RFP an ntibody [3 3F5] Description Mouse mono oclonal [3F5]] to Red Fluo orescent Proteins Productt Type Primary antibody Specific city tested on mR RFP, mCherrry, mPlum, mOrange, m tdT Tomato, DsR Red Isotype IgG2a Applicattion Western blott: recommen nded starting concentratio on 1/1000 IF: recomme ended startin ng concentrattion 1/1000 Optimal diluttions/concen ntrations should be determ mined by the e end user. Relevan nce Fluorescent proteins (F FP) are po owerful toolss to study protein localization and a dynamiccs in living ce ells. In additio on to the pro ominent green fluore escent prote ein (GFP) from Aequorrea victoria, a red fluorescent protein p (DsR Red) was disc covered in th he coral Disccosoma sp. Mutagen nesis of the e red progenitor have resulted r in several monomeric red FPs (m mRFP1, mC Cherry, mOra ange, mPlum m, etc) which becam me attractive tools for a number n of ap pplications. Form Liquid; hybridoma supernatant Size 100 μl Storage e Buffer e: 0.01% Sod dium Azide Preservative Material safe ety datashee et (MSDS) for this producct: Sodium Aziide MSDS Storage e instructtions Shipped at ambient a room m temperaturre. Upon receipt store at +4°C. Stable for on ne year. Do not n freeze. Tested applicattions Western blot: primary an ntibody: 3F5 diluted 1/1000 incubation n at 4°C o.n. secondary y antibody: 1/2000 anti-m mouseAlexa647 . Sensitivity of mouse RF FP antibody 3F5 goes dow wn to 10 ng of pu urified RFP p protein. primary an ntibody: 3F5 diluted 1/1000, secondary antiibody: 1/2000 antti-mouse-Ale exa647 pplications, no ot for diagno ostic or therap peutic use. Only for research ap RFP antib body 3F5 (Mousse monoclonal) 1 Version 2014-02-21
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