California Mastitis Test Scoring Symbol SuggestedDescription of visible reaction meaning Many new mastitis infections occur shortly before or on the day of calving. Incorporate the California Mastitis Test (CMT) to screen fresh cows for subclinical mastitis infections. NNegative Mixture remains liquid, homogeneous, with no evidence of thickening. TTrace The slight thickening that forms is seen best by tipping the paddle back and forth and observing the mixture as it flows over the bottom of the cup. Trace reactions tend to disappear with continued rotation of the paddle. 1 A distinct thickening of the liquid forms, but there is no tendency toward gel formation. With some milk, the thickening may disappear after rotating the paddle more than 20 seconds. 2Distinct positive Mixture thickens immediately, and a gel formation is suggested. As the mixture is swirled, it tends to move toward the center, exposing the bottom of the outer edge of the cup. When the motion is stopped, the mixture levels out and covers the bottom of the cup. 3 gel is formed, which causes the A surface to look like a partially fried egg. Usually you’ll see a central peak that remains projecting above the mass, even after the rotation of the paddle is stopped. guidelines include: • Use the CMT on the first or second day after calving to catch any new infections as soon as possible • Compare the status of each quarter with CMT scores recorded at dry off to distinguish new infections from old ones • Culture individual quarters that score CMT 2 or higher • Culture, but do not treat, old, chronic quarters that did not clear during the dry period • Based upon culture results and veterinarian recommendations, treat appropriate quarters with an approved lactating antibiotic To ensure more uniform results when reading CMTs, proper screening techniques must be established. Use the following guide to help train employees on CMT use and scoring. Weak positive 7 steps to achieve an accurate CMT 1. Strip four streams of foremilk before stripping into CMT paddle cups. 2. Strip approximately 1/2 teaspoon of milk from each quarter into the respective CMT paddle cups. 3. Add an equal amount of CMT reagent to each cup. 4. Rotate the paddle in a circular motion to thoroughly mix the contents. 5. Read the test in approximately 10 seconds while still rotating. 6. Interpret CMT scores using chart at right. 7. Rinse the paddle thoroughly with water before taking the next test. www.PfizerAH.com All brands are the property of Pfizer Inc., its affiliates and/or its licensors. ©2011 Pfizer Inc. All rights reserved. GDR11062 Strong positive Source: NebGuide G81-556-A, University of Nebraska Cooperative Extension
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