WEDNESDAY 16 APRIL 2014 08:30 - 09:00 Registration 09:00 - 10:30 Parallel sessions Athene B/C Ecology and evolution of antibiotic resistance Chairs: Arjan de Visser & Willem van Schaik Non-lethal selection of antibiotic resistance Dan Andersson (Sweden) Parallel evolution of cefotaxime resistance in experimental populations of Escherichia coli Martijn Schenk The epidemic spread of Extended-Spectrum Beta-Lactamase-carrying plasmids among Escherichia coli from different hosts 09:00 - 09:30 09:30 - 09.45 09.45 - 10.00 10:00 - 10:15 10:15 - 10:30 Athene A 09:00 - 09:30 09:30 - 10:00 10:00 - 10:15 10:15 - 10:30 Room 4/5 09:00 - 09:30 09:30 - 10:00 10:00 - 10:15 10:15 - 10:30 Room 6/7 09:00 - 09:15 09:15 - 09:30 09:30 - 09:45 09:45 - 10:00 10:00 - 10:30 Room 8/9 09:00 - 09:15 09:15 - 09:30 09:30 - 09:45 09:45 - 10:00 10:00 - 10:15 10:15 - 10:30 10:30 - 11:00 Mark de Been Decrease of antimicrobial resistance in E. coli from animal husbandry reflects the reduction of antibiotic usage in animals in The Netherlands Dik Mevius Mining microbial metatranscriptomes for expression of antibiotic resistance genes under natural conditions Dennis Versluis Werkgroep MALDI-TOF en NVMy: Update on microbial diagnostics – application of MALDI-TOF Chair: Karola Waar The role of MALDI-TOF MS in the diagnosis of invasive fungal infections Maurizio Sanguinetti (Italy) Typing below the species level using MALDI-TOF mass spectrometry - applications and limits Oliver Bader (Germany) Performance of MALDI-TOF MS in identification of yeasts: a multi-center study Anneloes Vlek Potential misidentification of Staphylococcus pseudintermedius using MALDI-TOF MS and the development of a real time PCR Koen Verstappen Glycobiology in health and disease Chairs: Clara Belzer & Nina van Sorge The intestinal mucus and its crosstalk with bacteria, stimulation and protection Malin Johansson (Sweden) C-type lectins shape innate and adaptive immunity to bacteria and fungi Theo Geijtenbeek Sortase-based pull downs identify proteins involved in Dectin1 signaling Karin Strijbis Structural information of the complex of staphylococcal superantigen-like protein 3 and toll-like receptor 2 Kirsten Koymans Bachelor and Master (BAMA) Symposium Chair: Bas Zaat Phosphate stress and induction of the stringent response cause upregulation of mycobacterial capsular polysaccharides Maroeska Burggraaf Molecular mechanisms of LukM/F’ interaction with its G-protein coupled receptor CCR1 Dani Heesterbeek A Lipooligosaccharide Silencing RNA regulates Campylobacter jejuni pathogenicity Dior Beerens Using culturomics to isolate travellers-related antibiotic resistant bacteria from stool samples Joyce Aarts Detection and killing of resistant S. aureus using antimicrobial peptides Joep Kooijman Pathogenesis 1 Chair: Pieter-Jan Haas Matrix metallopeptidase 9 is induced by muramyl dipeptide in an NOD2 dependent manner and can be counteracted by lipopolysaccharide stimulation Marloes Vissers Proteolytic events involved in the regulation of CSS ECF sigma factor activity in Pseudomonas aeruginosa Karlijn Bastiaansen Characterization of YajC a transmembrane protein involved in cell wall stability and biofilm formation in Enterococcus faecium Fernanda Paganelli Lack of pAp phosphatase reduces Streptococcus pneumoniae virulence by negatively affecting de novo lipid synthesis and thus membrane homeostasis Kirsten Kuipers The C5 convertase is the docking site for Membrane Attack Complex assembly on the bacterial surface Evelien Berends Efficient phagocytosis of Aspergillus fumigatus through antibody-dependent complement activation Steven Braem Coffee/tea break 11:00 - 12:30 Parallel sessions Athene B/C Pathogenesis 2 Chair: Wilbert Bitter T-Cell tropism of simian Varicella virus during primary infection Werner Ouwendijk Human TLR10 is an anti-inflammatory pattern recognition receptor Marije Doppenberg-Oosting Identification of the staphylococcal Haemolysin-gamma receptors András Spaan The role of the mycosin protease in type VII secretion of pathogenic mycobacteria Vincent van Winden A Passenger or the Driver? A PPE substrate of the Mycobacterial secretion system ESX-5 is crucial for protein secretion via this system. Louis Ates Modelling tuberculous meningitis in zebrafish using Mycobacterium marinum Lisanne van Leeuwen 11:00 - 11:15 11:15 - 11:30 11:30 - 11:45 11:45 - 12:00 12:00 - 12:15 12:15 - 12:30 Athene A 11:00 - 11:30 11:30 - 12:00 12:00 - 12:30 Room 3 11:00 - 11:15 11:15 - 11:30 11:30 - 11:45 11:45 - 12:00 12:00 - 12:15 12:15 - 12:30 Room 4/5 11:00 - 11:15 11:15 - 11:30 11:30 - 11:45 11:45 - 12:00 12:00 - 12:15 12:15 - 12:30 Room 6/7 11:00 - 11:15 11:15 - 11:30 11:30 - 11:45 11:45 - 12:00 12:00 - 12:15 12:15 - 12:30 Room 8/9 11:00 - 11:30 11:30 - 11:45 11:45 - 12:00 12:00 - 12:15 12:15 - 12:30 NWKV & WAMM: Clinical cases in medical microbiology: an interactive session Chairs/moderators: Ed Kuijper, Saskia Kuipers, Ann Vossen & Rolf Vreede Alcohol and blisters? Madelon Engel Out of Africa with a headache Daphne Scoop Acid-fast? Jakko van Ingen Tuberculosis: New NVMM Diagnostic Guideline and nontuberculous mycobacteria Chairs: Jakko van Ingen & Bert Mulder Drug susceptibility testing of non-tuberculous mycobacteria: useful for treatment guidance? Ed Kuiper Quick molecular diagnosis of (resistant) M. tuberculosis according to the revised NVMM guidelines Sarah Vainio The secondary laboratory diagnosis of tuberculosis in the Netherlands Dick van Soolingen Identification of nontuberculous myco-bacteria by MALDI-TOF Arjan Jansz Utility of rpoB gene sequencing for identification of nontuberculous mycobacteria in the Netherlands Rina de Zwaan Development and evaluation of the detection of Mycobacterium tuberculosis complex on the BD-MAX system in a European multicenter study Ingrid op den Buijs Microbiome Chair: Birgitta Duim Effects of selective digestive decontamination on the gut microbiota in Intensive Care Unit patients Teresita de Jesus Bello Gonzalez The intestinal microbiome of phytopathogenic root fly larvae Cornelia Welte Congruency of phylogenetic composition and activity patterns in the human small intestine Tom van den Bogert Functional metagenomic analysis of genes conferring resistance to the disinfectant benzalkonium chloride in the human gut microbiota Elena Buelow Gut microbiota continues to evolve during first five years of life Anat Eck Dysbiosis of upper respiratory tract microbiota in elderly pneumonia patients Wouter de Steenhuijsen Piters Bachelor and Master (BAMA) Symposium Chair: Marie-Monique Immink The generation of a highly effective model to study the convertases of the complement system Hatice Orhan Chlamydial load in patients whom have naturallycleared Chlamydia trachomatis compared to non-cleared patients Marieke Franssen Butyrate-producing bacteria related to intestinal diseases Denise Vodegel Reducing turn-around time in positive blood cultures Mandy Kuijstermans Enrichment and isolation of sulfate-reducing bacteria associated with methanotrophic archaea Daan van Vliet The immunomodulatory effect of probiotic bacteria fractions Olaf Perdijk Viral infections in immunocompromised patients Chair: Anne Wensing Importance of immunereconstitution and viral infections: Towards more predictable immune-reconstitution after HCT Jaap-Jan Boelens Human herpesvirus-6 DNAemia is a sign of impending primary CMV infection in CMV sero-discordant renal transplantations Coretta van Leer-Buter Central nervous system infections with JC virus in immunocompromised patients Jean-Luc Murk Gene expression analysis reveals an important role for neutrophils in infants with severe viral respiratory tract infections Inge Ahout Human Polyomavirus 9 infection emerges in immunocompromized patients Herman Wunderink 12:30 - 14:00 Lunch Athene B/C 12:45 - 13:45 Satellite Symposium BD Athene A 13:00 - 14:00 BBC-MMO Business meeting 14:00 - 15:30 Parallel sessions Athene B/C General microbiology Chair: Stanley Brul Unraveling the environmental significance of nitrite-dependent anaerobic methane oxidation: a lipid biomarker approach 14:00 - 14:15 14:15 - 14:30 14:30 - 14:45 14:45 - 15:00 15:00 - 15:15 15:15 - 15:30 Athene A 14:00 - 14:30 14:30 - 15:00 15:00 - 15:15 15:15 - 15:30 Room 3 14:00 - 15:00 15:00 - 15:30 Room 4/5 14:00 - 14:30 14:30 - 15:00 15:00 - 15:15 15:15 - 15:30 Room 6/7 14:00 - 14:30 14:30 - 14.45 14.45 - 15.00 15:00 - 15:15 15:15 - 15:30 Room 8/9 14:00 - 14:30 14:30 - 15:00 15:00 - 15:15 15:15 - 15:30 Dorien Kool Streptococcus pneumoniae modulates human respiratory syncytial virus infection in vitro and in vivo Tien Nguyen Time resolved monitoring of Bacillus spore coat protein cross-links, spore germination and spore thermal resistance Stanley Brul Rare earth metals are essential for methanotrophic life in volcanic mudpots Huub op den Camp No gain, but also no pain? Selective disadvantage of carrying an IncI1 plasmid with blaCTX-M-1 genes in absence of antimicrobials Egil Fischer TnSeq in Streptococcus suis demonstrates that NADH oxidase is essential during infection of piglets Astrid de Greeff WAMM, WMDI & NWKV: Laboratory of the Future Chairs: Rolf Vreede & Els Wessels Automated microbiological diagnostics: the next step? Ed IJzerman Molecular microbiology 7 day testing, triple the workload and half the staff but managing to deliver faster service Kate Templeton (United Kingdom) Development of a rapid molecular test for diagnosis of bloodstream infections Martine Bos Automated monitoring of bacterial microcolony growth Alice den Hertog Risk Management: another step ahead! Chair: Eric van der Vorm Integral risk management John Prooi Infection prevention; same goal and together strong! Greet Vos A closer look at the Far East: medical microbiology in South-East Asian 'Tigers' Chairs: Menno de Jong & Henri Verbrugh Challenges and priorities in Southeast Asian medical microbiology Heiman Wertheim Novel Remedies for Deficiencies in Medical Microbiological Services in SE Asia Constance Schultsz Acquisition of ESBL- and carbapenemase-producing Enterobacteriaceae in a large prospective cohort of healthy travellers: The Carriage of Multiresistant Bacteria After Travel (COMBAT) study Maris Arcilla Targeted resistome detection reveals high acquisition rates of ESBL and quinolone resistance genes in the gut microbiota after international travel Christian von Wintersdorff Dientamoeba fragilis infections: clinical relevance, diagnosis and treatment Chair: Titia Kortbeek & Bert Mulder Dientamoeba fragilis in children in Denmark: aspects on transmission, epidemiology and management Dennis Röser (Denmark) Treatment of Dientamoeba fragilis: Indications and possibilities Tom van Gool Molecular detection of Dientamoeba fragilis shows a significantly higher rate in healthy controls than in gastroenteritis cases Bert Mulder Case-Control study Gastro Enteritis: The value of molecular detection in diagnosing gastro enteritis Lesla Bruijnesteijn van Coppenraet Tba tba Bacterial vaccines: from failures to promises Chairs: Wouter Jong & Ben van der Zeist Staphylococcus aureus vaccines: from failure to promise Fabio Bagnoli (Italy) Novel vaccines based on bacterial outer membrane vesicles Peter van der Ley An autotransporter display platform for the development of recombinant outer membrane vesicle vaccines Maria Daleke-Schermerhorn Antibody responses against non-covalent cell surface-bound staphylococcal proteins Francisco Romero Pastrana 15:30 - 16:00 Coffee/tea break Athene B/C 16:00 - 18.:00 NVMM Business meeting
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