Pouyan Boukany, Curriculum vitae Pouyan Boukany i. Personal details Address: Dept. of Chem. Eng.; Product. Process. Eng. Faculty of Applied Sciences Delft University of Technology Julianalaan 136, 2628 BL Delft, The Netherlands Phone: +31-(0)15-2789981 Email: [email protected] Website http://cheme.nl/ppe/people/boukany.shtml ii. Education ● Ph.D. University of Akron, Ohio, USA (Polymer Science, Dec 2008) Nonlinear Flow Behaviour of Entangled DNA Fluids (Advisor: Prof. Shi-Qing Wang) ● M.Sc. Isfahan University of Technology, Iran (Chemical Engineering, Sep 2002) Surface characterization of cotton fabrics coated with polymers iii. Research Experience and Employment • Nov 2011 – now : Assistant Professor of Product & Process Engineering at Delft University of Technology, Faculty of Applied Sciences, Department of Chemical Engineering • July 2013-Sep 2013: Summer Internship at Leiden University Medical Center (LUMC) with prof. Rob Hoeben for training and handling of Genetically Modified Organism (GMO) • Jan 2009 – Oct 2011: Postdoctoral Fellow at Ohio State University, Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Columbus, Ohio, USA • Sep 2004 – Dec 2008: Ph.D. student in the Polymer Science at University of Akron, Ohio, USA iv. Highlights in publications I have published 25 research articles (peer-reviewed) and two review articles, many first-author, leading to h-index of 14. These articles have been cited 358 times, in total. v. Supervision of junior scientists • I have mentored three different PhD students to a successful completion of their project (O. Hemminger, B. Henslee, A. Morss) at Ohio State University (USA). Additionally, I am currently coaching two Post Doctoral Researchers (Dr. Yogesh Harshe, and Dr. Piotr Glazer) within PPE group at TU-Delft. In addition, I am supervising two B.Sc. students, four M.Sc. students and one PhD student at TU-Delft. Furthermore, I have been involved in coaching six B.Sc. students working on their thesis at Ohio State University and University of Akron (Ohio, USA). • Member of five Masters’ and two Bachelor’s thesis committees (2012) • Member of three Ph.D. dissertation proposal committees (2012) vi. Professional membership • Dec/2011-present: • Sept/2005-present: • Sept/2006-present: • Sept/2008-present: • Nov/2011-present: Active member of Soft Matter Discussion at TU Delft Active member of Society of Rheology (SOR) Active member of American Physical Society (APS) Active member of American Institute of Chemical Engineering (AIChE) Active member of Biophysical Society (BPS) vii. Other academic activities • Teaching activities o Research Practicum; Microfluidic Droplets (B.Sc., Fall 2012) o Reactor and Kinetics (M.Sc., Winter 2012) • Reviewer activities o Review for Physical Review Letters, Journal of Rheology, Macromolecules, … (since 2009) • International conferences and workshops (selected) o Biophysical Society Meeting (2007) o Active member of American Institute of Chemical Engineering (2010) o International Congees on Rheology (2008) • Invited seminars (selected) Pouyan Boukany, Curriculum vitae • • • o IEEE Engineering in Medicine and Biology EMBC (San Diego, CA, 2012) o Johns Hopkins University (Baltimore, MD, USA, 2011) o National Institute of Standard (NIST) (Gaithersburg, MD, USA, 2011) o Delft University of Technology (Delft, the Netherlands, 2011) o The Ohio State University (Columbus, OH, USA, 2010) Organizing committee Delft-‐Soft Matter Discussion in January 2013 Organizing committee Process Technology Institute in Delft, November 2012 Organizing committee Bio-‐Day at TU-‐Delft in June 2014 viii. Scientific collaboration I collaborate with numerous colleagues within Delft University (e.g., prof. Kreutzer, prof. Schmidt-Ott, prof. Sudholter, prof Dekker, prof. Kleijn, Dr. van Ommen, Dr. Mendes, Dr. Joo, Dr. Tighe). Additionally my main external collaborations are detailed below: • Prof. Guido Marcucci, (College of Medicine, The Ohio State University) Non-viral delivery of mir181a into Leukemia cancer cells by nano-electroporation; • Prof. Rob Hoeben (Leiden University Medical Center, Cell Biology Dept.) Producing non-viral IPSCs via electroporation • Prof. Shulin Li (The University of Texas Medical Center) in-vivo gene delivery via high-throughput nano-electroporation • Prof. Kam Leong (Duke University, USA) Intra Cellular Trafficking by QD-FRET visualization; • Prof. Gregory Lafyatis (The Ohio State University) Optical Tweezers for single force measurements • Prof. Rashid Bashir (University of Illinois Urbana Champion) Electrical measurement through solid state nanopores; • Prof. James Lee (The Ohio State University, USA) Micro/nano-devices for biomedicine; • Prof. Shi-Qing Wang (University of Akron, USA) Molecular Rheology; • Prof. Ronald Larson (University of Michigan, USA) Physics of soft matter, • Dr. Sebastian Shwind (The University of Hospital Leipzig, Germany) MicroRNAs delivery of cancer therapy by nano-fluidics; ix. Prizes and awards • American Institute of Chemists Outstanding Postdoctoral Award, (Spring 2011) • Best paper award for postdoctoral research competition in Society of Rheology, (Fall 2009) • Postdoctoral Fellowship, National Science Foundation (NSF) Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, (2009-2011) • Finalist for the Frank J. Padden Award for Excellence in Polymer Physics Research, (APS, 2008) • Finalist for the Bausch & Lomb Award for Innovative Research, (Summer 2008) • Travel Award for Society of Rheology, (Fall 2007) • Graduate Scholarship, The University of Akron, Polymer Science Department (2004-‐2008) x. Funding ID o o o Oct.2013-Oct-2018: European Research Council (ERC) Starting Grant (ERC-STG): Engineering of DNA transport into living cells by precision in Electroporation (PI, 1.5 M€) Oct.2012-‐Oct-‐2016: Marie Curie Carrier Integration Grant (CIG): Single Cell Electroporation and DNA dynamics: from Bulk to Nano-‐fluidics (PI, 100 k€) Aug.2012-‐Aug-‐2015: Nano-‐NextNL Project (10C): the application of micro-‐devices for chemical processing and molecular detection (PI, 150 k€) Pouyan E. Boukany, Curriculum Vitae xi. Publication Journals: h-index: 14, Sum of the times cited: 358, Citing articles: 177, Average citation per item:12.24. (Source: ISI web of science) Papers Published or In Press: [Impact factor] {Number of citations} 27. P.J. Glazer, L. Bergen, L. Jennings, A. J. Houtepen, E. Mendes and Boukany, P.E.,* “ Generating Aligned Micellar Nanowire Arrays by Dewetting of Micro-patterned Surfaces ” Small, (2014). [Impact: 7.6] 26. Boukany, P., Wu, Y., Zhao, X., Leong, K., Lee, L.J., “Nano-channel Electroporation changes cellular uptake and realease of lipoplex nanoparticles” Advanced Healthcare Mat. (2013). 25. Ravindranath, S., Wang, Y., Boukany, P., Li, X., “Letter to the editor: Cone partitioned plate (CPP) vs circular couette” J. Rheol 56, 675 (2012). [Impact:3] 24. Boukany, P. E.; Morss, A.; Zhang, X.; Bo, Y.; Henslee, B.; Wang, X.; Wu, Y.; Hu, X.; Lafyatis, G.; Lee, L. J.; “Nanochannel electroporation delivers precise amounts of biomolecules into living cells” Nature Nanotech. 6,747-754 (2011). [Impact:27] {Cited:18} 23. Hu, X.; Boukany, P. E.; Hemminger, O.; Lee, L. J. “The use of microfluidics in rheology” Macromol. Mater. Eng. 296, 308-320 (2011). [Impact:2] {Cited:3} 22. Wang, S.Q.; Ravindranath, S.; Boukany, P. E.; “Homogenous shear, wall slip and shear banding of entangled polymeric liquids in simple-shear rheometry: a road Map of non-linear rheology” Macromolecules 44, 183-190 (2011). [Impact: 5] {Cited:9} 21. Lim, Y.; Boukany, P. E.; Farson, D.; Lee, L. J. “Direct-write femtosecond laser ablation and dna combing and imprinting for fabrication of a micro/nanofluidic device on an ethyene glycol dimethacrylate polymer” J. Micromech. Microeng. 21, 015012 (2011). {Cited: 4} 20. Boukany, P. E.; Hemminger, O.; Wang, S. Q.; Lee, L. J. “The molecular imaging of slip in entangled DNA solution” Phys. Rev. Lett. 105, 027802 (2010). [editor’s suggestions] [Impact 7.5] {Cited: 11} 19. Guan, J.; Boukany, P. E.; Chiou, N.; Hemminger, O.; Lee, L. J. “The large area of laterally ordered nanochannel arrays from DNA combing and imprinting ” Adv. Mater. 22, 3997-4001 (2010). [Impact 14] {Cited:8} 18. Boukany, P. E.; Wang, S. Q. “Shear banding or not in entangled DNA solutions” Macromolecules 43, 6950-6952 (2010). [Impact 5] {Cited:5} 17. Hemminger, O.; Boukany, P. E.; Wang, S. Q.; Lee, L. J.; “Flow pattern and molecular visualization of DNA solutions through a 4:1 planar micro-contraction” J. Non-Newtonian Fluids. 165, 1613-1624 (2010). {Cited:5} 16. Zimmerman, L. B.; Rauscher, M. D.; Ellis, J.; Boukany, P.; Lee, J. L. “Nanoimprinting using self assembled ceramic nanoislands” Nanotechnology 21, 045304 (2010). [Impact 3.6] 15. Boukany, P. E.; Wang, S. Q.; “Nature of steady flow in entangled fluids revealed by super imposed oscillatory shear” J. Rheol. 53, 1425-1435 (2009). [Impact 3] {Cited: 6} 14. Boukany, P. E.; Wang, S. Q.; Wang, X. “Step shear of entangled linear polymer melts: New experimental evidence for elastic yielding” Macromolecules 42, 6261-6269 (2009). [Impact:5] {Cited: 16} 13. Boukany, P. E.; Wang, S. Q.; Wang, X. “Universal scaling behavior of startup shear of entangled linear polymer melts” J. Rheol. 53, 617-629 (2009). [Impact:3] {Cited: 22} 12. Boukany, P. E.; Wang, S. Q. “Exploring the origin of interfacial yielding during and after shear” Macromolecules 42, 2222-2228 (2009). [Impact:4.8] {Cited: 12} 11. Boukany, P. E.; Wang, S. Q. “Exploring transition from wall slip to bulk shearing banding in wellentangled DNA solutions” Soft Matter 5, 780-789 (2009). [Impact:4.5] {Cited: 11} 10. Boukany, P. E.; Wang, S. Q. “Shear banding or not in entangled DNA solutions depending on the level of entanglement” J. Rheol. 53, 73-83 (2009). [Impact:3.1] {Cited: 16} 9. Boukany, P. E.; Hu, Y. T.; Wang, S. Q. “Observations of wall slip and shear banding in an entangled DNA solution” Macromolecules 41, 2644-2650 (2008). [Impact:4.8] {Cited: 28} 8. Boukany, P. E.; Wang, S. Q. “Use particle tracking velocimetry and flow birefringence to study nonlinear flow behavior of entangled worm-like micellar solutions: From wall slip to bulk disentanglement and chain scission” Macromolecules, 41, 1455-1464 (2008). [Impact:4.8] {Cited: 15} 7. Wang, Y.; Boukany, P.; Wang, S. Q.; Wang, X. “Elastic breakup in uniaxial extension of entangled polymer melts” Phys. Rev. Lett. 99, 237801 (2007). [Impact:7.6] {Cited: 34} Pouyan E. Boukany, Curriculum Vitae 6. Wang, S. Q.; Rvindranath, S.; Wang, Y.; Boukany, P.; “New theoretical consideration in polymer rheology: Elastic breakdown of chain entanglement network” J. Chem. Phys. 127, 064903 (2007). [Impact:2.9] {Cited: 44} 5. Boukany, P. E.; Wang, S. Q. “A correlation between velocity profile and molecular weight distribution in sheared entangled polymer solutions” J. Rheol. 51, 217-233 (2007). [Impact:3.1] {Cited: 35} 4. Wang, S. Q.; Rvindranath, S.; Boukany, P.; Olechnowics, M.; Quirk, R. P.; Halasa, A.; Mays, J. “Nonquiescent relaxation in entangled polymeric liquids after step shear” Phys. Rev. Lett. 97, 187801 (2006). [Impact:7.6] {Cited: 43] 3. Boukany, P. E.; Tapadia, P.; Wang, S. Q. “Interfacial stick-slip transition in simple shear of entangled melts” J. Rheol. 50, 641-654 (2006). [Impact:3.1]{Cited:19} 2. Zadhoush, A.; Alsharif, M.; Boukany, P. E. “The influence of K-value and plasticizer type on the rheological behaviour of plastisol used in coated fabrics” Iran. Polym. J. 5, 371-379 (2004). 1. Mortazavi, S. M.; Boukany, P. E. “Application of mixtures of resin finishing to achieve some physical properties on interlining cotton fabrics: I-Effect of stiffening and cross-linking agents” Iran. Polym. J. 3, 213-218 (2004). - Peer reviewed conference Proceedings • Boukany, P. E.; Nano-Channel electroporation for precise delivery and detection of biomolecules, 2012 IEEE Engineering in Medicine and Biology EMBC (San Diego, CA, USA) Boukany, P. E.; Wang, X.; Yu, B.; Wu, Y.; Lee, L. J.; “Using Nanoelectroporation, Molecular Beacons and Single Cell PCR to Quantify the Relationship Between Sirna/MicroRNA Delivery and Gene Silencing In Cancer Cells” Minneapolis, MN, USA, A.I.Ch.E., 2011; Paper 439g • • Boukany, P. E.; Wu, Y.; Leong, K.; Lee, L. J.; “Using nanoelectroporation to understand intracellular trafficking of nanoparticles In gene delivery” Minneapolis, MN, USA, A.I.Ch.E., 2011; Paper 504c • Boukany, P. E.; Morss, A., Zhang, X. Yu, B., Hu, X.; Lee, L. J.; “Nanoelectroporation (NEP) for precise drug/gene delivery” Salt Lake City, UT, USA, A.I.Ch.E., 2010; Paper 294f • Boukany, P. E.; Wang, S. Q., Lee, L. J.; “Molecular Imaging of Shear Inhomogeneity (Wall slip/Shear Banding) in Entangled Fluids” Salt lake city, UT, USA, A.I.Ch.E., 2010; Paper 237g. • Boukany, P. E.; Morss, A., Zhang, X. Yu, B., Hu, X.;Lee, L. J.; “Real-time detection and imaging of miRNA/proteins in individual cells by nanoelectroporation (NEP)” Salt lake city, UT, USA, A.I.Ch.E., 2010; Paper 604a • Boukany, P. E.; Wang, S. Q., Lee, L. J.; “Using real-time DNA imaging/Rheometry to demonstrate the molecular mechanism of wall slip in entangled fluids: Interfacial chain disentanglement versus chain desorption” Santa Fe, New Mexico, USA, Society of Rheology (SOR), 2010. • Boukany, P. E.; Hemminger, O.; Hu, X.; Fei, Z.; Chiou, N.; Lee, L. J.; “A nanochannel/microwell array device for controlled gene Delivery to individual cells by localized electroporation” Nashville, TN, USA, A.I.Ch.E., 2009; Paper 577a • Chiou, N.; Boukany, P. E.; Hemminger, O.; Bo, Y.; Lee, L. J.; “Ion transport in nanochannels fabricated by DNA combing and imprinting” Nashville, TN, USA, A.I.Ch.E., 2009; Paper 591g • Hemminger, O.; Boukany, P. E.; Hu, X.; Chiou, N.; Lee, L. J.; “Electrokinetically driven DNA dynamics in nanochannel/microwell array” Nashville, TN, USA, A.I.Ch.E., 2009; Paper 141f 4 Pouyan E. Boukany, Curriculum Vitae • Boukany, P. E.; Hemminger, O.; Wang, S. Q.; Lee, L. J. “Molecular imaging of wall slip and shear banding in entangled DNA solutions” Madison, Wisconsin, USA, October 18-22, Society of Rheology (SOR), 2009. • Boukany, P. E.; Hemminger, O.; Wang, S. Q.; Lee, L. J. “Understanding the origin of flow inhomogeneity in entangled fluids by direct visualization of individual DNAs during flow” Madison, Wisconsin, USA, October 18-22, Society of Rheology (SOR), 2009. • Boukany, P. E.; Wang, S. Q.; “What do we learn rheologically from DNA solutions?” Monterey, California, August 3-8, International Congress of Rheolgoy (ICR), 2008; Paper BR7 • Boukany, P. E.; Wang, S. Q.; “Use DNA solutions to model polymer entanglement in flow: simultaneous rheometric and particle-tracking velocimetric measurements” New Orleans, Louisiana, American Physical Society (APS), 2008; Paper J18.001 (Padden Award Finalist for Excellent in Polymer Physics) • Hu, Y. T.; Boukany, P. E.; Lips, A.; Wang, S. Q.; “Shear banding in entangled DNA solutions” Salt lake city, UT,USA, A.I.Ch.E., 2007; Paper 313i • Boukany, P. E.; S. Q. Wang, “Step shear in entangled polymer melts: from interfacial to bulk failure at large shear deformation,” Portland, MA, USA, Society of Rheology (SOR), 2007. • Boukany, P. E.; Wang, S. Q.; “Disentanglement behavior of DNA and wormlike micellar solutions as probed with particle tracking velocimtery” Denver, Colorado, March 5-9, American Physical Society (APS), 2007; Paper P30.006 • Boukany, P. E.; S. Q. Wang, “Probing the disentanglement of DNA solution by PTV,” Portland, MA, USA, October 9-12, Society of Rheology (SOR), 2006. • Boukany, P. E.; S. Q. Wang, “Probing the breakdown of chain entanglement in simple shear: The effect of molecular weight distribution,” Baltimore, MD, USA, American Physical Society (APS), 2006; Paper Z24.11 Professional activities • Dec/2011-present: • Sept/2005-present: • Sept/2006-present: • Sept/2008-present: • • • • • • Active member of Soft Matter Discussion at TU Delft Active member of Society of Rheology (SOR) Active member of American Physical Society (APS) Active member of American Institute of Chemical Engineering (AIChE) Nov/2011-present: Active member of Biophysical Society (BPS) Jan/2010-present: Collaboration with Professor Kam Leong, Biomedical Eng., Duke University, Durham, NC, USA Jan/2010-present: Collaboration with Professor Rashid Bashir, Electrical Eng., University of Illinois Urbana Champion, Urbana, IL, USA Nov/2009-Oct/2011: Collaboration with Professor Guido Marcucci, College of Medicine (Oncology), The Ohio State University, USA Feb/2009-Oct/2011: Collaboration with Profs. Sooryakumar and Lafyatis, Physics Dept.,The Ohio State University, USA Jan/2009-Oct/2011: Team leader of multiple research projects in the NSEC at The Ohio State University, USA, Professor L. James Lee 5 Pouyan E. Boukany, Curriculum Vitae • Sep/2004-Dec/2008: Team leader of multiple research projects in Shi-Qing Wang’s lab in Polymer Science Dept., University of Akron, USA All of these collaborations have been successful and led to joint publications (some of which are currently under review or in preparation). SCIENTIFIC EXPERTISE Molecular biology techniques (PCR, gel electrophoresis, cloning, Gene Transfection) Laboratory biochemistry (thin layer chromatography, spectroscopy) Optical trapping instrumentation TIRF microscopy instrumentation Single molecule kinetics Programming (Labview, Matlab, HTML) Optical microscopy, advanced imaging Laboratory electronics SELECTED TECHNICAL SKILLS • Micro/Nano-Fabrication and Manipulation: Photolithography High Precision Micro-Milling Soft Lithographic Techniques Nano-imprinting Molecular Combing Surface Treatment Micro/Nanofluidics Reactive-Ion-Etching • Micro/Nano-Characterization: Scanning Electron Microscopy Atomic Force Microscopy Fluorescence Microscopy Thermal Analysis Techniques Dynamic Light Scattering Optical Profilometry Instron Mechanical Testing FTIR Spectroscopy • Biological Assays: Cell Culture Cell Viability Gene Transfection Electroporation DNA Functionalization Bio-molecular Staining Confocal Microscopy Gel Electrophoresis PCR, Western Blot • Flow Visualization of Complex Fluids: Molecular Imaging Fluorescent Imaging Rheometric/PTV Rheo-optical measurement measurements Particle Image Velocimetry Micro/nanofluidics 6
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