Biofilm on orthodontic retention wires - an in vitro and in vivo study - Marije A. Jongsma Publication of this thesis was sponsored by: - Prof. K.G. Bijlstrastichting - Nederlandse Vereniging van Orthodontisten (NVvO) - Ortholab B.V. - Dentsply Lomberg B.V. - Orthodontisch Laboratorium Friesland B.V. - Noord Negentig accountants en belastingadviseurs Biofilm on orthodontic retention wires - an in vitro and in vivo study Door Marije Albertine Jongsma Universitair Medisch Centrum Groningen, Rijksuniversiteit Groningen Groningen, Nederland Cover and layout: MidasMentink.nl Copyright © 2015 by Marije A. Jongsma Printed by: Gildeprint ISBN (printed version) 978-94-6108-923-6 ISBN (electronic version) 978-94-6108-924-3 Biofilm on orthodontic retention wires - an in vitro and in vivo study - Proefschrift ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen op gezag van de Rector Magnificus prof. dr. E. Sterken en volgens besluit van het College van Promoties. De openbare verdediging zal plaatsvinden op woensdag 1 april 2015 om 14.30 uur door Marije Albertine Jongsma geboren op 3 februari 1986 te Den Helder Promotores Prof. dr. Y. Ren Prof. dr. ir. H.J. Busscher Prof. dr. H.C. van der Mei Beoordelingscommissie Prof. dr. S.K. Bulstra Prof. dr. J.M. ten Cate Prof. dr. H. He Aan mijn ouders Paranimfen Leontine A. Jongsma Monique J.M. Vink-Vos CONTENTS Chapter 1 General introduction and aim of the thesis 9 Chapter 2 Orthodontic treatment with fixed appliances and 15 biofilm formation - a potential public health threat? Clinical Oral Investigations (2013) 17:1209-1218 Chapter 3 Biofilm formation on stainless steel and gold wires for 33 bonded retainers in vitro and in vivo and their susceptibility to oral antimicrobials. Clinical Oral Investigations (2013) 17:1209-1218 Chapter 4 In vivo biofilm formation on stainless steel bonded-retainers during different regimens of oral health care. 53 International Journal of Oral Science (2015) doi:10.1038/ijos.2014.69 Chapter 5 Stress relaxation analysis facilitates a quantitative approach towards antimicrobial penetration into biofilms. 73 PLoS One (2013) 8:e63750 Chapter 6 Synergy of brushing mode and antibacterial use on in 95 vivo biofilm formation Submitted to: Journal of Dentistry Chapter 7 General discussion109 Summary 115 Nederlandse samenvatting121 Dankwoord (acknowledgements)129 Curriculum Vitae135
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