Cell isolation and digital characterization by BD Flow Cytometry and

日本生化学会大会
バイオインダストリーセミナー
第87回
共催：第87回日本生化学会大会／日本ベクトン・ディッキンソン株式会社／アズワン株式会社
日時
会場
2014 年 10月 17日（金）11:45∼12:35
第12会場（国立京都国際会館 2階 Room B-1）
Cell isolation and digital characterization by BD Flow
Cytometry and single cell gene expression profiling
using the NanoString nCounter® Analysis System
Chair/Speaker:
Dr. J. Clark Mason
Senior Director of Global Strategic Planning, BD Biosciences
Speaker: Michael
Rhodes
Director of Collaborations and Applications, NanoString Technologies, Inc.
Recent advances in the sensitivity of next generation sequencing (NGS)
and gene expression technologies have enabled the analysis of complex
cell micro environments. However, there remains the challenge to
separate and identity homogeneous cell populations prior to analysis.
Here we describe how the power of flow cytometry can be exploited to
isolate, characterize, and purify single cell populations from complex cell
populations, prior to gene expression profiling. We describe a complete
workflow starting with heterogeneous cancer cells that can be isolated at
rapid speed by single cell depositions into 96 well plates using the newly
developed flow cytometer from BD Biosciences, the BD FACSJazz™. We
demonstrate the use of new analytical tools such as index cell sorting
that can help track those individual cells during and after sorting. Finally
we demonstrate that the single cell populations are of high fidelity and
suitable for gene expression analysis using the NanoString nCounter®
Analysis System with gene expression assay panels capable of
simultaneously measuring up to 800 gene expression targets.
日本ベクトン・ディッキンソン株式会社
カスタマーサービス
0120-8555-90
www.bd.com/jp/
＊nCounter®はNanoString Technologies, Inc.の登録商標です。
＊BD、BDロゴおよびBD FACSJazzの商標はBecton, Dickinson and Companyが保有します。©2014 BD
バイオサイエンスグループ ☎06-6447-8633
www.bio-lab.jp

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