Technical Information

Technical Information about -NAD+
Update: April 15, 2014 CT
N
O
O
N
+
N
N
OH OH
O
O
P
P
O-
O
O
OH
NH2
N
N
O
OH OH
O
-NAD+
Abbreviation:
Formula
CAS No.
Molecular Weight
UV
BIOLOG Cat. No.
C23H27N7O14P2
(free acid)
[38806-38-1]
687.5
(free acid)
max 265 nm /  10200 / pH 7
N 010
Name: - Nicotinamide- 1, N6- ethenoadenine dinucleotide
Description: In -NAD+ two nucleotides, one containing an adenine nucleobase and the other one containing nicotinamide, are
connected through their phosphate groups. In addition, both the N1 and the N6 nitrogen atoms in the adenine nucleobase are connected
by an etheno bridge forming a tricyclic ring system.
Properties: Fluorescent analogue of -NAD+ with exc 300 nm and em 410 nm. -NAD+ can be used for a proof of ADP-ribosylation,
for fluorometric assays and for preparation of fluorescent poly-ADP-ribose.
Specification: Lyophilized or crystallized sodium salt. The free acid or other salt forms are available upon request. Equal
concentrations of -NAD+ can appear very different in volume due to sensitivity of the lyophilized form to humidity. The compound can
even contract to small volume droplets. Normally the product is located in the conical bottom of the tube. Micromolar quantities are
determined by UV at max.
Purity: Typical analysis is better than 95% (HPLC / UV / 265 nm). The product is not sterile and has not been tested for endotoxins.
Solubility: -NAD+ has good solubility in water (≥ 60 mM). Please rinse tube walls carefully and preferably use ultrasonic or vortex to
achieve total and uniform mixing. When opening the tube please make sure that no substance is lost within the cap.
Stability and Storage: -NAD+ has limited stability at ambient temperature. The compound should be protected from light and
stored in the freezer (-20° Celsius necessary, -80° recommended), for longer storage periods preferably in freeze-dried form.
Toxicity and Safety: Since -NAD+ has multiple tasks in every organism, it is very likely that its analogues will interfere with many
cell regulation processes in vivo. -NAD+ can cause serious irritation to eyes, skin, and respiratory/gastrointestinal tract. Avoid contact
with eyes and skin or ingestion. Product is to be handled with care by trained laboratory personnel only.
Our products are designed, developed and sold for research purposes only! They are intended for in vitro and nonhuman in vivo
laboratory applications. Any other use requires approval of health authorities.
Not for drug, household or related uses!
Material Safety Datasheet available on request.
Selected References for -NAD+:
Schmid, F.; Bruhn, S.; Weber, K.; Mittrücker, H.-W.; Guse, A.H., FEBS Lett., 585, 3544 - 3548 (2011): "CD38: A NAADP
Degrading Enzyme"
Preugschat, F.; Tomberlin, G.H.; Porter, D.J.T., Arch. Biochem. Biophys., 479, 114 - 120 (2008): "The Base Exchange Reaction
of NAD+ Glycohydrolase: Identification of Novel Heterocyclic Alternative Substrates"
Shirato, M.; Tozawa, S.; Maeda, D.; Watanabe, M.; Nakagama, H.; Masutani, M., Biochem. Biophys. Res. Commun., 355, 451 456 (2007): "Poly(Etheno ADP-Ribose) Blocks Poly(ADP-Ribose) Glycohydrolase Activity"
Page 1 of 2
BIOLOG Life Science Institute, Bremen, Germany
Phone: 49 (0) 421 591355
Copyright April 14 by BIOLOG Life Science Institute
Fax: 49 (0) 421 5979713 e-mail: [email protected]
Song, E.-K.; Park, H.-J.; Kim, J.-S.; Lee, H.-H.; Kim, U.-H.; Han, M.-K., J. Biochem. Biophys. Methods, 63, 161 - 169 (2005): "A
Novel Fluorometric Assay for ADP-ribose Pyrophosphatase Activity"
Krebs, C.; Koestner, W.; Nissen, M.; Welge, V.; Parusel, I.; Malavasi, F.; Leiter, E.H.; Santella, R.M.; Haag, F.; Koch-Nolte, F.,
Anal. Biochem., 314, 108 - 115 (2003): "Flow Cytometric and Immunoblot Assays for Cell Surface ADP-Ribosylation Using a
Monoclonal Antibody Specific for Ethenoadenosine"
Schweitzer, K.; Mayr, G.W.; Guse, A.H., Anal. Biochem., 299, 218 - 226 (2001): "Assay for ADP-Ribosyl Cyclase by ReversePhase High-Performance Liquid Chromatography"
Basso, L.A.; Engel, P.C.; Walmsley, A.R., Biochim. Biophys. Acta, 1340, 63 - 71 (1997): "Kinetic Studies on the Binding of 1, N6Etheno-NAD+ to Glutamate Dehydogenase from Clostridium symbiosum"
Klebl, B.M.; Pette, D., Anal. Biochem., 239, 145 - 152 (1996): "A Fluorometric Assay for Measurement of Mono-ADPRibosyltransferase Activity"
Favilla, R.; Mazzini, A., Biochim. Biophys. Acta., 788, 48 - 57 (1984): "The Binding of 1,N6-etheno-NAD to Bovine Liver
Glutamate Dehydrogenase"
Barrio, J.R.; Secrist, J.A.; Leonard, N.J., Proc. Nat. Acad. Sci. USA, 69, 2039 - 2042 (1972): "A Fluorescent Analog of
Nicotinamide Adenine Dinucleotide"
Page 2 of 2
BIOLOG Life Science Institute, Bremen, Germany
Phone: 49 (0) 421 591355
Copyright April 14 by BIOLOG Life Science Institute
Fax: 49 (0) 421 5979713 e-mail: [email protected]

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