CATALOGUE CENTRIFUGATION
2014 / 2015
ULTRACENTRIFUGEUSES
ULTRACENTRIFUGEUSE DE TABLE
CENTRIFUGEUSES HAUTE VITESSE
CENTRIFUGEUSES GRANDE CAPACITÉ
CENTRIFUGEUSES
ROTORS
ADAPTATEURS
TUBES
BOUTEILLES
ACCESSOIRES
Brilliance
at every turn.
ULTRACENTRIFUGES
PRECISION-MADE PERFORMANCE.
BR-8101Q
Ultracentrifugation
Without limitation.
Starting with the innovations created by our founder, Arnold O. Beckman Ph.D., Beckman Coulter
has lived at the forefront of the technology that continues to help improve life for all of humankind.
Our foray into the world of ultracentrifugation is no different. In fact, we introduced the first
commercial ultracentrifuge in 1947. Since then, our elite line of ultracentrifuges has attained
incredible levels of speed and sophistication, without sacrificing usability.
Not unlike the rest of our portfolio of products, our ultracentrifugation solutions certainly live up
to the Beckman Coulter name—a name in which laboratories around the world continue to have
the utmost confidence.
www.beckmancoultercentrifugation.com
S
ince the introduction of the first commercial
Rotors with the BioCertified symbol have been tested to
ultracentrifuge in 1947 — the classic Beckman
demonstrate containment of aerosols under normal operating
Model L — Beckman Coulter has been at the
conditions of the associated Beckman Coulter centrifuge
forefront of centrifuge innovation. Although the physics
of this basic separation technique never change, Beckman
when used and maintained as instructed.
Following the centrifuge descriptions, listings of their
Coulter continually designs new and innovative rotors and
rotors are included with information on speed and g-force
accessories, and develops advanced methods that allow
capability. Also included is information on tubes and bottles
the forces of centrifugation to be applied in new ways.
that can be used and the adapters they require.
This centrifuge product selection guide is designed to
Tubes and bottles are cross-referenced in a separate
help you determine the most efficient centrifuge tools for
section which provides details on tube materials, chemical
your laboratory.
compatibility, tube designs, and tube closure options.
Each section begins with a brief description of instru-
A reference section at the back of the guide includes
ments that Beckman Coulter offers within that centrifuge
quick-reference charts on instrument and tube selection,
category. Because biocontainment is a major concern in
as well as frequently used formulas.
today’s laboratories, Beckman Coulter provides a number
of options that address this issue. Special BioSafe instruments and accessories are available across our centrifuge
product line and are identified with the following icons:
BioSafe and BioSafety are terms intended to describe
the enhanced biocontainment features of our products.
BioCertified is a term used to describe our products
which have been tested and validated to demonstrate
containment of microbiological aerosols by an
independent, third-party facility (Health Protection
Agency, Porton Down, UK or USAMRIID,
Ft. Detrick, MD, USA). Improper use or maintenance
may affect seal integrity and, thus, containment.
BioEnhanced is a term intended to describe our
products’ enhanced level of biocontainment by design.
Beckman Coulter Ultracentrifuge
i
www.beckmancoultercentrifugation.com
Table of Contents
Ultracentrifugation
Ultracentrifuge Descriptions
Optima X Series Floor Model Preparative ...................... 1-2
Optima MAX Series Tabletop Preparative ...................... 1-4
Airfuge Air-Driven Micro-Ultracentrifuge ...................... 1-6
Instrument Classifications; Speed Reductions;
Rotor Designations............................................................. 1-7
k Factors; Centrifugal Forces ............................................. 1-7
Tube Kits; Use of Small Tubes; g-Max System ................. 1-7
Quick-Reference Guide to Instrument Classification
Rotor Compatibility ........................................................... 1-8
Beckman Coulter Floor Model Ultracentrifuge Rotors
by Use ................................................................................ 1-9
Rotors for Floor Model Preparative Ultracentrifuges
Fixed-Angle Rotors ....................................................... 1-10
NVT Near-Vertical Tube Rotors .................................... 1-20
Vertical-Tube Rotors...................................................... 1-24
Swinging-Bucket Rotors ............................................... 1-28
Continuous-Flow Rotor ................................................. 1-36
Zonal Rotors .................................................................. 1-37
Rotors for Tabletop Ultracentrifuges
Quick-Reference Guide to Rotor Specifications and
Tabletop Ultracentrifuge Compatibility......................... 1-40
Fixed-Angle Rotors ....................................................... 1-42
NVT Near-Vertical Tube Rotors .................................... 1-54
Swinging-Bucket Rotors ............................................... 1-57
Rotors for Airfuge Air-Driven Micro-Ultracentrifuges
Fixed-Angle Rotors ....................................................... 1-59
Chylomicron Rotor ........................................................ 1-61
Batch Rotor .................................................................... 1-61
Electron Microscopy Particle-Counting Rotor .............. 1-62
ii
Tubes and Bottles
Tubes and Bottles for Every Application ........................... 2-1
Tube Selection Considerations
Compatibility of Tube Material with
Solvents and Sample ....................................................... 2-2
Gradient Formation and Fractionation ............................ 2-2
High-Temperature Centrifugation ................................... 2-2
Tube Sizes ....................................................................... 2-2
Tube Cleaning, Sterilization, and Reuse ......................... 2-2
Tube Closures .................................................................. 2-3
OptiSeal Tubes ................................................................ 2-3
Quick-Seal Tubes ............................................................ 2-3
A Quick Reference Chart to Tube Materials and
Their Properties.................................................................. 2-4
Tubes and Bottles (Listed by Volume) ............................... 2-5
Bottle Assemblies with Three-Piece Caps ......................... 2-8
Tube Cap Assemblies and Parts ......................................... 2-9
Tools, Accessories, and Supplies
Tools and Supplies for Preparative Ultracentrifuge Rotors
Tools for Fixed-Angle Rotors ......................................... 3-2
Tools for Vertical-Tube and NVT Rotors ........................ 3-4
Tools for Swinging-Bucket Rotors .................................. 3-4
Tools for Zonal Rotors .................................................... 3-4
Supplies ........................................................................... 3-5
Tools and Accessories for Quick-Seal Tubes
Tools for Quick-Seal Tubes ............................................. 3-5
Quick-Seal Tube Racks ................................................... 3-6
Tools for OptiSeal Tubes ................................................... 3-6
Cordless Tube Topper ........................................................ 3-7
Tube Slicer—for Preparative Ultracentrifuge Tubes ......... 3-8
CentriTube Slicer—for Micro Ultracentrifuge Tubes ........ 3-8
Rotor Cleaning Kit ............................................................. 3-9
Fraction Recovery System ............................................... 3-10
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Table of Contents
Reference
Guide to Centrifuge Selection............................................ 4-2
How To Choose a Rotor
The Importance of k Factor ............................................. 4-4
Rotor Material ................................................................. 4-4
Swinging-Bucket (SW) Rotors........................................ 4-4
Fixed-Angle (FA) Rotors ................................................ 4-4
Vertical-Tube (VT) Rotors .............................................. 4-4
NVT Near-Vertical Tube Rotors...................................... 4-4
Continuous-Flow/Zonal Rotors ....................................... 4-4
Particle Separation in SW, FA, NVT and VT Rotors ......... 4-5
Preparative Ultracentrifuge Rotor Recommendations
for Key Applications .......................................................... 4-6
Gradient Materials .......................................................... 4-7
Commonly Used Gradient Materials with
Their Solvents ................................................................. 4-8
Density, Refractive Index, and Concentration
Data—CsCl at 25°C ........................................................ 4-9
Density, Refractive Index, and Concentration
Data—Sucrose at 20°C ................................................. 4-10
Density Conversion for Cesium
and Rubidium Salts at 20°C .......................................... 4-11
Useful Formulas ............................................................... 4-12
Beckman Coulter Ultracentrifuge
Support Services
Field Service Support......................................................... 5-2
Field Rotor Inspection Program ......................................... 5-2
Rotor Safety Seminars ....................................................... 5-2
On-Site Rotor Inspection and Maintenance ....................... 5-2
A Comprehensive Approach to
Centrifuge System Care ..................................................... 5-2
iii
www.beckmancoultercentrifugation.com
Ultracentrifugation
1
Ultracentrifuge Descriptions
Optima X Series Floor Model Preparative ................................. 1-2
Optima MAX Series Tabletop Preparative ................................. 1-4
Airfuge Air-Driven Micro-Ultracentrifuge ................................ 1-6
Instrument Classifications; Speed Reductions;
Rotor Designations........................................................................ 1-7
k Factors; Centrifugal Forces ........................................................ 1-7
Tube Kits; Use of Small Tubes; g-Max System ............................ 1-7
Quick-Reference Guide to Instrument Classification
Rotor Compatibility ...................................................................... 1-8
Beckman Coulter Floor Model Ultracentrifuge Rotors
by Use ........................................................................................... 1-9
Rotors for Floor Model Preparative Ultracentrifuges
Fixed-Angle Rotors.................................................................. 1-10
Type 100 Ti ......................................................................... 1-10
Type 90 Ti ........................................................................... 1-11
Type 70.1 Ti ........................................................................ 1-12
Type 70 Ti ............................................................................. 1-13
Type 50.4 Ti ........................................................................ 1-14
Type 50.2 Ti ........................................................................ 1-15
Type 45 Ti ........................................................................... 1-16
Type 42.2 Ti ........................................................................ 1-17
Type 25................................................................................ 1-18
Type 19................................................................................ 1-20
NVT Near-Vertical Tube Rotors .............................................. 1-20
NVT 100 ............................................................................. 1-20
NVT 90 ............................................................................... 1-21
NVT 65.2 ............................................................................ 1-22
NVT 65 ............................................................................... 1-23
Continuous-Flow Rotor
CF 32 Ti .............................................................................. 1-36
Zonal Rotors............................................................................. 1-37
Ti-15 .................................................................................... 1-38
Ti-14 .................................................................................... 1-38
Seal Assembly ..................................................................... 1-39
Rotors for Tabletop Ultracentrifuges
Quick-Reference Guide to Rotor Specifications and
Tabletop Ultracentrifuges Compatibility ............................ 1-40
Fixed-Angle Rotors.................................................................. 1-42
TLA-120.2 .......................................................................... 1-42
TLA-120.1 .......................................................................... 1-43
TLA-110.............................................................................. 1-44
TLA-100.3 .......................................................................... 1-45
TLA-100 ............................................................................. 1-46
TLA-55 ............................................................................... 1-47
MLA-150 ............................................................................ 1-48
MLA-130 ............................................................................ 1-49
MLA-80 .............................................................................. 1-50
MLA-55 .............................................................................. 1-51
MLA-50 .............................................................................. 1-53
NVT Near-Vertical Tube Rotors .............................................. 1-54
TLN-120 ............................................................................. 1-54
TLN-100 ............................................................................. 1-55
MLN-80 .............................................................................. 1-56
Swinging-Bucket Rotors .......................................................... 1-57
TLS-55 ................................................................................ 1-57
MLS-50 ............................................................................... 1-58
Rotors for Airfuge Air-Driven Micro-Ultracentrifuges
Vertical-Tube Rotors ................................................................ 1-24
VTi 90 ................................................................................. 1-24
VTi 65.2 .............................................................................. 1-25
VTi 65.1 .............................................................................. 1-26
VTi 50 ................................................................................. 1-27
Fixed-Angle Rotors.................................................................. 1-59
A-110 .................................................................................. 1-59
A-100/30 ............................................................................. 1-59
A-100/18 ............................................................................. 1-60
A-95 .................................................................................... 1-60
Swinging-Bucket Rotors .......................................................... 1-28
SW 60 Ti ............................................................................. 1-28
SW 55 Ti ............................................................................. 1-29
SW 41 Ti ............................................................................. 1-30
SW 40 Ti ............................................................................. 1-31
SW 32.1 Ti .......................................................................... 1-32
SW 32 Ti ............................................................................. 1-33
SW 28.1............................................................................... 1-34
SW 28.................................................................................. 1-35
ACR-90 Chylomicron Rotor .................................................... 1-61
Beckman Coulter Ultracentrifuge
Batch Rotor .............................................................................. 1-61
EM-90 Electron Microscopy Particle-Counting Rotor ............ 1-62
1-1
www.beckmancoultercentrifugation.com
Ultracentrifugation
Optima X Series Floor Model Preparative Ultracentrifuges
Experience the visibility and control of our intelligent user interface that makes the
Optima X Series a breeze to operate. Our entry-level model, the XE delivers all of the
features required to quickly set up and complete a run. Our premium model, the XPN,
boasts networking capability and a variety of customizable security and tracking features,
ensuring chain of custody and compliance. Both models feature multi-layered BioSafety
features in order to maintain a safe working environment. And, as you have come to expect,
all instruments are designed as a total system with our innovative rotors and labware.
1
Large touch-screen display
Easy to read and navigate. Viewable from across the
lab. Intuitive graphical interface with on-screen help.
Multilingual software for error-free operation.
Remote monitoring and control (XPN only)
Saves time. Start, stop, and monitor from computer
or smart phone. Receive diagnostic alerts via e-mail.
Powerful on-board simulation
and calculation tools (XPN only)
Saves time and samples. Proprietary software
optimizes protocols before running to ensure
maximum efficiency. Quickly perform common
calculations and conversions.
Security and tracking features (XPN only)
Manage rotor life by serial number. Track detailed usage
history. Password protection and electronic signatures
help you maintain chain of custody and compliance.
Energy efficient
Maintains runs during power fluctuations.
Regenerative braking returns energy to local circuit.
Highly efficient thermoelectric cooling results in
lower power consumption.
Quiet-drive technology
Designed for lowest drive noise – this is the
quietest floor model on the market. Operates
at less than 51 dBA.
Part Numbers*
Model
Instrument
BioSafe Package**
Optima XPN-100
A99846
B10053
Optima XPN-80
A99839
B10051
Optima XPN-90
Optima XE-100
Optima XE-90
A99842
A99836
A99833
B10052
B10050
B10049
* Availability varies by geography. Contact your local sales representative to determine the correct part number for ordering.
**BioSafePackageincludescentrifugeandHEPAfilterkit.
1-2
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Ultracentrifugation
Features
Giant LCD touchscreen display
Intuitive, multilingual interface
Improved power tolerance
Compatible with existing Beckman Coulter rotors
Step-by-step zonal/CF operation screens
Rotor tracking by serial number
User defined programs
Remote monitoring and control
Password protection and three user access levels
Exportable run logs and electronic signatures
eXPert simulation software
Speed/temperature vs. time plot
XE
n
n
n
n
n
XPN
n
n
n
n
n
n
n
n
n
n
n
n
1
Specifications
Optima XE
Optima XPN
Maximum speed (rpm)
100,000
90,000
100,000
90,000
80,000
Maximum RCF (x g)
802,400
694,000
802,400
694,000
548,300
Speed control
± 2 rpm of set speed (above 1,000 rpm)
Set temperature
0 to 40°C in 1°C increments
Temperature control
± 0.5°C of set temperature∞
Ambient operating range
10 to 35°C
Accel / decel profiles
10 accel / 11 decel
Braking
Operating languages
Regenerative, power reclamation
English, French, Italian, German, Spanish, Japanese, Russian, Korean, and Chinese (Simplified)
Advanced software
No
User-defined programs
No
1,000 with to 30 steps each
User profiles
No
50 unique users and passwords
Electronic signature
No
Yes
Rotor tracking
No
By serial number
Data transfer
No
(3) USB 2.0 type A connectors, (1) RJ-45 connector
Heat output
3400 Btu/hr (1.0 kW)
Drive cooling
Air-cooled
Refrigeration system
Thermoelectric - no CFCs, ODCs
Sound level
Remote control
HEPA filter
Power requirement
Dimensions (H x W x D)
Weight
Optima eXPert calculations, simulations, references
<51 dBA
No
Available
Available
200-240 VAC, 30 A, 50/60 Hz
125.7 x 94 x 68.1 cm
49.5 x 37 x26.8 in
485 kg (1068 lb)
∞ ± 0.8ºC when using SW 32 swinging bucket rotor.
Beckman Coulter Ultracentrifuge
1-3
www.beckmancoultercentrifugation.com
Ultracentrifugation
Optima MAX Series Tabletop Preparative Ultracentrifuge
Elevating your laboratory’s potential starts with the optimal tabletop ultracentrifuge.
And the Optima MAX Series is the absolute top of the line. Reaching up to 150,000 rpm and
over 1,000,000 x g, the Optima MAX instruments set the standard in tabletop speed. Our
entry-level model, the MAX-TL, is designed for optimum functionality and efficiency within a
compact, quiet package. Our premium model, the Optima MAX-XP, delivers advanced software
with password-controlled security features, remote control capability, higher speed, and a wider
rotor portfolio. The Optima MAX Series takes sophistication and total system design to a whole
new level.
1
Full-color LCD touch-screen display
Easy to read and navigate. Intuitive graphical
interface with on-screen help. Multilingual
software for error-free operation.
Remote monitoring and control
(Optima MAX-XP Only)
Security and tracking features
(Optima MAX-XP Only)
Saves time. Start, stop, and monitor from
your computer. Requires Remote Control
Kit (P/N 393395), sold separately.
Manage rotor life by serial number.
Track detailed usage history. Password
protection helps you maintain chain of
custody and compliance.
Energy efficient
The optional HEPA filter combines with
innovative Beckman Coulter rotors and
labware to offer a multi-layered approach
to BioSafety.
Trusted by researchers
95% of published protocols citing tabletop or
micro-ultracentrifuges reference a Beckman
Coulter instrument or rotor. (Source: 2009
Google Scholar search)
Part Numbers*
Model
Optima MAX-XP
Optima MAX-TL
Instrument
393315
A95761
BioSafe Package**
A47882
B11229
Remote Control Package
393395
* Availability varies by geography. Contact your local sales representative to determine the correct part number for ordering.
**BioSafePackageincludescentrifugeandHEPAfilterkit.
1-4
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Features
Giant LCD touchscreen
Intuitive, multilingual interface
Numerical vacuum display
User-defined programs
TL rotor compatibility
ML rotor compatibility
Password protection and user profiles
Exportable run logs
Rotor logging by serial number
Remote monitoring and control option
RPM or RCF user selectable
On-screen rotor library
MAX-TL
n
n
n
n
n
MAX-XP
n
n
n
n
n
n
n
n
n
n
n
n
1
Specifications
MAX-TL
MAX-XP
Maximum speed (rpm)
120,000
150,000
Maximum RCF (x g)
657,000
Speed control
1,019,000
± 50 rpm at set speed
Set temperature
0 to 40°C in 1°C increments
Temperature control
± 2°C of set temperature
Ambient operating range
15 to 35°C
Accel / decel profiles
10 accel / 11 decel
Operating languages
English, French, Italian, German, Spanish, Japanese, Russian, Korean, and Chinese (Simplified)
User-defined programs
Virtually limitless
User profiles
No
Yes
Rotor tracking
No
By serial number
Data transfer
No
Heat output
Drive cooling
Air-cooled
Refrigeration system
Solid state - no CFCs, ODCs
Sound level
Remote control
HEPA filter
Power requirement
Dimensions (H x W x D)
Weight
Beckman Coulter Ultracentrifuge
USB 2.0 type A connectors
2400 Btu/hr (0.7 kW)
<47 dBA
No
Available
Available
200-240 VAC, 6 A, 50 Hz
120 VAC, 12 A, 50/60 Hz
100 VAC, 12 A, 50/60 Hz
34.5 (front left) 39.4 (rear right) x 73.9 x 61.7 cm
13.6 (front left) 15.5 (rear right) x 29.1 x 24.3 in
105 kg (230 lb)
1-5
www.beckmancoultercentrifugation.com
Ultracentrifugation
Airfuge Air-Driven Micro-Ultracentrifuge
1
The unique Airfuge Air-Driven Micro-Ultracentrifuge from
Beckman Coulter is a convenient, easy-to-use pelleting tool
in research, clinical, and industrial laboratories. Efficient and
simple to operate, the Airfuge has a line of general-purpose
and special purpose rotors for a wide variety of small-volume applications.
The easy-to-operate centrifuge:
- Reaches top speed in only 30 seconds
- Decelerates smoothly to keep sample contents separated
- Offers timed runs of up to five hours with hold position
for longer runs
- Operates near ambient temperature
Airfuge Air-Driven Micro-Ultracentrifuge with digital tachometer.
Part Numbers
340400
Airfuge
60 Hz, 120 VAC
340401
50 Hz, 220 VAC
347854
60 Hz, 120 VAC with
Digital Tachometer
347855
50 Hz, 220 VAC with
Digital Tachometer
1-6
Enhanced versatility with a variety of rotors:
- Batch rotor pellets particles from large samples
- Chylomicron Rotor achieves rapid clarification of
lipemic blood samples
- Four fixed angle rotors generate the high speeds and
forces necessary to perform a variety of tasks
Specifications
Maximum Speed
110 000 rpm
Maximum g-force (RCF x g)
199 000 x g (A 110 Rotor)
Speed Control
± 5 000 rpm of set speed
Imbalance Tolerance
Up to 10% of volume in opposite tubes
Drive Cooling
Air-cooled
Time Setting
0-300 minutes in five minute increments,
HOLD for longer runs
Accel/Decel Rates
30-60 seconds/2.5-5.5 minutes
Heat Output
0.6 kW/h (1 800 Btu/h)
Dimensions
28.0 cm (11 in) W x 38.0 cm (15 in) D
x 20.0 cm (8 in) H
Weight
10.4 kg (23 lb)
Voltage Requirements
60/50 Hz, 100 VAC
50 Hz, 220-240 VAC
60 Hz, 110 VAC
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Ultracentrifugation
Instrument Classification
Each Beckman Coulter preparative ultracentrifuge carries
a safety classification that defines which Beckman Coulter
rotors may be safely operated in that centrifuge. The letter
classifications (from A to T) take into account rotor energies,
size of the rotor chamber, and instrument updates. The classification is indicated on a decal near the rotor chamber or in
the About section of the instrument software (depending on
the model). If you are unsure of your ultracentrifuge’s
correct classification, please call your local Beckman Coulter
Service Engineer for assistance. Instruments currently being
manufactured are classified as S.
Before ordering a rotor, check your instrument’s
classification to make certain it will accommodate the rotor
selected. Each rotor listing on the following pages indicates
the instrument classifications in which they can be used.
Beckman Coulter rotors and ultracentrifuges are designed
and tested as complete systems, and engineered for safe,
reliable operation. We do not test Beckman Coulter rotors
in non-Beckman Coulter ultracentrifuges, nor non-Beckman
Coulter rotors in Beckman Coulter ultracentrifuges. It is not
recommended that Beckman Coulter rotors be used in other
instruments or that rotors made by other manufacturers be
used in Beckman Coulter ultracentrifuges.
Speed Reductions
When using any rotor, be sure to observe the instructions
given in the appropriate Rotor Manual. Speed limitations
lower than those given in this Product Selection Guide may
be required because of weight considerations—the weight of
a tube, a cap, or the density of the solution being centrifuged.
Other considerations, such as the precipitation of cesium salt,
require deration of the rotor as well. Use the equations of the
CsCl deration curves given in the Rotor Manual to determine the correct rotor speed. If a Rotor Manual has been
lost, contact your local Beckman Coulter office to request a
replacement.
Preparative Floor Model Rotor Designations
All fixed-angle preparative floor model ultracentrifuge rotors
are designated by the word Type, vertical-tube rotors by
the symbol VT, near-vertical by the symbol NVT, and swinging bucket rotors by the symbol SW. The symbol Ti means
the rotor is made of titanium. All other rotors are made of
aluminum.
k Factors
The k Factors shown in the rotor charts can be used to
compare the efficiency of various rotors for the material that
will be centrifuged. They are a guide to the time, t (in hours),
required to pellet a particle of known sedimentation coefficient, s (in Svedberg units): t=k/s.
Beckman Coulter Ultracentrifuge
Centrifugal Forces
The centrifugal forces given for rotors in these publications
have been rounded to three significant figures using the formula
where r is the radius in millimeters.
Tube Kits
For your ordering convenience, most Beckman Coulter
Fixed-Angle Rotors are sold as Rotor Assemblies with a
choice of Tube Kits. For rotors that accommodate OptiSeal
and Quick-Seal tubes, you can order kits with these tubes
instead of a Rotor Package with capped tubes only. Each kit
comes complete with up to 200 tubes, the appropriate number of cap assemblies or spacers, Vacuum Grease, and, in the
case of the OptiSeal and Quick-Seal Kits, a Tube Rack. For
complete ordering information, see the individual rotor listings that follow.
Use of Small Tubes
With the use of appropriate spacers and/or adapters, many
rotors can accommodate tubes smaller than the tube cavities.
When centrifuging small samples, use of these smaller tubes
increases the efficiency and speeds the process. A chart of
compatible tubes is provided with each rotor listing.
Innovative g-Max System
The g-Max system adds valuable capabilities to your
Beckman Coulter ultracentrifuge rotors. Based on a unique
approach to tube support within the rotor cavities, the system
lets you run smaller volumes in Fixed-Angle, Vertical-Tube
and Swinging Bucket Rotors with no reduction in g-force
and at lower k Factors to achieve separations in much shorter
run times.
This system uses patented Beckman Coulter Quick-Seal
bell-top tubes and floating spacers. Unlike conventional
sleeve-type adapters, the g-Max spacers “float” on top of the
tube which keeps the sample at the maximum radius of the
tube cavity.
Note: To help you locate g-Max tubes in the rotor listings,
these tubes are highlighted with bold, italic type. To make
it easy to order these tubes the first time, g-Max kits are
available which include enough spacers to completely
fill the rotor, as well as 50 Quick-Seal polyallomer tubes
and two tools (one for spacer removal and one for tube
removal). These kits are also listed.
1-7
1
www.beckmancoultercentrifugation.com
Ultracentrifugation
1
Quick-Reference Guide to Instrument Classification Rotor Compatibility
All ultracentrifuges with the following classifications are obsolete
and no longer supported with replacement parts or service from
Beckman Coulter: A, B, C, D, E. F, G, Q, and some L5/L5B
instruments classified as H. The L5B is the newest of these and was
last manufactured in 1983. Please contact your Beckman Coulter
Sales/Service organization for assistance with any of these models.
‡
1-8
P/N
Rotor
363013
355530
342184
337922
347299
337901
339160
343007
347261
325620
Type 100 Ti
Type 90 Ti
Type 70.1 Ti
Type 70 Ti
Type 50.4 Ti
Type 50.2 Ti
Type 45 Ti
Type 42.2 Ti
Type 25
Type 19
365898
362752
361073
362755
NVT 100
NVT 90
NVT 65.2
NVT 65
362751
362754
362759
362758
VTi 90
VTi 65.2
VTi 65.1
VTi 50
335649
342194
SW 60 Ti
SW 55 Ti
331362
331302
369651
SW 41 Ti
SW 40 Ti
SW 32.1 Ti
369650
342216
342207
SW 32 Ti
SW 28.1
SW 28
350700
CF-32 Ti
969312
Ti-15
Ti-14‡
L7
R
•
L8/L8M
H
•
•
Optima
S
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
L/LE
R
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Obsolete rotor.
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Beckman Coulter Floor Model Ultracentrifuge Rotors by Use
1
Fastest Rate-Zonal Separation
Largest Volume for Pelleting
Separation
of Virus and
Largest Volume Rate-Zonal Separation
Viral Particles
n
n
n
n
n
n
n
n
Fastest Separation
Separation of
Lipoproteins
Fastest Differential Flotation
n
n
Largest Volume
n
Largest Number of Samples
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Fastest Separation
n
n
n
n
n
n
n
n
n
Largest Volume
Beckman Coulter Ultracentrifuge
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Ti-15
VTi 50
VTi 65.2
VTi 65.1
NVT 65.2
NVT 90
NVT 65
VTi 90
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Fastest Separation
n
n
n
Fastest Density Gradient Separation
Greatest Interband Distance
n
n
n
n
n
n
Greatest Interband Space
Isopycnic
Separation of
Plasmid DNA
n
n
n
n
n
n
n
n
Pelleting RNA
Through a
Largest Volume
CsCl
Gradient
NVT 100
SW 28
n
n
Largest Number for Differential Flotation
Largest Volume for Differential Flotation
n
n
n
n
Greatest Interband Distance
n
SW 28.1
n
n
Fastest Rate-Zonal Separation
Rate-Zonal
Separation
of Proteins
in Sucrose
Gradient
SW 32 Ti
SW 32.1 Ti
SW 40 Ti
SW 41 Ti
SW 55 Ti
SW 60 Ti
n
Largest Volume Rate-Zonal Separation
CF-32 Ti
n
Type 19
n
Type 25
n
Type 45 Ti
n
Type 42.2 Ti
Type 50.2 Ti
n
Type 50.4 Ti
Type 70 Ti
Largest Volume for Pelleting
Type 70.1 Ti
Separation of
Subcellular
Particles
Type 90 Ti
Type 100 Ti
Floor Model Rotor Selection by Application
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
1-9
n
www.beckmancoultercentrifugation.com
Type 100 Ti
Fixed-Angle Rotor, Titanium
Type 100 Ti
For use in instruments classified: R S
Major applications: Rapid separation of plasmid DNA and rapid
differential centrifugation of small particles.
Axis of Rotation
rmin (39.5 mm)
rav (55.5 mm)
1
Type 100 Ti
8 x 6.0 mL
rmax (71.6 mm)
Max. RPM
Max. g
k Factor
100 000
802 000
15
Number of Tubes
Volume/Size
Rotor Capacity
8 x 6 mL
⁄2 x 2 1⁄2 in
13 x 64 mm
1
26°
48 mL
No. 363013. Type 100 Ti Rotor Assembly with 3 each 876089 O-rings,
3 each 839347 O-rings, and 2 each 363304 Overspeed Disks.
No. 363963. Quick-Seal Tube Kit with 4 boxes 344619 Polyallomer
Tubes, 8 Spacers 365470, 1 each Tube Removal Tool 361668, Spinkote
Lubricant 306812, and Vacuum Grease 335148. (Cordless
Tube Topper and Tube Rack required.)
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (pkg of 8)
Required
Adapters (qty. 1)
g-Force
k Factor
2.0
2.0
3.5
3.5
5.1
5.1
6.0
345829
363962§
349621
363961§
362248
363960§
344619
50
50
50
50
50
50
50
13 x 25
13 x 25
13 x 32
13 x 32
13 x 51
13 x 51
13 x 64
360270
360270
360270
360270
360270
360270
365470
—–—
—–—
—–—
—–—
—–—
—–—
—–—
802 000
802 000
802 000
802 000
802 000
802 000
802 000
7.0
7.0
9.4
9.4
13.5
13.5
15.0
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,
50 tubes and required tools.
Max.
Speed
100 000
100 000
100 000
100 000
100 000
100 000
100 000
Rotor Replacement Parts
363037
363039
363304
839347
876089
Rotor Lid
Rotor Handle
Overspeed Disk, 100 000 rpm
O-ring for Rotor Handle
O-ring for Rotor Lid
Spacers (Package of 8)
365470 fits 13 x 64 mm
360270 fits 13 x 51 mm
fits 13 x 32 mm
fits 13 x 25 mm
Adapters/Spacers/Caps
360270
1-10
365470
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Type 90 Ti
Type 90 Ti
8 x 13.5 mL
Type 90 Ti
Fixed-Angle Rotor, Titanium
For use in instruments classified: H R S
Major applications: Five-hour separation of plasmid DNA and rapid
differential centrifugation of small particles.
Axis of Rotation
rmin (34.2 mm)
rav (55.4 mm)
rmax (76.5 mm)
Max. RPM
Max. g
k Factor
90 000
694 000
25
25°
No. 355530. Type 90 Ti Rotor Assembly with 3 each 839347 O-rings,
3 each 876089 O-rings, and 2 each 355539 Overspeed Disks.
No. 348179. Quick-Seal Tube Kit with 2 boxes 342413 Polyallomer
Tubes, 2 boxes 344322 Ultra-Clear Tubes, 12 Spacers 342695, 1 each
348123 Tube Rack, 306812 Spinkote Lubricant, and 335148 Vacuum
Grease. (Cordless Tube Topper Kit required; see Tools, Accessories, and
Supplies.)
Number of Tubes
Volume/Size
Rotor Capacity
8 x 13.5 mL
5
⁄8 x 3 in
16 x 76 mm
108 mL
No. 348180. Open-Top Tube Kit with 2 boxes 326814 Polyallomer
Tubes, 2 boxes 344085 Ultra-Clear Tubes, 12 Tube Caps 341968, 1
Spinkote Lubricant 306812, and 1 Vacuum Grease 335148. (331202 and
305075 Tools required; see Tools, Accessories, and Supplies.)
No. 361660. OptiSeal Tube Kit with 4 boxes of OptiSeal Tubes 361623,
12 Spacers 361670 (6 pkg. of 2), 1 OptiSeal Tube Rack 361642, 1 Tube
Extraction Tool 361668, and 1 Spacer Removal Tool 338765.
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Polycarbonate
Bottle/Assembly
Thinwall
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
8.9
361623
56
16 x 60
361670§§
—–—
694 000
21
90 000
4.2
4.2
6.3
6.3
10.0
13.5
13.5
356562
357332§
345830
357334§
344622
342413
344322
50
50
50
50
50
50
50
16 x 38
16 x 38
16 x 45
16 x 45
16 x 67
16 x 76
16 x 76
345828
345828
345828
345828
344676
342695
342695
—–—
—–—
—–—
—–—
—–—
—–—
—–—
694 000
694 000
694 000
694 000
694 000
694 000
694 000
11
11
14
14
21
25
25
90 000
90 000
90 000
90 000
90 000
90 000
90 000
10.4
10.4
6.5
13.5
4.0
10.0
4.0
10.0
2.0
4.0
6.5
13.5
355603
355651∆∆
326820
326814
355644
355640
355645
355630
344091
344093
344088
344085
6
25
50
50
25
25
25
25
50
50
50
50
16 x 76
16 x 76
13 x 64
16 x 76
13 x 64
16 x 76
13 x 64
16 x 76
8 x 49
13 x 41
13 x 64
16 x 76
—–—
—–—
346256
341968
—–—
338907∆
—–—
338907∆
303624
346256
346256
341968
—–—
—–—
303313
—–—
303313
—–—
303313
—–—
303376
303402
303313
—–—
362 000
362 000
197 000
548 000
197 000
77 000
197 000
197 000
128 000
138 000
197 000
548 000
48
48
69
32
69
190
69
69
72
67
69
32
65 000
65 000
50 000
80 000
50 000∆
30 000∆
50 000∆
50 000∆
40 000
45 000
50 000
80 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
Set of 2.
∆ M
aximumspeedsgivenabovearethosewhichthetubescouldwithstandwhen
tested at 25°C for 24 hours. Further tests have shown that the polycarbonate tubes
can be run at 75 000 rpm for six hours or at 65 000 rpm for eight hours.
∆∆Bottlesonly.
§§
k Factor
Max.
Speed
Rotor Replacement Parts
355528
355529
355530
355539
356959
839347
876089
Lid
Rotor Handle
Rotor Assembly
Overspeed Disk
Tool, Handle
O Ring, Inner
O Ring, Outer
Adapters/Spacers/Caps
303313 303376
303402
303624
Beckman Coulter Ultracentrifuge
338907
341968
346256
342695
344676
345828
361670
1-11
1
www.beckmancoultercentrifugation.com
Type 70.1 Ti
Fixed-Angle Rotor, Titanium
Type 70.1 Ti
For use in instruments classified: H R S
Major applications: Differential centrifugation of subcellular particles.
Axis of Rotation
rmin (40.5 mm)
1
Type 70.1 Ti
12 x 13.5 mL
rav (61.2 mm)
rmax (82.0 mm)
Max. RPM
Max. g
k Factor
70 000
450 000
36
Number of Tubes
Volume/Size
Rotor Capacity
12 x 13.5 mL
5
⁄8 x 3 in
16 x 76 mm
24°
162 mL
No. 348180. Open-Top Tube Kit with 2 boxes 326814 Polyallomer Tubes,
2 boxes 344085 Ultra-Clear Tubes, 12 Tube Caps 341968, 1 Spinkote
Lubricant 306812, and 1 Vacuum Grease 335148. (331202 and 305075
Tools required; see Tools, Accessories, and Supplies.)
No. 361660. OptiSeal Tube Kit with 4 boxes of OptiSeal Tubes 361623,
12 Spacers 361670 (6 pkg of 2), 1 OptiSeal Tube Rack 361642, 1 Tube
Extraction Tool 361668, and 1 Spacer Removal Tool 338765.
No. 342184. Type 70.1 Ti Rotor Assembly with 3 each 011757 O-rings,
3 each 858125 O-rings, and 2 each 335585 Overspeed Disks.
No. 348179. Quick-Seal Tube Kit with 2 boxes 342413 Polyallomer
Tubes, 2 boxes 344322 Ultra-Clear Tubes, 12 Spacers 342695, 1 each
348123 Tube Rack, Spinkote Lubricant 306812, and Vacuum Grease
335148. (Cordless Tube Topper Kit required; see Tools, Accessories,
and Supplies.)
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Polycarbonate
Bottle Assembly
Thinwall
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
8.9
361623
56
16 x 60
361670§§
—–—
450 000
32
70 000
4.2
4.2
6.3
6.3
10.0
13.5
13.5
356562
357332§
345830
357334§
344622
342413
344322
50
50
50
50
50
50
50
16 x 38
16 x 38
16 x 45
16 x 45
16 x 67
16 x 76
16 x 76
345828
345828
345828
345828
344676
342695
342695
—–—
—–—
—–—
—–—
—–—
—–—
—–—
450 000
450 000
450 000
450 000
450 000
450 000
450 000
17
17
24
24
34
36
36
70 000
70 000
70 000
70 000
70 000
70 000
70 000
10.4
10.4
6.5
13.5
4.0
10.0
4.0
8.0
2.0
4.0
6.5
13.5
355603
355651∆∆
326820
326814
355644
355640
355645
355630
344091
344093
344088
344085
6
6
50
50
25
25
25
25
50
50
50
50
16 x 76
16 x 76
13 x 64
16 x 76
13 x 64
16 x 76
13 x 64
16 x 76
8 x 49
13 x 41
13 x 64
16 x 76
—–—
—–—
346256
341968
—–—
338907∆
—–—
338907∆
303624
346256
346256
341968
—–—
—–—
303313
—–—
303313
—–—
303313
—–—
303376
303402
303313
—–—
388 000
388 000
212 000
450 000
212 000
82 700
212 000
230 000
138 000
152 000
212 000
450 000
42
42
60
36
60
199
60
71
63
58
60
36
65 000
65 000
50 000
70 000
50 000∆
30 000∆
50 000∆
50 000∆
45 000
45 000
50 000
70 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
Max.
Speed
Rotor Replacement Parts
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
Set of 2.
∆ M
aximumspeedsgivenabovearethosewhichthetubescouldwithstandwhen
tested at 25°C for 24 hours. Further tests have shown that the polycarbonate tubes
can be run at 75 000 rpm for six hours or at 65 000 rpm for eight hours.
∆∆Bottlesonly.
§§
k Factor
011757
335585
337924
342183
858125
O-ring for Rotor Handle
Overspeed Disk, 70 000 rpm
Rotor Handle
Rotor Lid
O-ring for Rotor Lid
Adapters/Spacers/Caps
303313
1-12
303376
303402
303624
341968
342695
344676
345828
346256
361670
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Type 70 Ti
Type 70 Ti
8 x 39 mL
Type 70 Ti
Fixed-Angle Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
Major applications: Differential centrifugation of subcellular fractions.
rmin (39.5 mm)
rav (65.7 mm)
rmax (91.9 mm)
Max. RPM
Max. g
k Factor
70 000
504 000
44
23°
No. 337922. Type 70 Ti Rotor Assembly with 3 each 011757 O-rings,
3 each 870612 O-rings, and 2 each 335585 Overspeed Disks.
No. 348178. Quick-Seal Tube Kit with 2 boxes 342414 Polyallomer
Tubes, 2 boxes 344326 Ultra-Clear Tubes, 12 Spacers 342699, 1 each
348124 Tube Rack, 306812 Spinkote Lubricant, and 335148 Vacuum
Grease. (Cordless Tube Topper Kit required; see Tools, Accessories, and
Supplies.)
Number of Tubes
Volume/Size
Rotor Capacity
8 x 39 mL
1 x 3 1⁄2 in
25 x 89 mm
312 mL
No. 348172. Open-Top Tube Kit with 4 boxes 344367 Polyallomer
Tubes, 10 Tube Caps 337927, 1 Spinkote Lubricant 306812, and
1 Vacuum Grease 335148. (338841 and 305075 Tools required; see
Tools, Accessories, and Supplies.)
No. 361662. OptiSeal Tube Kit with 4 boxes of OptiSeal Tubes 361625,
12 Spacers 361669 (6 pkg of 2), 1 OptiSeal Tube Rack 361646,
1 Tube Extraction Tool 361668, and 1 Spacer Removal Tool 338765.
Tubes and Bottles
Tube Style/Material
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
32.4
15.0
15.0
27.0
27.0
33.0
39.0
15.0
27.0
39.0
26.3
361625
343664
357343§
343665
357346§
344623
342414
344324
344323
344326
355618
56
50
50
50
50
50
50
50
50
50
6
25 x 77
25 x 38
25 x 38
25 x 64
25 x 64
25 x 83
25 x 89
25 x 38
25 x 64
25 x 89
25 x 89
361669§§
343448
343448
343448
343448
344635
342699
343448
343448
342699
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
504 000
504 000
504 000
504 000
504 000
504 000
504 000
504 000
504 000
504 000
371 000
44
24
24
31
31
38
44
24
31
44
59
70 000
70 000
70 000
70 000
70 000
70 000
70 000
70 000
70 000
70 000
60 000
6.5
13.5
35.5
38.5
4.0
10.0
30.0
4.0
10.0
30.0
6.5
13.5
38.5
38.5
326820
326814
344367
326823
355644
355640
355642
355645
355630
355631
344088
344085
344058
301112
50
50
50
50
25
25
25
25
25
25
50
50
50
1
13 x 64
16 x 76
25 x 83
25 x 89
13 x 64
16 x 76
25 x 89
13 x 64
16 x 76
25 x 89
13 x 64
16 x 76
25 x 89
25 x 89
346256
330860
337927
331151
—–—
338907†
338906†
—–—
338907†
338906†
346256
330860
331151
302133
303392
303307
—–—
—–—
303392
303307
—–—
303392
303307
—–—
303392
303307
—–—
—–—
102 000
151 000
504 000
371 000
102 000
151 000
371 000
102 000
151 000
371 000
102 000
151 000
371 000
165 000
69
104
43
59
69
104
59
69
104
59
69
104
59
135
45 000
40 000
70 000
60 000
45 000
40 000
60 000
45 000
40 000
60 000
45 000
40 000
60 000
40 000††
OptiSeal Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Polycarbonate
Bottle Assembly
Thinwall
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Stainless Steel
k Factor
Max.
Speed
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
Set of 2.
† C
apsareoptionalfortheseThickwalltubes.IntheType70Ti,thefillinglevelfor
355642and355631is16.5mL.For355640and355630,thefillinglevelis7.5
mL. Maximum speeds for these open-top tubes without a cap: Polycarbonate—45
000 rpm. Polyallomer—20 000 rpm. These speeds are those which the tubes could
withstand when tested at 25°C for 24 hours. Further tests have shown that the
polycarbonate tube can run at 50 000 rpm for four hours.
†† For solution densities of 2.0 g/mL and below. For solution densities above 2.0 g/
mL, further speed reductions are required. Refer to publication L5-TB-072 when
centrifuging stainless steel tubes.
§§
Rotor Replacement Parts
011757
335585
337923
337924
870612
O-ring for Rotor Handle
Overspeed Disk, 70 000 rpm
Rotor Lid
Rotor Handle
O-ring for Rotor Lid
Adapters/Spacers/Caps
303307 303392
330860
331151
Beckman Coulter Ultracentrifuge
337927
338906
338907
342699
343448
344635
346256
361669
302133
1-13
1
www.beckmancoultercentrifugation.com
Type 50.4 Ti
Type 50.4 Ti
Fixed-Angle Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
Major applications: Differential flotation of lipoproteins, multisample
pelleting, or gradient separations.
rmin (80.8 mm)
rav (96.1 mm)
rmax (111.5 mm)
1
Type 50.4 Ti
44 x 6.5 mL
Max. RPM
Max. g
k Factor
50 000
270 000
312 000
39
33
Number of Tubes
Volume/Size
44 x 6.5 mL
1
⁄2 x 21⁄2 in
13 x 64 mm
20°
No. 347299. Type 50.4 Ti Rotor Assembly with 3 each 870138 O-rings,
3 each 854519 O-rings, and 2 each 330336 Overspeed Disks.
No. 356957. Quick-Seal Tube Kit with 2 boxes 344619 Polyallomer
Tubes, 2 boxes 344320 Ultra-Clear Tubes, 48 Spacers 344389, 1 Tube
Rack 348122, 1 Spacer Removal Tool 338765, 1 Spinkote Lubricant
306812, and 1 Vacuum Grease 335148. (Cordless Tube Topper Kit
required; see Tools, Accessories, and Supplies.)
No. 356956. Open-Top Tube Kit for lipoprotein flotation with 4 boxes
326820 Polyallomer Tubes, 48 Tube Caps with short stems 303113,
1 Spinkote Lubricant 306812, and 1 Vacuum Grease 335148. (301875,
305075, and 841883 Tools required.)
Rotor Capacity
286 mL
No. 356958. Open-Top Tube Kit with 2 boxes 326820 Polyallomer
Tubes, 2 boxes 344088 Ultra-Clear Tubes, 48 Tube Caps with fill holes
346256, 1 Spinkote Lubricant 306812, and 1 Vacuum Grease 335148.
(301875, 305075, and 841883 Tools required.)
No. 361659. OptiSeal Tube Kit with 4 boxes of OptiSeal Tubes 361621,
8 Spacers 361676 (4 pkg of 2), 1 OptiSeal Tube Rack 361638,
1 Tube Extraction Tool 361668, and 1 Spacer Removal Tool 338765.
Tubes and Bottles
Tube Style/Material
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
4.7
361621
56
13 x 48
361676§§
—–—
312 000
33
50 000
2.0
2.0
6.0
6.0
345829
357329§
344619
344320
50
50
50
50
13 x 25
13 x 25
13 x 64
13 x 64
345827
345827
344389
344389
—–—
—–—
—–—
—–—
312 000
312 000
312 000
312 000
15
15
33
33
50 000
50 000
50 000
50 000
6.5
326820
50
13 x 64
346256
—–—
312 000
33
50 000
4.0
355644
25
13 x 64
—–—
—–—
112 400
91
30 000
1.0
4.0
2.0
6.5
355657
355645
344091
344088
25
25
50
50
8 x 51
13 x 64
8 x 49
13 x 64
—–—
—–—
303658
346256
303823
—–—
303823
—–—
252 900
312 000
252 900
312 000
40
33
40
33
45 000
50 000
45 000
50 000
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Thinwall
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-Max Kitincludesenoughspacerstofillrotor,50tubes
and required tools.
§§
Set of 2.
k Factor
Max.
Speed
Rotor Replacement Parts
330336
337904
347298
854519
870138
Overspeed Disk, 50 000 rpm
Break-away Handle Assembly
Rotor Lid
O-ring for Rotor Handle
O-ring for Rotor Lid
Adapters/Spacers/Caps
303113
1-14
303658
303823
344389
345827
346256
361676
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Type 50.2 Ti
Type 50.2 Ti
12 x 39 mL
Type 50.2 Ti
Fixed-Angle Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
Note: This rotor cannot be used in 40 000 rpm F or H instruments or the L5-50E unless the instruments have been upgraded with a diffusion pump and vacuum indicator.
rmin (54.4 mm)
Major applications: Differential centrifugation of subcellular fractions.
rav (81.2 mm)
rmax (107.9 mm)
Max. RPM
Max. g
k Factor
50 000
302 000
69
Number of Tubes
Volume/Size
Rotor Capacity
12 x 39 mL
468 mL
1 x 31⁄2 in
24°
25 x 89 mm
No. 337901. Type 50.2 Ti Rotor Assembly with 3 each 834301 O-rings,
3 each 854519 O-rings, and 2 each 330336 Overspeed Disks.
No. 348178. Quick-Seal Tube Kit with 2 boxes 342414 Polyallomer
Tubes, 2 boxes 344326 Ultra-Clear Tubes, 12 Spacers 342699, 1 each
348124 Tube Rack, 306812 Spinkote Lubricant , and 335148 Vacuum
Grease. (Cordless Tube Topper Kit required; see Tools, Accessories, and
Supplies.)
No. 348177. Open-Top Tube Kit with 2 boxes 326823 Polyallomer
Tubes, 2 boxes 344058 Ultra-Clear Tubes, 12 Tube Caps 331151,
1 Spinkote Lubricant 306812, and 1 Vacuum Grease 335148. (331202
and 305075 Tools required; see Tools, Accessories, and Supplies.)
No. 361662. OptiSeal Tube Kit with 4 boxes of OptiSeal Tubes 361625,
12 Spacers 361669 (6 pkg of 2), 1 OptiSeal Tube Rack 361646,
1 Tube Extraction Tool 361668, and 1 Spacer Removal Tool 338765.
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Bottle Assembly
Polycarbonate
Thinwall
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Stainless Steel
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
32.4
361625
56
25 x 77
361669§§
—–—
302 000
69
50 000
15.0
15.0
27.0
27.0
33.0
39.0
15.0
27.0
39.0
26.3
343664
357343§
343665
357346§
344623
342414
344324
344323
344326
355618
50
50
50
50
50
50
50
50
50
6
25 x 38
25 x 38
25 x 64
25 x 64
25 x 83
25 x 89
25 x 38
25 x 64
25 x 89
25 x 89
343448
343448
343448
343448
344635
342699
343448
343448
342699
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
—–—
302 000
302 000
302 000
302 000
302 000
302 000
302 000
302 000
302 000
302 000
39
39
55
55
68
69
39
55
69
69
50 000
50 000
50 000
50 000
50 000
50 000
50 000
50 000
50 000
50 000
6.5
13.5
38.5
4.0
10.0
30.0
4.0
10.0
30.0
6.5
13.5
38.5
38.5
326820
326814
326823
355644
355640†
355642†
355645
355630†
355631†
344088
344085
344058
301112
50
50
50
25
25
25
25
25
25
50
50
50
1
13 x 64
16 x 76
25 x 89
13 x 64
16 x 76
25 x 89
13 x 64
16 x 76
25 x 89
13 x 64
16 x 76
25 x 89
25 x 89
346256
330860
331151
—–—
338907
338906
—–—
338907
338906
346256
330860
331151
302133
303392
303307
—–—
303392
303307
—–—
303392
303307
—–—
303392
303307
—–—
—–—
194 000
179 000
302 000
194 000
179 000
302 000
194 000
179 000
302 000
194 000
179 000
302 000
165 000
61
85
69
61
85
69
61
85
69
61
85
69
127
* Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
§§
Set of 2.
† C
apsareoptionalforthesethickwalltubes.IntheType50.2Ti,thefillinglevel
for P/N 355642 and P/N 355631 is 16.5 mL. For P/N 355640 and P/N 355630,
thefillinglevelis8mL.Maximumspeedsfortheseopen-toptubeswithoutacap:
Polycarbonate — 45 000 rpm; Polyallomer — 20 000 rpm. These speeds are those
which the tubes could withstand when tested at 25ºC for 24 hours. Further tests
have shown that the polycarbonate tubes can run at 50 000 rpm for four hours.
k Factor
Max.
Speed
43 000
40 000
50 000
43 000
40 000
50 000
43 000
45 000
50 000
43 000
40 000
50 000
37 000††
Rotor Replacement Parts
330336
337903
337904
834301
854519
Overspeed Disk, 50 000 rpm
Rotor Lid
Break-away Handle Assembly
Large O-ring for Rotor Lid
Small O-ring for Rotor Lid
†† Refer to the section on RUN SPEEDS in the Type 50.2 Rotor manual before using
stainless steel tubes.
Adapters/Spacers/Caps
303307 303392 330860
331151
Beckman Coulter Ultracentrifuge
338906
338907
342699
343448
344635
346256
361669
302133
1-15
1
www.beckmancoultercentrifugation.com
Type 45 Ti
Type 45 Ti
6 x 94 mL
Type 45 Ti
Fixed-Angle Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
Note: This rotor cannot be used in 40 000 rpm F or H instruments or the L5-50E unless the instruments have been upgraded with a diffusion pump and vacuum indicator.
rmin (35.9 mm)
1
rav (69.8 mm)
Major applications: Differential centrifugation of large volumes of
subcellular fractions and viruses.
rmax (103.8 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
45 000
235 000
133
6 x 94 mL
24°
Rotor Capacity
564 mL
11⁄2 x 4 in
38 x 102 mm
No. 339160. Type 45 Ti Rotor Assembly with 3 each 854519 O-rings,
3 each 878260 O-rings, and 2 each 335458 Overspeed Disks.
No. 348175. Quick-Seal Tube Kit with 4 boxes 345776 Polyallomer
Tubes, 4 boxes 345778 Ultra-Clear Tubes, 8 Spacers 342697, 1 each
348125 Tube Rack, 306812 Spinkote Lubricant, and 335148 Vacuum
Grease. (Cordless Tube Topper Kit required; see Tools, Accessories,
and Supplies.)
No. 348176. Open-Top Tube Kit with 4 boxes 345775 Polyallomer
Tubes, 4 boxes 345777 Ultra-Clear Tubes, 8 Tube Caps 330901,
1 Spinkote Lubricant 306812, and 1 Vacuum Grease 335148. (331202
and 305075 Tools required; see Tools, Accessories, and Supplies.)
Tubes and Bottles
Tube Style/Material
Vol. (mL)
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
k Factor
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Polycarbonate
Bottle/Assembly
Thinwall
Polyallomer
94.0
345776
25
38 x 102
342697
—–—
235 000
133
45 000
94.0
345778
25
38 x 102
342697
—–—
235 000
133
45 000
70.0
70.0
6.5
10.5
13.5
94.0
4.0
10.0
10.0
81.0
4.0
10.0
10.0
81.0
6.5
10.5
13.5
94.0
355655∆∆
355622§§§
326820
326822
326814
345775
355644
355640
355639
355643
355645
355630
355629
355628
344088
344087
344085
345777
6
6
50
50
50
25
25
25
25
25
25
25
25
25
50
50
50
25
38 x 102
38 x 102
13 x 64
13 x 89
16 x 76
38 x 102
13 x 64
16 x 76
13 x 89
38 x 102
13 x 64
16 x 76
13 x 89
38 x 102
13 x 64
13 x 89
16 x 76
38 x 102
355623
—–—
346256
346256
330860
330901
—–—
338907†
—–—
338905†
—–—
338907†
—–—
338905†
346256
346256
330860
330901
—–—
—–—
303449
303459
303448
—–—
303449
303448
303459
—–—
303449
303448
303459
—–—
303449
303459
303448
—–—
235 000
235 000
138 000
156 000
149 000
235 000
138 000
149 000
156 000
235 000
138 000
149 000
156 000
235 000
138 000
156 000
149 000
235 000
133
133
90
110
107
133
90
107
110
133
90
107
110
133
90
110
107
133
45 000†
45 000†
39 000
39 000
39 000
45 000
39 000
39 000
39 000
45 000
39 000
39 000
39 000
45 000
39 000
39 000
39 000
45 000
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Bottle assembly. Reduce speed to 35 000 rpm when the bottle is centrifuged less
thanfull(minimumfillvolumeis35mL).
∆∆Bottleonly.Reducespeedto35000rpmwhenthebottleiscentrifugedlessthan
full(minimumfillvolumeis35mL).
† CapsareoptionalfortheseThickwalltubes.IntheType45Tithefillinglevelfor
355643and355628is47mL.For355640and355630,thefillinglevelis8mL.
Maximum speeds for these open-top tubes without a cap: Polycarbonate — 30 000
rpm; Polyallomer — 15 000 rpm.
§§§
Max.
Speed
Rotor Replacement Parts
335458
339163
339164
854519
878260
Overspeed Disk, 45 000 rpm
Rotor Lid
Rotor Handle
O-ring, small, for Rotor Lid
O-ring, large, for Rotor Lid
Adapters/Spacers/Caps
303448 303449 303459
1-16
330860
330901
338905
338907
342697
346256
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Type 42.2 Ti
Type 42.2 Ti
72 x 230 µL
Fixed-Angle Rotor, Titanium
For use in instruments classified: H R S
Major applications: Differential flotation of lipoproteins, rapid
separation of viruses and microsomes.
rmeniscus (104 mm)
rmax (113 mm)
Axis of
Rotation
30°
Max. RPM
Max. g
42 000
223 000
k Factor
9
Number of Tubes
Volume/Size
72 x 230 µL
0.29 x 0.8 in
7 x 20 mm
Rotor Capacity
16.5 mL
No. 343007. Type 42.2 Rotor Package. Includes Rotor, 1 box
342303 Cellulose Propionate Tubes, 3 each 885028 O-rings, 2 each
330329 Overspeed Disks, 1 Vise Assembly 332688, 1 Rotor Handle
343008, 1 Forceps 878446, 1 Spinkote Lubricant 306812, and
1 Vacuum Grease 335148.
Tubes and Bottles
Tube Style/Material
Cellulose
Propionate
Thickwall
Polyallomer
Thickwall
Polycarbonate
Volume
(µL)
Part No.
Quantity
Size
(mm)
Caps/
Spacers (qty. 1)
g-Force
k Factor
Maximum
Speed
230
342303
100
7 x 20
—–—
223 000
9
42 000
230
343621
100
7 x 20
—–—
223 000
9
42 000
230
343775
100
7 x 20
—–—
223 000
9
42 000
Note: Originally this rotor was intended for small-sample lipoprotein
applications and was called the LP 42 Ti. However, it has become a
popular choice for processing a variety of microsamples, and the name has
beenchangedtotheType42.2Titoreflectitsmoregeneraluse.
Beckman Coulter Ultracentrifuge
Rotor Replacement Parts
330329
332688
343008
878446
885028
Overspeed Disk, 42 000 rpm
Vise Assembly
Rotor Handle
Forceps
O-ring for Rotor Lid
1-17
1
www.beckmancoultercentrifugation.com
Type 25
Type 25
100 x 1 mL
Type 25
Fixed-Angle Rotor, Aluminum
Axis of Rotation
For use in instruments classified: H R S
Major applications: Differential flotation of many small samples of
lipoproteins.
1
Row 1
Row 2
Row 3
rmax (132.1 mm)
rmax (116.3 mm)
rmax (100.4 mm)
25°
No. 347261. Type 25 Rotor Assembly with 3 each 011757 O-rings,
3 each 878272 O-rings, 2 each 330333 Overspeed Disks.
No. 348184. Quick-Seal Tube Kit with 4 boxes 345831 Quick-Seal
Polyallomer Tubes, 50 Spacers 345824, 1 Tube Rack 349661, 1 Spinkote
Lubricant 306812, and 1 Vacuum Grease 335148. (Cordless Tube Topper
Kit required; see Tools, Accessories, and Supplies.)
Max. RPM
Max. g
k Factor
25 000
70 300
81 400
92 500
84
71
62
Number of Tubes
Volume/Size
Rotor Capacity
100 x 1 mL
5
⁄16 x 2 in
8 x 51 mm
100 mL
No. 348635. Open-Top Tube Kit with 5 boxes 355657 Thickwall Polycarbonate Tubes, 1 Spinkote Lubricant 306812, and 1 Vacuum Grease
335148.
Tubes and Bottles
Tube Style/Material
Thickwall
Polycarbonate
Quick-Seal
Polyallomer
Vol. (mL)
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
k Factor
Max.
Speed
1.0
355657
25
8 x 51
—–—
—–—
92 500
62
25 000
1.0
345831
50
8 x 51
345824
—–—
92 500
62
25 000
Note: Dimensions and forces for the three rows of tubes in the Type 25 rotor
are as follows:
Row 1
Row 2
Row 3
rmin (mm)
force (g)
81.7
57 200
97.5
68 300
113.4
79 400
rav (mm)
force (g)
91.1
63 800
106.9
74 800
122.8
86 000
rmax (mm)
force (g)
100.4
70 300
116.3
81 400
132.1
92 500
Rotor Replacement Parts
011757
330333
335453
347266
347267
878272
O-ring for Rotor Handle
Overspeed Disk, 25 000 rpm
Overspeed Disk, 23 000 rpm
Rotor Lid
Rotor Handle
O-ring for Rotor Body
Spacers
345824
1-18
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Type 19
Type 19
6 x 250 mL
Type 19
Fixed-Angle Rotor, Aluminum
For use in instruments classified: H R S
Major applications: Differential centrifugation of large volumes of
viruses and large subcellular particles.
Axis of Rotation
rmin (34.4 mm)
rav (83.9 mm)
rmax (133.4 mm)
Max. RPM
Max. g
k Factor
19 000
53 900
951
Number of Tubes
Volume/Size
6 x 250 mL
23⁄8 x 43⁄4 in
60 x 121 mm
25°
Rotor Capacity
1500 mL
No. 325620. Type 19 Rotor Assembly with 3 each 325623 Gaskets,
3 each 801773 O-rings, and 2 each 330331 Overspeed Disks.
No. 325632. Type 19 Rotor Package. Includes Rotor, 6 each 334205
250mL Bottle and Cap Assemblies, 1 each 355627 (pkg of 6) 250mL
Bottle, 306812 Spinkote Lubricant, and 335148 Vacuum Grease.
Bottles
Tube Style/Material
Vol. (mL)
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
k Factor
Maximum
Speed
Bottle Assembly
Polyallomer
250.0
250.0
355627◊
334205
6
1
60 x 121
60 x 121
362246
—–—
—–—
—–—
53 900
53 900
951
951
19 000
19 000
*◊ Bottle without Cap.
Beckman Coulter Ultracentrifuge
Rotor Replacement Parts
325623
325624
330331
332826
335353
801773
Gasket for Rotor Handle
Rotor Handle
Overspeed Disk, 19 000 rpm
Rotor Lid
Bottle Extractor
O-ring for Rotor Body
1-19
1
www.beckmancoultercentrifugation.com
NVT 100
NVT 100
8 x 5.1 mL
NVT Near-Vertical Tube Rotor, Titanium
Axis of Rotation
For use in instruments classified: R S
rmin (48.3 mm)
Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a
reduction in rotor speed. For speed reductions for CsCl prescription, refer to rotor manual.
rav (57.6 mm)
1
Major applications: Separation of plasmid or mitochondrial DNA in CsCl
gradients in 2.5 - 4 h.
rmax (67.0 mm)
8°
No. 365898. NVT 100 Rotor Package with 12 each 342882 Rotor Plug
Gaskets, 1 each 342705 Vise Assembly, 367084 Vise Adapter Assembly,
858121 Torque Wrench, 976959 Torx Wrench Adapter, 306812 Spinkote
Lubricant, and 2 each 363304 Overspeed Disks.
Max. RPM
Max. g
k Factor
100 000
750 000
8
Number of Tubes
Volume/Size
Rotor Capacity
8 x 5.1mL
1
⁄2 x 2 in
13 x 51 mm
40.8 mL
No. 360979. Quick-Seal Tube Kit with 4 boxes 342412 Quick-Seal
Polyallomer Tubes, 8 Spacers 342883, 1 Tube Rack 348122, and 1 Tube
Extraction Tool 361668 (Cordless Tube Topper Kit required, see Tools,
Accessories, and Supplies).
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Spacers
(qty. 1)
Floating
Spacers (qty. 1)
g-Force
2.0
2.0
5.1
345829
357325§
342412
50
50
50
13 x 25
13 x 25
13 x 51
342883
342883
342883
345827
345827
—–—
750 000
750 000
750 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,
50 tubes and required tools.
k Factor
6
6
8
Max.
Speed
100 000
100 000
100 000
Rotor Replacement Parts
342882
363304
368545
368546
858121
976959
Gasket for Rotor Plug
Overspeed Disk, 100 000 rpm
Rotor Replacement Plug (single)
Rotor Replacement Plugs with Gaskets (set of 8)
Torque Wrench
Torx Wrench Adapter
Adapters/Spacers
342883
1-20
345827
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
NVT 90
NVT 90
8 x 5.1 mL
Type NVT 90
NVT Near-Vertical Tube Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a
reduction in rotor speed. For speed reductions for CsC1 prescription, refer to rotor manual.
rmin (52.4 mm)
rav (61.8 mm)
rmax (71.1 mm)
Major applications: Separation of plasmid or mitochondrial DNA in
CsC1 gradients in 2.5 – 4 h.
8°
No. 362752. NVT 90 Rotor Package with 12 each 342882 Rotor Plug
Gaskets, 1 each 342705 Vise Assembly, 858121 Torque Wrench, 976959
Torx Wrench Adapter, 306812 Spinkote Lubricant, and 2 each 355539
Overspeed Disks.
No. 360970. OptiSeal Tube Kit with 4 boxes 362185 OptiSeal Polyallomer Tubes, 8 Spacers 362198, 1 Tube Rack 361638, 1 Tube Extraction
Tool 361668, and 1 Spacer Removal Tool 338765.
Max. RPM
Max. g
k Factor
90 000
645 000
10
Number of Tubes
Volume/Size
Rotor Capacity
8 x 5.1 mL
1
⁄2 x 2 in
13 x 51 mm
40.8 mL
No. 360979. Quick-Seal Tube Kit with 4 boxes 342412 Quick-Seal
Polyallomer Tubes, 8 Spacers 342883, 1 Tube Rack 348122, and 1 Tube
Extraction Tool 361668 (Cordless Tube Topper Kit required, see Tools,
Accessories, and Supplies).
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Spacers
(qty. 1)
Floating
Spacers (qty. 1)
g-Force
4.9
362185
56
13 x 51
362198
—–—
645 000
10
90 000
2.0
2.0
5.1
5.1
345829
357325§
342412
344075
50
50
50
50
13 x 25
13 x 25
13 x 51
13 x 51
342883
342883
342883
342883
345827
345827
—–—
—–—
645 000
645 000
645 000
645 000
7
7
10
10
90 000
90 000
90 000
90 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes,
and required tools.
k Factor
Max.
Speed
Rotor Replacement Parts
342882
355539
368545
368546
858121
976959
Gasket for Rotor Plug
Overspeed Disk, 90 000 rpm
Rotor Replacement Plug (single)
Rotor Replacement Plugs with Gaskets (set of 8)
Torque Wrench
Torx Wrench Adapter
Adapters/Spacers
342883
345827
362198
Beckman Coulter Ultracentrifuge
1-21
1
www.beckmancoultercentrifugation.com
NVT 65.2
NVT Near-Vertical Tube Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
Note: Solutions up to 1.7 g/mL in density can be run in this rotor without a reduction in rotor speed.
rmin (68.8 mm)
1
NVT 65.2
16 x 5.1 mL
Major applications: Separation of plasmid, mitochondrial, or chromosomal DNA, proteoglycans, and lipoproteins.
rav (78.4 mm)
rmax (87.9 mm)
Max. RPM
Max. g
k Factor
65 000
416 000
15
8.5°
No. 361073. NVT 65.2 Rotor Package with 12 each 342882 Rotor Plug
Gaskets, 1 each 342705 Vise Assembly, 858121 Torque Wrench, 976959
Torx Wrench Adapter, 306812 Spinkote Lubricant, and 2 each 330338
Overspeed Disks.
No. 360972. OptiSeal Tube Kit with 4 boxes 362185 OptiSeal
Polyallomer Tubes, 16 Spacers 362198, 1 Tube Rack 361638, 1 Tube
Extraction Tool 361668, and 1 Spacer Removal Tool 338765.
Number of Tubes
Volume/Size
Rotor Capacity
16 x 5.1 mL
1
⁄2 x 2 in
13 x 51 mm
81.6 mL
No. 360977. Quick-Seal Tube Kit with 4 boxes 342412 Quick-Seal
Polyallomer Tubes, 16 Spacers 342883, 1 Tube Rack 348122, and 1 Tube
Extraction Tool 361668 (Cordless Tube Topper Kit required, see Tools,
Accessories, and Supplies).
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Spacers
(qty. 1)
Floating
Spacers (qty. 1)
g-Force
4.9
362185
56
13 x 51
362198
—–—
416 000
15
65 000
2.0
2.0
5.1
5.1
345829
357326§
342412
344075
50
50
50
50
13 x 25
13 x 25
13 x 51
13 x 51
342883
342883
342883
342883
345827
345827
—–—
—–—
416 000
416 000
416 000
416 000
7
7
15
15
65 000
65 000
65 000
65 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
k Factor
Max.
Speed
Rotor Replacement Parts
330338
342882
368545
368546
858121
976959
Overspeed Disk, 65 000 rpm
Gasket for Rotor Plug
Rotor Replacement Plug (single)
Rotor Replacement Plugs with Gaskets (set of 8)
Torque Wrench
Torx Wrench Adapter
Adapters/Spacers
342883
1-22
345827
362198
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
NVT 65
NVT 65
8 x 13.5 mL
Type NVT 65
NVT Near-Vertical Tube Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a
reduction in rotor speed.
rmin (59.5 mm)
rav (72.2 mm)
rmax (84.9 mm)
Major applications: Separation of plasmid or mitochondrial DNA in
CsC1 gradients.
Max. RPM
Max. g
k Factor
65 000
402 000
21
7.5°
No. 362755. NVT 65 Rotor Package with 12 each 349290 Rotor Plug
Gaskets, 1 each 342705 Vise Assembly, 858121 Torque Wrench, 976959
Torx Wrench Adapter, 306812 Spinkote Lubricant, and 2 each 330338
Overspeed Disks.
No. 360973. OptiSeal Tube Kit with 4 boxes 362181 OptiSeal Polyallomer Tubes, 8 Spacers 362202, 1 Tube Rack 360538, 1 Tube Extraction
Tool 361668, and 1 Spacer Removal Tool 338765.
Number of Tubes
Volume/Size
Rotor Capacity
8 x 13.5 mL
5
⁄8 x 3 in
16 x 76 mm
108 mL
No. 360976. Quick-Seal Tube Kit with 4 boxes 342413 Quick-Seal
Polyallomer Tubes, 8 Spacers 349289, 1 Tube Rack 348123, and 1 Tube
Extraction Tool 361668 (Cordless Tube Topper Kit required, see Tools,
Accessories, and Supplies).
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Spacers
(qty. 1)
Floating
Spacers (qty. 1)
g-Force
11.2
362181
56
16 x 70
362202
—–—
402 000
17
65 000
6.3
6.3
8.0
8.0
10.0
13.5
13.5
345830
357335§
344621
357337§
344622
342413
344322
50
50
50
50
50
50
50
16 x 45
16 x 45
16 x 58
16 x 58
16 x 67
16 x 76
16 x 76
349289
349289
349289
349289
349289
349289
349289
349900
349900
356571
356571
349901
—–—
—–—
402 000
402 000
402 000
402 000
402 000
402 000
402 000
8
8
11
11
15
21
21
65 000
65 000
65 000
65 000
65 000
65 000
65 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
k Factor
Max.
Speed
Rotor Replacement Parts
330338
349290
368545
392084
858121
976959
Overspeed Disk, 65 000 rpm
Gasket for Rotor Plug
Rotor Replacement Plug (single)
Rotor Replacement Plugs with Gaskets (set of 8)
Torque Wrench
Torx Wrench Adapter
Adapters/Spacers
349900
349901
349289
Beckman Coulter Ultracentrifuge
356571
362202
1-23
1
www.beckmancoultercentrifugation.com
VTi 90
VTi 90
8 x 5.1 mL
VTI-90
Vertical-Tube Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
Note: Solutions up to 1.7 g/mL in density can be run in this rotor without reduction in rotor speed.
rmin (57.9 mm)
rav (64.5 mm)
rmax (71.1 mm)
1
Major applications: High-speed density gradient separations.
Max. RPM
Max. g
k Factor
90 000
645 000
6
0°
No. 362751. VTi 90 Rotor Package with 12 each 342882 Rotor Plug
Gaskets, 1 each 342705 Vise Assembly, 858121 Torque Wrench, 976959
Torx Wrench Adapter, 306812 Spinkote Lubricant, and 2 each 355539
Overspeed Disks.
No. 360970. OptiSeal Tube Kit with 4 boxes 362185 OptiSeal Polyallomer Tubes, 8 Spacers 362198, 1 Tube Rack 361638, 1 Tube Extraction
Tool 361668, and 1 Spacer Removal Tool 338765.
Number of Tubes
Volume/Size
Rotor Capacity
8 x 5.1 mL
1
⁄2 x 2 in
13 x 51 mm
40.8 mL
No. 360979. Quick-Seal Tube Kit with 4 boxes 342412 Quick-Seal
Polyallomer Tubes, 8 Spacers 342883, 1 Tube Rack 348122, and 1 Tube
Extraction Tool 361668 (Cordless Tube Topper Kit required, see Tools,
Accessories, and Supplies).
Tubes and Bottles
Tube Style/Material
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Spacers
(qty. 1)
Floating
Spacers (qty. 1)
g-Force
4.9
362185
56
13 x 51
362198
———
645 000
6
90 000
2.0
2.0
5.1
5.1
357325§
345829
342412
344075
50
50
50
50
13 x 25
13 x 25
13 x 51
13 x 51
342883
342883
342883
342883
345827
345827
———
———
645 000
645 000
645 000
645 000
6
6
6
6
90 000
90 000
90 000
90 000
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
k Factor
Max.
Speed
Rotor Replacement Parts
342882
355539
368545
368546
858121
976959
Gasket for Rotor Plug
Overspeed Disk, 90 000 rpm
Rotor Replacement Plug (single)
Rotor Replacement Plugs with Gaskets (set of 8)
Torque Wrench
Torx Wrench Adapter
Adapters/Spacers
342883
1-24
345827
362198
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
VTi 65.2
VTi 65.2
16 x 5.1 mL
VTI-65.2
Vertical-Tube Rotor, Titanium
For use in instruments classified: H R S
Axis of Rotation
Note: Solutions up to 1.7 g/mL in density can be run in this rotor without reduction in rotor speed.
rmin (74.7 mm)
rav (81.3 mm)
rmax (87.9 mm)
Major applications: Isopycnic and rate-zonal centrifugation of small
particles.
Max. RPM
Max. g
k Factor
65 000
416 000
10
0°
No. 362754. VTi 65.2 Rotor Package with 12 each 342882 Rotor Plug
Gaskets, 1 each 342705 Vise Assembly, 858121 Torque Wrench, 976959
Torx Wrench Adapter, 306812 Spinkote Lubricant, and 2 each 330338
Overspeed Disks.
No. 360972. OptiSeal Tube Kit with 4 boxes 362185 OptiSeal Polyallomer Tubes, 16 Spacers 362198, 1 Tube Rack 361638, 1 Tube Extraction Tool 361668, and 1 Spacer Removal Tool 338765.
Number of Tubes
Volume/Size
Rotor Capacity
16 x 5.1 mL
1
⁄2 x 2 in
13 x 51 mm
81.6 mL
No. 360977. Quick-Seal Tube Kit with 4 boxes 342412 Quick-Seal
Polyallomer Tubes, 16 Spacers 342883, 1 Tube Rack 348122, and 1 Tube
Extraction Tool 361668 (Cordless Tube Topper Kit required, see Tools,
Accessories, and Supplies).
Tubes and Bottles
Tube Style/Material
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Spacers
(qty. 1)
Floating
Spacers (qty. 1)
g-Force
4.9
362185
56
13 x 51
362198
———
416 000
10
65 000
2.0
2.0
5.1
5.1
357326§
345829
342412
344075
50
50
50
50
13 x 25
13 x 25
13 x 51
13 x 51
342883
342883
342883
342883
345827
345827
———
———
416 000
416 000
416 000
416 000
10
10
10
10
65 000
65 000
65 000
65 000
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
k Factor
Max.
Speed
Rotor Replacement Parts
330338
342882
368545
368546
858121
976959
Overspeed Disk, 65 000 rpm
Gasket for Rotor Plug
Rotor Replacement Plug (single)
Rotor Replacement Plug Plugs with Gaskets (set of 8)
Torque Wrench
Torx Wrench Adapter
Adapters/Spacers
342883
345827
362198
Beckman Coulter Ultracentrifuge
1-25
1
www.beckmancoultercentrifugation.com
VTi 65.1
VTi 65.1
8 x 13.5 mL
VTI-65.1
Vertical-Tube Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
rmin (68.5 mm)
rav (76.7 mm)
rmax (84.9 mm)
1
Note: Solutions up to 1.7 g/mL in density can be run in this rotor without reduction in rotor speed.
Major applications: Isopycnic and rate-zonal centrifugation of small
particles.
0°
No. 362759. VTi 65.1 Rotor Package with 12 each 349290 Rotor Plug
Gaskets, 1 each 342705 Vise Assembly, 858121 Torque Wrench, 976959
Torque Wrench, 306812 Spinkote Lubricant, and 2 each 330338 Overspeed Disks.
No. 360973. OptiSeal Tube Kit with 4 boxes 362181 OptiSeal Polyallomer Tubes, 8 Spacers 362202, 1 Tube Rack 360538, 1 Tube Extraction
Tool 361668, and 1 Spacer Removal Tool 338765.
Max. RPM
Max. g
k Factor
65 000
402 000
13
Number of Tubes
Volume/Size
Rotor Capacity
8 x 13.5 mL
5
⁄8 x 3 in
16 x 76 mm
108 mL
No. 360976. Quick-Seal Tube Kit with 4 boxes 342413 Quick-Seal
Polyallomer Tubes, 8 Spacers 349289, 1 Tube Rack 348123, and 1 Tube
Extraction Tool 361668 (Cordless Tube Topper Kit required, see Tools,
Accessories, and Supplies).
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Spacers
(qty. 1)
Floating
Spacers (qty. 1)
g-Force
11.2
362181
56
16 x 70
362202
———
402 000
13
65 000
6.3
6.3
8.0
8.0
10.0
13.5
13.5
357335§
345830
344621
357337§
344622
342413
344322
50
50
50
50
50
50
50
16 x 45
16 x 45
16 x 58
16 x 58
16 x 67
16 x 76
16 x 76
349289
349289
349289
349289
349289
349289
349289
349900
349900
356571
356571
349901
———
———
402 000
402 000
402 000
402 000
402 000
402 000
402 000
13
13
13
13
13
13
13
65 000
65 000
65 000
65 000
65 000
65 000
65 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
k Factor
Max.
Speed
Rotor Replacement Parts
330338
349290
392083
392084
858121
976959
Overspeed Disk, 65 000 rpm
Gasket for Rotor Plug
Rotor Replacement Plug (single)
Rotor Plugs with Gaskets, Torx (set of 8)
Torque Wrench
Wrench Adapter for Torx Plug
Adapters/Spacers
349900
1-26
349901
349289
356571
362202
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
VTi 50
VTi 50
8 x 39 mL
VTI-50
Vertical-Tube Rotor, Titanium
Axis of Rotation
For use in instruments classified: H R S
rmin (60.8 mm)
rav (73.7 mm)
rmax (86.6 mm)
Note: Solutions up to 1.7 g/mL in density can be run in this rotor without reduction in rotor speed.
Major applications: Isopycnic and rate-zonal centrifugation.
0°
No. 362758. VTi 50 Rotor Package with 12 each 340825 Rotor Plug
Gaskets, 1 each 332688 Vise Assembly, 369791 Torque Wrench, 355588
Hex Plug Adapter, 306812 Spinkote Lubricant, and 2 each 330336 Overspeed Disks.
No. 360974. OptiSeal Tube Kit with 4 boxes 362183 OptiSeal Polyallomer Tubes, 10 Spacers 362204, 1 Tube Rack 360542, and 1 Tube
Extraction Tool 361668.
Max. RPM
Max. g
k Factor
50 000
242 000
36
Number of Tubes
Volume/Size
Rotor Capacity
8 x 39 mL
1 x 31⁄2 in
25 x 89 mm
312 mL
No. 360975. Quick-Seal Tube Kit with 4 boxes 342414 Quick-Seal
Polyallomer Tubes, 10 Spacers 342417, 1 Tube Rack 348124, and 1 Tube
Extraction Tool 361668 (Cordless Tube Topper Kit required, see Tools,
Accessories, and Supplies).
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Spacers
(qty. 1)
Floating
Spacers (qty. 1)
g-Force
36.2
362183
56
25 x 87
362204
———
242 000
36
50 000
15.0
15.0
27.0
39.0
15.0
27.0
39.0
357344§
343664
343665
342414
344324
344323
344326
50
50
50
50
50
50
50
25 x 38
25 x 38
25 x 64
25 x 89
25 x 38
25 x 64
25 x 89
342417
342417
342417
342417
342417
342417
342417
343448
343448◊◊
343448
———
343448◊◊
343448
———
242 000
242 000
242 000
242 000
36
36
36
36
50 000
50 000
50 000
50 000
242 000
242 000
242 000
36
36
36
50 000
50 000
50 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
◊◊ Two required.
k Factor
Max.
Speed
Rotor Replacement Parts
330336 Overspeed Disk, 50 000 rpm
340825 Gasket for Rotor Plug
355587 Rotor Plug, Hex Style (If replacing all rotor plugs,
use Hex Style)
355588 Hex Plug-wrench Adapter
369791 Torque Wrench
Adapters/Spacers
342417
343448
Beckman Coulter Ultracentrifuge
362204
1-27
1
www.beckmancoultercentrifugation.com
SW 60 Ti SW 60 Ti
Swinging-Bucket Rotor, Titanium Head, and Buckets
Axis of Rotation
For use in instruments classified: H R S
Major applications: Rate-zonal centrifugation of proteins and RNA,
differential centrifugation of subcellular fractions.
rmax (120.3 mm)
rav (91.7 mm)
rmin (63.1 mm)
1
SW 60 Ti
6 x 4 mL
Max. RPM
Max. g
k Factor
60 000
485 000
45
90°
Number of Tubes
Volume/Size
Rotor Capacity
6 x 4 mL
7
⁄16 x 23⁄8 in
11 x 60 mm
24 mL
No. 335650. SW 60 Ti Rotor Package. Includes Rotor, 2 boxes 328874
Polyallomer Tubes, 2 boxes 344062 Ultra-Clear Tubes, 1 each 331313
Bucket Holder Rack, 306812 Spinkote Lubricant, 335148 Vacuum
Grease, 331155 Overspeed Disk, and 24 each 868638 O-rings.
No. 335649. SW 60 Ti Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Quick-Seal
konical
Polyallomer
Thinwall Polyallomer
Thinwall konical
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 6)
g-Force
1.5
1.5
2.0
2.0
1.3
3.0
344624
357323§
344625
357324§
358655
358648
50
50
50
50
50
50
11 x 25
11 x 25
11 x 32
11 x 32
11 x 35
11 x 60
344674
344674
344674
344674
344674
344674
———
———
———
———
358152
358152
485 000
485 000
485 000
485 000
480 000
480 000
24
24
29
29
31
45
60 000
60 000
60 000
60 000
60 000
60 000
4.0
1.5
3.0
3.0
328874
358117
358118
355636
50
50
50
25
11 x 60
11 x 35
11 x 60
11 x 60
———
———
———
———
———
358152
358152
———
485 000
480 000
480 000
485 000
45
31
45
45
60 000
60 000
60 000
60 000
3.0
355635
25
11 x 60
———
———
485 000
45
60 000
4.0
344062
50
11 x 60
———
———
485 000
45
60 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes
and required tools.
k Factor
Max.
Speed
Rotor Replacement Parts
331155
331313
332400
335645
337943
Overspeed Disk, 60 000 rpm
Bucket Holder Rack
Rotor Stand
Bucket Cap (each)
Bucket Assembly, Titanium, with Caps and O-rings,
matched set of 6
868638 O-ring for Bucket
Adapters/Spacers
344674
1-28
358152
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
SW 55 Ti
SW 55 Ti
6 x 5 mL
SW 50 Ti
Swinging-Bucket Rotor, Titanium Head, and Buckets
(red buckets)
Axis of Rotation
For use in instruments classified: H R S
Major applications: Rate-zonal separations of small particles.
rmax (108.5 mm)
rav (84.6 mm)
rmin (60.8 mm)
No. 342196. SW 55 Ti Rotor Package. Includes Rotor, 2 boxes 326819
Polyallomer Tubes, 2 boxes 344057 Ultra-Clear Tubes, 1 each 330070
Hinge Pin Tool, 331313 Bucket Holder Rack, 306812 Spinkote Lubricant, 335148 Vacuum Grease, 328896 Overspeed Disk, and 24 each
824412 O-rings.
No. 342194. SW 55 Ti Rotor Assembly.
Max. RPM
Max. g
k Factor
55 000
368 000
48
Number of Tubes
Volume/Size
Rotor Capacity
6 x 5 mL
1
⁄2 x 2 in
13 x 51 mm
30 mL
No. 361661. OptiSeal Tube Kit with 4 boxes 361627 OptiSeal Polyallomer Tubes, 6 Spacers 361678 (3 pkg of 2), 1 OptiSeal Tube Rack
361650, and 1 Tube Extraction Tool 361668.
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
konical
Polyallomer
Thinwall Polyallomer
Thinwall konical
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 6)
g-Force
3.3
361627
56
2.0
2.0
3.2
345829
357327§
358647
5.0
3.0
13 x 33
361678§§
———
368 000
48
55 000
50
50
50
13 x 25
13 x 25
13 x 51
355535
355535
355535
———
———
358153
368 000
368 000
368 000
29
29
48
55 000
55 000
55 000
326819
358119
50
50
13 x 51
13 x 51
———
———
———
358153
368 000
368 000
48
48
55 000
55 000
3.5
349623
25
13 x 51
———
———
368 000
48
55 000
3.5
349622
25
13 x 51
———
———
368 000
48
55 000
0.8
5.0
344090
344057
50
50
5 x 41
13 x 51
———
———
356860*
———
269 000
368 000
64
48
48 000
55 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubes,
and required tools.
§§
Set of 2.
* Adapter 305527 can be used for speed up to 25 000 rpm, set of 1.
k Factor
Max.
Speed
Rotor Replacement Parts
328896
331313
332400
342190
342199
Overspeed Disk, 55 000 rpm
Bucket Holder Rack
Rotor Stand
Bucket Cap (each)
Bucket Assembly, Titanium, with Caps and O-rings,
matched set of 6
824412 O-ring for Bucket
Adapters/Spacers
355535
356860
358153
Beckman Coulter Ultracentrifuge
361678
1-29
1
www.beckmancoultercentrifugation.com
SW 41 Ti
Swinging-Bucket Rotor, Titanium Head, and Buckets
(black buckets)
Axis of Rotation
1
SW 41 Ti
6 x 13.2 mL
SW 41 Ti
rmax (153.1 mm)
For use in instruments classified: H R S
Major applications: Rate-zonal and isopycnic centrifugation of viruses,
rate-zonal centrifugation of RNA.
rav (110.2 mm)
Max. RPM
Max. g
k Factor
41 000
288 000
124
rmin (67.4 mm)
Number of Tubes
Volume/Size
Rotor Capacity
6 x 13.2 mL
9
⁄16 x 31⁄2 in
14 x 89 mm
79.2 mL
No. 331336. SW 41 Ti Rotor Package. Includes Rotor, 2 boxes 331372
Polyallomer Tubes, 2 boxes 344059 Ultra-Clear Tubes, 1 each 330070
Hinge Pin Tool, 331313 Bucket Holder Rack, 306812 Spinkote Lubricant,
335148 Vacuum Grease, 330335 Overspeed Disk, and 24 each 331309
Gaskets for Buckets.
No. 331362. SW 41 Ti Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Quick-Seal konical
Polyallomer
Thinwall Polyallomer
Thinwall konical
Polyallomer
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
3.5
5.9
4.0
8.0
13.2
10.0
355870
355537
358650
358649
331372
358120
50
50
50
50
50
50
14 x 25
14 x 47
14 x 48
14 x 89
14 x 89
14 x 89
355534
355534
355534
355534
———
———
13.2
344059
50
14 x 89
———
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
* RecommendP/N354468ExtractorTool(konicaltubeadapters).
Adapters
(qty. 6)
g-Force
k Factor
Max.
Speed
———
———
358154*
358154*
———
358154*
288 000
288 000
288 000
288 000
288 000
284 000
27
55
57
124
124
124
41 000
41 000
41 000
41 000
41 000
41 000
———
288 000
124
41 000
Rotor Replacement Parts
330335
331309
331763
331313
332400
333790
Overspeed Disk, 41 000 rpm
Gasket for Bucket
Caps, matched set of 6
Bucket Holder Rack
Rotor Stand
Bucket Assembly, Black Titanium, with Caps and Gaskets,
matched set of 6
Adapters/Spacers
355534
1-30
358154
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
SW 40 Ti
SW 40 Ti
6 x 14 mL
SW 40 Ti
Swinging-Bucket Rotor, Titanium Head, and Buckets
(red buckets)
Axis of Rotation
rmax (158.8 mm)
For use in instruments classified: H R S
Major applications: Rate-zonal centrifugation of proteins, RNA and
subcellular particles.
rav (112.7 mm)
Max. RPM
Max. g
k Factor
40 000
285 000
137
rmin (66.7 mm)
Number of Tubes
Volume/Size
Rotor Capacity
6 x 14 mL
9
⁄16 x 33⁄4 in
14 x 95 mm
84 mL
No. 331301. SW 40 Ti Rotor Package. Includes Rotor, 2 boxes 331374
Polyallomer Tubes, 2 boxes 344060 Ultra-Clear Tubes, 1 each 330070
Hinge Pin Tool, 331313 Bucket Holder Rack, 306812 Spinkote Lubricant, 335148 Vacuum Grease, 330335 Overspeed Disk, and 24 each
331309 Gaskets for Buckets.
No. 331302. SW 40 Ti Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Quick-Seal
konical
Polyallomer
Thinwall Polyallomer
Thinwall konical
Polyallomer
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
3.5
5.9
4.0
8.0
355870
355537
358650
358649
50
50
50
50
14 x 25
14 x 47
14 x 48
14 x 89
355534
355534
355534
355534
14.0
10.0
11.0
14.0
331374
358120
358121
344060
50
50
50
50
14 x 95
14 x 89
14 x 95
14 x 95
———
———
———
———
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
* RecommendP/N354468ExtractorTool(konicaltubeadapters).
Adapters
(qty. 6)
g-Force
k Factor
Max.
Speed
———
———
358154*
358154*
285 000
285 000
285 000
285 000
35
61
57
130
40 000
40 000
40 000
40 000
———
358154*
358154*
———
285 000
285 000
285 000
285 000
137
124
137
137
40 000
40 000
40 000
40 000
Rotor Replacement Parts
330335
331309
331313
331763
332400
333789
Overspeed Disk, 40 000 rpm
Gasket for Bucket
Bucket Holder Rack
Caps, matched set of 6
Rotor Stand
Bucket Assembly, Red Titanium, with Caps and Gaskets,
matched set of 6
Adapters/Spacers
355534
358154
Beckman Coulter Ultracentrifuge
1-31
1
www.beckmancoultercentrifugation.com
SW 32.1 Ti
SW 32.1 Ti
6 x 17 mL
Swinging-Bucket Rotor and Buckets, Titanium
For use in instruments classified: H R S
Major applications: Separation of subcellular particles and viruses in
density gradients.
1
rmin (64.4 mm)
rav
rmax
(113.6 mm)
Max. RPM
Max. g
k Factor
32 000
187 000
228
Number of Tubes
Volume/Size
Rotor Capacity
6 x 17 mL
5
⁄8 x 4 in
16 x 102 mm
(162.8 mm)
102 mL
Axis of Rotation
No. 369696. SW 32.1 Ti Rotor Package. Includes Rotor, 2 boxes 337986
Polyallomer Tubes, 2 boxes 344061 Ultra-Clear Tubes, 1 each 331186
Bucket Holder Rack, 306812 Spinkote Lubricant, 335148 Vacuum
Grease, 335456 Overspeed Disk, 978354 hooked O-ring Removal Tool,
and 24 each 812715 O-rings.
No. 369651. SW 32.1 Ti Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Quick-Seal
konical Polyallomer
Thinwall Polyallomer
Thinwall konical
Polyallomer
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 6)
g-Force
k Factor
4.2
6.3
8.0
10.0
18.0
12.5
356562
345830
344621
344622
356291
358653
50
50
50
50
50
50
16 x 38
16 x 45
16 x 58
16 x 67
16 x 102
16 x 102
355579
355579
355579
355579
355579
355579
———
———
———
———
———
358155
187 000
187 000
187 000
187 000
187 000
187 000
56
79
102
133
199
205
32 000
32 000
32 000
32 000
32 000
32 000
17.0
337986
50
16 x 102
———
———
187 000
228
32 000
14.5
17.0
358123
344061
50
50
16 x 102
16 x 102
———
———
358155
———
187 000
187 000
225
228
32 000
32 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
Max.
Speed
Rotor Replacement Parts
331186
332400
335456
369645
369693
812715
Bucket Holder Rack
Rotor Stand
Overspeed Disk, 32 000 rpm
Bucket Cap (each)
Bucket Assembly, with Caps and O-rings, set of 6
O-ring for Bucket
Extra Buckets for the SW 32.1 Ti
The buckets of the SW 32 can be used with the SW 32.1 rotor head.
369692 Bucket Assembly, with Caps and O-rings, set of 6
Adapters/Spacers
355579
1-32
358155
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
SW 32 Ti
SW 32 Ti
6 x 38.5 mL
Swinging-Bucket Rotor and Buckets, Titanium
For use in instruments classified: H R S
Major applications: Separation of subcellular particles and viruses in
density gradients.
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
32 000
175 000
204
6 x 38.5 mL
1 x 3.5 in
25 x 89 mm
rmin (66.8 mm)
rav
(109.7 mm)
rmax
(152.5 mm)
Rotor Capacity
231 mL
Axis of Rotation
No. 369694. SW 32 Ti Rotor Package. Includes Rotor, 1 box 326823
Polyallomer Tubes, 2 boxes 344058 Ultra-Clear Tubes, 1 box 355631
Polycarbonate Tubes, 1 each 331186 Bucket Holder Rack, 306812
Spinkote Lubricant, 335148 Vacuum Grease, 335456 Overspeed Disk,
978354 hooked O-ring Removal Tool, and 24 each 812715 O-rings.
No. 369650. SW 32 Ti Rotor Assembly.
Tubes and Bottles
Tube Style/Material
OptiSeal Polyallomer
Quick-Seal
Polyallomer
Bell-Top
Quick-Seal
konical
Polyallomer
Bell-Top
Thinwall konical
Polyallomer
Thickwall
Polyallomer
Thinwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 6)
g-Force
k Factor
32.4
15.0
27.0
33.5
8.4
22.5
28.0
361625
343664
343665
344623
358652
358654
358651
56
50
50
50
50
50
50
25.5
31.5
31.0
358125
358126
355642
38.5
25 x 77
25 x 38
25 x 64
25 x 83
25 x 38
25 x 76
25 x 83
392833§§
355536
355536
355536
355536
355536
355536
—–—
———
———
———
358156
358156
358156
175 000
175 000
175 000
175 000
175 000
175 000
175 000
155
81
146
192
74
155
198
32 000
32 000
32 000
32 000
32 000
32 000
32 000
50
50
25
25 x 76
25 x 89
25 x 89
———
———
———
358156
358156
———
175 000
175 000
175 000
156
156
198
32 000
32 000
32 000
326823
50
25 x 89
———
———
175 000
204
32 000
31.0
355631
25
25 x 89
———
———
175 000
202
32 000
38.5
344058
50
25 x 89
———
———
175 000
204
32 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
*§§ Set of 2.
Max.
Speed
Rotor Replacement Parts
331186
332400
335456
369643
369692
812715
Bucket Holder Rack
Rotor Stand
Overspeed Disk, 32 000 rpm
Bucket Cap (each)
Bucket Assembly, with Caps and O-rings, set of 6
O-ring for Bucket
Extra Buckets for the SW 32 Ti
The buckets of the SW 32.1 can be used with the SW 32 rotor head.
369693 Bucket Assembly, with Caps and O-rings, set of 6
Adapters/Spacers
355536
358156
392833
Beckman Coulter Ultracentrifuge
1-33
1
www.beckmancoultercentrifugation.com
SW 28.1
SW 28.1
6 x 18 mL
SW 28.1
Swinging-Bucket Rotor, Aluminum Head, and
Titanium Buckets
Axis of Rotation
For use in instruments classified: H R S
Note: Zonal support band (if installed in centrifuge) must be removed before operating this rotor.
1
Major applications: Rate-zonal centrifugation of subcellular particles and
viruses, rate-zonal centrifugation of subcellular fractions.
rmax (171.3 mm)
rav (122.1 mm)
rmin (72.9 mm)
Max. RPM
Max. g
k Factor
28 000
150 000
276
Number of Tubes
Volume/Size
Rotor Capacity
6 x 17 mL
5
⁄8 x 4 in
16 x 102 mm
102 mL
No. 342214. SW 28.1 Rotor Package. Includes Rotor, 2 boxes 337986
Polyallomer Tubes, 2 boxes 344061 Ultra-Clear Tubes, 1 each 331186
Bucket Holder Rack, 306812 Spinkote Lubricant, 335148 Vacuum
Grease, 342211 Overspeed Disk, and 24 each 815472 O-rings.
No. 342216. SW 28.1 Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Quick-Seal
konical Polyallomer
Thinwall Polyallomer
Thinwall konical
Polyallomer
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 6)
g-Force
k Factor
4.2
6.3
8.0
10.0
18.0
12.5
356562
345830
344621
344622
356291
358653
50
50
50
50
50
50
16 x 38
16 x 45
16 x 58
16 x 67
16 x 102
16 x 102
355579
355579
355579
355579
355579
355579
———
———
———
———
———
358155
150 000
150 000
150 000
150 000
150 000
148 000
67
99
134
160
276
276
28 000
28 000
28 000
28 000
28 000
28 000
17.0
14.5
337986
358123
50
50
16 x 102
16 x 102
———
———
———
358155
150 000
148 000
276
276
28 000
28 000
17.0
344061
50
16 x 102
———
———
150 000
276
28 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
Max.
Speed
Rotor Replacement Parts
331186
332400
342180
342211
342212
Bucket Holder Rack
Rotor Stand
Bucket Cap (each)
Overspeed Disk, 28 000 rpm
Bucket Assembly,Titanium, with Caps and O-rings,
matched set of 6
815472 O-ring for Bucket
Extra Buckets for the SW 28.1
The large-volume buckets of the SW 28 can be used with the SW 28.1
rotor head.
342217 Bucket Assembly SW 28, Titanium, with Caps and O-rings,
matched set of 6
Adapters/Spacers
355579
1-34
358155
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
SW 28
SW 28
6 x 38.5 mL
SW 28
Swinging-Bucket Rotor, Aluminum Head, and
Titanium Buckets
Axis of Rotation
For use in instruments classified: H R S
Major applications: Differential centrifugation of subcellular fractions
and viruses.
rmax (161.0 mm)
rav (118.2 mm)
rmin (75.3 mm)
Max. RPM
Max. g
k Factor
28 000
141 000
246
Number of Tubes
Volume/Size
Rotor Capacity
6 x 38.5 mL
1 x 31⁄2 in
25 x 89 mm
231 mL
No. 342204. SW 28 Rotor Package. Includes Rotor, 2 boxes 326823
Polyallomer Tubes, 2 boxes 344058 Ultra-Clear Tubes,1 each 331186
Bucket Holder Rack, 306812 Spinkote Lubricant, 335148 Vacuum
Grease, 342211 Overspeed Disk, and 24 each 812715 O-rings.
No. 342207. SW 28 Rotor Assembly.
Tubes and Bottles
Tube Style/Material
OptiSeal Polyallomer
Quick-Seal
Polyallomer
Bell-Top
Quick-Seal
konical
Polyallomer
Bell-Top
Thinwall konical
Polyallomer
Thickwall
Polyallomer
Thinwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 6)
g-Force
k Factor
32.4
15.0
27.0
33.5
8.4
22.5
28.0
361625
343664
343665
344623
358652
358654
358651
56
50
50
50
50
50
50
25.5
31.5
31.0
358125
358126
355642
38.5
25 x 77
25 x 38
25 x 64
25 x 83
25 x 38
25 x 76
25 x 83
392833§§
355536
355536
355536
355536
355536
355536
—–—
———
———
———
358156
358156
358156
141 000
141 000
141 000
141 000
141 000
141 000
141 000
206
87
164
234
87
206
234
28 000
28 000
28 000
28 000
28 000
28 000
28 000
50
50
25
25 x 76
25 x 89
25 x 89
———
———
———
358156
358156
———
141 000
141 000
141 000
206
246
246
28 000
28 000
28 000
326823
50
25 x 89
———
———
141 000
246
28 000
31.0
355631
25
25 x 89
———
———
141 000
246
28 000
38.5
344058
50
25 x 89
———
———
141 000
246
28 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
§§
Set of 2.
Max.
Speed
Rotor Replacement Parts
331186
332400
342179
342211
342217
Bucket Holder Rack
Rotor Stand
Bucket Cap (each)
Overspeed Disk, 28 000 rpm
Bucket Assembly, Titanium, with Caps and O-rings,
matched set of 6
812715 O-ring for Bucket
Extra Buckets for the SW 28
The long slender buckets of the SW 28.1 can be used with the SW 28
rotor head.
342212 Bucket Assembly SW 28.1, Titanium, with Caps and O-rings,
matched set of 6
Adapters/Spacers
355536
358156
392833
Beckman Coulter Ultracentrifuge
1-35
1
www.beckmancoultercentrifugation.com
CF-32 Ti
CF-32 Ti
430 mL
Continuous Flow Rotor, Titanium
For use in instruments classified: H S
Major applications: Isopycnic banding of viruses.
1
Max. RPM
Max. g
k Factor
Rotor Capacity
32 000
102 000
42
430 mL
CF-32 Ti Rotor Kit
No. 350700 Basic Rotor Kit. Use with Optima X and L Series
No. 350867 CF-32 Rotor (Rotor, Lid, O-ring)
CF-32 Ti Rotor Packages (for field installation of Optima X and L Series instruments)
Includes: Basic Rotor Kit, Door Kit, Adapter Bowl Assembly, and Vacuum Pump.
A19656 CF-32 Rotor Package for Optima L Series, 60 Hz
A19655 CF-32 Rotor Package for Optima L Series, 50 Hz
B10406 CF-32 Rotor Package for Optima X Series, 60 Hz
B10463 CF-32 Rotor Package for Optima X Series, 50 Hz
CF-32 Ti Accessories
354474 Door Kit and Hardware for
Optima L/XL Series
A95570 Door Kit and Hardware for
Optima X Series
B10464 Adapter Bowl Kit for Optima X and
L Series
358922 CF-32 Aspirator Vacuum Pump, 120 V
358923 CF-32 Aspirator Vacuum Pump, 220 V
961778 Vibration Mount, Optima (each; 3 needed)
355911 Vibration Mount, L/L2/L3/L5/L8 (set of 3)
354192 Standoff, CF-32 (sold in quantity of 1)
Note: A continuous-output gradient pump able to
operate against a backpressure of 20 psi (138 kPa)
is also required. The purchased pump must be able
to provide a flow of up to 150 mL/min. (9 L/hr) and
the pump head must accept 1/8-in. (3-mm) I.D.
tubing. We recommend a Masterflex pump that can
be purchased directly from the manufacturer:
www.masterflex.com
Rotor Replacement Parts
011167
303636
328946
328949
335222
335303
335456
885203
354680
807446
841687
O-ring for Stem Assembly
Backup Washer (red fiber)
Gasket for Rotor Lid
Non-extrusion Ring
Vane for Core
Core Handle
Overspeed Disk, 32 000 rpm
Adapter Bowl O-ring
Septa Assembly
Fitting, Stainless Steel
Small O-ring in Stem Assembly
Seal Assembly Replacement Parts
008025
010177
011920
010426
020198
335233
354460
354458
853156
870030
870315
354307
335233 Flat Gasket
335296 Collar
335224 Manifold
870030 Spring
011920 O-ring
010177 O-ring
354460 Static Seal
Assembly
885203 O-ring
Adapter Bowl and Door
358921 Adapter Bowl
Optima/L8
853156 O-ring
335292 Water
Jacket
Rotor Lid
810651 O-ring
812715 O-ring
354458 Rotating Seal
008025 O-ring
011920 O-ring
010426 O-ring
Bearing Assembly†††
Transfer Tube†††
335226 Bearing
Housing
020198 O-ring
354680 Septa
Assembly
335222 Vane
841687 O-ring
335309 Stem
Holder Assembly†††
O-ring under Rotating Seal (large)
O-ring in Static Seal Assembly (medium)
O-ring in Static Seal Assembly (small)
O-ring in Rotating Seal (small)
O-ring in Bearing Base
807446 Fitting
Adapter Bowl Flat Gasket/Pad
303636 Washer
Static Seal Assembly
011167 O-ring
Rotating Seal
O-ring in Static Seal Assembly (large)
Spring in Manifold
O-ring in Manifold
CF-32 Stem Assembly/Bearing Kit: Includes a matched and
serialized set of Stem Holder Assembly and Bearing Assembly.
328949 Nonextrusion Ring
328946 Gasket
812715 O-ring
Rotor Bowl
Note: The CF-32 Ti Rotor cannot be used in Beckman Coulter ultracentrifuges
thathavebeenmodifiedwiththePrepUV-ScannerorSchlierenaccessories.
††† Available only as part of Stem Assembly/Bearing Kit, P/N 354307.
1-36
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Ti-15
Ti-15
1675 mL
Zonal Rotor, Titanium
For use in instruments classified: H S
Major applications: Rate-zonal centrifugation of subcellular particles.
Max. RPM
Max. g
Rotor Capacity
Typical Sample
Volume
Particle
Pathlength
32 000
102 000
1,675 mL
50-200 mL
7.5 cm
Note: Solutions in the pH range of 4 to 10 can be used in this Titanium rotor.
No. 969312 Type Ti-15 Titanium Zonal Rotor and Lid with Standard Core
No. 969313 Type Ti-15 Titanium Zonal Rotor and Lid with B29 Core
No. 369622 Type Ti-15 Titanium Zonal Rotor and Lid without Core
All above include 332676 Push-Pull Cap and Spare Parts/Supplies –
335148 Vacuum Grease, 306812 Spinkote Lubricant, 2 extra 335456
Overspeed Disks, and O-rings. (Seal Assembly, Mounting Hardware, and
Tool Kit also required.)
Note: A continuous-output gradient pump able to operate against a
backpressure of 20 psi (138 kPa) is also required. The purchased pump
must be able to provide a flow of up to 50 mL/min. (3 L/hr) and the pump
head must accept 1/8-in. (3-mm) I.D. tubing. We recommend a
Masterflex pump that can be purchased directly from the manufacturer:
www.masterflex.com
Replacement Parts for the Ti-15 Rotor and the Discontinued
Al-15 Rotor
328946
328949
332676
333857
335456
336821
Rotor Gasket
Non-extrusion Ring
Push-Pull Cap
Rotor Core, Noryl
815472 O-ring, top of Rotor Core
011167 O-ring for Seal Fitting
812715 O-ring, bottom of Rotor Core
332682 Cone
011519 O-ring, interior of Cap
332691 Seal Fitting
012780 O-ring, visible, on Cap
Ti-15 Overspeed Disk, 32 000 rpm
Ti-15 Overspeed Disk, 29 000 rpm
Tool Kits/Additional Parts
328917
332688
332690
333763
858532
Rotor Tool Kit
Rotor Vise
Spanner Wrench
Tubing Removal Tool
Pliers
332676 Push-Pull
Capping Mechanism
011519 O-ring
Inside Cap (Not Visible)
012780 O-ring (Visible)
Rotor Lid
332682 Cone
815472 O-ring
332691 Seal Fitting
011167 O-ring for Fitting
(Not Visible)
333857 Rotor Core
354192
961778
Standoff (qty. 3 req’d
for field installations)
Vibe Mount (qty. 3 req’d
for field installations)
812715 O-ring
B-29 Type Core for Al/Ti-15 Rotors
No. 350474. This core for introduction of sample solution and recovery
of fractions from the edge as well as the center of the zonal rotor is readily interchangeable with the standard cores. The ability to load the zonal
rotor from the edge can greatly facilitate flotation-type runs. Also isopycnic banding in cesium chloride can be economically extended toTitanium
zonal rotors with the B-19 Type core.
328949 Non-Extrusion Ring
328946 Rotor Gasket
Reograd Type Core
No. 352125. Gradient is loaded and unloaded while rotor is at rest.
Gradient reorients during acceleration and reorients upon deceleration.
Does not require zonal seal assembly or mounting hardware. The rotor is
loaded using a special cap (supplied with the core) with inlet and outlet.
The cap is replaced with the standard rotor cap for the run. This core is
particularly useful for lipoprotein subfractionation.
Beckman Coulter Ultracentrifuge
Rotor Bowl
1-37
1
www.beckmancoultercentrifugation.com
Ti-14
Ti-14
665 mL
Zonal Rotor, Titanium
DISCONTINUED 2006
For use in instruments classified: H S
Major applications: Rate-zonal centrifugation of subcellular particles,
rate-zonal flotation of lipoproteins.
1
Max. RPM
Max. g
Rotor Capacity
Typical Sample
Volume
Particle
Pathlength
48 000
172 000
665 mL
20-50 mL
5.3 cm
Note: Solutions in the pH range of 4 to 10 can be used in this Titanium rotor.
Replacement Parts for the Discontinued Ti-14 Rotor
328945
328948
332676
333856
330329
332619
332620
Rotor Gasket
Non-extrusion Ring
Push-Pull Cap
Rotor Core, Noryl
815472 O-ring, top of Rotor Core
011167 O-ring for Seal Fitting
858506 O-ring, bottom of Rotor Core
332682 Cone
332691 Seal Fitting
011519 O-ring, interior of Cap
012780 O-ring, visible, on Cap
Ti-14 Overspeed Disk, 42 000 rpm
Ti-14 Overspeed Disk, 48 000 rpm
Ti-14 Overspeed Disk, 35 000 rpm
332676 Push-Pull
Capping Mechanism
011519 O-ring
Inside Cap (Not Visible)
012780 O-ring (Visible)
Tool Kits
328917
332688
332690
333763
858532
Rotor Tool Kit
Rotor Vise
Spanner Wrench
Tubing Removal Tool
Pliers
B-29 Type Core for Ti-14 Rotors
No. 350473. This core for introduction of sample solution and recovery
of fractions from the edge as well as the center of the zonal rotor is
readily interchangeable with the standard cores. The ability to load the
zonal rotor from the edge can greatly facilitate flotation-type runs. Also,
isopycnic banding in cesium chloride can be economically extended to
Titanium zonal rotors wtih the B-29 Type Core.
Rotor Lid
332682 Cone
815472 O-ring
332691 Seal Fitting
011167 O-ring for Fitting
(Not Visible)
333856 Rotor Core
858506 O-ring
328948 Non-Extrusion Ring
328945 Rotor Gasket
Note: A continuous-output gradient pump able to operate against a
backpressure of 20 psi (138 kPa) is also required. The purchased pump
must be able to provide a flow of up to 25 mL/min. (1.5 L/hr) and the
pump head must accept 1/8-in. (3-mm) I.D. tubing. We recommend a
Masterflex pump that can be purchased directly from the manufacturer:
www.masterflex.com
1-38
Rotor Bowl
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Seal Assembly
Seal Assembly
For Zonal Rotors
For use in instruments classified: H S
1
The Seal Assembly holds the lines through which fluids are introduced
and withdrawn while the rotor is spinning at 2000 rpm. The assembly
may be used with any of the Beckman Coulter zonal rotors in any Beckman Coulter preparative ultracentrifuge.
An order of a Seal Assembly should include three part numbers:
1. Seal Assembly, 334241
2. Tool Kit, 328917
3. Appropriate Mounting Hardware
If the Seal Assembly is to be used in more than one instrument, Mounting Hardware should be ordered for each instrument. If a Z-60 Rotor
is to be run in an instrument in which the Ti-14 Rotor will be used, the
336830 Support Band Kit also should be ordered (no charge).
Important Reminder: Seal Assemblies for earlier zonal rotors cannot
be used with the new rotors. To distinguish the new Seal Assembly from
the earlier version, check the rotating and stationary seals; in the newer
assembly, both seals are contained in a single unit, in the manifold assembly (see diagram).
828628 Screw (8-32 x 5/8)
802173 Washer
328960 Bearing Cap
858505 Spring
Mounting Hardware
337890 Hardware for any L8M, L8, Optima XL
Optimas with Ti 15 rotors require each of the following parts:
354192 Standoff (quantity required: 3)
961778 Vibe Mount (quantity required: 3)
We recommend the Cole Parmer #7520-40 Pump with #7015-21 Pump
Head which can be purchased directly from the manufacturer:
www.masterflex.com
Installation Requirements
The Mounting Hardware will be installed by a Beckman Coulter
Service Representative at no extra cost. In addition to the Gradient
Pump, the following installation necessities not supplied by Beckman
Coulter are needed: a syringe (50 or 100 mL) for introducing sample,
and two hemostats or other clamping devices to shut off tubing lines
when necessary.
328954 Manifold Assembly
824412 O-ring (inner)
011167 O-ring (outer, top)
811656 O-ring
328952 Stationary Seal
328951 Rotating Rulon Seal
868638 O-ring
Replacement Parts for Seal Assembly
011167
328951
328952
332618
333743
802173
811656
819527
824412
828628
868638
858505
328954
O-ring, top of Stationary Seal
Rotating Rulon Seal, for Seal Assembly
Stationary Seal, two-piece construction
Seal Disassembly Tool
Bearing and Assembly
Washer, for Bearing Cup
O-ring, outer perimeter, Stationary Seal
O-ring, for Bearing Assembly
O-ring, center top of Stationary Seal
Screw, for Bearing Cup
O-ring, for Rotating Seal
Spring, for Seal Assembly
Manifold
819527 O-ring
328959 Bearing Housing
Tool Kits
328917
332688
332690
333763
858532
333743 Bearing and Seal
Housing Assembly
Rotor Tool Kit
Rotor Vise
Spanner Wrench
Tubing Removal Tool
Pliers
Beckman Coulter Ultracentrifuge
1-39
www.beckmancoultercentrifugation.com
Ultracentrifugation
1
Quick-Reference Guide to Rotor Specifications and Tabletop Ultracentrifuge Compatibility
Max. RPM
Max. g
k Factor
No. of Tubes x Nominal
Tube Volume (mL)
Nominal Rotor
Capacity (mL)
TLA-120.2≈
120 000
627 000
16
10 x 2.0
20
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
TLA-120.1
120 000
627 000
8
14 x 0.5
7
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
TLA-110≈
110 000
657 000
13
8 x 5.1
40.8
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
TLA-100.3
100 000
541 000
14
6 x 3.5
21
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
TLA-100
100 000
436 000
7
20 x 0.2
4
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
TLA-55≈
55 000
186 000
66
12 x 1.5
18
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
TLS-55
55 000
259 000
50
4 x 2.2
8.8
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
TLN-120
120 000
585 000
7
8 x 1.2
9.6
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
TLN-100
100 000
450 000
14
8 x 3.9
31.2
TL-100, Optima TL, Optima TLX, Optima MAX-XP,
Optima MAX-TL
MLA-150
150 000
1 003 000
10.4
8 x 2.0
16
Optima MAX-XP
MLA-130
130 000
1 019 000
8.7
10 x 2.0
20
Optima MAX-XP
MLA-80
80 000
444 000
29
8x8
64
Optima MAX-XP
MLA-55
55 000
287 000
53
8 x 13.5
108
Optima MAX-XP
MLA-50
50 000
233 000
92
6 x 32.4
194.4
Optima MAX-XP
MLS-50
50 000
268 000
71
4x5
20
Optima MAX-XP
MLN-80
80 000
389 000
20
8x8
64
Optima MAX-XP
A-110
110 000
199 000
9
6 x 180 µL
1 080 µL
Airfuge
A-100/30
92 000
167 000
19
6 x 240 µL
1 440 µL
Airfuge
A-100/18
95 000
148 000
12
6 x 175 µL
1 050 µL
Airfuge
A-95
95 000
178 000
19
4 x 450 µL
1 800 µL
Airfuge
ACR-90
90 000
122 000
45
NA
3.5 mL
Airfuge
Batch Rotor
90 000
132 000
55
NA
7 mL
Airfuge
EM-90
90 000
118 000
NA
6 x 100 µL
600 µL
Airfuge
Rotor
≈
For Use in Instruments
This rotor has been tested to demonstrate containment of microbiological aerosols
only while operating within the associated Beckman Coulter centrifuge.
1-40
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Ultracentrifugation
Beckman Coulter Tabletop Rotors by Use*
n
n
n
Fastest Rate-Zonal Separation
n
n
n
n
n
n
Fastest Rate-Zonal Separation
n
n
n
Fastest Separation
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Largest Number for Differential Flotation
n
n
n
n
n
MLN-80
TLN-100
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Greatest Interband Space
n
n
n
n
n
n
n
n
n
n
n
n
Largest Volume
n
n
Largest Volume for Differential Flotation
Fastest Separation
n
n
n
n
n
Greatest Interband Distance
Isopycnic Separation
of Plasmid DNA
n
n
n
Fastest Differential Flotation
Fastest Separation
n
n
Largest Volume
Pelleting RNA Through
a CsCI Gradient
n
TLN-120
n
n
MLS-50
n
n
TLS-55
n
MLA-50
n
TLA-55
TLA-100
TLA-100.3
n
n
n
Largest Number of Samples
Separation of
Lipoproteins
n
n
Largest Volume Rate-Zonal Separation
Rate-Zonal Separation
of Proteins in Sucrose
Gradient
n
n
Largest Volume for Pelleting
Fastest Pelleting
n
MLA-55
n
Largest Volume Rate-Zonal Separation
Separation of Viruses
and Viral Particles
TLA-110
n
MLA-80
Fastest Pelleting
TLA-120.1
Largest Volume for Pelleting
TLA-120.2
Separation of
Subcellular Particles
MLA-130
Specific Application
MLA-150
Bioseparation
1
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Greatest Interband Distance
n
n
n
n
Largest Volume
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
*Selected rotor has the capability (RCF, volume, labware) to accommodate the application, but may not be the most optimal/efficient choice for the specific application.
Beckman Coulter Ultracentrifuge
1-41
www.beckmancoultercentrifugation.com
TLA-120.2≈
TLA 120.2
Fixed-Angle Rotor, Titanium
Axis of Rotation
1
TLA-120.2
10 x 2.0 mL
For use in the TL-100, Optima TL, Optima TLX, Optima MAX-XP
and Optima MAX-TL ultracentrifuges.
rmin (24.5 mm)
rav (31.8 mm)
rmax (38.9 mm)
Major applications: Pelleting of subcellular fractions in 5-30 minutes,
plasmid DNA separation in 3 hours.
30°
Max. RPM
Max. g
k Factor
120 000
627 000
16
Number of Tubes
Volume/Size
Rotor Capacity
10 x 2.0 mL
11 x 34 mm
20 mL
No. 357656. TLA-120.2 Fixed-Angle Rotor Package. Includes BioCertified Rotor, 1 box 343778 Thickwall Polycarbonate Tubes, 1 each 348305
Tube Rack, 346133 Rotor Vise/Stand, 927208 Hemostats, 824412 and
854519 O-rings, 306812 Spinkote Lubricant, and 355148 Vacuum
Grease.
No. 362046. TLA-120.2 Fixed-Angle Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Vol.(mL)
g-Max
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 1)
g-Force
1.5
2.0
1.0
344624
344625
347287
50
50
100
11 x 25
11 x 32
11 x 34
344636
344674
———
———
———
———
627 000
627 000
279 000
14
16
18
120 000
120 000
80 000
1.0
343778
100
11 x 34
———
———
627 000
8
120 000
Rotor Accessories
Rotor Replacements Parts
301875 Spacer Removal Tool
345531 Quick-Seal Starter Kit, 11 x 25 mm, 1.5 mL Tubes.
Includes 10 each 344636 Spacers, box of 344624 Tubes
and 8 each 344643 Adapters (349387 Tube Topper Rack
required).
345532 Quick-Seal Starter Kit, 11 x 32 mm, 2.0 mL Tubes.
Includes 10 each 367310 Spacers, box of 344625 Tubes
and 8 each 344644 Adapters (349387 Tube Topper Rack
required).
346133 Rotor Vise Assembly
348305 Tube Rack for 11-mm Tubes (included with Rotor Package)
349387 Tube Topper Rack for 11-mm Tubes
349487 Quick-Seal Tube Rack (Tube Rack Adapters required)√√
362100 Tube Rack Adapter (set/8) for sealing Tubes 344624*
362101 Tube Rack Adapter (set/8) for sealing Tubes 344625*
927208 Hemostats, 6-in. curved
349318
362048
824412
854519
*
k Factor
Max.
Speed
Cap & Plunger Assembly
Rotor Lid Assembly
O-ring (inner, Rotor Lid)
O-ring (outer, Rotor Lid)
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
√ √ Required for obsolete Bench Quick Sealer.
≈
This rotor has been tested to demonstrate containment of microbiological aerosols
only while operating within the associated Beckman Coulter centrifuge.
Adapters/Spacers
344636
1-42
344674
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TLA-120.1
TLA-120.1
14 x 0.5 mL
TLA 120.1
Fixed-Angle Rotor, Titanium
Axis of Rotation
For use in the TL-100, Optima TL, Optima TLX, Optima MAX-XP
and Optima MAX-TL ultracentrifuges.
Major applications: Pelleting of subcellular fractions in 5-30 minutes;
plasmid DNA separation in 3 hours.
rmin (24.5 mm)
rav (31.8 mm)
rmax (38.9 mm)
30°
Max. RPM
Max. g
120 000
627 000
k Factor
8
Number of Tubes
Volume/Size
Rotor Capacity
14 x 0.5 mL
8 x 34 mm
7 mL
No. 357655. TLA-120.1 Fixed-Angle Rotor Package. Includes Rotor,
1 box 343776 Thickwall Polycarbonate Tubes, 1 each 348304 Tube Rack,
346133 Rotor Vise/Stand, 927208 Hemostats, 824412 and 854519
O-rings, 306812 Spinkote Lubricant, and 355148 Vacuum Grease.
No. 362224. TLA-120.1 Fixed-Angle Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Thickwall
Polyallomer
Thickwall
Polycarbonate
Vol.(mL)
Part No.
Quantity
Size
(mm)
Floating
Spacers
Adapters
g-Force
0.5
343777
100
8 x 34
———
———
279 000
18
80 000
0.5
343776
100
8 x 34
———
———
627 000
8
120 000
Rotor Accessories
Rotor Replacement Parts
346133
347404
348305
927208
349318
362225
824412
854519
Rotor Vise/Stand
Rotor Cleaning Brush
Tube Rack for 8-mm Tubes (included with Rotor Package)
Hemostats
Beckman Coulter Ultracentrifuge
k Factor
Max.
Speed
Cap & Plunger Assembly
Rotor Lid Assembly (O-rings not included)
O-ring (inner, Rotor Lid)
O-ring (outer, Rotor Lid)
1-43
1
www.beckmancoultercentrifugation.com
TLA-110≈
TLA-110
8 x 5.1 mL
TLA 100.4
Fixed-Angle Rotor, Titanium
Axis of Rotation
1
For use in the TL-100, Optima TL, Optima TLX, Optima MAX-XP and
Optima MAX-TL ultracentrifuges.
Major applications: Moderate volume differential sedimentation
(pelleting) of subcellular organelles and viruses.
rmin (26.0 mm)
rav (37.2 mm)
rmax (48.5 mm)
Max. RPM
Max. g
k Factor
110 000
657 000
13
28°
Number of Tubes
Volume/Size
Rotor Capacity
8 x 5.1 mL
13 x 56 mm
40.8 mL
No. 366735. TLA-110 Fixed-Angle Rotor Package. Includes BioCertified
Rotor, 1 box of 361621 OptiSeal Tubes, 8 each 361676 Floating Spacers,
854519 and 824412 O-ring, 348122 Tube Rack, 927208 Hemostats,
306812 Spinkote Lubricant, and 335148 Vacuum Grease.
No. 366730. TLA-110 Fixed-Angle Rotor Assembly.
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Bell Top
Quick-Seal
Polyallomer
Bell Top
Thickwall
Polyallomer
Thickwall
Polycarbonate
Microcentrifuge
Polyallomer
Corning Screw Cap
Polypropylene
Vol.(mL)
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 8)
Adapters
(qty. 8)
g-Force
4.7
361621
56
13 x 48
361676§§
—–—
657 000
12
110 000
2.0
3.5
5.1
3.2
345829
349621
362248
362333
50
50
50
50
13 x 25
13 x 32
13 x 51
13 x 56
360270
360270
362307
—–—
—–—
—–—
—–—
—–—
657 000
657 000
657 000
267 000
5
7
13
37
110 000
110 000
110 000
70 000
3.2
362305
50
13 x 56
—–—
—–—
657 000
13
110 000
1.5
357448
500
11 x 38
—–—
360951
206 000
19
70 000
1.5
***
—–—
n/a
—–—
393238
536 000
9
110 000
*§§ Set of 2.
≈
This rotor has been tested to demonstrate containment of microbiological aerosols
only while operating within the associated Beckman Coulter centrifuge.
Rotor Accessories
306812 Spinkote Lubricant (2 oz)
335148 Silicone Vacuum Grease
338765 Spacer Removal Tool for 3.5-mL and 2.0-mL Quick-Seal
Tube Spacers
339555 Beckman Solution 555 (1 qt)
339558 Rotor Cleaning Kit
342025 Fraction Recovery System
345832 Tube Rack Adapter for 13 x 25 mm, 2.0-mL Quick-Seal Tubes
for use in 349487 Tube Rack√√
347404 Rotor Cleaning Brush
347828 Fraction Recovery System Adapter Kit for TL-series tubes
347960 Centri-Tube Slicer
348122 Tube Topper Rack for 13-mm Tubes
(included in Rotor Package)
348299 CentriTube Slicer Replacement Blades (pkg of 10)
349487 Tube Rack for g-Max Quick-Seal Tubes√√
354526 CentriTube Slicer Adapter for 13mm tubes
355582 Tube Rack Adapter for 13 x 32 mm, 3.5-mL Quick-Seal Tubes
for use in 349487 Tube Rack√√
k Factor
Max.
Speed
*** Corning P/N 430909. Commercially available product. Beckman Coulter does
not sell Corning tubes.
355872
358312
358313
358314
358315
361638
361668
927208
961519
Tube Rack for 13-mm Tubes
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
OptiSeal Tube Rack Assembly
Tube Removal Tool
Hemostats, 6-in. curved
Hemostat, 6 in. straight
√ √ Required for obsolete Bench Quick Sealer.
Rotor Replacement Parts
346133
347903
349477
366732
824412
854519
Rotor Vise
Spring
Cap & Plunger Assembly
Rotor Lid Assembly
O-ring (inner, Rotor Lid)
O-ring (outer, Rotor Lid)
Adapters/Spacers
360270
360951
361676
362307
393238
Adapter for Corning Screw Cap Tube
(CorningP/N430909).
1-44
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TLA-100.3
TLA-100.3
6 x 3.5 mL
TLA 100.3
Fixed-Angle Rotor, Titanium
For use in the TL-100, Optima TL, Optima TLX, Optima MAX-XP
and Optima MAX-TL ultracentrifuges.
Axis of Rotation
Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a
reduction in rotor speed.
rmin (27.5 mm)
rav (37.9 mm)
rmax (48.3 mm)
Major applications: Pelleting of subcellular fractions in 5-30 minutes;
pelleting RNA in 1-2 hours; plasmid DNA separation in 6 hours.
30°
Max. RPM
Max. g
k Factor
100 000
541 000
14
Number of Tubes
Volume/Size
Rotor Capacity
6 x 3.5 mL
13 x 51 mm
21 mL
No. 349481. TLA-100.3 Fixed-Angle Rotor Assembly.
No. 349490. TLA-100.3 Fixed-Angle Rotor Package. Includes Rotor,
1 box 349622 Thickwall Polycarbonate Tubes, 1 box 349623
Thickwall Polyallomer Tubes, 1 each 355919 (pkg. of 6) Spacers for
1.5 mL Microcentrifuge Tubes, 355872 Tube Rack, 824412 and 854521
O-rings, 927208 Hemostats, 346133 Rotor Vise/Stand, 306812 Spinkote
Lubricant, and 335148 Vacuum Grease.
Tubes and Bottles
Tube Style/Material
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 6)
Required
Adapters (qty. 6)
Quick-Seal
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Microcentrifuge
Polyallomer
2.0
3.5
3.0
345829
349621
349623
50
50
25
13 x 25
13 x 32
13 x 51
360270◊◊◊
355937
———
———
———
———
541 000
541 000
265 000
11
14
34
100 000
100 000
70 000
3.0
349622
25
13 x 51
———
———
541 000
16
100 000
1.5
357448
500
11 x 38
———
355919
245 000
24
70 000
g-Force
k Factor
Max.
Speed
Note: To help you locate g-Max tubes in the rotor listings, these tubes are
highlighted with bold, italic type.
◊◊◊ Package of 8.
Rotor Accessories
306812 Spinkote Lubricant (2 oz)
335148 Silicone Vacuum Grease
338765 Spacer Removal Tool for 3.5-mL and 2.0-mL Quick-Seal
Tube Spacers
339555 Beckman Solution 555 (1 qt)
339558 Rotor Cleaning Kit
342025 Fraction Recovery System
345832 Tube Rack Adapter for 13 x 25 mm, 2.0-mL Quick-Seal Tubes
for use in 349487 Tube Rack√√
347404 Rotor Cleaning Brush
347828 Fraction Recovery System Adapter Kit for TL-series tubes
347960 Centri-Tube Slicer
348122 Tube Topper Rack for 13-mm Tubes
348299 CentriTube Slicer Replacement Blades (pkg of 10)
349487 Tube Rack for Quick-Seal Tube Sealer, 8-place
(Adapters required)√√
354526 CentriTube Slicer Adapter for 13mm tubes
355582 Tube Rack Adapter for 13 x 32 mm, 3.5-mL Quick-Seal Tubes
for use in 349487 Tube Rack√√
355872 Tube Rack for 13-mm Tubes (included in Rotor Package)
358312 Quick-Seal Cordless Tube Topper Kit, 60 Hz
358313
358314
358315
361668
927208
961519
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
Tube Removal Tool
Hemostat, 6 in. curved
Hemostat, 6 in. straight
√ √ Required for obsolete Bench Quick Sealer.
Rotor Replacement Parts
346133
347903
349477
349478
824412
854521
Rotor Vise
Spring
Cap & Plunger Assembly
Rotor Lid Assembly
O-ring (inner, Rotor Lid)
O-ring (outer, Rotor Lid)
Adapters/Spacers
355919
355937
360270
Beckman Coulter Ultracentrifuge
1-45
1
www.beckmancoultercentrifugation.com
TLA-100
TLA-100
20 x 0.2 mL
TLA 100
Fixed-Angle Rotor, Titanium
For use in the TL-100, Optima TL, Optima TLX, Optima MAX-XP
and Optima MAX-TL ultracentrifuges.
Axis of Rotation
1
Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a
reduction in rotor speed.
rmin (30.0 mm)
rav (34.5 mm)
rmax (38.9 mm)
Major applications: Pelleting of subcellular fractions; proteins; RNA.
30°
Max. RPM
Max. g
100 000
436 000
k Factor
7
Number of Tubes
Volume/Size
Rotor Capacity
20 x 0.2 mL
7 x 20 mm
4 mL
No. 343837. TLA-100 Fixed-Angle Rotor Package. Includes Rotor,
1 box 343775 Thickwall Polycarbonate Tubes, 1 each 348302 Tube Rack,
824412 and 824953 O-rings, 927208 Hemostats, 346133 Rotor Vise/
Stand, 306812 Spinkote Lubricant, and 335148 Vacuum Grease.
No. 343840. TLA-100 Fixed-Angle Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Thickwall
Polyallomer
Thickwall
Polycarbonate
Thickwall
Cellulose Propionate
Vol.(mL)
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 1)
g-Force
0.2
343621
100
7 x 20
———
———
279 000
10
80 000
0.2
343775
100
7 x 20
———
———
436 000
7
100 000
0.2
342303
100
7 x 20
———
———
184 000
16
65 000
Rotor Accessories
Rotor Replacement Parts
306812
335148
339555
339558
342025
347404
347828
347960
348299
348302
361668
927208
961519
343845
346133
349318
824412
824953
1-46
Spinkote Lubricant (2 oz)
Silicone Vacuum Grease
Beckman Solution 555 (1 qt)
Rotor Cleaning Kit
Fraction Recovery System
Rotor Cleaning Brush
Fraction Recovery System Adapter Kit for TL-series tubes
Centri-Tube Slicer
CentriTube Slicer Replacement Blades (pkg of 10)
Tube Rack for 7-mm Tubes (included with Rotor Package)
Tube Removal Tool
Hemostats, 6-in. curved
Hemostat, 6 in. straight
k Factor
Max.
Speed
Rotor Lid Assembly (O-rings not included)
Rotor Vise
Cap & Plunger Assembly
O-ring (inner, Rotor Lid)
O-ring (outer, Rotor Lid)
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TLA-55≈
TLA-55
12 x 1.5 mL
TLA 45
Fixed-Angle Rotor, Aluminum
Axis of
Rotation
For use in the TL-100, Optima TL, Optima TLX, Optima MAX-XP and
Optima MAX-TL ultracentrifuges.
Major applications: Rapid pelleting of nucleic acid precipitates in
1.5 mL tubes.
rmin (25 mm)
rav (40 mm)
rmax (55 mm)
Max. RPM
Max. g
k Factor
55 000
186 000
66
45°
Number of Tubes
Volume/Size
Rotor Capacity
12 x 1.5 mL
11 x 38 mm
18 mL
No. 366725. TLA-55 Fixed-Angle Rotor Package. Includes BioCertified
Rotor, 1 box 357448 Microcentrifuge Tubes, 1 each 824412 and 824644
O-rings, 348122 Tube Rack, 927208 Hemostats, 306812 Spinkote Lubricant and 335148 Vacuum Grease.
Tubes and Bottles
Tube Style/Material
Vol. (mL)
Part No.
Quantity
Microcentrifuge
Polyallomer
1.5
357448
500
Size
(mm)
11 x 38
Required Caps/
Spacers (qty. 1)
———
Required
Adapters (qty. 1)
———
Rotor Accessories
Rotor Replacement Parts
347404 Rotor Cleaning Brush
348122 Tube Rack
927208 Hemostats, 6-in curved
349318
366722
824412
824644
≈
g-Force
186 000
k Factor
66
Max.
Speed
55 000
Cap & Plunger Assembly
Rotor Lid Assembly
O-ring (Inner, Rotor Lid)
O-ring (Outer, Rotor Lid)
This rotor has been tested to demonstrate containment of microbiological aerosols
only while operating within the associated Beckman Coulter centrifuge.
Beckman Coulter Ultracentrifuge
1-47
1
www.beckmancoultercentrifugation.com
MLA-150
MLA-150
8 x 2.0 mL
Fixed-Angle Rotor, Titanium
30°
For use in the Optima MAX-XP ultracentrifuge.
Major applications: Rapid differential sedimentation (pelleting) of small
particles such as subcellular organelles and viruses.
1
Max. RPM
150 000
rmin (15.8 mm)
rav (27.8 mm)
rmax (39.9 mm)
Max. g
k Factor
1 003 000
10.4*
Number of Tubes
Volume/Size
Rotor Capacity
8 x 2.0 mL
11 x 32 mm
16 mL
* Based on cell hole dimensions. k-Factor = 4.58 if run with 1 mL
sample and open top tube.
Axis of rotation
No. 393490. MLA-150 Rotor Package. Includes Rotor, 1 box 344625 Quick-Seal
Polyallomer Tubes, 1 each 393570 (pkg of 8) Spacers, 349387 Tube Rack, 860218
O-ring, 927208 Hemostats, 306812 Spinkote Lubricant, and 335148 Vacuum Grease.
No. 393489. MLA-150 Fixed-Angle Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Vol. (mL)
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 8)
Adapters
(qty. 1)
g-Force
k Factor
1.5
2.0
1.0
344624
344625
347287
50
50
100
11 x 25
11 x 32
11 x 34
393570
393570
—–—
—–—
—–—
—–—
989 000
989 000
387 000
6.2
8.0
11.4
150 000
150 000
95 000
1.0
343778
100
11 x 34
—–—
—–—
964 000
4.6
150 000
Rotor Accesories
Rotor Replacement Parts
306812
335148
339555
339558
342025
347404
347828
349387
358312
358313
358314
358315
361668
927208
961519
393485 Rotor Lid Assembly
860218 O-ring for Lid
Spinkote Lubricant (2 oz)
Silicone Vacuum Grease
Beckman Solution 555 (1 qt)
Rotor Cleaning Kit
Fraction Recovery System
Rotor Cleaning Brush
Fraction Recovery System Adapter Kit for TL-series tubes
Tube Topper Rack
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
Tube Removal Tool
Hemostat, 6 in. curved
Hemostat, 6 in. straight
Max.
Speed
Adapters/Spacers
393570
1-48
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
MLA-130
MLA-130
10 x 2.0 mL
Type 28
Fixed-Angle Rotor, Titanium
Axis of Rotation
rmin (29.9 mm)
For use in the Optima MAX-XP ultracentrifuge.
Major applications: Rapid differential sedimentation (pelleting) of small
particles such as subcellular organelles and viruses.
rav (41.9 mm)
Max. RPM
rmax (53.9 mm)
130 000
Max. g
k Factor
1 019 000
8.7
35°
Number of Tubes
Volume/Size
Rotor Capacity
10 x 2.0 mL
11 x 32 mm
20 mL
No. 367114. MLA-130 Fixed-Angle Rotor Package. Includes Rotor,
1 box 344625 Quick-Seal Polyallomer Tubes, 10 each 367310 Spacers,
1 each 349387 Tube Rack, 975001 O-ring, 927208 Hemostats, 306812
Spinkote Lubricant, and 335148 Vacuum Grease.
No. 367120. MLA-130 Fixed-Angle Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Vol. (mL)
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 1)
1.5
2.0
1.0
344624
344625
347287
50
50
100
11 x 25
11 x 32
11 x 34
367310
367310
—–—
—–—
—–—
—–—
1 019 000
1 019 000
390 000
7.0
8.7
23
130 000
130 000
80 000
1.0
343778
100
11 x 34
—–—
—–—
1 019 000
5.8
130 000
Rotor Accesories
Rotor Replacement Parts
306812
335148
339555
339558
342025
347404
347828
349387
358312
358313
358314
358315
361668
927208
961519
347373 Rotor Vise
367115 Rotor Lid
975001 O-ring for Lid
Spinkote Lubricant (2 oz)
Silicone Vacuum Grease
Beckman Solution 555 (1 qt)
Rotor Cleaning Kit
Fraction Recovery System
Rotor Cleaning Brush
Fraction Recovery System Adapter Kit for TL-series tubes
Tube Topper Rack
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
Tube Removal Tool
Hemostat, 6 in. curved
Hemostat, 6 in. straight
g-Force
k Factor
Max.
Speed
Adapters/Spacers
367310
Beckman Coulter Ultracentrifuge
1-49
1
www.beckmancoultercentrifugation.com
MLA-80
MLA-80
8 x 8 mL
Type 50 Ti
Fixed-Angle Rotor, Titanium
Axis of Rotation
For use in the Optima MAX-XP ultracentrifuge.
Major applications: Large volume differential sedimentation (pelleting)
of subcellular organelles and viruses.
rmin (29.5 mm)
rav (45.7 mm)
rmax (61.9 mm)
1
Max. RPM
Max. g
k Factor
80 000
444 000
29
Number of Tubes
Volume/Size
Rotor Capacity
8 x 8 mL
16 x 64 mm
26°
64 mL
No. 367096. MLA-80 Fixed-Angle Rotor Package. Includes Rotor,
1 box 344621 Quick-Seal Polyallomer Tubes, 10 each 367094 Spacers,
1 each 348123 Tube Rack, 927208 Hemostats, 338765 Spacer Removal
Tool, 306812 Spinkote Lubricant, and 335148 Vacuum Grease.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Vol. (mL)
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
k Factor
4.2
6.3
8.0
6.5
356562
345830
344621
355646
50
50
50
25
16 x 38
16 x 45
16 x 58
16 x 64
367094
367094
367094
———
———
———
———
444 000
444 000
444 000
84 900
18
23
29
153
80 000
80 000
80 000
35 000
6.5
355647
25
16 x 64
———
417 000
19
80 000
Rotor Accessories
Rotor Replacement Parts
306812
335148
338765
339555
339558
347404
348123
358312
358313
358314
358315
361668
927208
961519
346133 Rotor Vise
367089 Rotor Lid
892292 O-ring
Spinkote Lubricant (2 oz)
Silicone Vacuum Grease
Floating Spacer Removal Tool
Beckman Solution 555 (1 qt)
Rotor Cleaning Kit
Rotor Cleaning Brush
Tube Topper Rack
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
Tube Removal Tool
Hemostat, 6 in. curved
Hemostat, 6 in. straight
Max.
Speed
Adapters/Spacers
367094
1-50
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
MLA-55
MLA-55
8 x 13.5 mL
Fixed-Angle Rotor, Aluminum
35°
For use in the Optima MAX-XP ultracentrifuge.
Major applications: Plasmid DNA Separations.
rmin
rav
rmax
rmin (45.0 mm)
rav (64.0 mm)
rmax (84.5 mm)
Max. RPM
Max. g
k Factor
55 000
287 000
53
Number of Tubes
Volume/Size
Rotor Capacity
8 x 13.5 mL
16 x 76 mm
108 mL
Axis of Rotation
No. A31459. MLA-55 Fixed-Angle Rotor Package. Includes Rotor,
4 boxes of OptiSeal Tubes 361623, 12 spacers 361670 (6 pkg. of 2),
1 OptiSeal Tube Rack 361642, 1 Tube Extraction Tool 361668, and
1 Spacer Removal Tool 338765.
No. 393203, MLA-55 Fixed-Angle Rotor Assembly.
No. 348179. Quick-Seal Tube Kit with 2 boxes 342413 Polyallomer
Tubes, 2 boxes 344322 Ultra-Clear Tubes, 12 each 342695 Spacers,
1 each 348123 Tube Rack, 306812 Spinkote Lubricant, and 335148
Vacuum Grease. (Cordless Tube Topper Kit required; see Tools, Accessories, and Supplies.)
No. 348180. Open-Top Tube Kit with 2 boxes 326814 Polyallomer
Tubes, 2 boxes 344085 Ultra-Clear Tubes, 12 each 341968 Tube Caps, 1
each 306812 Spinkote Lubricant, and 335148 Vacuum Grease. (331202
and 305075 Tools required; see Tools, Accessories, and Supplies.)
Tubes and Bottles
Tube Style/Material
Vol. (mL)
g-Max
Part No.
Quantity
Size
(mm)
OptiSeal Bell-Top
Polycarbonate
Bottle Assembly
Quick-Seal
Polyallomer
8.9
10.4
361623
355603
56
6
16 x 60
16 x 76
4.2
4.2
6.3
6.3
10.0
13.5
13.5
356562
357332§
345830
357334§
344622
342413
344322
50
50
50
50
50
50
50
4.0
10.0
4.0
10.0
6.5
13.5
2.0
4.0
6.5
13.5
355644
355640
355645
355630
326820
326814
344091
344093
344088
344085
25
25
25
25
50
50
50
50
50
50
Quick-Seal
Ultra-Clear
Thickwall
Polyallomer
Thickwall
Polycarbonate
Thinwall
Polyallomer
Ultra-Clear
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
k Factor
Max.
Speed
361670§§
———
———
———
289 996
288 658
52
67
55 000
55 000
16 × 38
16 × 38
16 × 45
16 × 45
16 x 67
16 x 76
16 x 76
345828
345828
345828
345828
344676
342695
342695
———
———
———
———
———
———
———
289 335
289 335
289 335
289 335
289 335
289 335
289 674
28
28
37
37
60
77
74
55 000
55 000
55 000
55 000
55 000
55 000
55 000
13 × 64
16 x 76
13 × 64
16 x 76
13 x 64
16 x 76
8 x 49
13 × 41
13 x 64
16 x 76
———
338907
———
338907
346256
341968
303624
346256
346256
341968
303313
———
303313
———
303313
———
303376
303402
303313
———
214 200
85 176
214 200
286 286
215 880
289 335
143 718
145 379
215 600
291 029
52
177
52
53
56
59
80
53
57
61
50 000
30 000
50 000
55 000
50 000
55 000
40 000
45 000
50 000
55 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are highlighted with bold, italic type.
§
To simplify ordering, this g-MaxKitincludesenoughspacerstofillrotor,50tubesandrequiredtools.
§§
Set of 2.
Adapters/Spacers
303313 303376 303402
303624
Beckman Coulter Ultracentrifuge
338907
341968
346256
342695
344676
345828
361670
1-51
1
www.beckmancoultercentrifugation.com
MLA-55 (cont’d)
MLA-55
8 x 13.5 mL
1
Rotor Accessories
Rotor Replacement Parts
301875
305075
306812
331202
347373
393206
393210
839347
876089
335148
338765
339379
339555
339558
348123
358312
358313
358314
358315
361642
361668
841883
1-52
Tube Removal Tool
Tube-Cap Vise
Spinkote Lubricant (2 oz)
Tool Kit for Aluminum Caps
Includes:
858121 Torque Wrench
858122 Socket Adapter
858123 Socket for 19 mm Hex Nuts
870432 Socket for 11-mm Hex Nuts
301875 Removal Tool, for capped tubes
Silicone Vacuum Grease
Floating Spacer Removal Tool
Rotor Cleaning Brush
Beckman Solution 555 (1 qt)
Rotor Cleaning Kit
Tube Topper Rack
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
OptiSeal Tube Rack Assembly
Tube Removal Tool for Quick-Seal and OptiSeal Tubes
Hex Driver for 11-mm Cap Nuts on Stainless Steel Tubes
Rotor Vise
Rotor Handle
Rotor Lid
Handle O-Ring (Small)
Lid O-Ring (Large)
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
MLA-50
MLA-50
6 x 32.4 mL
30°
Fixed-Angle Rotor, Aluminum
For use in the Optima MAX-XP ultracentrifuge.
Major applications: Large volume separations of subcellular particles,
viruses, lipoproteins, RNA, and plasmid DNA.
rmin
rav
rmax
rmin (33.6 mm)
rav (58.4 mm)
rmax (83.2 mm)
Max. RPM
Max. g
k Factor
50 000
233 000
92
Axis of Rotation
Number of Tubes
Volume/Size
Rotor Capacity
6 x 32.4 mL
25 x 77 mm
194.4 mL
No. A91774. MLA-50 Fixed-Angle Rotor Assembly.
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
Ultra-Clear
Thinwall
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Vol. (mL)
Part No.
Quantity
Size
(mm)
32.4
361625
56
25 x 77
15.0
15.0
27.0
27.0
15.0
27.0
6.5
13.5
4.0
10.0
4.0
10.0
343664
357343 §
343665
357346 §
344324
344323
326820
326814
355644
355640
344645
344630
50
50
50
50
50
50
50
50
25
25
25
25
25 × 38
25 x 38
25 x 64
25 x 64
25 × 38
25 x 64
13 x 64
16 x 76
13 x 64
16 x 76
13 x 64
16 x 76
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
Max RCF
(x g)
k
Factor
Max. Speed
(rpm)
361669§§
———
233 000
92
50 000
343448
343448
343448
343448
343448
343448
346256
330860
———
338907
———
338907
———
———
———
———
———
———
303392
303307
303392
303307
303392
303307
233 000
233 000
233 000
233 000
233 000
233 000
189 000
209 000
187 000
206 000
187 000
206 000
50
50
79
79
50
79
76
94
52
89
52
89
50 000
50 000
50 000
50 000
50 000
50 000
50 000
50 000
50 000
50 000
50 000
50 000
Note: To help you locate g-Max tubes in the rotor listings, these tubes are highlighted with bold, italic type.
§§
Set of 2.
§
To simplify ordering, this g-Max Kit includes 50 tubes, 12 spacers, a spacer removal tool and forceps
Rotor Accessories
Rotor Replacement Parts
306812 Spinkote Lubricant (2 oz)
331202 Hex Driver for 11-mm Cap Nuts on Stainless Steel Tubes
331202 Tool Kit for Aluminum Caps
Includes:
301875 Removal Tool, for capped tubes
858121 Torque Wrench
858122 Socket Adapter
858123 Socket for 19 mm Hex Nut
870432 Socket for 11 mm Hex Nut
335148 Silicone Vacuum Grease
338765 Floating Spacer Removal Tool
339379 Rotor Cleaning Brush
339555 Beckman Solution 555 (1 qt)
339558 Rotor Cleaning Kit
348122 Tube Topper Rack
348123 Tube Topper Rack
348124 Quick-Seal Tube Topper Rack
358312 Quick-Seal Cordless Tube Topper Kit, 60 Hz
358313 Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
358314 Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
358315 Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
361646 OptiSeal Tube Rack Assembly
361668 Tube Removal Tool for Quick-Seal and OptiSeal Tubes
011521
A91766
A92167
A96399
Handle O-Ring (Small)
Rotor Lid
Lid O-Ring (Large)
Rotor Handle
Adapters/Spacers
303307
303392
330860
338907
Beckman Coulter Ultracentrifuge
343448
346256
361669
1-53
1
www.beckmancoultercentrifugation.com
TLN-120
TLN-120
8 x 1.2 mL
NVT Near-Vertical Rotor, Titanium
Axis of Rotation
1
rmin (24.3 mm)
For use in the TL-100*, Optima TL, Optima TLX, Optima MAXXP and Optima MAX-TL ultracentrifuges.
rav (30.3 mm)
Major applications: Plasmid DNA Separations in 11/2 hours.**
Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a
reduction in rotor speed.
rmax (36.3 mm)
8°
Max. RPM
Max. g
k Factor
120 000
585 000
7
Number of Tubes
Volume/Size
Rotor Capacity
8 x 1.2 mL
8 x 35 mm
9.6 mL
No. 357683. TLN-120 Near-Vertical Package. Includes Rotor, 1 box
361082 Quick-Seal Polyallomer Tubes, 10 each 361061 Spacers, 12 each
347371 Rotor Plug Gaskets, 1 each 348302 Tube Rack, 347373 Rotor
Vise/Stand, 927208 Hemostats, 858121 Torque Wrench, 976959 Torx
Wrench Adapter, and 306812 Spinkote Lubricant.
Tubes and Bottles
Tube Style/Material
Quick-Seal
Polyallomer
Vol. (mL)
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
1.2
361082
50
8 x 35
361061
———
585 000
k Factor
7
Max.
Speed
120 000
** To accommodate the TLN-120 Rotor, the TL-100 ultracentrifuge must be updated
withanewdrivespindleandoperatingsoftware(modificationkitnumber360477).
** Using Optima MAX-XP, Optima MAX-TL or Optima TLX.
Rotor Accessories
Rotor Replacement Parts
306812
338765
339555
339558
347402
347404
347960
348299
348304
349661
354526
358312
358313
358314
358315
858121
927208
977305
347371
347373
349339
368549
368550
976959
Spinkote Lubricant (2 oz)
Tube Removal Tool
Beckman Solution 555 (1 qt)
Rotor Cleaning Kit
Rotor Vise Mounting Tape (replacement)
Rotor Cleaning Brush
Centri-Tube Slicer
CentriTube Slicer Replacement Blades (pkg of 10)
Tube Rack, 8 mm
Quick-Seal Tube Sealing Rack
CentriTube Slicer Adapter for 13mm tubes
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
Torque Wrench
Hemostat, 6 in. curved
Rotor Vise Mounting Screws (pkg of 4)
Rotor Plug Gasket
Rotor Vise
Cap and Plunger Assembly
Rotor Plug Replacement (each)
Rotor Replacement Plugs with Gaskets (set of 8)
Torx Wrench Adapter
Adapters/Spacers
361061
1-54
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TLN-100
TLN-100
8 x 3.9 mL
TLN 100
NVT Near-Vertical Rotor, Titanium
Axis of Rotation
For use in the TL-100, Optima TL, Optima TLX, Optima MAX-XP
and Optima MAX-TL ultracentrifuges.
rmin (23.1 mm)
rav (31.6 mm)
rmax (40.2 mm)
Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a
reduction in rotor speed.
Major applications: Plasmid DNA-Separations in 4 hours.
9°
Max. RPM
Max. g
k Factor
100 000
450 000
14
Number of Tubes
Volume/Size
Rotor Capacity
8 x 3.9 mL
13 x 38 mm
31.2 mL
No. 357614. TLN-100 Near-Vertical Rotor Package. Includes Rotor,
1 each 361664 OptiSeal Tube Kit (includes 4 boxes 361627 OptiSeal Tubes,
8 Spacers 362198, 1 Tube Rack 361650, 1 Tube Extraction Tool 361668,
and 1 Spacer Removal Tool 338765), 12 each 342882 Rotor Plug Gaskets,
1 each 348122 and 355872 Tube Racks, 347373 Rotor Vise/Stand, 927208
Hemostats, 858121 Torque Wrench, 976959 Torx Wrench Adapter, 306812
Spinkote Lubricant, and 335148 Vacuum Grease.
Tubes and Bottles
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Vol. (mL)
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
3.3
361627
56
13 x 33
362198
———
450 000
14
100 000
3.9
358980
50
13 x 38
342883
———
450 000
14
100 000
Rotor Accessories
Rotor Replacement Parts
306812
335148
338765
339555
339558
342025
342883
347404
347828
347960
348122
348299
354526
355872
358312
358313
358314
358315
361650
361668
858121
927208
961519
342882
347373
347402
349339
368545
368546
976959
Spinkote Lubricant (2 oz)
Silicone Vacuum Grease (1 oz)
Tube Removal Tool
Beckman Solution 555 (1qt)
Rotor Cleaning Kit
Fraction Recovery System
Quick-Seal Tube Spacer
Rotor Cleaning Brush
Fraction Recovery System Adapter Kit for TL-series tubes
CentriTube Slicer
Tube Topper Rack, 13 mm
CentriTube Slicer Replacement Blades (pkg of 10)
CentriTube Slicer Adapter for 13mm tubes
Tube Rack, 13 mm
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
OptiSeal Tube Rack Assembly
Tube Removal Tool
Torque Wrench
Hemostat, 6-in curved
Hemostat, 6 in. straight
k Factor
Max.
Speed
Rotor Plug Gasket
Rotor Vise
Rotor Vise Mounting Tape (replacement)
Cap and Plunger Assembly
Rotor Replacement Plug (single)
Rotor Replacement Plugs with Gaskets (set of 8)
Torx Wrench Adapter
Adapters/Spacers
342883
362198
Beckman Coulter Ultracentrifuge
1-55
1
www.beckmancoultercentrifugation.com
MLN-80
MLN-80
8 x 8 mL
TLN 100
Near-Vertical Tube Rotor, Titanium
Axis of Rotation
For use in the Optima MAX-XP ultracentrifuge..
Major applications: Rapid contamination-free isopycnic isolation of
plasmid DNA.
rmin (32.5 mm)
rav (43.3 mm)
rmax (54.2 mm)
1
Max. RPM
Max. g
k Factor
80 000
389 000
20
Number of Tubes
Volume/Size
Rotor Capacity
8 x 8 mL
16 x 58 mm
9°
64 mL
No. 367100. MLN-80 Near-Vertical Tube Rotor Package. Includes Rotor,
1 box 344621 Quick-Seal Polyallomer Tubes, 10 each 367101 Spacers,
2 each 392083 Plug and Cell, 349290 Washer and Plug, 1 each 348123
Tube Rack, 347373 Rotor Vise/Stand, 927208 Hemostats, 858121 Torque
Wrench, 976959 Torx Wrench Adapter, 338765 Spacer Removal Tool,
306812 Spinkote Lubricant, and 335148 Vacuum Grease.
No. 367099. MLN-80 Near-Vertical Tube Rotor Assembly.
Tubes and Bottles
Tube Style/Material
Vol. (mL)
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 1)
g-Force
4.2
356562
50
16 x 38
———
389 000
16
80 000
6.3
345830
50
16 x 45
———
389 000
18
80 000
8.0
344621
50
16 x 58
349289
367312
349289
367313
367101
———
389 000
20
80 000
Quick-Seal
Polyallomer
Rotor Accessories
Rotor Replacement Parts
306812
335148
338765
339379
339555
339558
348123
358312
358313
358314
358315
361668
858121
347373
349290
392083
976959
Spinkote Lubricant (2 oz)
Silicone Vacuum Grease (1 oz)
Floating Spacer Removal Tool
Rotor Cleaning Brush
Beckman Solution 555 (1qt)
Rotor Cleaning Kit
Tube Topper Rack, 16 mm
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
Tube Removal Tool
Torque Wrench
k Factor
Max.
Speed
Rotor Vise Assembly
Rotor Plug Gasket
Plug, Cell
Torx Wrench Adapter
Adapters/Spacers
349289
1-56
367101
367312
367313
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TLS-55
TLS-55
4 x 2.2 mL
TLS-55
Swinging-Bucket Rotor, Titanium
For use in the TL-100, Optima TL, Optima TLX, Optima MAX-XP
and Optima MAX-TL ultracentrifuges.
Axis of Rotation
rmax (76.5 mm)
Note: Non-precipitating solutions up to 1.7 g/mL in density can be run in this rotor without a
reduction in rotor speed.
rav (59.4 mm)
Major applications: RNA pelleting in 2-3 hours; subcellular fractionation in sucrose gradients, protein separations in sucrose gradients.
rmin (42.2 mm)
Max. RPM
Max. g
k Factor
55 000
259 000
50
Number of Tubes
Volume/Size
Rotor Capacity
4 x 2.2 mL
11 x 34 mm
8.8 mL
No. 346936. TLS-55 Swinging-Bucket Rotor Assembly with 4 Titanium
Buckets.
No. 346134. TLS-55 Swinging-Bucket Rotor Package. Includes BioCertified Rotor, 1 box 347357 Polyallomer Tubes, 1 each 348305 Tube
Rack, 347358 Bucket Holder Rack, 927208 Hemostats, 868638 O-ring,
306812 Spinkote Lubricant, and 335148 Vacuum Grease.
.Tubes and Bottles
Tube Style/Material
Vol. (mL)
Quick-Seal
Polyallomer
Thinwall
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Thickwall
Cellulose Propionate
Thickwall
Polyethylene
Ultra-Clear
Part No.
Quantity
Size
(mm)
Required Caps/
Spacers (qty. 1)
Required
Adapters (qty. 4)
g-Force
1.5
2.0
175 µL
2.2
1.4
344624
344625
342630
347357
347287
50
50
100
50
100
11 x 25
11 x 32
5 x 20
11 x 34
11 x 34
344674
344674
———
———
———
———
———
358614
———
———
259 000
259 000
248 000
259 000
259 000
37
50
22
50
50
55 000
55 000
55 000
55 000
55 000
230 µL
1.4
230 µL
343775
343778
342303
100
100
100
7 x 20
11 x 34
7 x 20
———
———
———
358615
———
358615
249 000
259 000
249 000
23
50
23
55 000
55 000
55 000
175 µL
343622
100
5 x 20
———
358614
248 000
22
55 000
2.2
347356
50
11 x 34
———
———
259 000
50
55 000
Rotor Accessories
306812
335148
338765
339379
339555
339558
347404
347828
347960
348299
348305
349487
358312
358313
358314
358315
361668
Spinkote Lubricant (2 oz)
Silicone Vacuum Grease (1 oz)
Floating Spacer Removal Tool
Rotor Cleaning Brush
Beckman Solution 555 (1qt)
Rotor Cleaning Kit
Rotor Cleaning Brush
Fraction Recovery System Adapter Kit for TL-series tubes
CentriTube Slicer
CentriTube Slicer Replacement Blades (pkg of 10)
Tube Rack for 11-mm Tubes (included with Rotor Package)
Quick-Seal Tube Rack (Tube Rack Adapters required)√√
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
Quick-Seal Tube Removal Tool
k Factor
Max.
Speed
362100 Tube Rack Adapters, set/8, for 344624 11 x 25 mm
Quick-Seal Tubes√√
362101 Tube Rack Adapters, set/8, for 344625 11 x 25 mm
Quick-Seal Tubes√√
927208 Hemostats, 6-in. curved
√ √ Required for obsolete Bench Quick Sealer.
Rotor Replacement Parts
345770
345773
346364
347358
349338
868638
Bucket Cap Assembly (each)
Rotor Stand
Replacement Bucket Set, set of 4
Bucket Holder Rack
Cap & Plunger Assembly
Bucket O-ring
Adapters/Spacers
344674
358614
358615
Beckman Coulter Ultracentrifuge
1-57
1
www.beckmancoultercentrifugation.com
MLS-50
4 x 5 mL
Swinging-Bucket Rotor, Aluminum
For use in the Optima MAX-XP ultracentrifuge.
Major applications: Rate-zonal separations of subcellular organelles,
pelleting and isopycnic separations of RNA.
1
rmin
rav
rmax
(47.5 mm)
(71.7 mm)
Max. RPM
Max. g
k Factor
50 000
268 000
71
Number of Tubes
Volume/Size
Rotor Capacity
4 x 5 mL
13 x 51 mm
(95.8 mm)
20 mL
Axis of Rotation
No. 367280. MLS-50 Swinging-Bucket Rotor Package. Includes Rotor,
1 box 326819 Polyallomer Tubes, 1 each 331313 Bucket Holder Rack,
927208 Hemostats, 824412 O-ring, 306812 Spinkote Lubricant, and
335148 Vacuum Grease.
No. 367279. MLS-50 Swinging-Bucket Rotor Assembly.
Tubes
Tube Style/Material
OptiSeal
Polyallomer
Quick-Seal
Polyallomer
Quick-Seal
konical
Polyallomer
Thinwall Polyallomer
Thinwall konical
Polyallomer
Thickwall
Polyallomer
Thickwall
Polycarbonate
Ultra-Clear
Vol. (mL)
Part No.
Quantity
Size
(mm)
Floating
Spacers (qty. 1)
Adapters
(qty. 1)
g-Force
3.3
361627
56
13 x 33
———
268 000
42
50 000
2.0
345829
50
13 x 25
361678
(pkg of 2)
355535
———
268 000
29
50 000
3.2
358647
50
13 x 51
355535
358153
(pkg of 6)
262 000
67
50 000
5.0
3.0
326819
358119
50
50
13 x 51
13 x 51
———
———
268 000
262 000
71
67
50 000
50 000
3.5
349623
25
13 x 51
———
———
358153
(pkg of 6)
———
268 000
71
50 000
3.5
349622
25
13 x 51
———
———
268 000
71
50 000
0.8
5.0
344090
344057
50
50
5 x 41
13 x 51
———
———
356860
———
218 000
268 000
67
71
46 000
50 000
Rotor Accessories
Rotor Replacement Parts
306812
331313
335148
338765
339379
339555
339558
347828
347960
348122
348299
354468
358312
358313
358314
358315
361638
361668
367278
367284
367285
824412
Spinkote Lubricant (2 oz)
Bucket Rack
Silicone Vacuum Grease (1 oz)
Floating Spacer Removal Tool
Rotor Cleaning Brush
Beckman Solution 555 (1qt)
Rotor Cleaning Kit
Fraction Recovery System Adapter Kit for TL-series tubes
CentriTube Slicer
Tube Topper Rack for 13-mm Tubes
CentriTube Slicer Replacement Blades (pkg of 10)
Conical Adapter Extractor Tool
Quick-Seal Cordless Tube Topper Kit, 60 Hz
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Europe)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Great Britain)
Quick-Seal Cordless Tube Topper Kit, 50 Hz (Australia)
OptiSeal Tube Rack, 13 mm
Quick-Seal and OptiSeal Tube Removal Tool
k Factor
Max.
Speed
Rotor Stand Assembly
Bucket Set
Bucket Cap
O-ring
Adapters/Spacers
355535
1-58
356860
358153
361678
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
A-110
A-110
6 x 180 µL
A-110
Fixed-Angle Rotor, Aluminum, Red
Axis of Rotation
For use in the Airfuge Air-Driven Micro-Ultracentrifuge.
Major application: Fast pelleting of very small samples.
rmin (9.5 mm)
rav (12.1 mm)
rmax (14.7 mm)
Max. RPM
Max. g
110 000
199 000
k Factor
9
Number of Tubes
Volume/Size
Rotor Capacity
6 x 180 µL
5 x 20 mm
1 080 µL
g-Force
k Factor
Max. Speed
199 000
199 000
199 000
9
9
9
18°
No. 347596. A-110 Rotor. Fixed-Angle Rotor with 18° angle, aluminum, anodized red.
Includes 1 box of 342630 polyallomer tubes and 1 Tube Rack 348301.
Tubes and Bottles
Tube Style/Material
Usable Volume
(µL)
Part No.
Quantity
175
175
180
342630
343622
344718
100
100
100
Size
(mm)
Open-Top Tubes
Polyallomer
Polyethylene
Ultra-Clear
5 x 20
5 x 20
5 x 20
110 000
110 000
110 000
Rotor Supplies
339643
348301
343773
339639
341252
341252
Rotor Caps, polyethylene, package of 20
Tube Rack for 5-mm diameter Tubes
Tube Cap Strip, 6-place, package of 50
Rotor Base Bushing, white
Rotor Stand/Vise
Rotor Stand/Vise
A-100/30
A-100/30
6 x 240 µL
A-100 30
Fixed-Angle Rotor, Aluminum, Blue
Axis of Rotation
For use in Airfuge Air-Driven Micro-Ultracentrifuge.
Major application: Fast pelleting of very small samples.
rmin (8.8 mm)
rav (13.2 mm)
rmax (17.6 mm)
Max. RPM
Max. g
k Factor
92 000
167 000
19
Number of Tubes
Volume/Size
Rotor Capacity
6 x 240 µL
5 x 20 mm
1 440 µL
k Factor
Max. Speed
30°
No. 347594. A-100/30 Rotor. Fixed-Angle Rotor with 30° angle, anodized blue.
Includes 1 box of 342630 Polyallomer Tubes and 1 Tube Rack 348301.
Tubes and Bottles
Tube Style/Material
Usable Volume
(µL)
Part No.
Quantity
220
150
240
342630
343622
344718
100
100
100
Size
(mm)
g-Force
Open-Top Tubes
Polyallomer
Polyethylene
Ultra-Clear
5 x 20
5 x 20
5 x 20
167 000
167 000
167 000
19
19
19
92 000
92 000
92 000
Rotor Supplies
339643
341252
343773
348301
Rotor Caps, polyethylene, package of 20
Rotor Stand/Vise
Tube Cap Strip, 6-place, package of 50
Tube Rack for 5-mm diameter Tubes
Beckman Coulter Ultracentrifuge
1-59
1
www.beckmancoultercentrifugation.com
A-100/18
Fixed-Angle Rotor, Aluminum, Silver
Axis of Rotation
For use in the Airfuge Air-Driven Micro-Ultracentrifuge.
Major application: Fast pelleting of very small samples.
rmin (9.5 mm)
rav (12.1 mm)
rmax (14.7 mm)
1
A-100/18
6 x 175 µL
A-100 18
Max. RPM
Max. g
k Factor
95 000
148 000
12
Number of Tubes
Volume/Size
Rotor Capacity
6 x 175 µL
5 x 20 mm
1 050 µL
k Factor
Max. Speed
18°
No. 347593. A-100/18 Rotor. Fixed-Angle Rotor with 18° angle, aluminum, anodized
silver. Includes 1 box of 342630 Polyallomer Tubes and 1 Tube Rack 348301.
Tubes and Bottles
Tube Style/Material
Usable Volume
(µL)
Part No.
Quantity
175
100
175
342630
343622
344718
100
100
100
Size
(mm)
g-Force
Open-Top Tubes
Polyallomer
Polyethylene
Ultra-Clear
5 x 20
5 x 20
5 x 20
148 000
148 000
148 000
12
12
12
95 000
95 000
95 000
Rotor Supplies
339643
348301
343773
339639
341252
Rotor Caps, polyethylene, package of 20
Tube Rack for 5-mm diameter tubes
Tube Cap Strip, 6-place, package of 50
Rotor Base Bushing, white
Rotor Stand/Vise
A-95
A-95
4 x 450 µL
Fixed-Angle Rotor, Aluminum, Black
Axis of Rotation
For use in Airfuge Air-Driven Micro-Ultracentrifuge.
Major application: Fast pelleting of very small samples.
rmin (8.9 mm)
rav (13.3 mm)
rmax (17.6 mm)
Max. RPM
Max. g
k Factor
95 000
178 000
19
30°
Number of Tubes
Volume/Size
Rotor Capacity
4 x 450 µL
8 x 20 mm
1 800 µL
k Factor
Max. Speed
No. 347595. A-95 Rotor. Fixed-Angle Rotor with 30° angle, aluminum, anodized
black. Includes 1 box of 345843 Ultra-Clear Tubes and 1 Tube Rack 348302.
Tubes and Bottles
Tube Style/Material
Usable Volume
(µL)
Part No.
Quantity
450
345843
100
Size
(mm)
g-Force
Open-Top Tubes
Ultra-Clear
8 x 20
178 000
19
95 000
Rotor Supplies
339643
339639
341252
343773
348302
1-60
Rotor Caps, polyethylene, package of 20
Rotor Base Bushing, white
Rotor Stand/Vise
Tube Cap Strip, 6-place, package of 50
Tube Rack for 8-mm diameter Tubes
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
ACR-90
ACR-90
3.5 mL/2.4 mL
ACR-90 Chylomicron Rotor, Silver
For use in the Airfuge Air-Driven Micro-Ultracentrifuge.
Major application: Rapid clarification of lipemic blood samples.
Max. RPM
Max. g
k Factor
Rotor Capacity
Volume/Size
Yield
(mL)
90,000
90,000
122 000
107 000
45
39
3.5 mL
2.4 mL
2.6
1.4
1
No. 341260. ACR-90 Chylomicron Rotor, anodized aluminum. Consists
of a Supporting Base, a Disposable Polyethylene Liner, and Stainless Steel
Lid. Includes one 341252 Rotor Stand/Vise, one package 341251 Liners,
Adapter for 342635 Liner.
Rotor Supplies
306812
339639
341251
341252
342634
Spinkote Lubricant
Rotor Bushing, white
Liner, polyethylene, 3.5 mL capacity, package of 100
Rotor Stand/Vise
Liner, polyethylene, 2.4 mL capacity, package of 100
(Adapter required)
342635 Adapter for 342634 Liners, reusable
343779 Disposable Loading and Unloading Pipette, package of 500
Batch Rotor
Batch Rotor
7 mL
Batch Rotor, Aluminum, Silver
For use in the Airfuge Air-Driven Micro-Ultracentrifuge.
Major application: Rapid processing of fruit paste.
Max. RPM
Max. g
Minimum
Fill Volume
Maximum
Fill Volume
k Factor
90 000
132 000
2 mL
7 mL
8 at 2 mL
55 at 7 mL
No. 347833. Batch Rotor, aluminum, for 2- to 7-mL volumes. Sample
is placed in the rotor body itself or in one of two types of polyethylene
liners. A plastic window disk is placed between the rotor and the lid to
contain samples during centrifugation. Includes 1 each 341252 Rotor
Vise/Stand, 853156 O-ring, and 343197 Bushing.
Rotor Supplies
341252 Rotor Vise/Stand
343130 Shell Liner, polyethylene, large opening, 7-mL capacity,
package of 100
343132 Dome Liner, polyethylene, small opening, 7-mL capacity,
package of 100
343197 Bushing, red
347049 Rotor Window Disk
853156 Rotor O-ring
Beckman Coulter Ultracentrifuge
1-61
www.beckmancoultercentrifugation.com
EM-90
EM-90
6 x 100 µL
EM-90 Particle Counting Rotor, Silver
For use in the Airfuge Air-Driven Micro-Ultracentrifuge.
Major applications: Sedimenting particles onto filter membranes, glass
cover slides, and microscope grids.
1
Max. RPM
90 000
Max. g
Maximum
Volume
Rotor Capacity
118 000
6 x 100 µL
600 µL
No. 347844. EM-90 Electron Microscopy Particle Counting Rotor.
Includes 5-mm Core with 600 µL capacity (100 µL per cell); 3-mm Core
with 360 µL capacity (60 µL per cell); one 341252 Rotor Stand/Vise, one
347840 (pkg of 5) Gaskets, Sector Core Removal Bolt, Template, one
853156 O-ring, one 343197 Bushing.
Rotor Supplies
343197
347836
347838
347840
Bushing, red
Sector Core, 5 mm
Sector Core, 3 mm
Rotor Gaskets, package of 5
Lid
Core Cover
Flat Gasket
Sector Core
(3 mm or 5 mm)
Rotor Base
Stand
1-62
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Tubes and Bottles
Tubes and Bottles For Every Application
N
2
o single tube design or material will meet all
application requirements. A number of factors
should be considered at the time a supply of tubes
is ordered: the particular technique to be used, the nature of
the sample and any solvent or gradient media, the desirabil­
ity of reusing the tubes, and certain convenience factors.
The properties listed below provide a guide for anyone
involved in the tube selection process.
• Strength and Flexibility, to resist permanent deformation even
whenruninfixedanglerotorswithouttubecaps.
• Chemical Resistancetoawiderangeofbases,acids,andsolvents.
• Transparency,topermitaclearviewoffractionsandbandsafter
centrifugation.
• Thinenoughtobeslicedorpuncturedaftercentrifugationfor
fractioncollection.
• Impermeable to Water,topreventaqueoussolutionsfrom
permeatingthetubewallandreachingtherotorcavity.
• Surface Propertiesthatpreventtheadherenceofnucleicacids
andproteins.
• Temperature Tolerancethroughoutawiderangeofoperating temperatures,withoutdeformingathightemperaturesorcracking
whenusedcloseto0°C.
• Autoclavable,forconvenientsterilizationandreuse.
• Contaminant-free,toavoidleachingextraneousmaterialsinto
thesample,especiallymaterialsvisibleinthesensitive240-280nmrange.
• Odor-free,forpleasanthandling.
The full line of Beckman Coulter tubes includes a number
of tube materials, each with its own distinct combination of
properties, to meet a variety of application requirements.
Available are transparent, translucent, and opaque tubes,
tubes that can be sliced or punctured, tubes that can be
sterilized and reused, and tubes that are resistant to a variety
of chemical compounds.
Beckman Coulter Ultracentrifuge
2-1
www.beckmancoultercentrifugation.com
TubesandBottles
Tube Selection Considerations
2
Compatibility of Tube Material with Solvents and Sample
Thechemicalcompatibilityofthetubematerialswiththe
gradient-formingmediumorothersolventisaprimeconsideration.Neutralsucroseandsaltsolutionscausenoproblem.
Butalkalinesolutions,suchasthosefrequentlyusedfor
theseparationofsingle-strandedformsofDNA,cannot
beusedinUltra-Cleartubesorpolycarbonatetubesand
bottles.SometimesDMSOisusedinpreparationofsucrose
gradientsforsedimentationofdenaturedRNA.Polycarbonate
andUltra-CleartubesareincompatiblewithDMSO,so
polyallomertubesshouldbeused.
Thelastcolumnofthe“QuickReferenceCharttoTube
MaterialsandTheirProperties”onpage2-4givessomeguidelinestothechemicalresistancesofthevarioustubematerials.
Itmustbeemphasized,however,thatotherconditionsofcentrifugation(g-force,durationofrun,etc.)haveconsiderable
effectonhowwellatubematerialwillwithstandaparticular
solvent.BeckmanCoulterpublicationIN-175,“Chemical
ResistancesforBeckmanCoulterCentrifugationProducts”
(foundontheBeckmanCoulterwebsiteat
www.beckmanbuilt.comprovidesmoredetailed
informationaboutthechemicalresistancesofthevarious
tubematerials.Thewisestcourseistotestanyquestionable
combinationunderoperatingconditionsbeforemakingthe
actualrun.
Thetypeofsample,insomecases,willaffectselectionofa
specifictubematerial.DNA,initsdenaturedorsinglestrandedform,willadheretothesurfaceofsometube
materials.Polyallomerwouldbethebestchoice.(Mostofthis
workisdoneinhighlyalkalinemediawhichareincompatible
withpolycarbonate.)
LipoproteinseparationsaremostoftendoneinUltra-Clear
tubesbecausetheyareclearandsliceable;theseproperties
simplifyfractionlocationandrecoverybytubeslicing.
Whensmalllipoproteinsamplesaretoberecoveredbya
fractionatingdeviceandcleartubesaredesirable,thereare
alternatives:cellulosepropionate,polycarbonate,andUltraCleartubes.
Hazardousmaterials,eitherpathogenicorradioactive,
shouldbecentrifugedwithextremecare.Allpossible
precautionsmustbetakentoavoidleakageofthesampleinto
therotorcavityduringcentrifugation.
Todeterminetheoptimumtubematerialforyourspecific
sampleandgradientmedium,refertothequickreference
chartonpage2-4.
Astherearecurrentlynowettableplasticcentrifugetubes
available,gradientsshouldbeloadedintoplastictubesfrom
thebottomuptoavoidmixing.
High Temperature Centrifugation
Althoughmoderncentrifugesandrotorscanoperateat
temperaturesashighas45°C,onecannotassumethatevery
tubecanbesafelyrunover25°C.Stainlesssteelandglassare
theonlymaterialswhichwillnotexperiencesomedeformation
whensubjectedtohightemperaturesandlongcentrifugation
times.Plastictubesundergosomedegreeofsofteningat
temperatureshigherthan25°C.Whetherornotthiswillcause
permanentdeformationisnotaquestionoftemperaturealone.
Thecentrifugalforcefieldused,thedurationofthecentrifugation,thetypeofrotor,andeventhetubeangleallhavean
effect.
It’sobviouslyimpossibletogiveexacttemperaturelimits
forplastictubeswhensomanyothervariablesareinvolved.
Thesafestpolicyistopretestthetubesundertheactual
experimentalconditions,butwithwater,ratherthanavaluable
sample.
Tube Sizes
Tubesizesasindicatedinthefollowingchartsarenominal
sizes,andmayvarysomewhatfromactualfillingcapacities.
Ifathick-walledtubeisrununcapped,themaximumfilling
volumewilldependonthetubeangleoftherotortobeused.
Seeappropriaterotorinstructionmanualsformaximumfilling
levelsoftubes.
Tube Cleaning, Sterilization, and Reuse
Iftubesaretobereused,specialcaremustbetakenduring
cleaningandsterilization.Alltubescanbewashedbyhand
withamilddetergentsuchasSolution555diluted5-to-1or
10-to-1withwater.Thisisparticularlyimportantfor
polycarbonatetubesandbottleswhichshouldnotbeexposed
toadetergentwithapHhigherthan8.Tubesandbottles
shouldnotbewashedincommercialdishwashersasthe
detergentsandhightemperaturesaretooharsh.Solvents
suchasalcoholoracetonereactunfavorablywithmanytube
materials.Ifanorganicsolventmustbeusedinthecleaning
procedure,consultbulletinIN-175foratableoftubematerial/
solventcompatibilities(orreviewthesamedocumentonthe
BeckmanCoulterwebsiteatwww.beckmanbuilt.com.
Themethodchosenforsterilizationhasdirectbearingon
thenumberofreusesonecanexpectfromatube.Tubesand
Gradient Formation and Fractionation
bottlesofpolyallomer,polyethylene,andglasscanallbe
Whenchoosingatubeforadensitygradientrun,somethought
autoclaved,althoughingeneral,coldsterilizationmethodsare
shouldbegiventogradientformationandfractionation.Ifthe
notasharshasautoclaving.Coldsterilizationisrecommended
bandsorzonesformedduringcentrifugationareindistinct,
forbothpolycarbonateandUltra-Clear.Donotdrytubes,
theymaynotbevisiblethroughatranslucentmaterialsuch
bottles,oraccessoriesinanoven.Labwareshouldbeairdried.
aspolyallomer.Ifoptimumbandvisualizationisimportant,
OptiSeal,Quick-Seal,UltraClear,andthinwallpolyallomer
Ultra-Cleartubesortubesofpolycarbonateorcellulose
tubesareintendedforone-timeuseandshouldbediscarded
propionateshouldbeused.Whenevercollectionofbandsor
afteruse.
zonesmustbedonebypuncturingthetubeorslicing,athin,
Ifmaximumreuseisamajorconsideration,either
flexibletubewallisrequired.Ultra-Clearorpolyallomertubes
polyallomer(preferablythick-walled)orpolycarbonatetubes
shouldbeused,dependingontheneedfortransparency.
2-2
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TubesandBottles
andbottlesshouldbeselected,andcoldsterilizationmethods
used.Ifthesetubesareruncompletelyfilledinswinging
bucketrotors,mostofthemcanbereusedanumberoftimes.
Chancesofpermanentdeformationwillbegreaterwhenever
thetubesareruninfixedanglerotors,withoutcaps,and/
orpartiallyfilled.Alloftheseconditionstendtostressthe
centripetaledgeofthetubeunduly.Alltubesthathave
beenusedorautoclavedpreviouslymustbeindividually
examinedforsignsofdeformationorcrackingbeforeusing
themagain.
Tube Closures
Whenotherconsiderationshavebeenresolved,
conveniencemaybeadecidingfactor.Withoutadoubt,
themostconvenienttubeclosureisnoneatall;noneare
requiredfortubesruninswingingbucketrotorsandinthe
AirfugeAir-DriveMicro-Ultracentrifuge.
Fortubesruninfixedanglerotors,alternativestothe
standardtubecapassembliesareavailable.Bottleshave
three-piececapassemblieswhichareeasiertousethanthe
morecomplextubecapassemblies.Polycarbonatebottles
areavailableforgeneral-purposefixedanglerotors,andare
usedfrequentlyfordifferentialcentrifugationwhereband
recoveryisnotaproblem.Thickwalltubescanberuninall
fixedanglerotorswithoutcaps,providedtheyarepartially
filled.(Refertorotormanualsformoreinformationon
fillvolumes.)
Whenclosedtubesarerequired,BeckmanCoulteroffers
someinnovativeandconvenient
options.
OptiSeal Tubes
Forvirtuallyeffortlesssealing,
OptiSealtubesofferthe
bestoption.Yousimply
insertthetubeplugand
press,andanO-ring
sealssecurelyagainst
thetube’sinnersurface.
Duringcentrifugation,the
combinationofg-force
andhydrostaticpressure
ensuresaneffectiveseal.
EngineeredforreliabilCentrifugal
ity,therearenotools
force
ormechanicalpartsto
maintain.Fingerpressure
isallthatisneeded.
Beckman Coulter Ultracentrifuge
Hydrostatic
pressure
Quick-Seal Tubes
Thesetubeseliminatemostofthestepsinvolvedin
cappingtubesandcuthandlingtimeinhalf.Thetopofthe
Quick-Sealtubeiseitherdome-shapedorbell-shapedwitha
3-mm-longinletthroughwhichthetubeisfilled.Thefilled
tubeisheat-sealedusing
thehand-heldsealer.
Thehighlyreliableseal
achievedmakesthese
Dome-top Tubes
tubesidealforsample
storageandforworking
withradioactiveor
pathogenicsamples.
Bell-top Tubes
There are two
Quick-Sealtubedesigns—dome-topandbell-top.Thebelltopsimplifiesremovalofmaterialsthatfloatuponcentrifugation.Thedome-toptubesholdmorevolumethantheir
bell-topequivalents.
Tube plug
is forced into
stem by spacer
Tube stem
elongates
due to
hydrostatic
pressure
2-3
2
www.beckmancoultercentrifugation.com
TubesandBottles
A Quick-Reference Chart to Tube Materials and Their Properties
2
Property
Thinwall
Polyallomer
Thickwall
Polyallomer
Ultra-Clear
Optical
Polycarbonate Polypropylene
Polyethylene
Cellulose
Propionate
transparent
translucent
transparent
transparent
translucent
transparent/translucent
transparent
Autoclaveable
yes
yes
no
no
yes
no
no
Puncturable
yes
no
yes
no
no
yes
no
Sliceable
yes
no*
yes
no*
no
no
no*
Reusable
no
yes
no
yes
yes
yes
no
Acids (dilute
or weak)
S
S
S
S
S
S
S
Acids (strong)
U
S
U
U
S
S
U
U
Alcohols
(aliphatic)
U
S
U
U
S
S
Aldehydes
M
M
S
M
M
S
U
Bases
S
S
U
U
S
S
U
Esters
U
M
U
U
M
S
M
Hydrocarbons
(aliphatic)
U
M
U
U
S
U
S
Hydrocarbons
(aromatic and
halogenated)
U
U
U
U
M
M
S
Ketones
U
M
U
U
M
M
U
Oxidizing
Agents (strong)
U
U
U
M
M
M
M
Salts
S
S
M
M
S
S
S
S = satisfactory resistance
M = marginal resistance
U = unsatisfactory resistance
Note: This information has been consolidated from a number of sources and is provided only as a guide to the selection of tube materials. Soak tests at 1 g (at 20°C)
established the data for most of the materials; reactions may vary under the stress of centrifugation, or with extended contact or temperature variations. To prevent
failure and loss of valuable sample, ALWAYS TEST SOLUTIONS UNDER OPERATING CONDITIONS BEFORE USE.
* Polyallomer, polycarbonate, and cellulose propionate tubes with diameters of 5 to 13 mm may be sliced using the CentriTube Slicer (part number 347960) and appropriate
adapter plate.
Warning:Donotuseflammablesubstancesinornearanoperatingcentrifuge.
General Filling and Sealing Requirements for Tubes and Bottles
Tube or Bottle
Swinging-Bucket Rotors
Fixed-Angle Rotors
Thinwall tubes
Within 2 to 3 mm of top
Full with cap
Thickwall tubes
At least 1/2 full
1/2 full to max. capless level or full with cap
Quick-Seal tubes
Full and heat-sealed
Full and heat-sealed
Bottles
Min. to max. (see rotor manual) with
screw-on cap or cap assembly
1/2 full to max. (see rotor manual)
with screw-on cap assembly
Open-top tubes
Within 2 to 3 mm of top
Full with cap
Quick-Seal tubes
Not used
Full and heat-sealed
Thickwall tubes
At least 1/2 full
1/2 full capless level or full with cap or cap assembly
Bottles
At least 1/2 full
Min. to max. (see rotor manual)
with screw-on cap or cap assembly
Stainless Steel
Tubes
Any level
Any level with cap or cap assembly
Polypropylene
Tubes and bottles
At least 1/2 full
1/2 to max. capless level or
full with cap or cap assembly
Polyethylene
Tubes
At least 1/2 full
1/2 to max. capless level or full with cap or cap assembly
Teflon
Tubes and bottles
At least 1/2 full
1/2 full to max. capless level or full with cap
Radel
Container
At least 1/2 full
N/A
Polyallomer
Ultra-Clear
Polycarbonate
2-4
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TubesandBottles
Nominal
Filling
Capacity (mL)
Nominal
Size
(mm)
Part
No.
Rotors
See specific rotor pages for required spacers/adapters/accessories
OptiSeal Polyallomer Tubes
3.3
13 x 33
361627
SW-55 Ti, SW-50.1, TLN-100, MLS-50
4.7
13 x 48
361621
Types 50.4 Ti, 50.3 Ti, TLA-110, TLA-100.4
4.9
13 x 51
362185
VTi 90, VTi 65.2, NVT 90, NVT 65.2
8.9
16 x 60
361623
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti, 50, MLA-55
11.2
16 x 70
362181
VTi 65.1, NVT 65
32.4
25 x 77
361625
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, 42.1, 30, SW 32 Ti, SW 28, MLA-50
36.2
25 x 89
362183
VTi 50
8 x 51
345831
Type 25
1.2
8 x 35
361082
TLN-120
1.5
11 x 25
344624
SW 60 Ti, TLA-120.2, TLA-100.2, MLA-130, MLA-150, TLS-55
2.0
11 x 32
344625
SW 60 Ti, TLA-120.2, TLA-100.2, MLA-130, MLA-150, TLS-55
2.0
13 x 25
345829
Types 100 Ti, 50.4 Ti, 50.3, NVT 100, NVT 90, NVT 65.2, VTi 90, VTi 65.2, SW 55 Ti,
SW 50, TLA-110, TLA-100.4, TLA-100.3, MLS-50
3.5
13 x 32
349621
Types 100 Ti, TLA-110, TLA-100.3
3.5
14 x 25
355870
SW 41 Ti, SW 40 Ti
3.9
13 x 38
358980
TLN-100
4.2
16 x 32
356562
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti, 40, SW 30.1, SW 32.1 Ti, SW 28.1, MLA-80,
MLN-80, MLA-55
5.1
13 x 51
342412
VTi 90, VTi 65.2, NVT 100, NVT 90, NVT 65.2
5.1
13 x 51
362248
Type 100 Ti, TLA-110
5.9
14 x 47
355537
SW 41 Ti, SW 40 Ti
6.0
13 x 64
344619
Types 100 Ti, 50.4 Ti, 50.3 Ti
6.3
16 x 45
345830
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti, 50, 40, NVT 65, VTi 65.1,SW 30.1,
SW 32.1 Ti, SW 28.1, MLA-80, MLN-80, MLA-55
2
Quick-Seal Polyallomer Tubes
1.0
8.0
16 x 58
344621
Type 50, NVT 65, VTi 65.1, SW 32.1 Ti, SW-28.1, MLA-80, MLN-80
10.0
16 x 67
344622
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti ,40, NVT 65, VTi 65.1, SW 32.1 Ti, SW 28.1, MLA-55
13.5
16 x 76
342413
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti ,40, NVT 65, VTi 65.1, MLA-55
15.0
25 x 38
343664
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, VTi 50, SW 30, SW 32 Ti, SW 28, MLA-50
17.0
16 x 102
356291
SW 32.1 Ti, SW 28.1
27.0
25 x 64
343665
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, 42.1, VTi 50, SW 32 Ti, SW 28, MLA-50
33.0
25 x 83
344623
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, SW 32 Ti, SW 28
39.0
25 x 89
342414
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, VTi 50
100.0
38 x 102
345776
Type 45 Ti
Quick-Seal Polyallomer konical Tubes
1.3
11 x 35
358655
SW 60 Ti
3.0
11 x 60
358648
SW 60 Ti
3.2
13 x 51
358647
SW 55 Ti, SW 50.1 Ti, MLS-50
4.0
14 x 48
358650
SW 41 Ti, SW 40 Ti
8.0
14 x 89
358649
SW 41 Ti, SW 40 Ti
8.5
25 x 38
358652
SW 30, SW 32 Ti, SW 28
12.5
16 x 102
358653
SW 32.1 Ti, SW 28.1
23.0
25 x 76
358654
SW 32 Ti, SW 28
28.0
25 x 83
358651
SW 32 Ti, SW 32 Ti, SW 28
Quick-Seal Ultra-Clear Tubes
5.1
13 x 51
344075
NVT 90, NVT 65.2, VTi 90, VTi 65.2
6.0
13 x 64
344320
Types 50.4 Ti, 50.3 Ti
13.5
16 x 76
344322
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti, 40, NVT 65, VTi 65.1, MLA-55
Beckman Coulter Ultracentrifuge
2-5
www.beckmancoultercentrifugation.com
TubesandBottles
Nominal
Filling
Capacity (mL)
Nominal
Size
(mm)
Part
No.
Rotors
See specific rotor pages for required spacers/adapters/accessories
25 x 38
25 x 64
25 x 89
38 x 102
344324
344323
344326
345778
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, VTi 50, MLA-50
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, VTi 50, MLA-50
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, VTi 50
Type 45 Ti
175 µL
450 µL
0.8
2.0
2.2
3.0
4.0
4.0
5.0
6.5
5 x 20
8 x 20
5 x 41
8 x 49
11 x 34
13 x 32
11 x 60
13 x 41
13 x 51
13 x 64
344718
345843
344090
344091
347356
344092
344062
344093
344057
344088
10.0
10.5
13.2
13.5
16 x 64
13 x 89
14 x 89
16 x 76
344089
344087
344059
344085
14.0
17.0
38.5
94.0
14 x 95
16 x 102
25 x 89
38 x 102
344060
344061
344058
345777
A-110, A-100/30, A-100/18
A-95
SW 55 Ti, SW 50.1, MLS-50
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti, 50.4 Ti, 50.3 Ti, 50, 40, MLA-55
TLS-55
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti, 50, 40, MLA-55
SW 60 Ti
Types 90 Ti, 80 Ti, 70.1 Ti, 65, 50 Ti, 50, 40, MLA-55
SW 55 Ti, SW 50.1, MLS-50
Types 90 Ti, 80 Ti, 70.1 Ti, 70 Ti, 65, 60 Ti, 55.2 Ti, 50.4 Ti, 50.3 Ti, 50.2 Ti, 50 Ti,
45 Ti, 40.3, 40.2, 40, 21, MLA-55
Type 50
Types 45 Ti, 21
SW 41 Ti
Types 90 Ti, 80 Ti, 70.1 Ti, 70 Ti, 65, 60 Ti, 55.2 Ti, 50.2 Ti, 50 Ti, 45 Ti, 40, 21,
MLA-55
SW 40 Ti
SW 32.1 Ti, SW 28.1
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, SW 32 Ti, SW 28
Types 45 Ti, 21
Quick-Seal Ultra-Clear Tubes (cont’d)
15.0
27.0
39.0
100.0
2
Ultra-Clear Tubes
Thinwall Polyallomer Tubes
175 µL
2.2
4.0
5.0
6.5
11 x 34
11 x 60
13 x 51
13 x 64
5 x 20
342630
347357
328874
326819
326820
10.5
13.2
13.5
13 x 89
14 x 89
16 x 76
326822
331372
326814
14.0
17.0
35.5
38.5
94.0
14 x 95
16 x 102
25 x 83
25 x 89
38 x 102
331374
337986
344367
326823
345775
TLS-55, A-110, A-100/30, A-100/18
TLS-55
SW 60 Ti
SW 55 Ti, SW 50.1, MLS-50
Types 90 Ti, 80 Ti, 70.1 Ti, 70 Ti, 65, 60 Ti, 55.2 Ti, 50.4 Ti, 50.3 Ti, 50.2 Ti, 50 Ti,
45 Ti, 40, 40.3, 40.2, 21, MLA-55, MLA-50
Types 45 Ti, 21
SW 41 Ti
Types 90 Ti, 80 Ti, 70.1 Ti, 70 Ti, 65, 60 Ti, 55.2 Ti, 50.2 Ti, 50 Ti, 45 Ti, 40, 30, 21,
MLA-55, MLA-50
SW 40 Ti
SW 32.1 Ti, SW 28.1
Type 70 Ti
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, SW 32 Ti, SW 28
Types 45 Ti, 21
358117
358118
358119
358120
358121
358123
358125
358126
SW 60 Ti
SW 60 Ti
SW 55 Ti, SW 50.1 Ti, MLS-50
SW 41 Ti, SW 40 Ti
SW 40 Ti
SW 32.1 Ti, SW 28.1
SW 32 Ti, SW 28
SW 32 Ti, SW 28
Thinwall Polyallomer, konical Tubes
1.5
3.0
3.0
10.0
11.0
14.5
25.0
30.0
2-6
11 x 35
11 x 60
13 x 51
14 x 89
14 x 95
16 x 102
25 x 76
25 x 89
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TubesandBottles
Nominal
Filling
Capacity (mL)
Nominal
Size
(mm)
Part
No.
Rotors
See specific rotor pages for required spacers/adapters/accessories
Thickwall Polyallomer Tubes
230 µL
7 x 20
343621
Type 42.2 Ti, TLA-100
.5
8 x 34
343777
TLA-120.1, TLA-100.1
1.0
11 x 34
347287
TLA-120.2, TLA-100.2, MLA-130, MLA-150, TLS-55
3.0
11 x 60
355636
SW 60 Ti
3.2
13 x 56
362333
TLA-110, TLA-100.4
3.5
13 x 51
349623
SW 55 Ti, SW 50.1, TLA-100.3, MLS-50
4.0
13 x 64
355644
Types 90 Ti, 80 Ti, 70.1 Ti, 70 Ti, 65, 60 Ti, 55.2 Ti, 50.4 Ti, 50.3 Ti, 50.2 Ti, 50 Ti,
45 Ti, 40, 21, MLA-55, MLA-50
6.5
16 x 64
355646
Type 50, MLA-80
10.0
16 x 76
355640
Types 90 Ti, 80 Ti, 75 Ti, 70.1 Ti, 70 Ti, 65, 60 Ti, 55.2 Ti, 50.2 Ti, 50 Ti,45 Ti,
42.1, 40, 35, 30, 21, SW 25.1, MLA-55, MLA-50
10.5
13 x 89
355639
Types 45 Ti, 21
32.0
25 x 89
355642
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, SW 32 Ti, SW 28
94.0
38 x 102
355643
Types 45 Ti, 21
Thickwall Polycarbonate Tubes
230 µL
7 x 20
343775
Type 42.2 Ti, TLA-100, TLS-55
.5
8 x 34
343776
TLA-120.1, TLA-100.1
1.0
8 x 51
355657
Types 50.4 Ti, 25
1.0
11 x 34
343778
TLA-120.2, TLA-100.2, MLA-130, MLA-150, TLS-55
3.0
11 x 60
355635
SW 60 Ti
3.2
13 x 56
362305
TLA-110, TLA-100.4
3.5
13 x 51
349622
SW 55 Ti, SW 50.1, TLA-100.3, MLS-50
4.0
13 x 64
355645
Types 90 Ti, 80 Ti, 70.1 Ti, 70 Ti, 65, 60 Ti, 55.2 Ti, 50.4 Ti, 50.3 Ti, 50.2 Ti, 50 Ti,
45 Ti, 40, 21, MLA-55, MLA-50
6.5
16 x 64
355647
Type 50, MLA-80
10.0
16 x 76
355630
Types 90 Ti, 80 Ti, 75 Ti, 70.1 Ti, 70 Ti, 65, 60 Ti, 55.2 Ti, 50.2 Ti, 50 Ti, 45 Ti,
40, 21, MLA-55, MLA-50
10.5
13 x 89
355629
Types 45 Ti, 21
32.0
25 x 89
355631
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, SW 32 Ti, SW 28
94.0
38 x 102
355628
Types 45 Ti, 21
7 x 20
342303
Type 42.2 Ti
38.5
25 x 89
301112
Types 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, 42.1,
94.0
38 x 102
303380
Types 45 Ti, 21
5 x 20
343622
TLS-55, A-110, A-100/30, A-100/18
Cellulose Propionate Tubes
230 µL
Stainless Steel Tubes
Polyethylene Tubes
175 µL
Polyallomer Tubes with Snap-on Caps
1.5
11 x 40
Beckman Coulter Ultracentrifuge
357448 (Natural) TLA-110, TLA-100.4, TLA-100.3, TLA-55, TLA-45
2-7
2
www.beckmancoultercentrifugation.com
BottleAssemblieswithThree-PieceCaps
Nominal
Filling
Capacity (mL)
Nominal
Size
(mm)
Bottle
& Cap
Assembly
Bottle
Only
Cap
Assembly
Cap
Only
Plug
Only
O-ring
Only
Rotors
Bottle and Cap Assembly
2
PC — 8.5
16 x 64
355615
355656
355604
335257
335256
870409
Type 50
PC — 10.4
16 x 76
355603
355651
355604
335257
335256
870409
Types 90 Ti, 80 Ti, 75
Ti, 70.1 Ti, 65, 50 Ti, 40,
MLA-55
PC — 26.3
25 x 89
3556161
355654
355617
335259
335258
870385
Type 30
PC — 26.3
25 x 89
3556181
355654
355619
338824
335258
870385
Types 70 Ti, 60 Ti,
55.2 Ti, 50.2 Ti, 42.1
PC — 70.0
38 x 102
3556202
355655
355621
334547
334545
870384
Types 35, 21
PC — 70.0
38 x 102
3556222
355655
355623
339165
334545
870384
Type 45 Ti
PA —250.0
60 x 120
334205
355627
362247
362246
332836
812715
Type 19
PP —250.0
62 x 122
356011
358326
358977
357490
N/A
927860
Type 16
PC —250.0
62 x 122
356013
358275
358977
357490
N/A
927860
Type 16
1
2
Note: Regarding packaging, bottle and cap assemblies are packaged in units of 6, with the following exceptions: Bottle and Cap assembly 334205 is packaged as one; Bottles
355656 and 355651 are packaged in units of 25; Cap assembly 334543 is packaged as one. Caps, Plugs, and O­rings are packaged separately.
The only difference between these two assemblies is the cap which is Noryl in the 355616 assembly and aluminum in the 355618 assembly. The Noryl cap can be used in the
Type 30 Rotor.
The only difference between these two assemblies is the cap which is Noryl in the 355620 assembly and aluminum in the 355622 assembly. The Noryl cap can be used in the
Type 35 and 21 Rotors; the aluminum cap is required for the higher speed forces of the Type 45 Ti.
2-8
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
TubesandBottles
Tube Cap Assemblies and Parts
Cap
Material
Tube
Cap
Hex
Nut
Crown
Set
Screw
Insert
O-ring or
Gasket
Stem
Set Screw
5⁄16
Al
Al
1⁄2
303624
303658
303379
303379
303809
303810
———
———
———
———
303730
303730
303377
303377
303113
346256
305022
301870
301870
301870
307004
307004
307004
———
803543
301870
301870
301870
335320
307006
330774
338911
335319
338864
803543
338864
338864
———
302312
344672
344672
344672
307005
346246
302331
301869
858046
878572
858046
302266
330788
338910
341969
in. (16 mm)
Al
Al
Al
Ti
303319
330860
338907
341968
302312
302312
302312
302312
1 in. (25 mm)
Al
Al
Al
Al/Ti
Al
Al
302359
302133
331151
337927
338901
338906
2
Hex Nut
Washer
in. (13 mm)
Al
Al
Al/SS
5⁄8
Insert
in. (8mm)
Crown
O-ring
or Gasket
Stem
Tube
301870
301870
330791
330791
330791
330791
302169
302169
331153*
338863*
338912*
338915*
338864
338864
338864
338864
338864
338864
302312
302312
302312
302312
302312
302312
301473
301473
334280
———
878188
878188
302168
302138
331152
338865
338908
338908
301870
301870
330791
330791
330791
326890
326890
330793*
338914*
338913*
808482
338864
338864
338864
338864
302312
302312
302312
302312
302312
346242
801761
346242
341767
341767
326889
326899
330900
338909
338909
11⁄2 in. (38 mm)
Al
Al
Al
Al
Al
326891
326905
330901
338903
338905
* Also order 330899 Washer.
Beckman Coulter Ultracentrifuge
2-9
www.beckmancoultercentrifugation.com
Tools, Accessories,
and Supplies
S
ample preparation and recovery are important steps
in the centrifugation process. To help in these often
time-consuming steps, Beckman Coulter provides a
number of tools and accessories for use in preparing your
samples for centrifugation as well as for recovering the
particles of interest when your separation is complete.
Everything you need can be conveniently ordered from
3
the following listings.
Beckman Coulter Ultracentrifuge
3-1
www.beckmancoultercentrifugation.com
Tools and Supplies
Required Tools
If you’re running capped tubes in the following rotors: Types 90 Ti,
75 Ti, 70.1 Ti, 70 Ti, 60 Ti, 55.2 Ti, 50.2 Ti, 50 Ti, 45 Ti, 42.1, 35, or
MLA-55, you’ll need the following tools. Tools also needed for Types
30 and 21 rotors if capped thickwall tubes are used.
331202 Tool Kit
305075 Cap Vise
If you’re running 337927 caps in the Type 70 Ti rotor, you’ll need:
338841 Tool Kit
If you’re running Quick-Seal tubes, you’ll need:
358312
358313
358314
358315
Tube Topper Kit, 60 Hz (U.S./Canada/Japan)
Tube Topper Kit, 50 Hz (for Europe)
Tube Topper Kit, 50 Hz (for Great Britain)
Tube Topper Kit, 50 Hz (for Australia)
Note: F
orfurtherinformation,refertothespecifictechnicalbulletinfromthe
Technical Publications Department.
305075 Cap Vise
Tools for Fixed-Angle Rotors (see also Tools for Quick-Seal Tubes)
301875 Removal Tool, for capped tubes
841884 Hex Driver, for 5⁄16-in. (8 mm) hex nuts
302460 Nylon Insert Tool, for cap stem filling hole
858122 Socket Adapter, for 858123 Socket below
305075 Tube-Cap Vise
858123 Socket, for tightening red, blue, and black aluminum
caps, 3⁄4-in. (19 mm) hex nut
841883 Hex Driver, for 7⁄16-in. (11 mm) hex nuts
870432 Socket, for tightening titanium caps (cap 341968), 7⁄16-in.
(11 mm) hex nut
3
3-2
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Tools and Supplies
Tools for Fixed-Angle Rotors (cont.)
878133 Removal Tool, for polycarbonate bottles with aluminum
caps
331325 Centering Tool, for replacing overspeed disk
338841 Tool Kit, for Type 70 Ti Rotor when 344367 Tubes and
337927 Caps are used. Includes the following:
335381 Removal Tool, for polycarbonate bottles with Noryl caps
301875
338835
338840
858121
858122
858123
870432
Removal Tool, for capped tubes
Tube Cap Vise
Tube Cap Assembler
Torque Wrench
Socket Adapter
Socket, for 3⁄4 -in. (19 mm) hex nut
Socket, for 7⁄16 -in. (11 mm) hex nut
331202 Tool Kit for capped thin-wall and thick-wall tubes when
used in Types 90 Ti, 75 Ti, 70.1 Ti, 60 Ti, 55.2 Ti, 50.2 Ti,
50 Ti, 45 Ti, 42.1, 35, and MLA-55 Rotors. Also needed
for Types 30 and 21 Rotors if capped thickwall tubes are
used. Includes the following:
301875
858121
858122
858123
870432
3
303419 Removal Tool, for Delrin tube adapters (not for use with
adapters for konical Tubes)
Removal Tool, for capped tubes
Torque Wrench
Socket Adapter
Socket, for 3⁄4 -in. (19 mm) hex nut
Socket, for 7⁄16 -in. (11 mm) hex nut
Beckman Coulter Ultracentrifuge
3-3
www.beckmancoultercentrifugation.com
Tools and Supplies
Tools for Vertical-Tube and NVT Rotors (See also Tools for Quick-Seal Tubes)
Note: V
Ti65,VTi65.1andVTi50rotorsmanufacturedpriorto8/87wereequippedwithpin-styleandsquare-styleplugs,andrequirespecifictoolsmentionedinthebottomof
this section. To update these rotors, purchase a full set of the new hex-style plugs and the appropriate tools.
332688 Rotor Vise, for VTi 65 and VTi 50 rotors
342705 Rotor Vise, for VTi 90, NVT 100*, NVT 90, NVT-65,
VTi 65.1, VTi 65.2, and VAC 50 rotors
*
355588 Hex Plug-wrench Adapter for VTi 50, VC 53, VAC 50
Rotors
356306 Hex Plug-wrench Adapter for VTi 90, VTi 65, VTi 65.1,
VTi 65.2, and MLN-80 Rotors
3
889096 Torque Wrench, 600 in.-lb., forVTi 50, VC 53, VAC 50
Rotors
858121 Torque Wrench, 200 in.-lb. for VTi 90, VTi 65, VTi 65.1,
VTi 65.2, NVT 100, NVT 90, NVT 65.2, VTi 80, TLN-120,
TLN-100, and TLN-80 Rotors
Requires adapter 367084.
Tools for VTi 65, VTI 65.1 and VTi 50 Manufactured before 8/87
340632 Four-hole Plug-wrench Adapter for VTi 50 Rotors
345795 Two-hole Plug-wrench Adapter for VTi 65 Rotors
347372 Hex Plug Adapter for TLN-120, TLN-100, TLV-100
347373 Rotor Vise for TLN-120, TLN-100, TLV-100, TLN-80,
MLA-55
889096 Torque Wrench for VTi 50, VC 50, VAC 50
976959 Torx Wrench Adapter for NVT 100, NVT 90, NVT 65.2,
VTi 90, VTi 65.2, TLV-100, TLN-120
Reminder: These items are supplied with the Rotor Package; order only as
replacements.
Tools for Swinging-Bucket Rotors
001878 Bucket Cap Tool, for SW 60 Ti Rotor
354468
332400
927208
330070
Removal Tool, for konical Tube Adapters
Rotor Stand
Hemostat (6-in., curved)
Hinge Pin Tool, for SW 65 Ti, SW 41 Ti and SW 40 Ti
Rotors
Reminder: These items are supplied with the Rotor Package; order only as
replacements.
Tools for Zonal Rotors (See also Tools for Quick-Seal Tubes)
001884
332618
332848
819247
3-4
Hex Wrench, 5⁄64-in. (1.9 mm), for Support Band
Seal Disassembly Tool
Locating Spacer, for Ti-15, A1-15, Ti-14 Rotors
Lubriplate Grease, 13⁄4-oz. Tube
328917 Tool Kit, for Zonal Rotors.
Includes the following:
332688
Rotor Vise
332690
Spanner Wrench
333763
Tubing Removal Tool
858532
Pliers
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Tools and Supplies
Supplies
306812 Spinkote Lubricant, 2-oz. tube
335148 Vacuum Grease, 1-oz. jar
339379 Brush, for cleaning rotor cavities, 7⁄16 in. to 1 in. (11 to 25 mm)
in diameter; minimum order of three.
339380 Brush, for cleaning rotor cavities, 1 in. to 11⁄2 in. (25 to 38 mm)
in diameter; minimum order of three.
339555 Rotor Cleaning Concentrate, Solution 555, 946-mL
bottle; minimum order of two
339558 Rotor Cleaning Kit (see description on page 2–18)
includes: one 339379 Brush, one 339380 Brush and two
bottles of 339555 Rotor Cleaning Concentrate
347404 Brush, for cleaning rotor cavities, 1⁄4 in. to 1⁄2 in. (6 to 13 mm)
in diameter; minimum order of three.
339587 Master Rotor Logbook
330049 Logbook, for Preparative Ultracentrifuge Instruments
339648 Rotor Record Cards, package of five pads
3
Tools for Quick-Seal Tubes
338765 Removal Tool, for Floating Spacers (except when used in
Swinging Bucket Rotors)
354468 Removal Tool, for konical Tube Adapters used in
Swinging Bucket Rotors
348120 Seal Formers (round-topped) for use with Tube Topper
345395 Ultra-Clear Tube Sealing Oil, 7.5 mL bottle
361668 Removal Tool for Tubes and Spacers (and Floating
Spacers in Swinging Bucket Rotors)
342415 Funnel
Beckman Coulter Ultracentrifuge
3-5
www.beckmancoultercentrifugation.com
Tools and Supplies
Quick-Seal Tube Racks
For Cordless Tube Topper
These autoclavable racks accommodate Quick-Seal Tubes
for use with the Cordless Tube Topper.
348122
348123
348124
348125
349387
349661
356568
Tube Rack (Red), for 1⁄2-in. (13 mm) diameter Tubes
Tube Rack (Green), for 5⁄8-in. (16 mm) diameter Tubes
Tube Rack (Blue), for 1-in. (25 mm) diameter Tubes
Tube Rack (Black), for 11⁄2 -in. (38 mm) diameter Tubes
Tube Rack (Gold), for 7⁄16-in. (11 mm) diameter Tubes
Tube Rack (Orange), for 5⁄16-in. (8 mm) diameter Tubes
Tube Rack (Violet), for 9⁄16-in. (14 mm) diameter Tubes
3
Tools for OptiSeal Tubes
For easy handling of OptiSeal Tubes, specially designed
Tube Racks and Removal Tools are available.
338765
360538
360542
361638
361642
Removal Tool, for 5⁄8-in Floating Spacer (362202)
OptiSeal Tube Rack, for 5⁄8 x 23⁄4-in. (16 x 70 mm) Tubes
OptiSeal Tube Rack, for 1 x 31⁄2-in. (25 x 89 mm) Tubes
OptiSeal Tube Rack, for 1⁄2 x 17⁄8-in. (13 x 48 mm) Tubes
OptiSeal Tube Rack, for 5⁄8 x 23⁄8-in. (16 x 60 mm) Bell top
Tubes
361646 OptiSeal Tube Rack, for 1 x 31⁄4-in. (25 x 77 mm) Bell top
Tubes
361650 OptiSeal Tube Rack, for 1⁄2 x 115⁄16-in. (13 x 33 mm) Tubes
361668 OptiSeal Tube Extraction Tool
3-6
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Tools and Supplies
Cordless Tube Topper
358312
358313
358314
358315
Tube Topper Kit, 60 Hz (U.S./Canada/Japan)
Tube Topper Kit, 50 Hz (for Europe)
Tube Topper Kit, 50 Hz (for Great Britain)
Tube Topper Kit, 50 Hz (for Australia)
Each Kit Contains:
1 each 348117 Heatsink, 2 each 348643 Seal Guide,
8 each 348120 Seal Former (domed top), 1 each Tube
Topper and Charging Unit, 1 each 361668 Tube Extractor.
Replacement Parts
338765
342415
348117
348120
348643
357442
358317
889676
927937
961601
974874
Removal Tool for Plastic Spacers and Floating Spacers
Funnels (two)
Heat Sink
Seal Former (domed top) for Tube Topper
Seal Guide
Flat-top Seal Former for Tube Sealer
Tip
Plastic Box for holding Accessories
Battery
Lamp (screw-type socket)
Lamp (bayonet-type socket)
Beckman Coulter Ultracentrifuge
3
3-7
www.beckmancoultercentrifugation.com
Accessories
Tube Slicer — for Preparative Ultracentrifuge Tubes
303811 Tube Slicer Kit, contains parts listed below.
(Note: Vise not included.)
Replacement Parts
303830
303838
303917
303918
303919
303920
303921
303922
303923
303924
332692
332693
338516
338517
829623
3
Blade Assembly
Blade
Rubber Ring, 1-in. (25 mm), two required
Rubber Ring, 5⁄8 -in. (16 mm), two required
Rubber Ring, 1⁄2 -in. (13 mm), two required
Rubber Ring, 5⁄16 -in. (8 mm), two required
Metal Shim Ring, 1-in. (25 mm), two required
Metal Shim Ring, 5⁄8 -in. (16 mm), two required
Metal Shim Ring, 7⁄16 -in. (13 mm), two required
Metal Shim Ring, 5⁄16 -in. (8 mm), two required
Metal Shim Ring, 9⁄16 -in. (14 mm), two required
Rubber Ring, 9⁄16 -in. (14 mm), two required
Rubber Ring, 7⁄16 -in. (11 mm), two required
Metal Shim Ring, 7⁄16 -in. (11 mm), two required
Thumbscrew
CentriTube Slicer — for Micro Ultracentrifuge Tubes
347960 CentriTube Slicer Kit. Includes Adapter Plates, Blades,
Storage Box, Hardware Kit, Spinkote Lubricant, and
Instruction Manual.
Replacement Parts
306812 Spinkote Lubricant
348299 Blades, coated package of 10
348307 Replacement Hardware Kit (3 thumbscrews,
1 set of blade clips)
354443 Adapter Plate, 13 mm diameter
354444 Adapter Plate, 11 mm diameter
354445 Adapter Plate, 8 mm diameter
354446 Adapter Plate, 7 mm diameter
354471 Blades, uncoated, package of 10
889676 Plastic Storage Box
3-8
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Accessories
Rotor Cleaning Kit
339558 Rotor Cleaning Kit. Contains two 946-mL bottles of
Solution 555 Rotor Cleaning Concentrate, 339379
Rotor Cleaning Brush, and 339380 Rotor Cleaning Brush
Replacement Parts/Supplies
339379 Rotor Cleaning Brush, 5⁄8-in. (16 mm) and 1-in.
(25.4 mm), for Rotor Cavity diameters from 7⁄16-in.
(11 mm) to 1-in. (25.4 mm) (min. order three Brushes)
339380 Rotor Cleaning Brush, 11⁄4-in. (32 mm) and 11⁄2-in. (38
mm), for Rotor Cavity diameters from 1-in. (25.4 mm) to
11⁄2-in. (38 mm) (min. order three Brushes)
339555 Solution 555 Rotor Cleaning Concentrate
(min. order two Bottles)
3
Beckman Coulter Ultracentrifuge
3-9
www.beckmancoultercentrifugation.com
Accessories
Fraction Recovery System
HOLLOW-CONE CAP ASSEMBLY
Lock-ring Collar
339194
Spring
878224
Plug
331593
Thumbscrew
Drain Tube
331598
Hollow-cone
Plug Cap
331593
3
Swivel
Fitting
313336
Bushing
331594
O-ring
807475
Upper Mount
343787
O-ring
863590
Gasket
FLAT-CONE CAP ASSEMBLY
Flat-bottomed
Cap 339192
Tube
Support
Shaft*
Tube
Mounting
Pad
343785
Plug
885086
Guide Post
343781
Lower Mount
Thumbscrew
343795
Swivel Fitting
313336
Gasket
339191
Teflon Tubing
Needle
Assembly
343786
Seal, Cushion
331601
Ringstand
Two types of systems are available: one with a
flatbottomed cap, and those with hollow-cone caps.
Using the flat-bottomed cap, sample is removed through a
puncture hole in the bottom of the tube. This cap fits any
tube diameter and must be used to fractionate QuickSeal tubes. The four hollow-cone cap systems are used
for sample recovery from tubes that cannot be punctured
or for recovery from the tops or bottoms of puncturable
tubes. Each hollow-cone system is for a single tube
diameter.
System Part Numbers
342023 Fraction Recovery System with Hollow-cone
Cap for 1⁄2 -in. Tubes.
342024 Fraction Recovery System with Hollow-cone
Cap for 5⁄8 -in. Tubes.
342025 Fraction Recovery System with Hollow-cone
Cap for 7⁄16 -in. Tubes.
342026 Fraction Recovery System with Hollow-cone
Cap for 1-in. Tubes.
343890 Fraction Recovery System with flat-bottomed
Cap for puncturing all Tubes, including
Quick-Seal Tubes*.
Hollow-Cone Caps
331562
331563
331564
331565
331566
331586
331587
331588
331589
331591
334310
334312
Hollow-cone Cap for 1-in. diameter Tubes
Hollow-cone Cap for 11⁄4 -in. diameter Tubes
Hollow-cone Cap for 5⁄8 -in. diameter Tubes
Hollow-cone Cap for 1⁄2 -in. diameter Tubes
Hollow-cone Cap for 9⁄16 -in. diameter Tubes
Gasket for 331566 Cap
Gasket for 331565 Cap
Gasket for 331564 Cap
Gasket for 331563 Cap
Gasket for 331562 Cap
Hollow-cone Cap for 7⁄16 -in. diameter Tubes
Gasket for 334310 Cap
Replacement Parts for Discontinued 339187 Universal Fraction Recovery System
331584 Piercing Screw
343786 Needle Assembly
343804 Retrofit Kit, includes lower mount (shown
left) for lever puncturing to 343890
819838 Setscrew with plastic tip
* 346105 Tube Support Shaft, 2-in., 346106 Tube Support Shaft,
3-in., both included in 343890.
3-10
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Reference
Guide to Centrifuge Selection
Centrifugation is a basic separation technique that is utilized at multiple
stages in the study of sample components. Flexible rotor and adapter
systems for Beckman Coulter centrifuges allow them to be used across
multiple application areas. To help you select the most appropriate
centrifuge for your work, the following charts provide brief descriptions
of the kinds of separations typically achieved using various centrifuges.
These charts list frequent separation requirements for each sample type
and identify the centrifuges that are typically used to meet those requirements.
In addition to the separation and isolation of sample particles, centrifugation is increasingly being used as an analytical technique for the study
of macromolecular interactions and the determination of molecular
weights. Instruments for these applications are also listed below.
Quick-Reference Guide to Centrifuge Selection
Avanti Series
Microfuge
Allegra Series
J6 Series
Centrifuges Typically Used
Optima MAX Series
Airfuge
Specific Application
Optima X Series
Materials to be Isolated
Preparative Centrifugation
Ammonium sulfate precipitates
Sucrose/glycerol gradient isolation
Centrifugal filtration
•
Fractionation by flotation
Density gradient fractionation
Fractionation by precipitation
Chylomicron removal
•
Sucrose gradient isolation
•
•
Pelleting
Sucrose gradient isolation
Microsomal membrane fractionation
•
•
•
•
Mitochondria
Pelleting
Sucrose gradient isolation
•
•
Nuclei
Pelleting
•
•
Membranes
Pelleting
Sucrose/Percoll gradient fractionation
Binding studies
•
•
•
•
•
•
Ribosomes/Polysomes
Pelleting
Size fractionation in sucrose gradients
•
•
•
Cytosol
Clarification
•
•
•
Proteins
4
Lipoproteins
Subcellular Fraction
Chromatin/Nucleosomes
Microsomes
4-2
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Reference
Quick-Reference Guide to Centrifuge Selection (cont.)
Specific Application
Optima X Series
Optima MAX Series
Airfuge
J6 Series
Allegra Series
Microfuge
Avanti Series
Materials to be Isolated
Preparative Centrifugation
Lysates/Homogenates
•
•
Clearing debris and large particles
•
•
•
•
Nucleic Acids
DNA
RNA
Cells
Viruses
•
Alcohol precipitation
Phenol/CHC13 extraction
Plasmid purification in CsCl
Size fractionation in sucrose gradients
Minipreps in 96-well plates
Spin columns
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Isolation of mononuclear cells on
Ficoll-Hypaque
Pelleting bacteria
Pelleting mammalian cells
Elutriation of viable cells
Other density gradient separations
Beckman Coulter Ultracentrifuge
•
•
Phenol/CHC13 extraction
Alcohol precipitation
Lithium precipitation
Pelleting in CsCl
CSTFA density gradient
Pelleting
PEG precipitates
Density gradient isolations
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
4
•
•
4-3
www.beckmancoultercentrifugation.com
Reference
How to Choose a Rotor
When choosing a rotor, there are several factors to consider:
your sample volume and number of individual samples; the
number of components in each sample; the level of purity your
research requires; and how quickly you wish to achieve the
separation.
The Importance of k Factor
Obviously, separation is affected by maximum speed and
maximum radius which together determine maximum g-force.
However, particle pathlength also affects separation time. A
simple measure of overall rotor efficiency which incorporates
both g-force and particle pathlength is the k Factor. Generally
speaking, the lower the k Factor, the shorter the run time. This
makes the k Factor one of the most important considerations
when selecting a rotor. The chart on 4-5 compares k Factors
among the four basic rotor types. (Refer to page 4-12 for
useful formulas relating to k Factors.)
4
Rotor Material
Beckman Coulter ultracentrifuge rotors are made of either
aluminum, or titanium.
• Aluminum rotors are relatively light and easy to handle,
but less durable and slightly more susceptible to corrosion
than titanium rotors. Beckman Coulter still offers some
aluminum rotors for customers who own older ultracentrifuges, the primary instruments for which these rotors were
originally designed.
• Titanium rotors are generally stronger and more resistant
to corrosion, making them the logical choice for rapid
separation at high speeds or when corrosive chemicals
will be used.
Vertical-Tube (VT) Rotors
These rotors are often used for isopycnic and rate zonal
separations when run-time reduction is important. Since
vertical tube rotors hold sample tubes parallel to the axis of
rotation, particle pathlengths are limited to the diameter of the
tube — a short pathlength that results in fast run times.
NVT Near-Vertical Tube Rotors
NVT Near-Vertical Tube Rotors, a patented Beckman Coulter
innovation, were designed specifically for density gradient
separations. Their narrow angle of less than 10° is calculated
using a proprietary algorithm which determines the optimal
angle for each rotor, taking into consideration specific tube
geometries. These angles result in significantly reduced run
times compared with conventional Fixed-Angle Rotors, while
positioning pelleted and floated components at the ends of the
tubes, away from the bands of interest.
Continuous-Flow/Zonal Rotors
These rotors are capable of processing large sample volumes
in their cores. This large-scale processing ability is clearly
evident in Continuous-Flow Rotors, which can process a
typical 10-liter sample in about four hours, instead of the
12-24 hours required by conventional methods. Zonal Rotors
have similar utility in large-scale density gradient separations.
Swinging-Bucket (SW) Rotors
Generally used when maximum resolution of sample zones
is needed, as in rate zonal studies. Because tubes are held in
a horizontal position while spinning, the pathlength is the
full length of the tube, which results in longer run times than
with other rotor types. These long run times are offset by
excellent resolution of sample bands in rate zonal separations.
Swinging-Bucket Rotors are also the best choice when a
compact pellet is needed, as when pelleting RNA through a
cesium chloride cushion.
Fixed-Angle (FA) Rotors
These rotors provide faster run times than SW rotors at the
expense of some resolution in rate zonal studies. They are
most useful for pelleting and for isopycnic banding of DNA,
where a shallow density gradient and reorientation combine
to increase both the width of sample bands and the distance
between them, making band extraction easier.
4-4
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Reference
Particle Separation in Swinging-Bucket, Fixed-Angle, Near-Vertical Tube, and Vertical-Tube Rotors
At Speed in Rotor
At Rest in Rotor
At Rest Outside Rotor
Swinging-Bucket Rotors
Typical Example: SW 60 Ti
Pathlength: 57.2 mm
k Factor: 45
rmin
rmax
Pathlength
Fixed-Angle Rotors
Typical Example: Type 90 Ti
Pathlength: 42.3 mm
k Factor: 25
rmax
rmin
Pathlength
4
Near-Vertical Tube Rotors
Typical Example: NVT 90
Pathlength: 18.7 mm
k Factor: 10
rmin
rmax
Pathlength
Vertical-Tube Rotors
Typical Example: VTi 90
Pathlength: 13.2 mm
k Factor: 6
rmin
rmax
Pathlength
Dark orange represents pelleted material, light orange depicts floating components, and bands are indicated by black lines.
Beckman Coulter Ultracentrifuge
4-5
www.beckmancoultercentrifugation.com
n
n
n
n
n
n
n
n
n
Largest Volume
n
Largest Number of Samples
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Fastest Pelleting
n
n
n
n
n
Largest Volume Rate-Zonal Separation
Fastest Rate-Zonal Separation
Separation of Viruses
and Viral Particles
n
n
n
n
n
Fastest Rate-Zonal Separation
n
n
n
Fastest Separation
n
n
n
n
n
Largest Volume for Pelleting
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Largest Number for Differential Flotation
n
n
n
n
Ti-15
VTi 50
VTi 65.2
VTi 65.1
VTi 90
NVT 65.2
NVT 90
NVT 65
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Largest Volume
n
n
n
Greatest Interband Space
Fastest Separation
n
n
n
Largest Volume for Differential Flotation
Isopycnic Separation
of Plasmid DNA
n
n
n
n
Fastest Differential Flotation
Fastest Separation
n
n
Greatest Interband Distance
Pelleting RNA Through
a CsCI Gradient
n
n
n
Largest Volume
Largest Number of Samples
Separation of
Lipoproteins
n
n
Largest Volume Rate-Zonal Separation
Rate-Zonal Separation
of Proteins in Sucrose
Gradient
n
n
n
Fastest Pelleting
n
n
TLS-55
Largest Volume for Pelleting
n
n
n
MLA-50
Separation of
Subcellular Particles
TLA-55
Specific Application
MLA-55
Bioseparation
n
MLA-80
Tabletop Ultracentrifuge
Rotor Selection by Application*
n
TLA-100
n
TLA-100.3
n
TLA-110
n
TLA-120.1
n
TLA-120.2
n
MLA-130
n
MLA-150
4
Largest Volume
NVT 100
SW 28
n
Fastest Separation
n
n
n
Fastest Separation
Greatest Interband Distance
n
n
n
Fastest Density Gradient Separation
n
n
n
Greatest Interband Space
Isopycnic
Separation of
Plasmid DNA
n
n
n
Pelleting RNA
Through a
Largest Volume
CsCl
Gradient
n
n
n
n
n
n
Largest Number for Differential Flotation
Largest Volume for Differential Flotation
n
n
n
n
Greatest Interband Distance
n
n
n
n
n
MLN-80
Separation of
Lipoproteins
Fastest Differential Flotation
n
n
n
TLN-100
Fastest Separation
n
n
Fastest Rate-Zonal Separation
Rate-Zonal
Separation
of Proteins
in Sucrose
Gradient
n
n
TLN-120
Largest Volume for Pelleting
Separation
of Virus and
Largest Volume Rate-Zonal Separation
Viral Particles
SW 28.1
n
n
MLS-50
Fastest Rate-Zonal Separation
SW 32 Ti
SW 32.1 Ti
SW 40 Ti
SW 41 Ti
SW 55 Ti
SW 60 Ti
n
Largest Volume Rate-Zonal Separation
CF-32 Ti
n
Type 19
n
Type 25
n
Type 45 Ti
Type 50.2 Ti
n
Type 42.2 Ti
Type 70 Ti
n
Largest Volume for Pelleting
Type 50.4 Ti
Type 70.1 Ti
Separation of
Subcellular
Particles
Type 90 Ti
Floor Model Ultracentrifuge
Rotor Selection by
Application*
Type 100 Ti
Reference
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
Greatest Interband Distance
n
n
n
n
Largest Volume
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
*Selected rotor has the capability (RCF, volume, labware) to accommodate the application, but may not be the most optimal/efficient choice for the specific application.
4-6
Beckman Coulter Ultracentrifuge
n
www.beckmancoultercentrifugation.com
Reference
Gradient Materials
There is no ideal all-purpose gradient material. Sucrose is
used for most rate separations and cesium chloride is often
used for isopycnic separations. The basic requirement is that
the gradient permits the desired type of separation. Additional
considerations in selecting a gradient material include the
following:
• Its density range should be sufficient to permit separation
of the particles of interest by the chosen density gradient
technique, without overstressing the rotor.
• It should not affect the biological activity of the sample.
• It should be neither hyperosmotic nor hypoosmotic when
the sample is composed of sensitive organelles.
• It should not interfere with the assay technique.
• It should be removable from the purified product.
• It should not absorb in the ultraviolet or visible range.
• It should be inexpensive and readily available; more
expensive materials should be recoverable for reuse.
4
• It should be sterilizable.
• It should not be corrosive to the rotor, particularly for zonal
or continuous-flow operation.
• It should not be flammable or toxic to the extent that its
aerosols could be hazardous.
The following tables are provided as a reference for
information on commonly used gradient materials.
Beckman Coulter Ultracentrifuge
4-7
www.beckmancoultercentrifugation.com
Reference
Commonly Used Gradient Materials with Their Solvents
Materials
Solvent
Maximum
Density
at 20°C
Sucrose (66%)
Sucrose (65%)
Silica sols
Diodon
Glycerol
H2O
D2O
H2O
H2O
H2O
1.32
1.37
1.30
1.37
1.26
Cesium chloride
H2O
D2O
H2O
H2O
H2O
H2O
1.91
1.98
2.10
2.00
1.49
1.85
Lithium bromide
H2O
H2O
H2O
D2O
H2O
D2O
H2O
1.63
1.41
1.57
1.63
1.32
1.40
1.83
Lithium chloride
Albumin
Sorbitol
Ficoll
Metrizamide
D2O
H2O
H2O
H2O
H2O
1.33
1.35
1.39
1.17
1.46
Cesium formate
Cesium acetate
Rubidium chloride
Rubidium formate
Rubidium bromide
Potassium acetate
Potassium formate
Sodium formate
4
4-8
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Reference
Density, Refractive Index, and Concentration Data—Cesium Chloride at 25°C
Molecular Weight = 168.37
Density
(g/cm3)*
Refractive
Index, ηD
% by
Weight
mg/ml of
Solution**
Molarity
Density
(g/cm3)*
Refractive
Index, ηD
% by
Weight
mg/ml of
Solution**
Molarity
1.0047
1.0125
1.0204
1.0284
1.0365
1.3333
1.3340
1.3348
1.3356
1.3364
1
2
3
4
5
10.0
20.2
30.6
41.1
51.8
0.056
0.119
0.182
0.244
0.308
1.336
1.3496
1.363
1.377
1.391
1.3657
1.3670
1.3683
1.3696
1.3709
34
35
36
37
38
454.2
472.4
490.7
509.5
528.6
2.698
2.806
2.914
3.026
3.140
1.0447
1.0531
1.0615
1.0700
1.0788
1.3372
1.3380
1.3388
1.3397
1.3405
6
7
8
9
10
62.8
73.7
84.9
96.3
107.9
0.373
0.438
0.504
0.572
0.641
1.406
1.4196
1.435
1.450
1.465
1.3722
1.3735
1.3750
1.3764
1.3778
39
40
41
42
43
548.3
567.8
588.4
609.0
630.0
3.257
3.372
3.495
3.617
3.742
1.0877
1.0967
1.1059
1.1151
1.1245
1.3414
1.3423
1.3432
1.3441
1.3450
11
12
13
14
15
119.6
131.6
143.8
156.1
168.7
0.710
0.782
0.854
0.927
1.002
1.481
1.4969
1.513
1.529
1.546
1.3792
1.3807
1.3822
1.3837
1.3852
44
45
46
47
48
651.6
673.6
696.0
718.6
742.1
3.870
4.001
4.134
4.268
4.408
1.1340
1.1437
1.1536
1.1637
1.1739
1.3459
1.3468
1.3478
1.3488
1.3498
16
17
18
19
20
181.4
194.4
207.6
221.1
234.8
1.077
1.155
1.233
1.313
1.395
1.564
1.5825
1.601
1.619
1.638
1.3868
1.3885
1.3903
1.3920
1.3937
49
50
51
52
53
766.4
791.3
816.5
841.9
868.1
4.552
4.700
4.849
5.000
5.156
1.1843
1.1948
1.2055
1.2164
1.2275
1.3508
1.3518
1.3529
1.3539
1.3550
21
22
23
24
25
248.7
262.9
277.3
291.9
306.9
1.477
1.561
1.647
1.734
1.823
1.658
1.6778
1.699
1.720
1.741
1.3955
1.3973
1.3992
1.4012
1.4032
54
55
56
57
58
895.3
922.8
951.4
980.4
1009.8
5.317
5.481
5.651
5.823
5.998
1.2387
1.2502
1.2619
1.2738
1.2858
1.3561
1.3572
1.3584
1.3596
1.3607
26
27
28
29
30
322.1
337.6
353.3
369.4
385.7
1.913
2.005
2.098
2.194
2.291
1.763
1.7846
1.808
1.831
1.856
1.4052
1.4072
1.4093
1.4115
1.4137
59
60
61
62
63
1040.2
1070.8
1102.9
1135.8
1167.3
6.178
6.360
6.550
6.746
6.945
1.298
1.311
1.324
1.3619
1.3631
1.3644
31
32
33
402.4
419.5
436.9
2.390
2.492
2.595
1.880
1.9052
1.4160
1.4183
64
65
1203.2
1238.4
7.146
7.355
4
Density data are from International Critical Tables.
** Computed from the relationship p25 = 10.2402 hD25—12.6483 for densities between 1.00 and 1.38, and p25 = /0.8601 hD25—13.4974 for densities above 1.37
(Bruner and Vinograd, 1965).
** Divide by 10.0 to obtain % w/v.
Beckman Coulter Ultracentrifuge
4-9
www.beckmancoultercentrifugation.com
Reference
Density, Refractive Index, and Concentration Data – Sucrose at 20°C, Molecular Weight = 342.3
4
Density
(g/cm3)
Refractive
Index, ηD
% by
Weight
mg/ml of
Solution*
Molarity
Density
(g/cm3)
Refractive
Index, ηD
% by
Weight
mg/ml of
Solution*
Molarity
0.9982
1.0021
1.0060
1.0099
1.0139
1.3330
1.3344
1.3359
1.3374
1.3388
0
1
2
3
4
10.0
20.1
30.3
40.6
0.029
0.059
0.089
0.119
1.1463
1.1513
1.1562
1.1612
1.1663
1.3883
1.3902
1.3920
1.3939
1.3958
34
35
36
37
38
389.7
403.0
416.2
429.6
443.2
1.138
1.177
1.216
1.255
1.295
1.0179
1.0219
1.0259
1.0299
1.0340
1.3403
1.3418
1.3433
1.3448
1.3464
5
6
7
8
9
50.9
61.3
71.8
82.4
93.1
0.149
0.179
0.210
0.211
0.272
1.1713
1.1764
1.1816
1.1868
1.1920
1.3978
1.3997
1.4016
1.4036
1.4056
39
40
41
42
43
456.8
470.6
484.5
498.5
512.6
1.334
1.375
1.415
1.456
1.498
1.0381
1.0423
1.0465
1.0507
1.0549
1.3479
1.3494
1.3510
1.3526
1.3541
10
11
12
13
14
103.8
114.7
125.6
136.6
147.7
0.303
0.335
0.367
0.399
0.431
1.1972
1.2025
1.2079
1.2132
1.2186
1.4076
1.4096
1.4117
1.4137
1.4158
44
45
46
47
48
526.8
541.1
555.6
570.2
584.9
1.539
1.581
1.623
1.666
1.709
1.0592
1.0635
1.0678
1.0721
1.0765
1.3557
1.3573
1.3590
1.3606
1.3622
15
16
17
18
19
158.9
170.2
181.5
193.0
204.5
0.464
0.497
0.530
0.564
0.597
1.2241
1.2296
1.2351
1.2406
1.2462
1.4179
1.4200
1.4221
1.4242
1.4264
49
50
51
52
53
599.8
614.8
629.9
645.1
660.5
1.752
1.796
1.840
1.885
1.930
1.0810
1.0854
1.0899
1.0944
1.0990
1.3639
1.3655
1.3672
1.3689
1.3706
20
21
22
23
24
216.2
227.9
239.8
251.7
263.8
0.632
0.666
0.701
0.735
0.771
1.2519
1.2575
1.2632
1.2690
1.2748
1.4285
1.5307
1.4329
1.4351
1.4373
54
55
56
57
58
676.0
691.6
707.4
723.3
739.4
1.975
2.020
2.067
2.113
2.160
1.1036
1.1082
1.1128
1.1175
1.1222
1.3723
1.3740
1.3758
1.3775
1.3793
25
26
27
28
29
275.9
288.1
300.5
312.9
325.4
0.806
0.842
0.878
0.914
0.951
1.2806
1.2865
1.2924
1.2983
1.3043
1.4396
1.4418
1.4441
1.4464
1.4486
59
60
61
62
63
755.6
771.9
788.3
804.9
821.7
2.207
2.255
2.303
2.351
2.401
1.1270
1.1318
1.1366
1.1415
1.3811
1.3829
1.3847
1.3865
30
31
32
33
338.1
350.9
363.7
376.7
0.988
1.025
1.063
1.100
1.3103
1.3163
1.3224
1.3286
1.4509
1.4532
1.4558
1.4581
64
65
66
67
838.6
855.6
872.8
890.2
2.450
2.500
2.550
2.864
Density and refractive index data are from the International Critical Tables.
** Divide by 10.0 to obtain % w/v.
4-10
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Reference
Density Conversion for Cesium and Rubidium Salts at 20°C
Sucrose at 20°C, Molecular Weight = 342 .3
% w/w
CsCl
CsBr
Csl
Cs2SO4
CsNO3
RbCl
RbBr
Rbl
Rb2SO4
RbNO3
1
2
4
6
8
1.00593
1.01374
1.02969
1.04609
1.06297
1.00612
1.01412
1.03048
1.04734
1.06472
1.00608
1.01402
1.03029
1.04707
1.06438
1.0061
1.0144
1.0316
1.0494
1.0676
1.00566
1.01319
1.02859
1.04443
1.06072
1.00561
1.01307
1.02825
1.04379
1.05917
1.00593
1.01372
1.02965
1.04604
1.06291
1.00591
1.01370
1.02963
1.04604
1.06296
1.0066
1.0150
1.0322
1.0499
1.0680
1.0053
1.0125
1.0272
1.0422
1.0575
10
12
14
16
18
1.08036
1.09828
1.11676
1.13582
1.15549
1.08265
1.10116
1.12029
1.14007
1.16053
1.08225
1.10071
1.11979
1.13953
1.15996
1.0870
1.1071
1.1275
1.1484
1.1696
1.07745
1.09463
1.11227
1.07604
1.09281
1.11004
1.12775
1.14596
1.08028
1.09817
1.11661
1.13563
1.15526
1.08041
1.09842
1.11701
1.13621
1.15605
1.0864
1.1052
1.1246
1.1446
1.1652
1.0731
1.0892
1.1057
1.1227
1.1401
20
22
24
26
28
1.17580
1.19679
1.21849
1.24093
1.26414
1.18107
1.20362
1.22634
1.24990
1.27435
1.18112
1.20305
1.22580
1.24942
1.27395
1.1913
1.2137
1.2375
1.2643
1.16469
1.18396
1.20379
1.22421
1.24524
1.17554
1.19650
1.21817
1.24059
1.26380
1.17657
1.19781
1.21980
1.24257
1.26616
1.1864
1.2083
1.2309
1.2542
1.2782
1.1580
1.1763
1.1952
1.2146
1.2346
30
35
40
45
50
1.28817
1.35218
1.42245
1.49993
1.58575
1.29973
1.36764
1.44275
1.52626
1.61970
1.29944
1.36776
1.44354
1.52803
1.62278
1.26691
1.32407
1.38599
1.45330
1.52675
1.28784
1.35191
1.42233
1.50010
1.58639
1.29061
1.35598
1.42806
1.50792
1.59691
1.3028
1.3281
1.2552
1.2764
55
60
65
1.68137
1.78859
1.90966
1.72492
1.68254
1.69667
1.80924
1 93722
Beckman Coulter Ultracentrifuge
4
4-11
www.beckmancoultercentrifugation.com
Reference
Useful Formulas
k Factor
To determine k Factor
To determine pelleting time (t)
k=
t=
To relate pelleting time between rotors
To adjust k Factor for runs less than maximum
rotor speed
ln (rmax/rmin)
ω2
k
s
×
10-13
3600
OR k =
2.53 × 105 ln (rmax/rmin)
(rpm/1000)2
where s = sedimentation coefficient in Svedbergs
k
k1
= t2
t1
2
kadj = k
rated speed of rotor
(maximum
)
actual run speed
2
To relate relative centrifugal force (RCF) to speed (rpm):
RCFmax = 1.12 rmax
4
rpm
( 1000
)
2
OR rpm = 103
RCF
1.12 rmax
To relate the sedimentation coefficient (s) to rotational speed:
s=
Svedberg unit (S) equivalent:
1
dr
×
ω2r
dt
S = 10-13 seconds
Reduced run speed for dense solutions:
reduced run speed = max rated speed of rotor × A/B
where A = max. permissible density of rotor tube contents, and
B = actual density of the tubes to be centrifuged
4-12
Beckman Coulter Ultracentrifuge
www.beckmancoultercentrifugation.com
Support Services
5
Beckman Coulter Ultracentrifuge
5-1
www.beckmancoultercentrifugation.com
Support Services
Field Service Support
Most of us appreciate the need for careful design, handling,
and maintenance of certain kinds of laboratory equipment—
the optics of a spectrophotometer, for example. It may not
be obvious, however, that anything as substantial looking as
a centrifuge rotor must be properly maintained during use in
order for it to fulfill its life expectancy.
If you consider that, during rotation, an Ultracentrifuge
Rotor may experience more than 1,000,000 times the force of
gravity, it becomes apparent that seemingly minor flaws will
assume much greater significance at these g-forces. In effect,
one gram will “weigh” 1,000 kilograms, and a tiny flaw in a
critical part may generate stresses greater than the rotor was
designed to withstand.
We at Beckman Coulter maintain stringent quality
standards and rigorously test every rotor design. Specific
instructions for care and maintenance are included in the
rotor manual that accompanies each rotor. And our Field
Rotor Inspection Program is available at no charge to all
users of Beckman Coulter Ultracentrifuges and High-Speed
Centrifuges.
5
Field Rotor Inspection Program. What Is It?
Our Field Rotor Inspection Program (FRIP) has two
purposes: to prevent premature rotor failures by detection of
stress corrosion, metal fatigue, wear or damage to anodized
coatings; and to instruct laboratory personnel in the proper
care of rotors.
Contact your local Beckman Coulter Service Office for
details on the Field Rotor Inspection Program. Using
nondestructive methods (fiber optics borescopy and dyepenetrant analysis), they may find signs of corrosion or other
damage. If so, they will recommend repair or replacement,
and a potentially costly failure may be prevented.
To give a fuller understanding of rotors, a comprehensive
slide presentation is also offered. Centrifuge users are
informed about maintenance procedures, rotor damage,
and its significance. The stress corrosion of aluminum rotors,
in particular, can be greatly reduced by good laboratory
practice.
For more information about the Field Rotor Inspection
Program (FRIP), please go to: www.beckmanbuilt.com.
Sometimes rotor corrosion is so bad it can be seen by the
naked eye, while at other times it is not so apparent. If you
have doubt about the condition of a single Beckman Coulter
5-2
rotor, you may return it to the factory where it will be
inspected free of charge by our metallurgists. The rotor
will be examined by a variety of nondestructive methods,
including ultrasound which is capable of detecting internal
flaws. Before shipping the rotor, you must contact the
nearest Beckman Coulter Sales and Service office for specific
instructions. A written statement must accompany each rotor,
indicating that it is safe to handle, (i.e. free of any pathogenic
or radioactive contamination).
Rotor Safety Seminars
To help you get the most from your Beckman Coulter rotors,
Rotor Safety Seminars are frequently presented by our Field
Service engineers. Seminars are designed to remind centrifuge
users about the importance of proper rotor care and maintenance.
When you attend a Beckman Coulter Rotor Safety
Seminar, you will have an opportunity to ask questions that
are specific to your particular situation, and you will learn:
•
What causes corrosion in a rotor, where it starts, what it
does to the rotor, and how to avoid it.
•
The proper way to clean and store rotors.
•
How to get longer life from your rotor.
On-Site Rotor Inspection and Maintenance
Specially trained Beckman Coulter Field Service engineers
are available at your request for on-site inspection and minor
rotor maintenance. Rotors that are free of biological and
radiological contamination can be brought to these sessions
for immediate inspection so you can continue operating your
centrifuge with confidence in the condition of your rotors.
A Comprehensive Approach to Centrifuge System Care
Throughout the world, Beckman Coulter Field Service
engineers are on hand to provide the support you need.
So you can operate your centrifuges worry-free, a number
of programs are in place that cover preventive maintenance
of your system, including parts, labor, drive systems, and
rotors. It all adds up to a comprehensive approach to
maximizing your system investment.
Contact your local Beckman Coulter Sales and Service
office to find out the specifics about the program available
in your area.
Beckman Coulter Ultracentrifuge
HIGH-PERFORMANCE &
HIGH-CAPACITY CENTRIFUGES
INGENUITY MEETS PRACTICALITY.
www.beckmancoultercentrifugation.com
S
ince the introduction of the first commercial
Rotors with the BioCertified symbol and have been
ultracentrifuge in 1947 — the classic Beckman
tested to demonstrate containment of aerosols under normal
Model L — Beckman Coulter has been at the
operating conditions of the associated Beckman Coulter
forefront of centrifuge innovation. Although the physics
of this basic separation technique never change, Beckman
centrifuge when used and maintained as instructed.
Following the centrifuge descriptions, listings of their
Coulter continually designs new and innovative rotors and
rotors are included with information on speed and g-force
accessories, and develops advanced methods that allow
capability. Also included is information on tubes and bottles
the forces of centrifugation to be applied in new ways.
that can be used and the adapters they require.
This centrifuge product selection guide is designed
Tubes and bottles are cross-referenced in a separate
to help you determine the most efficient centrifuge tools
section that provides details on tube materials, chemical
for your laboratory.
compatibility, tube designs, and tube closure options.
Each section begins with a brief description of instruments
A reference section at the back of the guide includes
that Beckman Coulter offers within that centrifuge category.
quick-reference charts on instrument and tube selection, as
Because biocontainment is a major concern in today’s
well as frequently used formulas.
laboratories, Beckman Coulter provides a number of options
that address this issue. Special BioSafe instruments and
accessories are available across our centrifuge product line
and are identified with these icons:
BioSafe and BioSafety are terms intended to
describe the enhanced biocontainment features of
our products.
BioCertified is a term used to describe our products
which have been tested and validated to demonstrate
containment of microbiological aerosols by an
independent, third-party facility (Health Protection
Agency, Porton Down, UK or USAMRIID, Ft.
Detrick, MD, USA). Improper use or maintenance
may affect seal integrity and, thus, containment.
BioEnhanced is a term intended to describe
our products' enhanced level of biocontainment
by design.
Beckman Coulter High Performance Centrifuges
i
www.beckmancoultercentrifugation.com
Table of Contents
High Performance and High Capacity Centrifugation
High Performance Centrifuges
Avanti J-26S High Performance Centrifuges......................................................................................................................... 1-2
Avanti JXN-26 High Performance Centrifuge....................................................................................................................... 1-4
Avanti J-E High Performance Centrifuge .............................................................................................................................. 1-6
Avanti J-30I High Performance Centrifuge ........................................................................................................................... 1-7
High Capacity Centrifuges
J6-MI High Capacity Centrifuge ........................................................................................................................................... 1-8
Avanti J-HC Centrifuge ......................................................................................................................................................... 1-9
Tubes and Bottles
Tubes and Bottles for Every Application ...................................................................................................................................... 2-1
Tube Selection Considerations
Compatibility of Tube Material with Solvents and Samples ................................................................................................. 2-2
Gradient Formation and Fractionation................................................................................................................................... 2-2
High Temperature Centrifugation .......................................................................................................................................... 2-2
Tube Sizes ..................................................................................................................................................................................... 2-2
Tube Cleaning, Sterilization and Reuse ........................................................................................................................................ 2-2
Tube Closures ............................................................................................................................................................................... 2-3
A Quick Reference Chart to Tube Materials and Their Properties ............................................................................................... 2-3
General Filling and Sealing Requirements ................................................................................................................................... 2-4
High Performance and High Capacity Bottles .............................................................................................................................. 2-5
Adapters for Non-Beckman Coulter Tubes/Bottles ...................................................................................................................... 2-6
Adapters and Sleeves for Glass Tubes in Beckman Coulter High Performance Rotors ............................................................... 2-6
Tubes and Bottles (Listed by Volume) .......................................................................................................................................... 2-7
HarvestLine System Liners ......................................................................................................................................................... 2-10
Tools and Supplies .......................................................................................................................................................................2-11
Reference
Guide to Centrifuge Selection....................................................................................................................................................... 3-2
Useful Formulas ............................................................................................................................................................................ 3-4
Support Services
Field Rotor Inspection Program and On-Site Support .................................................................................................................. 4-2
Beckman Coulter High Performance Centrifuges
iii
High-Performance & High-Capacity Centrifugation
The power to accomplish more.
In the footsteps of our founder, Arnold O. Beckman, Ph.D., we at Beckman Coulter remain dedicated
to your resourcefulness. In fact, we help create the world’s most productive and efficient laboratories
by providing the finest centrifuges available.
And with our high-performance and high-capacity solutions, we’ve set an all-new standard for
enhanced power, speed and flexibility. As a result, you can achieve more throughput and greater
control over your lab’s workflow.
Beckman Coulter has earned the sobriquet of gold standard from our customers around the globe.
And the quality built into our high-performance and high-capacity centrifuges is a shining example of
just how far innovation can lead.
www.beckmancoultercentrifugation.com
High Performance
and High Capacity
Centrifugation
High Performance Centrifuges
Avanti JXN-26 High Performance Centrifuge..............1-2
Avanti J-26S High Performance Centrifuges................1-4
Avanti J-E High Performance Centrifuge .....................1-6
Avanti J-30I High Performance Centrifuge ..................1-7
High Capacity Centrifuges
J6-MI High Capacity Centrifuge ..................................1-8
Avanti J-HC Centrifuge ................................................1-9
High Performance Centrifuge/Rotor Compatibility Chart .1-10
High Performance and High Capacity Rotor Summary ....1-12
Rotor Selection by Application ..........................................1-15
Beckman Coulter High Performance Centrifuges
1
Fixed-Angle Rotors
JA-30.50 Ti .................................................................1-16
JA-25.50......................................................................1-17
JA-25.15......................................................................1-18
JA-21...........................................................................1-19
JA-20.1........................................................................1-20
JA-20...........................................................................1-21
JA-18.1........................................................................1-22
JA-18...........................................................................1-23
JA-17...........................................................................1-24
J-LITE JLA-16.250.....................................................1-25
JA-14.50......................................................................1-26
JA-14...........................................................................1-27
JA-12...........................................................................1-28
J-LITE JLA-10.500 .....................................................1-29
JA-10...........................................................................1-30
J-LITE JLA-9.1000 .....................................................1-31
J-LITE JLA-8.1000.....................................................1-33
Swinging Bucket Rotors
JS-24.38 ......................................................................1-35
JS-24.15 ......................................................................1-36
JS-13.1 ........................................................................1-37
JS-7.5 ..........................................................................1-38
JS-5.9 ..........................................................................1-40
JS-5.3 ..........................................................................1-41
JS-5.2 ..........................................................................1-43
JS-5.0 ..........................................................................1-46
JS-4.3 ..........................................................................1-47
JS-4.2 ..........................................................................1-50
JS-4.2A........................................................................1-52
JS-4.2SM .....................................................................1-54
JS-4.2SMA..................................................................1-55
JS-4.0 ..........................................................................1-56
JS-3.0 ..........................................................................1-59
Zonal and Continuous Flow Rotors
JCF-Z ..........................................................................1-62
Elutriation Rotors
JE-5.0 ..........................................................................1-64
1-1
www.beckmancoultercentrifugation.com
High Performance Centrifugation
1
Avanti JXN-26 High Performance Centrifuge
The versatility you need. The performance you expect.
The Avanti JXN-26 is a refrigerated centrifuge that generates
centrifugal forces required for a wide variety of applications.
It achieves up to 26,000 rpm/81,770 x g.
The Avanti JXN-26 centrifuge design features a brushless switchedreluctance drive motor,* an automatic rotor identification system, FRS
(friction reduction system), vacuum control circuitry, temperature
control system, programmable acceleration and deceleration profiles,
and a touch screen interface which shows both the information display
and the control input for the instrument. The JXN-26 includes many
options for configuring your system such as: managing your network,
users, rotors and reports.
1
Ergonomic design
• Low work surface height for easier
installation and unloading of rotors
5
• On-screen text sensitive “help” screen
• Foot pedal for hands-free door operation
2
• Up to 1000 user-defined programs
• Door swings up and out of the way for easy
access to the rotor chamber
• Set up and store numerous protocols
• Large digital readouts make operations
simpler
• Network multiple instruments
• Password protection for up to 50 unique user profiles
• Data logging and real-time run graphing
Gain flexibility with MobileFuge
• Set and monitor run parameters and email
diagnostic results
• Control and monitor the centrifuge from
your Apple® iOS and Android™ device
with MobileFuge
• Get alerts and diagnostics remotely
3
Advanced, easy-to-use data management
• Perfect for shared labs or GMP environments
Intuitive interface
• User-friendly, large LCD screen
• View set and actual run
conditions simultaneously
6
Versatile across rotors and applications
• Compatible with an extensive library of rotors
• Fixed-angle and swinging bucket options available
• Fully compatible with lightweight J-Lite rotors
• Dynamic Rotor Inertia Check (DRIC) and rotor recognition
provide redundant safety checks to assure no rotor can be
run above its rated speed
• Temperature control is optimized ensuring sample quality
and avoiding unwanted artifacts
• Select rotor by name, not
by arcane number code
• 11 accel/12 decel rates
• Delay start
• Run times to 99:59, Hold, and Ω 2t
integrator for applications that require
exquisite reproducibility
4
Safe and sustainable
• Friction Reduction System (FRS) allows
quicker, quieter, and cooler runs
• Uses less energy than traditional centrifuges
• BioSafe† options
• Dynamic rotor inertia check
• High-torque, Switched Reluctance (SR)
drive cycle shortens cycle times
* SR drives are manufactured by Beckman Coulter, Inc. with technology
licensed from Switched Reluctance Drives Limited.
† BioSafe is a term intended to describe the enhanced biocontainment
features of our products.
1-2
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
High Performance Centrifugation
Specifications
Avanti JXN-26
Maximum Speed/g-Force
26,000 rpm / 81,770 x g
Maximum Capacity
6 Liters
Speed Control
1
Low Speed ± 10 rpm of set speed
High Speed ± 0.1% rpm of set speed
Set Temperature
–10°C to 40°C in 1° increments
a
Temperature Control
± 2°C of chamber temperature after equilibration
Ambient Operating Range
16°C to 38°C
b
Accel / Decel Profiles
11 / 12
5,120 BTU/hr (1.5 kW)c
Heat Output
Drive Type / Cooling
SR* drive / Air-cooled
Refrigeration
Refrigerant 404A (HVC)
Sound Level
57 dBAd
Dimensions (with door closed)
71 W x 86 D x 86 H cm
(28 W x 34 D x 34 H in)
Weight
290 kg (640 lbs)
HEPA Filter
Pharmaceutical-grade sterilizing filter system
a: Proprietary algorithm applied to provide an estimated sample temperature.
b: To reach temperatures above ambient, the centrifuge is dependent on the frictional heat generated inside the chamber during
operation. At low run speeds or low ambient temperatures, the centrifuge may not be able to achieve some higher temperatures.
c: JA-20 rotor running at 20,000 rpm at 4°C.
d: JA-30.50 Ti rotor with chamber precooled and running at 26,000 rpm.
* SR drives are manufactured by Beckman Coulter, Inc. with technology licensed from Switched Reluctance Drives Limited.
Part Numbers**
230 V, 50 Hz, 24A
200–240 V,
50/60 Hz, 24A
220 V, 3-Phase,
50 Hz, 12A
Avanti JXN-26 IVD
B38623
B38619
B38618
Avanti JXN-26 Non-IVD
B37912
B34183
B34182
Model
Any unit can be made BioSafe† with addition
of HEPA filter kit:
B37916
HEPA filter kit for Avanti JXN-26 (external mount)
**Availability varies by geography. Contact your local sales representative to determine the correct
part numbers for ordering.
† BioSafe is a term intended to describe the enhanced biocontainment features of our products.
Beckman Coulter High Performance Centrifuges
1-3
www.beckmancoultercentrifugation.com
High Performance Centrifugation
1
Avanti J-26S XP Series High Performance Centrifuge
Bringing benchmark performance higher.
Already a leader in high performance, the Avanti J-26S has been
redesigned to provide even more versatility and safety. Above and
beyond its existing complete BioSafety § systems, the Avanti J-26S
has a more impact-resistant lid, full containment barrier, and
automatic rotor identification.
But improvements aren’t the only features that set the Avanti J-26S
apart. It’s built upon standard-setting performance, including high
speed, low heat output, imbalance tolerance and low noise. Its vast
range of separations only continues to grow. And its ergonomic
design—with lower instrument height and hands-free door
operation—remains unchanged.
1
2
3
3
5
Complete BioSafety§ systems
BioCertified rotors, instruments and labware
contain microbiological aerosols, while sterile
filters prevent infectious materials from being
expelled into the lab environment. Together,
they enhance peace of mind for users
and lab managers.
¥
Dual system rotor
recognition
Automatically detects which
rotor is installed and
prevents the rotor from
running above its maximumrated speed—without
shutting down the run.
All to ensure the safety
of your lab.
1-4
4
High performance
Application versatility: can process six liters
of fermentor output in less than 10 minutes,
deliver a subcellular fraction at 82,000 x g,
run 24 microplates at a time, and separate live
cell populations with elutriation.
Friction Reduction System
Enables quicker, quieter, more energy-efficient runs
with better temperature control, which improves system
reliability. High-torque, switched reluctance (SR) drive
technology shortens cycle times.
Lightweight J-Lite rotors
Consistently designed to be the most efficient rotors—for
reduced run times and high g-force.
2
4
6
5
7
6
1
7
Ergonomic design
Lower work surface for
easier rotor handling.
Foot pedal opens the door
and improved door design
swings up and out of the
way.
Sustainability is
built in
Each J-26S centrifuge will
contain approximately 7.5
pounds of recycled PET
bottles.
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
High Performance Centrifugation
Specifications
Avanti J-26S XP
Maximum speed/g-Force
26,000 rpm / 81,800 x g
Maximum Capacity
6 Liters
Speed Control
1
Low Speed ± 10 rpm of set speed
High Speed ± 0.1% rpm of set speed
Set Temperature
–10°C to 40°C in 1° increments
Temperature Control
± 2°C of set temperature
Ambient Operating Range
16°C to 38°C
Accel / Decel Profiles
2/3
Heat Output
6900 BTU/hr (2.0 kW)
Drive Type / Coolinge
SR* drive / Air-cooled
Refrigeration
Non-CFC, non-ozone
depleting refrigerant
Sound Level
57 dBA (0.91 m / 3 ft from
instrument at maximum speed)
Sterile Filter
Available
Dimensions (with door closed)
71 W x 86 D x 86 H cm
(28 W x 34 D x 34 H in)
Weight
290 kg (640 lbs)
User-Defined Programs
None
Time Settings
1 to 180 minutes, Hold
Delayed Start
No
Key Lock-Out
No
* SR drives are manufactured by Beckman Coulter, Inc. with technology licensed from Switched Reluctance Drives Limited.
Part Numbers
200/208/240 V
50/60 Hz
230 V
50 Hz
220/380 V
50 Hz, 3-Phase
Avanti J-26S XP
B14535
B14536
B14537
Avanti J-26S XP
with Elutriation
B14541
B14542
B14543
200/208/240 V
50/60 Hz
230 V
50 Hz
220/380 V
50 Hz, 3-Phase
B22984
B22985
B22987
Model
BioSafe
Package
Avanti J-26S XP
Beckman Coulter High Performance Centrifuges
1-5
www.beckmancoultercentrifugation.com
High Performance Centrifugation
1
Avanti J-E High Performance Centrifuge
Compact High Performance Centrifuge that fits directly in your lab
Whether your application is related to cellular, genetic, or
protein analysis, the Avanti J-E with speeds up to 21 000 rpm
(53 300 x g) coupled with powerful SR* drive technology provides you with fast separations.
A comprehensive line of labware, with up to twenty-four
96-well microtiter plate processing, 4 x 1L bottles, and
HarvestLine System Liners extend the versatility of this
centrifuge. For clinical research applications, conical tubes
and bottles for swinging bucket rotors spin volumes of 15, 50,
250, and 500 mL for effective blood component isolation with
a variety of gradient kits.
The J-E is energy efficient for low heat output plus the
quiet operation ensures a comfortable, productive work
environment.
Specifications
Maximum Speed
21 000 rpm
Maximum g-force
53 300 × g
Speed Control
Actual rotor speed, ± 50 rpm of
set speed
Drive Type
Switched Reluctance Drive*
Drive Cooling
Air-cooled
Centrifuge Warranty
1 year
Drive Warranty
2 years
Refrigeration System
Non-CFC, non-ozone-depleting
refrigerant
Max. Capacity Volume
4 000 mL
Ambient Temperature Range
15° to 35°C
Set Temperature Range
-10° to 40°C in 1° increments
Temperature Control
± 2°C of set
Time Setting
1 min. to 99:59 hours, hold
Acceleration/Deceleration Rates
2 accel/3 decel
Heat Output
2.0 kW (6 900 BTU/hr)
Sound Level
< 64 dBa (3 ft. from instrument
at maximum speed)
Dimensions
63.5 cm x 80 cm x 91.4 cm
(25 in. W x 31.5 in. D x 36 in. H)
Weight
267.4 kg (589 lb)
Avanti J-E High Performance Centrifuge
Part Numbers
Avanti J-E
Avanti J-E
208/240 V
50/60 Hz
230 V
50 Hz
200 V
50/60 Hz
369001
369003
369005
200/208/240 V
50/60 Hz
230 V
50/60 Hz
220/380 V
50/60 Hz
969352
A20698
A20699
* Manufactured under license from Switched Reluctance Drives Limited, Harrogate, UK.
1-6
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
High Performance Centrifugation
1
Avanti J-30I High Performance Centrifuge
The Maximum g-force for High Performance
The Avanti J-30I High Performance Centrifuge System gives
you the fastest separations possible—in the shortest amount
of time. Swinging bucket and fixed-angle rotors provide
maximum separation forces in excess of 100 000 x g at speeds
up to 30 000 rpm. Your productivity is ensured by unmatched
accel/decel rates and an easy run set-up. Your sample spends
more time at full force and less time in the centrifuge.
Specifications
Maximum Speed
30 000 rpm
Maximum g-force
110 500 × g
Speed Control
± 10 rpm of set speed or 0.15%,
whichever is greater
Imbalance Tolerance
± 2.5% opposing sample imbalance
Drive Type
Microprocessor-controlled Switched
Reluctance Drive*
Centrifuge Warranty
1 year
Drive Warranty
3 years
Drive Cooling
Air-cooled
Refrigeration System
Non-CFC, non-ozone depleting
refrigerant
Max. Capacity Volume
4 000 mL
Single Tube Sample Volume Range
1.5 – 1 000 mL
Set Temperature Range
-20° to 40°C in 1° increments
Temperature Control
± 2°C of set
User Defined Programs
Up to 30 two-step programs
Time Setting
1 min. to 99:59 hours, hold or ω2t
Acceleration/Deceleration Rates
12 accel/13 decel
Friction Reduction System
Yes
Zonal Capability
Yes
Dynamic Rotor Inertia Check
Automatic
Heat Output
2.0 kW (6900 BTU/hr)
Sound Level
< 57 dBa (3 ft. from instrument
at 30 000 rpm)
Dimensions
71 cm x 86 cm x 86 cm
(28 in. W × 34 in. D × 34 in. H)
Weight
310 kg (680 lb)
Avanti J-30I High Performance Centrifuge
Part Numbers
Avanti J-30I
Avanti J-30I
with Elutriation
Avanti J30I
200/208/240 V
60 Hz
230 V
50 Hz
220/380 V
50 Hz, 3-Phase
363118
363120
363121
200/208/240 V
50/60 Hz
230 V
50 Hz
220/380 V
50 Hz, 3-Phase
363122
363124
363125
200/208/240 V
50/60 Hz
230 V
50 Hz
220/380 V
50 Hz, 3-Phase
A20691
A20692
A20693
* Manufactured under license from Switched Reluctance Drives Limited, Harrogate, UK.
Beckman Coulter High Performance Centrifuges
1-7
www.beckmancoultercentrifugation.com
High Capacity Centrifugation
1
J6-MI High Capacity Centrifuge
It Spins Volumes
With six-liter capacity and an innovative range of rotors,
carriers, and adapters, this centrifuge can handle multiple
high-capacity applications. Ideal for bioprocessing, clinical
and blood sample preparation, the J6-MI is the standard for
quiet and convenient operation.
Its self-balancing rotor system corrects for imbalances
during a run, without shutdown. Its microprocessor-controlled
induction motor provides high torque, fast acceleration, and
long motor life. And the J6-MI generates speeds and forces
up to 6 000 rpm and 6 840 x g.
Specifications
Maximum Speed
6 000 rpm
Maximum g-force
6 840 × g
Speed Control
± 20 rpm of set speed
Drive Type
High-torque, variable-frequency
induction motor
Drive Cooling
Indirect drive system
Refrigeration System
Non-CFC, non-ozone-depleting
refrigerant
Max. Capacity Volume
6 Liters
Ambient Temperature Range
15° to 35°C
Set Temperature Range
–20°C to 40°C in 1° increments
Temperature Control
± 2°C of set temperature after
equilibration
Ambient Operating Range
16°C to 38°C
Acceleration/Deceleration Rates
9 accel/9 decel
Heat Output
< 7000 BTU/hr (1.9 kW)
Sound Level
< 68 dBa (0.91 m/3 ft. from instrument
at maximum speed)
Dimensions
71 cm x 84 cm x 91 cm
(25 in. W x 33 in. D x 36 in. H)
Weight
252 kg (5555 lb)
J6-MI High Capacity Centrifuge
Part Numbers
J6-MI High Capacity Centrifuge with
Microprocessor Control and
Brushless Induction Drive
208 V
60 Hz
240 V
50 Hz
220 V
50 Hz
360291
360292
360293
Note: Only values with tolerances or limits are guaranteed data. Values without tolerances are
informative data, without guarantee.
1-8
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
High Capacity Centrifugation
1
Avanti J-HC Centrifuge
The capacity to impress.
The Avanti J-HC High Capacity Centrifuge provides
you with a complete solution for batch bioprocessing.
A maximum of 9 liters can be spun at speeds of
5 000 rpm and 7 480 × g for greater sample throughput.
This allows processing of up to 36 liters per hour when
harvesting bacteria. Enhanced operator safety is provided with disposable HarvestLine System Liners.
Specifications
Avanti J-HC High Capacity Bioprocessing Centrifuge System
Maximum Speed
10 000 rpm
Maximum g-force
17,100 × g (JA-10 rotor @ 10 000 rpm)
Speed Control
± 25 rpm of set speed
Max. Capacity
9 000 mL
Single Bucket Volume
2 250 mL
Set Temperature Range
-10° to 40°C
Temperature Control
± 2°C
User Defined Programs
Up to 30 two-step programs
Friction Reduction
Automatic
Maximum Heat Dissipation to Room
2.0 kW (6 900 BTU/hr)
Noise Level
60 dBa (3 ft. from instrument
at 10 000 rpm)
Weight
300 kg (660 lb)
Dimensions
71 cm x 86 cm x 86 cm
(28 in. W x 34 in. D x 34 in. H)
Beckman Coulter High Performance Centrifuges
Part Numbers
Avanti J-HC
200/208/240 V
50/60 Hz
230 V
50 Hz
380 V
50 Hz, 3-Phase
367501
367502
367503
1-9
www.beckmancoultercentrifugation.com
High Performance/High Capacity Centrifugation
1
Rotor Compatibility Chart
Avanti J-30I
Max. RPM/
g-Force
Avanti J-26S
Max. RPM/
g-Force
Avanti J-E
Max. RPM/
g-Force
J6-MI
Avanti J-HC
Angle
Containment
JA-30.50 Ti
30 000
108 860
26 000
81 800
N/A
N/A
N/A
34°
Single- or dual-locking lid/
fluid annulus
JA-25.50†
25 000
75 600
25 000
75 600
21 000
53 300
N/A
N/A
34°
Single- or dual-locking lid/
fluid annulus
JA-25.15†
25 000
74 200
25 000
74 200
N/A
N/A
N/A
25°
Single- or dual-locking lid/
fluid annulus
JA-21
21 000
50 400
21 000
50 400
21 000
50 400
N/A
N/A
40°
JA-20.1†
20 000
51 500
20 000
51 500
20 000
51 500
N/A
N/A
23°
JA-20†
20 000
48 400
20 000
48 400
20 000
8 400
N/A
N/A
34°
JA-18.1
18 000
42 100
18 000
42 100
N/A
N/A
N/A
45°
N/A
JA-18†
18 000
47 900
18 000
47 900
16 000
37 800
N/A
N/A
23°
N/A
JA-17†
17 000
39 800
17 000
39 800
17 000
39 800
N/A
N/A
25°
JLA-16.250†
16 000
38 400
16 000
38 400
16 000
38 400
N/A
N/A
25°
Single- or dual-locking lid/
fluid annulus
JA-14.50†
14 000
35 000
14 000
35 000
14 000
35 000
N/A
N/A
35°
Single- or dual-locking lid/
fluid annulus
JA-14†
14 000
30 100
14 000
30 100
14 000
30 100
N/A
N/A
25°
JA-12†
12 000
23 200
12 000
23 200
12 000
23 200
N/A
N/A
35°
Single- or dual-locking lid/
fluid annulus
JLA-10.500†
10 000
18 500
10 000
18 500
10 000
18 500
N/A
N/A
20°
Sealing canister covers
JA-10
10 000
17 700
10 000
17 700
10 000
17 700
6 000
6 370
10 000
17 700
25°
JLA-9.1000
9 000
16 800
9 000
16 800
6 300
8 230
N/A
N/A
20°
Integrated bottle seal
JLA-8.1000†
N/A
8 000
15 970
N/A
N/A
20°
Integrated bottle seal
Avanti J-E
J6-MI
8 000
15 970
Fixed-Angle Rotors
Avanti J-30I
Avanti J-26S
Avanti J-HC
Angle
Containment
†This rotor was tested to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when used and maintained as instructed. Validation
of the microbiological containment was done at an independent, third-party facility (CAMR, Porton Down, UK or USAMARIID, Ft. Detrick, MD, USA). Improper use or maintenance may affect seal integrity
and, thus, containment.
1-10
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
High Performance /High Capacity Centrifugation
1
Rotor Compatibility Chart (continued)
Max. RPM/
g-Force
Swinging Bucket Rotors
JS-24.38
Max. RPM/
g-Force
JS-5.3
24 000
103 900
24 000
110 500
13 000
26 500
7 500
10 400
5 900
6 570
N/A
JS-4.3
N/A
JS-4.0
N/A
JS-5.2
N/A
N/A
N/A
5 200
6 840
N/A
Horz.
JS-5.0
N/A
N/A
N/A
N/A
5 000
7 480
Horz.
JS-4.2
N/A
N/A
N/A
4 200
5 020
4 200
5 020
Horz.
Aeroseal Cover
JS-4.2A
N/A
N/A
N/A
4 200
5 020
N/A
Horz.
Aeroseal Cover
JS-4.2SM
N/A
N/A
N/A
4 200
4 900
N/A
Horz.
JS-4.2SMA
N/A
N/A
N/A
4 200
4 900
N/A
Horz.
JS-4.0
N/A
N/A
N/A
4 000
4 050
N/A
Horz.
Aeroseal Cover
JS-3.0
N/A
N/A
N/A
3 000
2 560
N/A
Horz.
Aeroseal Cover
JS-24.15
JS-13.1
JS-7.5
JS-5.9
Avanti J-30I
Max. RPM/
g-Force
10 000
18 000
10 000
19 200
13 000
26 500
7 500
10 400
N/A
Max. RPM/
g-Force
5 300
6 130
4 300
4 220
4 000
4 050
Avanti J-26S
Max. RPM/
g-Force
Elutriation, Continuous Flow, and Zonal Rotors
JE-5.0
JCF-Z
Std. Core
JCF-Z
Large Core
JCF-Z
Small Core
JCF-Z
Reograd Core
JCF-Z
Zonal Core
N/A
20 000
39 900
20 000
39 900
20 000
36 300
20 000
39 900
20 000
39 900
5 000
4 700
20 000
39 900
20 000
39 900
20 000
36 300
20 000
39 900
20 000
39 900
Beckman Coulter High Performance Centrifuges
N/A
N/A
N/A
Horz.
Cap, O-ring
N/A
N/A
N/A
Horz.
Cap, O-ring
N/A
N/A
N/A
Horz.
N/A
N/A
N/A
Horz.
N/A
N/A
N/A
N/A
Horz.
N/A
5 300
6 130
N/A
N/A
N/A
Horz.
N/A
N/A
N/A
Horz.
Bucket Covers
N/A
N/A
N/A
Horz.
Aeroseal Cover
Aeroseal Cover
Avanti J-E
Max. RPM/
g-Force
N/A
N/A
N/A
N/A
N/A
N/A
1-11
www.beckmancoultercentrifugation.com
High Performance/High Capacity Centrifugation
1
High Performance and High Capacity Rotor Summary
Rotor
Type
Part
Number
Maximum
Speed
(rpm)
Maximum
Force at
rmin (g)
Maximum
Force at
rmax (g)
k Factor
Number Tubes/Bottles
and Size (diameter
x length) mm/in.
Rotor
Capacity
(mL)
Approx.
Comments
Accel. Time1
(min:sec)
30 000
40 320
108 860
280
8 x 50 mL
29 x 104 mm
(1.125 x 4 in.)
400 mL
5:15a
Harvesting bacteria, processing tissue culture, subcellular
particulates, routine pelleting
such as precipitates and
phase separation.
25 000
26 950
75 600
418
8 x 50 mL
29 x 104 mm
(1.125 x 4 in.)
400 mL
3:30b
High-force, efficient
pelleting of cell particles
from tissue homogenates.
Short column virus
purification.
25 000
36 400/
22 400
74 200/
60 200
265/
380
24 x 15 mL
18 x 100 mm
(0.75 x 4 in.)
360 mL
3:15b
High-force, efficient pelleting
of subcellular particles, bacteria, algae, and chloroplasts.
Short column banding of virus
and subcellular particles.
Fixed-Angle Rotors
JA-30.50 Ti
363420
Biosafety
363421
Single Lock Lid
JA-25.50
363058
Biosafety Lid
363055
Single Lock Lid
JA-25.15
363050
Biosafety Lid
363054
Single Lock Lid
JA-21
334845
21 000
22 200
50 400
470
18 x 10 mL
16 x 80 mm
(0.625 x 3 in.)
180 mL
1:30b
High-force, fast, efficient
separation of many samples in
small volume. Viruses, bacteriophage, mitochondria, nuclei.
JA-20.1
342095
20 000
28 700/
21 100
51 500/
43 900
371/
465
32 x 15 mL
18 x 99 mm
(0.625 x 4 in.)
480 mL
1:30b
High-force, large volume. Has
2 concentric rows of 15-mL
tubes. Efficient separation of
particles with 100 S or larger
sedimentation coefficients.
JA-20
334831
20 000
14 300
48 400
769
8 x 50 mL
29 x 104 mm
(1.125 x 4 in.)
400 mL
1:30b
Harvesting bacteria and cell
membranes, processing tissue
homogenates, separating
cell particulates.
JA-18.1
347824
18 000c
34 500c
42 100c
156c
24 x 1.8 mL
11 x 38 mm
(0.4 x 1.5 in.)
43.2 mL
1:20b
High-force sedimentation in
microcentrifuge-sized tubes
under refrigerated conditions.
Tube oriented at either a 25°
or 45° angle.
JA-18
369679
18 000
23 200
47 900
566
10 x 100 mL
38 x 102 mm
(1.5 x 4 in.)
1 liter
6:30b
High-force, large volume.
Pelleting bacteria, cell
membranes, and subcellular
organelles.
JA-17
369691
17 000
18 180
39 800
1 060
14 x 50 mL
29 x 104 mm
(1.125 x 4 in.)
700 mL
2:30b
Harvesting bacteria and cell
membranes, processing tissue
homogenates, separating
cell particulates.
JLA-16.250
363930
Biosafety Lid
16 000
13 200
38 400
1090
6 x 250 mL
62 x 120 mm
(2.5 x 5.5 in.)
1.5 liters
3:30b
Harvesting bacteria and cell
membranes, processing tissue
homogenates, separating
cell particulates.
14 000
19 000
35 000
787
16 x 50 mL
30 x 115 mm
800 mL
2:00
Pelleting and density gradient
separations, proteins, nucleic
acids, cells, viruses and blood.
363934 with
Single Lock Lid
JA-14.50
B19955
Biosafety Lid
B19956
Single Lock Lid
JA-14
339247
14 000
7 680
30 100
1 764
6 x 250 mL
62 x 120 mm
(2.5 x 4.8 in.)
1.5 liters
4:00b
General-purpose, largevolume, and multi-tube
processing.
JA-12
360992
Biosafety Lid
12 000
11 500
23 200
1 244
12 x 50 mL
30 x 103 mm
conical
(1.17 x 4 in.)
600 mL
3:30b
General pelleting of cells,
bacteria, and food products.
Separating of proteins, viruses,
and subcellular fractions. Phase
separations and binding studies.
360993 with
Single-locking Lid
1
Accel times are approximate, and subject to change.
b Typical accel time in Avanti J-25 Series.
1-12
a
c
Typical accel time in Avanti J-30I.
At a 45° angle.
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
High Performance/High Capacity Centrifugation
1
High Performance and High Capacity Rotor Summary (continued)
Rotor
Type
Part
Number
Maximum
Speed
(rpm)
Maximum
Force at
rmin (g)
Maximum
Force at
rmax (g)
k Factor
Number Tubes/Bottles
and Size (diameter
x length)
Rotor
Capacity
(mL)
Approx.
Comments
Accel. Time1
(min:sec)
Fixed-Angle Rotors (continued)
JLA-10.500
369681 with
Canisters
10 000
6 050
18 600
2 850
6 x 500 mL
69 x 160 mm
(2.75 x 6.5 in.)
3 liters
2:30a
Lightweight, high-volume, fixedangle rotor for initial processing
of tissue homogenate and other
large particles.
JA-10
369687
10 000
4 260
17 700
3 610
3 liters
4:30a
JLA-9.1000
366754
9 000
7 440
16 800
2 544
6
69
(2.75
4
95
(3.8
4 liters
2:30b
JLA-8.1000
363688
8 000
8 530
15 970
2 482
6 x 1000 mL
95 x 191 mm
(3.8 x 7.65 in.)
6 liters
6:00b
High-volume, fixed-angle rotor for
initial processing of tissue homogenate and other large particles.
General purpose, large-volume
processing, pelleting of bacteria
cell organelles, viruses, and
precipitates.
General purpose, large-volume
processing, pelleting of bacteria
subcellular organelles, viruses
and precipitates.
231 mL
5:15c
x
x
x
x
x
x
500 mL
160 mm
6.5 in.)
1000 mL
191 mm
7.65 in.)
Swinging-Bucket Rotors
JS-24.38
360743
24 000
48 600
103 900
334
6 x 38.5 mL
25 x 89 mm
(1 x 3.5 in.)
JS-24.15
362396
24 000
50 900
110 500
376
6 x 15 mL
16 x 96 mm
(0.625 x 3.75 in.)
90 mL
5:15c
JS-13.1
346963
13 000
7 760
26 500
1 841
6 x 50 mL
29 x 105 mm
(1.125 x 4 in.)
300 mL
2:30a
JS-7.5
336380
7 500
3 210
10 400
5 287
4 x 250 mL
62 x 136 mm
(2.5 x 5.5 in.)
1L
1:00a
JS-5.9
369331
5 900
5 400
6 570
various
10 microplates
JS-5.3
(AllSpin)
368690
2 920
5 170
6 870
various
4 x 500 mL
conical
24 microplates
2L
2:00d
JS-5.2*
339087
5 200
2 600
6 840
9 051
4 Liters
4 Blood Bags
12 Microplates
148 RIA-Tubes
4L
2:30
JS-5.0
367820
5 000
3 020
7 480
4 x 2.25 L
9L
3:30
JS-4.3
362734
3 250
1 532
4 220
4 x 750 mL
96 x 130 mm
(3.8 x 5.2 in.)
3L
1:00b
1
Accel times are approximate and subject to change.
a Typical accel time in Avanti J-25 Series.
b Typical accel time in Avanti J-26 XP Series.
* Only for J6-MI.
Beckman Coulter High Performance Centrifuges
11 800
1:00a
Harvesting bacteria, processing
tissue homogenates, subcellular
particulates, routine pelleting
such as precipitates and phase
separations.
Harvesting bacteria, processing
tissue homogenates, subcellular
particulates, routine pelleting
such as precipitates and phase
separations.
Density gradient centrifugation
of cells. Sedimentation of nuclei
and protein or nuclei acid precipitates. Clarification of tissue
homogenates.
Initial processing of cells and
removal of cell debris from culture
media. Accepts round-bottom
bottles for easier handling of
pellets.
High-throughput processing
(DNA or RNA kits), serial dilution
of small volumes.
Sucrose/glycerol gradients,
centrifugal filtration, binding
studies, clearing debris/large
particles, pelleting, plasma
protein precipitates.
Rapid sedimentation of protein
precipitates, large particles, cells,
and cell debris. It can be used for
binding studies and separating
serum from whole blood.
Separating bacterial yeast, and
tissue homogenates; harvesting
cultures.
Rapid sedimentation of
protein precipitates, large
particles, cells, binding
studies, and separating
serum from whole blood.
c Typical accel time in Avanti J-30I (JS rotors using mandatory slow accel setting).
d Typical accel time in Avanti J-E.
1-13
www.beckmancoultercentrifugation.com
High Performance/High Capacity Centrifugation
1
High Performance and High Capacity Rotor Summary (continued)
Rotor
Type
Part
Number
Maximum
Speed
(rpm)
Maximum
Force at
rmin (g)
Maximum
Force at
rmax (g)
k Factor
Number Tubes/Bottles
and Size (diameter
x length)
Rotor
Capacity
(mL)
Approx.
Accel
Time
Comments
Rapid sedimentation of protein
precipitates, large particles, cells,
and cell debris. It can be used for
binding studies and separating
serum from whole blood.
Rapid sedimentation of protein
precipitates, large particles, cells,
and cell debris. It can be used for
binding studies and separating
serum from whole blood.
Separation of serum from whole
blood, blood component separation.
Separation of serum from whole
blood, blood component separation.
Swinging-Bucket Rotors (continued)
JS-4.2
339080
4 200
2 250
5 020
11 504
6 Liters, 6 Blood Bags,
18 Microplates,
336 RIA-Tubes
6L
2:30
JS-4.2A**
366695
4 200
2 250
5 020
11 504
6 Liters, 6 Blood Bags,
18 Microplates,
336 RIA-Tubes
6L
2:30
JS-4.2/SM**
348394
4 200
2 290
4 900
JS-4.2/SMA** 366670
4 200
2 290
4 900
JS-4.0
339086
4 000
1 540
4 050
JS-3.0*
339086
3 000
1 150
2 560
6 Blood Bags
18 Microplates
6 Blood Bags
18 Microplates
15 296
2:30
2:30
4 x 000
97 x 167
(3.82 x 6.57 in.)
4L
6 x 1000
97 x 167
6L
2:00b
Rapid sedimentation of protein
precipitates, large particles, cells
and cell debris, as well as binding
studies and separating serum
from whole blood.
Continuous-Flow Rotor
JCF-Z
335140
(standard
core)
20 000
32 000
39 900
100
N/A
660 mL
Purification of viruses and/or
concentration of large volumes
of solution. Pelleting or sedimenting on a sucrose cushion.
Maximum pellet size: 400 mL.
357544
(small pellet
core)
20 000
23 300
36 300
281
N/A
240 mL
Pelleting liquids that contain
a low ratio of solids–such as
bacterial cultures, or water containing clay particles or algae.
Maximum pellet size: 200 mL.
336283
(large pellet
core)
20 000
22 850
39 900
293
N/A
1 250 mL
Pelleting solutions that have
high solid-to-water ratios as
high as 1:2 slurries. Maximum
pellet size: 800 mL.
20 000
9 000
39 900
710
69 mm
1 900 mL
Fast start up. Dynamic loading
and unloading. Isopycnic banding, linear, and discontinuous
gradients. Subcellular fractions from tissue homogenates, algae, and chloroplasts.
20 000
11 650
39 900
779
63 mm
1 750 mL
No rotating seal. Static loading
and unloading. Gradient reorients in rotor. Especially useful
for fragile material such as
DNA strands.
5 000
2 410
4 700
6 785
———
40 mL
The counterflow centrifugation
elutriation system is a gentle, yet
powerful technique for harvesting
large populations of living cells,
resulting in high viability rates.
Zonal Rotor
JCF-Z
354006
Reorienting Gradient Rotor
JCF-Z
354005
Elutriation
JE-5.0
356900
*JS-3.0 rotor is for use in Beckman Coulter J6 Series centrifuges only per IFU J6-TB-0075C.
**Only for J6.
1-14
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
Protein
Pelleting ammonium
sulfate precipitation
Sucrose/glycerol gradient
Centrifugal filtration 1-50 mL
Subcellular
Centrifugal filtration <1.0 mL
Fractions
Chromatin/
Sucrose gradient isolation
Nucleosomes
Microsomes Pelleting
Microsomal membrane fraction
Mitochondria Pelleting
Sucrose gradient isolation
Nucleic Pelleting
Cell
Pelleting
Membranes
Sucrose gradient isolation
Binding studies
Ribosomes/
Pelleting
Polysomes
Sucrose gradient isolation
Cytosol Clarification
Lysate/Tissue
Clearing debris and large particles
Homogenates
Clearing Media Clearing debris and large particles
Nucleic Acids Pelleting alcohol precipitation
Phenol/chloroform extraction
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■ ■
■
■ ■
■ ■
■ ■
■ ■
■ ■
■
■ ■
■ ■
■
■ ■
■ ■
■
■
■ ■
■
■ ■ ■ ■ ■ ■ ■ ■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■ ■
■
■
■ ■ ■
■
■
■ ■ ■
■
■
■
■
■
■
■
■
■
■
■
■
■
■ ■ ■ ■
■ ■ ■ ■
■
■
■
Spin columns
Pelleting mammalian cells
Density gradient separation
Viruses Pelleting
PEG precipitates
Density gradient isolation
Blood Pelleting
Plasma protein precipitation
JS-4.2
JS-5.3
JS-13.1
JS-24.38
■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■
■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■ ■
■ ■
■ ■ ■ ■ ■
■
■ ■ ■ ■
■
■
■ ■
Minipreps in 96-well plates
Cells Pelleting bacteria
JLA-8.1000
JLA-9.1000
JA-10
JLA-10.500
JA-12
JA-14
JA-14.50
JLA-16.250
JA-17
JA-18.1
JA-18
JA-20
JA-20.1
JA-21
JA-25.15
Specific Application
JA-25.50
Bioseparation
JA-30.50 Ti
ROTOR SELECTION BY APPLICATION†
Blood sample preparation
Density gradients to
isolate blood cells
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■
■ ■ ■ ■
■ ■ ■ ■
■ ■ ■ ■
■
■ ■ ■
■
■ ■ ■ ■
■ ■ ■ ■
■
■ ■ ■ ■ ■
■ ■ ■ ■ ■
■ ■
■ ■
■ ■ ■ ■ ■
■ ■ ■ ■
■
■ ■ ■
■ ■
■ ■
■ ■
■
■
■ ■
■
■
■ ■ ■ ■
■ ■ ■ ■
■ ■ ■
■
■ ■ ■
■
■
■ ■ ■ ■
■ ■
■ ■ ■ ■
■ ■ ■
■
■
■
■
■
■
■
■
■
■
Maximum rotor speeds may differ between instrument models. For complete rotor specifications, please refer to our High Performance and
High Capacity centrifuge catalog or visit beckmancoulter.com.
◆
³BioSafe when used with Aeroseal covers. ⁴BioSafe when used with Aerosolve canisters. ⁵J6-M� must have door and strobe.
†Selected rotor has the capability (x g, volume, labware) to accommodate the application, but may not be the most optimal/efficient choice for
the specific application.
All labware sold separately.
Beckman Coulter High Performance Centrifuges
1-15
1
www.beckmancoultercentrifugation.com
JA-30.50 Ti
JA-30.50 Ti
8 x 50 mL
Fixed-Angle Rotor, Titanium
Major applications: Harvesting bacteria, processing tissue homogenates,
subcellular particulates, routine pelleting such as precipitates and phase
separations.
Axis of Rotation
34°
1
rmin
(40 mm)
rav (74 mm)
rmax (108 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
30 000
108 860
280
8 × 50 mL
29 × 104 mm
1.125 × 4 in
400 mL
For use in Avanti J Series centrifuges
(except Avanti J-E and J-HC) centrifuges.
No. 363420. JA-30.50 Fixed-Angle Ti Rotor with Single Lock Lid.
No. 363421. JA-30.50 Fixed-Angle Ti Rotor with Single Lock Lid.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
———
———
75 600
403
25 000
1
———
91 000
75 600
244
403
30 000
25 000
Bottles with Screw-On Caps
Polypropylene
50.0
357003
25
29 x 104
Polycarbonate
10.0
50.0
355672
357002
25
25
16 x 80 361703 (qty. of 2)
29 x 104
———
Polypropylene
50.0
357001
6
29 x 104
———
———
108 800
280
30 000*
Polycarbonate
50.0
357000
6
29 x 104
———
———
108 800
280
30 000
1.5
357448
500
11 x 38
361705 (qty. of 2)
3
73 400
126
30 000
50.0
363664
25
29 x 104
———
———
75 600
403
25 000
Bottles with Cap Assemblies
Tubes with Snap-On Caps
Polypropylene
Thickwall Polycarbonate
Polypropylene
1.5
356090
500
11 x 38
361705 (qty. of 2)
3
73 400
126
30 000
50.0
357005
25
29 x 104
———
———
75 600
403
25 000
Thickwall Polycarbonate
50.0
363647
25
29 x 104
———
———
108 800
280
30 000*
Thickwall Polypropylene
50.0
357007
25
29 x 104
———
———
108 800
280
30 000
———
———
———
———
———
12 x 75
13 x 100
16 x 80
18 x 80
29 x 104
356970 (qty. of 1)
356976 (qty. of 1)
342327 (qty. of 1)
870329 (qty. of 1)
870331 (qty. of 1)
1
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Thickwall Polypropylene
Open-Top Tubes
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
5.0
8.0
10.0
15.0
30.0
———
———
———
———
———
* At 2°C; at 25°C, maximum speed must be limited to 26 000 rpm for tube 357001 and
29 000 rpm for tube 363647.
Rotor Replacement Parts
870612
11757
363424
363430
363431
O-ring for Rotor Lid
Small O-ring for Rotor Lid
Lid Assembly, Biosafety (tie-down assembly included)
Lid Assembly, Single-locking
Tie-down for Single-locking Lid
Adapters
361703
1-16
361705
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JA-25.50
JA-25.50
8 x 50 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
Major applications: Harvesting bacteria, processing tissue homogenates,
subcellular particulates, routine pelleting such as precipitates and phase
separations.
rmin (38.5 mm)
rav (73.2 mm)
rmax (108 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
25 000
75 600
418
8 x 50 mL
29 x 104 mm
1.125 x 4 in
400 mL
34°
For use in Avanti J Series centrifuges.
No. 363058. JA-25.50 Fixed-Angle Rotor with Biosafety Lid.
No. 363055. JA-25.50 Fixed-Angle Rotor with Single Lock Lid.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
———
———
75 600
418
25 000
1
———
63 240
75 600
350
418
25 000
25 000
Bottles with Screw-On Caps
Polypropylene
50.0
357003
25
29 x 104
Polycarbonate
10.0
50.0
355672
357002
25
25
16 x 80 361703 (qty. of 2)
29 x 104
———
Polypropylene
50.0
361694
24
29 x 104
———
———
75 600
418
25 000
Polycarbonate
50.0
361693
24
29 x 104
———
———
75 600
418
25 000
———
———
75 600
418
25 000
3
———
50 960
75 600
182
418
25 000
25 000
Bottles with Cap Assemblies
Tubes with Snap-On Caps
Thickwall Polycarbonate
50.0
363664
25
29 x 104
Thickwall Polypropylene
1.5
50.0
356090
357005
500
25
9.5 x 38 361705 (qty. of 2)
29 x 104
———
Open-Top Tubes
Polypropylene
1.5
357448
500
9.5 x 38 361705 (qty. of 2)5
3
50 960
182
25 000
Thickwall Polycarbonate
50.0
363647
25
29 x 104
———
———
75 600
418
25 000
Thickwall Polypropylene
50.0
357007
25
29 x 104
———
———
75 600
418
25 000
———
———
———
———
———
12 x 75
13 x 100
16 x 80
18 x 80
29 x 104
356970 (qty. of 1)
356976 (qty. of 1)
342327 (qty. of 1)
870329 (qty. of 1)
870331 (qty. of 1)
1
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
*
5.0
8.0
10.0
15.0
30.0
———
———
———
———
———
Order cap separately (pkg. of 25): 356264 (natural), 357359 (red), 357360 (green), 357361
(yellow), or 357362 (blue).
Rotor Replacement Parts
870612
11757
363023
363053
O-ring for Rotor Lid
Small O-ring for Rotor Lid
Lid Assembly, Biosafety (Tie-down Assembly included)
Lid Assembly, Single Lock (Tie-down Assembly included)
Adapters
361703
361705
Beckman Coulter High Performance Centrifuges
1-17
1
www.beckmancoultercentrifugation.com
JA-25.15
JA-25.15
24 x 15 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
Major applications: Harvesting bacteria, processing tissue homogenates,
subcellular particulates, routine pelleting such as precipitates and phase
separations.
rmin (52 mm)
1
rav (79 mm)
rmax (106 mm)
25°
Axis of Rotation
rmin (32 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor
Capacity
Outer Row
25 000
74 200
265
360 mL
Inner Row
25 000
60 200
380
24 x 15 mL
18 x 100 mm
0.75 x 4 in
For use in Avanti J Series centrifuges
(except Avanti J-E) centrifuges.
rav (59 mm)
rmax (86 mm)
25°
No. 363050. JA-25.15 Fixed-Angle Rotor with Biosafety Lid.
No. 363054. JA-25.15 Fixed-Angle Rotor, Single Lock Lid.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
(Outer Row)
Bottles with Screw-On Caps
Polycarbonate
10.0
355672
25
16 x 80
342327
1
60 200
204
25 000
Thickwall Polypropylene
4.0
10.0
355644
355640
25
25
13 x 64
16 x 75
303313
342327
1
1
49 000
56 700
120
180
25 000
25 000
Thickwall Polycarbonate*
4.0
10.0
15.0
355645
355630
342080
25
25
100
13 x 64
16 x 75
18 x 98
303313
342327
———
1
1
———
49 000
56 700
74 200
120
180
265
25 000
25 000
25 000
Thickwall Polyethylene*
15.0
342081
100
18 x 98
———
———
74 200
265
25 000
Thickwall Polypropylene*
15.0
342082
100
18 x 98
———
———
74 200
265
25 000
———
12 x 75
342329 (qty. of 1)
1
———
———
———
Open-Top Tube
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
*
3.0
———
To order caps for 15-mL tubes 342080, 342081, and 342082, use part number 343656 for a package
of 50.
.
Rotor Replacement Parts
964911
11757
363024
363052
Large O-ring for Rotor Lid
Small O-ring for Rotor Lid
Lid Assembly, Biosafety (Tie-down Assembly included)
Rotor Lid Assembly, Single Lock (Tie-down Assembly
included)
Adapters
303313
1-18
342327
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JA-21
JA-21
18 x 10 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
rmin (45 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
21 000
50 400
470
18 x 10 mL
16 x 80 mm
0.625 x 3 in
180 mL
rav (73 mm)
1
rmax (102 mm)
40°
For use in Avanti J Series centrifuges.
No. 334845. JA-21 Fixed-Angle Rotor for 21 000 rpm operation.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polycarbonate
10.0
355672
25
16 x 80
———
———
50 400
470
21 000
Thickwall Polypropylene
10.0
355640
25
16 x 76
———
———
50 400
470
21 000
Thickwall Polycarbonate
10.0
355630
25
16 x 76
———
———
50 400
470
21 000
———
12 x 75
356972 (qty. of 1)
1
———
———
———
Open-Top Tubes
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
5.0
———
Rotor Replacement Parts
870612
870980
335107
343631
369349
Beckman Coulter High Performance Centrifuges
O-ring for Rotor Lid
O-ring for Rotor Hub
Rotor Lid
Lid Attachment Bolt Assembly
Rotor Lid Assembly (Tie-down Assembly included)
1-19
www.beckmancoultercentrifugation.com
JA-20.1
JA-20.1
32 x 15 mL
Fixed-Angle Rotor, Aluminum
Major applications: High-force, large-volume separation of particles with
100 S or larger sedimentation coefficients.
1
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor
Capacity
Outer Row
20 000
51 500
371
480 mL
Inner Row
20 000
43 900
465
32 x 15 mL
18 x 99 mm
0.625 x 4 in
For use in Avanti J Series centrifuges.
No. 342095. JA-20.1 Fixed-Angle Rotor for 20 000 rpm operation.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor*
Maximum
Speed
(Outer Row)
Bottles with Screw-On Caps
Polycarbonate
10.0
355672
25
16 x 80
342327
1
47 900
325
20 000
Thickwall Polypropylene
10.0
355640
25
16 x 76
342327
1
47 900
325
20 000
Thickwall Polycarbonate
10.0
15.0
355630
342080
25
100
16 x 76
18 x 98
342327
———
1
———
47 900
51 500
325
371
20 000
20 000
Thickwall Polyethylene
15.0
342081
100
18 x 98
———
———
51 500
371
20 000
Thickwall Polypropylene
15.0
342082
100
18 x 98
———
———
51 500
371
20 000
———
———
———
———
———
12 x 75
12 x 75
13 x 100
16 x 99
18 x 99
342329 (qty. of 1)
356971 (qty. of 1)
356977 (qty. of 1)
342348 (qty. of 1)
342643 (qty. of 1)
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Open-Top Tubes
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
3.0
5.0
8.0
15.0
15.0
———
———
———
———
———
1
1
Caps for 15-mL Tubes
Rotor Replacement Parts
343656
834301
811656
358368
341976
364915
392889
*
Caps (box of 50) for 342080, 342081, and 342082 Tubes
O-ring for Rotor Lid
O-ring for Rotor Body
Lid Attachment Bolt Assembly
Rotor Lid
Tie-down Kit
Hold-down Knob, for use with filtration tubes regularly
run without lid (in J2/J6 centrifuges only)
338896 Rotor Removal Tool
338689 Adapter Collar for 338896 Rotor Removal Tool
Measured using the outer row of tubes.
Adapters
342327
1-20
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JA-20
JA-20
8 x 50 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
Major applications: Harvesting bacteria and cell membranes, processing
tissue homogenates, separating cell particulates.
rmin (32 mm)
rav (70 mm)
rmax (108 mm)
34°
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
20 000
48 400
769
8 x 50 mL
29 x 104 mm
1.125 x 4 in
400 mL
For use in Avanti J Series centrifuges.
No. 334831. JA-20 Fixed-Angle Rotor for 20 000 rpm operation.
Tubes and Bottles
Tube Style/Material
Minimum
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polyethylene
50.0
357003
25
29 x 104
———
———
48 400
769
20 000
Polycarbonate
10.0
50.0
355672
357002
25
25
16 x 80
29 x 104
342327/870329
———
1
———
43 500
48 400
650
769
20 000
20 000
Polypropylene
50.0
357001
6
29 x 104
———
———
48 400
769
20 000
Polycarbonate
50.0
357000
6
29 x 104
———
———
48 400
769
20 000
1.5
357448
500
11 x 38
344497*
6
48 400
769
20 000
50.0
363647
25
29 x 104
———
———
48 400
769
20 000
Bottles with Cap Assemblies
Tubes with Snap-On Caps
Polypropylene
Natural
Thickwall Polycarbonate
Polyethylene
Thickwall Polypropylene
Orange
Yellow
Green
Blue
Natural
1.8
340196
500
11 x 39
344497*
6
48 400†
769
20 000
50.0
357005
25
29 x 104
———
———
48 400
769
20 000
1.5
1.5
1.5
1.5
1.5
356094
356093
356092
356091
356090
500
500
500
500
500
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
344497*
344497*
344497*
344497*
344497*
6
6
6
6
6
48 400
48 400
48 400
48 400
48 400
769
769
769
769
769
20 000
20 000
20 000
20 000
20 000
50.0
357007
25
29 x 104
———
———
48 400
769
20 000
———
———
———
———
12 x 75
13 x 100
18 x 80
29 x 104
356970 (qty. of 1)
356976 (qty. of 1)
870329 (qty. of 1)
870331 (qty. of 1)
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
Open-Top Tubes
Thickwall Polypropylene
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
5.0
8.0
15.0
30.0
———
———
———
———
Rotor Replacement Parts
Cap Assembly Replacement Parts
870612
811656
364914
368998
392889
356284
358627
O-ring for Rotor Lid
O-ring for Rotor Hub
Tie-down Kit
Rotor Lid
Hold-down Knob, for use with filtration tubes regularly
run without lid (in J2/J6 centrifuges only)
Rotor Removal Tool
Adapter Collar for 338896 Rotor Removal Tool
338896
338689
*
†
961582
Cap for 357000 and 357001 Bottle
Insert for 357000 and 357001 Cap Assembly;
order only as a replacement part
O-ring for 357000 and 357001 Bottle
Can be double-stacked. Force will vary depending on tube position.
Calculated at rmax, g-force will be less for tubes located in upper adapter when adapters
are stacked. Force will also vary depending on tube position in adapter.
Adapters
342327
344497
870329
Beckman Coulter High Performance Centrifuges
1-21
1
www.beckmancoultercentrifugation.com
JA-18.1
JA-18.1
24 x 1.8 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
1
Major applications: High-force sedimentation in microcentrifuge tubes
under refrigerated conditions.
rmin (95.0 mm)
rav (105 mm)
rmax (116 mm)
Max. RPM
at 45° Angle*
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
18 000
42 100
156
24 x 1.8 mL
11 x 38 mm
0.4 x 1.5 in.
43.2 mL
Max. RPM
at 25° Angle*
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
17 000
36 300
91
24 x 1.8 mL
11 x 38 mm
0.4 x 1.5 in.
43.2 mL
45°
Axis of Rotation
rmin (101 mm)
rav (106 mm)
rmax (112 mm)
For use in Avanti J Series centrifuges
(except Avanti J-E).
25°
No. 347824. JA-18.1 Fixed-Angle Rotor. Includes 24 reversible adapters.
Tubes and Bottles
Tube Style/Material
Minimum
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters*
Tubes per
Adapter†
g-Force
k Factor
Maximum
Speed*
Tubes with Snap-On Caps
Polypropylene
Natural
Polyethylene
Polypropylene
*
Orange
Yellow
Green
Blue
Natural
Natural‡
1.5
357448
500
11 x 38
347562
1
42 100
———
18 000
1.8
340196
500
11 x 39
347562
1
42 100
———
18 000
1.5
1.5
1.5
1.5
1.5
1.5
356094
356093
356092
356091
356090
343169
500
500
500
500
500
500
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
347562
347562
347562
347562
347562
347562
1
1
1
1
1
1
42 100
42 100
42 100
42 100
42 100
42 100
———
———
———
———
———
———
18 000
18 000
18 000
18 000
18 000
18 000
Adapters included with rotor. Adapters are reversible, so that tube angle can be set at either
45° or 25°, depending on application requirements.
Rotor Replacement Parts
11921
347821
364918
O-ring
Rotor Knob Assembly
Tie-down Kit
Adapters
347562
1-22
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JA-18
JA-18
10 x 100 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
Major applications: High-force, large-volume. Pelleting bacteria, cell
membranes, and subcellular organelles.
Max. RPM
rmin (64 mm)
rav (98 mm)
Max. g
18 000
47 900
(16 000 rpm* at
4°C and below)
rmax (132 mm)
23°
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
566
10 x 100 mL
38 x 102 mm
1.5 x 4 in
1L
For use in Avanti J Series centrifuges.
No. 369679. JA-18 Fixed-Angle Rotor for 18 000 rpm operation.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Quick-Seal Tubes
Polypropylene
100.0
345776
25
38 x 102
———
———
47 900
566
18 000
Ultra-Clear
100.0
345778
25
38 x 102
———
———
47 900
566
18 000
Polycarbonate
70.0
355620
6
38 x 102
———
———
47 900
566
18 000
Polypropylene
94.0
355624
6
38 x 102
———
———
47 900
566
18 000
Bottles with Cap Assemblies
Bottles with Screw-On Caps
Polypropylene
50.0
357003
25
29 x 104
347539
1
44 000
———
18 000
Polycarbonate
50.0
357002
25
29 x 104
347539
1
44 000
———
18 000
1.5
1.5
1.5
1.5
1.5
1.5
1.5
356094
356093
356092
356091
356090
357448
343169
500
500
500
500
500
500
500
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
344497/347539†**
344497/347539†**
344497/347539†**
344497/347539†**
344497/347539†**
344497/347539†**
344497/347539†**
6
6
6
6
6
6
6
35 000
35 000
35 000
35 000
35 000
35 000
35 000
———
———
———
———
———
———
———
18 000
18 000
18 000
18 000
18 000
18 000
18 000
1.8
340196
500
11 x 39
344497/347539†**
6
35 000
———
18 000
Thickwall Polycarbonate*
50.0
363647
25
29 x 104
347539
1
44 000
———
18 000
Thickwall Polypropylene*
50.0
357007
25
29 x 104
347539
1
44 000
———
18 000
———
———
———
———
———
———
12 x 75
13 x 100
17 x 120
18 x 80
29 x 104
30 x 115
3
3
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Tubes with Snap-On Caps
Polypropylene
Orange
Yellow
Green
Blue
Natural
Polypropylene
Natural‡
Polyethylene
Open-Top Tubes
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
Round Bottom
Conical
Round Bottom
Round Bottom
Conical
5.0
10.0
15.0
15.0
30.0
50.0
———
———
———
———
———
———
*
A cap is available for these tubes, but it cannot be used in the JA-18 rotor; for a cap
to use when storing samples, order cap 356262.
† Can be double-stacked. Force will vary depending on tube position.
‡ With separate cap.
.** These adapters fit inside one another to accommodate smaller bottles and tubes.
356969 (qty. of 1)
356975 (qty. of 1)
356962 (qty. of 1)
870329/347539 (qty. of 1)
870331/347539 (qty. of 1)
356963 (qty. of 1)
Rotor Replacement Parts
346947
346965
364845
364917
Tie-down Bolt Assembly
Rotor Removal Tool
Rotor Lid Assembly
Tie-down Kit, IRIS
Adapters
344497
347539
Beckman Coulter High Performance Centrifuges
1-23
1
www.beckmancoultercentrifugation.com
JA-17
JA-17
14 x 50 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
Major applications: High-force, large-volume. Pelleting bacteria, cell
membranes, and subcellular organelles.
rmin (56 mm)
1
rav (90 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
17 000
39 800
690
14 x 50 mL
29 x 104 mm
1.125 x 4 in
700 mL
rmax (123 mm)
For use in Avanti J Series centrifuges.
25°
No. 369691. JA-17 Fixed-Angle Rotor.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Cap Assemblies
Polypropylene
50.0
50.0
357001
361694
6
24
29 x 104
29 x 104
———
———
———
———
39 800
39 800
690
690
17 000
17 000
Polycarbonate
50.0
50.0
357000
361693
6
24
29 x 104
29 x 104
———
———
———
———
39 800
39 800
690
690
17 000
17 000
Polypropylene
50.0
357003
25
29 x 104
———
———
39 800
690
17 000
Polycarbonate
10.0
50.0
355672
357002
25
25
16 x 80
29 x 104
342327/870329
———
1
———
32 000
39 800
500
690
17 000
17 000
50.0
357005†
25
29 x 104
———
———
39 800*
690
17 000
Thickwall Polycarbonate
50.0
363647
25
29 x 104
———
———
39 800
690
17 000
Thickwall Polypropylene
50.0
357007
25
29 x 104
———
———
39 800
690
17 000
———
———
———
———
———
12 x 75
13 x 100
16 x 80
18 x 80
29 x 104
1
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Bottles with Screw-On Caps
Tubes with Snap-On Caps
Polypropylene
Open-Top Tubes
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
5.0
8.0
10.0
15.0
30.0
———
———
———
———
———
356970 (qty. of 1)
356976 (qty. of 1)
342327/870329 (qty. of 1)
870329 (qty. of 1)
870331 (qty. of 1)
Cap Assembly Replacement Parts
Rotor Replacement Parts
356284
358627
878951
811656
369760
364913
392889
961582
†
Cap for 357000 and 357001 Tubes
Insert for 357000 and 357001 Cap Assembly;
order only for use in tubes
O-ring for 357000 and 357001 Cap Assembly
A maximum of seven tubes can be used, placed in every other rotor hole (tubes placed in adjacent
holes will hit one another).
O-ring for Rotor Lid
O-ring for Rotor Hub
Lid Assembly
Tie-down Kit
Hold-down Knob
(used to run filtration tubes without lid)
Rotor Supplies
338896
338689
356970
356976
Rotor Removal Tool
Adapter Collar for 338896 Rotor Removal Tool
Acetal Adapter (qty. 1), fits 12 mm tubes
Acetal Adapter (qty. 1), fits 13 mm tubes
Adapters
342327
1-24
870329
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JLA-16.250
JLA-16.250
6 x 250 mL
Fixed-Angle Rotor, Aluminum
25°
Major applications: General purpose, large-volume and multitube
processing. Lightweight alternative to conventional 6 × 250 rotors;
empty rotor weight is 10.3 kg (22.7 lb).
rmin
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
16 000
38 400
1 090
6 × 250 mL
62 × 120 mm
2.5 × 5.5 in
1.5 L
46 mm
rav
90 mm
rmax
134 mm
For use in Avanti J Series centrifuges.
Axis of Rotation
No. 363930. J-LITE JLA-16.250 Rotor Assembly with Biosafety Lid.
No. 363934. Rotor Assembly with Single Lock Lid.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polypropylene
50.0
357003
25
29 x 104
356997
1
32 200
650
16 000
Polycarbonate
50.0
357002
25
29 x 104
356997
1
32 200
660
16 000
Polypropylene
50.0
357001
6
29 x 104
356997
1
32 200
650
16 000
Polycarbonate
50.0
357000
6
29 x 104
356997
1
32 400
660
16 000
Wide-mouth Polycarbonate
250.0
356013
6
62 x 120
——
——
38 500
1 090
16 000
Wide-mouth Polypropylene
250.0
356011
6
62 x 120
——
——
38 500
1 070
16 000
Polycarbonate
50.0
363664
25
29 x 104
356997
1
32 100
400
16 000
Polypropylene
50.0
357005
25
29 x 104
356997
1
32 100
400
16 000
4.0
566353
1000
14 x 55
342098
9
2 300
10 700
4 000
———
———
———
———
———
12 x 75
13 x 100
17 x 119
30 x 104
30 x 104
356968 (qty. of 1)
356974 (qty. of 1)
356964 (qty. of 1)
356966 (qty. of 1)
356997 (qty. of 1)
9
8
4
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Bottles with Cap Assemblies
Tubes with Snap-On Caps
BioVials
Polypropylene
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
Round Bottom
Conical
Conical
Round Bottom
5.0
7.0
15.0
50.0
50.0
———
———
———
———
———
Rotor Replacement Parts
363931
363935
893502
893503
Double-locking Lid Assembly
Single-locking Lid Assembly
Large O-ring for Lid Assembly (5.987 dia.)
Small O-ring for Lid Assembly (1.046 dia.)
Adapters
342098
356997
356964
Beckman Coulter High Performance Centrifuges
1-25
1
www.beckmancoultercentrifugation.com
JA-14.50
JA-14.50
16 x 50 mL
Fixed-Angle Rotor, Aluminum
Major applications: Pelleting and Density Gradient Separations. Proteins,
lysate/tissue homogenates, nucleic acids, cells, viruses and blood.
1
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
14 000
35 000
787
16 x 50 mL Conical 800 mL
30 x 115 mm
For use in Avanti J Series centrifuges.
Axis of Rotation
No. B19956. JA-14.50 Fixed-Angle Rotor with Single-Locking Lid for 14000 rpm operation. Tubes and bottles not included.
No. B19955. JA-14.50 Fixed-Angle Rotor for BioSafe Dual-Locking Lid for 14000 rpm operation. Tubes and bottles not included.
Tubes and Bottles
Tube Style/Material
Bottles with Screw-On Caps
Polycarbonate Round Bottom
Polypropylene Round Bottom
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Size (mm) /
volume (mL)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
40.0
40.0
357002
357003
25
25
29 x 104/50 B40155 (qty 2)
29 x 104/50 B40155 (qty 2)
1
1
34 520
34 520
563
563
14 000
14 000
Polycarbonate Round Bottom
Polypropylene Round Bottom
40.0
40.0
361693
361694
24
24
29 x 104/50 B40155 (qty 2)
29 x 104/50 B40155 (qty 2)
1
1
34 520
34 520
563
563
14 000
14 000
Polycarbonate Round Bottom
10.0
355672
25
16 x 80/10
361703 and
B40155 (qty 2)
1
30 900
445
14 000
36.5
36.5
363664
357005
25
25
29 x 104/50 B40155 (qty 2)
29 x 104/50 B40155 (qty 2)
1
1
34 520
34 520
563
563
14 000
14 000
Polycarbonate Round Bottom
35.0
363647
25
29 x 104/50 B40155 (qty 2)
1
34 520
563
14 000
Polypropylene Round Bottom
35.0
357007
25
29 x 104/50 B40155 (qty 2)
1
34 520
563
14 000
Polycarbonate Round Bottom
15.0
342080
100
18 x 100/15 B40157 (qty 2)
1
33 400
665
14 000
Polypropylene Round Bottom
15.0
342081
100
18 x 100/15 B40157 (qty 2)
1
33 400
665
14 000
Polyethylene Round Bottom
15.0
342082
100
18 x 100/15 B40157 (qty 2)
1
33 400
665
14 000
Bottles with Cap Assemblies
Tubes with Snap-On Caps
Polycarbonate Round Bottom
Polypropylene Round Bottom
Open-Top Tubes
Microfuge Tubes
Polypropylene tube
1.5
357448
500
11 x 38/1.5 361705 and B40155 (qty 2)
3
27 000
449
14 000
Polypropylene tube
1.5
356090
500
11 x 38/1.5 361705 and B40155 (qty 2)
3
27 000
449
14 000
Adapters for Non-Beckman Coulter Tubes/Bottlesa**
Polypropylene Conical Tube w/ Capb,c,d,e,g
Polypropylene Conical Tube w/ Capb,c,d,e,g
Polypropylene Conical Tube
w/ Tethered Caph
Spin Filtration Conicali,j
Spin Filtration Conicali,j
a.
b.
c.
d.
e.
g.
h.
i.
j.
**
50.0
15.0
5.0
———
———
———
———
———
———
———
———
———
N/A
B40156 (qty 2)
B40158 (qty 2)
———
———
———
———
———
———
———
———
———
**
**
**
50.0
15.0
———
———
———
———
———
———
N/A
B40156 (qty 2)
———
———
———
———
———
———
**
**
Use only the items listed here and observe fill volumes and maximum run speeds.
Manufactured by Greiner.
Manufactured by BD Falcon.
Manufactured by VWR.
Manufactured by Corning.
Manufactured by NUNC.
Manufactured by Eppendorf.
Manufactured by Pall.
Manufactured by Millipore.
Use manufacturer’s recommendations for tube and bottle RCF limits. The corresponding RPM and k factor can be calculated using the equations on pages 1 and 15 of the JA-24.50 rotor manual.
Rotor Replacement Parts
Supplies
B19947
B19952
885501
961696
360998
339558
335148
339555
306812
338765
1-26
Dual-Locking Lid
Single-Locking Lid
Lid O-Ring (ethylene propylene rubber)
Rotor Body O-Ring (ethylene propylene rubber)
Rotor removal tool
Rotor Cleaning Kit
Silicone vacuum grease (1 oz)
Solution 555 (1 qt)
Spinkote lubricant (2 oz)
Stem Lifter (adapter removal) tool
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JA-14
JA-14
6 x 250 mL
Fixed-Angle Rotor, Aluminum
Major applications: General-purpose, large-volume and multi-tube
processing. Adapters permit use of up to 30 each 15-mL tubes and up
to 72 each 3-mL tubes.
Axis of Rotation
rmin (35 mm)
rav (86 mm)
rmax (137 mm)
25°
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
14 000
30 100
1 764
6 x 250 mL
62 x 120 mm
2.5 x 4.8 in
1.5 L
For use in Avanti J Series centrifuges.
No. 339247. JA-14§ Fixed-Angle Biosafety Rotor for 14 000 rpm operation.
Tubes and Bottles
Tube Style/Material
Bottles with Screw-On Caps
Polypropylene
Polycarbonate
Conical Polypropylene
Conical Polycarbonate
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
50.0
50.0
230.0
230.0
357003
357002
356989
356987
25
25
6
6
29 x 104
29 x 104
60 x 120
60 x 120
356997
356997
356983
356983
1
1
1
1
30 100
30 100
8 600
8 600
1 764
1 764
6 158
6 158
14 000
14 000
7 500
7 500
50.0
50.0
50.0
250.0
250.0
357001
361694
357000
356013
356011
6
24
6
6
6
29 x 104
29 x 104
29 x 104
62 x 120
62 x 120
356997
356997
356997
———
———
1
1
1
———
———
30 100
30 100
30 100
30 100
30 100
1 764
1 764
1 764
1 764
1 764
14 000
14 000
14 000
14 000
14 000
50.0
50.0
363664
357005
25
25
29 x 104
29 x 104
356997
356997
1
1
30 100
30 100
1 764
1 764
14 000
14 000
50.0
50.0
363647
357007
25
25
29 x 104
29 x 104
356997
356997
1
1
30 100
30 100
1 764
1 764
14 000
14 000
4.0
566353
1000
14 x 55
342098*
9
20 000
———
14 000
———
———
———
———
———
———
———
———
12 x 75
13 x 100
17 x 119
29 x 104
30 x 104
30 x 104
53 x 114
60 x 120
9
8
4
1
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Bottles with Cap Assemblies
Polypropylene
Polypropylene
Polycarbonate
Wide-mouth Polycarbonate
Wide-mouth Polypropylene
Tubes with Snap-On Caps
Polycarbonate
Thickwall Polypropylene
Open-Top Tubes
Thickwall Polycarbonate
Thickwall Polypropylene
BioVials
Polypropylene
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
Round Bottom
Conical
Round Bottom
Conical
Round Bottom
Round Bottom
Conical
*
5.0
7.0
15.0
30.0
50.0
50.0
150.0
230.0
———
———
———
———
———
———
———
———
356968 (qty. of 1)
356974 (qty. of 1)
356964 (qty. of 1)
356997/870331 (qty. of 1)
356966 (qty. of 1)
356997 (qty. of 1)
339362 (qty. of 1)
356983 (qty. of 4)
Can be double-stacked.
Rotor Replacement Parts
346965
870137
870138
811656
360473
Rotor Removal Tool
Spring Pin for JA-14 Rotor Lid
O-ring for Rotor Lid
O-ring for Rotor Body
Rotor Lid Assembly (includes Tie-down Kit)
Adapters
342098
356983
356997
356995
Beckman Coulter High Performance Centrifuges
1-27
1
www.beckmancoultercentrifugation.com
JA-12
Fixed-Angle Rotor, Aluminum
Axis of Rotation
1
JA-12
12 x 50 mL
Major applications: Pelleting cells, bacteria, or subcellular fractions;
phase separations; binding studies.
rmin (71 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
rav (108 mm)
12 000*
23 200
1 244
12 x 50 mL
600 mL
30 x 103 mm (conical)
1.17 x 4 in
rmax (144 mm)
Rotor Capacity
35°
For use in Avanti J Series centrifuges.
No. 360992. JA-12§ Fixed-Angle Rotor with Dual-locking Biosafety Lid
No. 360993. JA-12 Fixed-Angle Rotor with Single-locking Lid
Tubes and Bottles
Tube Style/Material
Conical*
Nominal
Volume per
Tube (mL)
Part No.
Quantity
50.0
——
——
Cavity
Diameter
(mm)
——
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
——
——
23 200
——
12 000
1
———
———
———
Adapters for Non-Beckman Coulter Tubes/Bottles
Conical
*
15.0
Tested using Corning and Falcon 50-mL conical tubes.
———
———
17 x 119 393088 (qty. of 1)
Rotor Replacement Parts
364846
360991
360999
961696
961697
364919
364922
360998
1-28
Dual-locking Lid Assembly
Single-locking Lid Assembly
Tie-down Bolt (for use without lid)
O-ring for Rotor Body
O-ring for Rotor Lid
Tie-down Kit (Single)
Tie-down Kit (Dual-lock)
Rotor Removal Tool
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JLA-10.500
JLA-10.500
6 x 500 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
Major applications: Large-volume for initial processing of bacteria or other
cells from fermentors, clearing cell debris from homogenates, or pelleting
subcellular organelles and protein precipitates.
rmin (54 mm)
rav (110 mm)
rmax (166 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
10 000
18 600
2 850
6 x 500 mL
69 x 160 mm
2.75 x 6.5 in
3L
20°
For use in Avanti J Series centrifuges.
No. 369681. J-LITE JLA-10.500 Rotor Assembly. Includes 6 canisters with secondary Biosafety liquid closures.
No. 360828. JLA-10.500 canisters with secondary BioSafety liquid closures (set of 2).
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Cap Assemblies
Polypropylene
50.0
357001
6
29 x 104
356996
1
12 000
2 840
10 000
Polycarbonate
50.0
500.0
357000
361690
6
6
29 x 104
69x 160
356996
———
1
———
12 000
18 600
2 840
2 840
10 000
10 000
Polypropylene
500.0
361691
6
69 x 160
———
———
11 900
4 440
8 000
Wide-mouth Polycarbonate
250.0
356013
6
62 x 122
362750** (set of 2)
1
17 700
2 840
10 000
Wide-mouth Polypropylene
250.0
356011
6
62 x 122
362750** (set of 2)
1
17 700
2 840
10 000
Bottles with Screw-On Caps
Polypropylene
50.0
357003
25
29 x 104
356996
1
12 000
2 840
10 000
Polycarbonate
50.0
357002
25
29 x 104
356996
1
12 000
2 840
10 000
Polycarbonate
50.0
363664
25
29 x 104
356996
1
12 000
2 840
10 000
Polypropylene
50.0
357005
25
29 x 104
356996
1
12 000
2 840
10 000
Thickwall Polycarbonate
50.0
363647
25
29 x 104
356996
1
12 000
2 840
10 000
Thickwall Polypropylene
50.0
357007
25
29 x 104
356996
1
12 000
2 840
10 000
4.0
566353
1000
14 x 55
362750/342098**
9
———
———
10 000
———
———
———
———
———
———
12 x 75
13 x 100
17 x 100
17 x 120
25 x 105
29 x 115
356967 (qty. of 1)
356973 (qty. of 1)
356994 (qty. of 1)
356995 (qty. of 1)
359398 (qty. of 1)
356965 (qty. of 1)
11
8
5
5
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Tubes with Snap-On Caps
Open-Top Tubes
BioVials
Polypropylene
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
Round Bottom
Round Bottom
Conical
Round Bottom
Conical
4.0
7.0
15.0
15.0
30.0
50.0
———
———
———
———
———
———
*
BioVials require adapters P/N 362750 AND 342098 to run in the JLA-10.500. Two adapters P/N
342098 can be double-stacked inside of one adapter P/N 362750 per rotor cavity if greater capacity
is desired.
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Rotor Replacement Parts
363334
360828
360842
360848
363335
360834
Rotor Lid Assembly
Canister (set of 2 with 2 canister closures)
Canister Closure Assembly (1)
O-ring for Canister Closure (set of 12)
Canister Rack
Canister Sleeve Washer
Adapters
342098
356996
362750
356995
Beckman Coulter High Performance Centrifuges
1-29
1
www.beckmancoultercentrifugation.com
JA-10
JA-10
6 x 500 mL
Fixed-Angle Rotor, Aluminum
Axis of Rotation
Major applications: Large-volume for initial processing of tissue homogenates and other large particles.
rmin (38 mm)
1
rav (98 mm)
rmax (158 mm)
25°
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
10 000
17 700
3 610
6 x 500 mL
69 x 160 mm
2.75 x 6.5 in
3L
For use in Avanti J Series centrifuges.
No. 369687. JA-10 Fixed-Angle Rotor for 10 000 rpm operation.
Includes carrying handle.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Cap Assemblies
Polypropylene
50.0
357001
6
29 x 104
356996
1
12 000
———
10 000
Polycarbonate
50.0
357000
6
29 x 104
356996
1
12 000
———
10 000
Wide-mouth Polycarbonate
250.0
500.0
356013
355605
6
6
62 x 120
69 x 160
362750** (set of 2)
———
1
———
17 700
17 700
———
———
10 000
10 000
Wide-mouth Polypropylene
250.0
500.0
356011
355607
6
6
62 x 120
69 x 160
362750** (set of 2)
———
1
———
17 700
11 300
———
———
10 000
8 000
50.0
357003
25
29 x 104
356996
1
12 000
———
10 000
Polycarbonate
50.0
363664
25
29 x 104
356996
1
12 000
———
10 000
Polypropylene
50.0
357005
25
29 x 104
356996
1
12 000
———
10 000
Thickwall Polycarbonate
50.0
363647
25
29 x 104
356996
1
12 000
———
10 000
Thickwall Polypropylene
50.0
357007
25
29 x 104
356996
1
12 000
———
10 000
4.0
566353
1000
14 x 55
362750/342098**
9
———
———
10 000
———
———
———
———
———
———
12 x 75
13 x 100
17 x 100
17 x 120
25 x 105
29 x 115
356967 (qty. of 1)
356973 (qty. of 1)
356994 (qty. of 1)
356960 (qty. of 1)
359398 (qty. of 1)
356965 (qty. of 1)
11
8
5
5
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Bottles with Screw-On Caps
Polypropylene
Tubes with Snap-On Caps
Open-Top Tubes
BioVials
Polypropylene
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
Round Bottom
Round Bottom
Conical
Round Bottom
Conical
4.0
7.0
15.0
15.0
30.0
50.0
*
———
———
———
———
———
———
BioVials require adapters P/N 362750 AND 342098 to run in the JA-10. Two adapters
P/N 342098 can be double-stacked inside of one adapter P/N 362750 per rotor cavity
if greater capacity is desired.
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Rotor Replacement Parts
334827
870139
811656
364911
392122
Rotor Removal Tool
O-ring for Rotor Lid
O-ring for Rotor Body
Tie-down Kit
Rotor Lid
Adapters
342098
1-30
356996
362750
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JLA-9.1000
JLA-9.1000
4 x 1000 mL
Axis of Rotation
Fixed-Angle Rotor, Aluminum
20°
Major applications: General purpose, large-volume processing, pelleting of
bacteria, subcellular organelles, viruses, and precipitates. Empty rotor weight
is 23.9 lb (10.8 kg).
82 mm
134 mm
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
9 000
16 800
2 544
4 x 1000 mL
95 x 191 mm
3.8 x 7.65 in.
4L
rmin
rav
For use in Avanti J Series centrifuges.
rmax
185 mm
No. 366754. J-LITE JLA-9.1000 Rotor Package. Includes rotor body,
lid, carbon fiber canisters, and labware kit 392574.
No. 969330. J-LITE JLA-9.1000 Rotor Package. Includes rotor body,
lid, carbon fiber canisters, labware kit 392574, and 4 polycarbonate
bottles with cap assemblies (two A98812).
No. 969331. J-LITE JLA-9.1000 Rotor Package. Includes rotor body,
lid, carbon fiber canisters, labware kit 392574, and 4 polypropylene
bottles with cap assemblies (two A98813).
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed*
Bottles with Cap Assemblies*
J-LITE PC-1000† (Polycarbonate)
1000.0
A98812
2
95 x 191
––––––
––––––
16 800
2 544
9 000
J-LITE PP-1000‡ (Polypropylene)
with Polyphenylsufone Cap
1000.0
A98813
2
95 x 191
––––––
––––––
16 800
2 544
9 000
J-LITE PP-1000‡ (Polypropylene)
with Polyphenylene Sulfide Cap
1000.0
B29896
2
95 x 191
––––––
––––––
16 800
2 544
9 000
Polypropylene
50.0
357001
6
29 x 104
356996/363683**
1
–––––
–––––
9 000
Polycarbonate
50.0
357000
6
29 x 104
356996/363683**
1
–––––
–––––
9 000
Wide-mouth Polycarbonate
250.0
500.0
356013
355605
6
6
62 x 120
69 x 160
362750/363683**
363683
1
1
–––––
–––––
–––––
–––––
9 000
9 000
Wide-mouth Polypropylene
250.0
500.0
356011
355607
6
6
62 x 120
69 x 160
362750/363683**
363683
1
1
–––––
–––––
–––––
–––––
9 000
8 000
J-LITE PC-1000 (Polycarbonate)
1000.0
366751
2
95 x 191
––––––
––––––
16 800
2 544
9 000
J-LITE PP-1000 (Polypropylene)
1000.0
A98814
2
95 x 191
––––––
––––––
16 800
2 544
9 000
J-LITE PP-1000‡ (Polypropylene)
1000.0
B29048
12
95 x 191
––––––
––––––
16 800
2 544
9 000
50.0
357003
25
29 x 104
356996/363683**
1
–––––
–––––
9 000
Polycarbonate
50.0
363664
25
29 x 104
356996/363683**
1
–––––
–––––
9 000
Polypropylene
50.0
357005
25
29 x 104
356996/363683**
1
–––––
–––––
9 000
Bottles Only
Bottles with Screw-On Caps
Polypropylene
Tubes with Snap-On Caps
*
Bottle assemblies include cap/closure. The cap/closure serves the dual purpose of sealing the bottle
and providing a secondary seal on the carbon-fiber canister to minimize sample loss resulting from
bottle leak or failure.
† PC-1000 must be run with cap/closure; minimum fill volume is 500 mL.
‡ PP-1000 must be run with cap/closure; minimum fill volume is 1000 mL.
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Patented HarvestLine System Liners
Disposable liners provide easy-to-use,
low-cost sample containment, and
storage
Adapters
356996
362750
342098
Continued on next page.
Beckman Coulter High Performance Centrifuges
1-31
1
www.beckmancoultercentrifugation.com
JLA-9.1000 (continued)
JLA-9.1000
4 x 1000 mL
1
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed*
Open-Top Tubes
Thickwall Polycarbonate
50.0
363647
25
29 x 104 356996/363683**
1
–––––
–––––
9 000
Thickwall Polypropylene
50.0
357007
25
29 x 104 356996/363683**
1
–––––
–––––
9 000
4.0
566353
1000
1
–––––
–––––
9 000
BioVials
Polypropylene
14 x 55
342098/362750/363683**
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Labware Kit Part Number 392574
Rotor Replacement Parts
Includes: 1 ea. 974627 Bottle Rack (holds 6 bottles)
1 ea. 363646 Spatula
1 ea. 366770 Tool Kit
363583
363686
363601
B10317
B29894
Accessories
974627
363646
A99028
B29895
393898
366749
366770
366772
369264
369256
Bottle Rack (holds 6 bottles for easy transport)
Spatula
Polyphenylsufone Cap/Closure Assembly (set of 2)
(Includes cap/closure, O-ring, plug, and plug O-ring)
Polyphenylene Sulfide Cap/Closure Assembly (set of 2)
(Includes cap/closure, O-ring, plug, and plug O-ring)
Plug Assembly, AutoVent Polycarbonate (set of 2)
Plug Assembly, AutoVent polyetherimide (PEI)(set of 2)
Tool Kit (includes one bottle grip and one wrench)
Polytetrafluoroethylene (PTFE) Spray
HarvestLine System Liner Kit
(Includes 369256 one-liter bottle liners (qty 120), 368740
filling rack, 977472 funnel, and 369267 instructional CD.)
HarvestLine System Liners
Fits JLA-8.1000 or JLA-9.1000 one-liter bottles (qty. 120).
B10319
B10320
Rotor Lid Assembly
J-LITE 1000 Removable Canister (set of 2)
Canister Sleeve Washer (set of 6)
Polyphenylsufone Cap/Closure Replacement Assembly (set of 6)
Polyphenylene Sulfide Cap/Closure Replacement Assembly
(set of 6)
Cap/Closure Replacement O-rings (set of 6)
AutoVent Plug Replacement O-rings (set of 6)
Includes six large plug O-rings and six AutoVent plug
O-rings.
Adapters
356996
1-32
362750
342098
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JLA-8.1000
JLA-8.1000
6 x 1000 mL
Fixed-Angle Rotor, Aluminum
20°
Axis of Rotation
119 mm
171 mm
222.8 mm
Major applications: General purpose, large-volume processing, pelleting
of bacteria, cell organelles, viruses, and precipitates. Empty rotor weight
is 16.8 kg (37 lb).
rmin
rav
rmax
Max. RPM
Max. g
k Factor
Number of Bottles
Volume/Size
Rotor Capacity
8 000
15 970
2 482
6 x 1000 mL
95 x 191 mm
3.8 x 7.65 in.
6L
For use in Avanti J-26 XP Series centrifuges
and Avanti J-HC.
No. 363688. J-LITE JLA-8.1000 Rotor Package . Includes rotor body,
Biosafety lid, carbon fiber canisters, and labware kit 392574.
No. 969328. J-LITE JLA-8.1000 Rotor Package. Includes rotor body,
lid, carbon fiber canisters, labware kit 392574, and 6 polycarbonate
bottles with cap assemblies (three A98812).
No. 969329. J-LITE JLA-8.1000 Rotor Package. Includes rotor body,
lid, carbon fiber canisters, labware kit 392574, and 6 polypropylene
bottles with cap assemblies (three A98813).
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Cap Assemblies*
J-LITE PC-1000† (Polycarbonate)
1000.0
A98812
2
95 x 191
––––––
––––––
15 970
2 482
8 000
J-LITE PP-1000‡ (Polypropylene)
with Polyphenylsufone Cap
1000.0
A98813
2
95 x 191
––––––
––––––
15 970
2 482
8 000
J-LITE PP-1000 (Polypropylene)
with Polyphenylene Sulfide Cap
1000.0
B29896
2
95 x 191
––––––
––––––
15 970
2 482
8 000
Polypropylene
50.0
357001
6
29 x 104
356996/363683**
1
–––––
–––––
8 000
Polycarbonate
50.0
357000
6
29 x 104
356996/363683**
1
–––––
–––––
8 000
Wide-mouth Polycarbonate
250.0
500.0
356013
355605
6
6
62 x 120
69 x 160
362750/363683**
363683
1
1
–––––
–––––
–––––
–––––
8 000
8 000
Wide-mouth Polypropylene
250.0
500.0
356011
355607
6
6
62 x 120
69 x 160
362750/363683**
363683
1
1
–––––
–––––
–––––
–––––
8 000
8 000
J-LITE PC-1000 (Polycarbonate)
1000.0
366751
2
95 x 191
––––––
––––––
15 970
2 482
8 000
J-LITE PP-1000 (Polypropylene)
1000.0
A98814
2
95 x 191
––––––
––––––
15 970
2 482
8 000
J-LITE PP-1000 (Polypropylene)
1000.0
B29048
12
95 x 191
––––––
––––––
15 970
2 482
8 000
50.0
357003
25
29 x 104
356996/363683**
1
–––––
–––––
8 000
Polypropylene
50.0
363664
25
29 x 104
356996/363683**
1
–––––
–––––
8 000
Polypropylene
50.0
357005
25
29 x 104
356996/363683**
1
–––––
–––––
8 000
Bottles Only
Bottles with Screw-On Caps
Polypropylene
Tubes with Snap-On Caps
Patented HarvestLine System Liners
*
Bottle assemblies include cap/closure. The cap/closure serves the dual purpose of sealing the bottle
and providing a secondary seal on the carbon-fiber canister to minimize sample loss resulting from
bottle leak or failure.
† PC-1000 must be run with cap/closure; minimum fill volume is 500 mL.
‡ PP-1000 must be run with cap/closure; minimum fill volume is 1 000 mL.
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Disposable liners provide easy-to-use,
low-cost sample containment, and
storage
Adapters
356996
362750
342098
Continued on next page.
Beckman Coulter High Performance Centrifuges
1-33
1
www.beckmancoultercentrifugation.com
JLA-8.1000 (continued)
JLA-8.1000
6 x 1000 mL
1
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Open-Top Tubes
Thickwall Polycarbonate
50.0
363647
25
29 x 104
356996/363683**
1
–––––
–––––
8 000
Thickwall Polypropylene
50.0
357007
25
29 x 104
356996/363683**
1
–––––
–––––
8 000
4.0
566353
1000
14 x 55
342098/362750/363683**
1
–––––
–––––
9 000
BioVials
Polypropylene
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Labware Kit Part Number 392574
Includes: 1 ea. 974627 Bottle Rack (holds 6 bottles)
1 ea. 363646 Spatula
1 ea. 366770 Tool Kit
Accessories
974627
363663
363646
A99028
B29895
393898
366749
366770
366772
369264
369256
Bottle Rack (holds 6 bottles for easy transport)
Bottle Rack (holds 3 bottles for easy transport)
Spatula
Polyphenylsufone Cap/Closure Assembly (set of 2)
(Includes cap/closure, O-ring, plug, and plug O-ring)
Pohlyphenylene Sulfide Cap/Closure Assembly (set of 2)
(Includes cap/closure, O-ring, plug, and plug O-ring)
Plug Assembly, AutoVent Polycarbonate (set of 2)
Plug Assembly, AutoVent polyetherimide (PEI) (set of 2)
Tool Kit (includes one bottle grip and one wrench)
Polytetrafluoroethylene (PTFE) Spray
HarvestLine System Liner Kit (includes 369256 one-liter
bottle liners (qty. 120), P/N 368740 filling rack, P/N 977472
funnel, and P/N 369267 instructional CD.)
HarvestLine System Liners (fits JLA-8.1000 or JLA-9.1000
one-liter bottles (qty. 120).
Rotor Replacement Parts
363563
363686
363601
B10317
B29894
B10319
B10320
Rotor Lid Assembly
J-LITE Removable Canister (set of 2)
Canister Sleeve Washer (set of 6)
Polyphenylsufone Cap/Closure Replacement Assembly
(set of 6)
Polyphenylene Sulfide Cap/Closure Replacement Assembly
(set of 6)
Cap/Closure Replacement O-rings (set of 6)
AutoVent Plug Replacment O-rings (set of 6)
(Includes six large plug O-rings and six AutoVent plug
O-rings)
Adapters
356996
1-34
362750
342098
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-24.38
JS-24.38
6 x 38.5 mL
Swinging-Bucket Rotor, Aluminum, Titanium Buckets
Major applications: Harvesting bacteria, processing tissue homogenates,
subcellular particulates, routine pelleting such as precipitates and phase
separations.
Axis of Rotation
rmax (161.0 mm)
rav (118.2 mm)
rmin (75.3 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
24 000*
103 900
334
6 × 38.5 mL
25 × 89 mm
1 × 3.5 in
231 mL
For use in Avanti J Series centrifuges
(except Avanti J-E).
No. 360743. JS-24.38 Swinging-Bucket Rotor, with six 38.5-mL buckets.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed*
Quick-Seal Bell-Top Tubes
Polypropylene
15.0
27.0
33.0
343664
343665
344623
50
50
50
25 x 38
25 x 64
25 x 83
355536†
355536†
355536†
1
1
1
103 900
103 900
103 900
167
260
317
24 000
24 000
24 000
8.5
358652
50
25 x 38
358156
355536†
1
103 900
167
24 000
Polypropylene
38.5
326823
50
25 x 89
——
——
103 900
334
24 000
konical Polypropylene
25.0
30.0
358125
358126
50
50
25 x 76
25 x 89
358156
358156
1
1
95 500
103 900
297
334
24 000
24 000
konical Polypropylene
Open-Top Tubes
Thickwall Polypropylene
32.0
355642
25
25 x 89
——
——
103 900
334
24 000
Thickwall Polycarbonate
32.0
355631
25
25 x 89
——
——
103 900
334
24 000
Ultra-Clear
38.5
344058
50
25 x 89
——
——
103 900
334
24 000
*
†
Reaches 24 000 rpm in the Avanti J-30I centrifuge only. Not recommended for use in Avanti J-25
Series (max. speed 10 000 rpm), or Avanti J-26 XP Series (max. speed 10 000 rpm). Incompatible
with all J2 Series.
Modified polyphenylene oxide Floating Spacer.
Rotor Replacement Parts
362397
812715
362785
Bucket Set - 38 mL (set of 6 with caps and O-rings)
Bucket O-ring
Rotor Stand
Adapters
355536
358156
Beckman Coulter High Performance Centrifuges
1-35
1
www.beckmancoultercentrifugation.com
JS-24.15
JS-24.15
6 x 15 mL
Swinging-Bucket Rotor, Aluminum, Titanium Buckets
Major applications: Harvesting bacteria, processing tissue homogenates,
subcellular particulates, routine pelleting such as precipitates and phase
separations.
Axis of Rotation
rmax (171.3 mm)
rav (125.2 mm)
1
rmin (79 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
24 000*
110 500
376
6 × 15 mL
16 × 96 mm
0.625 × 3.75 in
90 mL
For use in Avanti J Series centrifuges
(except Avanti J-E).
No. 362396. JS-24.15 Swinging-Bucket Rotor with six 15-mL buckets.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed*
355579†
355579†
355579†
355579†
1
1
1
1
110 500
110 500
110 500
110 500
193
215
261
293
24 000
24 000
24 000
24 000
Quick-Seal Bell-Top Tubes
Polypropylene
4.2
6.3
8.0
10.0
356562
345830
344621
344622
50
50
50
50
16 x 38
16 x 45
16 x 58
16 x 67
Open-Top Tubes
Polypropylene
15.0
361707
50
16 x 96
——
——
110 500
376
24 000
konical Polypropylene
12.5
361708
50
16 x 93
358155
1
108 500
368
24 000
Ultra-Clear
15.0
361706
50
16 x 96
——
——
110 500
376
24 000
*
†
Reaches 24 000 rpm in the Avanti J-30I centrifuge only. Not recommended for use in Avanti J-25
Series (max speed 10 000 rpm), or Avanti J-26 XP Series (max. speed 10 000 rpm). Incompatible
with all J2 Series.
Modified polyphenylene oxide Floating Spacer.
Rotor Replacement Parts
362398
815472
362785
364802
Bucket Set - 15 mL (set of 6 with caps and O-rings)
Bucket O-ring
Rotor Stand
Rotor and Adapter Assembly
Adapters
358155
1-36
355579
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-13.1
JS-13.1
6 x 50 mL
Swinging-Bucket Rotor, Aluminum
Major applications: Harvesting bacteria, processing tissue homogenates,
separating cell particulates. Isolation of cell membrane using density
gradients.
Axis of Rotation
rmin (41 mm)
rav (91 mm)
rmax (140 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
13 000
26 500
1 841
6 x 50 mL
29 x 105 mm
1.125 x 4 in
300 mL
90°
For use in Avanti J Series centrifuges.
No. 346963. JS-13.1 Swinging-Bucket Rotor for 13 000 rpm operation.
Includes carrying handle. Tubes and bottles not included.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polypropylene
50.0
357003
25
29 x 104
———
———
26 500
1 840
13 000
Polycarbonate
10.0
50.0
355672
357002
25
25
16 x 80
29 x 104
342327/870329
———
1
———
26 500
26 500
1 840
1 840
13 000
13 000
Polypropylene
50.0
357005†
25
29 x 104
———
———
26 500
1 840
13 000
Polycarbonate
50.0
363664†
25
29 x 104
———
———
26 500
1 840
13 000
Polyethylene
1.8
340196
500
11 x 39
344497*
3
26 500
1 840
13 000
Tubes with Snap-On Caps
Polypropylene
Natural
1.5
357448
500
11 x 38
344497*
3
26 500
1 840
13 000
Polypropylene
Orange
Yellow
Green
Blue
Natural
1.5
1.5
1.5
1.5
1.5
356094
356093
356092
356091
356090
500
500
500
500
500
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
344497*
344497*
344497*
344497*
344497*
3
3
3
3
3
26 500
26 500
26 500
26 500
26 500
1 840
1 840
1 840
1 840
1 840
13 000
13 000
13 000
13 000
13 000
10.0
355630
25
16 x 76
342327/870329
4/1
8 000
———
13 000
———
———
———
———
12 x 75
13 x 100
18 x 80
29 x 104
356970 (qty. of 1)
356976 (qty. of 1)
870329 (qty. of 1)
870331 (qty. of 1)
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
Open-Top Tubes
Thickwall Polycarbonate
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
*
†
5.0
8.0
15.0
30.0
———
———
———
———
Can be double-stacked. Force will vary depending on tube position.
Cannot be run with caps in this rotor.
Rotor Replacement Parts
346965
346976
364862
364921
346979
346964
Rotor Removal Tool
Buckets (set of 6)
Rotor Tie-down Assembly
Tie-down Kit
Windshield Bowl Trim
Rotor Lid Assembly
Adapters
342327
344497
870329
Beckman Coulter High Performance Centrifuges
1-37
1
www.beckmancoultercentrifugation.com
JS-7.5
Swinging-Bucket Rotor, Aluminum
Axis of Rotation
Major applications: Initial processing of cells and removal of cell debris
from culture media.
rmin (51 mm)
rav (108 mm)
1
JS-7.5
4 x 250 mL
rmax (165 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
7 500
10 400
5 287
4 x 250 mL
62 x 136 mm
2.5 x 5.5 in
1L
90°
For use in Avanti J Series centrifuges
(except Avanti J-E).
No. 336380. JS-7.5 Swinging-Bucket Rotor for 7 500 rpm operation.
Includes four 250-mL buckets. Multitube carriers available separately.
Tubes and bottles not included.
No. 362212. JS-7.5 Rotor without buckets.
Multitube Carriers
For added versatility, three Multitube Carriers are available for the JS-7.5 Rotor. These Carriers require no adapters to accommodate 50-mL conical
tubes, 50-mL round-bottom tubes, and 5-mL round-bottom tubes. Multitube carriers are interchangeable with the buckets of the JS-7.5 Rotor.
50-mL Conical Tube Carrier
No. 362213. Multitube Carrier for 50-mL conical
tubes. Holds three tubes per carrier
for maximum rotor capacity of 12 × 50-mL
conical tubes. Set of two.
Major applications: General-purpose pelleting (especially of cells), as
well as nucleic acid precipitations and isolation of mononuclear cells
using Ficoll-Paque.
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
7 500
10 580
4 140
12 x 50 mL
(conical)
29 x 103 mm
1.125 x 4 in
600 mL
Major applications: General-purpose centrifugation.
50-mL Round-Bottom Tube Carrier
No. 362214. Multitube Carrier for 50-mL roundbottom tubes. Holds four tubes per
carrier for maximum rotor capacity of
16 × 50-mL tubes. Set of two.
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
7 500
10 080
3 959
16 x 50 mL
29 x 104 mm
1.125 x 4 in
800 mL
Tubes for 250-mL Bucket
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polycarbonate
250.0
355673
6
62 x 136
336389
1
10 400
5 290
7 500
Polypropylene
50.0
357003
25
29 x 104
356997
1
9 000
———
7 500
50.0
50.0
355603
357000
6
6
16 x 76
29 x 104
356997
356997
1
1
9 000
9 000
———
———
7 500
7 500
Conical Polycarbonate
230.0
356987
6
62 x 141
356983
1
10 400
5 290
7 500
Wide-mouth Polycarbonate
250.0
356013
6
62 x 120
———
———
10 400
5 290
7 500
Conical Polypropylene
230.0
356989
6
62 x 141
356983
1
10 400
5 290
7 500
Wide-mouth Polypropylene
250.0
356011
6
62 x 120
———
———
10 400
5 290
7 500
Bottles with Cap Assemblies
Polycarbonate
1-38
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-7.5 (continued)
JS-7.5
4 x 250 mL
Tubes for 250-mL Bucket (continued)
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Tubes with Snap-On Caps
Polycarbonate
50.0
363664
25
29 x 104
356997
1
9 000
———
7 500
Polypropylene
50.0
357005
25
29 x 104
356997
1
9 000
———
7 500
Polycarbonate (Graduated)
50.0
363647
25
29 x 104
356997
———
10 080
5 290
7 500
Polypropylene (Graduated)
50.0
357007
25
29 x 104
356997
4
9 000
———
7 500
4.0
566353
1000
14 x 55
342098*
9
———
———
7 500
Quantity
Size
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Open Top Tubes
BioVials
Polypropylene
Tubes for 4-place Carrier
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Maximum
Speed
Bottles with Screw-On Caps
Polypropylene
50.0
357003
25
29 x 104
356997
1
10 400
———
7 500
Polycarbonate
50.0
357002
25
29 x 104
356997
1
10 400
———
7 500
Polycarbonate
50.0
363664**
25
29 x 104
———
———
10 400
———
7 500
Polypropylene
50.0
357005**
25
29 x 104
———
———
10 400
———
7 500
50.0
363647
25
29 x 104
———
———
10 400
———
7 500
356968
356974
356964
356995
870329
870331
356997/
870331
356996
356997
339362
9
8
4
5
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
1
1
1
———
———
———
———
———
———
———
———
———
Tubes with Snap Caps
Open-Top Tubes
Thickwall Polycarbonate
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
Round Bottom
Conical
Round Bottom
Round Bottom
Round Bottom
Round Bottom
5.0
7.0
15.0
15.0
15.0
30.0
30.0
———
———
———
———
———
———
———
1
1
1
1
1
1
1
12 x 75
13 x 199
17 x 118
18 x 98
18 x 80
29 x 104
29 x 103
Conical
Round Bottom
Round Bottom
50.0
50.0
150.0
———
———
———
1
1
1
30 x 104
30 x 104
53 x ___
* Adapter 342098 can be double-stacked to increase rotor capacity.
** See Rotor Manual for instructions on positioning these tubes in the carrier.
Rotor Replacement Parts
885367
816952
362216
362212
Retaining Ring for Rotor Knob Shaft if purchased after 1/1/81.
If purchased before 1/1/81, use 885367 and 336747.
Polyethylene Rotor Foot
250-mL Buckets (set of 2)
JS-7.5 Rotor without carriers or buckets
364920
362213
362214
362215
Tie-down Kit
3 x 50 mL Conical Multitube Carriers (set of 2)
4 x 50 mL Round-bottom Multitube Carriers (set of 2)
24 x 5 mL Round-bottom Multitube Carriers (set of 2)
Adapters
336389
342098
356964
356983
Beckman Coulter High Performance Centrifuges
356997
1-39
1
www.beckmancoultercentrifugation.com
JS-5.9
JS-5.9
10 Microplates
Swinging-Bucket Rotor, Aluminum
Major applications: High-throughput processing (DNA or RNA kits),
serial dilution of small volumes.
1
rmin
138.6 mm
rav
153.4 mm
rmax
168.5 mm
Max. RPM
Max. g
Rotor
Capacity
Approximate
Accel/Decel Time
(sec)
5 900
6 570
10 Microplates
47/49
For use in Avanti J-26S Series and Avanti J-30I centrifuges.
Axis of Rotation
No. 369331. JS-5.9 Swinging-Bucket Rotor Assembly. Two-place rotor
with removable aluminum swinging buckets (set of two), microplate carriers (set of two), support pad for microplates (set of four), and tie-down.
Microplates
Plate or Tube Style/Material
Nominal
Volume per
Well
Multiwell Plate, Polystyrene, Nonsterile
300 µL
609844
100
96 wells
——
——
5 900
1 mL
267001
24
96 wells
——
——
5 900
Deep-well Plate, Polystyrene, Nonsterile*
Part No.
Quantity
No. of
Wells
g-Force
k Factor
Maximum
Speed
Deep-well Plate, Polystyrene, Sterile*
1 mL
267004
24
96 wells
——
——
5 900
Deep-well Plate, Polypropylene, Nonsterile
1 mL
267006
24
96 wells
——
——
5 900
Deep-well Plate, Polypropylene, Sterile
1 mL
267007
24
96 wells
——
——
5 900
Square-well Plate, Polypropylene
2 mL
140504
24
96 wells
——
——
5 900
Accessories
Rotor Replacement Parts
267002
267005
538619
368957
538618
339558
339555
339379
977212
368919
368920
Cap Strip, Nonsterile† (pkg. of 12)
Cap Strip, Sterile† (pkg. of 12)
Aluminum Foil Lid‡ (pkg. of 100)
Support Pad for Microplates (set of 4)
Rubber Roller, 4-inch, for sealing microplate lids
Rotor Cleaning Kit
Beckman Coulter Solution 555 (1 qt)
Rotor Cleaning Brush
Paint On Graphite Lubricant (1/2 oz)
Bucket (set of 2)
Microplate Carrier (set of 2)
* Do not stack these plates.
† Caps are optional.
‡ Requires 4-inch soft rubber roller (538618) for installation.
1-40
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-5.3 AllSpin
◊
JS-5.3 AllSpin
24 Microplates
Swinging-Bucket Rotor, Anodized Aluminum
Major applications: Rapid sedimentation of protein precipitates, large
particles, cells, and cell debris. It can be used for binding studies and
separating serum from whole blood.
rmin
rav
rmax
179.6 mm
194.8 mm
Axis of Rotation
rmin
rav
92.7 mm
155.6 mm
rmax
Max. RPM
Max. g
Number of Tubes
Volume
Rotor Capacity
5 300
5 300
6 870
6 130
4 x 500 mL
24 microplates
8 deep-well
microplates
2L
2L
164.3 mm
218.4 mm
For use in Avanti J-E and J-26S centrifuges only.
Axis of Rotation
No. 368690. JS-5.3 Swinging-Bucket Rotor. Four-place rotor with
anodized aluminum buckets designed for use with adapters to
accommodate a wide range of tubes and bottles. Rotor buckets are
interchangeable with microplate carriers. Includes tie-down knob.
No. 969314. JS-5.3 Swinging-Bucket Rotor Package. Includes rotor,
buckets, tie-down knob, and microplate carrier kit (368914).
Microplate Carrier Kit
No. 368914. Microplate Carrier Kit. Includes 4 carriers (368905) and
4 support pads (369382).
Replacement Parts for Microplate Carriers
369382
368914
368417§
368706
Support Pads (set of 4)
Microplate Carrier (set of 4)
Aeroseal Covers for JS-5.3 AllSpin Rotor (set of 2)
Bucket Upgrade Kit (set of 4 blue buckets)***
Tubes and Bottles
Size
Required
Adapter
(pkg. 4)
Adapter
Color
Tubes per
Adapter
Maximum
Speed
g-Force*
5 mL round, 13 mm diameter, variable lengths
392071
Beige
33
6 145
5 300
10 mL round, 16 mm diameter, variable lengths
392072
Purple
24
6 145
5 300
15 mL round, 17 mm diameter, variable lengths
392073
Red
20
6 145
5 300
15 mL conical, 17 mm diameter, variable lengths
392075
Green
18
6 425†
5 300
50 mL round, 29 mm diameter, variable lengths
392074
Yellow
8
6 145
5 300
50 mL conical, 30 × 115 mm
392076
Black
7
6 480†
5 300
250 mL round/230 mL conical
392077
Yellow
1
6 670
5 300‡
250 mL conical, 60 × 172 mm
392079
Orange
1
6 870
5 300
500 mL conical £
392078
Blue
1
6 870
5 300
15 mL conical
356964 (each)
Yellow
4
6 670
5 300**
50 mL conical
356966 (each)
Yellow
1
6 670
5 300**
*
Maximum RCF and speed for adapters. Use manufacturers’ recommendations for tube
and bottle limits.
** Use this adapter inside the 250-mL adapter (392077)
*** Rotors with black buckets require Bucket Upgrade Kit 368706
†
Maximum RCF for labware tested in this adapter is 4 000 × g. Use manufacturers’
recommendations for max g-force.
‡
Requires a cushion (356983) to be placed at bottom of adapter.
◊
£
When used with optional Aeroseal Covers, P/N 368417.
Cannot be used with bucket cover
Continued on next page.
Adapters
392071
392072
392073
392074
Beckman Coulter High Performance Centrifuges
392075
392076
392079
392077
392078
356966
356964
1-41
1
www.beckmancoultercentrifugation.com
JS-5.3 AllSpin (continued)
JS-5.3 AllSpin
4 x 500 mL
1
Description
Nominal Volume
per Well
Part No.
Quantity
Maximum
Speed
Accessory
Description*
Part No.
Quantity
300 µL
609844
100
5 300
Cap Strip, Nonsterile†
Cap Strip, Sterile†
Aluminum Foil Lid‡
267002
267005
538619
12
12
100
Deep-Well Polystyrene Plate,
96-Well, Nonsterile
1 mL
267001
24
5 300
Cap Strip, Nonsterile
Cap Strip, Sterile
Aluminum Foil Lid
267002
267005
538619
12
12
100
Deep-Well Polystyrene Plate,
96-Well, Sterile
1 mL
267004
24
5 300
Cap Strip, Nonsterile
Cap Strip, Sterile
Aluminum Foil Lid
267002
267005
538619
12
12
100
Deep-Well Polypropylene Plate,
96-Well, Nonsterile
1 mL
267006
24
5 300
Cap Strip, Nonsterile
Cap Strip, Sterile
Aluminum Foil Lid
267002
267005
538619
12
12
100
Deep-Well Polypropylene Plate,
96-Well, Sterile
1 mL
267007
24
5 300
Cap Strip, Nonsterile
Cap Strip, Sterile
Aluminum Foil Lid
267002
267005
538619
12
12
100
Square-Well Polystyrene Plate,
2 mL
140504
24
5 300
Aluminum Foil Lid
538619
100
Microplates
Multiwell Polystyrene Plate,
96-Well, Nonsterile
*
†
‡
When stacking polypropylene multiwell plates, place a support pad (369382) beneath the bottom
plate and a cap strip between the plates to prevent breakage during centrifugation. Also use the
support pad beneath all polystyrene deep-well plates.
Caps are optional.
Requires 4-inch soft-rubber roller (538618) for installation.
Rotor Replacement Parts
368690
368410
368706
368914
JS-5.3 rotor assembly
Rotor tie-down knob
Bucket (set of 4)
Microplate carrier kit
Contains:
368905 Microplate carrier (set of 4)
369382 Support pad (set of 4)
Rotor Supplies
Replacement tube and bottle adapters (set of 2):
368907 Beige (13 mm dia)
368909 Purple (16 mm dia)
368910 Red (17 mm dia)
368911 Yellow (29 mm dia)
368915 Green (15 mL conical)
368916 Black (50 mL conical)
369385 Orange (250 mL conical)
369383 Yellow (250 mL round/230 mL conical)
369384 Blue (500 mL conical)
538618 Rubber Roller, 4-in., for sealing foil microplate lids
1-42
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-5.2
*
JS-5.2
4 x 1000 mL
Swinging-Bucket Rotor (Windshielded),
Anodized Aluminum
Axis of
Rotation
rmin (86 mm)
rav (156 mm)
Max. RPM
Max. g
Rotor
Capacity
Approximate
Accel/Decel Time
(min:sec)
5 200
6 840
4 Liters, 4 Blood Bags,
12 Microplates,
148 RIA-Tubes
2:30/3:00
rmax (226 mm)
For use in J6-MI centrifuge.
No. 339087. JS-5.2§ Swinging-Bucket Biosafety Rotor. Windshielded,
four-place rotor with aluminum rotor yoke and removable aluminum
swinging buckets. Buckets are interchangeable with Microplate Carriers
for spinning microtiter plates and MiniTube Racks.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polycarbonate
10.0
50.0
250.0
500.0
750.0
1000.0
355672
357002
355673
355664
358299
355675
25
25
6
6
6
6
16 x 80
29 x 104
62 x 136
69 x 160
96 x 130
97 x 167
341977
339103
339108
339109
356096 (set of 6)
356096 (set of 6)
19
7
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
5 200
5 200
5 200
5 200
5 200
5 200
Polypropylene
50.0
100.0
500.0
750.0
1000.0
357003
355624
355665
356855
355676
25
6
6
6
6
29 x 104
38 x 102
69 x 159
96 x 130
97 x 167
339103
339104
339109
356096 (set of 6)
356096 (set of 6)
7
2
1
1
1
———
———
———
———
———
———
———
———
———
———
5 200
5 200
5 200
5 200
5 200
Polypropylene
50.0
361694
6
29 x 104
356997
1
———
———
5 200
Polypropylene
50.0
357001
6
29 x 104
339103
7
———
———
5 200
Polycarbonate
50.0
70.0
357000
355620
6
6
29 x 104
38 x 102
339103
339104
7
2
———
———
———
———
5 200
5 200
Wide-mouth Polycarbonate
250.0
500.0
356013
355605
6
6
62 x 120
69 x 160
339108
339109
1
1
———
———
———
———
5 200
5 200
Wide-mouth Polypropylene
250.0
500.0
356011
355607
6
6
62 x 120
69 x 160
339108
339109
1
1
———
———
———
———
5 200
5 200
70.0
500.0
355655
355649
6
6
———
———
———
———
5 200
5 200
Bottles with Cap Assemblies
Bottles with Screw-On Caps
Polycarbonate
38 x 102
69 x 160
339104
339109
2
1
Wide-mouth Polycarbonate
250.0
358275
25
62 x 122
339108
1
———
———
5 200
Polypropylene
100.0
500.0
355626
355650
6
6
38 x 102
69 x 159
339104
339109
2
1
———
———
———
———
5 200
5 200
Wide-mouth Polypropylene
250.0
358326
25
62 x 122
339108
1
———
———
5 200
Conical Polycarbonate
230.0
356987
6
60 x 120
356983/339108**
1
———
———
5 200
Conical Polypropylene
230.0
356989
6
60 x 120
356983/339108**
1
———
———
5 200
* When used with optional Aeroseal Covers, P/N 343686.
** These adapters fit inside one another to accommodate smaller bottles and tubes.
§ This rotor was tested to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when used and
maintained as instructed. Validation of microbiological containment was done at an independent, third-party facility (CAMR, Porton Down, UK or USAMRIID, Ft. Detrick, MD,
USA). Improper use or maintenance may affect seal integrity and, thus, containment.
Continued on next page.
Adapters
339100
339101
339102
339103
339104
Beckman Coulter High Performance Centrifuges
339108
339109
341977
354511
356096
356983
1-43
1
www.beckmancoultercentrifugation.com
JS-5.2 (continued)
JS-5.2
4 x 1000 mL
1
Tubes and Bottles (continued)
Tube Style/Material
Nominal
Volume per
Tube (mL)
Part No.
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
26
26
26
26
26
26
26
———
———
———
———
———
———
———
———
———
———
———
———
———
———
5 200
5 200
5 200
5 200
5 200
5 200
5 200
Tubes with Snap-On Caps
Polypropylene
Natural
Orange
Yellow
Green
Blue
Natural
Natural*
1.5
1.5
1.5
1.5
1.5
1.5
1.5
357448
356094
356093
356092
356091
356090
343169
500
500
500
500
500
500
500
Polyethylene
1.8
340196
500
11 x 39
339100/354511**
26
———
———
5 200
Polycarbonate
50.0
363664
25
29 x 104
339103
7
———
———
5 200
Polypropylene
50.0
357005
25
29 x 104
339103
7
———
———
5 200
Thickwall Polypropylene
10.0
355640
25
16 x 76
341977
19
———
———
5 200
Thickwall Polycarbonate
10.0
15.0
50.0
355630
342080
363647
25
100
25
16 x 76
18 x 98
29 x 104
341977
339102
356997
19
14
7
———
———
———
———
———
———
5 200
5 200
5 200
Thickwall Polyethylene
15.0
342081
100
18 x 98
339102
14
———
———
5 200
Thickwall Polypropylene
15.0
50.0
342082
357007
100
25
18 x 98
29 x 104
339102
339103
14
7
———
———
———
———
5 200
5 200
4.0
566353
1000
14 x 55
339101
24
———
———
5 200
339119 (qty. of 1)
(double stacked
adapter)
339100 (qty. of 1)
339101 (qty. of 1)
341977 (qty. of 1)
356995 (qty. of 1)
339102 (qty. of 1)
345386 (qty. of 1)
341794 (qty. of 1)
19
———
———
———
37
24
19
5
14
4
4
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Open-Top Tubes
BioVials
Polypropylene
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
3.0 & 5.0
———
———
12 x 75
Round Bottom
Round Bottom
Round Bottom
Conical
Round Bottom
Conical
Round Bottom
3.0 & 5.0
10.0
12.0
15.0
20.0
50.0
50.0
———
———
———
———
———
———
———
———
———
———
———
———
———
———
12 x 133
14 x 133
16 x 133
17 x 119
18 x 133
30 x 136
35 x 136
* Cap separate.
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Adapters
339100
1-44
339101
339102
339103
339104
339108
339109
341977
354511
356096
356983
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-5.2 (continued)
JS-5.2
4 x 1000 mL
1
Blood-Bag Cups
No. 339127. Yellow cup with inner diameter of 88 mm for single- and
double-packs. Set of four.
No. 339129. Red cup with inner diameter of 98 mm for triple- and
quad-packs. Sold individually.
Aeroseal Covers §
No. 343686. Cover for round buckets of JS-5.2. Features O-ring seal to
provide added aerosolprotection. Transparent so broken tubes can be
detected and proper precautions taken before breaking seal. Quantity 1.
Microplate Carriers
No. 358680. Special carriers, interchangeable with buckets, slip onto
yoke of JS-5.2 Rotor. Each carrier holds three microplates for a total
capacity of 12 per run. Maximum speed 2 600 rpm (1450 x g). Set of
two.
§ Validation of microbiological containment was done at an independent, third-party facility (CAMR,
Porton Down, UK or USAMRIID, Ft. Detrick, MD, USA). Improper use or maintenance may affect
seal integrity and, thus, containment.
Beckman Coulter High Performance Centrifuges
Rotor Supplies
339031
341710
878439
367045
Rotor Tie-down Screw
Bucket Set (set of 4)
Torquing Bar for Rotor Tie-down Screw
Rotor Tie-down Kit for Avanti J-26 XP Series centrifuge
1-45
www.beckmancoultercentrifugation.com
JS-5.0
JS-5.0
4 x 2.25 L
Swinging-Bucket Rotor, Anodized Aluminum
Major applications: Separating bacterial, yeast, and tissue homogenates;
harvesting cultures.
1
r min
(108 mm)
rav
(188 mm)
rmax
(267 mm)
Max. RPM
Max. g
k Factor
Number of
Containers
Rotor Capacity
5 000
7 480
9 171
4 x 2.25 L
9L
For use in Avanti J-HC centrifuges.
Axis of Rotation
No. 367820. JS-5.0 Swinging-Bucket Rotor Assembly with Labware Kit.
Four-place rotor with anodized aluminum buckets. Includes 4 cups, 4 cup
covers with plugs, 8 red cup gaskets, 4 green liner gaskets, 60 air vent
filters, 50 HarvestLine System Liners, 2 cup racks, 1 spatula.
Labware Kit
No. 368732. Includes 4 cups, 4 cup covers with plugs, 8 red cup gaskets,
4 green liner gaskets, 60 air vent filters, 50 HarvestLine System Liners,
2 cup racks, 1 spatula.
Container Assembly
No. 368730. Includes 4 cups, 4 covers with plugs, and 4 cup gaskets
(369257).
HarvestLine System Liners
No. 368735. Set of 50 polyethylene system liners (food grade compliant).
Rotor Accessories
Rotor Replacement Parts
368727
367891
369259
367837
369257
369261
346965
367824
368521
369258
1-46
Rack
Spatula (set of 2)
Partition (set of 4)
Buckets (set of 4)
Gaskets, Cup (red, set of 4)
Gaskets, Liner (green, set of 4)
Rotor Removal Tool
Tie-down Bolt
Tie-down Tool
Plug, cover, set of 4
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-4.3
**
JS-4.3
4 x 750 mL
Swinging-Bucket Rotor, Anodized Aluminum
Major applications: Rapid sedimentation of protein precipitates, large
particles, cells, and cell debris. It can be used for binding studies and
separating serum from whole blood.
Axis of Rotation
rmin (86 mm)
rav (145 mm)
rmax (204 mm)
Max. RPM
Max. g
k Factor
Number of Tubes
Volume/Size
Rotor Capacity
3 250
4 220
11 800
4 x 750 mL
96 x 130 mm
3.8 x 5.2 in
3L
For use in Avanti J-26 XP Series.
Aerosolve Canisters which are designed to provide added protection from
aerosols. Rotor buckets are interchangeable with microplate carriers.
No. 362734. JS-4.3 Swinging-Bucket Biosafety Rotor. Four-place rotor
with anodized aluminum buckets designed for use with modular disk
adapters to accommodate a variety of tubes (as indicated in the chart below)
and other adapters and blood bag cups. Can be used with Beckman Coulter
Bucket Covers
No. 361264. Set of two. Transparent bucket covers for JS-4.3 Buckets
to contain broken tubes. Includes gasket and clips.
Aerosolve Canisters
No. 359232. Set of four.
No. 359481. Set of two.
Aerosolve Canisters fit in JS-4.3 Rotor Buckets. These canisters feature
an O-ring seal and are completely transparent so a broken tube can be seen
and proper precautions taken before the seal is broken. Canisters can
also be used as 500-mL wide-mouth bottles. Specially-designed adapters
accommodate most popular tubes within the canister (see table at end of
this rotor section).
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Set of 2/Set of 4
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
359474/359153
359486/359164
349946 (ea.)
349846 (set of 6)
7
4
1
1
3 830
3 670
4 040
4 040
———
———
———
———
4 300
4 300
4 300
4 300
359474/359153
359486/359164
349945 (ea.)
349846 (set of 6)
7
4
1
1
3 830
———
4 300
4 150
4 040
———
———
4 300
4 300
Bottles with Screw-On Caps
Polycarbonate
50.0
357002
25
29 x 104
250.0
750.0
355673
358299
6
6
62 x 136
96 x 130
50.0
357003
25
29 x 104
500.0
750.0
355665
356855
6
6
69 x 159
96 x 130
Conical Polycarbonate
230.0
356987
6
62 x 141
356983/349946*
356985 (ea.)
1
4 040
———
4 300
Conical Polypropylene
230.0
356989
6
62 x 141
356983/349946*
356985 (ea.)
1
4 040
———
4 300
Wide-mouth Polypropylene
250.0
356011
6
62 x 122
349946 (ea.)
1
4 040
———
4 300
Wide-mouth Polycarbonate
250.0
356013
6
62 x 122
349946 (ea.)
1
4 040
———
4 300
Polypropylene
** Use adapter 356983 with adapter 349946.
** When used with optional Aerosolve Canisters, P/N 359232 and 359481.
Continued on next page.
Adapters
344517
349846
349945
349946
354495
354511
Beckman Coulter High Performance Centrifuges
356983
356985
359469
359470
359471
359473
359474
359484
359486
1-47
1
www.beckmancoultercentrifugation.com
JS-4.3 (continued)
1
JS-4.3
4 x 750 mL
MicroPlus Carriers
Replacement Parts for MicroPlus Carrier
No. 362394. MicroPlus Carrier Assembly. Includes carrier, base, and
rubber pad to cushion plates. Each carrier can hold up to 3 microplates,
1 deep-well/square well plate, 1 rack of MiniTubes, or other labware in 96well format. Set of 2. Maximum allowable speed is 3,250 rpm (1924 x g).
361302
361291
361304
Base and Rubber Pads (set of 2)
Rubber Pads (set of 4)
MicroPlus Carriers (set of 2)
Tubes and Bottles (continued)
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Set of 2/Set of 4
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
359474/359153
359486/359164
359474/359153
359486/359164
7
4
7
4
———
———
4 300
———
———
———
———
4 300
4 300
359474/359153
359486/359164
359474/359153
359486/359164
7
4
7
4
———
———
4 300
———
———
———
———
4 300
4 300
Bottles with Cap Assemblies
Polypropylene
50.0
357001
6
29 x 104
50.0
361694
6
29 x 104
50.0
357000
6
29 x 104
50.0
361693
24
29 x 104
Wide-mouth Polypropylene
500.0
355607
6
69 x 160
349945 (ea.)
1
———
———
4 300
Wide-mouth Polycarbonate
250.0
358275
25
62 x 122
349946 (ea.)
1
———
———
4 300
Wide-mouth Polypropylene
250.0
358326
25
62 x 120
349946 (ea.)
1
———
———
4 300
500.0
355650
6
69 x 159
349945 (ea.)
1
———
———
4 300
Thickwall Polypropylene
10.0
355640
25
16 x 76
359471/359150
359484/359162
19
12
———
———
4 300
Thickwall Polycarbonate
10.0
355630
25
16 x 76
———
4 300
342080
363664
100
25
18 x 98
29 x 104
19
12
14
7
4
———
15.0
50.0
359471/359150
359484/359162
359473/359152
359474/359153
359486/359164
———
———
———
———
4 300
4 300
Polycarbonate
Bottles
Polypropylene
Open-Top Tubes
Thickwall Polyethylene
15.0
342081
100
18 x 98
359473/359152
14
———
———
4 300
Thickwall Polypropylene
15.0
50.0
342082
357007
100
25
18 x 98
29 x 104
359473/359152
359474/359153
359486/359164
14
7
4
———
———
———
———
4 300
4 300
Graduated Polycarbonate
50.0
363075
8
29 x 104
359474/359153
359486/359164
7
4
———
———
4 300
1.5
357448
500
11 x 38
359469/359148
354511 (ea.)*
354495 (ea.)
26
———
———
4 300
1.8
340196
500
11 x 39
359469/359148
354511 (ea.)*
354495 (ea.)
26
———
———
4 300
50.0
357005
25
29 x 104
359474/359153
359486/359164
7
4
———
———
4 300
Natural†
1.5
343169
500
11 x 38
26
———
———
4 300
Natural†
1.5
356090
500
11 x 38
359469/359148
354511 (ea.)*
354495 (ea.)
359469/359148
354511 (ea.)*
354495 (ea.)
26
26
———
———
4 300
Tubes with Snap-On Caps
Polypropylene
Polyethylene
White
Polypropylene
26
26
Adapters
344517
1-48
349846
349945
349946
354495
354511
356983
356985
359469
359470
359471
359473
359474
359484
359486
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-4.3 (continued)
JS-4.3
4 x 750 mL
Tubes and Bottles (continued)
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Set of 2/Set of 4
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
26
———
———
4 300
Tubes with Snap-On Caps (continued)
Polypropylene
Blue
1.5
356091
500
11 x 38
Green
1.5
356092
500
11 x 38
Yellow
1.5
356093
500
11 x 38
Orange
1.5
356094
500
11 x 38
359469/359148
354511 (ea.)*
354495 (ea.)
359469/359148
354511 (ea.)*
354495 (ea.)
359469/359148
354511 (ea.)*
354495 (ea.)
359469/359148
354511 (ea.)*
354495 (ea.)
26
26
———
———
4 300
26
26
———
———
4 300
26
26
———
———
4 300
359470/359149
344517 (ea.)
24
10
———
———
4 300
26
BioVials
Polypropylene
4.0
566353
1 000
14 x 55
Aerosolve Canisters
Part No.
Aerosolve Canisters for JS-4.3
343686
Aerosolve Canister Adapters
Color
Code
Typical Tube/Bottle
Volume (mL)
Maximum Tube
Diameter (mm)
Tubes per
Adapter
Tubes per JS-4.3
Rotor
Adapter Assembly
Part No. (set of 4)
Adapter Assembly
Part No. (set of 2)
1.5
3 and 5
5
10
12
3 and 5
15
15 and 20
3 and 5
15
3 and 5
50
3 and 5
50
3 and 5
11
12
13
14
16
12
14
18
12
17
12
30
12
29
12
24
24
24
18
12
6
10
12
6
6
6
4
4
4
4
96
96
96
72
48
24
40
48
24
24
24
16
16
16
16
354495
359482
359489
359483
359484
––––––
359160
358993
359161
359162
344517
359485
––––––
359163
359487
358991
359488
358992
359486
359164
Orange
230
62
1
4
–––
356985
Canister Kit
500
–––
–––
–––
359481
359232
White
Blue
Tan
Orange
Purple
White (vials)
Green
Light Green
(conical)
Lime Green
(conical)
Yellow
*
†
Rotor Replacement Parts
Tube retainer (P/N 354511) is sold separately.
Cap separate.
361261
361251
361264
361304
361302
362737
Aluminum Buckets, weight-matched set of 4
Rotor Yoke, forged stainless steel
Bucket Cover Kit
MicroPlus Multiwell Plate Carriers, with bases and pads (set of 2)
Replacement Bases for MicroPlus Carriers, with pads (set of 2)
Knob Assembly
Adapters
344517
349846
349945
349946
354495
354511
Beckman Coulter High Performance Centrifuges
356983
356985
359469
359470
359471
359473
359474
359484
359486
1-49
1
www.beckmancoultercentrifugation.com
JS-4.2
*
Swinging-Bucket Rotor (Windshielded),
Anodized Aluminum
Axis of
Rotation
1
JS-4.2
6 x 1000 mL
Major applications: Rapid sedimentation of protein, rotor material,
precipitates, large particles, cells, and cell debris. It can be used for
binding studies and separating serum from whole blood.
rmin (114 mm)
rav (184 mm)
rmax (254 mm)
Max. RPM
Max. g
Capacity
Rotor
Approximate
Accel/Decel Time (min:sec)
2 500
5 020
6 Liters, 6 Blood Bags,
18 Microplates,
336 RIA-Tubes
2:30/3:00
For use in Avanti J-HC and J6-MI centrifuges only.
No. 339080. JS-4.2§ Swinging-Bucket Biosafety Rotor. Windshielded, sixplace rotor with aluminum rotor yoke and removable aluminum swinging
buckets. Buckets are interchangeable with Microplate Carriers 358682‡
for spinning microtiter plates and MiniTube Racks.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polycarbonate
10.0
50.0
250.0
500.0
750.0
1000.0
355672
357002
355673
355664
358299‡
355675
25
25
6
6
6
6
16 x 80
29 x 104
62 x 136
69 x 160
96 x 130
97 x 167
341977
339103
339108
339109
356096 (set of 6)
356096 (set of 6)
19
7
1
1
1
1
5 020
5 020
5 020
5 020
5 020
5 020
———
———
———
———
———
———
4 200
4 200
4 200
4 200
4 200
4 200
Polypropylene
50.0
500.0
750.0
1000.0
357003
355665
356855**
355676**
25
6
6
6
29 x 104
339103
69 x 159
339109
96 x 130 356096 (set of 6)
97 x 167 356096 (set of 6)
7
1
1
1
5 020
5 020
5 020
5 020
———
———
———
———
4 200
4 200
4 200
4 200
Teflon
50.0
363076
8
28.5 x 107
339103
7
5 020
———
4 200
Wide-mouth Polycarbonate
250.0
500.0
356013
355605
6
6
62 x 120
69 x 160
339108
339109
1
1
5 020
5 020
———
———
4 200
4 200
Wide-mouth Polypropylene
250.0
500.0
356011
355607
6
6
62 x 120
69 x 160
339108
339109
1
1
5 020
5 020
———
———
4 200
4 200
Conical Polycarbonate
230.0
356987*
6
62 x 120 356983(4-pk)/339108$
1
5 020
———
4 200
Conical Polypropylene
230.0
356989*
6
62 x 141 356983(4-pk)/339108
1
5 020
———
4 200
Polypropylene
50.0
50.0
357001
361694
6
6
29 x 104
29 x 104
339103
356997
7
1
5 020
5 020
———
———
4 200
4 200
Polycarbonate
50.0
70.0
357000
355620
6
6
29 x 104
38 x 102
339103
339104
7
2
5 020
5 020
———
———
4 200
4 200
Polypropylene
100.0
355624
6
38 x 102
339104
2
5 020
———
4 200
70.0
500.0
355655
355649
6
6
38 x 102
69 x 160
339104
339109
2
1
5 020
5 020
———
———
4 200
4 200
$
Bottles with Cap Assemblies
Bottles
Polycarbonate
Wide-mouth Polycarbonate
250.0
358275
25
62 x 122
339108
1
5 020
———
4 200
Polypropylene
100.0
500.0
355626
355650
6
6
38 x 102
69 x 159
339104
339109
2
1
5 020
5 020
———
———
4 200
4 200
Wide-mouth Polypropylene
250.0
358326
25
62 x 120
339108
1
5 020
———
4 200
Tubes and Bottles (continued)
*
†
When used with optional Aeroseal Covers, P/N 343686.
Adapter used in Aerosolve Canister (P/N 359232).
** These bottles require a sleeve rather than an adapter.
$
These adapters fit inside one another to accommodate smaller bottles and tubes.
Adapters
339100
1-50
339101
339102
339103
339104
339108
339109
341977
354511
356096
356983
356997
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-4.2 (continued)
Tube Style/Material
JS-4.2
6 x 1000 mL
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Tubes with Snap-On Caps
Polypropylene
Natural
Polycarbonate
1.5
357448
500
11 x 38
339100/354511**
26
5 020
———
4 200
50.0
363664
25
29 x 104
356997
1
5 020
———
4 200
Polyethylene
Natural
1.8
340196
500
11 x 39
339100/354511**
26
5 020
———
4 200
Polypropylene
Natural‡
Natural
Blue
Green
Yellow
Orange
1.5
1.5
1.5
1.5
1.5
1.5
50.0
343169
356090
356091
356092
356093
356094
357005
500
500
500
500
500
500
25
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
29 x 104
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339103
26
26
26
26
26
26
7
5 020
5 020
5 020
5 020
5 020
5 020
5 020
———
———
———
———
———
———
———
4 200
4 200
4 200
4 200
4 200
4 200
4 200
4.0
566353
1 000
14 x 55
339101
24
5 020
———
4 200
BioVials
Polypropylene
Open-Top Tubes
Polypropylene
10.0
355640
25
16 x 76
341977
19
5 020
———
4 200
Polycarbonate
10.0
15.0
50.0
355630
342080
363647
25
100
25
16 x 76
18 x 98
29 x 104
341977
339102
339103
19
14
7
5 020
5 020
5 020
———
———
———
4 200
4 200
4 200
Polyethylene
15.0
342081
100
18 x 98
339102
14
5 020
———
4 200
Polypropylene
15.0
50.0
342082
357007
100
25
18 x 98
29 x 104
339102
339103
14
7
5 020
5 020
———
———
4 200
4 200
*
‡
** These adapters fit inside one another to accommodate smaller bottles and tubes.
These tubes cannot be run with bucket covers in place.
Cap separate.
Blood-Bag Cups
No. 339127. Yellow cup with inner diameter of 88 mm for singleand double-packs. Set of two.
No. 339129. Red cup with inner diameter of 98 mm for tripleand quad-packs. Sold individually.
Aeroseal Covers§
No. 343686. Cover for round buckets of JS-4.2. Features O-ring seal to
provide added aerosol protection. Transparent so broken tubes can be
detected and proper precautions taken before breaking seal. Quantity 1.
Microplate Carriers
No. 358682. Special carriers, interchangeable with buckets, slip onto
yoke of JS-4.2 Rotor. Each carrier holds three microplates for a total
capacity of 18 per run. Max. speed 2 500 rpm, max RCF 1 500 x g.
Set of two.
§
Validation of microbiological containment was done at an independent, third-party facility (CAMR,
Porton Down, UK or USAMRIID, Ft. Detrick, MD, USA). Improper use or maintenance may affect
seal integrity and, thus, containment.
Rotor Replacement Parts
367045
348392
368575
339096
349817
Rotor Tie-down Kit
Cover Assembly
Buckets (qty: 6, blue-anodized)
Bail for Adapter (curved arm at top)
Bail for Adapter (straight arm at top)
Adapters
339100
339101
339102
339103
339104
Beckman Coulter High Performance Centrifuges
339108
339109
341977
354511
356096
356983
356997
1-51
1
www.beckmancoultercentrifugation.com
JS-4.2A
**
JS-4.2A
6 x 1000 mL
Swinging-Bucket Rotor (Windshielded),
Anodized Aluminum
Axis of Rotation
Major applications: Rapid sedimentation of protein precipitates, large
particles, cells, and cell debris. It can be used for binding studies and
separating serum from whole blood.
1
Max. RPM
Max. g
Rotor
Capacity
Approximate
Accel/Decel Time
(min:sec)
2 500
5 020
6 Liters, 6 Blood Bags,
18 Microplates,
336 RIA-Tubes
2:30/3:00
rmin
(114 mm)
rav
(184 mm)
rmax
(254 mm)
For use in J6-MI centrifuges only.
No. 366695. JS-4.2A§ Swinging-Bucket Biosafety Rotor. Windshielded,
six-place rotor with aluminum rotor yoke and removable aluminum
swinging buckets. Buckets are interchangeable with Microplate Carriers
358682† for spinning microtiter plates and MiniTube Racks. ARIES
“Smart Balance” technology provides imbalance compensation for rotors
with buckets that are unbalanced up to 100 grams.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polycarbonate
10.0
50.0
750.0
1000.0
355672
357002
358299*
355675*£
25
25
6
6
16 x 80
341977
29 x 104
339103
96 x 130 356096 (set of 6)
97 x 167 356096 (set of 6)
19
7
1
1
5 020
5 020
5 020
5 020
———
———
———
———
4 200
4 200
4 200
4 200
Polycarbonate
250.0
500.0
355673
355664
6
6
62 x 136
69 x 160
1
1
5 020
5 020
———
———
4 200
4 200
Polypropylene
500.0
750.0
1000.0
355665
356855*
355676* £
6
6
6
69 x 159
339109
96 x 130 356096 (set of 6)
97 x 167 356096 (set of 6)
1
1
1
5 020
5 020
5 020
———
———
———
4 200
4 200
4 200
339108
339109
Wide-mouth Polycarbonate
250.0
250.0
500.0
358275
356013
355605
25
6
6
62 x 122
62 x 120
69 x 160
339108
339108
339109
1
1
1
5 020
5 020
5 020
———
———
———
4 200
4 200
4 200
Wide-mouth Polypropylene
250.0
250.0
500.0
358326
356011
355607
25
6
6
62 x 122
62 x 120
69 x 160
339108
339108
339109
1
1
1
5 020
5 020
5 020
———
———
———
4 200
4 200
4 200
Conical Polycarbonate
230.0
356987
6
60 x 120
356983/339108+
1
5 020
———
4 200
Conical Polypropylene
230.0
356989
6
60 x 120
356983/339108+
1
5 020
———
4 200
Polycarbonate
70.0
500.0
355655
355649
6
6
38 x 102
69 x 160
339104
339109
2
1
5 020
5 020
———
———
4 200
4 200
Polypropylene
50.0
100.0
500.0
357003
355626
355650
25
6
6
29 x 104
38 x 102
69 x 159
339103
339104
339109
7
2
4
5 020
5 020
5 020
———
———
———
4 200
4 200
4 200
Polypropylene
50.0
50.0
357001
361694
6
6
29 x 104
29 x 104
339103
356997
7
1
5 020
5 020
———
———
4 200
4 200
Polycarbonate
50.0
70.0
357000
355620
6
6
29 x 104
38 x 102
339103
339104
7
2
5 020
5 020
———
———
4 200
4 200
Polypropylene
100.0
355624
6
38 x 102
339104
2
5 020
———
4 200
Bottles
Bottles with Cap Assemblies
Microplate carriers cannot be run in the Avanti J-HC centrifuge.
** These bottles require a sleeve rather than an adapter.
** When used with optional Aeroseal Covers, P/N 343686
‡
+
£
These adapters fit inside one another to accommodate smaller bottles and tubes.
can’t be used with the Aeroseal covers
Adapters
339103
1-52
339104
339108
339109
341977
356096
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-4.2A (continued)
JS-4.2A
6 x 1000 mL
Tubes and Bottles (continued)
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
339100/354511**
26
5 020
———
Maximum
Speed
Tubes with Snap-On Caps
Polypropylene
Natural
1.5
Polycarbonate
357448
500
11 x 38
4 200
50.0
363664
25
29 x 104
356997
1
Polyethylene
Natural
1.5
340196
500
11 x 39
339100/354511**
26
5 020
———
4 200
4 200
Polypropylene
Natural
Natural
Blue
Green
Yellow
Orange
1.5
1.5
1.5
1.5
1.5
1.5
50.0
356090
343169
356091
356092
356093
356094
357005
500
500
500
500
500
500
25
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
29 x 104
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339103
26
26
26
26
26
26
7
5 020
5 020
5 020
5 020
5 020
5 020
5 020
———
———
———
———
———
———
———
4 200
4 200
4 200
4 200
4 200
4 200
4 200
4.0
566353
1 000
14 x 55
339101
24
5 020
———
4 200
BioVials
Polypropylene
Open-Top Tubes
Polypropylene
10.0
355640
25
16 x 76
341977
19
5 020
———
4 200
Polycarbonate
10.0
15.0
50.0
355630
342080
363647
25
100
25
16 x 76
18 x 98
29 x 104
341977
339102
356997
19
14
7
5 020
5 020
5 020
———
———
———
4 200
4 200
4 200
Polyethylene
15.0
342081
100
18 x 98
339102
14
5 020
———
4 200
Polypropylene
15.0
50.0
342082
357007
100
25
18 x 98
29 x 104
339102
339103
14
7
5 020
5 020
———
———
4 200
4 200
** hese adapters fit inside one another to accommodate smaller bottles and tubes.
Blood-Bag Cups
No. 339127. Yellow cup with inner diameter of 88 mm for singleand double-packs. Sold individually.
No. 339129. Red cup with inner diameter of 98 mm for tripleand quad-packs. Sold individually.
Aeroseal Covers§
No. 343686. Cover for round buckets of JS-4.2A. Features O-ring seal
to provide added aerosol protection. Transparent so broken tubes can be
detected and proper precautions taken before breaking seal. Quantity 1.
Microplate Carriers
No. 358682. Special carriers, interchangeable with buckets, slip onto
yoke of JS-4.2A Rotor. Each carrier holds three microplates for a total
capacity of 18 per run. Max. speed 2 500 rpm. Set of two.
§
Validation of microbiological containment was done at an independent, third-party facility (CAMR,
Porton Down, UK or USAMRIID, Ft. Detrick, MD, USA). Improper use or maintenance may affect
seal integrity and, thus, containment.
Rotor Replacement Parts
367045
348392
368575
339096
349817
Rotor Tie-down Kit
Cover Assembly
Buckets (qty: 6, blue-anodized)
Bail for Adapter (curved arm at top)
Bail for Adapter (straight arm at top)
Adapters
339100
339101
339102
339103
Beckman Coulter High Performance Centrifuges
339108
339109
354511
356983
356997
1-53
1
www.beckmancoultercentrifugation.com
JS-4.2SM
JS-4.2SM
6 x Quad Blood Bag Packs
Swinging-Bucket Rotor (Windshielded),
Anodized Aluminum
Axis of
Rotation
1
rmin (116 mm)
rav (182 mm)
rmax (248 mm)
Major applications: Separation of serum or plasma from whole blood,
blood component separation.
Max. RPM
Max. g
Rotor
Capacity
Approximate
Accel/Decel Time
(min:sec)
2 500
4 900
6 Blood Bags
18 Microplates
2:30/3:00
For use in J6-MI centrifuge only.
No. 348394. JS-4.2SM Swinging-Bucket Rotor. Windshielded, six-place
rotor designed with oval-shaped buckets specifically for blood bank
applications which utilize similarly shaped blood bags. Buckets are
interchangeable with microplate carriers for blood serum studies.
Includes six oval-shaped blood-bag cups.
Microplate Carriers
No. 358682. Special carriers, interchangeable with buckets,
slip onto yoke of JS-4.2SM Rotor. Each carrier holds three microplates
for a total capacity of 18 per run. Maximum speed 2 500 rpm. Set of two.
Blood-Bag Cups
No. 363651. Oval-shaped gray cup for quad-packs (filters). Set of two.
Rotor Replacement Parts
367045
348392
348393
348359
1-54
Rotor Tie-down Kit
Cover Assembly
Buckets (set of 6)
Liners
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-4.2SMA
JS-4.2SMA
6 x Quad Blood Bag Packs
Swinging-Bucket Rotor (Windshielded),
Anodized Aluminum
Axis of
Rotation
rmin (116 mm)
rav (182 mm)
rmax (248 mm)
Major applications: Separation of serum or plasma from whole blood,
blood component separation.
Max. RPM
Max. g
Rotor
Capacity
Approximate
Accel/Decel Time
(min:sec)
2 500
4 900
6 Blood Bags
2:30/3:00
For use in J6-MI centrifuge only.
No. 366670. JS-4.2SMA Swinging-Bucket Rotor. Windshielded, sixplace rotor designed with oval-shaped buckets specifically for blood bank
applications which utilize similarly shaped blood bags. Buckets are
interchangeable with microplate carriers for blood serum studies.
Includes six oval-shaped blood-bag cups. ARIES “Smart Balance”
technology provides imbalance compensation for rotors with buckets
that are unbalanced up to 100 grams.
Microplate Carriers
No. 358682. Special carriers, interchangeable with buckets, slip onto
yoke of JS-4.2SMA Rotor. Each carrier holds three microplates for
a total capacity of 18 per run. Maximum speed 2 500 rpm. Set of two.
Blood-Bag Cups
No. 363651. Oval-shaped gray cup for quad-packs (filters). Set of two.
Rotor Replacement Parts
367045
348392
348393
348359
Beckman Coulter High Performance Centrifuges
Rotor Tie-down Kit
Cover Assembly
Buckets (set of 6)
Liners
1-55
1
www.beckmancoultercentrifugation.com
JS-4.0
*
JS-4.0
4 x 1000 mL
Swinging-Bucket Rotor (Unshielded), Aluminum
Major applications: Rapid sedimentation of protein precipitates, large
particles, cells, and cell debris. It can be used for binding studies and
separating serum or plasma from whole blood.
Axis of
Rotation
1
rmin (86 mm)
rav (156 mm)
rmax (226 mm)
Max. RPM
Max. g
Rotor
Capacity
Approximate
Accel/Decel Time
4 000
4 050
4 Liters, 4 Blood Bags,
12 Microplates,
148 RIA Tubes
2:00/1:30 min.
For use in Avanti J-26S and J6-MI centrifuges.
No. 339086. JS-4.0 Swinging-Bucket Biosafety Rotor. Unshielded,
four-place rotor with aluminum rotor yoke and removable aluminum
swinging buckets. Buckets are interchangeable with Microplate Carriers
for spinning microtiter plates and MiniTube Racks.
Note: requires tie-down kit P/N 367045 for use with Avanti J-26S
Series centrifuges.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polycarbonate
10.0
50.0
250.0
500.0
750.0
1000.0
355672
357002
355673
355664
358299
355675
25
25
6
6
6
6
16 x 80
29 x 104
62 x 136
69 x 160
96 x 130
97 x 167
341977
339103
339108
339109
356096 (set of 6)
356096 (set of 6)
19
N/A
1
1
1
1
———
———
———
———
———
———
———
———
———
———
———
———
4 000
4 000
4 000
4 000
4 000
4 000
Polypropylene
50.0
500.0
750.0
1000.0
357003
355665
356855
355676
25
6
6
6
29 x 104
69 x 159
96 x 139
97 x 167
339103
339109
356096 (set of 6)
356096 (set of 6)
7
1
1
1
———
———
———
———
———
———
———
———
4 000
4 000
4 000
4 000
Wide-mouth Polycarbonate
250.0
500.0
356013
355605
6
6
62 x 120
69 x 160
339108
339109
1
1
———
———
———
———
4 000
4 000
Wide-mouth Polypropylene
250.0
500.0
356011
355607
6
6
62 x 120
69 x 160
339108
339109
1
1
———
———
———
———
4 000
4 000
Conical Polycarbonate
230.0
356987
6
60 x 120
356983/339108**
1
———
———
4 000
Conical Polypropylene
230.0
356989
6
60 x 120
356983/339108**
1
———
———
4 000
Polypropylene
50.0
50.0
357001
361694
6
6
29 x 104
29 x 104
339103
356997
N/A
1
———
———
———
———
4 000
4 000
Polycarbonate
50.0
70.0
357000
355620
6
6
29 x 104
38 x 102
339103
339104
N/A
2
———
———
———
———
4 000
4 000
Polypropylene
100.0
355624
6
38 x 102
339104
2
———
———
4 000
70.0
500.0
355655
355649
6
6
38 x 102
69 x 160
339104
339109
2
1
———
———
———
———
4 000
4 000
Bottles with Cap Assemblies
Bottles
Polycarbonate
Wide-mouth Polycarbonate
250.0
358275
25
62 x 122
339108
1
———
———
4 000
Polypropylene
100.0
500.0
355626
355650
6
6
38 x 102
69 x 159
339104
339109
2
1
———
———
———
———
4 000
4 000
Wide-mouth Polypropylene
250.0
358326
25
62 x 122
339108
1
———
———
4 000
* When used with optional Aeroseal Covers P/N 343686.
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Adapters
339100
1-56
339101
339102
339103
339104
339108
339109
341977
354511
356096
356983
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-4.0 (continued)
JS-4.0
4 x 1000 mL
1
Tubes and Bottles (continued)
Tube Style/Material
Nominal
Volume per
Tube (mL)
Part No.
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Tubes with Snap-On Caps
Polypropylene
Natural
Polycarbonate
Polyethylene
1.5
357448
500
11 x 38
339100/354511**
26
———
———
4 000
50.0
363664
25
29 x 104
365997
7
———
———
4 000
1.8
340196
500
11 x 39
339100/354511**
26
———
———
4 000
1.5
1.5
1.5
1.5
1.5
1.5
50.0
356094
356093
356092
356091
356090
343169
357005
500
500
500
500
500
500
25
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
29 x 104
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339103
26
26
26
26
26
26
7
———
———
———
———
———
———
———
———
———
———
———
———
———
———
4 000
4 000
4 000
4 000
4 000
4 000
4 000
Polypropylene
10.0
355640
25
16 x 76
341977
19
———
———
4 000
Polycarbonate
10.0
15.0
50.0
355630
342080
363647
25
100
25
16 x 76
18 x 98
29 x 104
341977
339102
339103
19
14
7
———
———
———
———
———
———
4 000
4 000
4 000
Polypropylene
Orange
Yellow
Green
Blue
Clear
Natural*
Open-Top Tubes
Polyethylene
15.0
342081
100
18 x 100
339102
14
———
———
4 000
Polypropylene
15.0
50.0
342082
357007
100
25
18 x 98
29 x 104
339102
356997
14
7
———
———
———
———
4 000
4 000
4.0
566353
1000
14 x 55
339101
24
———
———
4 000
19
———
———
———
37
24
19
5
14
4
4
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
BioVials
Polypropylene
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
3.0 & 5.0
———
———
Round Bottom
Round Bottom
Round Bottom
Conical
Round Bottom
Conical
Round Bottom
3.0 & 5.0
10.0
12.0
15.0
20.0
50.0
50.0
———
———
———
———
———
———
———
———
———
———
———
———
———
———
12 x 75 339119 (double stacked)
adapter) (qty. of 1)
12 x 133 339100 (qty. of 1)
14 x 133 339101 (qty. of 1)
16 x 133 341977 (qty. of 1)
17 x 119 356995 (qty. of 1)
18 x 133 339102 (qty. of 1)
30 x 136 345386 (qty. of 1)
35 x 136 341794 (qty. of 1)
* Cap separate.
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Adapters
339100
339101
339102
339103
339104
Beckman Coulter High Performance Centrifuges
339108
339109
341977
354511
356096
356983
1-57
www.beckmancoultercentrifugation.com
JS-4.0 (continued)
JS-4.0
4 x 1000 mL
1
Blood-Bag Cups
No. 339127. Yellow cup with inner diameter of 88 mm for single- and
double-packs. Sold individually.
No. 339129. Red cup with inner diameter of 98 mm for triple- and
quad-packs. Sold individually.
Aeroseal Covers
No. 343686. Cover for round buckets of JS-4.0. Features O-ring seal to
provide added aerosol protection. Transparent so broken tubes can be
detected and proper precautions taken before breaking seal. Quantity 1.
Microplate Carriers
No. 358680. Special carriers, interchangeable with buckets, slip onto
yoke of JS-4.0 Rotor. Each carrier holds three microplates for a total
capacity of 12 per run. Maximum speed 2 600 rpm (1450 x g). Set
of two.
Rotor Replacement Parts
339031
341710
878439
367045
1-58
Rotor Tie-down Screw
Bucket Set (set of 4)
Torquing Bar for Rotor Tie-down Screw
Rotor Tie-down Kit for Avanti J-26S XP Series centrifuges
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-3.0
**
JS-3.0
6 x 1000 mL
Swinging-Bucket Rotor (Unshielded),
Anodized Aluminum
Major applications: Rapid sedimentation of protein precipitates, large
particles, cells, and cell debris. It can be used for binding studies and
separating serum from whole blood.
Axis of
Rotation
rmin (114 mm)
rav (184 mm)
rmax (254 mm)
Max. RPM
Max. g
Rotor
Capacity
Approximate
Accel/Decel Time
(min:sec)
3 000
2 560
6 Liters, 6 Blood Bags,
18 Microplates,
336 RIA-Tubes
2:00/1:30 min.
For use in J6-MI centrifuge only.
No. 339081. JS-3.0§ Swinging-Bucket Biosafety Rotor. Unshielded, sixplace rotor with aluminum rotor yoke and removable aluminum swinging
buckets. Buckets are interchangeable with Microplate Carriers 358682
for spinning microtiter plates and MiniTube Racks in the JS-3.0 Rotor.
Tubes and Bottles
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Bottles with Screw-On Caps
Polycarbonate
10.0
50.0
250.0
500.0
750.0
1000.0
355672
357002
355673
355664
358299
355675
25
25
6
6
6
6
16 x 80
341977
29 x 104
339103
62 x 136
339108
69 x 160
339109
96 x 130 356096* (set of 6)
97 x 167 356096* (set of 6)
19
7
1
1
1
1
2 560
2 560
2 560
2 560
2 560
2 560
———
———
———
———
———
———
3 000
3 000
3 000
3 000
3 000
3 000
Polypropylene
50.0
500.0
750.0
1000.0
357003
355665
356855
355676
25
6
6
6
29 x 104
339103
69 x 159
339109
96 x 130 356096* (set of 6)
97 x 167 356096* (set of 6)
7
1
1
1
2 560
2 560
2 560
2 560
———
———
———
———
3 000
3 000
3 000
3 000
Wide-mouth Polycarbonate
250.0
500.0
356013
355605
6
6
62 x 122
69 x 160
339108
339109
1
1
2 560
2 560
———
———
3 000
3 000
Wide-mouth Polypropylene
250.0
500.0
356011
355607
6
6
62 x 120
69 x 160
339108
339109
1
1
2 560
2 560
———
———
3 000
3 000
Conical Polycarbonate
230.0
356987
6
60 x 120
356983/339108+
1
2 560
———
3 000
Conical Polypropylene
230.0
356989
6
60 x 120
356983/339108+
1
2 560
———
3 000
Polypropylene
50.0
50.0
357001
361694
6
6
29 x 104
29 x 104
339103
356997
7
1
2 560
5 020
———
———
3 000
4 200
Polycarbonate
50.0
70.0
357000
355620
6
6
29 x 104
38 x 102
339103
339104
7
2
2 560
2 560
———
———
3 000
3 000
Polypropylene
100.0
355624
6
38 x 102
339104
2
2 560
———
3 000
70.0
500.0
355655
355649
6
6
38 x 102
69 x 160
339104
339109
2
1
2 560
2 560
———
———
3 000
3 000
Bottles with Cap Assemblies
Bottles
Polycarbonate
Wide-mouth Polycarbonate
250.0
358275
25
62 x 122
339108
1
2 560
———
3 000
Polypropylene
100.0
500.0
355626
355650
6
6
38 x 102
69 x 159
339104
339109
2
1
2 560
2 560
———
———
3 000
3 000
Wide-mouth Polypropylene
250.0
358326
25
62 x 122
339108
1
2 560
———
3 000
354511
356096
+
These adapters fit inside one another to accommodate smaller bottles and tubes.
Adapters
339100
339101
339102
339103
339104
Beckman Coulter High Performance Centrifuges
339108
339109
341977
356983
356997
1-59
1
www.beckmancoultercentrifugation.com
JS-3.0 (continued)
JS-3.0
6 x 1000 mL
1
Tubes and Bottles (continued)
Tube Style/Material
Nominal
Part No.
Volume per
Tube (mL)
Quantity
Cavity
Diameter
(mm)
Required
Adapters
Tubes per
Adapter
g-Force
k Factor
Maximum
Speed
Tubes with Snap-On Caps
Polypropylene
Natural
Polycarbonate
1.5
357448
500
11 x 38
339100/354511**
26
2 560
———
3 000
50.0
363664
25
29 x 104
356997
1
2 560
———
3 000
Polyethylene
Natural
1.8
340196
500
11 x 39
339100/354511**
26
2 560
———
3 000
Polypropylene
Natural*
Clear
Blue
Green
Yellow
Orange
1.5
1.5
1.5
1.5
1.5
1.5
50.0
343169
356090
356091
356092
356093
356094
357005
500
500
500
500
500
500
25
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
11 x 38
29 x 104
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339100/354511**
339103
26
26
26
26
26
26
7
2 560
2 560
2 560
2 560
2 560
2 560
2 560
———
———
———
———
———
———
———
3 000
3 000
3 000
3 000
3 000
3 000
3 000
4.0
566353
1 000
14 x 55
339101
24
2 560
———
3 000
BioVials
Polypropylene
Open-Top Tubes
Polypropylene
10.0
355640
25
16 x 76
341977
19
2 560
———
3 000
Polycarbonate
10.0
15.0
50.0
355630
342080
363647
25
100
25
16 x 76
18 x 98
29 x 104
341977
339102
356997
19
14
7
2 560
2 560
2 560
———
———
———
3 000
3 000
3 000
Polyethylene
15.0
342081
100
18 x 98
339102
14
2 560
———
3 000
Polypropylene
15.0
50.0
342082
357007
100
25
18 x 98
29 x 104
339102
339103
14
7
2 560
2 560
———
———
3 000
3 000
19
———
———
———
37
24
19
5
14
4
4
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
———
Adapters for Non-Beckman Coulter Tubes/Bottles
Round Bottom
3.0 & 5.0
———
———
Round Bottom
Round Bottom
Round Bottom
Conical
Round Bottom
Conical
Round Bottom
3.0 & 5.0
10.0
12.0
15.0
20.0
50.0
50.0
———
———
———
———
———
———
———
———
———
———
———
———
———
———
12 x 75 339119 (double stacked)
adapter) (qty. of 1)
12 x 133 339100 (qty. of 1)
14 x 133 339101 (qty. of 1)
16 x 133 341977 (qty. of 1)
17 x 119 356995 (qty. of 1)
18 x 133 339102 (qty. of 1)
30 x 136 345386 (qty. of 1)
35 x 136 341794 (qty. of 1)
** These adapters fit inside one another to accommodate smaller bottles and tubes.
Adapters
339100
1-60
339101
339102
339103
339104
339108
339109
341977
354511
356096
356983
356997
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JS-3.0 (continued)
JS-3.0
6 x 1000 mL
1
Blood-Bag Cups
No. 339127. Yellow cup with inner diameter of 88 mm for single- and
double-packs. Sold individually.
No. 339129. Red cup with inner diameter of 98 mm for triple- and
quad-packs. Sold individually.
Aeroseal Covers
No. 343686. Cover for round buckets of JS-3.0. Features O-ring seal to
provide added aerosol protection. Transparent so broken tubes can be
detected and proper precautions taken before breaking seal. Quantity 1.
Microplate Carriers
No. 358682. Special carriers, interchangeable with buckets, slip onto
yoke of JS-3.0 Rotor. Each carrier holds three microplates for a total
capacity of 18 per run. Max. speed 2 500 rpm, max RCF 1 500 x g.
Set of two.
Rotor Replacement Parts
367045
368575
Beckman Coulter High Performance Centrifuges
Rotor Tie-down Kit
Buckets (set of 6, blue-anodized)
1-61
www.beckmancoultercentrifugation.com
JCF-Z
JCF-Z
How To Order the JCF-Z Rotor
1
For a complete Beckman Coulter JCF-Z Rotor system, the following
components are required.
1. A JCF-Z Rotor (choose according to application; continuous flow,
zonal, or reorienting gradient).
2. Optional high-flow seal assembly for processing up to 100 liters/hour.
3. An appropriate bracket assembly (for Avanti J Series instruments only).
4. A pump system (not supplied by Beckman Coulter, but recommendations
below).
5. A Beckman Coulter Avanti J Series centrifuge (excluding J-E).
Continuous-Flow Rotors
No. 335140. JCF-Z Continuous-Flow Rotor, Titanium, Standard Pellet Core.
Includes Standard continuous-flow core (pellet size 400 mL), standardflow rotating seal assembly, and tool kit. Bracket kit required for use with
Avanti J Series centrifuges, and pump system required for all applications.
Max. RPM
Max. g
k Factor
Rotor Capacity
20 000
39 900
100
660 mL
No. 357544. JCF-Z Continuous-Flow Rotor, Titanium, Small Pellet Core.
Includes Small Pellet continuous-flow core (pellet size 200 mL),
standard-flow rotating seal assembly, and tool kit. Bracket kit required
for use with Avanti J Series centrifuges, and pump system required for all
applications.
Max. RPM
Max. g
k Factor
Rotor Capacity
20 000
36 300
281
240 mL
No. 357521. JCF-Z Continuous-Flow Rotor, Titanium, Large Pellet Core.
Includes Large Pellet continuous-flow core (pellet size 800 mL),
standard-flow rotating seal assembly, and tool kit. Bracket kit required
for use with Avanti J Series centrifuges, and pump system required for all
applications.
Max. RPM
Max. g
k Factor
Rotor Capacity
20 000
39 900
293
1 250 mL
Bracket Kits for use with Avanti J-Series centrifuges
Zonal and Reorienting Gradient Rotors
No. 354006. JCF-Z Zonal Rotor, Titanium.
Includes Zonal core, standard-flow rotating seal assembly, and tool kit.
Bracket kit required for use with Avanti J Series centrifuges, and pump
system required for all applications.
Max. RPM
Max. g
k Factor
Rotor Capacity
20 000
39 900
710
1 900 mL
No. 354005. JCF-Z Reorienting Gradient Rotor, Titanium.
Includes reorienting gradient core, sample transfer assembly, and tool kit.
Bracket kit required for use with Avanti J Series centrifuges, and pump
system required for all applications.
Max. RPM
Max. g
k Factor
Rotor Capacity
20 000
39 900
779
1 750 mL
Interchangeable Cores and Accessories
335130
350641
350601
335141
343348
350585
335142
335134
Continuous-flow Core, Standard
Continuous-flow Core, Large-Pellet
Continuous-flow Core, Small-Pellet
Zonal Core
Zonal Keylock Switch for J2-21
Reorienting Gradient Core and Sample Transfer Assembly
Standard-flow, Rotating Seal Assembly required for Zonaland Continuous-flow Operation
High-flow Seal Assembly Kit: permits processing up to 100
liters/hour in Continuous-flow Core
JCF-Z-Rotor Replacement Parts
335144
812715
815473
824412
854519
366190
870655
870688
335143
Rotating Seal, Carbon-graphite
O-ring for JCF-Z Rotor Plug (Min. order 12)
O-ring for JCF-Z Rotor Lid Stem, 5⁄8” O.D. (Min. order 6)
O-ring for JCF-Z Rotating Seal, 3⁄4” O.D. (Min. order 6)
O-ring for bottom of JCF-Z-Rotor Bowl (Min. order 6)
Bearing, Stainless Steel
O-ring for JCF-Z Rotating Seal, .502 O.D. (Min. order 6)
O-ring for JCF-Z Rotor Lid, 7.188 O.D.
Tool Kit
No. 363843. Bracket Kit, for use with Avanti J-25 Series and Avanti J-30I
centrifuges, and standard Cole-Parmer size-16 tubing (6.4 mm, 1/4-in. O.D.).
For other size tubing, see optional kits below.
No. 366431. Bracket Kit, for use with Avanti J-26 XP Series centrifuges, and
standard Cole-Parmer size-16 tubing (6.4 mm, 1/4-in. O.D.). For other size
tubing, see optional kits below.
No. 363844. Size-14 Tubing Adapter Kit, for use with Cole-Parmer size-14
tubing (4.8 mm, 3/16 in.). Order in addition to one of above Bracket Kits.
No. 363845. Size-15 Tubing Adapter Kit, for use with Cole-Parmer size-15
tubing (9.5 mm, 3/8 in.). Order in addition to one of above Bracket Kits.
Recommended Pumps
The chosen pump must meet the following requirements: (a) has a continuously adjustable flow
rate from 6 to 100 mL/min; (b) has a flow rate controllable to within ±1.5%; (c) has a flow rate
independent of back pressure up to 40 psi; and (d) has pulsation less than 5% of flow rate.
We recommend Cole-Parmer Masterflex L/S Standard Digital Pump E-77921-00 (115 VAC),
or L/S Standard Digital Pump E-77921-07 (230 VAC). These are not available from Beckman
Coulter, but may be ordered direct from Cole-Parmer (625 East Bunker Court, Vernon Hills,
Illinois 60061-1844 USA, Phone 847-549-7600, Fax 847-549-7676, TDD hotline: 800-8337400, E-mail: [email protected]). Contact Cole-Parmer directly to receive current ordering
information for these or comparable pumps.
These pumps include: Easy-Load* pump head model E-07518-12 (accepts L/S 15 and L/S
24 tubing), 10 ft. (3 m) of Tygon† LFL L/S 24 tubing model E-06429-24 (flow range of 28 to
1700 ml/min), and 10 to 600 rpm standard digital drive model E-07523-20 (115 VAC) or
E-07523-27 (230 VAC).
1-62
Small Pellet
Core
Reorienting
Gradient
Core
Large Pellet
Core
Zonal
Core
Standard
Continuous
Flow Core
For use in Avanti J Series centrifuges
(except Avanti J-E).
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JCF-Z (continued)
JCF-Z
Many of the parts on this list are pictured for easy identification.
Supply List
336079
366190
336285
350585
335136
335146
344108
338672
336080
336284
870526
817033
824412
824464
870656
870315
870689
335120
335132
336286
804063
870690
335133
335148
339555
306812
870654
335145
336403
306653
878279
853118
853391
870658
*
Bearing Housing (anodized aluminum)
Bearing (stainless steel)
Bearing Spacer (stainless steel)
Reorienting Gradient Core and Sample Transfer Assembly
Feed Fitting, High-flow (stainless steel)*
Feed Fitting, Standard-flow (stainless steel)*
Fitting Retaining Clamp (modified polyphenylene oxide)
Housing, High-flow Stationary Seal (stainless steel)
Housing, Standard-flow Stationary Seal (stainless steel)
Mount, Rotating Seal (stainless steel)
O-ring, Continuous-flow Lid
O-ring, (Viton13), Continuous-flow Lid
O-ring, Rotating Seal
O-ring, Seal Housing Feed Fitting
O-ring, (Viton13), Transfer Tube Assembly
O-ring, Zonal Lid
Retaining Ring (stainless steel)
Sample Transfer Tube, Standard-flow Seal Assembly
(stainless steel)
Sample Transfer Tube, High-flow Seal Assembly
(stainless steel)
Scavenger Drain Tube (stainless steel)
Screw (stainless steel)
Screw (stainless steel)
Seal Assembly, High-flow
Silicone Vacuum Grease
Solution 555 Rotor Cleaning Concentrate
Spinkote Lubricant
Spring (stainless steel)
Stationary Seal (ceramic)
Stoppers for Tubing Lines (#1, one-hole)
Tygon Tubing
Washer, Flat (nylon)
Washer, High-flow Seal
Washer, Flat (stainless steel)
Washer, Spring (spring steel)
1
344108
870690
853118
Manifold
336080
824464
336286
335146
Standard-flow (SF) Seal Housing
High-flow (HF)
Seal Housing Assembly
335133
870655
868638
344108
335145
870689
335144
824412
335146
824464
870656
Housing
335120 (SF) or
335132 (HF); and
306653
870656
870689
870689
870656
Use Loctite grade “T” primer and #35 retaining compound to bond fitting to seal housing.
853391
870654
853391
336284
870656
336079
Transfer Assembly
(reograd only)
878279
804063
344269
870658
Seal and Transfer Assemblies
336285
344269
Seal Assembly
(all other cores)
Beckman Coulter High Performance Centrifuges
Note: Standard-flow Manifold consists of part numbers 870690, 853118, 336080, 870655, 868638,
and 335145. High-flow Manifold is part of High-flow Seal Housing Assembly, which consists of part
numbers 870690, 853118, 338672, 2 x 335136, and 336286.
1-63
www.beckmancoultercentrifugation.com
JE-5.0
JE-5.0
Elutriation
Elutriator Rotor
For use in Avanti J-26 XP Series and J6-MI centrifuges equipped with
viewport door and strobe assembly.
Axis of
Rotation
1
reb and rmin
rmax
Max. RPM
Max. g
Maximum
Volume
Elutriation
Boundary
5 000
4 700
40 mL
86 mm (2,410 g)
For use in Avanti J-26S centrifuge.*
Centrifugal Force
reb= Elutriation Boundary
No. 356900. JE-5.0 Elutriator Rotor Assembly. Includes bypass chamber
and supplies, and features a quick-release assembly which can be
autoclaved for sterile operation. Order elutriation chamber(s) separately.
(For complete Elutriation System, a number of components are
necessary. See How to Order below.)
Elutriation Chamber
Description
Part No.
Max. Cells
Recovered
per Run
Minimum Cells
Required for
Loading per
Fraction
Max
Elutriated
Buffer
Volume
40-mL
5-mL
Sanderson
356940
356943
356945
1010
1010
1010
107
107
105
1000 mL
1000 mL
1000 mL
How to Order
For a complete Beckman Coulter Elutriation System, the following
components are required:
1. An Elutriator Rotor Assembly
2. An Elutriation Chamber
3. An Elutriation Centrifuge equipped with viewport
door and strobe assembly.
Beckman Coulter Avanti J-26S centrifuges can be ordered as Elutriation
Centrifuges fully equipped with the appropriate door and strobe assembly. See Section 1 for ordering information.
Also necessary but not available from Beckman Coulter is a pump
that meets the following requirements: (a) has continuously adjustable
flow rate from 6 to 100 mL/min; (b) has flow rate controllable to within ±
1.5%; (c) has flow rate independent of back pressure up to 40 psi; and
(d) has pulsation less than 5% of flow rate. Recommended pumps are
listed below.
Recommended Pump (not supplied by Beckman Coulter)
We recommend the Cole-Parmer Masterflex Digital Drive Pump
System. These are not available from Beckman Coulter, but may be
ordered directly from Cole-Parmer (625 East Bunker Court, Vernon
Hills, Illinois 60061-1844 USA, Phone 847-549-7600, Fax 847-5497676, TDD hotline: 800-833-7400, E-mail: [email protected]).
Contact Cole-Parmer directly to receive current ordering information
for these or comparable pumps.
A complete pump consists of one each of the following components:
1. Easy-Load pump head (stainless steel)
2. Tygon L/S 16 tubing standard drive (10 to 600 rpm, 115 VAC or 230
VAC)
Supplies
335148
339555
306812
357520
357580
Silicone Vacuum Grease, 1 oz
Solution 555 Cleaning Concentrate, 1 qt
Spinkote Lubricant (for rotor/shaft interface), 2 oz
Tubing, Tygon 1/8-in. (I.D.) × 1/4-in. (O.D.)
Tubing, Silicone 3/16-in. (I.D.) × 5/16-in. (O.D.)
(for rotating seal-assembly connection)
Tools
927784
016223
927766
029840
001884
817305
Pliers for Retaining Clip (bearing removal)
Wrench, 1/8-in. Hex Driver
Wrench, 5/16-in. Hex T-handle
Wrench, 5/32-in. Hex Driver
Wrench, 5/64-in. Hex Driver
Wrench, 9/64-in. Hex Angle
To Upgrade an Existing Beckman Coulter Centrifuge
For Avanti J-26 XP Series, order the appropriate kit listed below (order
only one kit):
No. 366562. 50/60 Hz. For use with Avanti J-26 XP. Kit includes Strobe
Lamp/Control Assembly and Door Assembly with Viewport.
No. 366563. 50/60 Hz. For use with Avanti J-26 XPI. Kit includes Strobe
Lamp/Control Assembly and Door Assembly with Viewport.
* When properly equipped for elutriation.
1-64
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
JE-5.0 (continued)
Replacement Parts
358291
366922
358182
355161
356014
356940
356941
356943
356944
356945
356946
849660
356917
356907
356935
359399
356930
356921
356942
927768
000148
870671
356912
356911
870655
011519
868638
853984
853696
010426
356910
340148
347549
878681
356915
927286
335213
335197
803993
927569
841717
824905
929279
347526
335145
347543
356913
357459
340145
356920
356919
356914
870669
021623
887438
889303
927730
927731
852685
878475
347978
JE-5.0
Elutriation
927569
356915
Quick-release Seal Assembly 5.0 with counterbalance
Anchor Cable Assembly for Avanti J-26 XP Series centrifuge
853696
853984
Anchor Cable Assembly for J6-MI centrifuge
356914
Ball Bearing, S.C.D.
853984
Cable Retainer
Chamber, Large (40-mL), “A”
356912
Chamber, Large (40-mL), “B”
Chamber, Standard (5-mL), “A”
Chamber, Standard (5-mL), “B”
340145
Chamber, Sanderson (5-mL), “A”
357459
Chamber, Sanderson (5-mL), “B”
347526
Chamber Mounting Nut
Chamber Pad
363328
803993
Chamber Gasket, 40-mL
927731
Chamber Gasket, 4-mL
Chamber Gasket, Sanderson, 4-mL
Chamber Mount
Chamber Tube (solid)
356910
Counterbalance
Fitting, Inlet Line (to seal assembly), 3/16 in. I.D.
010426 (O-ring)
Fuse, Strobe, 1.0 A-TD 250 VAC
Chamber
(Solid)
356921
Glass Plate for Lapping Seal (4 x 5 in.)
Gasket
356919
Housing, Bearing
40-mL, 356907
356920
335145
Housing, Seal
4-mL, 356935
868638
O-ring, Small, Seal Housing
Sanderson, 359399
870655
O-ring, Small, Bearing Shaft
O-ring, Large, Seal Housing
O-ring, Center Tube
964910
O-ring, Retaining, Center Tube
O-ring, Transfer Tube
964942
889303
Plate, Chamber/Seal Support
824905
Pressure Gauge Assembly
929279
Reflector, rpm
Retaining Clip, Bearing Assembly
356917
Retaining Plate, Transfer Tube
Retaining Ring, Internal
Sample Reservoir, 30-mL
Sample Reservoir, 75-mL
Screw, Cap (support plate to seal housing)
Screw, Cap (retaining plate)
Screw, Chamber Mounting
Screw, Housing
Screw, Shoulder (chamber assembly)
Seal, Rotating, Black
Seal, Stationary, White
Syringe Assembly
Shaft (bearing)
Spacer (spring/seal)
Spring (seal assembly)
Tube, Long
Tube, Short
Tube, Transfer
Valve, Stopcock (3-way)
Exploded View of JE-5.0 Parts with Part Numbers
Washer, Flat (bearing housing to support plate)
Washer, Flat (bearing housing to support plate)
Washer, Flat (seal housing)
Washer, Spring (retaining plate)
Washer, Spring (support plate to seal housing)
Washer, Spring (bearing assembly, 3 required)
“Y” Hose Fitting
Flash Tube
Beckman Coulter High Performance Centrifuges
1-65
1
www.beckmancoultercentrifugation.com
Tubes and Bottles
Tubes and Bottles for Every Application
N
2
o single tube design or material will meet all
application requirements. A number of factors
should be considered when a supply of tubes is
ordered: the particular technique to be used, the nature of
the sample and any solvent or gradient media, the desirability
of reusing the tubes, and certain convenience factors. The
properties listed below provide a guide for anyone involved in
the tube selection process.
• Strength and Flexibility, to resist permanent deformation even
when run in fixed angle rotors without tube caps
• Chemical Resistance to a wide range of bases, acids, and solvents
• Transparency to permit a clear view of fractions and bands after
centrifugation
• Thin enough to be sliced or punctured after centrifugation for
fraction collection
• Impermeable to Water to prevent aqueous solutions from
permeating the tube wall and reaching the rotor cavity
• Surface Properties that prevent the adherence of nucleic acids and
proteins
• Temperature Tolerance throughout a wide range of operating
temperatures, without deforming at high temperatures or cracking
when used close to 0°C
• Autoclavable for convenient sterilization and reuse
• Contaminant-free to avoid leaching extraneous materials
into the sample, especially materials visible in the sensitive
240-280 nm range
• Odor-free for pleasant handling
The full line of Beckman Coulter tubes includes a number of
tube materials, each with its own distinct combination of
properties to meet a variety of application requirements.
Available are transparent, translucent, and opaque tubes;
tubes that can be sliced or punctured; tubes that can be
sterilized and reused; and tubes that are resistant to a variety
of chemical compounds.
Beckman Coulter High Performance Centrifuges
2-1
www.beckmancoultercentrifugation.com
Tubes and Bottles
Tube Selection Considerations
Compatibility of Tube Material with Solvents and Sample
2
The chemical compatibility of the tube materials with the gradientforming medium or other solvent is a prime consideration. Neutral
sucrose and salt solutions cause no problem. But alkaline solutions,
such as those frequently used for the separation of single-stranded
forms of DNA, cannot be used in Ultra-Clear tubes or polycarbonate
tubes and bottles. Sometimes DMSO is used in preparation of
sucrose gradients for sedimentation of denatured RNA. Polycarbonateand Ultra-Clear tubes are incompatible with DMSO, so
polypropylene tubes should be used.
The last column of the “Quick Reference Chart to Tube
Materials and Their Properties” on page 2-3 gives some guidelines
to the chemical resistances of the various tube materials. It must
be emphasized, however, that other conditions of centrifugation
(g-force, duration of run, etc.) have considerable effect on how well
a tube material will withstand a particular solvent. Beckman Coulter
publication IN-175, “Chemical Resistances for Beckman Coulter
Centrifugation Products” (found on the Beckman Coulter web site at
www.beckmancoultercentrifugation.com) provides more detailed
information about the chemical resistances of the various tube materials.
The wisest course is to test any questionable combination under
operating conditions before making the actual run.
The type of sample, in some cases, will affect selection of a
specific tube material. DNA, in its denatured or single-stranded form,
will adhere to the surface of some tube materials. Polypropylene would
be the best choice. (Most of this work is done in highly alkaline
media which are incompatible with polycarbonate.)
Lipoprotein separations are most often done in Ultra-Clear
tubes because they are clear and sliceable; these properties simplify
fraction location and recovery by tube slicing. When small
lipoprotein samples are to be recovered by a fractionating device and
clear tubes are desirable, there are alternatives: cellulose propionate,
polycarbonate, and Ultra-Clear tubes.
Hazardous materials, either pathogenic or radioactive, should
be centrifuged with extreme care. All possible precautions must be
taken to avoid leakage of the sample into the rotor cavity during
centrifugation.
To determine the optimum tube material for your specific sample
and gradient medium, refer to the quick reference chart on page 2-4.
Gradient Formation and Fractionation
When choosing a tube for a density gradient run, some thought
should be given to gradient formation and fractionation. If the bands
or zones formed during centrifugation are indistinct, they may
not be visible through a translucent material such as polypropylene.
If optimum band visualization is important, Ultra-Clear tubes or
tubes of polycarbonate or cellulose propionate should be used.
Whenever collection of bands or zones must be done by puncturing
the tube or slicing, a thin, flexible tube wall is required. Ultra-Clear
or polypropylene tubes should be used, depending on the need for
transparency.
2-2
As there are currently no wettable plastic centrifuge tubes
available, gradients should be loaded into plastic tubes from the
bottom up to avoid mixing.
High Temperature Centrifugation
Although modern centrifuges and rotors can operate at temperatures
as high as 45°C, one cannot assume that every tube can be safely run
over 25°C. Stainless steel and glass are the only materials which will
not experience some deformation when subjected to high temperatures
and long centrifugation times. Plastic tubes undergo some degree of
softening at temperatures higher than 25°C. Whether or not this will
cause permanent deformation is not a question of temperature alone.
The centrifugal force field used, the duration of the centrifugation, the
type of rotor, and even the tube angle all have an effect.
It’s obviously impossible to give exact temperature limits for
plastic tubes when so many other variables are involved. The
safest policy is to pretest the tubes under the actual experimental
conditions, but with water, rather than a valuable sample.
Tube Sizes
Tube sizes as indicated in the following charts are nominal sizes, and
may vary somewhat from actual filling capacities. If a thickwall tube
is run uncapped, the maximum filling volume will depend on the
tube angle of the rotor to be used. See appropriate rotor instruction
manuals for maximum filling levels of tubes.
Tube Cleaning, Sterilization, and Reuse
If tubes are to be reused, special care must be taken during cleaning
and sterilization. All tubes can be washed by hand with a mild
detergent such as Solution 555 diluted 5-to-1 or 10-to-1 with water.
This is particularly important for polycarbonate tubes and bottles
which should not be exposed to a detergent with a pH higher than 8.
Tubes and bottles should not be washed in commercial dishwashers
as the detergents and high temperatures are too harsh. Solvents such
as alcohol or acetone react unfavorably with many tube materials. If
an organic solvent must be used in the cleaning procedure, consult
bulletin IN-175 for a table of tube material/solvent compatibilities
(or review the same document on the Beckman Coulter web site at
www.beckmancoultercentrifugation.com).
The method chosen for sterilization has direct bearing on the
number of reuses one can expect from a tube. Tubes and bottles
of polypropylene, polyethylene, and glass can all be autoclaved,
although in general, cold sterilization methods are not as harsh
as autoclaving. Cold sterilization is recommended for both
polycarbonate and Ultra-Clear.
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
Tubes and Bottles
Tube Closures
If maximum reuse is a major consideration, either polypropylene
(preferably thickwall) or polycarbonate tubes and bottles should
be selected, and cold sterilization methods used. If these tubes are
run completely filled in swinging bucket rotors, most of them can
be reused a number of times. Chances of permanent deformation
will be greater whenever the tubes are run in fixed angle rotors,
without caps, and/or partially filled. All of these conditions tend
to stress the centripetal edge of the tube unduly. All tubes that
have been used or autoclaved previously must be individually
examined for signs of deformation or cracking before using
them again.
When other considerations have been resolved, convenience may
be a deciding factor. Without a doubt, the most convenient tube
closure is none at all; none are required for tubes run in swinging
bucket rotors.
For tubes run in fixed angle rotors, alternatives to the standard
tube cap assemblies are available. Bottles have three-piece
cap assemblies which are easier to use than the more complex
tube cap assemblies. Polycarbonate bottles are available for
general-purpose fixed angle rotors, and are used frequently for
differential centrifugation where band recovery is not a problem.
Thickwall tubes can be run in all fixed angle rotors without caps,
provided they are partially filled. (Refer to rotor manuals for
more information on fill volumes.)
When closed tubes are required, Beckman Coulter offers some
innovative and convenient options.
A Quick-Reference Chart to Tube Materials and Their Properties
Property
Thinwall
Polypropylene
Thickwall
Polypropylene
Ultra-Clear
Polycarbonate
Polypropylene
Polyethylene
Cellulose
Propionate
transparent
translucent
transparent
transparent
translucent
transparent/translucent
transparent
Autoclaveable
yes
yes
no
yes**
yes
no
no
Puncturable
yes
no
yes
no
no
yes
no
Sliceable
yes
no*
yes
no
no*
no
no*
Reusable
no
yes
no
yes
yes
yes
no
Acids (dilute
or weak)
S
S
S
M
S
S
S
Optical
Acids (strong)
U
S
U
U
S
S
U
Alcohols
(aliphatic)
U
S
U
U
S
S
U
U
Aldehydes
M
M
S
M
M
S
Bases
S
S
U
U
S
S
U
Esters
U
M
U
U
M
S
M
Hydrocarbons
(aliphatic)
U
M
U
U
S
U
S
Hydrocarbons
(aromatic and
halogenated)
U
U
U
U
M
M
S
Ketones
U
M
U
U
M
M
U
Oxidizing
Agents (strong)
U
U
U
M
M
M
M
Salts
S
S
M
M
S
S
S
S = satisfactory resistance
M = marginal resistanceU = unsatisfactory resistance
* Polypropylene, polycarbonate, and cellulose propionate tubes with diameters of 5 to 13 mm may be sliced using the CentriTube Slicer (PN 34796O) and
appropriate adapter plate.
** Table life will be significantly reduced by autoclaving.
Note: This information has been consolidated from a number of sources and is provided only as a guide to the selection of tube materials. Soak tests at 1 g
(at 20°C) established the data for most of the materials; reactions may vary under the stress of centrifugation, or with extended contact or temperature
variations. To prevent failure and loss of valuable sample, ALWAYS TEST SOLUTIONS UNDER OPERATING CONDITIONS BEFORE USE.
Warning: Do not use flammable substances in or near an operating centrifuge.
Beckman Coulter High Performance Centrifuges
2-3
2
www.beckmancoultercentrifugation.com
Tubes and Bottles
General Filling and Sealing Requirements for Tubes and Bottles
Tube or Bottle
Swinging-Bucket Rotors
Fixed-Angle Rotors
Thinwall tubes
Within 2 to 3 mm of top
Full with cap
Thickwall tubes
At least 1/2 full
1/2 full to max. capless level or full with cap
Quick-Seal tubes
Full and heat-sealed
Full and heat-sealed
Bottles
Min. to max. (see rotor manual)
with screw-on cap or cap
assembly
1/2 full to max. (see rotor manual)
with screw-on cap assembly
Open-top tubes
Within 2 to 3 mm of top
Full with cap
Quick-Seal tubes
Not used
Full and heat-sealed
Thickwall tubes
At least 1/2 full
1/2 full capless level or full with cap or cap assembly
Bottles
At least 1/2 full
Min. to max. (see rotor manual)
with screw-on cap or cap assembly
Stainless Steel
Tubes
Any level
Any level with cap or cap assembly
Polypropylene
Tubes and bottles
At least 1/2 full
1/2 to max. capless level or
full with cap or cap assembly
Polyethylene
Tubes
At least 1/2 full
1/2 to max. capless level or full with cap or cap assembly
Polytetrafluoroethylene Tubes and bottles
(Teflon)
At least 1/2 full
1/2 full to max. capless level or full with cap
Polyphenylsulfone
(PPSU)
At least 1/2 full
N/A
Polypropylene
2
Ultra-Clear
Polycarbonate
2-4
Container
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
Tubes and Bottles
High-Performance and High-Capacity Bottles
Nominal
Capacity
Size
mm
Material
Bottle &
Cap Assy
Bottle with
Screw-On Caps
Bottle
Only
Insert
Only
O-ring
Screw-On Cap
Only
10 mL
16 x 80
PC
N.A.
355672
N.A.
N.A.
N.A.
N.A.
10 mL
16.1 x 81.1
PE
N.A.
364695
N.A.
N.A.
N.A.
N.A.
10 mL
16.1 x 81.1
PTFE
N.A.
364693
N.A.
N.A.
N.A.
N.A.
26.3 mL
25 x 89
PC
355616
N.A.
340382
335258
870385
335259
30 mL
25.3 x 92
PE
363073
N.A.
N.A.
N.A.
N.A.
N.A.
30 mL
25.3 x 92
PC
N.A.
363070
N.A.
N.A.
N.A.
N.A.
40 mL
29 x 104
PC
N.A.
355628
N.A.
N.A.
N.A.
N.A.
50 mL
28.5 x 107
PTFE*
N.A.
363076
N.A.
N.A.
N.A.
N.A.
50 mL
29 x 104
PE
357001
361694
357003
N.A.
358627
961582
356284
50 mL
29 x 104
PC
357000
361693
357002
N.A.
358627
961582
356284
70 mL
38 x 102
PC
355620
N.A.
355655
334545
870384
334547
85 mL
38 x 104
PC
363081
N.A.
N.A.
N.A.
N.A.
N.A.
85 mL
38 x 104
PP
N.A.
364719
N.A.
N.A.
N.A.
N.A.
363082
N.A.
N.A.
N.A.
N.A.
100 mL
38 x 102
PP
355624
355624
355626
N.A.
N.A.
355625
180 mL
55 x 104
PE
N.A.
361245
N.A.
N.A.
N.A.
N.A.
230 mL
62 x 141
PE
356989
N.A.
356988
N.A.
N.A.
344691
230 mL
62 x 141
PC
356987
N.A.
356986
N.A.
N.A.
344691
250 mL
62 x 120
W PC
356013
355673
358275
N.A.
927860
358977
250 mL
62 x 122
W PP
356011
N.A.
358326
N.A.
927860
358977
250 mL
round bottom
62 x 136
PC
N.A.
355673
N.A.
N.A.
N.A.
356261
500 mL
69 x 160
W PC
355605
355664
355649
334419
870411
356260
500 mL
69 x 160
W PP
355607
355665
355650
334419
870411
356260
500 mL
69 x 160
W PC
361690
N.A.
355649
N.A.
927860
360954
500 mL
69 x 160
W PP
361691
N.A.
355650
N.A.
927860
360954
750 mL
96 x 130
PC
N.A.
358299
358297
N.A.
N.A.
344693
750 mL
96 x 130
PP
N.A.
356855
349815
N.A.
N.A.
344693
1000 mL
95 x 191
PC
A98812
N.A.
366751
393898
A98817
B10317
1000 mL
95 x 191
PP
A98813
N.A.
A98814
393898
A98817
B10317
1000 mL
95 x 191
PP
B29896
N.A.
A98814
393898
A98817
B29894
1000 mL
97 x 167
PC
N.A.
355675
341984
N.A.
N.A.
344693
1000 mL
97 x 167
PP
N.A.
355676
341985
N.A.
N.A.
344693
2
PA = Polyallomer PC = Polycarbonate PE = Polyethylene PP = Polypropylene PPSU = Polyphenylsulfone
PTFE = Polytetrafluoroethylene C = Conical W = Wide-Mouth N.A. = Not Available
* With high-speed screw-on caps.
Beckman Coulter High Performance Centrifuges
2-5
www.beckmancoultercentrifugation.com
Tubes and Bottles
Adapters and Sleeves for Non-Beckman Coulter Tubes/Bottles*
2
Rotor
Tube Vol.
mm
Size
# of Places
Number
Part
Rotor
Tube Vol.
Size
mm
# of Places
Part
Number
JA-30.50
5 mL
7 mL
12 x 75
13 x 100
1
1
356970
356976
JA-10
JA-25.50
5 mL
7 mL
12 x 75
13 x 100
1
1
356970
356976
5 mL
7 mL
15 mL
15 mL
50 mL
50 mL
12 x 75
13 x 100
Conical
Round-bottom
Conical
Round-bottom
10
9
5
5
1
1
356967
356973
356960
356994
356965
356996
JA-21
5 mL
12 x 75
1
356972
JA-20.1
5 mL
7 mL
12 x 75
13 x 100
1
1
356971
356977
JS-13.1
5 mL
7 mL
12 x 75
13 x 100
1
1
356970
356976
JA-20
5 mL
7 mL
12 x 75
13 x 100
1
1
356970
356976
JS-7.5
JA-18
5 mL
7 mL
15 mL
50 mL
12 x 75
13 x 100
Conical
Conical
3
3
1
1
356969
356975
356962
356963
5 mL
7 mL
15 mL
50 mL
12 x 75
13 x 100
Conical
Conical
9
8
4
1
356968
356974
356964
356966
JA-17
5 mL
7 mL
12 x 75
13 x 100
1
1
356970
356976
JS-4.2 &
JS-4.2A
3 mL
15 mL
250 mL
Conical
Conical
Conical
26
14
1
339100
339102
349849
JLA 16.250/
JA-14
5 mL
7 mL
15 mL
15 mL
50 mL
50 mL
12 x 75
13 x 100
Conical
Round-bottom
Conical
Round-bottom
9
8
4
5
1
1
356968
356974
356964
356995
356966
356997
JLA-10.500
5 mL
7 mL
15 mL
15 mL
50 mL
50 mL
12 x 75
13 x 100
Conical
Round-bottom
Conical
Round-bottom
10
9
5
5
1
1
356967
356973
356960
356994
356965
356996
Adapters for Glass Tubes in Beckman Coulter Rotors*
Rotor
Volume
Tube
Tube Material
Part Number
Adapter
Rotor
Tube
Volume
Tube Material
Adapter
Part Number
JA-30.50
15 mL
30 mL
Corex, Pyrex
Corex
870329
870331
JS-13.1
30 mL
15 mL
Corex
Corex, Pyrex
870331
870329
JA-25.50
15 mL
30 mL
Corex, Pyrex
Corex
870329
870331
JS-7.5
JA-20.1
15 mL
Pyrex
342643
150 mL
30 mL
15 mL
Corex
Corex
Corex, Pyrex
339362
356997/870331
356995
JA-20/JA-17 15 mL
30 mL
Corex, Pyrex
Corex
870329
870331
JA-18
15 mL
30 mL
Corex, Pyrex
Corex
870329/347539
870331/347539
JA-14
150 mL
30 mL
15 mL
15 mL
Corex
Corex
Corex
Corex, Pyrex Conical
339362
356997/870331
356995
356964
JA-10.500
150 mL
30 mL
15 mL
Corex
362750/339362
Corex
356996/870331
Corex, Pyrex Conical 356960
JA-10
150 mL
30 mL
15 mL
Corex
362750/339362
Corex
356996/870331
Corex, Pyrex Conical 356960
*
JS-4.2 &
JS-4.2A
3 mL
15 mL
30 mL
150 mL
Pyrex Conical
339100
Corex, Pyrex,
339102
Corex & Pyrex Conical
and Conical Graduated
Corex
341977
Corex
339108/
339362
Check with tube manufacturer for maximum allowable g-force.
2-6
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
Tubes and Bottles
Tubes and Bottles Used in High-Performance and High-Capacity Rotors
Nominal
Nominal
Filling
Size
Capacity (mL) (mm)
Inches
Part
No.
Rotors
Quick-Seal Polypropylene Tubes
100.0
38 x 102
1.5 x 4.0
345776
JA-18
345778
JA-18
Quick-Seal Ultra-Clear Tubes
100.0
38 x 102
1.5 x 4.0
Quick-Seal Bell-Top Tubes, Polypropylene
4.3
16 x 38
0.65 x 2.5
356562
JS-24.15
6.3
16 x 45
0.65 x 1.8
345830
JS-24.15
8.0
16 x 58
0.65 x 2.25
344621
JS-24.15
8.5
25 x 38
0.65 x 2.5
358652 (konical)
JS-24.38
10.0
16 x 67
0.65 x 2.5
344622
JS-24.15
15.0
25 x 38
1 x 1.5
343664
JS-24.38
23.0
25 x 76
1 x 1.5
358654 (konical)
JS-24.38
27.0
25 x 64
1 x 2.5
343665
JS-24.38
33.0
25 x 83
1 x 3.25
344623
JS-24.38
2
Open-Top Tubes, Polypropylene
15.0
16 x 96
0.65 x 3.85
361707
JS-24.15
25.0
25 x 76
1.0 x 3.0
358125 (konical)
JS-24.38
30.0
25 x 89
1.0 x 3.5
358126 (konical)
JS-24.38
38.5
25 x 89
1.0 x 3.5
326823
JS-24.38
Open-Top Tubes, Polypropylene, Thickwall
4.0
13 x 64
0.5 x 2.5
355644
JA-25.15
10.0
16 x 76
0.65 x 3.0
355640
JA-25.15, JA-21, JA-20.1, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
12.5
16 x 95
0.625 x 3.75
32.0
25 x 89
1.0 x 3.5
361708 (konical)
JS-24.15
355642
JS-24.38
Open-Top Tubes, Polycarbonate
15.0
18 x 100
0.725 x 4.0
342080
JA-25.15, JA-20.1, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
50.0
29 x 104
1.125 x 4.0
363647
JA-30.50, JA-25.50, JA-20, JA-18, JA-17, JA-14, JLA-10.500, JA-10,
JLA-9.1000, JLA-8.1000, JS-7.5, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0,
JS-3.0
1000.0
95 x 191
366751
JLA-9.1000, JLA-8.1000
3.8 x 7.65
Open-Top Tubes, Polycarbonate, Thickwall
4.0
13 x 64
0.5 x 2.5
355645
JA-25.15
10.0
16 x 76
0.65 x 3.0
355630
JA-25.15, JA-21, JA-20.1, JS-13.1, JS-5.2, JS-4.3, JS-4.2, JS-4.2A,
JS-4.0, JS-3.0
32.0
25 x 89
1.0 x 3.5
355631
JS-24.38
342081
JA-25.15, JA-20.1, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
Open-Top Tubes, Polyethylene, Thickwall
15.0
18 x 100
0.725 x 4.0
Beckman Coulter High Performance Centrifuges
2-7
www.beckmancoultercentrifugation.com
Tubes and Bottles
Nominal
Nominal
Filling
Size
Capacity (mL) (mm)
Inches
Part
No.
Rotors
Open-Top Tubes, Polypropylene, Thickwall
2
4.0
13 x 64
0.5 x 2.5
355645
JA-25.15
15.0
18 x 100
0.725 x 4.0
342082
JA-25.15, JA-20.1, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
50.0
29 x 104
1.125 x 4.0
357007
JA-30.50 Ti, JA-25.50, JA-20, JA-18, JA-17, JLA-16.250, JA-14,
JLA-10.500, JA-10, JLA-9.1000, JLA-8.1000, JS-7.5, JS-5.2, JS-4.3,
JS-4.2, JS-4.2A, JS-4.0, JS-3.0
1000.0
95 x 191
A98814
JLA-9.1000, JLA-8.1000
3.8 x 7.65
Open-Top Tubes, Ultra-Clear
15.0
16 x 96
0.65 x 3.8
361706
JS-24.15
38.5
25 x 89
1.0 x 3.5
344058
JS-24.38
357448 - Natural
JA-30.50 Ti, JA-25.50, JA-20, JA-18.1, JA-18, JS-13.1, JS-5.2, JS-4.3,
JS-4.2, JS-4.2A, JS-4.0, JS-3.0
363664
JA-30.50 Ti, JA-25.50, JLA-16.250, JA-14, JLA-10.500, JA-10,
JLA-9.1000, JLA-8.1000, JS-13.1, JS-7.5, JS-5.2, JS-4.3, JS-4.2,
JS-4.2A, JS-4.0, JS-3.0
Tubes with Snap-On Caps, Polypropylene
1.5
11 x 38
0.4 x 1.5
Tubes with Snap-On Caps, Polycarbonate
50.0
29 x 104
1.125 x 4.0
Tubes with Snap-On Caps, Polypropylene
1.5
11 x 38
0.4 x 1.5
356090 - Natural
JA-30.50 Ti, JA-25.50, JA-20, JA-18, JA-18.1, JS-13.1, JS-5.2, JS-4.2,
JS-4.3, JS-4.2A, JS-4.0, JS-3.0
1.5
11 x 38
0.4 x 1.5
356091 - Blue
JA-20, JA-18, JA-18.1, JS-13.1,JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0,
JS-3.0
1.5
11 x 38
0.4 x 1.5
356092 - Green
JA-20, JA-18, JA-18.1, JS-13.1,JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0,
JS-3.0
1.5
11 x 38
0.4 x 1.5
356093 - Yellow
JA-20, JA-18, JA-18.1, JS-13.1,JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0,
JS-3.0
1.5
11 x 38
0.4 x 1.5
356094 - Orange
JA-20, JA-18, JA-18.1, JS-13.1,JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0,
JS-3.0
1.5
11 x 38
0.4 x 1.5
343169- Natural
(cap separate)
JA-18.1, JA-18, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
1.125 x 4.0
357005 - Natural
(cap separate)
JA-30.50 Ti, JA-25.50, JA-20, JA-17, JLA-16.250, JA-14, JLA-10.500,
JA-10, JLA-9.10000, JLA-8.1000, JS-13.1, JS-7.5, JS-5.2, JS-4.3,
JS-4.2, JS-4.2A, JS-4.0, JS-3.0
340196 - Natural
JA-20, JA-18.1, JA-18, JS-13.1, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0,
JS-3.0
50.0
29 x 103
Tubes with Snap-On Caps, Polyethylene
1.8
11 x 39
0.4 x 1.5
Bio-Vial Tubes
4.0
14 x 55
.5625 x 2.25
566353 - Polypropylene JLA-16.250, JA-14, JLA-10.500, JA-10, JLA-9.1000, JLA-8.1000, JS-7.5,
JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
Bottles Only
70.0
38 x 102
1.5 x 4.0
355655 - Polycarbonate JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
100.0
38 x 102
1.5 x 4
355626
500.0
69 x 160
2.75 x 6.5
355649 - Polycarbonate JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
500.0
69 x 160
2.75 x 6.5
355650
1000.0
95 x 191
3.75 x 7.5
366751 - Polycarbonate JLA-9.1000, JLA-8.1000
1000.0
95 x 191
3.75 x 7.5
A98814 - PolypropyleneJLA-9.1000, JLA-8.1000
2-8
JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
Tubes and Bottles
Nominal
Nominal
Filling
Size
Capacity (mL) (mm)
Inches
Part
No.
Rotors
Bottles with Cap Assemblies, Polypropylene
50.0
29 x 104
1.25 x 4.0
357001
JA-30.50 Ti, JA-20, JA-17, JLA-16.250, JA-14, JLA-10.500, JA-10,
JLA-9.1000, JLA-8.1000, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
50.0
29 x 104
1.25 x 4.0
361694
JA-25.50, JA-17, JA-14, JS-5.2, JS-4.3, JS4.2, JS-4.2A, JS-4.0, JS-3.0
Bottles with Cap Assemblies, Polycarbonate
50.0
29 x 104
1.25 x 4.25
361693
JA-25.50, JA-17, JS-4.3
50.0
29 x 104
1.25 x 4.25
357000
JA-30.50 Ti, JA-20, JA-17, JLA-16.250, JA-14, JLA-10.500,
JA-10, JLA-9.1000, JLA-8.1000, JS-7.5, JS-5.2, JS-4.3, JS-4.2,
JS-4.2A, JS-4.0, JS-3.0
70.0
38 x 102
1.5 x 4.0
355620
JA-18, JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
500.0
62 x 166
2.75 x 6.5
361690
JLA-10.500
250.0
62 x 122
358275 (wide mouth)
JS-5.2, JS-4.2, JS-4.3, JS-4.2A, JS-4.0, JS-3.0
500.0
69 x 160
355605 (wide mouth)
JA-10, JLA-9.1000, JLA-8.1000, JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
1000.0
95 x 191
A98812
JLA-9.1000, JLA-8.1000
2.5 x 4.75
2.75 x 6.5
3.8 x 7.65
2
Bottles with Cap Assemblies, Polypropylene
10.4
16 x 76
0.6 x 3.0
355603
JS-7.5
250.0
62 x 120
2.5 x 4.75
358326 (wide mouth)
JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
500.0
69 x 160
2.75 x 6.5
355607 (wide mouth)
JA-10, JLA-9.1000, JLA-8.1000, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0,
JS-3.0
2.75 x 6.5
500.0
69 x 160
361691
JLA-10.500
1000.0
95 x 191
3.8 x 7.65
A98813
JLA-9.1000, JLA-8.1000
1000.0
95 x 191
3.8 x 7.65
B29896
JLA-9.1000, JLA-8.1000
Bottles with Screw-On Caps, Polypropylene
50.0
29 x 104
1.25 x 4.0
357003
JA-30.50 Ti, JA-25.50, JA-20, JA-18, JA-17, JLA-16.250, JA-14,
JLA-10.500, JA-10, JLA-9.1000, JLA-8.1000, JS-13.1, JS-7.5, JS-5.2,
JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
Bottles with Screw-On Caps, Polycarbonate
10.0
16 x 80
0.6 x 3.2
355672
JA-30.50 Ti, JA-25.50, JA-25.15, JA-21, JA-20.1, JA-20, JA-17, JS-13.1,
JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
50.0
29 x 104
1.25 x 4.0
357002
JA-30.50 Ti, JA-25.50, JA-20, JA-18, JA-17, JLA-16.250, JA-14, JLA10.500, JA-10, JS-13.1, JS-7.5, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0,
JS-3.0
230.0
62 x 141
2.5 x 5.25
356987 (conical)
JA-14, JS-7.5, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
250.0
62 x 120
2.5 x 4.75
356013 (wide mouth)
JLA-16.250, JA-14, JLA-10.500, JA-10, JLA-9.1000, JLA-8.1000,
JS-7.5, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
250.0
62 x 136
2.5 x 5.5
355673
JS-7.5, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
500.0
69 x 160
2.75 x 6.5
355664
JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
750.0
96 x 130
3.75 x 5.25
358299
JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
1000.0
97 x 167
3.75 x 6.75
355675
JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
Bottles with Screw-On Caps, Polypropylene
100.0
38 x 102
1.5 x 4.0
355624
JA-18, JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
230.0
62 x 141
2.5 x 5.5
356989 (conical)
JA-14, JS-7.5, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
250.0
62 x 120
2.5 x 4.75
356011 (wide mouth)
JLA-16.250, JA-14, JLA-10.500, JA-10, JLA-9.1000, JLA-8.1000,
JS-7.5, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
500.0
69 x 159
2.75 x 6.5
355665
JA-10, JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
750.0
96 x 130
3.75 x 5.25
356855
JS-5.2, JS-4.3, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
1000.0
97 x 167
4.0 x 6.75
355676
JS-5.2, JS-4.2, JS-4.2A, JS-4.0, JS-3.0
BottleswithScrew-OnCaps,Polytetrafluoroethylene(PTFE)
50.0
28.5 x 107
1.25 x 4.25
363076
Beckman Coulter High Performance Centrifuges
JS-4.2
2-9
www.beckmancoultercentrifugation.com
HarvestLine System Liners
Unique design enhances productivity
2
HarvestLine disposable bottle liners provide a significant
improvement in the centrifugation of biological material.
HarvestLine System Liners save time in the handling and
retrieval of sample. Used in Avanti Series centrifuges, these
unique liners provide a convenient method of loading,
recovering, and storing samples.
HarvestLine liners can be sterilized (gamma radiation),
placed in a freezer (-70°C) for prolonged storage, and are
compatible with commonly used chemicals and solvents.
They facilitate the harvesting of:
• Mammalian and insect culture separations
• Bacterial, yeast, and tissue homogenate isolations
• Virus harvesting for vaccine production—viral vector
gene therapy and monoclonal antibody production.
HarvestLine System for JLA-8.1000 and JLA-9.000 Rotors
JLA-8.1000 and JLA-9.1000 Fixed Angle Rotor with Liners
The 6 x 1 liter JLA-8.1000 rotor system with liners is ideal for
harvesting bacterial and yeast cell cultures from fermenters,
clearing cell debris from homogenates, or purifying largevolume viruses and polysaccharides for vaccine production.
The 4 x 1 liter JLA-9.1000 rotor provides up to 16 800 x g for
high throughput.
JS-5.0 Swinging Bucket Rotor with Liners
The 4 x 2.25 liter JS-5.0 rotor with liners is ideal for greater
sample throughput. Use this system to separate bacterial,
yeast, and tissue homogenates. At 7 480 x g, the rotor harvests
E. coli at a clearing rate of 99% in less than 10 minutes
(throughput of better than 36 liters per hour).
HarvestLine System for JS-5.0 Rotor
Part Numbers
369264
369256
368735
HarvestLine System Liner Kit
Includes369256one-literbottleliners(qty120),368740filling
rack, 977472 funnel, and 369267 instructional CD.
HarvestLine System Liners
Fits JLA-8.1000 or JLA-9.1000 one-liter bottles (qty. 120).
HarvestLine System Liners
Fits JS-5.0 J-Wide Cups (qty. 50).
Specifications
Maximum Speed
in the JS-5.0 rotor
in the JLA-8.1000 rotor
in the JLA-9.1000 rotor
5 000 rpm
8 000 rpm
9 000 rpm
Maximum g-force
in the JS-5.0 rotor
in the JLA-8.1000 rotor
in the JLA-9.1000 rotor
7 480 × g
15 970 × g
16 800 × g
Run Time
Up to 20 min. instrument set time
Temperature Range
4° to 10°C
Acceleration/Deceleration
Up to instrument maximum
settings
2-10
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
Tools and Supplies
Tools and Supplies
Rotor Cleaning Kit
339558 Rotor Cleaning Kit. Contains two 946-mL bottles of
Solution 555 Rotor Cleaning Concentrate, 339379
Rotor Cleaning Brush, and 339380 Rotor Cleaning Brush
Replacement Parts/Supplies
339555 Solution 555 Rotor Cleaning Concentrate
(min. order two Bottles)
339379 Rotor Cleaning Brush, 5⁄8-in. (16 mm) and 1-in. (25.4 mm),
for Rotor Cavity diameters from 7⁄16-in. (11 mm) to 1-in.
(25.4 mm) (min. order three Brushes)
339380 Rotor Cleaning Brush, 11⁄4-in. (32 mm) and 11⁄2-in. (38
mm), for Rotor Cavity diameters from 1-in. (25.4 mm) to
11⁄2-in. (38 mm) (min. order three Brushes)
Beckman Coulter High Performance Centrifuges
2-11
2
www.beckmancoultercentrifugation.com
Reference
Guide to Centrifuge Selection
Centrifugation is a basic separation technique that is utilized at multiple
stages in the study of sample components. Flexible rotor and adapter
systems for each Beckman Coulter centrifuge allow them to be used
across multiple application areas. To help you select the most appropriate
centrifuge for your work, the following charts provide brief descriptions
of the kinds of separations typically achieved using various centrifuges.
These charts list frequent separation requirements for each sample type,
and identify the centrifuges that are typically used to meet those requirements.
In addition to the separation and isolation of sample particles, centrifugation
is increasingly being used as an analytical technique for the study of
macromolecular interactions and the determination of molecular weights.
Instruments for these applications are also listed below.
Quick-Reference Guide to Centrifuge Selection
Proteins
Ammonium sulfate precipitates
Centrifugal filtration
Avanti J
3
Centrifuges Typically Used
•
•
Avanti J-HC
Specific Application
J6-MI
Materials to Be Isolated
•
•
Subcellular Fractions
Chromatin/Nucleosomes
Sucrose gradient isolation
Microsomes
Pelleting
Sucrose gradient isolation
Microsomal membrane fractionation
Mitochondria
Pelleting
Sucrose gradient isolation
Nuclei
Pelleting
Membranes
Pelleting
Sucrose/Percoll gradient fractionation
Binding studies
Ribosomes/Polysomes
Pelleting
Size fractionation in sucrose gradients
Cytosol
Clarification
3-2
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
Reference
Quick-Reference Guide to Centrifuge Selection (continued)
•
•
•
•
•
Preparative Centrifugation
Lysates/Homogenates
Nucleic Acids
DNA
RNA
Cells
Viruses
Blood
Clearing debris and large particles
•
Alcohol precipitation
Phenol/CHC13 extraction
Size fractionation in sucrose gradients
Minipreps in 96-well plates
Spin columns
•
•
•
•
Plasma preparation
Blood-cell products
Beckman Coulter High Performance Centrifuges
•
•
Phenol/CHC13 extraction
Alcohol precipitation
Lithium precipitation
Isolation of mononuclear cells on
Ficoll-Hypaque
Pelleting bacteria
Pelleting mammalian cells
Elutriation of viable cells
Other density gradient separations
Pelleting
PEG precipitates
Density gradient isolations
Avanti J-HC
Centrifuges Typically Used
J6-MI
Specific Application
Avanti J
Materials to Be Isolated
3
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
3-3
www.beckmancoultercentrifugation.com
Reference
Useful Formulas
k Factor
To determine k factor
k=
To determine pelleting time (t)
t=
To relate pelleting time between rotors
ln (rmax/rmin)
ω2
k
s
10-13
3600
OR
k=
2.53 × 105 ln (rmax/rmin)
(RPM/1000)2
where s = sedimentation coefficient in Svedbergs
k1
k
= 2
t1
t2
To adjust k factor for runs less than maximum
rotor speed
3
×
kadj = k
rated speed of rotor
( maximum
)
actual run speed
2
To relate relative centrifugal force (RCF) to speed (RPM):
RCFmax = 1.12 rmax
( RPM
) OR RPM = 10
1000
2
3
RCF
1.12rmax
To relate the sedimentation coefficient (s) to rotational speed:
s=
1
dr
×
ω2r
dt
Svedberg unit (S) equivalent:
S = 10-13 seconds
Reduced run speed for dense solutions:
reduced run speed = max rated speed of rotor ×
A
B
where A = max. permissible density of rotor tube contents, and
B = actual density of the tubes to be centrifuged
3-4
Beckman Coulter High Performance Centrifuges
www.beckmancoultercentrifugation.com
Support Services
Field Service Support
Most of us appreciate the need for careful design, handling,
and maintenance of certain kinds of laboratory equipment—
the optics of a spectrophotometer, for example. It may not
be obvious, however, that anything as substantial looking as
a centrifuge rotor must be properly maintained during use in
order for it to fulfill its life expectancy.
If you consider that, during rotation, a High-Performance
Centrifuge Rotor may experience more than 110,000 times
the force of gravity, it becomes apparent that seemingly
minor flaws will assume much greater significance at these
g–forces. In effect, one gram will “weigh” 110 kilograms,
and a tiny flaw in a critical part may generate stresses greater
than the rotor was designed to withstand.
We at Beckman Coulter maintain stringent quality
standards and rigorously test every rotor design. Specific
instructions for care and maintenance are included in the
rotor manual that accompanies each rotor. And our Field
Rotor Inspection Program is available at no charge to all
users of Beckman Coulter High-Performance Centrifuges
and Ultracentrifuges.
4
Field Rotor Inspection Program. What Is It?
Our Field Rotor Inspection Program (FRIP) has two
purposes: to prevent premature rotor failures by detection of
stress corrosion, metal fatigue, wear or damage to anodized
coatings; and to instruct laboratory personnel in the proper
care of rotors.
Contact your local Beckman Coulter Service Office
for details on the Field Rotor Inspection Program. Using
nondestructive methods (fiber optics borescopy and dyepenetrant analysis), they may find signs of corrosion or other
damage. If so, they will recommend repair or replacement,
and a potentially costly failure may be prevented.
To give a fuller understanding of rotors, a comprehensive
slide presentation is also offered. Centrifuge users are
informed about maintenance procedures, rotor damage, and
its significance. The stress corrosion of aluminum rotors, in
particular, can be greatly reduced by good laboratory practice.
For more information about the Field Rotor Inspection
Program (FRIP), please go to:
http://www.beckmancoulter.com/FRIP.
Sometimes rotor corrosion is so bad it can be seen by the
naked eye, while at other times it is not so apparent. If you
have doubt about the condition of a single Beckman Coulter
4-2
3-6
rotor, you may return it to the factory where it will be
inspected free of charge by our metallurgists. The rotor will be
examined by a variety of nondestructive methods, including
ultrasound which is capable of detecting internal flaws. Before
shipping the rotor, you must contact the nearest Beckman
Coulter Sales and Service office for specific instructions. A
written statement must accompany each rotor, indicating that
it is safe to handle, (i.e. free of any pathogenic or radioactive
contamination).
Rotor Safety Seminars
To help you get the most from your Beckman Coulter rotors,
Rotor Safety Seminars are frequently presented by our Field
Service engineers. Seminars are designed to remind centrifuge
users about the importance of proper rotor care and maintenance.
When you attend a Beckman Coulter Rotor Safety
Seminar, you will have an opportunity to ask questions that
are specific to your particular situation, and you will learn:
•
What causes corrosion in a rotor, where it starts, what it
does to the rotor, and how to avoid it
•
The proper way to clean and store rotors
•
How to get longer life from your rotor
On-Site Rotor Inspection and Maintenance
Specially trained Beckman Coulter Field Service engineers
are available at your request for on-site inspection and minor
rotor maintenance. Rotors that are free of biological and
radiological contamination can be brought to these sessions
for immediate inspection so you can continue operating your
centrifuge with confidence in the condition of your rotors.
A Comprehensive Approach to Centrifuge System Care
Throughout the world, Beckman Coulter Field Service
engineers are on hand to provide the support you need. So
you can operate your centrifuges worry-free, a number of
programs are in place that cover preventive maintenance of
your system, including parts, labor, drive systems, and rotors.
It all adds up to a comprehensive approach to maximizing
your system investment.
Contact your local Beckman Coulter Sales and Service
office to find out the specifics about the program available in
your area.
Beckman Coulter High Performance Centrifuges
.fr
lter
ou
anc
ckm
be
ww.
w
Beckman Coulter France S.A.S.
Paris-Nord 2
22, Av. des Nations
B.P. 54359 – Villepinte
95942 Roissy CDG Cedex
www.beckmancoulter.fr
[email protected]

6. 部品の材質 Materials of Parts 7. アダプタの名称 Name of Adaptors

Download Spec Sheet - Gyro-Trac

The Construction Slideshow

Secure Airway Clamp - SA Medical Research Council

Power Delivery

MEG-2X D - Robert Q. Riley Enterprises

2013 Pricing Guaranteed to August 31, 2014. isoSPEC® Pump

PQR-Low Integral Finned SS Tube for MSR

8T20RB - wvs

Document 2852783