Lipid nanoparticle delivery of RNA for loss-of-function and gain-offunction studies in primary neurons in vitro and in vivo. David Zwaenepoel1, A. S. Ansari1,2, A. Thomas1, C. Walsh1, T. Leaver1, A. Wild1,2, K. Ou1,2, J. Taylor1, E. Ramsay1,2 1Precision NanoSystems, Inc., Vancouver, Canada. Summary 2University of British Columbia, Vancouver, Canada in vitro SUB9KITS™ RNA-Lipid Nanoparticles: Exploit low-density lipoprotein (LDL) receptormediated uptake to transfect primary neurons with high efficiency (> 95%) SUB9 SUB9 KITS™ siRNAsiRNA-Lipid Nanoparticles Mediate >90% 90% Target Gene Knockdown in Primary Rat Cortical Neuron Cultures. Cultures. A high efficiency, low toxicity transfection modality loss-of-function and gain-of-function studies in primary neurons in vitro and in vivo SUB9 siRNA-Lipid Nanoparticles Maintains >70% SUB9 KITS™ siRNA 70% Target Gene Knockdown in Primary Rat Cortical Neuron Cultures for 21 Days. Days. Mediate prolonged (>21 days), dose-dependent target gene knockdown in primary neurons Enable simultaneous knockdown of multiple gene targets in primary neurons Manufacture & Mechanism-of-Action Pten Expression (% Control) Promote mRNA-mediated protein expression in primary neurons Primary E18 rat cortical neurons (DIV 13; mixed culture) were treated with SUB9KITS™ siRNALipid Nanoparticles (10 ng/mL & 100 ng/mL siRNA targeted against Pten gene, or 100 ng/mL non-targeted siRNA control). 72 h posttreatment, Pten mRNA (RT-qPCR) and PTEN protein (Western Blot) were quantified. Cell viability, as determined by lactose dehydrogenase assays, indicated no difference between treated and control cells (data not shown). SUB9 SUB9 KITS™ siRNAsiRNA-Lipid Nanoparticles Mediate Signi fica nt Reduction in Target Protein Knockdown up to 1 mm from the Injection Site Following Direct Injection into Rat Somatosensory Cortex. Cortex. Primary E18 rat cortical neurons (DIV 13; mixed culture) were treated with a single administration of SUB9KITS™ siRNA-Lipid Nanoparticles (100 ng/mL siRNA targeted against Hprt gene, or 100 ng/mL non-targeted siRNA control). Hprt mRNA (RT-qPCR) was quantified of Days 1, 3, 6, 10, 14 and 21 posttreatment. SUB9 SUB9 KITS™ RNARNA-Lipid Nanoparticles are Manufactured Using Microfluidics in vivo Lipid mix Sprague-Dawley rats received a single injection of SUB9KITS™ siRNA nanoparticles (500 nl at 5 mg/mL siRNA for 10 minutes) directly into the somatosensory cortex. Cortical slices were prepared 5 days post-injection, and were analyzed by Western Blot. The results showed a significant reduction in target PTEN protein (> 80%). Conclusion SUB9 SUB9 KITS™ siRNAsiRNA-Lipid Nanoparticles Mediate Simultaneous Multiple Target Gene Knockdown in Primary Rat Cortical Neuron Cultures. Cultures. RNA 150 SUB9KITS™ RNA-Lipid Nanoparticles are manufactured using customized microfluidic mixing devices. Microfluidic mixing mediates production of welldefined neutral RNA-Lipid Nanoparticles that posess a “solid-core” morphology. in vitro Paracrine Factors Primary E18 rat cortical neurons (DIV 13; mixed culture) were treated with SUB9KITS™ Pten siRNA-Lipid Nanoparticles (100 ng/mL siRNA) alone, or SUB9KITS™ Hprt siRNA-Lipid Nanoparticles (100 ng/mL siRNA) alone, or a combination of both nanoparticle preparations. A non-targeted SUB9KITS™ siRNA-Lipid Nanoparticles (100 ng/mL siRNA) was used as a control. Hprt and Pten mRNA llevels were quantified 72 h post-treatment (RT-qPCR). Hprt Pten 100 For more information, go to www.sub9kits.com 50 Acknowledgements and Support 0 Control in vivo SUB9KITS™ RNA-Lipid Nanoparticles are delivered to the cytoplasm by mimicking low-density-lipoproteins (LDL) and co-opting the paracrine factor Apolipoprotein E4 (ApoE4) to mediate cell uptake via the LDL-receptor. Simply adding the nanoparticles to ApoE-containing primary neuron culture results in >95% uptake within 4h (data not shown). SUB9KITS.com SUB KITS.com SUB9KITS™ RNA-Lipid Nanoparticles represent a new, highly effective modality of transfection reagent for loss-offunction and gain-of-function studies in primary neurons in vitro and in vivo. siNT siHprt siPten siHprt siPten SUB9 SUB9 KITS™ mRNAmRNA-Lipid Nanoparticles Mediate Effective Transfection and Expression of GFP in Primary Rat Cortical Neuron Cultures. Cultures. Precision NanoSystems acknowledges the support of the research groups headed by Profs. Brian MacVicar and Yu Tian Wang, University of British Columbia. Primary E18 rat cortical neurons (DIV 13; mixed culture) were treated with fluorescently-labelled SUB9KITS™ GFP mRNA-Lipid Nanoparticles (500 ng/mL GFP mRNA). Confocal images were taken 72h post-treatment to confirm nanoparticle uptake (red, left-hand panel). The right-hand panel shows an overlay of uptake and GFP expression. Flow cytometry analysis indicated particle uptake and subsequent GFP expression in > 90% of primary neurons (data not shown). 1.888.618.0031 · info@precisioninfo@precision -n an o.com