Single Molecule Sequencing Course May 22-23, 2014

Single Molecule Sequencing Course
May 22-23, 2014 - KU Leuven
Organized by:
Matthew Hestand, Jeroen van Houdt and Joris Vermeesch in collaboration with IAP Medical Genomics, SymBioSys, and the KU Leuven Genomics Core
PROGRAM
Day 1: TECHNOLOGY
Thursday, May 22, 2014 - room BMW1 (University Hospital Leuven, Building Onderwijs en Navorsing II - ground floor)
09:00-09:30
Coffee & Registration (University Hospital Leuven, Onderwijs en Navorsing II - 6th floor)
09:30-10:30
Introduction to Single Molecule Sequencing
Liesbet Lagae (Imec, BE)
10:30-11:00
Genome mapping: applications in genome finishing and
analysis of structural variation at the single molecule level
Peter Verhasselt (VIB Nucleomics Core, BE)
11:00-11:30
Coffee break
11:30-12:00
PacBio Technology
12:00-13:00
Workshop Discussion: Challenges of Single Molecule Sequencing KU Leuven Panel
13:00-14:00
Lunch
14:00-14:30
Introduction to SMRT Analysis
Jeroen van Houdt (KU Leuven, BE)
Christoph Konig (PacBio)
Hands on Computer Workshop (computer room: PC-Lokaal Anatomie 2 in UZ Leuven, Building Onderwijs en Navorsing II)
14:30-15:30
15:30-16:00
16:00-17:00
PacBio data analysis -A
Coffee
PacBio data analysis -B
Matthew Hestand (KU Leuven, BE)
&
Christoph Konig (PacBio)
Day 2: APPLICATIONS
Friday, May 23, 2014 - room BMW1 (University Hospital Leuven, Building Onderwijs en Navorsing II - ground floor)
09:30-10:30
TBA
TBA
10:30-10:45
Long Read Phasing to Infer Disease Causing Mutations
Femke Hannes (KU Leuven, BE)
10:45-11:00
PacBio Sequencing of the FMR1 Repeat
Simon Ardui (KU Leuven, BE)
11:00-11:30
Coffee break
11:30-12:00
Single Molecule Sequencing of Free DNA from
Maternal Plasma for Non-invasiveTrisomy Detection
Elles Boon (LUMC, NL)
12:00-13:00
Deciphering the complete sequence and
global methylation state of genomes
Yahya Anvar (LGTC/LUMC, NL)
13:00-14:00
Lunch
14:00-15:00
De Novo Full-Length Transcriptome Analysis
by a Hybrid Sequencing Approach
Wei Chen (MDC Berlin, DE)
15:00-15:30
Long Amplicon Sequencing of Genomic Rearrangements
Matthew Hestand (KU Leuven, BE)
15:30-16:00
Coffee break
16:00-16:15
Closing Remarks
Joris Vermeesch (KU Leuven, BE)
16:15-17:00
Demonstration: BluePippin & PacBio RSII
Matthias Declercq (KU Leuven, BE)

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