OIE Reference Laboratory Reports Activities

OIE Reference Laboratory Reports Activities
Activities in 2013
This report has been submitted : 2014-02-24 10:54:43
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Rabies
Address of laboratory:
Anses – Laboratoire de la rage et de la faune sauvage de Nancy
Domaine de Pixérécourt Bâtiment H Technopôle agricole et
vétérinaire CS 40009 F-54220 MALZEVILLE FRANCE
Tel.:
+33 (0)3 83 29 89 50
Fax:
+33 (0)3 83 29 89 58
e-mail address:
[email protected]
website:
www.anses.fr; http://www.ansespro.fr/eurl-rabies/index.htm
Name (including Title) of Head of
Laboratory (Responsible
Official):
Elodie Monchatre-Leroy, Directrice
Name (including Title and
Position) of OIE Reference
Expert:
Florence Cliquet
Which of the following defines
your laboratory? Check all that
apply:
Governmental
OIE Reference Laboratory Reports Activities, 2013
1
Rabies - Florence Cliquet - france
ToR: To use, promote and disseminate diagnostic methods validated according to
OIE Standards
1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease
diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff
training)
Yes
Diagnostic Test
Indicated in
OIE Manual
(Yes/No)
Indirect diagnostic tests
Total number of test performed last
year
Nationally
Internationally
Séroneutralisation virale, test FAVN
Oui
1964
1739
Test d'efficacité des vaccins vétérinaires
inactivés
Oui
31
29
Test d'efficacité des vaccins humains inactivés
Non
0
0
RT-PCR temps réel
Oui
358
3287
Titrage de virus rabique
Non
37
96
Nationally
Internationally
Direct diagnostic tests
Diagnostic par immunofluorescence directe
Oui
475
9
Diagnostic par inoculation à une culture
cellulaire de neuroblastomes murins
Oui
158
8
Diagnostic par inoculation à la souris
Oui
0
1
recherche de l'ARN viral
Oui
278
52
Typage de souches
Oui
141
47
ToR: To develop reference material in accordance with OIE requirements, and
implement and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and
any other reagents used in the diagnosis and control of the designated pathogens
or disease.
2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE?
2
OIE Reference Laboratory Reports Activities, 2013
Rabies - Florence Cliquet - france
No
3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents
to OIE Member Countries?
Yes
Type of
reagent
available
Related diagnostic
test
Produced/
provide
Amount
supplied
nationally
(ml, mg)
Amount
supplied
internationally
(ml, mg)
No. of
recipient
OIE
Member
Countries
Region of
recipients
Africa
Sérum
naïf de
référence
Séroneutralisation
virale, ELISA
Produit
25 ml
235 ml
6
Americas
Asia and
Pacific
Europe
Middle
East
Africa
Virus
standard
CVS
Séroneutralisation
virale
Conservé
0
2,5 ml
3
Americas
Asia and
Pacific
Europe
Middle
East
4. Did your laboratory produce vaccines?
No
5. Did your laboratory supply vaccines to OIE Member Countries?
No
ToR: To develop, standardise and validate, according to OIE Standards, new
procedures for diagnosis and control of the designated pathogens or diseases
6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated
pathogen or disease?
No
7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?
No
OIE Reference Laboratory Reports Activities, 2013
3
Rabies - Florence Cliquet - france
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and
technical advice on disease control measures to OIE Member Countries
8. Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
No. samples received
for provision of
diagnostic support
No. samples received for
provision of confirmatory
diagnoses
Name of OIE Member Country
seeking assistance
Date (month)
MALTA
15 mars
1
LUXEMBOURG
14 mai
1
LUXEMBOURG
7 juin
1
THE NETHERLANDS
27 novembre
4
THE NETHERLANDS
27 novembre
4
9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?
Yes
4
Name of the OIE Member
Country receiving a
technical consultancy
Purpose
How the advice
was provided
GREECE
Appui pour la mise en oeuvre de la vaccination orale de
la faune sauvage à l'automne 2013
Sur place et à
distance
LATVIA
Appui pour la mise en place d'une station expérimentale
animale
Sur place
LATVIA
Appui pour la mise en place de la surveillance active de
la rage des chauves-souris
Sur place
TUNISIA
Surveillance de la rage, outils pour l'étude de la
population canine et des mesures de contrôle de la rage
canine
Sur place
MEXICO
Cahier des charges techniques pour accéder à la
nomination en tant que Laboratoire de Référence OIE
Réunion
BOSNIA AND
HERZEGOVINA
Conseils sur la stratégie de surveillance et de contrôle
de la rage
Sur place
BOSNIA AND
HERZEGOVINA
Participation à l'évaluation des besoins en équipements
des laboratoires
Sur place
OIE Reference Laboratory Reports Activities, 2013
Rabies - Florence Cliquet - france
ToR: To carry out and/or coordinate scientific and technical studies in collaboration
with other laboratories, centres or organisations
10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries
other than the own?
Yes
Title of the study
FP7 ICONZ (Integrated
Control of Neglected
Zoonoses in Africa)
FP7 Predemics
(Preparedness,
Prediction and
Prevention of Emerging
Zoonotic Viruses with
Pandemic Potential
using Multidisciplinary
Approaches)
Duration
Purpose of the study
Partners
(Institutions)
5 ans (20092014)
Participer à des études épidémiologiques
au Mali et au Maroc : des zones pilotes ont
été sélectionnées et des études des
populations canines sont menées avec
prises de sang des chiens. Des
questionnaires auprès des propriétaires
permettront de définir le rôle des chiens
dans les zones sélectionnées. L’objectif est
ainsi de pouvoir définir dans ces pays une
stratégie adaptée de contrôle de la rage
animale.
22 institutions,
17 pays
représentés
5 ans (20112016)
Mieux comprendre la dynamique de
transmission du lyssavirus EBLV-1 chez les
chauves-souris. Comparaison et analyse
phylogénétique du génome complet du
Lyssavirus chez les chauves-souris
montrées infectées en France et en Europe
- Identification de marqueurs d'adaptation
et de virulence de deux biotypes du virus
rabique adaptés au chien et au renard
17 institutions,
9 pays
représentés
ToR: To collect, process, analyse, publish and disseminate epizootiological data
relevant to the designated pathogens or diseases
11. Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
12. Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
13. What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a) Articles published in peer-reviewed journals: 10
Cliquet F., Robardet E. and Picard-Meyer E.- Genetic strain modification of a live rabies virus vaccine widely used
in Europe for wildlife oral vaccination. Antiviral Res., 2013, 100, pp 84-89.
Fischer M., Wernike K., Freuling C.M., Muller T., Aylan O., Brochier B., Cliquet F., Vazquez-Moron S., Hostnik P.,
Huovilainen A., Isaksson M., Kooi E.A., Mooney J., Turcitu M.A., Rasmussen T.B., Revilla-Fernandez S., Smreczak
OIE Reference Laboratory Reports Activities, 2013
5
Rabies - Florence Cliquet - france
M., Fooks A.R., Marston D.A., Beer M. and Hoffmann B.- A step forward in molecular diagnostics of lyssaviruses Results of a ring trial among European laboratories. PlosOne, 2013, 8, pp 1-9.
Millan J., Chirife A.D., Kalema-Zikusoka G., Cabezon O., Muro J., Marco I., Cliquet F., Leon-Vizcaino L., Wasniewski
M., Almeria S. and Mugisha L.- Serosurvey of dogs for human, livestock, and wildlife pathogens, Uganda. Emerg.
infect. Dis., 2013, 19, pp 680-682.
Picard-Meyer E., Mrenoshki S., Milicevic V., Ilieva D., Cvetkovikj I., Cvetkovikj A., Krstevski K., Dzadzhovski I.,
Robardet E., Gagnev E., Iliev E., Plavsic B., Kirandjiski T. and Cliquet F.- Molecular characterisation of rabies virus
strains in the republic of Macedonia. Arch. Virol., 2013, 158, pp 237-240.
Picard-Meyer E., Servat A., Robardet E., Moinet M., Borel C. and Cliquet F.- Isolation of Bokeloh bat lyssavirus in
Myotis nattereri in France. Arch. Virol., 2013, 158, pp 2333-2340.
Robardet E., Andrieu S., Bruun Rasmussen T., Dobrostana M., Horton D.L., Hostnik P., Jaceviciene I., Juhasz T.,
Muller T., Mutinelli F., Servat A., Smreczak M., Vanek E., Vazquez-Moron S. and Cliquet F.- Comparative assay of
fluorescent antibody test results among twelve European National Reference Laboratories using various antirabies conjugates. J. Virol. Methods, 2013, 191, pp 88-94.
Robardet E., Ilieva D., Iliev E., Gagnev E., Picard-Meyer E. and Cliquet F.- Epidemiology and molecular diversity of
rabies viruses in Bulgaria. Epidemiol. Infect., 2013, pp 1-7. (in press)
Robardet E., Picard-Meyer E., Servat A. et Cliquet, F.- Étude de deux ans portant sur les résultats d’essais
interlaboratoires de diagnostic de la rage (test d’immunofluorescence, test d’inoculation à une culture cellulaire
pour le diagnostic de rage, test d’inoculation à la souris, techniques de PCR) : point de départ pour
l’harmonisation des méthodes. Euro Ref., 2013, pp 18-26.
Servat A. and Cliquet F.- Official batch control of rabies veterinary vaccines: current situation and perspectives in
the European Union. Altern. Lab. Anim., 2013, 41, pp 10-11.
Wasniewski M., Guiot A.L., Schereffer J.L., Tribout L., Mähar K. and Cliquet F.- Evaluation of an ELISA to detect
rabies antibodies in orally vaccinated foxes and raccoon dogs sampled in the field. J. Virol. Methods, 2013, 187,
pp 264-270.
b) International conferences: 14
Cliquet F.- Procedure for rabies antibody proficiency testing. Rabies serology meeting, Nancy, October, 1-2, 2013.
Cliquet, F.- Several tasks of the European Union Reference Laboratory (EURL) in the frame of rabies surveillance
and control in Europe and in Third countries. Workshop on regional policies to control rabies in the Southern
Mediterranean, Paris, 15-16 October 2013.
Cliquet F., Guiot A.L., Schereffer J.L., Tribout L., Mähar K. and Wasniewski M.- Evaluation of an ELISA to detect
rabies antibodies in wild (foxes and raccoon dogs) and domestic carnivores (dogs and cats). Rabies serology
meeting, Nancy, October, 1-2, 2013.
Millan J., Chirife A.D., Kalema-Zikusoka G., Cabezon O., Muro J., Marco I., Tico V., Cliquet, F., Leon-Vizcaino, L.,
Wasniewski, M., Almeria, S., Mugisha, L. 2013. Dogs in the interface: serosurvey of pathogens threatening human,
livestock and wildlife health. AITVM International Conference, 25-29 August 2013, Johannesburg, South Africa, 1
p.
Picard-Meyer E.- Comparison of different commercial SYBR Green® RT-PCR kits. 6th Workshop for Rabies NRLs,
Athens, Greece, 13-14 November 2013.
Picard-Meyer E. et Servat A.- Situation de la rage en Europe. Epidemiosurveillance programmée et
évenementielle des chauves-souris en France. Séminaire Rage chauve-souris, Luxembourg, 4 juillet 2013.
Picard-Meyer E., Servat A., Robardet E., Moinet M., Jouan D., Borel C. and Cliquet, F.- Bat rabies surveillance in
France. First report of Bokeloh Bat Lyssavirus in France. 5th European congress of virology, Lyon, September 1114, 2013.
Robardet E. and Cliquet F.- Inter-laboratory test for rabies diagnosis (FAT/ RTCIT/ RT-PCR/ qRT-PCR): 2013 session.
6th Workshop for Rabies NRLs, Athens, Greece, 13-14 November 2013.
6
OIE Reference Laboratory Reports Activities, 2013
Rabies - Florence Cliquet - france
Robardet E. and Cliquet F.- Inter-laboratory test on tetracycline and age determination on red fox tooth samples.
Second session (2012). 6th Workshop for Rabies NRLs, Athens, Greece, 13-14 November 2013.
Robardet E., Andrieu S. and Cliquet, F.- Comparative assay of fluorescent antibody test results among twelve
European National Reference Laboratories using various anti-rabies conjugates. Rabies in the Americas - RITA
XXIV, Toronto, Canada, October 27th-31st, 2013.
Servat A. and Cliquet, F.- Validation of the FAVN test as a serological potency assay to replace the in-vivo potency
tests of inactivated rabies vaccine for veterinary use. Rabies in the Americas - RITA XXIV, Toronto, Canada,
October 27th-31st, 2013.
Servat A. and Cliquet F.- Validation of the FAVN test as a serological potency assay to replace the in vivo potency
tests of inactivated rabies vaccine for veterinary use. 6th Workshop for Rabies NRLs, Athens, Greece, 13-14
November 2013.
Wasniewski M.- Satisfaction survey. EURL for rabies serology. Rabies serology meeting, Nancy, October, 1-2,
2013.
Wasniewski M., Kempff S., Labadie A., Rieder J., Schereffer J.L., Tribout L. and Cliquet F.- Results of the
collaborative study on a commercialised ELISA kit. Rabies serology meeting, Nancy, October, 1-2, 2013.
c) National conferences: 0
d) Other:
(Provide website address or link to appropriate information) 6
Rapports significatifs:
Cliquet F. and Wasniewski M.- Rabies serology proficiency testing. EU Reference laboratory for rabies serology,
Anses Nancy Laboratory for rabies and wildlife, 2013, RF-04/2013-V1-EN.
Cliquet F. and Wasniewski M.- Rabies serology proficiency testing. EU Reference laboratory for rabies serology,
Anses Nancy Laboratory for rabies and wildlife, 2013, RF-04/2013-V2-EN.
Robardet E., Andrieu S. and Cliquet F.- Inter-laboratory test 2013 for rabies diagnosis: Fluorescent antibody test
(FAT), Rapid tissue culture infection test (RTCIT), Reverse transcriptase polymerase chain reaction (RT-PCR) and
Real time RT-PCR. Anses Nancy EURL for Rabies, Malzéville, 2013, pp 1-46.
Robardet E. and Cliquet F.- Review of the analysis related to rabies diagnosis and follow-up of oral vaccination
performed in NRLs in 2012. Anses Nancy EURL for Rabies, Malzéville, 2013, pp 1-12.
Wasniewski M. and Cliquet F.- Collaborative study on the ELISA test produced by BioPro O.K. Servis. EU Reference
laboratory for rabies serology, Anses Nancy Laboratory for rabies and wildlife, 2013.
Picard-Meyer E., Fediaevsky A., Servat A. and Cliquet F.- Surveillance de la rage animale en France
métropolitaine. Bull Epidémiol Santé Anim – Alimentation, 2013, n°60, pp 12-18.
ToR: To provide scientific and technical training for personnel from OIE Member
Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member
Countries?
Yes
a) Technical visits: 2
OIE Reference Laboratory Reports Activities, 2013
7
Rabies - Florence Cliquet - france
b) Seminars: 3
c) Hands-on training courses: 16
d) Internships (>1 month): 0
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s) provided
with training
No. participants from the
corresponding country
c
Grèce
1
c
Indonésie
1
c
Kazakhstan
2
c
Montenegro
3
c
Bulgarie
1
c
Grèce
3
c
Royaume Uni/Mexique
1
c
Mali
1
a
Mexique
1
a et b
France
1
b et c
Chine
2
c
Mexique
1
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant
for the pathogen and the disease concerned
15. Does your laboratory have a Quality Management System certified according to an International Standard?
Yes
Quality management system adopted
ISO 17025
16. Is your laboratory accredited by an international accreditation body?
Yes
8
OIE Reference Laboratory Reports Activities, 2013
Rabies - Florence Cliquet - france
Test for which your laboratory is accredited
Accreditation body
Contrôle d’efficacité des vaccins antirabiques inactivés à
usage vétérinaire et humain
Mutual Joint Audit (EDQM) depuis le 05
mars 2008
Contrôle d’efficacité des vaccins vivants à usage oral pour la
faune sauvage
Mutual Joint Audit (EDQM) depuis le 30
janvier 2012
Séroneutralisation virale (recherche des anticorps
antirabiques)
COFRAC (depuis le 01 février 2008)
Méthode FAT (antigène du Lyssavirus)
COFRAC (depuis le 01 octobre 2012)
Méthode RTCIT (Lyssavirus)
COFRAC (depuis le 01 octobre 2012)
17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned?
No
(See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for
Aquatic Animals 2012, Chapter 1.1.1)
ToR: To organise and participate in scientific meetings on behalf of the OIE
18. Did your laboratory organise scientific meetings on behalf of the OIE?
No
19. Did your laboratory participate in scientific meetings on behalf of the OIE?
No
ToR: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same
pathogen or disease?
Yes
21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the
same pathogen or disease by organising or participating in proficiency tests?
Yes
OIE Reference Laboratory Reports Activities, 2013
9
Rabies - Florence Cliquet - france
Purpose of the
proficiency
tests: 1
Sérologie rage
chiens et chats
1
Role of your
Reference
Laboratory
(organiser/
participant)
Organisateur
No.
participants
Participating OIE Ref. Labs/ organising OIE Ref.
Lab.
65
Allemagne, Australie, Belgique, Brésil, Corée du
Sud, Croatie, Danemark, Espagne, Etats-Unis,
Finlande, France, Grèce, Hongrie, Israël, Italie,
Japon, Lettonie, Lituanie, Macédoine, Mexique,
Pays-Bas, Pérou, Pologne, Portugal, République
Slovaque, République Tchèque, Roumanie,
Royaume-Uni, Russie, Serbie-Monténégro,
Slovénie, Suède, Suisse, Turquie, Ukraine
validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.
22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific
research projects for the diagnosis or control of the pathogen of interest?
Yes
Title of the project or contract
Scope
Name(s) of relevant OIE Reference
Laboratories
Evaluation des performances
d'anticorps monoclonaux pour la
réalisation d'une nouvelle méthode
de diagnostic (DRIT)
Développer une méthode de
diagnostic alternative au FAT
Laboratoires de Référence de l'OIE
pour la rage : - d'Afrique du Sud d'Allemagne - du Canada - de France
- du Royaume Uni
Test interlaboratoire poru la
détermination d'un sérum
antirabique canin de référence
Proposer à l'OIE la
reconnaissance du sérum
canin comme sérum de
référence international OIE
Laboratoires de Référence de l'OIE
pour la rage : - d'Afrique du Sud d'Allemagne - des Etats-Unis - de
France - du Royaume Uni
ToR: To organise inter-laboratory proficiency testing with laboratories other than
OIE Reference Laboratories for the same pathogens and diseases to ensure
equivalence of results
23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than
OIE Reference Laboratories for the same disease?
Yes
Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
10
OIE Reference Laboratory Reports Activities, 2013
Rabies - Florence Cliquet - france
Purpose for inter-laboratory test comparisons1
Diagnostic de la rage par le test d’immunofluorescence
(test référencé par l’OIE) Organisateur et participant
Diagnostic de rage par la technique d’isolement viral sur
culture cellulaire (test référencé par l’OIE) Organisateur et
participant
PCR conventionnelle Organisateur et participant
PCR temps réel Organisateur et participant
No. participating
laboratories
Region(s) of participating
OIE Member Countries
43
Africa
Americas
Asia and Pacific
Europe
Middle East
25
Africa
Americas
Asia and Pacific
Europe
Middle East
27
Africa
Americas
Asia and Pacific
Europe
Middle East
23
Africa
Americas
Asia and Pacific
Europe
Middle East
ToR: To place expert consultants at the disposal of the OIE
24. Did your laboratory place expert consultants at the disposal of the OIE?
Yes
Kind of consultancy
Location
Commentaires sur le chapitre rage du code sanitaire pour les animaux
terrestres 2013
Nancy
Commentaire pour la révision du chapitre rage du manuel des tests de
diagnostic et vaccins pour les animaux terrestres 2013
Nancy
Subject
(facultative)
25. Additional comments regarding your report:
Question 1
Les lignes dans le tableau "RT-PCR temps réel" et "Titrage de virus rabique" font partie des Tests de diagnostic
direct, et non des Tests de diagnostic indirect (le tableau ne permet pas d'ajouter des lignes).
OIE Reference Laboratory Reports Activities, 2013
11

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