ผลของกรดไนไตรทrในการควบคุมเชื้อรา Colletotrichum capsici ที่ติดมากับ

Ÿ¨…°Š„¦—ŜئšrĜ„µ¦‡ª‡»¤ÁºÊ°¦µColletotrichum capsiciš¸É˜·—¤µ„´Á¤¨È—¡´œ›»r¡¦·„
œµŠ­µªš·¡ª¦¦–­·š›·­¤´˜·
š‡´—¥n°
æ‡Â°œÂš¦‡Ãœ­Áž}œž{®µ®œ¹ÉŠš¸É­Îµ‡´Äœ„µ¦Ÿ¨·˜¡¦·„Äœž¦³Áš«Åš¥
Ž¹ÉŠÁ„·—‹µ„ÁºÊ°¦µ
Colletotrichum spp. ­µ¤µ¦™Á…oµšÎµ¨µ¥Ÿ¨¡¦·„Å—oš»„¦³¥³Â¨³™nµ¥š°—Åž¥´ŠÁ¤¨È—¡´œ›»rŗo „µ¦‡ª‡»¤
æ‡Äœž{‹‹»´œœ·¥¤Äo­µ¦„ε‹´—ÁºÊ°¦µÁœºÉ°Š‹µ„Å—oŸ¨´—Á‹œÂ¨³¦ª—Á¦Èª ˜n‹³­nŠŸ¨„¦³š˜n°­»…£µ¡
…°ŠŸ¼oŸ¨·˜ ¨³Ÿ¼o¦·Ã£‡ ‹¹Š¤¸Âœª‡·—š¸É‹³œÎµ­µ¦š¸É¤¸‡ªµ¤ž¨°—£´¥¤µÄoĜ„µ¦‡ª‡»¤Ã¦‡ÄœÁ¤¨È—¡´œ›»r
ÎÁ—¸¥¤ÅœÅ˜¦šr NaNO2 Ĝ¦¼ž„¦—Áž}œ­µ¦ž¦»ŠÂ˜nŠš¸Éčo¥´¥´ÊŠÁºÊ°‹»¨·œš¦¸¥rĜ°»˜­µ®„¦¦¤°µ®µ¦Å—o
„µ¦š—¨°Šœ¸Ê‹¹Š¤¸ª´˜™»ž¦³­Š‡rÁ¡ºÉ°«¹„¬µ¦³—´‡ªµ¤Á…o¤…oœ…°Š„¦—Ŝئšr˜n°„µ¦¥´¥´ÊŠÁºÊ° C. capsici
isolate 158ci ĜÁ¤¨È—¡´œ›»r¡¦·„ (Capsicum annuum L.)¡´œ›»rµŠoµŠ ªµŠÂŸœ„µ¦š—¨°ŠÂ CRD
ץnÁ¤¨È—š¸ÉÁž}œÃ¦‡ 100% Ĝ­µ¦¨³¨µ¥„¦—Ŝئšr [NaNO2 in citric acid buffer (pH 2.5)] š¸É
¦³—´‡ªµ¤Á…o¤…oœ 0 (control) 100 200 250 300 350 ¨³ 400 mM Áž}œÁª¨µ 30 œµš¸ ¨³œÎµÁ¤¨È—Åž
ªµŠœ°µ®µ¦ PDA (potato dextrose agar) Áž}œÁª¨µ 7 ª´œ ¡ªnµ š¸É¦³—´‡ªµ¤Á…o¤…oœ 100 ¨³ 200
mM ­µ¤µ¦™¥´¥´ÊŠ„µ¦Á‹¦·Á˜·Ã˜…°ŠÁºÊ°Å—o 70 ¨³ 97% ˜µ¤¨Îµ—´ ¨³š¸É¦³—´‡ªµ¤Á…o¤…oœ ˜´ÊŠÂ˜n
250-400 mM ­µ¤µ¦™¥´¥´ÊŠ„µ¦Á‹¦·Á˜·Ã˜…°ŠÁºÊ°Å—o 100%
‡Îµ­Îµ‡´
:
­µ…µª·µ
:
ž{®µ¡·Á«¬ :
°µ‹µ¦¥rš¸Éž¦¹„¬µ :
žeš¸É¡·¤¡r
:
‹Îµœªœ®œoµ
:
¡¦·„ °œÂš¦‡Ãœ­ Á¤¨È—¡´œ›»r
Áš‡ÃœÃ¨¥¸Á¤¨È—¡´œ›»r¡º­ªœ
ž¦·µ˜¦¸ £µ‡ª·µ¡º­ªœ ‡–³Á„¬˜¦ „ε¡ŠÂ­œ ¤®µª·š¥µ¨´¥Á„¬˜¦«µ­˜¦r
—¦.žd¥³–´‘“r Ÿ„µ¤µ«
2553
11
Effect of Acidified Nitrite on Control of Seed-borne Colletotrichum capsici in Chili Pepper
Miss. Thippawan Sittisombut
Abstract
Anthracnose caused by Colletotrichum spp., is one of the most important diseases
of chili pepper production in Thailand. Anthracnose infects chili pepper at all growth stages
and transmits to seed. Conventionally, fungicides are widely used to control the disease, but
have adverse effect on health of farmers and consumers. Acidified nitrite (NaNO2) is a food
additive with known activity against microorganism. The objective of this study was to
determine the efficacy of acidified nitrite on control of seed-borne C. capsici isolate 158ci in
chili pepper (Capsicum annuum L.) cv.‘Bangchang’. The experiment was carried out in a
Completely Randomized Design (CRD). Soaking C. capsici isolate 158ci infected seeds in
acidified nitrite [NaNO2 in citric acid buffer (pH 2.5)] at 0 (control), 100, 200, 250, 300, 350 and
400 mM for 30 min, and then transferred the treated seeds on PDA (potato dextrose agar) for
7 days. Nitrite solution at 100 and 200 mM reduced disease incidences to 70 and 90%
respectively, and the 250-400 mM nitrite solution completely controlled seed-borne
anthracnose
Key words
Field
Degree
Advisor
Year
Pages
: chili pepper, anthracnose, seed
: Seed Technology
: B.S. (Agric.), Department of Horticulture, Faculty of Agriculture at
Kamphaeng Saen, Kasetsart University
: Dr. Piyanath Pagamas
: 2010
: 11