ᐇ㦂␒ྕ㸸 2014PA005 2014 ᖺᗘ๓ᮇ ᡂᯝබ㛤↓ൾ⏝ᴗ ᡂᯝሗ࿌᭩ ‒ ⇟⇓∙⇗⇈ဇ⇶⇊⇐⇛∞⇻⇇⇕⇥∙⇮ ‿‷‾ ↝ನᡯ↗‒ ↺ↈ᧙↚ע࣎↹رᄂᆮ‒ Study of biosurfactant MEL structure in skincare products and the effects on the application feelings. ⯪ᇛ ୍㸪ᑠᯇ 㝧Ꮚ㸪ᒣᮏ ࿘ᖹ㸪⃝ ᏹ᫂ Kenichi Funaki , Yoko Komatsu, Shuhei, Yamamoto, Hiroaki Kitazawa ᮾὒ⣳ᰴᘧ♫ TOYOBO Co., LTD. 1. ᐃᐇ᪥ 2014 ᖺ 6 ᭶ 19 ᪥ 2014 ᖺ 6 ᭶ 19 ᪥ 2014 ᖺ 9 ᭶ 2 ᪥ 2014 ᖺ 9 ᭶ 17 ᪥ 10 – 14 00 ศ 14 30 ศ – 18 30 ศ 10 – 18 30 ศ 10 – 18 30 ศ (1 ࢩࣇࢺ) (1 ࢩࣇࢺ) (2 ࢩࣇࢺ) (2 ࢩࣇࢺ) 㸪BL6N1 㸪BL8S3 㸪BL8S3 㸪BL8S3 2. ᴫせ ࣂ࢜ࢧ࣮ࣇࢡࢱࣥࢺ㸪࣐ࣥࣀࢩ࢚ࣝࣜࢫࣜࢺ࣮ࣝࣜࣆࢵࢻ(MEL)ࡢྛ✀ ⢝ရཎᩱඹᏑୗ࡛ࡢᵓ㐀ࢆᑠゅᩓἲࡼࡾ᫂ࡽࡋ㸪⢝ရࡋ࡚ࡢሬ ࡾᚰᆅࡢᐁ⬟ホ౯⤖ᯝࡢ┦㛵ࢆㄪࡿࡓࡵࡢᇶ♏ࢹ࣮ࢱࢆྲྀᚓࡋࡓࠋ 㸬⫼ᬒ◊✲┠ⓗ ㏆ᖺ㸪᭷㝈࡞▼Ἔ㈨※ࡽ⏕ྍ⬟࡞ࣂ࣐࢜ࢫ㈨※ࡢཎᮦᩱ㌿ࡀồࡵ ࡽࢀ࡚࠾ࡾ㸪⢝ရ࠾࠸࡚ࡶࣄࢺࡔࡅ࡛࡞ࡃᆅ⌫ࡶඃࡋ࠸⢝ရ Sustainable Cosmetics ࡀὀ┠ࡉࢀࡘࡘ࠶ࡿࠋ⢝ရࡣ⏺㠃άᛶࡀᚲせྍ Ḟ࡞ᡂศ࡛࠶ࡿࡀ㸪ᡃࠎࡣ⎔ቃㄪࡋࡓ⏺㠃άᛶࡋ࡚ᚤ⏕≀ࡼࡗ࡚ࣂ ࣐࢜ࢫ㈨※ࡽ㔞⏘ࡉࢀࡿࣂ࢜ࢧ࣮ࣇࢡࢱࣥࢺ(BS㸧╔┠ࡋࡓࠋࣂ࢜ ࢧ࣮ࣇࢡࢱࣥࢺࡢྜᡂࡣࡍ࡚㓝⣲ᛂࡼࡗ࡚⨨㑅ᢥⓗ࣭❧య㑅ᢥⓗ ⾜ࢃࢀࡿࡓࡵศᏊࡢྥࡁ࣭ᙧࡀࡑࢁࡗ࡚࠸ࡿࡓࡵ⏺㠃࡛ຠ⋡ⓗ࡞ศᏊ㞟ྜࡸ㓄 ྥࡀྍ⬟࡞ࡿࡓࡵ᪤Ꮡࡢྜᡂ⏺㠃άᛶẚ࡚ࡼࡾప⃰ᗘ࡛ᶵ⬟ࢆⓎ࡛ ࡁࡿࠋ ⌧ᅾࣂ࢜ࢧ࣮ࣇࢡࢱࣥࢺࡣᩘ༑✀㢮ࡀ▱ࡽࢀ࡚࠸ࡿࡀ㸪ࡑࡢ୰࡛ࡶ࠶ࡿ ✀ࡢ㓝ẕࡼࡗ࡚⏕⏘ࡉࢀࡿ⢾ᆺ BS ࡢ୍✀࡛࠶ࡿ࣐ࣥࣀࢩ࢚ࣝࣜࢫࣜࢺ࣮ ࣝࣜࣆࢵࢻ(MEL) ὀ┠ࡋࡓࠋMEL ࡣ⏕⏘㓝 ẕࡼࡾ ᵓ㐀ⓗ࡞ከᵝᛶ (MEL-A, B, C)ࡀᏑᅾࡍࡿࡀ㸪ࡑࡢ୰࡛ ࡶ⟃Ἴᒣࡢⰼࡽ༢㞳ࡉࢀࡓ㓝ẕ P. tsukubaensis ࡀ⏘ฟࡍࡿ MEL-B(Fig. 1)ࡢඃࢀࡓ⏺㠃άᛶ⬟㸪࣓ࣛࣛᙧᡂ⬟ Figure1. MEL-B ࡢศᏊᵓ㐀ࠋAc ࡣࢭࢳ ࣝᇶ,n=8~12 ࢆ⏕ࡋࡓ〇ရ㛤Ⓨࢆ⾜ࡗ࡚࠸ࡿࠋ MEL-B ࡣ௦⾲ⓗ࡞ゅᒙ⣽⬊㛫⬡㉁࡛࠶ࡿኳ↛ࢭ࣑ࣛࢻ㢮ఝࡋࡓᵓ㐀ࢆᣢ ࡗ࡚࠾ࡾ㸪ࢭ࣑ࣛࢻྠᵝࡢಖ‵ຠᯝࢆ᭷ࡍࡿࡇࡀ▱ࡽࢀ࡚࠸ࡿࠋࡲࡓ⫙ሬ ᕸࡋࡓሙྜඃࢀࡓ⫙Ⲩࢀᨵၿຠᯝࢆᣢࡘࡇࡀࣄࢺ 3 ḟඖᇵ㣴⓶⣽⬊ࢆ⏝࠸ ࡓࣔࢹࣝᐇ㦂ࡽ᫂ࡽ࡞ࡗࡓࠋࡇ࠺ࡋࡓຠᯝࡣ MEL-B ࡀ᭷ࡍࡿ⏺㠃άᛶࡔ ࡅ࡛࡞ࡃࠊඃࢀࡓ࣓ࣛࣛᙧᡂ⬟ࢆ᭷ࡍࡿࡇࡽゅᒙࡢ⣽⬊㛫ᾐ㏱ࡋࠊ࣓ࣛ ࣛᒙࢆᙧᡂࡍࡿࡇ࡛⓶ࡽࡢỈศᩓࢆ㜵ࡄࡇࡼࡿࡶࡢ⪃࠼ࡽࢀ࡚ ࠸ࡿ 1)ࠋ ࡇ࠺ࡋࡓ MEL ࡢ⢝ရཎᩱࡋ࡚ࡢຠ⬟ࢆ◊✲ࡍࡿ୰࡛㸪ᐁ⬟ホ౯ࡼࡾ㸪 ⢝ရ MEL ࢆᑡ㔞ῧຍࡍࡿ㸪ሬᕸᚋࡢࠕࡓࡘࡁឤࠖࢆᨵၿࡍࡿຠᯝࡀ࠶ ࡿࡇࡀ☜ㄆࡉࢀࡓࠋ ࠕࡓࡘࡁឤࠖࡢࡁ࡞⢝ရࡣᾘ㈝⪅᎘ࢃࢀࡿഴྥࡀ ࠶ࡿࡓࡵ㸪ࠕࡓࡘࡁឤࠖࡢᨵၿ㛵ࡍࡿ◊✲ 2㸧ࡀሗ࿌ࡉࢀ࡚࠸ࡿࠋࡑࡢࡓࡵ㸪 MEL ῧຍࡼࡿࠕࡓࡘࡁឤࠖᨵၿຠᯝࡢ࣓࢝ࢽࢬ࣒ࡢ᳨ドࡣ㸪㔜せ࡞◊✲㛤 Ⓨࢸ࣮࣐࡛࠶ࡿ⪃࠼࡚࠸ࡿࠋࡉࡽ⣲ᮦࡢᵓ㐀⓶ᑐࡍࡿሬࡾᚰᆅࡢ࣓ ࢝ࢽࢬ࣒ࢆ᫂ࡽࡍࡿࡇ࡛ࢫ࢟ࣥࢣ⏝㏵ࡢࡳ࡞ࡽࡎ⢝ရ⣲ᮦ࣭ሬࡾ ⸆࡞ࡢᒎ㛤ࡶᮇᚅ࡛ࡁ㸪⏕άࡢ㉁㸦QOL㸧ࡢྥୖᐤ࡛ࡁࡿᮇᚅࡋ࡚ ࠸ࡿࠋ ࡇ࠺ࡋࡓ⫼ᬒࡢࡶᮏ◊✲࡛ࡣ㸪MEL ࡢ⓶ᑐࡍࡿሬࡾᚰᆅࡢ࣓࢝ࢽࢬ࣒ ࢆゎ᫂ࡍࡿࡇࢆ┠ⓗࡋ㸪ࡑࡢᇶ♏᳨ウࡋ࡚ྛ✀⢝ရཎᩱࡢ┦≧ែ ࡸᒁᡤᵓ㐀ࢆᨺᑕගࢆ⏝࠸࡚ㄪࡓࠋ 㸬ᐇ㦂ෆᐜ ᐃࡣ BL8S3 ࣅ࣮࣒࡛ࣛࣥᐇࡋࡓࠋ ヨᩱ᳨ฟჾ㛫㊥㞳ࡣ⣙ 1.1 m㸪X ⥺ Ἴ 㛗 ࡣ 0.92 ύ ࡋ 㸪 ᳨ ฟ ჾ ࡣ RIGAKU 〇 ࣓ ࣮ ࢪ ࣥ ࢢ ࣉ ࣞ ࣮ ࢺ R-AXISIV++ࢆ⏝࠸ࡓࠋヨᩱࡋ࡚ࡣ⢝ရཎᩱࡋ࡚⏝࠸ࡽࢀࡿ 1,3-ࣈࢳࣞ ࣥࢢࣜࢥ࣮ࣝ(BG)㸪ࢢࣜࢭ࣮ࣟࣝ (GLY)㸪MEL㸪Ỉࢆ⾲ 1 ࡢྜ࡛ΰ ྜࡋࡓヨᩱࢆ ᐃヨᩱࡋࡓࠋヨᩱ 1㹼5 ࡣ⢓ᛶࡀ㧗࠸ࡓࡵࢫࢸࣥࣞࢫ 〇 1.6 mm ཌࡢࢫ࣮࣌ࢧ࣮୧ഃ 7.5 Pm ཌࡢ࢝ࣉࢺࣥࣇ࣒ࣝࢆ㈞ ࡾࡑࡢ୰ヨᩱࢆᑒධࡋ࡚ࣅ࣮࣒ Table 1. ᐃ⏝࠸ࡓヨᩱ ヨᩱ 㻝 㻞 㻟 㻠 㻡 㻢 㻣 㻤 ⤌ᡂ 㻹㻱㻸㻛Ỉ㻌㻩㻌㻣㻜㻛㻟㻜㻌㼣㼠㻑 ヨᩱ㻝䛻㻮㻳㻞㻜㼣㼠㻑ΰྜ ヨᩱ㻝䛻㻮㻳㻠㻜㼣㼠㻑ΰྜ ヨᩱ㻝䛻㻳㻸㼅㻞㻜㼣㼠㻑ΰྜ ヨᩱ㻝䛻㻳㻸㼅㻠㻜㼣㼠㻑ΰྜ 㻹㻱㻸㻛㻮㻳㻛Ỉ㻌㻩㻌㻤㻜㻛㻝㻜㻛㻝㻜㻌㼣㼠㻑 ヨᩱ㻢䜢㻝㻜ಸᕼ㔘 ヨᩱ㻢䜢㻝㻜㻜ಸᕼ㔘 ࣛࣥࡢ࣮࢜ࢺࢧࣥࣉ࣮ࣛᅛᐃࡋ࡚ ᐃࡋ㸪ヨᩱ 6~8 ࡣ≉ὀࡋࡓ⁐ᾮࢭࣝ ࢆࣅ࣮࣒ࣛࣥᅛᐃࡋ࡚ ᐃࡋࡓࠋ⏝࠸ࡓ⁐ᾮࢭࣝࡣὶ㊰ࡢ୧ഃࢺࢵࢡ ♫〇ࡢᴟⷧ 10 Pm ࡢ▼ⱥ࢞ࣛࢫࢆ⏝࠸ X ⥺ࡢῶ⾶ࢆ㜵ࡂ㸪ࢭࣝࢆᅛᐃࡋࡓࡲ ࡲヨᩱᾮయࡢኚ᭦ࡀྍ⬟࡞タィ࡛࠶ࡿࡓࡵప⃰ᗘࡢ⁐ᾮ࡛ࡶ⁐፹ࡽࡢᩓ ࢆ㧗⢭ᗘ㝖ཤࡍࡿࡇࡀྍ⬟࡞ࡗ࡚࠸ࡿࠋ 㸬⤖ᯝ࠾ࡼࡧ⪃ᐹ ᚓࡽࢀࡓ࣓࣮ࢪࣥࢢࣉ࣮ࣞࢺീࢆ⎔ᖹᆒࡋ 1 ḟඖࡋࡓࣉࣟࣇࣝ ࢆ Fig.2~4 ♧ࡋࡓࠋࡇࡇ࡛ᶓ㍈ࡣࡍ࡚ᩓ࣋ࢡࢺࣝ s (=(2 /Ȣ)sinȟ:Ȣࡣ X ⥺Ἴ㛗,ȟࡣᩓゅ)ࡋࡓࠋヨᩱ 1 ࡢ MEL/Ỉ=70/30 wt%⣔࡛ࡣ MEL ࡣ㠃 㛫㝸 d=4.4 nm ࡢᆺⓗ࡞࣓ࣛࣛᵓ㐀ࢆࡗ࡚࠸ࡿࡇࡀࢃࡿࠋࡇࡢ⣔ 1,3-ࣈࢳࣞࣥࢢࣜࢥ࣮ࣝ㸪ࢢࣜࢭ࣮ࣟࣝࢆ 20 wt%, 40 wt%ῧຍࡋࡓ⣔ࡽࡢ ᩓࣉࣟࣇࣝࡣࡑࢀࡒࢀ Fig.2, Fig.3 ࡢࡼ࠺ኚࡋࡓࠋࢢࣜࢭ࣮ࣟࣝࢆ ῧຍࡋࡓሙྜࡣ࣓ࣛࣛᵓ㐀ࢆಖࡗࡓࡲࡲ MEL ࡢ㠃㛫㝸ࡀᗈࡀࡿࡢᑐࡋ࡚㸪 1,3-ࣈࢳࣞࣥࢢࣜࢥ࣮ࣝࢆῧຍࡋࡓሙྜ࣓ࣛࣛᵓ㐀ࡀᔂࢀࡿࡇࡀࢃࡗࡓࠋ ู㏵ᐇࡋࡓᐁ⬟ホ౯ࡢ⤖ᯝ㸪MEL ࢆᑡ㔞ῧຍࡋࡓሙྜ 1,3-ࣈࢳࣞࣥࢢࣜࢥ ࣮ࣝ⣔࡛ࡣࠕࡓࡘࡁឤࠖࡀᨵၿࡉࢀࡿࡀࢢࣜࢭ࣮ࣟࣝ⣔ῧຍࡋ࡚ࡶࠕࡓ ࡘࡁឤࠖࡣᨵၿࡉࢀ࡞࠸ࡇࡀ♧ࡉࢀ࡚࠾ࡾ㸪࣓ࣛࣛᵓ㐀ࡢᔂࢀࡀ MEL ࡢࠕ ࡓࡘࡁឤࠖᨵၿຠᯝ㛵ಀࡋ࡚࠸ࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ௨ୖࡣ MEL ⃰ཌ⁐ ᾮ࡛ࡢ⤖ᯝ࡛࠶ࡿࡀ㸪ᐇ㝿ࡢ⢝ရ୰࡛⏝࠸ࡽࢀࡿ MEL ࡢ⃰ᗘࡣ 0.1 wt%⛬ ᗘ࡛࠶ࡾ㸪ࡇࡢࡼ࠺࡞ప⃰ᗘ㡿ᇦ࡛ MEL ࡀࡢࡼ࠺࡞ᵓ㐀ࢆࡿࡢࢆㄪ ࡓ⤖ᯝࢆ Fig.4 ♧ࡋࡓࠋヨᩱ 6(MEL/BG/Ỉ= 80/ 10/ 10 wt%)࡛ࡣ MEL ࡣ ࣓ࣛࣛᵓ㐀ࡣࡽࡎࢫ࣏ࣥࢪᒙ(L3)㏆ᵓ㐀ࢆࡗ࡚࠸ࡿࡶࡢ⪃࠼ࡽࢀࡿ 3)ࡀ㸪Ỉ࡛ 10 ಸᕼ㔘㸪100 ಸᕼ㔘ࢆ⾜࠸ ᐃࡋࡓࡇࢁ࣓ࣛࣛᵓ㐀ࡀฟ⌧ࡍ ࡿࡇࡀࢃࡗࡓࠋᐇ㦂࡛ࡣ㟢ග㛫ࡣ 900 ⛊ࡋࡓࡀ㸪0.1 wt%⛬ᗘ࡛ࡶ༑ ศᵓ㐀ࡀゎᯒ࡛ࡁࡿ⛬ᗘࡢࢫ࣌ࢡࢺࣝࡀᚓࡽࢀࡿࡇࡀࢃࡗࡓࠋ ࡇࡢࡼ࠺ MEL ࡣỈࡸࡢ⢝ရཎᩱ┦స⏝ࡋ」㞧࡞ᵓ㐀┦㌿⛣ࢆ ⏕ࡌ㸪ᕼⷧ⁐ᾮ୰࡛ࡶ࣓ࣛࣛᙧᡂ⬟ຊࡀ㧗࠸ࡇࡀ☜ㄆ࡛ࡁࡓࠋࡇ࠺ࡋࡓ▱ぢ ࢆඖ┦ᅗࢆヲ⣽ㄪ⓶ᾐ㏱ࡍࡿ๓ᚋ࡛ᵓ㐀ࢆไᚚࡍࡿࡇࡀ࡛ࡁࢀ ࡤᾐ㏱ࡋࡸࡍࡃࡘຠ⬟ࡢ㧗࠸〇ရࡢ㛤Ⓨࡘ࡞ࡀࡿᮇᚅࡋ࡚࠸ࡿࠋ 䢶 䢳䢲 䢳䢲 ヨᩱ䢳䢪䣏䣇䣎䢱Ỉ 䢿䢢䢹䢲䢱䢵䢲䢢䣹䣶䢧䢫 ヨᩱ䢴䢪ヨᩱ䢳僑䣄䣉䢴䢲䣹䣶䢧ΰྜ䢫 ヨᩱ䢵䢪ヨᩱ䢳僑䣄䣉䢶䢲䣹䣶䢧ΰྜ䢫 䢹 䢸 䢷 䢶 䢹 䢸 䢷 ヨᩱ䢳䢪䣏䣇䣎䢱Ỉ 䢿䢢䢹䢲䢱䢵䢲䢢䣹䣶䢧䢫 ヨᩱ䢶䢪ヨᩱ䢳僑䣉䣎䣛䢴䢲䣹䣶䢧ΰྜ䢫 ヨᩱ䢷䢪ヨᩱ䢳僑䣉䣎䣛䢶䢲䣹䣶䢧ΰྜ䢫 䢶 䢵 ヨᩱ䢸䢪䣏䣇䣎䢱Ỉ䢱䣄䣉䢢䢿䢢䢺䢲䢱䢳䢲䢱䢳䢲䢢䣹䣶䢧䢫 ヨᩱ䢹䢪ヨᩱ䢸僸Ỉ働䢳䢲ಸᕼ㔘䢫 ヨᩱ䢺䢪ヨᩱ䢸僸Ỉ働䢳䢲䢲ಸᕼ㔘䢫 䢵 䢳䢲 䢵 䢴 䢳䢲 䢹 䢸 䢷 䢶 䢵 䢳䢲 Intensity(Log, shited) 䢴 䢵 Intensity(Log) Intensity(Log) 䢶 䢵 䢹 䢸 䢷 䢶 䢵 䢴 䢳 䢴 䢴 䢳䢲 䢳䢲 䢹 䢴 䢳䢲 䢳䢲 䢴 䢹 䢲䢰䢴 䢲䢰䢶 䢲䢰䢸 䢲䢰䢺 䢲䢰䢴 s(nm-1) Figure. 2 MEL/Ỉ⣔ BG ࢆ ῧຍࡋࡓࡁࡢᩓࣉࣟࣇ ࣝࡢኚ 䢲䢰䢶 䢲䢰䢸 䢲䢰䢺 䢲䢰䢴 Figure 3. MEL/Ỉ⣔ GLY ࢆῧຍࡋࡓࡁࡢᩓࣉࣟ ࣇࣝࡢኚ 䢲䢰䢶 䢲䢰䢸 䢲䢰䢺 s(nm-1 ) s(nm-1) Figure 4.MEL/BG/Ỉࡢᕼ㔘 ࡼࡿᩓࣉࣟࣇࣝࡢ ኚ(ᙉᗘࢩࣇࢺ᭷) ࡞࠾ BL6N1 ࡛ࡢ XAFS ᐃࡘ࠸࡚ࡣ MEL Ỉ⁐ᾮ୰ᚤ㔞ྵࡲࢀࡿ≉ᐃ ࡢඖ⣲ࡀࡑࡢᏛ⤖ྜ≧ែࡼࡗ࡚ MEL Ỉ⁐ᾮࡢᛶ㉁ࢆᕥྑࡍࡿ௬ㄝࡢ ࡶ࡛ᐇࡋࡓࠋ࣒࣊ࣜ࢘㞺ᅖẼୗ࡛ࡢ⁐ᾮࡢ ᐃ࡛࠶ࡗࡓࡀ 100 ppm ௨ୗ ࡢప⃰ᗘ࡛ࡶ ᐃࡣ༑ศྍ⬟࡛࠶ࡗࡓࠋࣅ࣮࣒ࣛࣥࡢᛶ⬟ࡣ☜ㄆ࡛ࡁࡓࡀ ゎᯒࡋࡓ⤖ᯝ㸪Ꮫ⤖ྜ≧ែ᭷ព࡞ᕪࡀ࡞࠸ࡇࡀศࡗࡓࡢ࡛ࡑࢀ௨ୖ⥅ ⥆ࡋ࡞࠸ࡇࡋࡓࠋ ᚋࡢㄢ㢟 ๓ᮇࡢᐇ㦂࡛ᚓࡽࢀࡓ⤖ᯝࢆࡶ㸪⓶୰࡛ࡢ MEL ࡢᵓ㐀㸪MEL ࡢ⓶ ᵓ㐀࠼ࡿᙳ㡪㸪࠾ࡼࡧ⓶ᑐࡍࡿሬࡾᚰᆅࡢ┦㛵ᛶࢆㄪࡿࡓࡵ㸪 ⓶ࡶࡋࡃࡣேᕤ⓶ࡢᾐ㏱๓ᚋ࡛ࡢ⓶ᵓ㐀ࡢኚࡘ࠸࡚ほᐹࡍࡿࡇ ࢆᚋᮇᐇࡋࡓ࠸ࠋ ཧ⪃ᩥ⊩ 1) ᒣᮏ ࿘ᖹ, ᰗ㇂ ࿘స, ᳃⏣ ᓅ 㸪⾲㠃 50(10), 351-362, (2012) 2) ᕤ⸨ᶞᰗᏹ୍ྜྷἑ㈼୍ࡉࡺࡾ୰๓ᙪ㻘㻌 J. Soc. Cosmet. Chem. Jpn., 40(3), 195-200, (2006) 3) Tomohiro Imura et al. Chem. Eur. J. 12, 2434–2440, (2006,) ㅰ㎡ ࠶࠸ࡕࢩࣥࢡࣟࢺࣟࣥගࢭࣥࢱ࣮ࡢ 2014 ᖺᗘ๓ᮇᡂᯝබ㛤↓ൾ⏝ᴗࢆ ᐇࡍࡿ࠶ࡓࡾ㸪࠶࠸ࡕࢩࣥࢡࣟࢺࣟࣥගࢭࣥࢱ࣮ࢥ࣮ࢹࢿ࣮ࢱ࣮ࡢ㔝ᓮ Ặࡣไᗘࡢ⤂ࡽᛂເ㸪ᐇࡲ࡛ᵝࠎ࡞ࢻࣂࢫࢆ࠸ࡓࡔࡁࡲࡋࡓࠋࡲ ࡓබ┈㈈ᅋἲேᏛᢏ⾡ὶ㈈ᅋ 㔝ᮏ㇏༤ኈ㸪ຍ⸨୍ᚨ༤ኈࡣ ᐃࡢᐇ ࡞ࡽࡧゎᯒ㛵ࡋ࡚ࢻࣂࢫࢆ࠸ࡓࡔࡁࡲࡋࡓࠋࡲࡓྠ㈈ᅋඵ⏣୍㑻ඛ⏕ ࡣࢹ࣮ࢱࡢゎ㔘࡞ࡈᣦᑟ࠸ࡓࡔࡁ῝ࡃឤㅰ࠸ࡓࡋࡲࡍࠋ
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