pTK-Gaussia-Dura Luc

PTK
Lu
c
A
Hp
BG
Te
r
PS
1T
c0
a
L
Gauss
ia-D
ura
0 O ri/
Pro m oter
pTK-Gaussia-Dura
Luc
pUC Ori
ro
m
R
p
yc
Am
in R
SV4
(5626 bp)
Pu
SV40 pA
The following features are present in the vector
based on the nucleotide sequence.
Thymidine kinase (TK) promoter ................26-777
Gaussia-Dura luciferase gene ................790-1347
BGH poly(A) signal................................ 1356-1481
SV40 origin/promoter........................... 1482-2046
Puromycin resistant gene .................... 2047-2646
SV40 poly(A) signal .............................. 2808-2841
beta-lactamase (AmpR) gene ............... 2950-3810
pUC replication origin (pUC Ori)........... 3989-4793
Transcriptional terminator (Ter) ........... 4794-5401
Lac operator 1 (Lac O1) ....................... 5402-5422
Transcriptional pause site (TPS) .......... 5555-5626