Automated On-Line Extraction System for MS/MS Newborn Screening Newborn screening is a public health program provided by most of the countries around the world aimed at screening newborns for a list of serious genetic and metabolic disorders. Early diagnosis of those conditions can help prevent their further development which untreated often results in brain damage, organ damage, and even death. A routine neonatal screening procedure requires that a health professional takes a few drops of blood from the baby’s heel, applies them onto a filter paper and sends such prepared samples to a laboratory for a number of analytical tests. Sample preparation taking place before analysis may be laborious, time consuming and not very precise due to carry-over when processed with traditional “punch-and-elute” methodology. Application of CAMAG DBS-MS 500 direct sample elution system offers fast, efficient and highly reliable sample handling. In addition, this fully automated system eliminates the need for any human inference (optional phrase: operates without any need for any manual work) in-between samples. Screening for Phenylketonuria (PKU)* Normal Routine NBS IS-Phe Phenylalanine Phe Phe PKU CAMAG DBS-MS 500 IS-Phe Phe R2 = 0.994 IS-Phe PKU Routine NBS Intra-day Precision [%CV, n = 10] Intra-day Accuracy [%, n = 10] Inter-day Precision [%CV, n = 3] Inter-day Accuracy [%, n = 3] Concentration (µmol/L) CAMAG NBS (direct analysis) Concentration (µmol/L) CAMAG NBS (direct analysis) Concentration (µmol/L) CAMAG NBS (direct analysis) Concentration (µmol/L) CAMAG NBS (direct analysis) Low 4.9 Low 117.7 Low 8.3 Low 111.5 High 3.3 High 114.4 High 7.0 High 103.8 Screening for Medium chain acyl-CoA dehydrogenase deficiency (MCAD)* Screening for Propionic acidemia (PA)* IS-C8 Normal Routine NBS Normal Routine NBS C3 C3 C8 MCAD CAMAG DBS-MS 500 ChromSystems IS-C3 PA CAMAG DBS-MS 500 ChromSystems IS-C8 C8 IS-C3 C3 IS-C8 MCAD Routine NBS C8 * Data provided by Dr. Ralph Fingerhut, Universitäts-Kinderspital Zürich PA Routine NBS IS-C3 CAMAG DBS-MS 500 FULLY AUTOMATED DRIED BLOOD SPOT EXTRACTION SYSTEM       FULLY AUTOMATED HANDLING OF UP TO 500 DBS CARDS BARCODE READING DEVICE FOR DBS CARD IDENTIFICATION AUTOMATED INTERNAL STANDARD (IS) APPLICATION MODULE EXTRACTION MODULE INDEPENDENT OF LC-MS MOBILE PHASE WASH STATION TO ELIMINATE CARRY-OVER EASY INTEGRATION WITH ANY LC-MS SYSTEM CAMAG (Switzerland) Sonnenmattstrasse 11 4132 Muttenz +41 61 467 34 34 [email protected] CAMAG (Germany) Bismarckstrasse 27-29 12169 Berlin +49 30 516 555 0 [email protected] CAMAG Scientific (USA) 515 Cornelius Harnett Drive Wilmington, NC 28401 (800) 334 3909 [email protected] www.camag.com/dbs