活性部位反応型ＤＵＢプローブ 脱ユビキチン化酵素（DUB)用の活性部位反応型プローブは、多くのDUBの活性部位に存在するシス テイン残基に反応するよう設計されており、ユビキチンのC末端にDUB阻害剤であるVME（vinylmethyl ester）またはPA（propagylamide）が結合しています。（Figure 1） UbiQ社ではVME（vinylmethyl ester）とPA（propagylamide）の2種類の反応基と、各反応基 で4種類のN末端タグ（TAMRA、Cy5、biotin、HA---＞Table 3）を付加したプローブ(Table 1) をご用意しております。 Figure 1 western blotting, affinity tag direct in-gel read-out HA Biotin TAMRA Cy5 Ub HA Biotin TAMRA Cy5 Ub VME DUB PA DUB Table 1 - our range of DUB activity probes VME1 PA2 tag code name code name − UbiQ-005 Ub-VME UbiQ-057 Ub-PA HA UbiQ-035 HA-Ahx-Ahx-Ub-VME UbiQ-078 HA-Ahx-Ahx-Ub-PA Biotin UbiQ-054 Biotin-Ahx-Ub-VME UbiQ-076 Biotin -Ahx -Ub-PA TAMRA UbiQ-050 TAMRA-Ub-VME UbiQ-058 TAMRA-Ub-PA Cy5 UbiQ-071 Cy5-Ub-VME UbiQ-072 Cy5-Ub-PA UbiQ-L02 DUB probe explorer panel (our full range of all 10 DUB activity probes) Table 2 – specifications of VME and PA warhead VME PA targets USP and UCH DUBs targets OTU, USP and UCH DUBs * irreversible DUB inhibitor irreversible DUB inhibitor which forms a covalent linkage that can be cleaved by acid treatment * According to a proteomics study the PA probes also target DUBs of the Machado-Josephin Domain family. 2 Table 3 – specifications of N-terminal tags TAMRA1a Cy51a Biotin1 HA1 fluorescence detection fluorescence detection affinity tag affinity tag • fast (in-gel) -, sensitive -, • fast (in-gel) -, sensitive -, • allows pull-down with and distinct read-out. • no background labelling by cross reactivity as seen in immunoblots • exc 625 nm, abs 670 nm • allows pull-down with HA biotin binding tools binding tools • strongest known non-co- • influenza epitope tag valent interaction • detection by western-blot• detection by western-blotting ting fact sheet, October 2014 and distinct read-out. • no background labelling by cross reactivity as seen in immunoblots • exc 550 nm, abs 590 nm literature 1(a) de Jong et al. ChemBioChem 2012, 13, 2251. (b) Altun et al. Chem Biol 2011, 18, 1401. 2 Ekkebus et al. J Am Chem Soc 2013, 135, 2867. (b) Sommer et al. Bioorg Med Chem 2013, 21, 2511. for a complete list of references we refer to the product group overview document. Science Park 408, 1098 XH Amsterdam, The Netherlands [email protected] +31 20 303 1970 www.ubiqbio.com
© Copyright 2024 ExpyDoc
