PTK Lu c A Hp BG Te r PS 1T c0 a L Gauss ia-D ura 0 O ri/ Pro m oter pTK-Gaussia-Dura Luc pUC Ori ro m R p yc Am in R SV4 (5626 bp) Pu SV40 pA The following features are present in the vector based on the nucleotide sequence. Thymidine kinase (TK) promoter ................26-777 Gaussia-Dura luciferase gene ................790-1347 BGH poly(A) signal................................ 1356-1481 SV40 origin/promoter........................... 1482-2046 Puromycin resistant gene .................... 2047-2646 SV40 poly(A) signal .............................. 2808-2841 beta-lactamase (AmpR) gene ............... 2950-3810 pUC replication origin (pUC Ori)........... 3989-4793 Transcriptional terminator (Ter) ........... 4794-5401 Lac operator 1 (Lac O1) ....................... 5402-5422 Transcriptional pause site (TPS) .......... 5555-5626
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