BOSCH INSTITUTE ADVANCED MICROSCOPY FACILITY … Zeiss transmitted light, fluorescence, and deconvolution microscopy workshop As a Bosch AMF user, you can sign up for Short workshop includes how to set-up for microscope training transmitted light, fluorescence and deconvolution microscopy. Includes Zen Blue software training. Zeiss transmitted light, fluorescence & Max. attendees*, 5. Tues Feb 18th or Weds Feb deconvolution microscope workshop th 19 10 am – 12 noon. Rm S443, Anderson Stuart Half-day workshop includes transmitted light, Building, F13 fluorescence and deconvolution microscopy. Max. attendees, 4. Tue Sep 20, 10.30 am 12.30 or confocal 2.30 - 4.30 pm Leica pm SPEII microscope workshop Rm S443, Anderson Stuart Building, F13 multiShort workshop on confocal imaging includes colour and z-stack image acquisition. Max. attendees*, Tues Febworkshop 25th or Weds Feb 26th 10 Leica SPEII5.confocal am – 12 noon. Rm S443,on Anderson Stuart Building, Half-day workshop confocal imaging F13. Upright microscope suitable for imaging fixed includes multi-colour and z-stack image and slide-mounted specimens. Zeiss Deconvolution and ApoTome.2 Microscope Leica SPEII Confocal Microscope acquisition. Max. attendees, 4. Tue Sep 27, 10.30 am - 12.30 pm or 2.30 - 4.30 pm Rm S443, Anderson Stuart Building, F13 Zeiss LSM 510 Meta confocal microscope workshop Summer/Autumn 2014 AMF Training Workshops Zeiss 510 Meta confocal microscope Short LSM workshop on confocal imaging includes workshop single-, multi-track and z-stack image acquisition. Half-day Max. attendees*, workshop 5. Tues onMar confocal 4th or Weds imaging Mar 5th 10.00 am –single-, 12 noon.multi-track Rm S443, Anderson Stuart includes and z-stack Building,acquisition. F13. Inverted image Max.microscope attendees,- 4.supports Tues imaging of slide-mounted specimens and single Oct 4, 10.30 am - 12.30 pm or 2.30 - 4.30 pm,or multi-well dishes. Advanced trainingF13 on timeRm S443,culture Anderson Stuart Building, series acquisition, spectral imaging, linear unmixing and online emission fingerprinting available by appointment only. *per session www.sydney.edu.au/medicine/bosch/facilities/advanced-microscopy *per session Zeiss LSM 510 Meta Confocal Microscope BOSCH INSTITUTE ADVANCED MICROSCOPY FACILITY All microscope training workshops are conducted by the Core Facilities Manager or the Microscopy Officer and are open to registered users of the Bosch Institute Advanced Microscopy Facility (AMF) only. Places are offered on a first-come first-served basis. Catapulted tissue in collection cap ready for downstream processing Tissue after laser microdissection and catapulting To join the Bosch AMF, please go to the Bosch AMF website (see bottom of page), download and complete a current registration form including payment details and send to the Core Facilities Manager. Costs for access to the Bosch AMF in 2014 are listed on this form. To register for a workshop, please contact: Dr Louise Cole (Core Facilities Manager) Tel. No. 93512520 Email: [email protected] Dr Ying Ying Su (Microscopy Officer) Tel. No. 93515802 Email: [email protected] www.sydney.edu.au/medicine/bosch/facilities/advanced-microscopy PALM DuoFlex Combi Laser Microdissection Microscope workshops Half-day workshop on how to laser micro-dissect selected areas of non-fluorescent and fluorescent cells or tissue samples. Dissected samples are catapulted against gravity into collection caps, are contamination-free and therefore suitable and ready for further downstream molecular analyses of RNA, DNA and/or protein. Max. attendees*, 6. Tues Mar 11th or Weds Mar 12th, Rm N135, Anderson Stuart Building, F13 MetaMorph® software training One-day workshop on the basics of MetaMorph image analysis software. Learn how to segment useful data, how to threshold images, the use of image processing and analysis tools, and how to write simple journals in order to automate image analyses. Max. attendees*, 6. Tues Mar 25th. Rm S448, Anderson Stuart Building, F13 All workshops will be repeated on demand. Other workshops on advanced imaging methods and microscope-related topics will be advertised separately. For further information please contact Dr Louise Cole directly ([email protected]).
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