㸱 ㄪᰝ◊✲ 㸱࣭㸯 ሗᩥ 㸯㸧⇃ᮏ┴࡛║ᝈࡽ᳨ฟࡉࢀࡓࢹࣀ࢘ࣝࢫࡢ ศᏊᏛゎᯒ㸦 ᖺᗘ㹼 ᖺᗘ㸧 ྜྷᒸ ኴ ᪥㝰 㝣ኴ㑻 㸨 Ύ⏣ ┤Ꮚ 㸨 すᮧ ᾈ୍㸨 ཎ⏣ ㄔஓ せ᪨ ὶ⾜ᛶゅ⤖⭷⅖㸦EKC㸧➼ࡢ࡞ཎᅉ࢘ࣝࢫ࡛࠶ࡿࣄࢺ࣭ࢹࣀ࢘ࣝࢫ 㸦HAdV㸧ࡢὶ⾜ᆺࢆᢕᥱࡍࡿࡓࡵ㸪2008 ᖺᗘࡽ 2013 ᖺᗘࡢ㛫᳨ฟࡉࢀ ࡓ HAdV ࡢศᏊᏛゎᯒࢆ⾜ࡗࡓࠋࡑࡢ⤖ᯝ㸪12 ࡢᆺࡀ᳨ฟࡉࢀ㸪≉ 3 ᆺ㸪 4 ᆺ㸪8 ᆺ㸪37 ᆺ㸪53 ᆺ㸪54 ᆺ㸪ཬࡧ 56 ᆺࡢ᳨ฟ⋡ࡀ㧗ࡗࡓࠋࡲࡓ㸪4 ᆺ㸪 8 ᆺ㸪37 ᆺ㸪ཬࡧ 53 ᆺ࡛ὶ⾜ࡢᖺḟኚࡀࡳࡽࢀࡓࠋ ࡞࠾㸪52 ᆺ௨㝆ࡢ᪂ᆺ HAdV ࡛ࡣ㸪2008 ᖺᗘ 54 ᆺࡀ᳨ฟࡉࢀ㸪2010 ᖺ ᗘࡽ 53 ᆺཬࡧ 56 ᆺࡶ᳨ฟࡉࢀࡣࡌࡵࡓࠋࡇࢀࡽࡢ᪂ᆺ HAdV ࡼࡿ EKC ࡢ⮫ᗋ≧ࡣࡢᆺࡼࡾ㔜࡛㸪⒪ࡀ㛗ᘬࡃഴྥ࠶ࡗࡓࠋ ࣮࣮࢟࣡ࢻ㸸ὶ⾜ᛶゅ⤖⭷⅖㸪ࢹࣀ࢘ࣝࢫ㸪᪂ᆺࢹࣀ࢘ࣝࢫ ⛬ᗘࡢᢠ⾑Ύࡢ୰ヨ㦂࡛⾜ࢃࢀ࡚࠸ࡓࡀ㸪㏆ᖺࡣ㸪 ࡣࡌࡵ ᙜᡤࡣឤᰁⓎ⏕ືྥㄪᰝᴗࡋ࡚㸪║⛉ᐃⅬ PCRἲ࡛ቑᖜࡋࡓDNAࡢไ㝈㓝⣲ษ᩿ࣃࢱ࣮࡛ࣥᆺู ࡽᥦ౪ࡉࢀࡓ⮫ᗋ᳨యࡢ࢘ࣝࢫ᳨ᰝࢆ⾜ࡗ࡚࠸ࡿࠋ ࡍࡿ᪉ἲ 9㸧㸪PCRἲ࡛ቑᖜࡉࢀࡓhexson㑇ఏᏊࡢ㒊ศ㓄 ║⛉ᝈࡢ࠺ࡕ㸪㢮ឤᰁᐃⅬᢕᥱᝈࡢ୍ࡘ࡛࠶ ิࢆゎㄞࡍࡿࡇࡼࡾᆺูࡍࡿ᪉ἲ 10㸧㸪ቑᖜࡉࢀࡿ ࡿὶ⾜ᛶゅ⤖⭷⅖㸦௨ୗࠕEKCࠖ࠸࠺ࠋ㸧ࡣ㸪ࣄ DNAࡢࢧࢬࡀHAdVࡢᆺࡈ␗࡞ࡿࡼ࠺タィࡋࡓ ࢺ࣭ࢹࣀ࢘ࣝࢫ㸦௨ୗࠕHAdVࠖ࠸࠺ࠋ㸧ࡢឤᰁ ࣐ࣝࢳࣉࣞࢵࢡࢫ-PCRἲࡼࡗ࡚ᆺูࡍࡿ᪉ἲ 11㸧࡞ ࡼࡗ࡚Ⓨࡍࡿᝈ࡛࠶ࡿࠋHAdV ࡣ┤ᚄ⣙ 80nm㸪 ࡢ㑇ఏᏛⓗ᪉ἲࡶ⾜ࢃࢀ࡚࠸ࡿࠋࡋࡋ㸪᪂ᆺHAdV ṇ 20 㠃యᵓ㐀ࡢ࢚࣮ࣥ࣋ࣟࣈࢆᣢࡓ࡞࠸ 2 ᮏ㙐 DNA ࡣ㸪」ᩘࡢ࢘ࣝࢫࡢ㑇ఏᏊࡀ⤌ࡳኚࢃࡿࡇࡼࡾ⏕ ࢘ࣝࢫ࡛㸪2008 ᖺࡲ࡛ࡣ 51 ✀㢮ࡢᆺࡀᏑᅾࡋ㸪A ࡌࡓ࣓࢟ࣛ࢘ࣝࢫࡀከᩘྵࡲࢀ࡚࠾ࡾ㸪 53ᆺࡣhexon 㡿ᇦࡀ22ᆺ㸪fiber㡿ᇦࡀ8ᆺ㸪ཬࡧpenton㡿ᇦࡀ37ᆺ㸪54 㹼F ࡢ 6 ✀ศ㢮ࡉࢀ࡚࠸ࡓࠋࡋࡋࡑࡢᚋ㸪᪂ࡓ࡞ ࡞ࡾ㸪ࡉࡽ ᆺࡣfiber㡿ᇦࡀ8ᆺ㸪56ᆺࡣhexon㡿ᇦࡀ15ᆺ㸪pentonཬ ⫶⭠⅖⏤᮶ࡢ 52 ᆺࡀ G ✀ࡉࢀࡓࡓࡵ㸪✀ࡶ A㹼G ࡧfiber㡿ᇦࡀ9ᆺ࡛࠶ࡿࠋࡇࢀࡽࡢ࢘ࣝࢫࢆṇ☜ᆺ ᆺࡀḟࠎሗ࿌ࡉࢀ㸪⌧ᅾ 68✀㢮 㸧 ࠋHAdV ࡢ✀ᘬࡁ㉳ࡇࡍ ุᐃࡍࡿࡓࡵࡣ㸪ࣇࣝࢤࣀ࣒㸦⣙36,000bp㸧ࡢሷᇶ㓄 ᝈࡣ᫂░࡞㛵㐃ᛶࡀࡳࡽࢀ㸪EKC ࡽࡣᚑ᮶ D ✀ ิࢆỴᐃࡍࡿ᪉ἲࡀ᭱ࡶ☜ᐇ࡛࠶ࡿࡀ㸪⌧ᅾ㸪ᙜᡤࡀಖ ࡢ 8 ᆺ㸪19 ᆺ㸪37 ᆺ㸪E ✀ࡢ 4 ᆺ㸪ཬࡧ B ✀ࡢ 3 ᆺ ᭷ࡋ࡚࠸ࡿࢧ࣮ࣥ࢞ἲࡼࡿ࢟ࣕࣆ࣮ࣛࣜࢩ࣮ࢣࣥࢧ ࡢ 7 ✀࡞ࡗ࡚࠸ࡿ 㸧㸪㸧 ࡞ࡀࡼࡃ᳨ฟࡉࢀ࡚࠸ࡓࡀ㸪㏆ᖺࡣ 53 ᆺ 㸧㸧 ࣮࡛ࣇࣝࢤࣀ࣒ࡢሷᇶ㓄ิࢆỴᐃࡍࡿࡇࡣᐜ࡛᫆ࡣ 㸪54 ࡞࠸ࠋࡑࡇ࡛㸪⡆᫆ᆺูἲࡋ࡚㛤Ⓨࡉࢀ࡚࠸ࡿࢤࣀ࣒ ᆺ 㸧㸧㸪ཬࡧ 56 ᆺ 㸪࠸ࡗࡓ᪂ᆺ HAdV ࡀ᳨ฟࡉ ࡢhexon㸪fiberཬࡧpenton㡿ᇦࡢ୍㒊ࢆࡑࢀࡒࢀPCRἲ࡛ ࢀ㸪D ✀ศ㢮ࡉࢀࡿࡇࡀሗ࿌ࡉࢀ࡚࠸ࡿࠋ ቑᖜࡋ㸪ሷᇶ㓄ิࢆỴᐃࡍࡿ᪉ἲ 12㸧ࡼࡾᐇࡋࡓࠋ ║⛉㡿ᇦ࠾ࡅࡿHAdVࡢᆺྠᐃࡣ㸪ᚑ᮶㸪ศ㞳ࡉࢀ ᮏ◊✲࡛ࡣ㸪║⛉ࡢ⮫ᗋ᳨యࡽ᳨ฟࡉࢀࡓ HAdV ࡓ࢘ࣝࢫ║ᝈࢆ㉳ࡇࡋࡸࡍ࠸HAdVᑐࡍࡿ10✀ 㸨1 ᪥㝰║⛉་㝔 㸨2 ⌧⇃ᮏ┴┴༡ᗈᇦᮏ㒊ⰱᆅᇦ⯆ᒁಖ⚟♴⎔ቃ㒊 㸨3 ⌧⇃ᮏ┴┴ᗈᇦᮏ㒊 㜿⸽ᆅᇦ⯆ᒁಖ⚟♴⎔ቃ㒊 ࢆ୰ヨ㦂ࡸ㑇ఏᏛⓗ᪉ἲ࡛ᆺูࡋ㸪ὶ⾜ᆺࡢᖺḟኚ ✀㢮ࡢᆺࡢ HAdV ࡀ᳨ฟࡉࢀࡓࠋᆺࡢෆヂࡣ㸪37 ᆺࡀ ᆺෆࡢ㑇ఏᏛⓗኚ␗ཬࡧᆺࡼࡿ⮫ᗋ≧ࡢ┦㐪 55 ௳㸦28.6%㸧㸪56 ᆺࡀ 33 ௳㸦17.1%㸧㸪53 ᆺࡀ 21 ௳ ࡘ࠸࡚ゎᯒࢆ⾜ࡗࡓࡢ࡛ሗ࿌ࡍࡿࠋ 㸦10.9%㸧㸪3 ᆺࡀ 18 ௳㸦9.3%㸧㸪8 ᆺࡀ 17 ௳㸦8.8%㸧㸪 4 ᆺཬࡧ 54 ᆺࡀྛ 16 ௳㸦8.3%㸧㸪11 ᆺࡀ 5 ௳㸦2.6%㸧㸪 ᮦᩱཬࡧ᪉ἲ 19 ᆺࡀ 2 ௳㸦1.0%㸧㸪1 ᆺ㸪6 ᆺཬࡧ 34 ᆺࡀྛ 1 ௳㸦0.5%㸧㸪 2008 ᖺ 4 ᭶ࡽ 2014 ᖺ 3 ᭶ࡢ㛫㸪EKC ࠶ࡿ࠸ࡣ 53 ᆺ㸪54 ᆺཬࡧ 56 ᆺࡣྜィ 70 ௳㸦36.4%㸧࡛࠶ࡗࡓࠋ 㸧᳨ᰝᮦᩱ ᆺู᫂㸦NT㸧ࡀ 6 ௳㸦3.1%㸧࡛࠶ࡾ㸪᪂ᆺ࡛࠶ࡿ ⤖⭷⅖➼ࡢ⮫ᗋデ᩿ྡ࡛║⛉ᐃⅬࡽᥦ౪ࡉࢀࡓ⤖⭷ ⮫ᗋデ᩿ྡูࡳࡿ㸪EKC デ᩿ࡉࢀࡓ 257 ௳࡛ࡣ㸪 ࡠࡄ࠸ᾮ 460 ௳ࢆ᳨ᰝᮦᩱࡋࡓࠋ 163 ௳㸦63.4%㸧ࡽ HAdV ࡀ᳨ฟࡉࢀ㸪37 ᆺࢆ➹㢌 㸪3 ᆺ㸪4 ᆺ㸪8 ᆺ㸪53 ᆺ㸪54 ᆺཬࡧ 56 ᆺࡢ᳨ฟ⋡ 㸧᳨ᰝ᪉ἲ ձ࢘ࣝࢫศ㞳ཬࡧ୰ヨ㦂ࡼࡿᆺู㸸A549 ⣽ ࡀ㧗ࡗࡓࠋ୍᪉㸪⤖⭷⅖➼デ᩿ࡉࢀࡓ 203 ௳࡛ࡣ㸪 ⬊ཬࡧ RD-18S ⣽⬊ࢆ⏝࠸㸪HAdV ࡢศ㞳ࢆ⾜ࡗࡓࠋ 29 ௳㸦14.3%㸧ࡽ HAdV ࡀ᳨ฟࡉࢀ㸪56 ᆺࡢ᳨ฟ⋡ ࡑࡢᚋ㸪HAdV ᢠ⾑Ύ㸦1㹼8 ᆺ㸪11 ᆺ㸪19 ᆺཬࡧ 37 ࡀẚ㍑ⓗ㧗ࡗࡓ㸦⾲ 1㸧ࠋ ᆺ㸸ࢹࣥ࢝⏕◊ࡲࡓࡣᅜ❧ឤᰁ◊✲ᡤ㓄ᕸ㸧ࡼࡿ ୰ヨ㦂࡛ᆺࢆỴᐃࡋࡓࠋࡲࡓ㸪ୗグࡢ㑇ఏᏊ᳨ᰝἲ 㸧ὶ⾜ᆺࡢᖺḟኚ 4 ᆺ㸪8 ᆺ㸪37 ᆺཬࡧ 53 ᆺὶ⾜ࡢᖺḟኚࡀࡳࡽ ࡶే⏝ࡋ࡚ᆺูࢆ⾜ࡗࡓࠋ ࢀࡓࠋ4 ᆺࡣ 2012 ᖺᗘ㸪8 ᆺࡣ 2013 ᖺᗘ᳨ฟ⋡ࡀቑ ղ3&5 ἲࡼࡿ㑇ఏᏊ᳨ᰝཬࡧࢲࣞࢡࢺࢩ࣮ࢡ࢚ ࣥࢫἲࡼࡿᆺู㸸2008 ᖺ 4 ᭶ࡽ 2010 ᖺ 7 ᭶ࡲ࡛ ຍࡋࡓࠋ37 ᆺࡣẖᖺᗘ᳨ฟࡉࢀ࡚࠸ࡿࡀ㸪≉ 2008 ࡢဗ㢌ࡠࡄ࠸ᾮ 130 ௳ࡽศ㞳ࡉࢀࡓᰴࡣ୰ヨ㦂ࡢ ᖺᗘ㹼2009 ᖺᗘཬࡧ 2013 ᖺᗘࡢ᳨ฟ⋡ࡀ㧗ࡗࡓࠋ ࡳ࡛ᆺูࢆ⾜ࡗࡓࠋṧࡾࡢဗ㢌ࡠࡄ࠸ᾮ 330 ௳ࡣ㸪࢘ 53 ᆺࡣ 2010 ᖺᗘࡽ᳨ฟࡉࢀጞࡵࡓࡀ㸪ึᖺᗘࡀ᭱ ࣝࢫศ㞳୪⾜ࡋ࡚㸪ဗ㢌ࡠࡄ࠸ᾮࡽ┤᥋ DNA ࡶ㧗⋡᳨ฟࡉࢀࡓ㸦⾲ 2㸪ᅗ 1㸧ࠋ ࢆᢳฟࡋ㸪HAdV ࡢࢫࢡ࣮ࣜࢽࣥࢢ⏝ࡋ࡚ Xu ࡽ 13㸧 㸧᭶᳨ูฟ≧ἣ ࡢ Ad1/Ad2 ࣉ࣐࣮ࣛࡼࡿ PCR ἲ࡛ࢤࣀ࣒ DNA ࡢ ྛᆺࡢ᳨ฟᩘࡣᖺᗘࡼࡾ␗࡞ࡾ㸪ᐃࡲࡗࡓࣃࢱ࣮ hexon C4 㡿ᇦࢆቑᖜᚋ㸪࣮࢞ࣟࢫࢤࣝ㟁ẼὋືࡼ ࣥࡣࡳࡽࢀ࡞ࡗࡓ㸦ᅗ 2㸧ࠋ ࡾ┠ⓗ㑇ఏᏊࡢቑᖜࣂࣥࢻ㸦482bp㸧ࢆ☜ㄆࡋࡓࠋ࡞࠾㸪 ᖺ㱋᳨ูฟ≧ἣ ࢘ࣝࢫࡣศ㞳࡛ࡁࡎ㸪㑇ఏᏊࡢࡳࡀ᳨ฟࡉࢀࡓ᳨య 20 ௦㹼40 ௦ࢆࣆ࣮ࢡࡋ࡚㸪0 ṓࡽ 70 ṓ௨ୖࡲ ࡸ㸪୰ἲ࡛ศ㞳ᰴࡢᆺỴᐃࡀ࡛ࡁࡎ㸪᪂ᆺ HAdV ࡀ ࡛ᖜᗈ࠸ᖺ㱋ᒙࡽ᳨ฟࡉࢀࡓࡀ㸪92.7㸣㸦178 ௳/192 ࢃࢀࡓᰴࡣ㸪ᗘ Takeuchi ࡽ 14㸧 ௳㸧ࡀ 20 ௦௨ୖࡽࡢ᳨ฟ࡛࠶ࡾ㸪20 ṓᮍ‶ࡽࡣ ࡢ S28/S52 ࣉࣛ ࣐࣮ࡼࡿ PCR ἲ࡛ hexon loop1 㡿ᇦࢆ㸪Xu ࡽ 13㸧 㠀ᖖᑡ࡞ࡗࡓ㸦ᅗ 3㸧ࠋ ࡢ 㸧⣔⤫ᶞゎᯒ 6 ᑐࡢ✀≉␗ⓗࣉ࣐࣮ࣛࢆ⏝࠸ࡓ࣐ࣝࢳࣉࣞࢵࢡࢫ PCR ἲ࡛ fiber 㡿ᇦࢆ㸪ཬࡧ Fujimoto ࡽ 14㸧ࡢ penton PCR ࢲࣞࢡࢺࢩ࣮ࢣࣥࢫἲ࡛ሷᇶ㓄ิࢆỴᐃࡋࡓ୍㒊 ⏝ࣉ࣐࣮࡛ࣛ penton 㡿ᇦࢆࡑࢀࡒࢀࡢ PCR ᮲௳࡛ ࡢᰴࡘ࠸࡚㸪hexon㸪fiber㸪ཬࡧ penton 㡿ᇦࡢศᏊ ቑᖜࡋࡓࠋቑᖜࣂࣥࢻࢆ⢭〇ᚋ㸪ࢲࣞࢡࢺࢩ࣮ࢡ࢚ ⣔⤫ᶞゎᯒࢆ⾜ࡗࡓࠋࡑࡢ⤖ᯝ㸪ྛᆺࡈ᫂░࡞ࢡ ࣥ ࢫ ἲ ࡛ ሷ ᇶ 㓄 ิ ࢆ Ỵ ᐃ ࡋ 㸪 BLAST ᳨ ⣴ ཬ ࡧ ࣛࢫࢱ࣮ࢆᙧᡂࡋࡓࠋࢡࣛࢫࢱ࣮ෆࡢሷᇶ㓄ิࡢ㐪࠸ MEGA5.0 ࢆ⏝࠸ࡓ㏆㞄⤖ྜἲࡼࡿศᏊ⣔⤫ᶞ࡛ᆺ ࡣࢇㄆࡵࡽࢀ࡞ࡗࡓࡀ㸪4 ᆺ㸦fiber 㡿ᇦ㸧㸪8 ࢆุᐃࡋࡓࠋ ᆺ㸦hexon 㡿ᇦ㸪penton 㡿ᇦ㸧ཬࡧ 56 ᆺ㸦penton 㡿ᇦ㸧 ճ⮫ᗋሗࡢ㞟㸸HAdV ࡢᆺࡼࡿ⮫ᗋ≧➼ࡢ ࡛㸪᳨ฟᖺᗘࡼࡾࢃࡎ࡞㐪࠸ࡀࡳࡽࢀ㸪ࢧࣈࢡࣛ ┦㐪ࢆㄪᰝࡍࡿࡓࡵ㸪᳨యῧࡢᝈ⪅ሗࢆཧ⪃ࡍ ࢫࢱ࣮ࢆᙧᡂࡋࡓࠋ࡞࠾㸪࣓࢟ࣛ࢘ࣝࢫࡉࢀࡿ 53 ࡿࡶ㸪ཎయᐃⅬ࡛࠶ࡿ║⛉་㝔ࡽ┤᥋ሗ ᆺࡣ hexon 㡿ᇦࡀ 22 ᆺ㸪fiber 㡿ᇦࡀ 8 ᆺཬࡧ penton ࢆ㞟ࡋࡓࠋ 㡿ᇦࡀ 37 ᆺྠ୍ࡢࢡࣛࢫࢱ࣮ศ㢮ࡉࢀࡓࠋࡲࡓ㸪 54 ᆺࡣ fiber 㡿ᇦࡢࡳ 8 ᆺࡢࢧࣈࢡࣛࢫࢱ࣮ࡋ࡚ศ 㢮ࡉࢀ㸪56 ᆺࡣ hexon 㡿ᇦࡀ 15 ᆺ㸪fiber 㡿ᇦཬࡧ penton ᳨ᰝᡂ⦼ 㸧+$G9 ࡢ᳨ฟ≧ἣ 㡿 ᇦ ࡀ 9 ᆺ ྠ ୍ࡢ ࢡ ࣛࢫ ࢱ ࣮ ศ 㢮ࡉ ࢀ ࡓ 㸦 ᅗ 4-a,b,c㸧ࠋ ║⛉ࡢ⮫ᗋ᳨య 460 ௳୰ 192 ௳㸦41.7㸣㸧ࡽ㸪12 ⾲㸯 ⮫ᗋデ᩿ྡูࢹࣀ࢘ࣝࢫ᳨ฟ≧ἣ㸦㹼 ᖺᗘ㸧 ὶ⾜ᛶ ⤖⭷⅖➼ ྜィ ゅ⤖⭷⅖ ᳨యᩘ +$G9 ᆺ 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 +$G9 ᆺ 㸦㸧 㸦㸧 㸦㸧 +$G917 㸦㸧 㸦㸧 㸦㸧 ᳨ฟᩘ ᳨ฟ⋡ 㸣 㸣 㸣 ͤ㸦 㸧ෆࡣྛᆺࡢ᳨ฟᩘ/᳨ฟᩘ㸦㸣㸧 ᳨యᩘ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G9 ᆺ +$G917 ᳨ฟᩘ ᳨ฟ⋡㸦㸧 ⾲㸰 ᆺู࣭ᖺᗘูࢹࣀ࢘ࣝࢫ᳨ฟᩘ ᖺᗘ ᖺᗘ ᖺᗘ ᖺᗘ ᖺᗘ ᖺᗘ 㸪 ྜィ ᅗ㸯 ᆺู࣭ᖺᗘูࢹࣀ࢘ࣝࢫ᳨ฟྜ ᅗ㸱 ᖺ㱋ูࢹࣀ࢘ࣝࢫ᳨ฟ≧ἣ ᅗ㸰 ᭶ูࢹࣀ࢘ࣝࢫ᳨ฟ≧ἣ AB605243 AdV-53 AB330103 AdV-22 20131604 Kumamoto/2013 20131529 Kumamoto/2013 20121537 Kumamoto/2012 20121515 Kumamoto/2012 20121506 Kumamoto/2012 20101548 Kumamoto/2010 20101544 Kumamoto/2010 㻥㻥 20101543 Kumamoto/2010 20101527 Kumamoto/2010 20101526 Kumamoto/2010 20101525 Kumamoto/2010 20101524 Kumamoto/2010 20101523 Kumamoto/2010 20101522 Kumamoto/2010 㻡 20101519 Kumamoto/2010 20101507 Kumamoto/2010 20101504 Kumamoto/2010 AB330130 AdV-49 20131586 Kumamoto/2013 AB330089 AdV-8 20131584 Kumamoto/2013 㻢㻠 20131559 Kumamoto/2013 20131555 Kumamoto/2013 㻤 㻥㻥 20131544 Kumamoto/2013 20111532 Kumamoto/2011 20121540 Kumamoto/2012 AB330097 AdV-16 㻝㻡 㻟㻜 AB330085 AdV-4 20121536 Kumamoto/2012 㻥㻥 20121561 Kumamoto/2012 20121577 Kumamoto/2012 㻣㻝 20121581 Kumamoto/2012 㻢㻠 20121584 Kumamoto/2012 20121596 Kumamoto/2012 20131511 Kumamoto/2013 20131515 Kumamoto/2013 㻣㻢 20121546 Kumamoto/2012 㻥㻥 20121592 Kumamoto/2012 AB330100 AdV-19 AB330107 AdV-26 㻡㻠 AB330111 AdV-30 20121545 Kumamoto/2012 㻢㻞 20121576 Kumamoto/2012 㻥㻥 20131531 Kumamoto/2013 㻟㻢 20131562 Kumamoto/2013 㻥㻥 20131575 Kumamoto/2013 20131581 Kumamoto/2013 AB330118 AdV-37 20121538 Kumamoto/2012 AB448770 AdV-54 20121532 Kumamoto/2012 20121526 Kumamoto/2012 㻥㻥 20121523 Kumamoto/2012 20121517 Kumamoto/2012 20081583 Kumamoto/2008 20081579 Kumamoto/2008 㻤㻡 20081577 Kumamoto/2008 20081576 Kumamoto/2008 AB330090 AdV-9 AB330113 AdV-32 㻤㻞 AB330104 AdV-23 AB330110 AdV-29 20101505 Kumamoto/2010 㻡㻠 20101512 Kumamoto/2010 20101513 Kumamoto/2010 20101529 Kumamoto/2010 20101533 Kumamoto/2010 㻥㻥 20101535 Kumamoto/2010 㻣㻝 20101536 Kumamoto/2010 20101537 Kumamoto/2010 20101563 Kumamoto/2010 20121516 Kumamoto/2012 20121518 Kumamoto/2012 20121524 Kumamoto/2012 20121542 Kumamoto/2012 㻥㻥 20121563 Kumamoto/2012 20121569 Kumamoto/2012 20121570 Kumamoto/2012 20121594 Kumamoto/2012 20131502 Kumamoto/2013 20131505 Kumamoto/2013 20131513 Kumamoto/2013 20131520 Kumamoto/2013 20131532 Kumamoto/2013 20131612 Kumamoto/2013 20131621 Kumamoto/2013 AB330096 AdV-15 HM770721 AdV-56 AB330088 AdV-7 20101542 Kumamoto/2010 20131508 Kumamoto/2013 㻥㻥 20131523 Kumamoto/2013 20131527 Kumamoto/2013 㻣㻤 20131541 Kumamoto/2013 20131558 Kumamoto/2013 AB330084 AdV-3 DQ923122 AdV-52 DQ874353 AdV-55 AB330082 AdV-1 㻢㻢 AB330092 AdV-11 㻠㻞 㻜㻚㻝 ᅗ D KH[RQ 㡿ᇦࡢศᏊ⣔⤫ᶞ 㻞㻞 ᆺ㻌 䠄㻡㻟 ᆺ䠅㻌 㻤 ᆺ㻌 㻠 ᆺ㻌 㻝㻥 ᆺ㻌 㻟㻣 ᆺ㻌 㻡㻠 ᆺ㻌 㻝㻡 ᆺ㻌 䠄㻡㻢 ᆺ䠅㻌 㻟 ᆺ㻌 20121540 Kumamoto/2012 20131584 Kumamoto/2013 20131565 Kumamoto/2013 20131559 Kumamoto/2013 20131555 Kumamoto/2013 20131552 Kumamoto/2013 20131549 Kumamoto/2013 20131548 Kumamoto/2013 20131546 Kumamoto/2013 20131545 Kumamoto/2013 20131544 Kumamoto/2013 㻟㻝 20111532 Kumamoto/2011 AB746853 AdV-8 20131586 Kumamoto/2013 AB605243 AdV-53 20101504 Kumamoto/2010 20101507 Kumamoto/2010 20101522 Kumamoto/2010 20101543 Kumamoto/2010 㻣㻟 20101544 Kumamoto/2010 20101548 Kumamoto/2010 20121506 Kumamoto/2012 20131529 Kumamoto/2013 20131604 Kumamoto/2013 20101519 Kumamoto/2010 㻠㻢 20101523 Kumamoto/2010 20101524 Kumamoto/2010 㻡㻣 20101525 Kumamoto/2010 20101527 Kumamoto/2010 20101526 Kumamoto/2010 AB448770 AdV-54 20081576 Kumamoto/2008 20081577 Kumamoto/2008 㻤㻞 20081579 Kumamoto/2008 20081583 Kumamoto/2008 20121517 Kumamoto/2012 㻣㻝 AJ854486 AdV-9 HM770721 AdV-56 20101505 Kumamoto/2010 20101512 Kumamoto/2010 20101513 Kumamoto/2010 20101529 Kumamoto/2010 20101533 Kumamoto/2010 20101535 Kumamoto/2010 20101536 Kumamoto/2010 20101537 Kumamoto/2010 20121563 Kumamoto/2012 㻥㻞 20121515 Kumamoto/2012 20121516 Kumamoto/2012 㻟㻥 20101563 Kumamoto/2010 20121569 Kumamoto/2012 20121570 Kumamoto/2012 20121594 Kumamoto/2012 20131502 Kumamoto/2013 20131505 Kumamoto/2013 20131520 Kumamoto/2013 20131532 Kumamoto/2013 20131621 Kumamoto/2013 JN226748 AdV-15 㻢㻤 FJ619037 AdV-22 AB448771 AdV-19 20121592 Kumamoto/2012 AB448777 AdV-37 㻞㻟 㻠㻣 20121546 Kumamoto/2012 20121577 Kumamoto/2012 㻥㻣 20131531 Kumamoto/2013 20131534 Kumamoto/2013 20131562 Kumamoto/2013 20131570 Kumamoto/2013 㻢㻜 20131575 Kumamoto/2013 20131578 Kumamoto/2013 20131581 Kumamoto/2013 20131585 Kumamoto/2013 20131587 Kumamoto/2013 20131589 Kumamoto/2013 20131618 Kumamoto/2013 㻝㻜㻜 20121536 Kumamoto/2012 20121561 Kumamoto/2012 20121576 Kumamoto/2012 㻝㻜㻜 20121581 Kumamoto/2012 20121584 Kumamoto/2012 㻝 20121596 Kumamoto/2012 㻜 20131511 Kumamoto/2013 㻞 20131515 Kumamoto/2013 㻟 AY594253 AdV-4 㻥 AB920438 AdV-3 20101542 Kumamoto/2010 20131508 Kumamoto/2013 20131523 Kumamoto/2013 㻝㻜㻜 20131527 Kumamoto/2013 20131541 Kumamoto/2013 20131543 Kumamoto/2013 20131558 Kumamoto/2013 JX173085 AdV-1 JX123029 AdV-55 AY598970 AdV-11 㻣㻥 DQ923122 AdV-52 㻠㻥 㻜㻚㻞 ᅗ E ILEHU 㡿ᇦࡢศᏊ⣔⤫ᶞ 㻤 ᆺ㻌 㻤 ᆺ㻌 䠄㻡㻟 ᆺ䠅㻌 㻡㻠 ᆺ㻌 㻥 ᆺ㻌 䠄㻡㻢 ᆺ䠅㻌 㻝㻥 ᆺ㻌 㻟㻣 ᆺ㻌 㻠 ᆺ㻌 㻟 ᆺ㻌 㻟㻤 㻞 20101543 Kumamoto/2010 20101527 Kumamoto/2010 20101544 Kumamoto/2010 20101548 Kumamoto/2010 20121537 Kumamoto/2012 20131529 Kumamoto/2013 20131604 Kumamoto/2013 20131605 Kumamoto/2013 AB605243 AdV-53 20121577 Kumamoto/2012 20131618 Kumamoto/2013 20131587 Kumamoto/2013 20131585 Kumamoto/2013 20131581 Kumamoto/2013 㻥㻟 20131578 Kumamoto/2013 20131575 Kumamoto/2013 20131573 Kumamoto/2013 20131570 Kumamoto/2013 20131562 Kumamoto/2013 20131534 Kumamoto/2013 20131531 Kumamoto/2013 AB448777 AdV-37 20101526 Kumamoto/2010 20101525 Kumamoto/2010 㻟㻜 20101524 Kumamoto/2010 20101523 Kumamoto/2010 20101522 Kumamoto/2010 20101519 Kumamoto/2010 20101507 Kumamoto/2010 20101504 Kumamoto/2010 㻥㻤 AB448771 AdV-19 20121546 Kumamoto/2012 AB746853 AdV-8 20131544 Kumamoto/2013 20131545 Kumamoto/2013 20131546 Kumamoto/2013 㻟㻠 20131547 Kumamoto/2013 㻞㻢 20131548 Kumamoto/2013 20131549 Kumamoto/2013 㻥㻞 20131552 Kumamoto/2013 20131555 Kumamoto/2013 20131557 Kumamoto/2013 20131559 Kumamoto/2013 20131565 Kumamoto/2013 20131567 Kumamoto/2013 㻜 20131556 Kumamoto/2013 㻜 20111532 Kumamoto/2011 㻜 20131584 Kumamoto/2013 㻞 㻥㻠 AY598970 AdV-11 KF268123 AdV-3 20101542 Kumamoto/2010 20131508 Kumamoto/2013 㻝㻤 20131523 Kumamoto/2013 20131527 Kumamoto/2013 㻥㻤 20131541 Kumamoto/2013 20131543 Kumamoto/2013 20131558 Kumamoto/2013 AY594253 AdV-4 20121561 Kumamoto/2012 20121576 Kumamoto/2012 㻤㻢 20121581 Kumamoto/2012 20121584 Kumamoto/2012 㻣㻢 20121596 Kumamoto/2012 20131511 Kumamoto/2013 20131515 Kumamoto/2013 㻥㻥 JN226748 AdV-15 JX123029 AdV-55 DQ923122 AdV-52 JX173085 AdV-1 20121532 Kumamoto/2012 20121538 Kumamoto/2012 20121529 Kumamoto/2012 20121526 Kumamoto/2012 㻥㻠 20121517 Kumamoto/2012 20081583 Kumamoto/2008 㻟㻟 20081579 Kumamoto/2008 20081577 Kumamoto/2008 20081576 Kumamoto/2008 AB448770 AdV-54 20101537 Kumamtoo/2010 㻥㻥 20101563 Kumamoto/2010 20121569 Kumamoto/2012 20121570 Kumamoto/2012 20121594 Kumamoto/2012 20131502 Kumamoto/2013 㻢㻟 20131505 Kumamoto/2013 20131513 Kumamoto/2013 20131520 Kumamoto/2013 20131612 Kumamoto/2013 20131621 Kumamoto/2013 㻜 20121524 Kumamoto/2013 HM770721 AdV-56 㻜 AJ854486 AdV-9 20101505 Kumamoto/2010 㻠 20101512 Kumamoto/2010 20101513 Kumamoto/2010 20101529 Kumamoto/2010 㻟㻥 20101533 Kumamoto/2010 20101535 Kumamoto/2010 20101536 Kumamoto/2010 20121542 Kumamoto/2013 ᅗ F SHQWRQ 㡿ᇦࡢศᏊ⣔⤫ᶞ 㻟㻣 ᆺ㻌 䠄㻡㻟 ᆺ䠅㻌 㻌㻌 㻟㻣 ᆺ 㻟㻣 ᆺ㻌 䠄㻡㻟 ᆺ䠅㻌 㻝㻥 ᆺ㻌 㻤 ᆺ㻌 㻟 ᆺ㻌 㻠 ᆺ㻌 㻡㻠 ᆺ㻌 㻥 ᆺ㻌 䠄㻡㻢 ᆺ䠅㻌 ⾲䠏㻌 䜴䜲䝹䝇ศ㞳䛸 㻼㻯㻾 ἲ䛾ẚ㍑䠄௳ᩘ䠅㻌 㻌㻌 㻼㻯㻾䠄䠇䠅㻌 㻼㻯㻾䠄䠉䠅㻌 ྜィ㻌 ศ㞳䠄䠇䠅㻌 㻝㻞㻟㻌 㻜㻌 㻝㻞㻟㻌 ศ㞳䠄䠉䠅㻌 㻞㻣㻌 㻝㻤㻜㻌 㻞㻜㻣㻌 ྜィ㻌 㻝㻡㻜㻌 㻝㻤㻜㻌 㻟㻟㻜㻌 㛗ᖺࢇ᳨ฟࡉࢀ࡞ࡗࡓ 8 ᆺࡼࡿ EKC ࡀ㸪 2011 ᖺᕝᓮᕷ࡛✺↛ᩓⓎᛶ㞟ᅋⓎ⏕ࡋࡓࢆ ሗ࿌ࡋ࡚࠸ࡿࡇࡽ㸪ࡇࢀࡽࡢᆺࡣ㸪ࡲࡔ EKC ࡢ㔜 せ࡞ཎᅉ࢘ࣝࢫ࡛࠶ࡾ㸪㞟ᅋචࡢపୗࡼࡾ㸪 ὶ⾜ࢆ㉳ࡇࡍྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ ᪂ᆺ HAdV ࡢ࠺ࡕ㸪53 ᆺࡣ 2010 ᖺᗘึࡵ᳨࡚ฟ ࡉࢀࡓࡀ㸪᳨ฟ௳ᩘࡢ 36.3㸣㸦12 ௳/33 ௳㸧ࢆ༨ࡵ㸪 㸧᪂ᆺ +$G9 ࡢ୰ヨ㦂࠾ࡅࡿᕪᛂ ᪂ᆺ HAdV ࡣ࠸ࡎࢀࡢᆺࡶ 8 ᆺᕪᛂࡀㄆࡵࡽ ࡑࡢᚋẖᖺᗘ᳨ฟࡉࢀ࡚࠸ࡿࠋkaneko ࡽ 3㸧ࡣ㸪53 ᆺ ࢀ㸪HAdV ᢠ⾑Ύ࡛⣽⬊ኚᛶຠᯝ㸦CPE㸧ࡀᢚไࡉࢀ ࡣ 1996 ᖺࡽᅜෆ࡛ᬑ㏻᳨ฟࡉࢀࡿࡼ࠺࡞ࡗࡓ ࡿഴྥࢆ♧ࡋࡓࠋࡋࡋ㸪ᐇ㝿ࡢ 8 ᆺ HAdV ẚ㍑ࡍ ࡀ㸪ᐇ㝿ࡣ 1980 ᖺ௦ EKC ࡽศ㞳ࡉࢀ࡚࠸ࡓ㢮ఝ ࡿᢚไࡢᙅ࠸ᰴࡀከࡃ㸪୰ࡣࢇᢚไࡢㄆࡵ ࢘ࣝࢫࡽ㐍ࡋࡓྍ⬟ᛶࡀ࠶ࡿ㏙࡚࠸ࡿࠋ 54 ᆺࡣ㸪Ishiko ࡽ 5㸧㸪16㸧ࡼࡿ㸪2000 ᖺ⚄ᡞཬ ࡽࢀ࡞࠸ᰴࡶほᐹࡉࢀࡓࠋ ࡧᯇࡢ EKC ᝈ⪅ࡽ᳨ฟࡉࢀ㸪2001 ᖺࡣᮏ┴ࡢ ࢘ࣝࢫศ㞳 3&5 ἲࡢẚ㍑ ᝈ⪅ࡽࡶ᳨ฟࡉࢀ࡚࠸ࡿࠋࡑࡢᚋ㸪ᅇࡢㄪᰝ࡛ ୧᪉ࡢ᳨ᰝἲࢆే⏝ࡋࡓ⮫ᗋ᳨య 330 ௳ࡘ࠸࡚㸪 ᳨ᰝἲࡈࡢ᳨ᰝᡂ⦼ࢆẚ㍑ࡍࡿ㸪࢘ࣝࢫศ㞳࡛ 2008 ᖺ᳨ฟࡉࢀࡿࡲ࡛㸪ࡑࡢືྥࡣ࡛᫂࠶ࡿࡀ㸪 ࡣ 123 ௳㸦37.3㸣㸧ࡽศ㞳ࡉࢀ㸪PCR ἲ࡛ࡣࡑࢀࡽ 54 ᆺࡣ 8 ᆺࡢᢠ⾑Ύᕪࡀ࠶ࡾ㸪8 ᆺྠᵝ㸪⣽⬊ᇵ ࢆࡍ࡚ྵࡴ 150 ௳㸦45.4㸣㸧ࡽ᳨ฟࡉࢀࡓ㸦⾲ 3㸧ࠋ 㣴ࡼࡿ CPE ࡢⓎ⌧ࡀ㐜࠸ࡇࡽ㸪8 ᆺㄗྠᐃࡉ ࢀ࡚࠸ࡿྍ⬟ᛶࡀ⪃࠼ࡽࢀࡓࠋ ࡲࡓ㸪࢘ࣝࢫศ㞳ࡢࡳ᳨࡛ᰝࡋࡓ 2008 ᖺ 4 ᭶ࡽ 56 ᆺࡣ㸪Kaneko ࡽ 6㸧ࡼࡿ㸪ᮏ┴ࢆྵࡴᅜෆ 7 2010 ᖺ 7 ᭶ࡲ࡛ࡢ 130 ௳ࡽ 42 ᰴࡢ HAdV ࡀศ㞳ࡉ ࢀ㸪ࡇࢀࡽࡢ᳨యࡣࡍ࡚ PCR 㝧ᛶ࡛࠶ࡾྜィࡍࡿ㸪 㐨┴࡛ 2008 ᖺᗘ᥇ྲྀࡉࢀࡓ EKC ࡢ᳨య 102 ௳୰ 11 ࢘ࣝࢫศ㞳ࡼࡾ 460 ௳୰ 165 ௳㸦35.9㸣㸧ࡽ ௳ࡽ᳨ฟࡉࢀ㸪ࡇࡢ࠺ࡕ 2 ௳ࡣᮏ┴࡛᥇ྲྀࡉࢀࡓ᳨ HAdV ࡀศ㞳ࡉࢀࡓࠋPCR ἲ࡛ࡣࡇࢀࡽࢆࡍ࡚ྵࡴ యࡽ᳨ฟࡉࢀ࡚࠸ࡿࠋ࡞࠾㸪ࡇࡢ 2 ௳ࡣᙜᡤ࡛ࡣ࢘ 192 ௳㸦41.7㸣㸧ࡽ HAdV ࡀ᳨ฟࡉࢀࡓࠋ࡞࠾㸪࢘ ࣝࢫࡀศ㞳࡛ࡁ࡞ࡗࡓࡓࡵ㸪ᅇࡢ᳨ᰝᡂ⦼ࡣ ࣝࢫศ㞳㝜ᛶ࣭PCR 㝧ᛶ࡞ࡗࡓ HAdV ࡢᆺࡣ㸪3 ྵࡲࢀ࡚࠸࡞࠸ࠋ௨ᚋ㸪2010 ᖺᗘࡽẖᖺᗘ᳨ฟࡉࢀ㸪 ᆺࡀ 1 ௳㸪8 ᆺࡀ 14 ௳㸪53 ᆺࡀ 2 ௳㸪54 ᆺࡀ 6 ௳㸪 ⌧ᅾ࡛ࡣ EKC ࡽ᳨ฟࡉࢀࡿせ࢘ࣝࢫࡢ୍ࡘ ᆺ᫂㸦NT㸧ࡀ 4 ௳࡛࠶ࡗࡓ㸦ࢹ࣮ࢱᮍᥖ㍕㸧ࠋ ࡞ࡗ࡚࠸ࡿࠋ ࡇࢀࡽ 3 ✀ࡢ᪂ᆺ HAdV ࡣ࣓࢟ࣛ࢘ࣝࢫ࡛࠶ࡾ㸪 㸧+$G9 ࡢᆺࡼࡿ⮫ᗋ≧ࡢ┦㐪 ୰ヨ㦂࠾࠸࡚㸪ከࡃࡢᰴ࡛ 8 ᆺᢠ⾑Ύࡢᕪ ཎయᐃⅬࡢ║⛉་㝔ࡽᚓࡽࢀࡓሗࡼࡿ㸪 ᪂ᆺ HAdV ࡼࡿ EKC ࡢ⮫ᗋᡤぢࡣ㸪ࡢᆺࡼࡿ ᛂࡀࡳࡽࢀ㸪ᚑ᮶ࡢ HAdV ᢠ⾑Ύࢆ⏝࠸ࡓ୰ᛂ࡛ EKC ࡼࡾ㔜࡞⤖⭷⾑ࢆ♧ࡍࡀከࡃ㸪ࡉࡽゅ⭷ ࡣᆺูỴᐃࡀᅔ㞴࡛࠶ࡿࡇࡀ♧ࡉࢀࡓࠋࡋࡓࡀࡗ࡚㸪 㞀ᐖࡶከ࠸ഴྥࡀㄆࡵࡽࢀࡿࠋࡲࡓ㸪⒵ࡲ࡛せࡍ ᚋࡣ㸪ࢤࣀ࣒ࡢ 3 㡿ᇦࡢ୍㒊ࢆቑᖜࡋ࡚ሷᇶ㓄ิࢆ ࡿᮇ㛫ࡣ㸪᪂ᆺཬࡧ 37 ᆺ࡛ 17㹼18 ᪥㛫㸪ࡑࡢࡢᆺ Ỵᐃࡍࡿ⡆᫆ᆺูἲࡸࣇࣝࢤࣀ࣒ゎᯒ࡛ᆺูࢆ⾜࠺ᚲ ࡛⣙ 12 ᪥㛫⛬ᗘ࡛࠶ࡗࡓࠋ せࡀ࠶ࡿࠋ ࡲࡓ,ศᏊ⣔⤫ᶞゎᯒࡢ⤖ᯝ㸪ྛᆺࡢ HAdV ࡣ㸪hexon㸪 ⪃ ᐹ fiber㸪penton ࡢ 3 㡿ᇦࡶ㸪ᆺࡈ᫂░࡞ࢡࣛࢫࢱ EKC ࡢཎᅉ࡞ࡿ HAdV ࡢᆺࡣ㸪E ✀ࡢ 4 ᆺ㸪B ✀ ࣮ࢆᙧᡂࡋࡓࠋࡉࡽ㸪Shimada ࡽ 10㸧ࡸ Hiroi ࡽ 17㸧 ࡢ 3 ᆺཬࡧ 11 ᆺ➼ࢆ㝖ࡃ㸪 D ✀࡛࠶ࡾ㸪ࡇࢀ ࡢศᯒ⤖ᯝྠᵝ㸪ྛࢡࣛࢫࢱ࣮ෆ࡛ࡣᶆ‽ᰴྛᰴ ࡲ࡛ᅜⓗ 8 ᆺ㸪19 ᆺ㸪37 ᆺ࡞ࡀὶ⾜ࢆ⧞ࡾ㏉ࡋ ࡀ㠀ᖖ㧗࠸┦ྠᛶࢆ♧ࡋࡓࠋࡋࡋ࡞ࡀࡽ㸪ศ㞳ᖺ ࡚ࡁࡓࠋᅇ㸪2008 ᖺᗘࡽ 2013 ᖺᗘࡲ࡛㸪║⛉ ᗘࡼࡿࢃࡎ࡞㐪࠸࡛ࢧࣈࢡࣛࢫࢱ࣮ࢆᙧᡂࡋࡓᰴ ࡢ⮫ᗋ᳨యࡽ᳨ฟࡉࢀࡓ HAdV ࡢศᏊᏛゎᯒࢆ⾜ ࡶࡳࡽࢀࡓࡇࡽ㸪HAdV ࡣྛ㡿ᇦࡢሷᇶ㓄ิࢆಖ ࡗࡓࡇࢁ㸪12 ࡢᆺࡀ᳨ฟࡉࢀ㸪EKC デ᩿ࡉࢀࡓ⮫ ࡕ࡞ࡀࡽࡶࢃࡎኚ␗ࡋ㸪ὶ⾜ࢆ⧞ࡾ㏉ࡋ࡚࠸ࡿྍ ᗋ᳨యࡽ 3 ᆺ㸪4 ᆺ㸪8 ᆺ㸪37 ᆺ㸪53 ᆺ㸪54 ᆺ㸪ཬ ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ HAdV ࡢ᳨ᰝἲ㛵ࡋ࡚㸪Ლཎࡽ ࡧ 56 ᆺࡀከᩘ᳨ฟࡉࢀࡓࠋ≉㸪4 ᆺཬࡧ 8 ᆺࡣ㸪㏆ 㸪8 ᆺࡣ 2013 ᖺᗘᛴቑࡋࡓࠋFujimoto ࡽ ࡣ࢘ࣝࢫศ㞳 ࡛ࡣ 30.4㸣࡛࠶ࡗࡓ㝧ᛶ⋡ࡀ㸪PCR ἲ࡛ࡣ 73.9㸣㸪 ᖺࢇ᳨ฟࡉࢀ࡚࠸࡞ࡗࡓࡀ㸪4 ᆺࡣ 2012 ᖺᗘ 15㸧 18㸧 ⣙ 2.4 ಸ㧗ឤᗘ࡞ࡗࡓሗ࿌ࡋ࡚࠸ࡿࠋᅇ㸪ᡃࠎ ࡶ㸪 ࡢㄪᰝ࡛ࡣ㸪࢘ࣝࢫศ㞳ࡢࡳ⾜ࡗࡓ 2008 ᖺᗘ 2009 4) Walsh Michael P, Chintakuntlawar Ashish, Robinson ᖺࡢ㝧ᛶ⋡ࡣ 30㸣⛬ᗘ࡛࠶ࡗࡓࡀࠊPCR ἲࢆే⏝ࡋࡓ Christopher M, Madisch Ijad, Harrach, Bala´ zs, Hudson 2010 ᖺᗘ௨㝆ࡣྛᖺᗘ 40㸣௨ୖ࡞ࡾ㸪ᗄศ᳨ฟ⋡ࡀ Nolan R., Schnurr David, Heim Albert, James Chodosh, ྥୖࡋࡓࠋ࢘ࣝࢫࢆศ㞳ࡋಖᏑࡋ࡚࠾ࡃࡇࡣ㸪ᑗ Seto Donald, Jones Morris S㸸 PLoS ONE 㸦2009㸧㸬 ᮶ࡢヲ⣽࡞ゎᯒࡢࡓࡵᚲせྍḞ࡛࠶ࡿࡀ㸪࢘ࣝ 5) Ishiko H, Shimada Y, Konno T, Hayashi A, Ohguchi T, Tagawa ࢫࡀศ㞳ࡉࢀࡓ᳨యࡣࡍ࡚ PCR ἲ᳨࡛ฟࡉࢀࡓࡇ Y, Aoki K, Ohno S, Yamazaki S. J Clin Microbiol,46(6):2002-8 ࡽ㸪ὶ⾜≧ἣࢆ㎿㏿ㄪᰝࡍࡿࡓࡵࡣ㸪㛫 (2008) 㸬 6) Kaneko H, Suzutani T, Aoki K, Kitaichi N, Ishida S, ᡭ㛫ࡢࡿ࢘ࣝࢫศ㞳ࡼࡾ㸪PCR ἲࡢ᪉ࡀ⡆౽࡛ ຠ⋡ⓗ࡞᪉ἲ࡛࠶ࡿࡇࡀ♧ࡉࢀࡓࠋ Ishiko H, Ohashi T, Okamoto S, Nakagawa H, ࡲࡓ㸪ᆺࡼࡿ⮫ᗋ≧ࡢ┦㐪ࢆㄪᰝࡋࡓࡇࢁ㸪 Hinokuma R, Asato Y, Oniki S, Hashimoto T, Iida T, ᪂ᆺࡼࡿ EKC ࡣ≧ࡢ㔜㸪⒵ᮇ㛫ࡢᘏ㛗ࡀㄆ Ohno S㸸Br J Ophthalmol 95, 32–36 (2011)㸬 ࡵࡽࢀࡓࡀ㸪ࡇࢀࡣ㸪᪂ᆺ HAdV ࡀ㏆ᖺὶ⾜ࡋጞࡵࡓ 7) Robinson CM, Singh G, Henquell C, Walsh MP, ࡇࡼࡾ㸪᪂ᆺ HAdV ࡢᢠయࡢಖ᭷⪅ࡀᑡ࡞࠸ࡇ Peigue-Lafeuille H, Seto D, Jones MS, Dyer DW, ࡶ୍ᅉ࡛࠶ࡿ᥎ᐹࡉࢀࡓࠋ Chodosh J.㸸Virology. 20,409(2),141-7㸦2011㸧㸬 ௨ୖࡢࡇࡽ㸪ࢃࡎ࡞ࡀࡽࡶ㑇ఏᏊࡢኚ␗ࡸ㑇 8) Kaneko H, Aok K, Ohno S, Ishiko H, Fujimoto T, ఏᏊࡢ⤌ࡳኚࢃࡗࡓ᪂ᆺ HAdV ࡢὶ⾜➼ࡀㄆࡵࡽࢀࡿ Kikuchi M, Harada S, Gonzalez G, Koyanagi K O, ࡇࡽ㸪ᚋࡶᘬࡁ⥆ࡁㄪᰝࢆ⥅⥆ࡍࡿࡇࡀ㔜せ Watanabe H, Suzutani1 T㸸J Clin Microbiol.49, 484–490 ࡛࠶ࡿࠋ 㸦2011㸧㸬 9) Saitoh-Inagawa W, Oshima A, Aoki K, Itoh N, Isobe K, Uchio E, Ohno S, Nakajima H, Hata K, Ishiko H: J Clin ࡲࡵ Microbiol.䠈, 2113-2116. 䠄1996䠅䠊 ║⛉ᐃⅬ࡛ EKC ࡽ᳨ฟࡉࢀࡿ HAdV ࡢศᏊ Ꮫᆺゎᯒࢆ⾜ࡗࡓࡇࢁ㸪ᮏ┴࡛ࡶὶ⾜ᆺࡢᖺḟኚ 10) Shimada Y, Ariga T, Tagawa Y, Aoki K, Ohno S, Ishiko H㸸J Clin Microbiol.42(4),1577-1584㸦2004㸧㸬 ࡀࡳࡽࢀ㸪4 ᆺ㸪8 ᆺ㸪37 ᆺ㸪ཬࡧ 53 ᆺ࡛ὶ⾜ᖺᗘ ࡀ☜ㄆࡉࢀࡓࠋ2008 ᖺᗘࡽ㸪᪂ᆺ HAdV ࡀ᳨ฟࡉࢀ 11) Banik U, Adhikary AK, Suzuki E, Inada T, Okabe N.㸸J Clin Microbiol.43(3):1064-8㸦2005㸧㸬 ࡣࡌࡵ㸪ࡇࢀࡽࡢᆺࡼࡿ EKC ࡣᚑ᮶ࡢᆺࡼࡾ㔜࡛ 12) ᅜ❧ឤᰁ◊✲ᡤ⦅㸸ཎయ᳨ฟ࣐ࢽࣗࣝ㸦ဗ ⒪ࡀ㛗ᘬࡃഴྥࡀㄆࡵࡽࢀࡓࠋ 㢌⤖⭷⇕࣭ὶ⾜ᛶゅ⤖⭷⅖᳨ᰝ㸪デ᩿࣐ࢽࣗࣝ㸪 ࡲࡓ㸪᪂ᆺ HAdV ࡛ࡣ㸪ᕪᛂࡼࡾᚑ᮶ࡢ HAdV ➨ 2 ∧㸧(2012)㸬 ᢠ⾑Ύࢆ⏝࠸ࡓ୰ヨ㦂ࡀᅔ㞴࡛࠶ࡿࡇࡽ㸪hexon㸪 13) Xu W㸪McDonough MC㸪 Erdman DD㸸 J. 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