pTK-Red Firefly Luc

PTK
A
Hp
BG
Te
r
TPS
01
c
La
Red F
irefl
yL
uc
0 O ri/
Pro m oter
pTK-Red Firefly
Luc
pUC Ori
ro
m
R
p
yc
Am
in R
SV4
(6718 bp)
Pu
SV40 pA
The following features are present in the vector
based on the nucleotide sequence.
Thymidine kinase (TK) promoter ................26-777
Red Firefly luciferase gene ....................790-2436
BGH poly(A) signal................................ 2448-2573
SV40 origin/promoter........................... 2574-3138
Puromycin resistant gene .................... 3139-3738
SV40 poly( A) signal ............................. 3900-3933
beta-lactamase (AmpR) gene ............... 4042-4902
pUC replication origin (pUC Ori)........... 5081-5885
Transcriptional terminator (Ter) ........... 5886-6493
Lac operator 1 (Lac O1) ....................... 6494-6514
Transcriptional pause site (TPS) .......... 6647-6718

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