DiseasemodelgenerationbyDNArecombination R e l i a b l esourceoff u n c t i o n a lhumanneurons D i f f e r e n t i a t i o ni n t odopaminergicneurons DevelopedbyReproCELLusingKyotoUniversity' stechnology !fl• ReproCELL http: //www.reprocell.com/en ReproNeuro http: //www.reprocell.com/en ReproCELLhasdevelopedReproNeuro,humaniPScell-derivedneuralprogenitorsfromcollaborationwith KyotoU n i v e r s i t y .ReproNeuroi sar e l i a b l esourceoff u n c t i o n a lhumanneuronsthatcanbef l e x i b l yt a i l o r e dfor yourpurpose. 回 Developmentofdiseasemodelneuronstailoredforyourresearch 「 H aa 由m H︿ LFOO 石田区 1 n a t i o n 的出ち一 ↓… C o n t r o l 圃園田坦,~~皿fl:.1喧:~1・--- D i s e a s e DG : D i s e a s e r e l a t e dg e n eo fy o u ri n t e r e s t m o d e l WT M u t a n tl M u t a n t2 ReproCELLhasdevelopedthetechnologytoe a s i l yandr e l i a b l yintegrateexogenousgenesi n t o theiPSc e l lgenomebyusingarecombinationcassette.Thistechnologyallowedthegenerationof theAlzheimer ’ sdiseasemodelneurons( r i g h t ,dataprovidedbyKyotoUniversity).ReproNeuro canbet a i l o r e dtomakethediseasemodelneuronsofyouri n t e r e s t .Pleasecontactusfo「門1ore d e t a i l s . 四 Generationoffunctionalhumandopaminergicneurons ー 一 一一 ・~一一一一 ー一- Abouttwoweeksafterp l a t i n g ,theprogenitors d i f f e r e n t i a t ei n t odopaminergicneurons. B l u e :D A P I( n u c l e u s ) G r e e n :β 1 1 1 T u b u l i n( n e u r o nm a r k e r ) R e d :T y r o s i n eh y d r o x y l a s e( d o p a m i n e r g i cn e u r o nm a r k e r ) .曙E・ E・ ' " ・ ' H・ E担 制 c 0 me r . 宵F . l ' i 百四T 司F . T i F . 1 1 ・ E ・ 且 ! I ・ l i F . 1 : < =-m E 出 回S園 否[ 1 . 0・ ト お~ I c ; : . S0 . 8・ ト ~ §0.6十 三B0.4ト ・ ·~ ~ 0. 2・ ト γ0.0・ 」「 万 n os t i m u l a t i o n h i g hK C I Theinducedneuronscouldreleasedopaminewhenstimulated( l e f t ).Uptakeofdopamineanalogwasalso observedandinhibitedbyDAT-inhibitor( r i g h t ) .WithReproNeuro,youcanpreparefunctionalhuman neuronswheneveryouwant,insteadofusingc e l ll i n e sordissectinganimalstogetnon-humanneurons. CatalogNo. RCESDOlO Product No.ofIncludedc e l l s ReproNeuroDAk i t ( N e u r a lprogenitorsf o rdopaminergic neurong e n e r a t i o n ) Smallpack{ 3xlOeSc e l l s ,RCESDOll)i salsoavailable. ( 1 × 10 6c e l l s / v i a l )× 3 3 × 106 cells/vial R!lim 璽置盟国盟咽 Headqua吋 er s R e p r o C E L L ,I n c . KDXS h i n y o k o h a m a3 8 1 B l d g8F 3 8 1 1, S h i n y o k o h a m a Kohoku k u, Yokohama Kanagawa222-0033 , Japan T e l :+ 8 145 ・4 75 ・3 887 F a x :+ 8 145 ・4 74 ・1 006 !fl• ReproCELL: ~掴 i n f o _r epr o@r eproc e l. c om :Europe :R e p r o C E L L ,I n c . :H a m i l t o nHouse ;MabledonP l a c e B l o o円1 S b u r y ;LondonWClH988 :U n i t e dKingdom T e l :+44( 0 ) 2 0 ・3 137 ・1 5 3 1 ;F a x :+44( 0 )20 ・7 554 ・8 501 NorthAmerica ReproCELLUSA , I n c . 560S .W i n c h e s t e r8 1 v d . STE500 SanJ o s e , CA9 5 128 USA T e l:l ・4 08 ・8 76 ・8 544 F a x: l ・4 08 ・5 72 ・5 679
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