pTK-Green Renilla Luc

PTK
A
Hp
BG
Te
r
PS
1T
c0
a
L
Green
Ren
illa
Lu
c
0 O ri/
Pro m oter
pTK-Green Renilla
Luc
pUC Ori
ro
m
R
p
yc
Am
in R
SV4
(6004 bp)
Pu
SV40 pA
The following features are present in the vector
based on the nucleotide sequence.
Thymidine kinase (TK) promoter ................26-777
Green Renilla luciferase gene ................791-1725
BGH poly(A) signal................................ 1734-1859
SV40 origin/promoter........................... 1860-2424
Puromycin resistant gene .................... 2425-3024
SV40 poly(A) signal .............................. 3186-3219
beta-lactamase (AmpR) gene ............... 3328-4188
pUC replication origin (pUC Ori)........... 4367-5171
Transcriptional terminator (Ter) ........... 5172-5779
Lac operator 1 (Lac O1) ....................... 5780-5800
Transcriptional pause site (TPS) .......... 5933-6004

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