Contact Person Paul Savelkoul P. Debyelaan 25 P.O. Box 5800 6202 AZ Maastricht, The Netherlands Phone +31 433 876 644 Fax +31 433 876 643 [email protected] Target Audience Up to 100 MDs, biologists, post-doctoral fellows and PhD students who are working in the field of microbiology and are interested in molecular diagnostics. Confirmed Faculty Members Martin Altwegg, Lucerne, Switzerland Eric Claas, Leiden, The Netherlands Tanja Geelen, Maastricht, The Netherlands Margareta Ieven, Antwerp, Belgium Hans Kusters, Utrecht, The Netherlands Colin Mackenzie, Düsseldorf, Germany Marlies Mooij, Maastricht, The Netherlands Jacob Moran-Gilad, Jerusalem, Israel Bert Niesters, Groningen, The Netherlands Roel Nijhuis, Velp, The Netherlands John Penders, Maastricht, The Netherlands Marijke Raymaekers, Hasselt, Belgium John Rossen, Groningen, The Netherlands Paul Savelkoul, Maastricht, The Netherlands Kate Templeton, Edinburgh, United Kingdom Anton van Loon, Utrecht, The Netherlands Rob Willems, Utrecht, The Netherlands Carl Wittwer, Salt Lake City, United States Petra Wolffs, Maastricht, The Netherlands Administrative Secretariat Secretary Office Dept. Medical Microbiology Maastricht UMC P. Debyelaan 25 P.O. Box 5800 6202 AZ Maastricht, The Netherlands Phone + 31 433 876 644 Fax +31 433 876 643 [email protected] Picture outside: Aerial view of the Maastricht University Medical Centre (MUMC) © ESCMID, September 2013 Contact ESCMID Postgraduate Education Course Principles of Molecular Microbiological Diagnostics Maastricht, The Netherlands 5 – 7 March 2014 ESCMID Postgraduate Education Course Principles of Molecular Microbiological Diagnostics Organisers • ESCMID Study Group for Molecular Diagnostics (ESGMD) • Institute of Medical Microbiology and Hospital Hygiene, Heinrich Heine University, Düsseldorf, Germany • University Hospital Antwerp, Antwerp, Belgium • Ministry of Health, Jerusalem, Israel • Bioanalytica AG, Lucerne, Switzerland • Edinburgh Specialist Virology Centre, Edinburgh, United Kingdom • Maastricht University Medical Centre, Maastricht, The Netherlands • University Medical Center Groningen, Groningen, The Netherlands • Leiden University Medical Center, Leiden, The Netherlands • Jessa Hospital, Site Virga Jesse, Hasselt, Belgium Course Coordinators • Tanja Geelen, Maastricht, The Netherlands • Marlies Mooij, Maastricht, The Netherlands • Paul Savelkoul, Maastricht, The Netherlands • Petra Wolffs, Maastricht, The Netherlands Course Objectives The course provides an overview of the general principles of state-of-the-art molecular techniques for diagnosis of infectious diseases: Quantitative PCR, (Next-Generation) Sequencing and Mass Spectrometry. In addition, the course educates participants on the set-up, validation and quality control of the different techniques and discusses the latest and upcoming developments in the field. Course Programme Wednesday, 5 March 2014 08:30 Welcome 09:00 Real-time PCR: the basics. Carl Wittwer Applications of real-time PCR 09:30 Quantitative PCR. Eric Claas 10:00 Multiplex PCR to maximum. Kate Templeton 10:30 Coffee Break 11:00 Gene expression profiling. To be announced 11:30 High Resolution Melting. Carl Wittwer 12:00 Digital PCR. To be announced 12:30 Lunch 13:30 PCR design & computer simulations. To be announced 14:00 PCR parameters for optimisation. To be announced 14:30 Troubleshooting for PCR. To be announced 15:00 Coffee Break 15:30 Molecular detection of antibiotic resistance and virulence factors . Roel Nijhuis 16:00 Point of care detection systems. Bert Niesters 19:30 Welcome dinner Thursday, 6 March 2014 09:00 Next-Generation Sequencing: the basics. To be announced Applications of Next-Generation Sequencing (NGS) 09:30 The use of pyrosequencing for typing and resistance. Rob Willems 10:00 Microbiome analysis. To be announced 10:30 Coffee Break Organisation 11:00 11:30 12:00 12:30 13:30 14:00 14:30 15:00 15:30 16:00 Setup of NGS workflow. To be announced Sample handling for NGS. John Penders Troubleshooting NGS workflow. John Rossen Lunch Advanced PCR detection: possibilities. Paul Savelkoul Application of micro-arrays. To be announced Broad Spectrum PCR for clinical diagnostics. Martin Altwegg Coffee Break Mass Spectrometry: the basics (types and principles). To be announced Applications of Mass Spectrometry in medical microbiology. Hans Kusters Friday, 7 March 2014 09:00 09:30 10:00 10:30 11:00 11:30 12:00 13:00 DNA / RNA isolation & sample processing. Petra Wolffs One-assay approach and principles of CE-IVD (in vitro diagnostics). Margareta Ieven Quality assurance (internal/external). Anton van Loon Coffee Break Guidelines molecular diagnostics. Marijke Raymaekers Molecular diagnostics: when to use and when not to use. Jacob Moran-Gilad The future developments in molecular diagnostics. Paul Savelkoul Closing lunch & presentation of new equipment by companies Course Venue Maastricht University Faculty of Health Medicine and Life Science Universiteitssingel 50 6229 ER Maastricht Maastricht, The Netherlands Registration Procedure Register now online on the ESCMID website at www.escmid.org/education. Registration deadline is 31 January 2014. Registration Fee EUR 500 for ESCMID members EUR 600 for all others The fee includes the scientific sessions, meals and all social events. Travel and accommodation are not included. Attendance Grants ESCMID provides a number of attendance grants for ESCMID “young scientist members”. The grant covers the registration fee, but not accommodation or travel costs. Please apply via the ESCMID website at www.escmid.org/education before 8 January 2014. Applicants will be informed about their acceptance by 22 January 2014. CME Accreditation The organisers of the course will apply for European CME accreditation through EACCME.
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