工学分野）
熊谷泉
KUMAGAI Izumi
教授
大学院工学研究科 · 工学部バイオ工学専攻生体機能化学講座（タンパク質工学分野）
出身学校
東京大学・教養学部・基礎科学科
1972年卒業
出身大学院
東京大学・理学系研究科・相関理化学博士課程
1977年修了
取得学位
理学博士東京大学
1977年
略歴
1977年 — 1978年日本学術振興会奨励研究員
1978年 — 1980年三菱化成生命化学研究所特別研究員
1980年 — 1981年マックスプランク分子遺伝学研究所研究員
1981年 — 1983年ベルリン自由大学生物化学研究所研究員
1983年 — 1995年東京大学助手、講師、助教授（工学部）
東北大学教授（工学研究科）
1995年 — 現在
専門分野
遺伝子工学, 蛋白質工学, 機能生物化学
研究課題
・タンパク質の機能変換
・抗体の分子認識能の解析と変換
・ヒト抗体の応用研究
著書
1) 材料テクノロジー 24 生命体に学ぶ材料工学（執筆担当部分）バイオテクノロジー. [東京大学出版会,(1988)]
三浦謹一郎, 熊谷泉
2) 新酵素利用技術（執筆担当部分）タンパク質工学と酵素. [シーエムシー出版,(1988)]
熊谷泉
3) タンパク質工学-新しい物質生産の礎を築く（執筆担当部分）第 2 部基礎技術タンパク質化学. [啓学出版
,(1988)10 月]
熊谷泉
4) シリーズ分子生物学の進歩 3 蛋白質の機能構造（執筆担当部分）プロテアーゼとプロテアーゼインヒビター.
[丸善出版,(1990)]
熊谷泉, 竹内康男, 三井幸雄, 三浦謹一郎
5) プロテインエンジニアリング II（執筆担当部分）第 1 編タンパク質改変諸例第 1 章酵素の機能改変 1 酵素
の反応速度, 基質特異性の改変. [シーエムシー出版,(1990)]
熊谷泉
6) プロテインエンジニアリング II（執筆担当部分）第 1 編タンパク質改変諸例第 4 章その他のタンパク質の
機能変換-プロテアーゼ · インヒビター-. [シーエムシー出版,(1990)10 月]
小島修一, 熊谷泉
7) 機能性食品タンパク質工学ハンドブック（執筆担当部分）食品タンパク質のタンパク質工学. [サイエンス
フォーラム,(1991)1 月]
武田茂樹, 熊谷泉
8) 蛋白工学シリーズ蛋白工学における遺伝子発現の調節（執筆担当部分）遺伝子情報と発現 (遺伝子操作概論.
[講談社サイエンティフィク,(1991)5 月]
生体機能化学講座（タンパク質工学分野）
0 – 1
熊谷泉
熊谷泉, 百瀬春生
9) Maruzen Advanced Technology タンパク質工学-基礎と応用-（執筆担当部分）6 タンパク質の構造 · 機能相関
の解析. [丸善出版,(1992)7 月]
熊谷泉
10) 分子生物学からバイオテクノロジーへ（執筆担当部分）蛋白質工学と分子進化. [共立出版,(1993)]
熊谷泉
11) 機能性食品の研究（執筆担当部分）ニワトリリゾチームの高機能化と精密構造解析. [学会出版センター
,(1995)11 月]
熊谷泉
12) 7 遺伝子工学（執筆担当部分）生物工学基礎コース分子遺伝学. [丸善出版,(1997)3 月]
熊谷泉, 津本浩平
13) 天然高分子研究の新展開（執筆担当部分）抗体工学. [シーエムシー出版,(1998)]
熊谷泉, 津本浩平
14) 構造生物学（執筆担当部分）リゾチーム. [朝倉書店,(1998)1 月]
熊谷泉, 津本浩平, 前仲勝実
15) 生命工学-分子から環境まで（執筆担当部分）遺伝子工学概論. [(2000)12 月]
熊谷泉
16) シリーズ · ニューバイオフィジックス II-9 生体ナノマシンの分子設計（執筆担当部分）第 2 章 4 抗体の分子
設計. [共立出版,(2001)9 月]
津本浩平, 熊谷泉
17) 最新酵素利用技術と応用展開（執筆担当部分）酵素工学基礎編第 4 章タンパク質工学による酵素分子改質.
[シーエムシー出版,(2001)10 月]
津本浩平, 郷田秀一郎, 熊谷泉
18) 新タンパク質科学実験法第 3 巻構造機能解析 (上)（執筆担当部分）3.3 ディスプレイ法ファージディスプレ
イ. [化学同人,(2002)]
津本浩平, 熊谷泉
19) コンビナトリアルサイエンスの新展開（執筆担当部分）第 4 章ファージディスプレイを用いた新規能分子の
選択:抗体を中心に. [シーエムシー出版,(2002)2 月]
津本浩平, 熊谷泉
20) 化学フロンティアコンビナトリアル · バイオエンジニアリング（執筆担当部分）15 章特異性と親和性を両
立させた分子の人工創製を目指して. [化学同人,(2003)1 月]
津本浩平, 熊谷泉
21) ナノバイオ辞典（執筆担当部分）ファージディスプレイ、抗体. [テクノシステム,(2004)]
熊谷泉
22) 抗体エンジニアリングの最前線（執筆担当部分）第 1 章組換え型抗体の現状と展望. [シーエムシー出版,(2004)]
浅野竜太郎, 津本浩平, 熊谷泉
23) ゲノミクス · プロテオミクスの新展開 ∼ 生物情報の解析と応用 ∼ （執筆担当部分）ファージディスプレイ.
[エヌ · ティー · エス,(2004)4 月]
津本浩平, 熊谷泉
24) 生物物理学ハンドブック（執筆担当部分）抗体工学. [朝倉書店,(2004)4 月]
熊谷泉, 津本浩平
0 – 2
バイオ工学専攻
熊谷泉
25) コンビナトリアル · バイオエンジニアリングの最前線（執筆担当部分）ファージディスプレイの現状と今後：
プロテオミクスと蛋白質工学. [シーエムシー出版,(2004)8 月]
津本浩平, 熊谷泉
26) 蛋白質科学（執筆担当部分）抗体のファージディスプレイ · リボゾームディスプレイ. [化学同人,(2005)10 月]
熊谷泉
27) タンパク質の構造と機構. [医学出版,(2006)]
Alan Fersht 著, 桑島邦博, 有坂文雄, 熊谷泉, 倉光成紀訳
28) 抗体医薬の最前線（執筆担当部分）抗体ドメインの積み木細工による高機能化と医用への展開. [シーエムシー
出版,(2007)7 月]
熊谷泉, 浅野竜太郎, 中西猛
29) バイオ医薬の開発技術とシーズ（執筆担当部分）がん免疫療法への応用研究を目指した抗体-diabody-. [シー
エムシー出版,(2009)]
浅野竜太郎, 熊谷泉
30) バイオ医薬品製造の効率化と生産基材の開発（執筆担当部分）第Ⅲ編微生物を用いた製造第 5 章低分子治
療抗体の開発. [シーエムシー出版,(2012)4 月]
浅野竜太郎, 熊谷泉
31) 新機能抗体開発ハンドブック（執筆担当部分）第 1 編抗体開発技術-基礎第 2 章モノクローナル抗体 6 節
大量調製-微生物, 第 2 編抗体開発技術-実践第 2 章人工抗体 4 節二重特異性抗体. [エヌ · ティー · エス
,(2012)8 月]
浅野竜太郎, 熊谷泉
32) 次世代医薬開発に向けた抗体工学の最前線（執筆担当部分）巻頭言, 第 II 編抗体の改変技術第 7 章低分子
抗体 1 タンパク質工学を駆使した低分子抗体の高機能化, 第 II 編抗体の改変技術第 8 章その他新機能抗体
1 二重特異性がん治療抗体の開発. [シーエムシー出版,(2012)12 月]
熊谷泉監修
研究論文
1) Aromatic solvent-induced shift (ASIS). A novel approach. [Chem. Lett. (Jpn.), 1, (1972), 1239-1242]
Nakagawa N., Niki K., Takeuchi Y., Kumagai I.
2) Thermally induced biosynthesis of 2’-O-methylguanosine in tRNA from an extreme thermophile, Thermus
thermophilus HB27. [Proc.Natl.Acad.Sci.USA, 77 (4), (1980), 1922-1926]
Kumagai I., Watanabe K., Oshima T.
3) Isolation, puriﬁcation and some chemical properties of an acid carboxypeptidase from Aspergillus niger var.
macrospores. [Biochim.Biophys.Acta, 659 (2), (1981), 334-343]
Kumagai I., Yamasaki M., Ui N.
4) Enzymatic properties of an acid carboxypeptidase from Aspergillus niger var. macrospores. [Biochim.Biophys.Acta,
659 (2), (1981), 344-350]
Kumagai I., Yamasaki M.
5) The nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8. [Nucleic
Acids Res., 9 (19), (1981), 5159-5162]
Kumagai I., Digweed M., Erdmann V.A., Watanabe K., Oshima T.
6) Phylogenetic diversity reﬂected in the three dimensional structure of ribosomal 5S RNA. [Zbl. Bakt. Hyg., I.
Abt. Orig., 3 (1), (1982), 69-78]
Pieler T., Kumagai I., Erdmann V.
生体機能化学講座（タンパク質工学分野）
0 – 3
熊谷泉
7) A thermostable tRNA (guanosine-2’)-methyltransferase from Thermus thermophilus HB27 and the eﬀect of
ribose methylation on the conformational stability of tRNA. [J.Biol.Chem., 257 (13), (1982), 7388-7395]
Kumagai I., Watanabe K., Oshima T.
8) The eﬀect of a cytidine-to-uridine transition on the stability of Escherichia coli A19 5-S RNA. [Eur. J.
Biochem., 127 (3), (1982), 531-537]
Digweed M., Kumagai I., Pieler T., Erdmann V.A.
9) Nucleotide sequence and secondary structure analysis of spinach chloroplast 4.5 S RNA. [J. Biol. Chem., 257
(21), (1982), 12924-12928]
Kumagai I., Pieler T., Subramanian A.R., Erdmann V.A.
10) Terminal heterogeneity and corrections of the nucleotide sequence of 5S rRNA from an extreme thermophile,
Thermus thermophilus HB8. [Nucleic Acids Res., 11 (4), (1983), 913-916]
Komiya H., Kawakami M., Takemura S., Kumagai I., Erdmann V.A.
11) Chemical accessibility of the 4.5S RNA in spinach chloroplast ribosomes. [Nucleic Acids Res., 11 (4), (1983),
961-970]
Kumagai I., Bartsch M., Subramanian A.R., Erdmann V.A.
12) The number of ribosomal RNA genes in Thermus thermophilus HB8. [Nucleic Acids Res., 12 (4), (1984),
2055-2060]
Ulbrich N., Kumagai I., Erdmann V.A.
13) A new method for the synthesis of oligodeoxyribonucleotides bearing a 5’-terminal phosphate group. [Chem.
Lett. (Jpn.), 16 (1), (1987), 117-120]
Hata T., Kamimura T., Urakami K., Kohno K., Sekine M., Kumagai I., Shinozaki K., Miura K.
14) Unusual genetic codes and a novel gene structure for tRNA(SerAGY) in starﬁsh mitochondrial DNA. [Gene,
56, (1987), 219-230]
Himeno H., Masaki H., Kawai T., Ohta T., Kumagai I., Miura K., Watanabe K.
15) Molecular cloning and sequencing of cDNA encoding goat pre α-lactalbumin. [J. Biochem. (Tokyo), 101 (2),
(1987), 511-517]
Kumagai I., Tamaki E., Kakinuma S., Miura K.
16) A thermostable Gm-methylase recognizes the tertiary structure of tRNA. [J. Biochem. (Tokyo), 101 (5),
(1987), 1191-1198]
Matsumoto T., Ohta T., Kumagai I., Oshima T., Murao K., Hasegawa T., Ishikura H., Watanabe K.
17) Conversion of Trp 62 of hen egg-white lysozyme to Tyr by site-directed mutagenesis. [J. Biochem. (Tokyo),
102 (4), (1987), 733-740]
Kumagai I., Kojima S., Tamaki E., Miura K.
18) Partial alteration of a protein Streptomyces subtilisin inhibitor by site-directed mutagenesis. [Proceed. Jpn.
Acad. Ser. B. Phys. Biol. Sci., 64 (6), (1988), 147-149]
Miura K., Kumagai I., Obata S., Kojima S., Taguchi S.
19) Alteration of substrate preference of hen egg-white lysozyme by conversion of Trp 62 using site-directed
mutagenesis. [The Immune Response to Structurally Deﬁned Proteins: The Lysozyme Model, (1989), 51-57]
Kumagai I., Miura K.
20) Studies on chemical synthesis of human cystatin A gene and its expression in Escherichia coli. [J. Biochem.
(Tokyo), 105 (1), (1989), 143-147]
Kaji H., Kumagai I., Takeda A., Miura K., Samejima T.
21) Molecular cloning and nucleotide sequence determination of gene encoding Streptomyces subtilisin inhibitor
(SSI). [J. Biochem. (Tokyo), 105 (3), (1989), 367-371]
0 – 4
バイオ工学専攻
熊谷泉
Obata S., Taguchi S., Kumagai I., Miura K.
22) High-level expression in Streptomyces lividans 66 of a gene encoding Streptomyces subtilisin inhibitor from
Streptomyces albogriseolus S-3253. [J. Biochem. (Tokyo), 105 (3), (1989), 372-376]
Obata S., Furukubo S., Kumagai I., Takahashi H., Miura K.
23) Enhanced bacteriolytic activity of hen egg-white lysozyme due to conversion of Trp62 to other aromatic amino
acid residues. [J. Biochem. (Tokyo), 105 (6), (1989), 946-948]
Kumagai I., Miura K.
24) Chemical synthesis of a gene for human cysteine proteinase inhibitor, cystain A, and its expression in E. coli.
[Intracellular Proteolysis-Mechanism and Recognition, (1989), 313-320]
Kaji H., Samejima T., Takeda A., Kumagai I., Miura K.
25) Eﬃcient extracellular expression of a foreign protein in Streptomyces using secretory protease inhibitor (SSI)
gene fusions. [Bio/technology (N. Y.), 7 (10), (1989), 1063-1066]
Taguchi S., Kumagai I., Nakayama J., Suzuki A., Miura K.
26) Eﬀects of modiﬁcation of 4-thiouridine in E. coli tRNA(fMet) on its methyl acceptor activity by thermostable
Gm-methylases. [J. Biochem. (Tokyo), 106 (5), (1989), 798-802]
Hori H., Saneyoshi M., Kumagai I., Miura K., Watanabe K.
27) Analysis of transcriptional control regions in the Streptomyces subtilisin-inhibitor-encoding gene. [Gene, 84
(2), (1989), 279-286]
Taguchi S., Nishiyama K., Kumagai I., Miura K.
28) Molecular recognition at the interface between subtilisin BPN’ and its proteinaceous inhibitor, SSI. [Protein
Eng., (1990), 103-108]
Takeuchi Y., Noguchi S., Satow Y., Kojima S., Kumagai I., Miura K., Mitsui Y.
29) Alteration of the functional structure of the protease inhibitor, Streptomyces subtilisin inhibitor by site-directed
mutagenesis. [Protein Eng., (1990), 147-152]
Kojima S., Furukubo S., Nishiyama Y., Fujimura K., Kumagai I., Miura K.
30) Expression of goat alpha-lactalbumin in Escherichia coli and its refolding to biologically active protein. [Protein
Eng., 3 (5), (1990), 449-452]
Kumagai I., Takeda S., Hibino T., Miura K.
31) Eﬀect on inhibitory activity of mutation at reaction site P4 of the Streptomyces subtilisin inhibitor, SSI. [Protein
Eng., 3 (6), (1990), 527-530]
Kojima S., Kumagai I., Miura K.
32) Alteration of the speciﬁcity of the Streptomyces subtilisin inhibitor by gene engineering. [Bio/technology (N.
Y.), 8 (5), (1990), 449-452]
Kojima S., Obata S., Kumagai I., Miura K.
33) Eﬃcient preparation of human recombinant cystatin A by Escherichia coli. [Biol. Chem. Hoppe Seyler, 371,
(1990), 145-150]
Kaji H., Samejima T., Kumagai I., Hibino T., Miura K., Takeda A.
34) Comparison of secretory expression in Escherichia coli and Streptomyces of Streptomyces subtilisin inhibitor
(SSI) gene. [Biochim. Biophys. Acta, 1049 (3), (1990), 278-285]
Taguchi S., Kumagai I., Miura K.
35) Expression and secretion of bovine β-lactoglobulin in Saccharomyces cerevisiae. [Agric.Biol.Chem., 54 (12),
(1990), 3111-3116]
Totsuka M., Katakura Y., Shimizu M., Kumagai I., Miura K., Kaminogawa S.
生体機能化学講座（タンパク質工学分野）
0 – 5
熊谷泉
36) Expression and secretion of goat α-lactalbumin as an active protein in Saccharomyces cerevisiae. [Biochem.
Biophys. Res. Commun., 173 (2), (1990), 741-747]
Takeda S., Tamaki E., Miura K., Kumagai I.
37) Introduction of enzymatic-activity to α-lactalbumin by artiﬁcial exon shuﬄing. [Proceed. Jpn. Acad. Ser. B.
Phys. Biol. Sci., 67, (1991), 184-187]
Kumagai I., Takeda S., Miura K.
38) Inhibition of subtilisin BPN’ by reaction site P1 mutants of Streptomyces subtilisin inhibitor. [J. Biochem.
(Tokyo), 109 (3), (1991), 377-382]
Kojima S., Nishiyama Y., Kumagai I., Miura K.
39) Mechanisms of temporary inhibition in Streptomyces subtilisin inhibitor induced by an amino acid substitution,
tryptophan 86 replaced by histidine. [Biochemistry, 30 (21), (1991), 5275-5286]
Tamura A., Kanaori K., Kojima S., Kumagai I.
40) Relationship between utilization of dual translational initiation signals and protein processing in Streptomyces.
[Mol.Gen.Gent., 226 (1-2), (1991), 328-331]
Taguchi S., Nishiyama K., Kumagai I., Momose H., Miura K.
41) Molecular recognition at the active site of subtilisin BPN’: crystallographic studies using genetically engineered
proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor). [Protein Eng., 4 (5), (1991), 501-508]
Takeuchi Y., Noguchi S., Satow Y., Kojima S., Kumagai I., Miura K., Nakamura K.T., Mitsui Y.
42) Redesign of the substrate-binding site of hen egg white lysozyme based on the molecular evolution of C-type
lysozymes. [J. Biol. Chem., 267 (7), (1992), 4608-4612]
Kumagai I., Sunada F., Takeda S., Miura K.
43) Functional conversion of the homologous proteins α-lactalbumin and lysozyme by exon exchange. [Proc. Natl.
Acad. Sci. USA, 89 (13), (1992), 5887-5891]
Kumagai I., Takeda S., Miura K.
44) A protease inhibitor produced by Streptomyceslividans 66 exhibits inhibitory activities toward both subtilisin
BPN’ and trypsin. [J. Biochem. (Tokyo), 112 (2), (1992), 204-211]
Ueda Y., Kojima S., Tsumoto K., Takeda S., Miura K., Kumagai I.
45) The eﬀects of a unique D-loop structure of a minor tRNA(LeuUUA) from Streptomyces on its structural stability
and amino acid accepting activity. [Nucleic Acids Res., 20 (15), (1992), 3911-3917]
Ueda Y., Kumagai I., Miura K.
46) Structural and functional properties of hen egg-white lysozyme deamidated by protein engineering.
[Biosci.Biotech.Biochem., 56 (9), (1992), 1424-1428]
Kato A., Tanimoto S., Muraki Y., Kobayashi K., Kumagai I.
47) Isolation and partial characterization of SSI-like protease inhibitors from Streptomyces. [FEMS Microbiol.
Lett., 78 (2-3), (1992), 293-297]
Taguchi S., Kojima S., Kumagai I., Ogawara H., Miura K., Momose H.
48) The primary structure of pre-cytochrome C3 from Desulfovibirio vulgaris (Miyazaki F) as determined by
nucleotide sequencing of its gene and partial amino acid sequencing. [Protein Seq. Data Anal., 5 (5), (1993),
193-196]
Kitamura M., Ozawa K., Kojima S., Kumagai I., Akutsu H., Miura K.
49) Eﬀects of subsite alterations on substrate-binding mode in the active site of hen egg-white lysozyme. [Eur. J.
Biochem., 212 (1), (1993), 151-156]
Kumagai I., Maenaka K., Sunada F., Takeda S., Miura K.
50) High frequency of SSI-like protease inhibitors among Streptomyces. [Biosci.Biotech.Biochem., 57 (3), (1993),
522-524]
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Taguchi S., Kikuchi H., Kojima S., Kumagai I., Nakase T., Miura K., Momose H.
51) Eﬀect of downstream message secondary structure on the secretory expression of the Streptomyces subtilisin
inhibitor. [FEMS Microbiol. Lett., 107 (2-3), (1993), 185-190]
Taguchi S., Yoshida Y., Kumagai I., Miura K., Momose H.
52) Eﬀect of a rare leucine codon, TTA, on expression of a foreign gene in Streptomyces lividans.
[Biochim.Biophys.Acta, 1172 (3), (1993), 262-266]
Ueda Y., Taguchi S., Nishiyama K., Kumagai I., Miura K.
53) Requirement for a disulﬁde bridge near the reactive site of protease inhibitor SSI (Streptomyces subtilisin
inhibitor) for its inhibitory action. [J. Mol. Biol., 230 (2), (1993), 395-399]
Kojima S., Kumagai I., Miura K.
54) Eﬀect of tandem repeated AUG codons on translation eﬃciency of eukaryotic mRNA carrying a short leader
sequence. [Mol.Gen.Gent, 238 (1-2), (1993), 59-64]
Wakiyama M., Hirao I., Kumagai I., Miura K.
55) Eﬀects of deletion in the ﬂexible loop of the protease inhibitor SSI (Streptomyces subtilisin inhibitor) on
interactions with proteases. [Protein Eng., 6 (3), (1993), 297-303]
Kojima S., Furukubo S., Kumagai I., Miura K.
56) Synthesis and expression of a DNA encoding the Fv domain of an anti-lysozyme monoclonal antibody,
HyHEL10, in Streptomyces lividans. [Gene, 129 (1), (1993), 129-134]
Ueda Y., Tsumoto K., Watanabe K., Kumagai I.
57) Interaction of subtilisin BPN’ and recombinant Streptomyces subtilisin inhibitors with substituted P1 site
residues. [J. Biochem. (Tokyo), 114 (4), (1993), 553-559]
Masuda-Momma K., Hatanaka T., Inouye K., Kanaori K., Tamura A., Akasaka K., Kojima S., Kumagai
I., Miura K., Tonomura B.
58) Identiﬁcation of amino acid residues responsible for the changes of absorption and ﬂuorescence spectra on the
binding of subtilisin BPN’ and Streptomyces subtilisin inhibitor. [J. Biochem. (Tokyo), 114 (6), (1993),
906-911]
Masuda-Momma K., Shimakawa T., Inouye K., Hiromi K., Kojima S., Kumagai I., Miura K., Tonomura
B.
59) Contribution of salt bridge in the protease inhibitor SSI (Streptomyces subtilisin inhibitor) to its inhibitory
action. [FEBS Lett., 337 (2), (1994), 195-199]
Kojima S., Fujimura K., Kumagai I., Miura K.
60) High-level expression of porcine muscle adenylate kinase in Escherichia coli: eﬀects of the copy number of the
gene and the translational initiation signals. [J. Biotechnol., 32 (2), (1994), 139-148]
Hibino T., Misawa S., Wakiyama M., Maeda S., Yazaki K., Kumagai I., Ooi T., Miura K.
61) Novel FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F). Cloning and expression of its gene in
Escherichia coli. [J. Biol. Chem., 269 (8), (1994), 5566-5573]
Kitamura M., Kojima S., Ogasawara K., Nakaya T., Sagara T., Niki K., Miura K., Akutsu H., Kumagai
I.
62) Functional and structural role of a tryptophan generally observed in protein-carbohydrate interaction. Trp-62
of hen egg white lysozyme. [J. Biol. Chem., 269 (10), (1994), 7070-7075]
Maenaka K., Kawai G., Watanabe K., Sunada F., Kumagai I.
63) Substrate speciﬁcity of tRNA (adenine-1-)-methyltransferase from Thermus thermophilus HB27. [Biosci.
Biotechnol. Biochem., 58 (6), (1994), 1128-1133]
Yamazaki N., Hori H., Ozawa K., Nakanishi S., Ueda T., Kumagai I., Watanabe K., Nishikawa K.
生体機能化学講座（タンパク質工学分野）
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64) Secretory production of chicken ovomucoid domain 3 by Escherichia coli and alteration of inhibitory speciﬁcity
toward proteases by substitution of the P1 site residue. [Gene, 143 (2), (1994), 239-243]
Kojima S., Fushimi N., Ikeda A., Kumagai I., Miura K.
65) Eﬀect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in
E. coli and the thermodynamic analysis of its antigen-binding properties. [Biochem. Biophys. Res. Commun.,
201 (5), (1994), 546-551]
Tsumoto K., Nakaoki Y., Ueda Y., Ogasahara K., Yutani K., Watanabe K., Kumagai I.
66) Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody
binding. [J. Biol. Chem., 269 (46), (1994), 28777-28782]
Tsumoto K., Ueda Y., Maenaka K., Watanabe K., Ogasahara K., Yutani K., Kumagai I.
67) A gene encoding a cytochrome c oxidase-like protein is located closely to the cytochrome c-553 gene in the
anaerobic bacterium, Desulfovibrio vulgaris (Miyazaki F). [Microbiol Immunol, 39 (1), (1995), 75-80]
Kitamura M., Mizugai K., Taniguchi M., Akutsu H., Kumagai I., Nakaya T.
68) Dissection of protein-carbohydrate interactions in mutant hen egg-white lysozyme complexes and their
hydrolytic activity. [J. Mol. Biol., 247 (2), (1995), 281-293]
Maenaka K., Matsushima M., Song H., Sunada F., Watanabe K., Kumagai I.
69) Human cystatin A is inactivated by engineered truncation. The NH2-terminal region of the cysteine proteinase
inhibitor is essential for expression of its inhibitory activity. [Biochemistry, 34 (38), (1995), 12185-12192]
Shibuya K., Kaji H., Itoh T., Ohyama Y., Tsujikami A., Tate S., Takeda A., Kumagai I
70) Role of Tyr residues in the contact region of anti-lysozyme monoclonal antibody HyHEL10 for antigen binding.
[J. Biol. Chem., 270 (31), (1995), 18551-18557]
Tsumoto K., Ogasahara K., Ueda Y., Watanabe K., Yutani K., Kumagai I.
71) Eﬀects of amino acid substitutions in the hydrophobic core of α-lactalbumin on the stability of the molten
globule state. [Protein Eng., 8 (11), (1995), 1153-1161]
Uchiyama H., Perez-Prat E.M., Watanabe K., Kumagai I., Kuwajima K.
72) A stable phage-display system using a phagemid vector: phage display of hen egg-white lysozyme (HEL),
Escherichia coli alkalinephosphatase, and anti-HEL monoclonal antibody, HyHEL10. [Biochem. Biophys.
Res. Commun., 218 (3), (1996), 682-687]
Maenaka K., Furuta M., Tsumoto K., Watanabe K., Ueda Y., Kumagai I.
73) Synthesis of α-helix-forming peptides by gene engineering methods and their characterization by circular
dichroism spectra measurements. [Biochim. Biophy. Acta., 1294 (2), (1996), 129-137]
Kojima S., Kuriki Y., Sato Y., Arisaka F., Kumagai I., Takahashi S., Miura K.
74) Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region.
[Nat. Biotechnol., 14 (13), (1996), 1714-1718]
Ueda H., Tsumoto K., Kubota K., Suzuki E., Nagamune T., Nishimura H., Schueler P.A., Winter G.,
Kumagai I.
75) Study of antibody-antigen interaction through site-directed mutagenesis of the VH region of a hybrid phageantibody fragment. [Protein Eng., 9 (12), (1996), 1211-1217]
Li Y., Owen M.R., Cockburn W., Kumagai I., Whitelam G.C.
76) Role of salt bridge formation in antigen-antibody interaction. Entropic contribution to the complex between hen
egg-white lysozyme and its monoclonal antibody HyHEL10. [J.Biol. Chem., 271 (51), (1996), 32612-32616]
Tsumoto K., Ogasahara K., Ueda Y., Watanabe K., Yutani K., Kumagai I.
77) Cloning and expression of the rubredoxin gene from Desulfovibrio vulgaris (Miyazaki F) - Comparison of the
primary structure of desulfoferrodoxin. [Biochim. Biophys. Acta, 1351 (1-2), (1997), 239-247]
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Kitamura M., Koshino Y., Kamikawa Y., Kohno K., Kojima S., Miura K., Sagara T., Akutsu H., Kumagai
I., Nakaya T.
78) Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the
presence of antigen. [Protein Eng., 10 (11), (1997), 1311-1318]
Tsumoto K., Nishimiya Y., Kasai N., Ueda H., Nagamune T., Ogasahara K., Yutani K., Tokuhisa K.,
Matsushima M., Kumagai I.
79) Substrate recognition of tRNA (Guanosine-2’-)-methyltransferase from Thermus thermophilus HB27. [J. Biol.
Chem., 2 (2), (1998), 147-154]
Kitamura M., Konishi T., Kawanishi K., Ohashi K., Kishida Y., Kohno K., Akutsu H., Kumagai I., Nakaya
T.
80) Construction of mono- and bivalent human single-chain Fv fragments against the D antigen in the Rh blood
group: multimerization eﬀect on cell agglutination and application to blood typing. [Protein Eng., 11 (3),
(1998), 233-241]
Furuta M., Uchikawa M., Ueda Y., Yabe T., Taima T., Tsumoto K., Kojima S., Juji T., Kumagai I.
81) Structural analysis of mutant hen egg-white lysozyme preferring a minor binding mode. [Biochim. Biophys.
Acta, 1384 (1), (1998), 23-31]
Maenaka K., Matsushima M., Kawai G., Watanabe K., Kuroki R., Kumagai I.
82) Cloning and expression of the gene encoding ﬂavodoxin from Desulfovibrio vulgaris (Miyazaki F). [J. Biochem.
( Tokyo ), 123 (5), (1998), 891-898]
Kitamura M., Sagara T., Taniguchi M., Ashida M., Ezoe K., Kohno K., Kojima S., Ozawa K., Akutsu H.,
Kumagai I., Nakaya T.
83) Structural and functional eﬀect of Trp-62–> Gly and Asp-101–> Gly substitutions on substrate-binding modes
of mutant hen egg-white lysozymes. [Biochem. J., 333 (Pt1), (1998), 71-76]
Maenaka K., Matsushima M., Kawai G., Kidera A., Watanabe K., Kuroki R., Kumagai I.
84) Calorimetric study of mutant human lysozymes with partially introduced Ca 2+ binding sites and its eﬃcient
refolding system from inclusion bodies. [Protein Eng., 11 (8), (1998), 683-690]
Koshiba T., Tsumoto K., Masaki K., Kawano K., Nitta K., Kumagai I.
85) P1 speciﬁcity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1substituted derivatives of Streptomyces subtilisin inhibitor. [Biosci. Biotechnol. Biochem., 62 (10), (1998),
2035-2038]
Tanaka T., Matsuzawa H., Kojima S., Kumagai I., Miura K., Ohta T.
86) Highly eﬃcient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in
Escherichia coli by controlled introduction of oxidizing reagent-application to a human single-chain Fv fragment.
[J. Immunol. Meth, 219 (1-2), (1998), 119-129]
Tsumoto K., Shinoki K., Kondo H., Uchikawa M., Juji T., Kumagai I.
87) Substrate recognition of tRNA (Guanosine-2’-)-methyltransferase from Thermus thermophilus HB27. [J. Biol.
Chem., 273 (40), (1998), 25721-25727]
Hori H., Yamazaki N., Matsumoto T., Watanabe Y., Ueda T., Nishikawa K., Kumagai I., Watanabe K.
88) Refolding of therapeutic proteins produced in Escherichia coli as inclusion bodies. [Biopolymers, 51 (4),
(1999), 297-307]
Misawa S., Kumagai I.
89) Eﬀect of the extra N-terminal methionine residue on the stability and folding of recombinant α-lactalbumin
expressed in Escherichia coli. [J. Mol. Biol., 285 (3), (1999), 1179-1194]
Chaudhuri T.K., Horii K., Yoda T., Arai M., Nagata S., Terada T.P., Uchiyama H., Ikura T., Tsumoto
K., Kataoka H., Matsushima M., Kuwajima K., Kumagai I.
生体機能化学講座（タンパク質工学分野）
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90) Cell-free expression of two single-chain monoclonal antibodies against lysozyme: eﬀect of domain arrangement
on the expression. [J. Biochem. ( Tokyo ), 125 (2), (1999), 328-333]
Merk H., Stiege W., Tsumoto K., Kumagai I., Erdmann V.A.
91) SEA-scFv as a bifunctional antibody: construction of a bacterial expression system and its functional analysis.
[Biochem. Biophys. Res. Commun., 256 (1), (1999), 223-230]
Sakurai N., Kudo T., Suzuki M., Tsumoto K., Takemura S., Kodama H., Ebara S., Teramae A., Katayose
Y., Shinoda M., Kurokawa T., Hinoda Y., Imai K., Matsuno S., Kumagai I.
92) Expression of a model peptide of a marine mussel adhesive protein in Escherichia coli and characterization of
its structural and functional properties. [J. Polymer Sci. Part A. Polymer Chem., 37 (6), (1999), 729-736]
Kitamura M., Kawakami K., Nakamura N., Tsumoto K., Uchiyama H., Ueda Y., Kumagai I., Nakaya T.
93) Open sandwich ELISA with V(H)-/V(L)-alkaline phosphatase fusion proteins. [J. Immunol. Meth., 224 (1-2),
(1999), 171-184]
Suzuki C., Ueda H., Tsumoto K., Mahoney W.C., Kumagai I., Nagamune T.
94) Expression of a synthetic gene encoding canine milk lysozyme in Escherichia coli and characterization of the
expressed protein. [Protein Eng., 12 (5), (1999), 429-435]
Koshiba T., Hayashi T., Miwako I., Kumagai I., Ikura T., Kawano K., Nitta K., Kuwajima K.
95) Crystal structure of anti-Hen egg white lysozyme antibody (HyHEL-10) Fv-antigen complex. Local structural
changes in the protein antigen and water-mediated interactions of Fv-antigen and light chain-heavy chain
interfaces. [J. Biol. Chem., 274 (39), (1999), 27623-27631]
Kondo H., Shiroishi M., Matsushima M., Tsumoto K., Kumagai I.
96) Homogeneous noncompetitive immunoassay based on the energy transfer between ﬂuorolabeled antibody
variable domains (open sandwich ﬂuoroimmunoassay). [Biotechniques, 27 (4), (1999), 738-742]
Ueda H., Kubota K., Wang Y., Tsumoto K., Mahoney W., Kumagai I.
97) Kinetic folding reactions and molecular dynamics simulations of α-lactalbumin. [Old and New Views of
Protein Folding (Proc. of 24th Taniguchi Symposium), (1999), 155-161]
Yoda T., Saito M., Arai M., Horii K., Tsumoto K., Matsushima M., Kumagai I.
98) Functional Construction of Anti-Mucin Core Protein (MUC1) Antibody MUSE11 Variable Regions in a
Bacterial Expression System. [J. Biochem., 127 (4), (2000), 673-679]
Asano R., Takemura S., Tsumoto K., Sakurai N., Teramae A., Ebara S., Katayose Y., Shinoda M., Suzuki
M., Imai K., Matsuno S., Kudo T., Kumagai I.
99) Thermodynamic consequences of grafting enhanced aﬃnity toward the mutated antigen onto an antibody. The
case of anti-lysozyme antibody, HyHEL-10. [ J. Biol. Chem., 275 (17), (2000), 12813-12820]
Nishimiya Y., Tsumoto K., Shiroishi M., Yutani K., Kumagai I.
100) Fluorolabeling of antibody variable domains with green ﬂuorescent protein variants: application to an energy
transfer-based homogeneous immunoassay. [Protein Eng., 13 (5), (2000), 369-376]
Arai R., Ueda H., Tsumoto K., Mahoney W.C., Kumagai I., Nagamune T.
101) Isolation and characterization of monoclonal antibodies that inhibit hepatitis C virus NS3 protease. [J. Virol.,
74 (14), (2000), 6300-6308]
Ueno T., Misawa S., Ohba Y., Matsumoto M., Mizunuma M., Kasai N., Tsumoto K., Kumagai I., Hayashi
H.
102) Functional Fv fragment of an antibody speciﬁc for CD28:Fv-mediated co-stimulation of T cells. [FEBS Lett.,
476 (3), (2000), 266-271]
Takemura S., Asano R., Tsumoto K., Arai T., Sakurai N., Kodama H., Yoshida H., Katayose Y., Suzuki
M., Matsuno S., Kudo T., Kumagai I.
103) Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-speciﬁc Ig V-domains fused
to truncated Epo receptor. [J. Immunol. Meth., 241 (1-2), (2000), 159-170]
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Ueda H., Kawahara M., Aburatani T., Tsumoto K., Todokoro K., Suzuki E., Nishimura H., Schueler P.A.,
Winter G., Mahoney W.C., Kumagai I., Nagamune T.
104) Construction of a diabody (small recombinant bispeciﬁc antibody) using a refolding system. [Protein Eng., 13
(8), (2000), 583-588]
Takemura S., Asano R., Tsumoto K., Sakurai N., Katayose Y., Kodama H., Ebara S., Suzuki M., Imai
K., Matsuno S., Kudo T., Kumagai I.
105) Thermodynamic Consequences of Single-mutation on Association of an Antibody with Its Speciﬁc Antigen:
The Case of HyHEL-10-hen Lysozyme Complex. [Chem. Lett. (Jpn.), 29 (9), (2000), 1066-1067]
Tsumoto K., Kumagai I.
106) Folding-unfolding of goat α-lactalbumin studied by stopped-ﬂow circular dichroism and molecular dynamics
simulations. [Proteins, 42 (1), (2001), 49-65]
Yoda T., Saito M., Arai M., Horii K., Tsumoto K., Matsushima M., Kumagai I., Kuwajima K.
107) Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library.
[J. Biochem. (Tokyo), 129 (2), (2001), 209-212]
Nakamura M., Watanabe H., Nishimiya Y., Tsumoto K., Ishimura K., Kumagai I.
108) Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy
transfer. [Anal. Biochem., 298 (1), (2001), 77-81]
Arai R., Nakagawa H., Tsumoto K., Mahoney W., Kumagai I., Ueda H., Nagamune T.
109) Inhibition of the hepatitis C virus NS3 protease activity by Fv fragment of antibody 8D4. [Biochem. Biophys.
Res. Commun., 281 (2), (2001), 416-424]
Kasai N., Tsumoto K., Niwa S., Misawa S., Ueno T., Hayashi H., Kumagai I.
110) Cloning and expression of the catalase gene from the anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F).
[J. Biochem. (Tokyo), 129 (3), (2001), 357-364]
Kitamura M., Nakanishi T., Kojima S., Kumagai I., Inoue H.
111) Structural evidence for entropic contribution of salt bridge formation to a protein antigen-antibody interaction:
the case of hen lysozyme-HyHEL-10 Fv complex. [J. Biol. Chem., 276 (25), (2001), 23042-23050]
Shiroishi M., Yokota A., Tsumoto K., Kondo H., Nishimiya Y., Horii K., Matsushima M., Ogasahara K.,
Yutani K., Kumagai I.
112) A growth signal with an artiﬁcially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer.
[J. Biochem., 130 (2), (2001), 305-312]
Kawahara M., Ueda H., Tsumoto K., Kumagai I., Mahoney W., Nagamune T.
113) Replacing factor-dependency with that for lysozyme: aﬀordable culture of IL-6-dependent hybridoma by
transfecting artiﬁcial cell surface receptor. [Biotechnol. Bioeng., 74 (5), (2001), 416-423]
Kawahara M., Natsume A., Terada S., Kato K., Tsumoto K., Kumagai I., Miki M., Mahoney W., Ueda
H., Nagamune T.
114) Contribution of Thr29 to the thermodynamic stability of goat α-lactalbumin as determined by experimental
and theoretical approaches. [Proteins, 45 (1), (2001), 16-29]
Horii K., Saito M., Yoda T., Tsumoto K., Matsushima M., Kuwajima K., Kumagai I.
115) A visualization method of ﬁlamentous phage infection and phage-derived proteins in Escherichia coli using
biotinylated phages. [Biochem. Biophys. Res. Commun., 289 (1), (2001), 252-256]
Nakamura M., Tsumoto K., Ishimura K., Kumagai I.
116) Mutated SEA-D227A-conjugated antibodies greatly enhance antitumor activity against MUC1-expressing bile
duct carcinoma. [Cancer Immunol. Immunother., 50 (10), (2001), 539-548]
Kodama H., Suzuki M., Katayose Y., Shinoda M., Sakurai N., Takemura S., Yoshida H., Saeki H., Ichiyama
M., Tsumoto K., Asano R., Kumagai I., Imai K., Hinoda Y., Matsuno S., Kudo T.
生体機能化学講座（タンパク質工学分野）
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117) A mutated superantigen SEA D227A fusion diabody speciﬁc to MUC1 and CD3 in targeted cancer
immunotherapy for bile duct carcinoma. [Cancer Immunol. Immunother., 51 (1), (2002), 33-44]
Takemura S., Kudo T., Asano R., Suzuki M., Tsumoto K., Sakurai N., Katayose Y., Kodama H., Yoshida
H., Ebara S., Saeki H., Imai K., Matsuno S., Kumagai I.
118) T-cell immunotherapy for human MK-1-expressing tumors using a fusion protein of the superantigen SEA and
anti-MK-1 scFv antibody. [Anticancer Res., 22 (2A), (2002), 769-776]
Ueno A., Arakawa F., Abe H., Matsumoto H., Kudo T., Asano R., Tsumoto K., Kumagai I., Kuroki M.
119) Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay: application of
selected VH to histochemistry. [J. Immunol. Meth., 261 (1-2), (2002), 65-72]
Nakamura M., Tsumoto K., Ishimura K., Kumagai I.
120) Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera. [J. Biosci. Bioeng., 93 (4), (2002), 399-404]
Kawahara M., Ueda H., Tsumoto K., Kumagai I., Mahoney W., Nagamune T.
121) Circular and magnetic circular dichroism studies of bacteriochlorophyll c aggregates: T-shaped and antiparallel
dimers. [J. Phys. Chem. B, 106 (15), (2002), 3987-3995]
Umetsu M., Seki R., Wang Z., Kumagai I., Nozawa T.
122) Detection of biotinylated proteins in polyacrylamide gels using an avidin-ﬂuorescein conjugate.
Biochem., 304 (2), (2002), 231-235]
[Anal.
Nakamura M., Tsumoto K., Ishimura K., Kumagai I.
123) The eﬀect of an agglutogen on virus infection: biotinylated ﬁlamentous phages and avidin as a model. [FEBS
Lett., 520 (1-3), (2002), 77-80]
Nakamura M., Tsumoto K., Ishimura K., Kumagai I.
124) Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of
target antigens. [Biochem. Biophys. Res. Commun., 295 (1), (2002), 31-36]
Watanabe H., Tsumoto K., Asano R., Nishimiya Y., Kumagai I.
125) Inhibition of hepatitis C virus NS3 protease by peptides derived from complementarity-determining regions
(CDRs) of the monoclonal antibody 8D4: tolerance of a CDR peptide to conformational changes of a target.
[FEBS Lett., 525 (1-3), (2002), 77-82]
Tsumoto K., Misawa S., Ohba Y., Ueno T., Hayashi H., Kasai N., Watanabe H., Asano R., Kumagai I.
126) Antitumor activity of Interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in
Escherichia coli. [FEBS Letters, 528 (1-3), (2002), 70-76]
Asano R., Kudo T., Makabe K., Tsumoto K., Kumagai I
127) Chiral recognition in the binding of helicenediamine to double strand DNA: interactions between low molecular
weight helical compounds and a helical polymer. [Bioorg. Med. Chem., 10 (10), (2002), 3213-3218]
Honzawa S., Okubo H., Anzai S., Yamaguchi M., Tsumoto K., Kumagai I.
128) Eﬃcient construction of a diabody using a refolding system: anti-carcinoembryonic antigen recombinant
antibody fragment. [J. Biochem., 132 (6), (2002), 903-909]
Asano R., Kudo T., Nishimura Y., Makabe K., Hayashi H., Suzuki M., Tsumoto K., Kumagai I.
129) A morphologic study of ﬁlamentous phage infection of Escherichia coli using biotinylated phages. [FEBS Lett.,
536 (1-3), (2003), 167-172]
Nakamura M., Tsumoto K., Kumagai I., Ishimura K.
130) The role of hydrogen bonding via interfacial water molecules in antigen-antibody complexation. The HyHEL10-HEL interaction. [ J. Biol. Chem., 278 (7), (2003), 5410-5418]
Yokota A., Tsumoto K., Shiroishi M., Kondo H., Kumagai I.
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131) Practical considerations in refolding proteins from inclusion bodies. [Protein Expr. Purif., 28 (1), (2003),
1-8]
Tsumoto K., Ejima D., Kumagai I., Arakawa T.
132) How additives inﬂuence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system.
Spectroscopic evidence for highly eﬃcient refolding of a single-chain Fv fragment. [J. Biol. Chem., 278
(11), (2003), 8979-8987]
Umetsu M., Tsumoto K., Hara M., Ashish K., Goda S., Adschiri T., Kumagai I.
133) Bypassing antibiotic selection: positive screening of genetically modiﬁed cells with an antigen-dependent
proliferation switch. [Nucleic Acids Res., 31 (7), (2003), e32]
Kawahara M., Ueda H., Morita S., Tsumoto K., Kumagai I ., Nagamune T.
134) Immobilized oxidoreductase as an additive for refolding inclusion bodies: application to antibody fragments.
[Protein Eng., 16 (7), (2003), 535-541]
Tsumoto K., Umetsu M., Yamada H., Ito T., Misawa S., Kumagai I.
135) Structural consequences of target epitope-directed functional alteration of an antibody. The case of anti-hen
lysozyme antibody, HyHEL-10. [J. Biol. Chem., 278 (27), (2003), 24929-24936]
Kumagai I., Nishimiya Y., Kondo H., Tsumoto K.
136) Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding
and bind preferentially to HLA-G. [Proc. Natl. Acad. Sci.USA, 100 (15), (2003), 8856-8861]
Shiroishi M., Tsumoto K., Amano K., Shirakihara Y., Colonna M., Braud V.M., Allan D.S., Makadzange
A., Rowland-Jones S., Willcox B., Jones E.Y., van der Merwe P.A., Kumagai I., Maenaka K.
137) An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation. [J. Immunol. Meth., 279 (1-2), (2003), 209-218]
Ueda H., Yokozeki T., Arai R., Tsumoto K., Kumagai I., Nagamune T.
138) On-column refolding and characterization of soluble human interleukin-15 receptor α-chain produced in
Escherichia coli. [Protein Expr. Purif., 31 (1), (2003), 64-71]
Matsumoto M., Misawa S., Tsumoto K., Kumagai I., Hayashi H., Kobayashi Y.
139) Stable expression of the motor protein prestin in chinese hamster ovary cells. [JSME Int. J., Ser. C, 46 (4),
(2003), 1266-1274]
Iida K., Konno K., Oshima T., Tsumoto K., Ikeda K., Kumagai I., Kobayashi T., Wada H.
140) Solubilization of active green ﬂuorescent protein from insoluble particles by guanidine and arginine. [Biochem.
Biophys. Res. Commun., 312 (4), (2003), 1383-1386]
Tsumoto K., Umetsu M., Kumagai I., Ejima D., Arakawa T.
141) AMEGA: antigen-mediated genetically modiﬁed cell ampliﬁcation. [J. Immunol. Meth., 284 (1-2), (2004),
187-194]
Kawahara M., Ueda H., Tsumoto K., Kumagai I., Nagamune T.
142) Structural implications for heavy metal-induced reversible assembly and aggregation of a protein: the case of
Pyrococcus horikoshii CutA. [FEBS Lett., 556 (1-3), (2004), 167-174]
Tanaka Y., Tsumoto K., Nakanishi T., Yasutake Y., Sakai N., Yao M., Tanaka I., Kumagai I.
143) Structural characteristics and refolding of in vivo aggregated hyperthermophilic archaeon proteins. [FEBS
Lett., 557 (1-3), (2004), 49-56]
Umetsu M., Tsumoto K., Ashish K., Nitta S., Tanaka Y.
144) Fucose depletion from human IgG1 oligosaccharide enhances binding enthalpy and association rate between
IgG1 and Fc γ RIIIa. [J. Mol. Biol., 336 (5), (2004), 1239-1249]
Okazaki A., Shoji-Hosaka E., Nakamura K., Wakitani M., Uchida K., Kakita S., Tsumoto K., Kumagai
I., Shitara K.
生体機能化学講座（タンパク質工学分野）
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145) Phage-display selection of antibodies to the left end of CTX3C using synthetic fragments. [J. Immunol. Meth.,
289 (1-2), (2004), 137-146]
Nagumo Y., Oguri H., Tsumoto K., Shindo Y., Hirama M., Tsumuraya T., Fujii I., Tomioka Y., Mizugaki
M., Kumagai I.
146) A highly eﬀective and stable bispeciﬁc diabody for cancer immunotherapy: cure of xenografted tumors by
bispeciﬁc diabody and T-LAK cells. [Cancer Immunol. Immunother., 53 (6), (2004), 497-509]
Hayashi H., Asano R., Tsumoto K., Katayose Y., Suzuki M., Unno M., Kodama H., Takemura S., Yoshida
H., Makabe K., Imai K., Matsuno S., Kumagai I., Kudo T.
147) How oligomerization contributes to the thermostability of an archaeon protein. Protein L-isoaspartyl-Omethyltransferase from Sulfolobus tokodaii. [J. Biol. Chem., 279 (31), (2004), 32957-32967]
Tanaka Y., Tsumoto K., Yasutake Y., Umetsu M., Yao M., Fukada H., Tanaka I., Kumagai I.
148) Reversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse
agonism in cytokine receptor superfamily. [Biochem. Pharmacol., 68 (3), (2004), 539-548]
Kawahara M., Ishii S., Tsumoto K., Kumagai I., Ueda H., Nagamune T.
149) Role of arginine in protein refolding, solubilization, and puriﬁcation. [Biotechnol. Prog., 20 (5), (2004),
1301-1308]
Tsumoto K., Umetsu M., Kumagai I., Ejima D., Philo J.S., Arakawa T.
150) Antigen-mediated genetically modiﬁed cell ampliﬁcation (AMEGA) with single vector transduction. [J. Chem.
Eng. Jpn., 37 (10), (2004), 1259-1264]
Kaneko E., Kawahara M., Tsumoto K., Kumagai I., Ueda H., Nagamune T.
151) Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus
horikoshii in 3 M guanidine hydrochloride. Biochem. [Biochem. Biophys. Res. Commun., 323 (1), (2004),
185-191]
Tanaka Y., Tsumoto K., Umetsu M., Nakanishi T., Yasutake Y., Sakai N., Yao M., Tanaka I., Arakawa
T., Kumagai I.
152) Relationship between ﬂuorescence intensity of GFP and the expression level of prestin in a prestin-expressing
Chinese hamster ovary cell line. [JSME Int. J., Ser. C, 47 (4), (2004), 970-976]
Iida K., Nagaoka T., Tsumoto K., Ikeda K., Kumagai I., Kobayashi T., Wada H.
153) to insoluble aggregates on the refolding of a single-chain Fv antibody: morphological changes of aggregated
protein on refolding. [Chem. Lett. (Jpn.), 33 (12), (2004), 1600-1601]
Umetsu M., Ashish K., Tsumoto K., Kumagai I.
154) Tumor-directed lymphocyte-activating cytokines: refolding-based preparation of recombinant human
interleukin-12 and an antibody variable domain-fused protein by additive-introduced stepwise dialysis.
[Biochem. Biophys. Res. Commun., 328 (1), (2005), 98-105]
Makabe K., Asano R., Ito T., Tsumoto K., Kudo T., Kumagai I.
155) Nondenaturing solubilization of beta2 microglobulin from inclusion bodies by L-arginine. [Biochem. Biophys.
Res. Commun., 328 (1), (2005), 189-197]
Umetsu M., Tsumoto K., Nitta S., Adschiri T., Ejima D., Arakawa T., Kumagai I.
156) Herpes simplex virus targeting to the EGF receptor by a gD-speciﬁc soluble bridging molecule. [ Mol. Ther.,
11 (4), (2005), 617-626]
Nakano K., Asano R., Tsumoto K., Kwon H., Goins W.F., Kumagai I.
157) Construction of an expression system for the motor protein prestin in Chinese hamster ovary cells. [Hear Res.,
205 (1-2), (2005), 262-270]
Iida K., Tsumoto K., Ikeda K., Kumagai I., Kobayashi T.
158) Eﬀects of mutation in the conserved GTSRH sequence of the motor protein prestin on its characteristics.
[JSME Int. J., Ser. C, 48 (4), (2005), 403-410]
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Kumano S., Iida K., Murakoshi M., Naito N., Tsumoto K., Ikeda K., Kumagai I., Kobayashi T., Wada H.
159) Crystal structure of the hypothetical protein ST2072 from Sulfolobus tokodaii. [Proteins, 61 (4), (2005),
1127-1131]
Tanaka Y., Tsumoto K., Tanabe E., Yasutake Y., Sakai N., Yao M., Tanaka I., Kumagai I.
160) Bioassisted room-temperature immobilization and mineralization of zinc oxide -The structural ordering of ZnO
nanoparticles into a ﬂower-type morphorogy. [Adv. Mater., 17 (21), (2005), 2571-2575]
Umetsu M., Mizuta M., Tsumoto K., Ohara S., Takami S., Watanabe H., Kumagai I., Adschiri T.
161) Entropically driven MHC class I recognition by human inhibitory receptor leukocyte Ig-like receptor B1
(LILRB1/ILT2/CD85j). [J. Mol. Biol., 355 (2), (2006), 237-248]
Shiroishi M., Kuroki K., Tsumoto K., Yokota A., Sasaki T., Amano K., Shimojima T., Shirakihara Y.,
Rasubala L., van der Merwe P.A., Kumagai I., Kohda D., Maenaka K.
162) Eﬃcient leukocyte Ig-like receptor signaling and crystal structure of disulﬁde-linked HLA-G dimer. [J. Biol.
Chem., 281 (15), (2006), 10439-10447]
Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I.
163) Humanization of the bispeciﬁc epidermal growth factor receptor x CD3 diabody and its eﬃcacy as a potential
clinical reagent. [Clin. Cancer Res., 12 (13), (2006), 4036-4042]
Asano R., Sone Y., Makabe K., Tsumoto K., Hayashi H., Katayose Y., Unno M., Kudo T., Kumagai I.
164) Imaging by atomic force microscopy of the plasma membrane of prestin-transfected Chinese hamster ovary
cells. [J. Assoc. Res. Otolaryngol., 7 (3), (2006), 267-278]
Murakoshi M., Gomi T., Iida K., Kumano S., Tsumoto K., Kumagai I., Ikeda K., Kobayashi T., Wada H.
165) Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2
(LILRB2/LIR2/ILT4/CD85d). [Proc. Natl. Acad. Sci. USA., 103 (44), (2006), 16412-16417]
Shiroishi M., Kuroki K., Rasubala L., Tsumoto K., Kumagai I., Kurimoto E., Kato K., Kohda D., Maenaka
K.
166) Growth control of hybridoma cells with an artiﬁcially induced EpoR-gp130 heterodimer. [Cytotechnology, 52
(13), (2006), 171-179]
Kawahara M., Ogo Y., Tsumoto K., Kumagai I., Ueda H., Nagamune T
167) Structural consequences of mutations in interfacial Tyr residues of a protein antigen-antibody complex. The
case of HyHEL-10-HEL. [J. Biol. Chem., 282 (9), (2007), 6783-6791]
Shiroishi M., Tsumoto K., Tanaka Y., Yokota A., Nakanishi T., Kondo H., Kumagai I.
168) Mimicry of erythropoietin and interleukin-6 signalling by an antibody/cytokine receptor chimera in murine
myeloid 32D cells. [J. Biochem. (Tokyo), 141 (4), (2007), 563-571]
Kawahara M., Ueda H., Tsumoto K., Kumagai I., Nagamune T.
169) Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical
microscopy (SECM). [Biotechnol. Bioeng., 96 (5), (2007), 1008-1013]
Nagamine K., Onodera S., Kurihara A., Yasukawa T., Shiku H., Asano R., Kumagai I.
170) A Human antibody fragment with high aﬃnity for biodegradable polymer ﬁlm. [Bioconjug. Chem., 18 (3),
(2007), 645-651]
Watanabe H., Tsumoto K., Taguchi S., Yamashita K., Doi Y., Nishimiya Y., Kondo H., Umetsu M.,
Kumagai I.
171) Molecular properties of two proteins homologous to PduO-type ATPcob(I)alamin adenosyltransferase from
Sulfolobus tokodaii. [Proteins, 68 (2), (2007), 446-457]
Tanaka Y., Sasaki T., Kumagai I., Yasutake Y., Yao M., Tanaka I., Tsumoto K.
172) Highly eﬀective recombinant format of a humanized IgG-like bispeciﬁc antibody for cancer immunotherapy
with retargeting of lymphocytes to tumor cells. [J. Biol. Chem., 282 (28), (2007), 27659-27665]
生体機能化学講座（タンパク質工学分野）
0 – 15
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Asano R., Watanabe Y., Kawaguchi H., Fukazawa H., Nakanishi T., Umetsu M., Hayashi H., Katayose
Y., Unno M., Kudo T., Kumagai I.
173) Human CD4+ central and eﬀector memory T cells produce IL-21: eﬀect on cytokine-driven proliferation of
CD4+ T cell subsets. [Int. Immunol., 19 (10), (2007), 1191-1199]
Onoda T., Rahman M., Nara H., Araki A., Makabe K., Tsumoto K., Kumagai I. , Kudo T., Ishii N.,
Tanaka N., Sugamura K., Hayasaka K., Asao H.
174) High IL-21 receptor expression and apoptosis induction by IL-21 in follicular lymphoma. [Cancer Lett., 256
(2), (2007), 196-206]
Akamatsu N., Yamada Y., Hasegawa H., Makabe K., Asano R., Kumagai I., Murata K., Imaizumi Y.,
Tsukasaki K„ Tsuruda K., Sugahara K., Atogami S., Yanagihara K., Kamihira S.
175) Thermodynamic consequences of mutations in Vernier zone residues of a humanized anti-human epidermal
growth factor receptor (EGFR) murine antibody, 528. [J. Biol. Chem., 283 (2), (2008), 1156-1166]
Makabe K., Nakanishi T., Tsumoto K., Tanaka Y., Kondo H., Umetsu M., Sone Y., Asano R., Kumagai
I.
176) Grafting of material-binding function into antibodies Functionalization by peptide grafting.
Biophys. Res. Commun., 365 (4), (2008), 751-757]
[Biochem.
Hattori T., Umetsu M., Nakanishi T., Tsumoto K., Ohara S., Abe H., Naito M., Asano R., Adschiri T.,
Kumagai I
177) Critical Contribution of VH-VL Interaction to Reshaping of an Antibody: The case of humanization of antilysozyme antibody, HyHEL-10. [Protein Sci., 17 (2), (2008), 261-270]
Nakanishi T., Tsumoto K., Yokota A., Kondo H., Kumagai I.
178) Critical contribution of aromatic rings to speciﬁc recognition of polyether rings. The case of ciguatoxin CTX3CABC and its speciﬁc antibody 1C49. [J. Biol. Chem., 283 (18), (2008), 12259-12266]
Tsumoto K., Yokota A., Tanaka Y., Ui M., Tsumuraya T., Fujii I., Kumagai I., Nagumo Y., Oguri H.,
Inoue M., Hirama M.
179) Selective expansion of genetically modiﬁed T cells using an antibody/interleukin-2 receptor chimera. [J.
Immunol. Meth., 337 (1), (2008), 16-23]
Sogo T., Kawahara M., Tsumoto K., Kumagai I., Ueda H., Nagamune T.
180) Human Anti-gold Antibodies: BIOFUNCTIONALIZATION OF GOLD NANOPARTICLES AND SURFACES
WITH ANTI-GOLD ANTIBODIES. [J. Biol. Chem., 31 (8), (2008), 752-761]
Watanabe H., Nakanishi T., Umetsu M., Kumagai I.
181) Diabody-based recombinant formats of humanized IgG-like bispeciﬁc antibody with eﬀective retargeting of
lymphocytes to tumor cells. [J. Immunother., 31 (8), (2008), 752-761]
Asano R., Kawaguchi H., Watanabe Y., Nakanishi T., Umetsu M., Hayashi H., Katayose Y., Unno M.,
Kudo T., Kumagai I.
182) Preferential heterodimerization of a bispeciﬁc diabody based on a humanized anti-EGFR antibody 528.
[Protein Eng. Des. Sel., 21 (10), (2008), 597-603]
Asano R., Sone Y., Ikoma K., Hayashi H., Nakanishi T., Umetsu M., Katayose Y., Unno M., Kudo T.,
Kumagai I.
183) Nanoparticles with aﬃnity for biopolymer:Bioassisted room-temperature selective multistacking of inorganic
particles on biopolymer ﬁlm. [J. Mater. Res., 23 (12), (2008), 3241-3246]
Umetsu M., Hattori T., Kikuchi K., Muto I., Nakanishi T., Watanabe H., Kumagai I.
184) Electrochemical detection of epidermal growth factor receptors on a single living cell surface by scanning
electrochemical microscopy. [Anal Chem., 146 (5), (2009), 651-657]
Takahashi Y., Miyamoto T., Shiku H., Asano R., Yasukawa T., Kumagai I., Matsue T.
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185) Importance of the hydrogen bonding network including Asp52 for catalysis, as revealed by Asn59 mutant hen
egg-white lysozymes. [J. Biochem., 146 (5), (2009), 651-657]
Ose T., Kuroki K., Matsushima M., Maenaka K., Kumagai I.
186) Application of the Fc fusion format to generate tag-free bi-speciﬁc diabodies. [FEBS J., 277 (1), (2010),
477-487]
Asano R., Ikoma K., Kawaguchi H., Ishiyama Y., Nakanishi T., Umetsu M., Hayashi H., Katayose Y.,
Unno M., Kudo T., Kumagai I.
187) Glycosylation pattern of humanized IgG-like bispeciﬁc antibody produced by recombinant CHO cells. [Appl.
Microbiol. Biotechnol., 85 (3), (2010), 535-542]
Kim W.D., Tokunaga M., Ozaki H., Ishibashi T., Honda K., Kajiura H., Fujiyama K., Asano R., Kumagai
I., Omasa T., Ohtake H.
188) Direct and selective immobilization of proteins by means of an inorganic material-binding peptide: discussion
on functionalization in the elongation to material-binding peptide. [J. Phys. Chem. B, 114 (1), (2010),
480-486]
Yokoo N., Togashi T., Umetsu M., Tsumoto K., Hattori T., Nakanishi T., Ohara S., Takami S., Naka T.,
Abe H., Kumagai I., Adschiri T.
189) High aﬃnity anti-inorganic material antibody generation by integrating graft and evolution technologies:
potential of antibodies as biointerface molecules. [J. Biol. Chem., 285 (10), (2010), 7784-7793]
Hattori T., Umetsu M., Nakanishi T., Togashi T., Yokoo N., Abe H., Ohara S., Adschiri T., Kumagai I.
190) Contribution of asparagine residues to the stabilization of a proteinaceous antigen-antibody complex, HyHEL10-hen egg white lysozyme. [J. Biol. Chem., 285 (10), (2010), 7784-7793]
Yokota A., Tsumoto K., Shiroishi M., Nakanishi T., Kondo H., Kumagai I.
191) Protein-protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary
structural information. [FEBS J., 277 (9), (2010), 2006-2014]
Umetsu M., Nakanishi T., Asano R., Hattori T., Kumagai I.
192) Target cell-restricted apoptosis induction by 528scFv-TRAIL fusion protein speciﬁc for human EGFR and
expressed in Escherichia coli. [Int. J. Oncol., 36 (5), (2010), 1229-1234]
Badran A., Asano R., Nakayama M., Watanabe Y., Nakanishi T., Umetsu M., Hayashi H., Katayose Y.,
Unno M., Kumagai I.
193) Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates
produced in bacteria - Application to a single-chain Fv fragment -.. [J. Biosci. Bioeng., 109 (5), (2010),
447-452]
Kumagai I., Asano R., Nakanishi T., Hashikami K., Tanaka S., Badran A., Umetsu M.
194) Direct immobilization of gold-binding antibody fragments for immunosensor applications. [Anal. Chem., 82
(10), (2010), 4229-4235]
Ibii T., Kaieda M., Hatakeyama S., Shiotsuka H., Watanabe H., Umetsu M., Kumagai I., Imamura T.
195) Salicylate-induced translocation of prestin having mutation in the GTSRH sequence to the plasma membrane.
[FEBS Lett., 584 (11), (2010), 2327-2332]
Kumano S., Iida K., Ishihara K., Murakoshi M., Tsumoto K., Ikeda K., Kumagai I., Kobayashi T., Wada
H.
196) Highly enhanced cytotoxicity of a dimeric bispeciﬁc diabody, the hEx3 tetrabody. [J. Biol. Chem., 285 (27),
(2010), 20844-20849]
Asano R., Ikoma K.,Sone Y., Kawaguchi H., Taki S., Hayashi H., Nakanishi T., Umetsu M., Katayose Y.,
Unno M., Kudo T., Kumagai I.
197) Novel anti-Tn single-chain Fv-Fc fusion proteins derived from immunized phage library and antibody Fc domain.
[Anticancer Res., 30 (9), (2010), 3397-3405]
Kubota T., Matsushita T., Niwa R., Kumagai I., Nakamura K.
生体機能化学講座（タンパク質工学分野）
0 – 17
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198) Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells. [J.
Immunol. Methods., 361 (1-2), (2010), 57-63]
Sasajima Y., Iwasaki R., Tsumoto K., Kumagai I., Ihara M., Ueda H.
199) A double mutation in glycoprotein gB compensates for ineﬀective gD-dependent initiation of herpes simplex
virus type 1 infection. [J. Virol., 84 (23), (2010), 12200-12209]
Uchida H., Chan J., Goins W.F., Grandi P., Kumagai I., Cohen J.B., Glorioso J.C.
200) Cytotoxic Enhancement of a Bispeciﬁc Diabody by Format Conversion to Tandem Single-chain Variable
Fragment (taFv) THE CASE OF THE hEx3 DIABODY. [J. Biol. Chem., 286 (3), (2011), 1812-1818]
Asano R., Ikoma K., Shimomura I., Taki S., Nakanishi T., Umetsu M., Kumagai I.
201) Material-binding peptide application-ZnO crystal structure control by means of a ZnO-binding peptide. [J.
Biosci. Bioeng., 111 (2), (2011), 140-145]
Togashi T., Yokoo N., Umetsu M., Ohara S., Naka T., Takami S., Abe H., Kumagai I., Adschiri T.
202) Enhancement of Cellulolytic Enzyme Activity by Clustering Cellulose Binding Domains on Nanoscaﬀolds.
[Small, 7 (5), (2011), 656-664]
Kim D.M., Umetsu M., Takai K., Matsuyama T., Ishida N., Takahashi H., Asano R., Kumagai I.
203) Mechanism of HSV infection through soluble adapter-mediated virus bridging to the EGF receptor. [Virology,
413 (1), (2011), 12-18]
Nakano K., Kobayashi M., Nakamura K., Nakanishi T., Asano R., Kumagai I., Tahara H., Kuwano M.,
Cohen J.B., Glorioso J.C.
204) Bioassisted capture and release of nanoparticles on nanolithographed ZnO ﬁlms. [Nanotechnology, 22 (27),
(2011), 275302]
Shimada Y., Suzuki M., Sugiyama M., Kumagai I., Umetsu M.
205) Zinc oxide-coated plasmonic chip modiﬁed with a bispeciﬁc antibody for sensitive detection of a ﬂuorescent
labeled-antigen. [Anal. Chem., 83 (15), (2011), 5944-5948]
Tawa K., Umetsu M., Hattori T., Kumagai I.
206) Biomimetic engineering of modular bispeciﬁc antibodies for biomolecule immobilization. [Langmuir, 27 (16),
(2011), 9656-9661]
Watanabe H., Kanazaki K., Nakanishi T., Shiotsuka H., Hatakeyama S., Kaieda M., Imamura T., Umetsu
M., Kumagai I.
207) Electrochemical detection of receptor-mediated endocytosis by scanning electrochemical microscopy. [Phys.
Chem. Chem. Phys., 13 (37), (2011), 16569-16573]
Takahashi Y., Miyamoto T., Shiku H., Ino K., Yasukawa T., Asano R., Kumagai I., Matsue T.
208) In vitro and in vivo antitumor eﬀects of recombinant bispeciﬁc antibodies based on humanized anti-EGFR
antibody. [Oncol. Rep., 26 (4), (2011), 949-955]
Watanabe Y., Asano R., Arai K., Shimomura I., Ogata H., Kawaguchi H., Hayashi H., Ohtsuka H.,
Yoshida H., Katayose Y., Egawa S., Nakanishi T., Umetsu M., Yasui H., Ishida T., Imai K., Kudo T.,
Unno M., Kumagai I.
209) Construction and humanization of a functional bispeciﬁc EGFR × CD16 diabody using a refolding system.
[FEBS J., 279 (2), (2012), 223-233]
Asano R., Nakayama M., Kawaguchi H., Kubota T., Nakanishi T., Umetsu M., Hayashi H., Katayose Y.,
Unno M., Kudo T., Kumagai I.
210) Ionic liquid/water interfacial localization of a green ﬂuorescent protein fused to a tryptophan-rich peptide. [J.
Biosci. Bioeng., 113 (2), (2012), 160-165]
Matsuyama T., Domyoung K., Umetsu M., Ikawa T., Yamanishi M., Ishida N., Kumagai I., Takahashi H.
211) A nanocluster design for the construction of artiﬁcial cellulosomes. [Catal. Sci. Technol., 2 (3), (2012),
499-503]
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Kim D.M., Nakazawa H., Umetsu M., Takai K., Matsuyama T., Ishida N., Ikeuchi A., Takahashi H.,
Asano R., Kumagai I.
212) Enhancement of sialylation on humanized IgG-like bispeciﬁc antibody by overexpression of α 2,6sialyltransferase derived from Chinese hamster ovary cells. [Appl. Microbiol. Biotechnol., 94 (1), (2012),
69-80]
Onitsuka M., Kim W.D., Ozaki H., Kawaguchi A., Honda K., Kajiura H., Fujiyama K., Asano R., Kumagai
I., Ohtake H., Omasa T.
213) A High-Aﬃnity Gold-Binding Camel Antibody: Antibody Engineering for One-Pot Functionalization of Gold
Nanoparticles as Biointerface Molecules. [Bioconjugate Chem., 23 (9), (2012), 1934-1944]
Hattori T., Umetsu M., Nakanishi T., Sawai S., Kikuchi S., Asano R., Kumagai I.
214) Quantum Dot Motion on Microtubules. [Chem. Lett., 41 (10 SI), (2012), 1215-1217]
Sikora A., Oliveira D., Kim K., Liao A.L., Umetsu M., Kumagai I., Adschiri T., Hwang W., Teizer W.
215) The assembly of kinesin-based nanotransport systems. [J. Appl. Phys., 112 (12), (2012), 124703]
Oliveira D., Kim D.M., Umetsu M., Kumagai I., Adschiri T., Teizer W.
216) Development of an aﬃnity-matured humanized anti-epidermal growth factor receptor antibody for cancer
immunotherapy. [Protein Eng. Des. Sel., 26 (2), (2013), 113-122]
Nakanishi T., Maru T., Tahara K., Sanada H., Umetsu M., Asano R., Kumagai I.
総説・解説記事
1) Methylation of guanosine 2’-OH in the D-loop of tRNA from an extreme thermophile, thermusthermophilus.
[Metabolism and Enzymology of Nucleic Acids, 4, (1982), 361-371]
Kumagai I., Watanabe K., Oshima T.
2) Nucleotide Sequence and Secondary Structure Analysis of Spinach Chloroplast 4.5S RNA. [Hoppe Seylers Z.
Physiol. Chem., 363, (1982), 880]
Kumagai I., Pielar T., Subramanian A.R., Erdmann V.A.
3) Chemical modiﬁcation of 5S and 4.5S rRNAs in situ in spinach chloroplast ribosomes. [Nucleic Acids Research
Symposium Series, 12, (1983), 159-160]
Kumagai I., Bartsch M., Subramanian A.R., Erdmann V.A.
4) Chemical synthesis of capped RNA fragments and their ability to complex with eukaryotic ribosomes. [Nucleic
Acids Research Symposium Series, 14, (1985), 223-236]
Kohno K., Nishiyama S., Kamimura T., Sekine M., Hata T., Kumagai I., Miura K.
5) Eﬀects of modiﬁcation of 4-thiouridine in E.coli tRNAfM et on methylation reaction with a thermostableGmmethylase. [Nucleic Acids Research Symposium Series, 17, (1986), 175-178]
Hori H., Watanabe K., Saneyoshi M., Kumagai I., Hirao I., Miura K.
6) 分子レベル-タンパク質工学. [BIO INDUSTRY, 3 (7), (1986), 538-545]
熊谷泉
7) 遺伝子工学による放線菌プロテアーゼインヒビターの人工的改変. [Journal of the Faculty of Engineering The
University of Tokyo Series A, 25, (1987), 5-8]
三浦謹一郎, 熊谷泉, 小畑充生, 小島修一, 田口精一, 古久保進
8) Construction of a plasmid for high-level expression of goat a-lactalbumin and its derivative in E.coli. [Nucleic
Acids Research Symposium Series, 19, (1988), 179-180]
Kumagai I., Inaizumi M., Tamaki E., Misawa S., Hibino T., Miura K.
9) 酵素などの機能改善をめざしてのタンパク質の改造. [化学増刊 113 プロテインエンジニアリング新しいタ
ンパク質のデザインと合成, (1988), 139-151]
生体機能化学講座（タンパク質工学分野）
0 – 19
熊谷泉
熊谷泉, 三浦謹一郎
10) タンパク質工学とタンパク質の化学修飾. [化学と工業, 41 (12), (1988), 1102-1105]
熊谷泉
11) 放線菌サブチリシンインヒビターの蛋白質工学. [蛋白質核酸酵素„ 34 (8), (1989), 939-948]
小島修一, 熊谷泉, 三浦謹一郎
12) アミノ酸変換による c 型リゾチームの機能解析. [蛋白質核酸酵素 · 臨時増刊遺伝子操作, 35, (1990),
2632-2642]
熊谷泉, 砂田太, 武田茂樹
13) プロテアーゼインヒビター SSI. [現代化学 · 増刊 19 プロテインエンジニアリング, 35, (1990), 2632-2642]
小島修一, 熊谷泉, 三浦謹一郎
14) 大気圧化学イオン化 (APCI) LC/MS によるアミノ酸 PTH 誘導体の分析. [生産研究, 43, (1990), 359-362]
内山秀文, 三浦謹一郎, 熊谷泉, 高井信冶
15) 抗体の人工設計. [化学, 46, (1991), 500]
熊谷泉
16) 酵素機能実現をめざしたα-ラクトアルブミンの蛋白質工学. [蛋白質核酸酵素 · 臨時増刊蛋白質工学の進
展, 37, (1992), 371-380]
熊谷泉, 武田茂樹
17) Relationship between the 5’-leader sequence of mRNAs and the translation eﬃciency in a eukaryotic in vitro
system. [Nucleic Acids Symposium Series, 27, (1992), 159-160]
Wakiyama M., Hirao I., Kumagai I., Watanabe K., Miura K.
18) Puriﬁcation and characterization of tRNA(adenosine-1-)-methyl transferase from Thermus thermophilus HB27.
[Nucleic Acids Symposium Series, 27, (1992), 141-142]
Yamazaki N., Hori H., Ozawa K., Nakanishi S., Ueda T., Kumagai I., Watanabe K., Nishikawa K.
19) たんぱく質の分子設計をめざして. [ペテロテック, 15 (2), (1992), 131-137]
熊谷泉
20) 繊維状ファージ表面における機能性蛋白質ライブラリーの構築. [バイオサイエンスとインダストリー, 51,
(1993), 991-992]
津本浩平, 熊谷泉
21) タンパク質の機能構造とエキソン-エキソン領域の交換による機能変換-. [細胞工学, 12, (1993), 43-48]
熊谷泉
22) 放線菌ズブチリシンインヒビター. [現代化学 · 増刊プロテアーゼと生体機能、分子から病態まで, 22, (1993),
105-111]
熊谷泉
23) Interaction between a recombinant Fv fragment and its antigen protein: Eﬀect of structural conversions of the
antigenic epitope. [Protein Eng., 7, (1993), 1010]
Tsumoto K., Ueda Y., Maenaka K., Ogawawara K., Yutani K., Watanabe K., Kumagai I.
24) タンパク質工学に基づく c 型リゾチームの機能解析. [生物物理, 33 (4), (1993), 219-224]
前仲勝実, 熊谷泉
25) 回折データ収集法の進展-より速く, より美しく- a) 実験室系. [生物物理, 33 (6), (1993), 311-313]
松島正明, 熊谷泉, 前仲勝実
26) 繊維状ファージコートタンパク質への異種タンパク質の提示-ファージ抗体を中心として-. [生化学, 65 (12),
(1993), 1508-1512]
0 – 20
バイオ工学専攻
熊谷泉
熊谷泉
27) たん白質工学の進展と課題. [電子情報通信学会誌, 77 (11), (1994), 1154-1160]
熊谷泉
28) 楽しい化学の実験室-遺伝子の実体をみる-. [現代化学, 8, (1995), 51-54]
熊谷泉
29) A single-chain Fv fragment recognizing a protease inhibitor. [Protein Eng., 9, (1995), 960-961]
Hayashi R., Tsumoto K., Furuta M., Watanabe K., Uchikawa M., Juji T., Kojima S., Miura K., Kumagai
I.
30) Development of the stable phagemid pIII display system of proteins on the surface of the ﬁlamentous phage.
[Protein Eng., 9, (1995), 965]
Maenaka K., Tsumoto K., Ueda Y., Furuta M., Watanabe K., Kumagai I.
31) 抗体の分子内相互作用を利用した新しい免疫測定法. [ケミカルエンジニヤリング, 41, (1996), 878-882]
上田宏, 津本浩平, 久保田一石, 長棟輝行, 熊谷泉
32) Development of a novel phage display system and its application to conversion of molecular recognition activities
of proteins. [Protein Eng., 9, (1996), 812]
Kumagai I., Tsumoto K., Maenaka K.
33) Functional conversion of antibody variable region by phage display technology. [Protein Eng., 10, (1996),
1001-1002]
Tsumoto K., Nishimiya Y., Kumagai I.
34) 触媒機構に基づく酵素分子の進化工学. [化学, 51 (6), (1996), 398-399]
前仲勝実, 熊谷泉
35) Essentially minimal sequence for substrate recognition by tRNA (guanosine-2’)- methyltransferase from
Thermus thermophilus HB27. [Nucleic Acids Symposium Series, 37, (1997), 189-190]
Hori H., Yamazaki N., Matsumoto T., Ueda T., Nishikawa K., Kumagai I., Watanabe K.
36) 抗体 VH-VL 相互作用と蛍光エネルギー移動を利用した均一系非競争的免疫測定法. [化学工学, 61 (3), (1997),
215-216]
上田宏, 津本浩平, 久保田一石, 長棟輝行, 熊谷泉
37) ファージ提示法による抗体工学. [生物物理, 37 (5), (1997), 219-222]
熊谷泉, 津本浩平, 前仲勝実
38) 生体外選択系による新機能抗体分子の創製. [蛋白質核酸酵素, 43 (2), (1998), 159-167]
熊谷泉, 津本浩平
39) 医薬品開発を目指したプロテインエンジニアリングの現状. [BIO INDUSTRY, 15 (4), (1998), 29-36]
三沢悟, 熊谷泉
40) 日本生化学会における当面の課題と将来の展開. [生化学, 70 (12), (1998), 1445-1449]
中澤淳, 石浦章一, 井上圭三, 江崎信芳, 大島泰郎, 熊谷泉, 清水孝雄
41) 新たな免疫測定法. [遺伝子医学, 3 (1), (1999), 170-175]
上田宏, 長棟輝行, 津本浩平, 熊谷泉
42) シリーズ「生理学者のための分子生物学技術講座」ファージライブラリーによる抗体産生. [日本生理学雑誌,
61 (1), (1999), 13-21]
熊谷泉, 津本浩平
43) 金属表面特異的ペプチドの選択-半導体と生命分子の接点. [化学, 55 (11), (2000), 58-59]
生体機能化学講座（タンパク質工学分野）
0 – 21
熊谷泉
熊谷泉, 津本浩平
44) 変異体を用いた抗原抗体相互作用の熱力学的、速度論的解析. [知識ベースシステム研究会, 49, (2000), 83-88]
津本浩平, 熊谷泉
45) 抗体ドメインの効率的巻き戻し法. [生物物理, 40 (4), (2000), 274-276]
津本浩平, 三沢悟, 熊谷泉
46) Review – Second generation bispeciﬁc antibodies : Applications in cancer immunotherapy. [Recent Res. Devel.
Cancer, 3, (2001), 235-255]
Kudo T., Suzuki M., Takemura S., Asano R., Tsumoto K., Katayose Y., Shinoda M., Sakurai N., Kodama
H., Ichiyama M., Yoshida H., Hayashi H., Saijyo S., Saito S., Takahashi J., Imai K., Kumagai I.
47) ファージディスプレイを用いた新規バイオ分子の選択と応用. [BIO INDUSTRY, 18 (6), (2001), 26-33]
津本浩平, 熊谷泉
48) 凝集蛋白質を再生する—効率的な凝集抑制手法の開発—. [蛋白質核酸酵素, 46 (9), (2001), 1238-1246]
津本浩平, 三沢悟, 熊谷泉
49) Antigen-mediated genetically modiﬁed cell ampliﬁcation. [Biological System Engineering chapter 11, ACS
SYMPOSIUM SERIES, 830, (2002), 140-151]
Kawahara M., Ueda H., Tsumoto K., Kumagai I., Mahoney W., Nagamune T.
50) Open sandwich selection -Selection of human antibody fragments using the mechanism of Fv fragment
stabilization in the presence of antigen. [Biological System Engineering chapter 23, ACS SYMPOSIUM
SERIES, 830, (2002), 285-295]
Tsumoto K., Watanabe H., Kumagai I.
51) 抗体医薬品開発における超高感度等温滴定型カロリメータの活用術. [BIO ベンチャー, 2, (2002), 116-117]
津本浩平, 熊谷泉
52) 凝集タンパク質の再生 ∼ 凝集抑制と巻き戻し ∼ . [BIO ベンチャー, 3 (5), (2003), 32-35]
津本浩平, 熊谷泉
53) クローズアップ実験法：アルギニンを用いた変性タンパク質のりフォールディング. [実験医学, 21, (2003),
1669-1673]
津本浩平, 熊谷泉, 荒川力
54) 新実験講座蛋白質リフォールディングの救世主, アルギニン. [蛋白質核酸酵素, 48 (16), (2003), 2310-2317]
荒川力, 勝山厳, 熊谷泉, 津本浩平
55) 凝集蛋白質の効率的再生と分光学的フォールディング研究の接点-抗体分子を中心として-. [生物物理, 44 (3),
(2004), 102-107]
梅津光央, 津本浩平, 熊谷泉
56) 概論：抗体療法の進歩-臨床応用への期待. [医歯薬出版株式会社医学のあゆみ, 211 (７), (2004), 723-727]
浅野竜太郎, 津本浩平, 熊谷泉
57) 巻頭言「産総研バイオ研究への期待」. [AIST Today, 5, (2005), 3]
熊谷泉
58) モノクローナル抗体のがん治療への応用. [がん分子標的治療, 4 (2), (2006), 98-104]
熊谷泉
59) 抗体医薬の現状と抗体工学の展望. [化学と生物, 44 (7), (2006), 459-465]
熊谷泉
60) 抗 EGF レセプター · 抗 CD3 二重特異性抗体の抗腫瘍作用. [臨床免疫, 45 (5), (2006), 497-502]
0 – 22
バイオ工学専攻
熊谷泉
熊谷泉, 林洋毅, 浅野竜太郎
61) 抗体ライブラリー技術の現状と今後の展望. [細胞工学, 26 (3), (2007), 254-257]
中西猛, 熊谷泉
62) リンパ球とがん細胞を認識するヒト型化二重特異性抗体. [Drug Delivery System, 23 (5), (2008), 518-525]
熊谷泉, 浅野竜太郎
63) 次世代抗体医薬の分子設計. [化学工業社ケミカルエンジニヤリング, 54 (1), (2009), 65-71]
熊谷泉, 浅野竜太郎
64) フォールドに基づくタンパク質工学. [The TRC News, 107, (2009), 1-12]
熊谷泉
65) ナノ材料も抗原？ポリマー · 金属表面構造を識別できる抗体分子. [化学同人月刊「化学」, 65, (2010), 66-67]
熊谷泉, 梅津光央
66) 材料ナノ表面を抗原とする抗体—ナノ世界の糊として—. [バイオサイエンスとインダストリー, 68 (1), (2010),
39-43]
梅津光央, 渡邊秀樹, 熊谷泉
67) ∼ 抗体医療研究の技術を工業材料開発へ ∼ 抗体分子の「ナノ糊（のり）
」としての利用. [MATERIAL STAGE,
10 (10), (2011), 6-9]
渡邊秀樹, 梅津光央, 熊谷泉
68) 高機能性次世代抗体医薬の分子設計. [ニューサイエンス社細胞, 43 (1), (2011), 24-28]
浅野竜太郎, 熊谷泉
69) 抗体医薬の大腸菌を用いた製造技術のあゆみ. [株式会社じほう PHARM TECH JAPAN, 27 (6), (2011),
67-73]
浅野竜太郎, 熊谷泉
70) 低分子抗体と抗体様スキャフォールドに基づく次世代抗体医薬の開発. [シーエムシー出版 BIO INDUSTRY,
28 (7), (2011), 22-27]
浅野竜太郎, 熊谷泉
71) タンパク質工学を駆使した次世代抗体医薬の創製. [日本医工学治療学会医工学治療, 23 (3), (2011), 218-223]
浅野竜太郎, 熊谷泉
72) 進化する抗体医薬. [科学評論社リウマチ科, 47 (6), (2012), 700-706]
浅野竜太郎, 熊谷泉
73) ボトムアップな粒子アセンブリツールとしてのバイオ分子. [セラミックス, 47 (8), (2012), 616-619]
梅津光央, 熊谷泉
74) タンパク質フォールドの組換えと次世代抗体医薬. [日経サイエンス, 43 (1), (2013), 108]
熊谷泉
生体機能化学講座（タンパク質工学分野）
0 – 23

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