Product Datasheet GST tag ELISA Kit ab126581 4 Images Overview Product name GST tag ELISA Kit Detection method Colorimetric Precision Intra-assay Sample n Mean SD CV% Overall 8.5% Overall 8.5% Tests 1 x 96 well plate Sample type Cell Lysate Assay type Sandwich (quantitative) Sensitivity 1 ng/ml Assay duration Multiple steps standard assay Product overview Abcam’s Gluthathione S-Transferase (GST) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of GST tagged protein expression in cell extracts or after purification. The assay employs a GST specific antibody coated onto a well plate strips. Standards and samples are pipetted into the wells and GST present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a primary anti- GST detector antibody is added. After washing away unbound detector antibody an HRP-conjugated secondary detector antibody (HRP Label) specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GST bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm. Notes Typical working ranges GST (pure protein) 1 – 200 ng/mL GST fusion protein 6 – 400ng/mL Gluthathione S-transferase (GST) is a 26 kDa protein typically used as a fusion partner of recombinant proteins to increase the level of protein expression in bacterial systems, enhance solubility, protect from proteolysis, improve folding and allow purification by affinity chromatography on gluthathione resin. This GST ELISA Kit is an enzyme immunoassay developed for the sensitive detection and quantitation of GST or GST fusion proteins in cells samples or purified tagged proteins. The quantity of GST can be determined in lysates or intermediate purification fractions by comparing their absorbance with that of a known recombinant GST standard curve. The kit has a detection sensitivity limit of 1 ng/mL of GST. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and GST fusion proteins. Tested applications ELISA Platform Microplate Properties Storage instructions Store at +4°C. Please refer to protocols. Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X GST Detector Antibody 1 x 700µl 10X HRP Label 1 x 1ml 20X Buffer 1 x 20ml http://www.abcam.com/GST-tag-ELISA-Kit-ab126581.html 1 Product Datasheet Extraction Buffer 1 x 15ml GST Microplate (12 x 8 antibody coated well strips) 1 unit GST Standard 1 vial TMB Development Solution 1 x 6ml Cellular localization Cytoplasmic Applications Our Abpromise guarantee covers the use of ab126581 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes ELISA Use at an assay dependent concentration. GST tag ELISA Kit images Example standard curve. GST standard protein was serially diluted from 400 ng/mL to 6 ng/mL. Background was subtracted from data points prior to log transformation and curve fitting (log-agonist vs. response – variable slope). Sensitivity was calculated mathematically to be as low as 1 ng/mL. ELISA- GST tag ELISAKit (ab126581) Dynamic Range of a recombinant protein tagged to GST. Recombinant full length protein (Human) p53 with N terminal GST tag (ab43615) was tested in a dilution series from 400 ng/mL to 12 ng/mL using the GST ELISA kit. Background was subtracted from data points prior to log transformation and curve fitting (log-agonist vs. response – variable slope). ELISA- GST tag ELISAKit (ab126581) All lanes : GST tag ELISAKit (ab126581) Lane 1 : GST recombinant protein (ab89494) Lane 2 : p53 tagged to GST protein (ab43615) Western blot - GST tag ELISAKit (ab126581) http://www.abcam.com/GST-tag-ELISA-Kit-ab126581.html 2 Product Datasheet All lanes : Anti-GST antibody (ab19256) Lane 1 : GST recombinant protein (ab89494) Lane 2 : p53 tagged to GST protein (ab43615) Western blot - GST tag ELISAKit (ab126581) Please note: All products are "FOR RESEARCH USE ONLYAND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery** Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors **Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand, which operate a 120 day guarantee. Please contact your regional office for further details Visit us at: www.abcam.com http://www.abcam.com/GST-tag-ELISA-Kit-ab126581.html 3
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