スライド 1 - Nature Publishing Group : science

Supplementary Figure 1 a
MTT activity (% of control)
120
NB4
HL60
100
80
60
40
20
0
0
0.1
0.2
0.3
AZD6244 (mM)
0.4
0.5
Supplementary Figure b
HL60
1.2
Antagonism
Combination index (C.I)
Combination index (C.I)
1.2
1.0
0.8
0.6
0.4
NB4
Synergism
0.2
0.0
Antagonism
1.0
0.8
0.6
Synergism
0.4
0.2
0.0
IC50
IC75
Effect Levels
IC90
IC25
IC50
Effect Levels
IC75
Supplementary Figure 1 c
NB4
HL-60
75
control
MS-275 0.5mM
AZD6244 0.25mM
MS-275+AZD6244
Cells (%)
*
50
Cells (%)
75
50
control
MS-275 0.5mM
AZD6244 0.25mM
MS-275+AZD6244
*
25
25
*
*
0
0
pre-G1
G0/G1
S
G2/M
pre-G1
G0/G1
S
G2/M
Supplementary Figure 1 d
NB4
75
*
*
50
25
0
MS-275 (mM)
AZD6244 (mM)
-
0.25
-
0.5
-
0.25
0.25
0.25
0.5
0.25
Annexin V positive cells (%)
Annexin V positive cells (%)
HL-60
30
*
20
*
10
0
MS-275 (mM)
AZD6244 (mM)
-
0.25
-
0.5
-
0.25
0.25
0.25
0.5
0.25
Supplementary Figure 1 e
NB4
HL60
#2
#6
Supplementary
Figure 2
control
96±4 %
HL60
AZD6244 0.25 mM
46±3 %
control
97±1 %
NB4
AZD6244 0.25 mM
36±6 %
MS-275 0.5 mM
56±8 %
MS-275 0.5 mM
+AZD6244 0.25 mM
26±5 %
MS-275 0.5 mM
46±2 %
MS-275 1 mM
55±3 %
MS-275 1 mM
+AZD6244 0.25 mM
24±8 %
MS-275 1 mM
33±6 %
MS-275 0.5 mM
+AZD6244 0.25 mM
MS-275 1 mM
+AZD6244 0.25 mM
23±5 %
27±9 %
p-HistoneH3 (Ser10)
p-HistoneH3
IgG

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