Cysteine & Cystine (Oxim & TMS formation)
Cysteineを測定するとCysteineとCystineの両者を検出する．Cysteine
がS-Sで結合し，Cystineとなる．
Cysteineは中性もしくは弱い塩基性溶液では酸化され，Cystineになる．
酸性溶液では安定(1)．
(x10,000,000)
1 00 220.00 (10.00)
1.00
218.00 (10.00)
0.75 411.00 (10.00)
System: GCMS-QP2010Plus, AOC20i+s
Column: InertCap 5MS/NP 0.25mm x 30m, df=0.25m
Column Temp.: 80ºC(2 min) - 15ºC/min - 330ºC(9 min)
Inj.: 230ºC, Split 25:1
Carrier Gas: He 39cm/s
S t
Septum
Purge:
P
5.0
5 0 mL/min
L/ i
Inj. Vol.: 1 L
Ion Source Temp.: 200ºC
Interface Temp.: 250ºC
Scan Range: m/z = 85 - 500
Scan Speed: 5000 u/s
Ribitol
(I.S.)
Cystine
_4TMS
Cysteine
_3TMS
0.50
0.25
0.00
10.0
10.5
11.0
11.5
12.0
12.5
13.0
13.5
14.0
14.5
15.0
15.5
16.0
%
220
100.0
O
NH
75.0
TMS
O
TMS
50.0
S
100
25.0
132
86
0.0
100
147
172
150
204
232
200
267
250
294
322
300
345
350
372
398 412 426
400
450
450
472 492
500
Cysteine_3TMS
(RI: 1568)
TMS
TMS
O
%
218
100.0
75.0
NH
O
146
S
S
50.0
100
25.0
0.0
266
232
132
86
100
178
150
203
200
297
288
250
300
322 338
350
378 396
400
O
TMS
411
438 457
450
(1) The MERCK INDEX THIRTEEN EDITION 2810
http://www.gls.co.jp/technique/metabolomics/index.html
485
500
Cystine_4TMS
(RI: 2325)
NH
O
Structures are created using Chemistry 4-D Draw
which is provided by ChemInnovation Software, Inc.
TMS
TMS

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