The Fundamental Research to the Application of Systemic

KURENAI : Kyoto University Research Information Repository
Title
Author(s)
Citation
Issue Date
URL
The Fundamental Research to the Application of Systemic
Insecticides (II). The Absorption and Translocation of Several
Insecticides in Rice Plant by Soil Application
石黒, 丈雄; 斎藤, 哲夫
防虫科学 (1971), 36(1): 17-24
1971-02-28
http://hdl.handle.net/2433/158666
Right
Type
Textversion
Departmental Bulletin Paper
publisher
Kyoto University
防虫科学節 36 怨 -Ⅰ
2) Da
ut
er
man.W.
C･
,J
.
E.
Cas
i
da.J
.
B.
Knaa
k,
T.
Kowal
c
z
yk:I
.Agr
.Fo
o
dChe
桝.
,7
.1
8
8
(
1
9
5
9)
.
3) Me
t
c
al
f
,
A.
L.
,T.
R.
Fukut
o,R.
a.
Mar
c
h:I
.
Ec
o
n.En
t
o
mo
t
.
,5
0
,3
3
8(
1
9
5
7
)
.
4) 0'
Br
i
e
n.R.
D.
: To
x
l
'
c pho
s
L
)
ho
r
l
L
S Es
L
c
r
s
,
Ac
a
d.Pr
e
s
s
,Ne
w Yor
k,345(
1
9
6
0
)
.
5
) Kumada,K.
,0.
Sa
t
o,Y.
Ohs
umi
,S.
Oht
a:
So
i
LSc
I
'
.& Pl
on
LNut
r
.
,1
3
,1
51(
1
9
6
7
)
.
l
l
,
D.
L.
:I
.
Ec
o
n.Eni
o
mo
t
.
,5
8,2
4
9(
1
9
6
5
)
.
6
) Bu
7
) Bul
l
,D.
L.
,D.
A.
Li
ndque
s
t
,∫.
Ha
c
ks
kayl
o:
J.Ec
o
n.Ent
o
mo
L
･
,56
,1
2
9(
1
9
6
3)
.
8
) Ha
c
ks
kayl
o,J
･
,D.
L Bul
l
:I
.Agr
.Fo
o
d
Che
m.
,l
l
,4
6
4(
1
9
6
3
)
.
9) Zaye
d,S.
M.
A.
D.
,A.
Ha
s
s
e
n,I
.
M.
I
.
Fakhr:
BL
'
o
c
he
m.Phar
mac
o
L
.
,1
7,1
3
3
9(
1
96
8
)
.
s
i
da,
J
.
E.
,R.
K.
Cha
pma
n,T.
C.
Al
l
e
n:I
.
1
0) Ca
Ec
o
n.Ent
o
mo
1
.
,4
5,5
6
8(
1
9
5
2
)
.
t
z
i
n,L.
W.
,良.
K.
Chapman: I
.Ec
o
n.
l
l
) Ge
t
.
,5
2,1
1
6
0(
1
9
5
9
).
Ent
o
mo
t
z
i
n,L.
W.
,R.
K.
Cha
pma
n:I
.Ec
o
n.
1
2
) Ge
7
.
,5
3
,4
7(
1
9
6
0
)
.
EnL
o
mo
ki
,
M.
,H.
T.
Rey
nol
ds:I
.Ec
o
n.Ent
o
mo
t
･
,
1
3
) Za
5
4,5
68(
1
9
61
)
.
t
o,Y.
,J
.
Mi
yamot
o:Pe
s
t
i
c
f
d
cand Te
c
h1
4
) Sa
u
e
,1
6
,2
3(
1
9
6
7
)
.
nl
q
TheFundar
r
L
e
nt
alRe
8
e
ar
C
ht
ot
heAppl
i
c
at
i
onofSy8
t
e
mi
cl
n8
e
C
t
i
c
i
de
S(
Ⅰ
Ⅰ
)
.TheAbs
or
pt
i
o
n
andTr
ans
l
o
cat
i
o
nofSe
ver
alI
ns
e
c
t
i
c
i
de
si
n Ri
c
epl
antbySoi
lappl
i
c
at
i
o
n. Ta
ke
ol
s
t
HG
URO
★
andTet
s
uoSAI
TO(
Labor
at
or
yofAppl
i
e
dEnt
o
mo
l
o
gyandNe
mat
o
l
o
gy,Fa
c
ul
t
yofAgr
i
c
ul
t
ur
e,
Na
goyaUni
ver
s
i
t
y,Na
goya)Re
c
e
i
ve
dNove
mbe
r2,1
9
7
0
.Bo
t
yu.
Ka
gak
u3
6
,1
7,1
971
.
4. 浸透殺虫剤の施用法に関する基礎的研究 (
Ⅰ
Ⅰ
) 土壌処F
J
i
による各抑F
1透殺虫剤の水稲におけ
:
5
.l
l
.2
.受理
る浸透および移行について.石怨文雄+,荒藤哲夫 (
名古屋大学凸学部q
J山学救出)4
Vam
i do
t
hi
o
n,Di
me
t
hoa
t
e
,Di
s
ul
f
o
t
o
n,Thi
o
r
ne
t
o
nおよび Me
c
ar
bam の水m にわける浸透移
2
p-班誠化合物を用いて検討した. すなわち,上記薬剤の乳#･
J
'
和郎紋を水稲の移Wl
LT
:土地
行性を 3
表面に施用し,薬剤の浸透移行およびそれにともなう殺虫力を調べた.
その結果.Di
s
ul
f
ot
o
n は施用田後に水和の地下部に薬包が多く. 地下部への兆刑出道が早く, ま
たI
)
i
me
t
hoat
eは地上部および地下部の英丑はほぼ同じであり, 机物体内の泣述および移行が極め
て容易であると推窮きれる.
i do
m
t
hi
o
n の投透移行性はゆるやかであり.Thi
ome
t
onおよび Me
c
ar
bam の
これに対して Va
それは弱い.同じ施用法で行なったヒメトビウンカに対すろ殺虫試験による死出串の糊加は地上部
の茶毘の増加とほほ一致した.
c
ar
ba
m は土地吸新物が多くI
)
i
me
t
hoat
e
実験終了時 (
施用後 6日日)に糾､て Va
i do
m
t
hi
o
nと Me
は少なかった.
I
nt
r
oduc
t
i
on
si
nc
eSc
hr
adera
ndKt
i
kent
ha
lf
i
r
s
tf
oundt
he
s
y
s
t
e
mi
ca
c
t
i
onofor
gano
phos
phor
usi
ns
e
c
t
i
c
i
de
s
i
n1
9
3
5,ma
nye
xpe
r
i
ment
sha
vebe
e
n madeo
n
a
bs
or
pt
i
on,t
r
ans
l
o
cat
i
o
nandme
t
a
bo
l
i
s
mi
np
l
a
nt
s
wi
t
h var
i
o
usappl
i
c
at
i
on met
ho
ds
. Atpr
e
s
e
nt
,
s
y
s
t
e
mi
ci
ns
e
c
t
i
c
i
de
so
c
c
upy a ver
yi
mpo
r
t
ant
po
s
i
t
i
o
ni
npe
s
tc
o
nt
r
o
lf
oragr
i
c
ul
t
ur
alcr
o
ps
.
I
twaspr
o
ve
dby Davi
d(
1
9
5
2
)
,
8
日yat
om
i and
Sai
t
o(
1
9
6
7
)
7
)a
ndHa
r
r
i
s(
1
9
6
7
)
6
)t
ha
ti
ns
e
c
t
i
c
i
da
l
ef
f
e
c
toft
heappl
i
e
dc
he
mi
c
al
swe
r
ei
nf
l
ue
nc
e
d
Pr
e
s
e
ntAddr
e
s
s:Abur
ahiLa
bo
r
at
o
r
i
e
s
,
Shi
ono
gi
皮 Co.
,Shi
gaPr
e
f
e
c
t
ur
e
.
現在 :瓜即苑拳法薬株式会社油日ラボラトt
)-ズ
byt
het
ypeofs
oi
l
.Fur
t
he
r
,
Chi
nge
tat
.(
1
9
61
)
,
2
)
Be
nne
t
t(
1
9
4
9
)
1
)a
ndWe
ddi
nge
tot
.(
1
9
5
2
)
1
8
)a
nd
Tei
t
z(
1
9
5
4
)
1
8
)s
t
udi
e
da
bs
or
pt
i
o
n,t
r
ans
l
o
cat
i
on
and pe
r
s
i
s
t
e
nc
e oft
he s
e
ve
r
ali
ns
e
c
t
i
ci
de
sr
e･
s
pe
c
t
i
ve
l
yonea
c
hcr
o
ps
.The
yr
e
por
t
e
dt
hatt
he
abs
or
pt
i
on s
i
t
ei
n cr
op var
i
e
sa
c
c
or
di
ngt
ot
he
appl
i
c
a
t
i
o
nme
t
hodsofchem
ic
al
s
.
Thi
sr
e
por
ti
sc
o
nc
er
ne
d wi
t
ht
heabs
or
pt
i
on
andt
r
a
ns
l
∝at
i
onoft
hef
o
l
l
owl
ngf
i
veS
空
pl
abe
l
l
e
d
s
ys
t
emi
c or
ganophos
phor
us i
ns
e
c
t
i
ci
de
si
nr
i
c
e
pl
ant
s: Vami
dot
hi
on,Di
me
t
hoa
t
e,Di
s
ul
f
ot
on,
Thi
ome
t
o
nandMe
c
ar
ba
m.
Me
t
e
r
i
al
Sandmet
hods
Te
s
tf
ns
e
c
t
i
c
l
'
de
s:Sul
urpo
f
wder
,me
t
ha
no
land
1
7
防虫
科
学
ammoni
awe
r
eadde
dt
o2
2
.
4
mCi
/
gofS
空
pdi
me
t
hyl
pho
s
phi
t
e,whi
c
hwa
spur
c
has
e
df
r
o
m Sumi
t
omo
At
o
mi
cEner
gyI
ndus
t
r
i
e
s
,andt
hemi
xt
ur
ewa
s
s
t
i
r
r
e
dt
o make ammoni
um s
a
l
tof3
2
p0,
0di
met
hylt
hi
o
pho
s
phat
e
･
VAddi
t
i
o
n ofe
qul
mOl
ar
5chl
or
o3l
t
hi
a2
Nme
t
hylva
le
r
am
i dei
na
c
e
t
on
t
ot
her
ea
c
t
i
o
nmi
xt
ur
ega
veS
空
pVa
mi
do
t
hi
o
n,13)
0,
0di
me
t
hy
lS[
2
1
(
1
me
t
hylc
ar
bamoyl
e
t
hyl
t
hi
o)
e
t
hy
l
⊃phos
phor
ot
bi
o
l
at
e
･
57
mCi
/
gofpo
t
as
s
i
um s
ltof8
a
2
pdi
me
t
hyldi
t
hi
o
･
phos
pha
t
e wa
s di
s
s
o
l
ve
di
na
c
e
t
o
n and adde
d
equl
mO
l
arNme
t
hyl1
c
hl
or
oa
c
e
t
oa
i deore
m
t
h･
yl
me
r
capt
oe
t
hylc
hl
or
i
det
ogi
ve8
2
pDi
me
t
ho
at
e
'
0,
0di
me
t
hyl S-(
Nme
t
hyl car
bamoy
l
met
hyl
)
pho
s
phor
odi
t
hi
oa
t
eand3
2
pThi
omet
on,
0,
0di
me
.
t
hylSE(
2
e
t
hyl
t
hi
o
)
et
hyl
jphos
phor
odi
t
hi
oat
e
･
54
.
5
mCi
/
g of pot
as
s
i
um s
lt of S
a
2
pdi
e
t
hyl
di
t
hi
o
phos
phat
ewasdi
s
s
o
l
ve
di
nac
e
t
o
na
ndadde
d
e
qul
mOl
aret
hyl
mer
ca
pt
o
e
t
hylc
hl
or
i
deorchl
or
o･
a
c
et
ylNmet
hylur
e
t
n t
a
og
ive8
2
pDi
s
ul
f
ot
o
n,
0,
0di
et
hylS-(
2
e
t
hyl
mer
c
apt
o)e
t
hylphos
phor
odi
t
hi
oa
t
e and a
2
pMe
c
ar
bam,0,
0di
e
t
hylS(
Net
hoxyc
ar
bo
nyl
Nme
t
hylcar
bamo
yl
met
hyl
)
phos
phor
o
di
t
hi
oat
e
.
Ea
c
hc
ompo
und wa
sdi
s
s
o
l
vedi
nc
hl
or
of
or
m,
andwas
he
ds
uf
f
i
ci
e
nt
l
ywi
t
hwat
e
randc
onc
e
n･
t
r
at
e
dt
odr
yne
s
s
.Pur
i
t
yofe
ac
hc
ompo
undwas
de
t
er
mi
ne
d by me
ansofdi
s
t
r
i
but
i
on ofr
adi
o･
a
c
t
i
vi
t
yi
ns
i
l
i
c
a ge
lt
hi
n･
l
ayerc
hr
omat
o
gr
aphy
and c
o
l
or
i
me
t
r
i
c de
t
e
r
mi
nat
i
o
n wi
t
h pa
l
l
adi
um
c
hl
or
i
de
. Someoft
hec
ompo
undswe
r
epur
i
f
i
e
d
f
ur
t
he
rwi
t
ht
hi
n･
1
ayerc
hr
omat
o
gr
apby.
Eac
hcompo
undwasaddedt
oac
e
t
on:be
nz
e
ne:
Ne
wc
oa18
6
3(
manuf
ac
t
ur
e
dbyJ
apanEmul
s
i
f
i
e
r
co
.
,Lt
d.
) at
:3
0:
3
0:
3
0 W/
W and ma
de l
O
%
emul
s
i
f
i
abl
ec
o
nc
e
nt
r
at
i
o
n.
Tc
s
りL
ant
:Twohundr
e
dgr
am ofai
r
dr
i
e
dpaddy
f
i
e
l
ds
oi
lwhi
c
h waspas
s
e
dt
hr
o
ugh as
i
e
veof
5mm wer
eputi
n agl
a
s
spe
t
r
idi
s
hof1
8c
mi
n
di
amet
e
randadde
dwat
erofpr
es
cr
i
be
dquant
i
t
y
t
oi
t
･Fi
f
t
e
e
ns
t
ands(
3pl
a
nt
spe
rs
t
and)ofr
ic
e
s
e
e
dl
i
ng (
Ai
c
bi
･
As
a
hi
)att
hr
e
el
e
afs
t
a
gewe
r
e
t
r
ans
pl
a
nt
e
di
ngl
a
s
spe
t
r
idi
s
h.
､
A♪Pl
i
c
at
t
o
n o
fr
adi
o
ac
t
i
v
ec
o
mL
'
o
unds t
oy
i
c
e
n pe
r
c
e
nte
mul
s
i
f
i
abl
ec
onc
e
nt
r
at
i
o
n
bt
ant
s:Te
wasdi
l
ut
e
dat0
.
0
5pe
r
c
e
ntwi
t
hdi
s
t
i
l
l
e
dwat
e
r
.
,
Af
t
e
r2daysf
r
o
mt
r
ans
pl
a
nt
i
ngoft
her
i
c
epl
ant
s
t
hedi
l
ut
eds
o
l
ut
i
o
nofea
c
hc
ompo
undwa
sdr
o
ppe
d
1
8
鍔 36 巻-I
o
nt
ot
hes
ur
f
a
c
eofs
oi
lase
ve
nl
yaspo
s
s
i
bl
ew
it
h
,
api
pe
t
t
eatt
her
at
eof2
0
mJ(
as･
a
c
t
i
vei
ngr
e
di
e
nt
1mg/
pe
t
r
idi
s
h=3
.
6kg/
1
0 ar
e
si
nt
er
m o
f5%
gr
anul
e
)
.Thepl
ant
swe
r
enotdi
r
e
c
t
l
yc
ont
ami
･
,
ma
t
e
dwi
t
ht
hes
o
l
ut
i
o
n.Dur
i
ngt
hee
xper
i
me
nt
s
t
her
i
c
epl
ant
swer
eke
pti
n agr
o
wt
hc
ha
mber
t
ha
twe
r
es
e
twi
t
ha1
0
W f
l
uor
or
e
c
e
ntl
ampat
2
50
C,a
nds
uppl
i
e
dv
(
at
e
rt
oma
i
nt
ai
nt
hewat
er
l
.
s
ur
f
a
c
eatac
o
ns
t
antl
e
ve
Sampl
i
n
g:Ats
e
ver
l t
a
i
me i
nt
e
r
va
l
s af
t
e
rt
he
a
ppl
i
c
at
i
o
n,t
hr
e
es
t
andsoft
her
i
c
epl
ant
swer
e
s
ampl
e
d at r
a
ndam i
n or
de
rt
oe
xa
mi
ne t
he
di
s
t
r
i
but
i
onofr
a
di
o
a
c
t
i
veme
t
abo
l
i
t
e
si
nt
her
i
c
e
pl
a
nt
. Ther
oo
t
swe
r
ewas
he
ds
uf
f
i
c
i
e
nt
l
ywi
t
h
ar
unni
ng wat
e
rt
o r
e
mo
ve t
he unabs
o
r
be
d
i
ns
e
c
t
i
c
i
de
s
.Thet
r
e
a
t
e
dr
i
c
epl
ant
swer
edi
vi
de
d
i
nt
ot
he ae
r
i
aland r
o
ot par
t
s
,and wei
ghe
d
r
e
s
pe
c
t
i
ve
l
yaf
t
e
rr
e
mo
vi
ngt
hemo
i
s
t
ur
ewi
t
ha
f
i
l
t
e
rpa
pe
r
.
Af
t
ermi
nc
i
ngt
hem,
t
hemat
e
r
i
a
l
swer
egr
i
nde
d
wi
t
h2
mJo
fdi
s
t
i
l
l
e
dwat
e
ri
nagl
a
s
sho
mo
ge
ni
z
e
r
ands
ha
ke
di
tvi
gor
o
us
l
ywi
t
hs
a
meamo
unt
sof
chl
o
r
of
or
m.Ho
mo
ge
nat
e
soft
hea
e
r
i
a
landr
o
o
t
par
t
swer
ec
e
nt
r
i
f
uge
df
o
r5mi
nut
e
sat2
0
0
0r
･
p･
m･
t
os
e
par
at
ec
hl
or
of
or
ml
ayerf
r
om wat
e
rl
aye
r
.
Ont
heot
he
rhand,
al
lt
r
e
at
e
ds
oi
l
swe
r
ec
ol
l
e
c
t
e
d
f
r
om t
hepet
r
idi
s
hr
e
s
pe
c
t
i
v
e
l
ya
t6daysa
f
t
er
t
hea
ppl
i
c
at
i
o
n and e
xt
r
a
c
t
e
dt
hr
e
et
i
me
swi
t
h
80
% ac
e
t
o
nei
nwat
er
.Thee
xt
r
a
c
t
swer
ec
o
nc
e
n･
t
r
a
t
e
dwi
t
h ar
ot
ar
ye
vapor
at
orunderr
e
duc
e
d
pr
e
s
s
ur
e,andt
he
npar
t
i
t
i
o
ne
dwi
t
hequa
lvol
ume
ofc
hl
or
of
or
m anddi
s
t
i
l
l
e
d wa
t
e
r
. Chl
or
of
or
m,
wa
t
e
re
xt
r
a
c
t
sands
oi
l
･
abs
or
be
ds
ubs
t
a
nc
e
swe
r
e
s
e
pa
r
at
e
dbyt
heabo
vede
s
c
r
i
be
do
pe
r
at
i
o
n.Ea
c
h
f
r
a
c
t
i
o
nwasc
o
nc
e
nt
r
at
e
dwi
t
har
ot
a
r
ye
vapor
at
o
r
unde
rr
educ
e
dpr
e
s
s
ur
eagai
n.
Radi
o
as
s
a
y:Tot
a
lamo
unt
sofc
hl
or
of
or
me
xt
r
a
c
t
s
(
unde
c
o
mpo
s
e
dpr
oduc
t
),wat
e
re
xt
r
a
c
t
s(
de
c
om･
po
s
e
d pr
o
duc
t
) and pr
e
c
i
pi
t
a
t
e
s we
r
e put i
n
s
ampl
i
ngdi
s
he
sf
orr
adi
oa
s
s
a
yr
e
s
pe
c
t
i
ve
l
y,a
nd
pour
e
d2
% KOI
i
a
l
c
o
ho
ls
o
l
ut
i
o
ni
nt
ot
he
m.And
t
he
n,e
a
c
hf
r
a
c
t
i
o
nwasdr
i
e
dunde
ra
ni
nf
r
ar
e
d
l
amp. Thepr
e
c
i
pi
t
at
e
swe
r
ei
nc
i
ne
r
at
e
di
nan
e
l
e
c
t
iCf
r
ur
na
c
eat5
0
0o
C.Ther
adi
o
a
c
t
i
vi
t
i
e
sof
ea
c
hf
r
a
c
t
i
o
nwe
r
eme
as
ur
e
dbyt
heAl
o
kagas
･
f
l
ow c
ount
e
r
.
Bi
o
as
s
a
yfo
rb
y
ownz
I
L
ant
ho
b♪e
y:Ri
c
epl
ant
swhi
c
h
we
r
et
r
ea
t
e
dno
n･
l
a
be
l
l
e
dc
o
mpo
undsi
nt
hes
a
me
防虫科学邦 3
6 巻 -I
me
t
hodsast
hel
a
be
l
l
e
doÅe
swe
r
er
e
ho
ve
d at
6,2
4,4
8,7
2.9
6,1
2
0 and 1
4
4 ho
ur
s af
t
e
rt
he
ap
pl
i
c
a
t
i
on.Twopl
ant
soft
het
r
ea
t
edr
i
c
epl
a
nt
s
we
r
et
r
ans
f
er
r
e
di
nt
o at
r
ans
par
e
ntc
yl
i
nderof
3
5
mm i
ndi
a
met
erand2
0c
mi
nhe
l
'
h tma
g
deby
po
l
yet
hyl
e
ne f
i
l
m,and t
he
nf
i
xe
dw
it
hs
ponge.
Tenf
e
ma
l
e
sofadul
ts
ma
l
l
e
rbr
ownpl
ant
ho
pper
(
De
t
bhac
o
dc
ss
t
n'
at
e
L
t
aFa
ll
e
n)
.
whi
c
hwe
r
er
ear
e
d
f
o
rs
uc
c
es
s
i
ve ge
ne
r
a
t
i
o
nso
nr
i
c
epl
a
nt
si
nour
l
a
bo
r
at
or
y,wer
er
e
l
e
a
s
e
di
nt
ot
hecyl
i
nde
r
.The
c
y
l
i
nde
r
swe
r
eke
pti
nagr
o
wt
hc
l
l
a
mbe
ra
t2
5o
C.
Thenumbe
roff
al
l
e
ni
ns
e
c
t
swascount
e
daf
t
e
r
2
4and 4
8hour
s
,a
nd t
hec
or
r
e
c
t
e
d pe
r
c
e
nt
age
wa
sc
a
l
uc
ul
a
t
edbyt
heAbbo
t
'
sc
or
r
e
ct
i
o
nme
t
hod.
Thei
ns
e
c
t
i
c
i
dalt
e
s
t
swer
er
e
pl
i
c
at
e
dt
hr
e
et
i
me
s
.
Re
8
ul
t
8
I
nor
de
rt
os
t
udyabs
or
pt
i
o
nandt
r
a
ns
l
oc
at
i
o
n
,
o
fs
ys
t
e
mi
cpho
s
phor
usi
ns
e
c
t
i
c
i
de
si
nr
i
c
epl
a
nt
s
S
空
pl
a
be
l
l
e
d co
mpo
undswer
e appl
i
e
d ont
os
oi
l
s
ur
f
a
c
e
.Af
t
e
rt
heappl
i
c
at
i
o
n,t
her
i
c
epl
ant
swe
r
e
s
a
mpl
e
dats
eve
r
ali
nt
e
r
val
sf
o
rt
her
adi
o
as
s
ay.
The decr
e
me
nto
fr
adi
oa
c
t
i
vi
t
y wasc
or
r
e
c
t
ed
a
ndt
heamountofr
adi
o
a
c
t
i
vemat
e
r
i
a
l
si
nt
he
a
e
r
i
alandr
oo
tpar
t
sofr
i
c
epl
a
ntwascal
c
ul
at
ed
i
nt
e
r
m ofi
ni
t
i
alc
ompo
unds
. The amount
sof
t
her
adi
oa
c
t
i
vemat
e
r
i
a
l
si
nt
her
i
c
epl
ant
sbe
t
we
e
n
chl
or
of
or
m and wat
e
re
xt
r
a
c
t
s ar
es
ho
wn i
n
Tabl
e
s1and2
.
Asr
e
gar
dst
ot
hepe
ne
t
r
at
i
o
noft
hec
o
mpo
unds
i
nt
ot
he r
o
ot of r
i
ce p
l
a
nt
s
,t
he amount
so
f
c
hl
or
of
or
me
xt
r
a
c
t
a
bl
eme
t
abo
l
i
t
e
sofDi
s
ul
f
ot
o
n,
Vami
do
t
hi
o
n,Thi
o
me
t
o
nandMe
car
bam at
t
ai
ne
d
ma
xi
mumwi
t
hi
nonl
y6ho
ur
saf
t
ert
heappl
i
c
at
i
o
n,
anddi
dno
ti
ncr
eas
eaf
t
er
war
ds
.Ont
hec
ont
r
ar
y,
c
hl
o
r
o
f
or
me
xt
r
a
c
t
a
bl
eme
t
abo
l
i
t
e
sofDi
me
t
hoa
t
e
i
nc
r
ea
s
e
d gr
adua
l
l
y wi
t
ht
he l
a
ps
e of t
i
me
.
Di
me
t
hoat
ewaspr
ove
dt
oha
ve anat
ur
eoft
he
s
t
r
o
nge
s
tpe
ne
t
r
at
i
o
namongt
het
e
s
tc
o
mpounds
.
Vami
d
o
t
hi
o
n s
ho
wed r
api
d i
nc
r
eas
e i
n t
he
a
c
umul
at
e
d amount of c
hl
or
of
or
m e
xt
r
a
ct
abl
e
me
t
a
bo
l
i
t
e
si
nt
hea
er
i
alpar
t
sofr
i
c
epl
ant
sf
r
o
m
4
8hour
saf
t
ert
heappl
i
c
at
i
on. Ther
adi
oa
c
t
i
ve
me
t
abo
l
i
t
e
s pa
r
t
i
t
i
one
di
n aque
ousphas
e we
r
e
i
ncr
e
as
e
dwi
t
ht
hel
a
ps
eoft
i
me,andt
hel
ar
ge
amount
sofi
t
sme
t
abo
l
i
t
e
swe
r
ef
oundi
nal
lt
e
s
t
c
ompoundsat4
8hour
saf
t
e
rt
heappl
i
c
at
i
on.
Tabl
e3S
ho
wst
hec
onc
e
nt
r
at
i
onofc
hl
or
of
or
m
par
t
i
t
i
one
dme
t
abo
l
i
t
e
si
nt
her
i
c
epl
ant
satc
er
t
ai
n
t
i
mei
nt
er
val
saf
t
e
rt
heappl
i
cat
i
o
n,andt
hedat
a
Tabl
e1
. Theamount
sofr
adi
o
a
c
t
i
vema
t
e
r
i
a
l
sf
o
undi
nt
her
o
otpar
t
soft
hr
e
eri
c
epl
ant
s
(
1s
t
and)a
f
t
ert
hea
ppl
i
c
at
i
o
nofS
空
pl
abe
l
l
e
di
ns
e
c
t
i
ci
de
s
.
Te
s
tc
ompounds
I
Fr
a
c
t
i
o
n
Va
mi
dot
bi
o
n
c
hl
or
of
or
me
xt
r
ac
t
s
wa
t
e
rext
r
a
c
t
s
une
xt
r
ac
t
a
bl
e
Di
me
t
hoa
t
e
c
hl
or
o
f
or
me
xt
r
a
c
t
s
wa
t
e
rext
r
a
c
t
s
une
xt
r
ac
t
a
bl
e
Di
s
ul
f
ot
on
c
hl
or
o
f
or
me
xt
r
a
c
t
s
wa
t
e
re
xt
r
a
c
t
s
une
xt
r
a
c
t
a
bl
e
Thi
ome
t
o
n
c
hl
or
of
or
me
xt
r
a
c
t
s
wa
t
e
re
xt
r
a
c
t
s
une
xt
r
a
c
t
abl
e
Me
c
ar
bam
c
hl
or
of
o
r
me
xt
r
a
c
t
s
wat
e
re
xt
r
a
c
t
s
une
xt
r
a
c
t
abl
e
6hr
s
.
Ti
me a
f
t
e
ra
p
p
l
i
cati
o
n
2
4hr
s
. 14
8hr
s
. 172 hr
s
.
1
● Unde
t
e
c
t
a
bl
ebyt
hega
s
･
f
l
o
wc
o
unt
e
r
.
1
9
防虫科学弟 3
6 巻-1
Tabl
e2. Theamo
unt
sofr
adi
oa
c
t
i
vemat
e
r
i
al
sf
o
undi
nt
heae
r
i
a
lpar
t
so
ft
hr
eer
i
c
e
pl
ant
s(
1s
t
a
nd)af
t
e
rappl
i
c
at
i
onofS
空
pl
a
be
l
l
e
di
ns
e
c
t
i
c
i
de
s
.
Tes
tc
o
mpo
unds
I
Fr
a
c
t
i
o
n
Vami
do
t
hi
o
n
c
hl
o
r
o
f
or
me
xt
r
a
c
t
s
wat
ere
xt
r
a
c
t
s
une
xt
r
ac
t
abl
e
Di
met
bo
at
e
c
hl
or
o
f
or
me
xt
r
a
c
t
s
wat
e
re
xt
r
a
c
t
s
une
xt
r
a
c
t
abl
e
Di
s
ul
f
o
t
o
n
c
hl
or
of
or
me
xt
r
a
c
t
s
wa
t
e
re
xt
r
a
c
t
s
une
xt
r
a
c
t
abl
e
Thi
ome
t
on
c
hl
o
r
of
or
me
xt
r
a
c
t
s
wat
e
re
xt
r
a
c
t
s
une
xt
r
a
c
t
abl
e
Me
c
ar
bam
c
hl
or
of
or
me
xt
r
a
c
t
s
wa
t
e
re
xt
r
a
c
t
s
une
xt
r
a
c
t
a
bュ
e
Ti
meaf
t
e
ra
ppl
i
c
at
i
o
n
s
. 124hrs.1
6hr
.1
48hr
s
72hr
s
.
★ Unde
t
e
c
t
a
bl
ebyt
hega
s
･
f
l
o
wc
o
unt
er
.
Tabl
e3. The c
o
nc
e
nt
r
at
i
on ofc
hl
or
o
f
or
m par
t
i
t
i
o
ne
d me
t
abo
l
i
t
e
si
nr
i
c
e pl
a
nt
s af
t
er
appl
i
c
at
i
o
nofa
2
pl
a
be
l
l
e
di
ns
e
c
t
i
c
i
des
.
Te
s
tc
ompo
unds
I Par
tofe
xt
r
a
c
t
i
o
n
Ti
meaf
t
erappl
i
c
at
i
o
n
6hr
s
. 12
4hr
s
. 14
8br
s
. 17
2hr
s
.
Dat
ae
xpr
e
s
s
e
dasi
ni
t
i
a
lc
o
mpoundspe
rf
r
e
s
hpl
a
ntt
i
s
s
ue
.
we
r
ee
xpr
e
s
s
e
da
si
ni
t
i
alc
ompoundspe
rf
r
e
s
h
pl
antt
i
s
s
ue
s
. Di
me
t
hoat
ee
xhi
bi
t
e
dt
hehi
ghe
s
t
co
nc
ent
r
at
i
o
ni
n bot
h par
t
s
,and f
o
l
l
o
we
d by
Di
s
ul
f
ot
on. Co
nc
e
nt
r
at
i
o
n ofc
hl
or
of
or
m pa
r
t
i
･
t
i
one
dmet
abo
l
i
t
e
si
nt
he ae
r
i
a
lpa
r
t
st
e
nde
dt
o
bet
hehi
ghe
s
tat4
8ho
ur
saf
t
ert
heappl
i
c
at
i
o
n
andgr
adua
l
l
yl
o
wer
eda
f
t
er
war
ds
.Co
nc
e
nt
r
at
i
on
2
0
ofi
t
smet
abo
l
i
t
e
si
nt
her
oo
tpar
t
sl
o
we
r
edwi
t
ht
he
l
aps
eoft
i
meaf
t
e
rt
hea
ppl
i
cat
i
o
no
fDi
s
ul
f
o
t
on,
Vami
do
t
hi
on,Thi
o
me
t
o
na
nd Me
c
a
r
bam exc
e
pt
Di
me
t
hoat
e
.
Re
s
i
dualamo
unt
soft
hei
ns
e
c
t
i
c
i
d
e
sa
ndt
hei
r
me
t
a
bo
l
i
t
e
si
ns
o
i
lat6daysaf
t
e
rt
heappl
i
c
a
t
i
on
ar
e
I
de
s
cr
i
be
di
nTa
bl
e4.Ther
e
s
i
duala
mo
unt
s
防虫科学節 3
6 巻 -I
Tabl
e4
. Theamount
sofr
adi
o
a
ct
i
ve mat
e
r
i
al
sf
o
undi
nt
r
eat
e
ds
oi
l
s(
2
0
0
g.
)at6days
af
t
era
ppl
i
c
at
i
o
n.
Te
s
tc
ompo
unds
I Chl
or
of
or
me
xt
r
a
c
t
s I
Wat
ere
xt
r
ac
t
s
Soi
l
･
abs
or
be
d
s
ubs
t
anc
e
Tabl
e5
.I
ns
e
c
t
i
c
i
da
lt
e
s
t
sofs
ys
t
e
mi
cor
ga
no
pbos
phor
usi
ns
e
c
t
i
c
i
de
st
ot
i
l
eS
mal
l
e
rbr
o
wn
De
l
♪hac
o
d
e
ss
L
y
i
at
e
l
l
a F.
)f
e
d on r
i
c
e pl
ant
st
r
eat
e
d wi
t
ht
he
pl
a
nt
ho
ppe
r(
i
ns
e
c
t
i
c
i
de
s
.
o
fc
hl
or
o
f
o
r
mpar
t
i
t
i
o
ne
dme
t
a
bo
l
i
t
e
sofDi
s
ul
f
o
t
o
n
and Di
me
t
hoat
e we
r
e ver
yl
ar
ge
,andf
o
l
l
o
we
d
f
ol
l
o
we
dbyMe
c
ar
ba
m.
Adul
t
soft
hes
ma
uerbr
o
wnpl
a
nt
ho
ppe
rwe
r
e
by Thi
o
me
t
on,Me
c
ar
ba
m and Vami
do
t
bi
o
ni
n
de
cr
e
as
l
ngOr
de
r
.Ont
heco
nt
r
ar
y,s
o
i
l
a
bs
or
be
d
s
ubs
t
anc
e
sofVami
dot
bi
on wa
st
hel
ar
ge
s
t
,and
f
e
d on t
l
l
er
i
c
epl
a
ntt
r
eat
e
dwi
t
hno
n･
l
a
be
l
l
e
d
s
a
mei
ns
e
c
t
i
c
i
de
s
.Ta
bl
e5S
ho
wst
hepe
r
c
e
nt
age
off
al
l
e
ni
ns
e
c
t
s at2
4and4
8hour
s af
t
e
rt
l
l
e
21
防虫科学第 3
6 巻 -Ⅰ
appl
i
c
at
i
on. Ther
i
c
epl
a
nt
st
r
e
at
e
dDi
met
ho
at
e
e
xhi
bi
t
edt
hebe
s
ti
ns
e
ct
i
c
i
da
le
f
f
e
c
t
,andf
ol
l
o
we
d
Vami
dot
hi
o
na
ndDi
s
ul
f
o
t
on.Ho
weve
r
,
Thi
ome
t
o
n
andMe
c
ar
bam ha
da
l
mos
tnoi
ns
e
c
t
i
c
i
da
le
f
f
e
c
t
As s
e
e
n･i
n Tabl
e
s 1 and 2,ho
we
ve
r
,t
he
pe
ne
t
r
at
i
onandt
r
ans
l
o
c
at
i
on ofVami
do
t
bi
oni
s
r
at
he
rs
l
o
werasc
ompa
r
e
dwi
t
hDi
me
t
boa
t
ea
nd
Di
s
ul
f
ot
o
n.
f
ort
hes
mal
l
e
rbr
o
wnpl
a
nt
ho
ppe
r
.
l
l
)
Ac
c
or
di
ngt
ot
heo
bs
e
r
vat
i
onofMi
yat
a(
1
9
6
5
)
o
nt
he pe
ne
t
r
at
i
on ofvar
i
ouski
ndso
for
ga
no･
pho
s
phor
us i
ns
e
c
t
i
c
i
de
si
nt
ot
he pl
antt
i
s
s
ue.
whe
nt
hei
ns
e
c
t
i
c
i
de
swe
r
ea
ppl
i
e
do
mr
i
c
epl
a
nt
s
wi
t
ht
her
oo
tdi
ppi
ngme
t
ho
d,S
o
me i
ns
e
c
t
i
ci
de
s
pe
ne
t
r
at
e
donl
y al
i
t
t
l
ei
nt
ot
hep
l
antt
i
s
s
uei
n
s
pi
t
eofi
t
shi
ghwat
e
r
･
S
ol
ubi
l
i
t
y,
a
nds
t
abi
l
i
t
yof
c
he
mi
c
al
s and pe
ne
t
r
a
t
i
on me
c
ha
ni
s
m gr
ea
t
l
y
i
nf
l
ue
nc
e
d on t
hepe
ne
t
r
at
i
onoft
hec
he
mi
c
a
l
s
i
nt
ot
hepl
a
ntt
i
s
s
uebe
s
i
de
st
hewa
t
er
･
S
o
l
ubi
l
i
t
y.
Ont
heot
he
rha
nd,Di
s
ul
f
o
t
on pe
ne
t
r
at
e
da
nd
t
r
ans
l
o
cat
e
dr
e
a
di
l
yi
nt
ot
hepl
antt
i
s
s
ueal
t
l
1
0ugh
i
twa
s al
i
t
t
l
es
ol
ubl
ei
n wat
e
r
.Ase
vi
de
nc
e
d
Di
S
C
u舶i
on
l
thasbe
e
ns
ai
dt
hata
l
lout
e
rs
ur
f
a
c
eo
fpl
a
nt
bo
dy c
an a
bs
o
r
b var
i
o
us ki
nds o
fc
o
mpounds
unde
rc
er
t
ai
nc
o
ndi
t
i
ons
. The
r
e
f
or
e,a
sf
ort
i
l
e
pene
t
r
at
i
o
nofs
ys
t
e
mi
ci
ns
e
c
t
i
c
i
de
si
nt
ot
hepl
ant
bo
dy,i
ts
ho
ul
d be t
a
ke
n i
nt
o c
ons
i
de
r
at
i
o
n
whe
t
he
rt
he s
ur
f
a
c
eo
ft
he pl
ant bo
dy ha
s
ada
pt
abi
l
i
t
yf
orabs
o
r
pt
i
o
no
ft
hec
ompo
unds
･
I
ti
sc
ons
i
de
r
e
dt
hati
ns
e
c
t
i
c
i
de
spe
ne
t
r
at
epl
ant
i
at
hes
amer
o
ut
ea
s
bodyf
r
om t
her
o
ots
ys
t
e
mv
wat
eroror
ga
ni
cmat
er
i
a
l
s
.
Whe
nt
he i
ns
e
c
t
i
c
i
de
sar
ea
ppl
i
e
do
n wat
e
r
s
ur
f
a
c
eofapaddyf
i
e
l
d,
t
hei
ns
e
c
t
i
c
i
de
sar
enot
onl
yabs
or
be
dv
i
ar
oot
sbuta
ls
o mo
ve
supwar
d
a
l
o
ngs
ur
f
a
c
eandi
nt
er
s
t
i
c
esofs
t
emorl
e
a
fs
l
l
e
at
h
bycapi
l
l
ar
i
t
y,andar
r
i
ve
si
nt
hes
t
e
mo
rl
ea
ve
s
unde
rs
uc
h as
pe
c
i
a
le
nvi
r
onme
ntoff
l
oodi
ng
(
I
s
i
i and Hi
r
ano,6) 1
9
6
2
,Tomi
z
a
wa,
m l
9
6
3
)
･
Ther
ef
or
e,t
he s
ys
t
e
mi
ci
ns
e
c
t
i
c
i
de
ss
ho
ul
d be
cont
a
ct
e
ddi
r
e
c
t
l
y wi
t
ht
hepl
a
ntbo
dyi
nor
de
r
t
oe
xpe
ctt
hehi
ghe
s
te
f
f
e
c
tf
ornoxi
ousi
ns
e
c
t
s
･
Me
t
c
al
f(
1
9
5
6
)
Vr
e
por
t
e
dt
hato
neo
ft
hef
a
c
t
or
s
t
oc
ont
r
olt
hepe
ne
t
r
at
i
on ofc
he
mi
c
al
si
nt
ot
he
pl
antt
i
s
s
uewa
si
t
swat
e
r
･
S
o
l
ubi
l
i
t
y,andt
hatany
c
he
mi
c
als
houl
d have t
hes
o
l
ubi
l
i
t
y eno
ughf
or
e
na
bl
i
ng i
tt
omovef
r
e
e
l
yl
n pl
antt
i
s
s
ue
s
･I
t
wasr
e
por
t
e
da
l
s
obyMe
t
c
al
fe
ial
l(
1
9
5
7),
1
0)Da
vi
d
(
1
95
2
)
3
)andRi
dgwa
ye
tal
･
14
)(
1
9
5
6
)t
ha
tt
r
ans
l
l
o
c
at
i
onofc
he
mi
c
al
si
nt
ol
e
a
ve
sa
nds
t
em de
pe
nd
upont
hel
i
pos
o
l
ubi
l
i
t
yandt
hats
ubs
t
anc
ehavi
ng
wat
er
･
s
o
l
ubi
l
i
t
yr
e
adi
l
ype
ne
t
r
at
et
hepl
a
ntt
i
s
s
ue
v
i
at
her
oo
t
.
f
r
om t
heabo
ver
e
s
ul
t
,t
het
r
ans
l
o
c
at
i
ngr
at
eof
t
hec
he
mi
c
a
l
sc
oul
dnotbej
udge
df
r
om onl
yi
t
s
wat
e
r
･
S
ol
ubi
l
i
t
y.
The amount of r
adi
oa
c
t
i
ve me
t
abo
l
i
t
e
si
n
Di
me
t
hoat
ewast
hel
ar
ge
s
tatt
hea
e
r
i
a
landr
o
ot
par
t
sofr
i
c
epl
a
nt
s
,and t
he amo
untgr
a
dua
l
l
y
i
ncr
e
a
s
e
d wi
t
h t
he l
a
ps
e of t
i
me a
f
t
e
rt
he
appl
i
c
a
t
i
on.Fr
om t
her
e
s
ul
t
,i
ti
spr
es
ume
dt
ha
t
Di
me
t
hoat
ei
saf
as
t
･
a
c
t
i
ng I
ns
e
c
t
i
c
i
deowi
ngt
o
r
e
adi
l
ype
ne
t
r
a
t
i
o
nandt
r
ans
l
o
c
at
i
o
ni
nt
hepl
a
nt
t
i
s
s
ue
s
.Ho
we
ve
r
,i
ti
ss
ugge
s
t
edt
ha
tDi
me
t
hoa
t
e
c
aus
e
sr
e
adi
l
yphyt
ot
oxi
c
i
t
yt
os
o
mepl
a
nt
ss
i
nc
e
i
tpe
ne
t
r
at
e
sa
ndt
r
ans
l
o
c
at
e
sveryr
a
pi
dl
yi
nt
he
pl
antt
i
s
s
ue
.
Sat
oandMi
ya
mo
t
o(
1
9
6
7
)
1
0
)
r
e
po
r
t
e
dt
hef
o
l
l
o
w･
i
ng r
e
s
ul
t
soft
he f
at
e ofDi
me
t
hoat
ei
ns
oi
l
:
Di
me
t
boa
t
et
r
a
ns
l
o
c
at
e
si
npl
antt
i
s
s
uewi
t
hs
o
i
l
moi
s
t
ur
e,
butt
het
r
ans
l
∝at
i
ngr
at
eo
fDi
me
t
hoat
e
i
sr
at
he
rs
ma
l
li
nt
hec
as
eofi
mmova
bi
l
i
t
yo
f
wat
e
re
ve
nwhe
nt
hes
o
i
l
c
o
nt
ai
nss
uf
f
i
ci
e
ntwat
e
r
.
Af
t
er
war
dsDi
me
t
bo
at
ei
sa
c
c
umul
a
t
e
dwi
t
ho
ut
wat
er
･
s
o
l
ubi
l
i
t
y of t
he c
ompo
unds us
e
df
or
t
hi
se
xpe
r
i
me
nti
sa
sf
ol
l
o
ws:Vami
do
t
hi
o
n2
0
g,
Di
s
ul
f
ot
on2
.
5mg,Di
met
hoat
e2
5
0mgpe
rl
O
OmJ
ofwat
e
rr
e
s
pe
c
t
i
ve
l
y･ Thi
o
me
t
o
ni
spr
a
c
t
i
c
al
l
y
l
i
ght
l
ys
o
l
ubl
e
i
ns
o
l
ubl
eand Me
c
ar
bam i
svery s
i
nwat
e
r
.Thus
,i
ti
spr
e
s
ume
dt
hatVami
do
t
hi
o
n
be
i
ng de
c
ompo
s
e
d and s
o Di
me
t
ho
at
ec
a
us
e
d
phyt
o
t
o
xi
c
i
t
y.
havi
ng t
hehi
ghe
s
twat
er
･
s
o
l
ubi
l
i
t
y amo
ng t
e
s
t
c
ompoundsi
sgr
ea
t
l
y abs
or
be
da
ndt
r
ans
l
o
c
at
e
d
v
J
'
arootsyst
e
mlbas
e
dont
her
e
por
t
sbyMet
c
al
f
e
Lat.
1
0
)andDa
vi
d.
3)
appl
i
e
d on wa
t
e
rs
ur
f
a
c
eofpoti
n whi
c
hr
i
c
e
pl
a
nthadbe
e
nt
r
ans
pl
ant
e
d.I
nge
ne
r
l,or
a
gano
.
phos
phor
us i
ns
e
c
t
i
c
i
de
s appi
t
e
di
n paddy f
i
e
l
d
a
l
mos
tr
e
mai
nont
hes
oi
l
s
ur
f
a
c
ewi
t
ho
utdi
f
f
us
i
ng
22
Tor
ni
z
a
wa (
1
9
6
3
)
1
7
)r
e
por
t
e
dt
hatdi
me
t
hylSi
s
o
pr
o
pyト2
s
ul
f
i
nylpbo
s
phor
ot
hi
o
l
a
t
ei
sa
bs
o
r
be
d
v
i
al
e
afs
he
at
ha
nd t
r
ans
l
o
c
at
ei
nt
ol
e
a
fbl
ade
,
buti
ta
l
s
omo
ve
st
or
oo
ti
naf
ai
r
l
ya
mount
,whe
n
防虫科学節 3
6 番-I
i
nt
ot
hel
o
werl
aye
rofs
oi
l
:
I
nt
hi
se
xpe
r
i
me
nt
,undert
hec
ondi
t
i
on t
hat
wa
t
e
r wa
s exc
e
s
s
i
ve
l
ys
at
ur
at
e
d, r
a
di
oa
c
t
i
ve
me
t
ab
ol
i
t
e
sofDi
me
t
hoat
emove
dt
ot
heaer
i
al
pa
r
t
s of r
i
c
e pl
ant ver
y r
a
pi
dl
y.and we
r
e
ac
c
umul
at
e
di
nt
hel
e
afs
he
at
handl
eafb一
a
dei
n
. Fr
o
m t
he
s
er
e
s
ul
t
.i
ts
e
e
ms
al
ar
ge amount
agai
ns
tt
hes
mal
l
e
rbr
ownpl
ant
ho
ppernott
obe
duet
ot
hea
c
c
umul
at
e
damo
untofea
c
hc
he
mi
c
al
s
,
att
heae
r
i
a
lpar
t
soft
her
i
c
epl
ant
s
.h ge
ner
al
t
hei
ns
e
c
t
i
ci
dehavi
ngnat
ur
e
sofs
t
r
ongpe
ne
t
r
at
i
on
andt
r
ans
l
o
cat
i
o
nhasal
s
ot
hehi
ghe
s
ti
ns
e
c
t
i
c
i
dal
pot
e
nc
y. I
ns
e
c
t
i
c
i
da
lef
f
i
c
a
cy o
ft
hec
t
l
e
mi
c
al
s
wasi
nac
c
o
r
d wi
t
ht
her
es
i
dua
lamo
untoft
l
l
e
pr
o
babl
et
hatDi
me
t
hoat
ewasnotonl
yabs
or
be
d
t
hr
ough r
o
ot
sbuta
l
s
ope
ne
t
r
at
e
dt
hr
oughl
e
af
s
he
at
handt
r
ans
l
o
c
at
e
di
nt
hepl
a
ntt
i
s
s
ue
s
.
c
he
mi
c
al
si
nt
l
l
eae
r
i
a
lpar
t
soft
hepl
ant
.
A l
ar
ge amountof c
hl
or
o
f
or
m par
t
i
t
i
o
ne
d
me
t
abo
l
i
t
e
sofDi
me
t
boat
ewa
sde
t
e
c
t
e
di
nt
he
Theabs
or
pt
i
o
nandt
r
a
ns
l
o
cat
i
o
noff
i
ves
ys
t
e
mi
c
i
ns
e
c
t
i
ci
de
s
,Vami
do
t
hi
o
n,
Di
s
ul
f
o
t
on,
Di
me
t
hoat
e
,
Thi
o
me
t
o
n and Me
c
ar
ba
m i
nr
i
c
e pl
ant
swe
r
e
qua
nt
i
t
at
i
ve
l
yandqual
i
t
a
t
i
ve
l
ys
t
udi
e
dbyus
i
ng
8
2
Pl
abe
l
l
e
di
ns
e
c
t
i
c
i
de
s
. Ri
c
es
e
e
dl
i
ngs we
r
e
c
ul
t
ur
e
di
nt
hepa
ddyf
i
e
l
ds
oi
lc
ont
ai
ne
di
nt
he
gl
as
spe
t
r
idi
s
h,andt
hel
abe
l
l
e
di
ns
e
c
t
i
c
i
de
swer
e
appl
i
e
dont
ot
hes
oi
ls
ur
f
a
c
e
.
Re
s
ul
t
soft
hee
xpe
r
i
me
nt
ss
ho
we
dt
hatDi
s
ul
･
f
ot
o
nwasabs
or
be
dr
api
dl
y by t
her
oot
soft
he
r
i
c
es
e
e
dl
i
ng,andl
ar
geamount
sofDi
me
t
hoat
e
wasabs
or
be
d by t
heae
r
i
alpar
t
saswe
l
lasby
t
her
oot
s
.
ae
r
i
a
landr
oo
tpar
t
sasc
ompar
e
dwi
t
hot
he
rt
e
s
t
c
o
mpo
unds
. The
r
e
f
or
e
,i
ti
s pr
e
s
ume
d t
hat
Di
me
t
hoat
ewasde
c
ompo
s
e
di
nt
hepl
a
ntt
i
s
s
ue.
1
I
o
we
ver
,Mor
i
ka
waandSai
t
o(
1
9
6
6)1
2
)andSat
o
a
nd Mi
ya
mot
o(
1
9
6
7
)
1
5) r
e
por
t
e
d,asar
e
s
ul
tof
s
t
udy on me
t
abo
l
i
s
m ofDi
me
t
hoat
e,t
hat t
he
c
o
mpoundcanno
tber
ea
di
l
yde
c
ompos
e
di
nt
he
l
e
a
ve
sofr
i
c
epl
a
ntands
oi
l
.
Theamo
untofr
adi
oa
c
t
i
vemat
er
i
a
l
sofDi
s
u1
f
o
t
o
n,Va
mi
dot
hi
on,Thi
ome
t
o
n and Me
c
ar
bam
i
nt
her
oo
tpar
t
st
e
nde
dt
obeke
ptal
mo
s
tc
ons
t
ant
o
rs
l
i
ght
l
yl
o
werwi
t
ht
hel
aps
eoft
i
meaf
t
e
rt
he
a
ppl
i
c
at
i
o
n.Fr
om t
l
1
0
S
er
e
s
ul
t
s
,
i
ts
e
e
mspr
oba
bl
e
t
hatt
her
o
ota
bs
or
bf
ur
t
hert
hec
hemi
cal
st
o
s
uppl
e
me
nt t
he de
c
r
ea
s
e
d amo
unt af
t
er t
he
mo
ve
me
nto
fc
he
mi
c
al
si
nt
ot
heae
r
i
alpar
t
s
,
s
i
nc
e
amo
untoft
hec
he
mi
c
al
supt
a
ke
nf
r
om r
oo
ti
s
a
l
mo
s
ti
nvar
i
abl
e
.
Ac
c
or
di
ngt
ot
hei
nve
s
t
i
gat
i
o
nofr
e
s
i
duei
ns
oi
l
at6da
ysaf
t
ert
heappl
i
c
at
i
o
n,c
hl
o
r
of
or
m and
wa
t
e
rpa
r
t
i
t
i
o
ne
dme
t
a
bo
l
i
t
e
sofDi
met
ho
at
eand
Di
s
ul
f
ot
o
ns
t
i
l
lr
e
mai
ne
di
ns
oi
li
nal
a
r
geamo
unt
.
On t
he c
o
nt
r
ar
y,t
he amo
unt ofc
hl
or
o
f
or
m
par
t
i
t
i
o
ne
dme
t
a
bo
l
i
t
e
si
nVami
do
t
hi
o
nwass
mal
l
butt
heamo
untofs
oi
l
･
a
bs
or
be
ds
ubs
t
a
nc
ewa
s
l
ar
ge
.
Ge
t
2
l
i
ne
tal
.(
1
97
0
)
l
)r
e
por
t
e
dPho
r
a
t
eappl
i
e
d
i
ns
oi
ls
ho
we
dgo
o
dpe
r
s
i
s
t
e
nc
e
,
a
ndt
ha
ts
ul
f
oxi
de
a
nds
ul
f
o
neofPhor
at
ewe
r
es
t
r
ongl
yabs
or
be
d
bys
oi
lc
o
mpone
nt
s
. Di
me
t
hoat
ehavi
ngna
t
ur
e
s
o
fgo
odpe
ne
t
r
at
i
o
n and t
r
ans
l
o
c
at
i
o
nwasbe
s
t
i
nt
hei
ns
e
c
t
i
c
i
da
le
f
f
e
c
tagai
ns
tt
hes
mal
l
e
rbr
own
pl
a
nt
I
1
0
ppe
randi
twa
sf
o
l
l
o
we
dbyVami
do
t
hi
o
n
andDi
s
ul
f
o
t
on.Thi
o
me
t
o
nandMe
c
a
r
ba
mpr
ove
d
t
obepooref
f
e
c
t
. Thi
si
sc
o
ns
i
de
r
e
dt
obedue
t
ot
hei
ns
e
c
t
i
c
i
da
lef
f
e
c
ti
t
s
e
l
fo
fea
c
hc
ompound
･Summar
y
I
twasas
s
ume
dt
hatabs
o
r
pt
i
o
nandt
r
ans
l
o
cat
i
o
n
ofDi
me
t
ho
at
ei
nr
i
c
e pl
ant
swer
e ext
r
eme
l
y
e
as
y.Whi
l
et
r
ans
l
o
c
at
i
o
nofVami
dot
hi
onwhi
c
h
hasanat
ur
eofhi
ghwat
er
･
s
ol
ubi
l
i
t
ywasf
ound
t
obes
l
o
w.
Thet
r
ans
l
o
c
at
i
onoft
heche
mi
c
al
st
ot
heaer
i
a
l
par
t
swer
eas
s
∝i
at
e
dwi
t
ht
hei
ns
e
ct
i
ci
dalef
f
e
c
t
s
t
ot
he br
o
wn pl
ant
ho
ppe
r whi
ch at
t
a
c
ks r
i
c
e
pl
a
nt
s
.
Ac
knowl
e
dge
me
nt
8:Wewi
s
ht
ot
hankPr
of
.
Ki
s
a
bul
yat
o
mi
,Fa
c
ul
t
yofAgr
i
c
ul
t
ur
e,Nago
ya
Uni
ver
s
i
t
yf
o
rma
ny he
l
pf
uls
ugge
s
t
i
on dur
i
ng
t
hec
our
s
eo
ft
hi
se
xpe
r
i
me
ntandal
s
ot
oSumi
t
o
mo
Che
mi
c
a
l
sCo.
,Lt
d.
,Shi
o
nog
i & Co
.
,Lt
d.
,Ni
ho
n
To
kus
buNoya
kuCo.
,Lt
d.andTake
daChe
mi
c
al
I
ndus
t
r
i
e
sCo.
,Lt
d.f
o
rpr
ovi
di
ng us s
ynt
he
t
i
c
i
nt
e
r
me
di
at
e
soft
he t
e
s
tc
he
mi
ca
l
s
. Fur
t
he
r
,
t
ha
nks ar
et
e
nde
r
e
dt
o Mr
.Mi
c
hi
o Endo of
Sumi
t
o
moAt
omi
cEn色
r
gyl
ndus
t
ie
r
sf
orhi
sus
e
f
ul
advi
s
e
sons
ynt
he
s
i
sofr
adi
oa
c
t
i
vec
ompo
unds
.
Re
E
e
r
e
nc
e
S
1
) Be
nne
t
t
,S.
l
L:Ann. A♪♪L. Bl
'
o
l
.
,3
6,1
60
(
1
94
9)
.
2) Chi
ng,
H.
T.
,and E.
W.
Cl
ar
k:)
.Ec
o
n
.
2
3
防虫
科
学
EnL
o
mo
t
.
,5
4,1
2
2
8(
1
9
61
)
.
3) Davi
d,W.
A.
L.
:An
n
.AL
1
3
･
t
.Bz
'
o
l
.
,39
,2
0
3
く
1
9
5
2
)
.
4) Get
z
i
n,L W.a
ndC.
班.
Shank,Jr
.
:I
.
Ec
o
n
.
Ent
o
mo
l
.
,6
3
,5
2(
1
9
7
0
)
.
5) Har
r
i
s
,C.
R･
:I
.Ec
o
n
. Ent
o
mo
l
･
,6
0
,41
(
1
9
6
7
)
.
6) I
s
hi
i
,S.andC.
Hi
r
ano:'
J
a
♪.I
.o
fA♪Pl
.
EnL
o
mo
t
.Zo
o
l
.
,6
,2
7(
1
9
67
)
.
7) I
yat
omi
,K.andT.
Sai
t
o: Expe
r
i
me
nt
a
l
r
e
s
e
ar
c
hf
or t
he s
ys
t
e
mi
ci
ns
e
c
t
i
c
i
de
,4
(
1
9
6
7
)
.
l
･
.Pat
.
,8
3
5,1
4
5(
1
9
5
4
)
.
8) J
onas
,H.
:Ge
l
f
,R.L.
: Se
c
ond i
nt
e
r
nat
i
o
na
lPl
ant
9) Me
t
c
a
Pr
ot
e
c
t
i
o
nConf
e
r
enc
e,Fer
nbus
t2
3(
1
9
5
6)
.
1
0) Me
t
c
al
f
,氏.L,E.M.
St
af
f
or
d,T.
R.
Fukut
o
第 3
6 巻 -I
B.
Mar
c
h:I
.Ec
o
n.En
t
o
mo
l
･
,5
0
.2
0
5
a
ndR.
(
1
9
5
7
)
.
l
l
) Mi
yat
a,T･
:Pr
o
c
.Kans
aiPl
.Pr
o
t
･So
c
･
,7
,
4
0(
1
9
6
5
)
.
1
2
) Mor
i
kawa,0.andT.
Sai
t
o:Bo
t
y
uKa
gak
u,
3
1
,1
3
0(
1
9
6
6
)
.
1
3
) Pi
anka,
M.
:Che
w.
Z
nd･
,
Mar
c
hll,
3
2
4(
1
9
61
)
･
1
4
) Ri
dg
way,
R.
L,
D.
A.
Li
ndqui
s
tandD･
LBul
l
:
J
.Ec
o
n.En
t
o
mo
l
･
,5
8
,3
4
9(
1
9
6
5
)
･
1
5
) Sat
o,Y.and∫
.
Mi
ya
mot
o:Pe
s
t
i
c
i
de and
Te
c
bni
que
,1
6
,2
3(
1
9
6
7)
.
1
6
) Te
i
t
z
,H.
:Ho
f
c
he
n･
Br
ief
e,7
.1(
1
9
5
4)
･
1
7
) Tomi
z
a
wa,C･
:J
a
♪.I o
fA♪
PL
･Ent
o
mo
L
･
Zo
o
l
.
,7
,1
4
0(
1
9
6
3
)
.
1
8
) We
ddi
ng,R.
T.and 氏.
L.
Me
t
c
al
f
: Bo
L
a〟
･
Gaz
.
,I
l
l,1
8
0(
1
9
5
2
)
.
TheCompar
at
i
vePher
omoneAc
t
i
vi
t
yofAc
e
t
at
e
80ft
T
nB
at
l
l
r
at
e
dA
lc
o
l
1
0
1t
oMa)
e
BOEt
he
Al
mondMot
h.Shoz
oTAKAHAS
Hl
,Chi
ka
yos
hiKI
TAMURA and Yas
umas
a KuwAHARA(
Pes
t
i
ci
de
Res
e
ar
c
hI
ns
t
i
t
ut
e,Col
l
e
geofAgr
i
c
ul
t
ur
e,Kyot
oUni
ve
r
s
i
t
y,Kyo
t
oJ
apan)Re
c
e
i
ve
d Januar
y
2
0,1
9
71
.Bo
t
yu
･
Ka
gak
u36,24,1
9
7
1
.(
it
w
hEng
li
s
hs
ummar
y2
6
)
5
.
スジマダラメイガに対する性フェロモン類縁化合物の活性について ★ 高橋正三
火彬,桑原探正 (
茄都大学
凸学瓢
北村
投薬研究施設)4
6
･1
･2
0
･受理
スジマダラメイガの蛾は性フェロモン郊緑化合物に対して, フェロモンの場合とは比較にならな
いほどの市娘庇ではあるがフェロモンに対すろ反応と同様の挙動を示す. それらの化合物の中では
柑こ,c
t
s
-7
t
r
i
de
c
en1
1
0la
c
e
t
at
eと c
i
s
l
9t
e
t
r
ade
c
e
n1
0
1a
c
e
t
at
eに対する反応が銃著であった.
既に鞭岱したように, スジマグラメイガ (
t
he
l
･
ac
auE
e
t
L
aWal
ke
r
)の性誘引物
al
mondmot
h,Cad
ェロモン活性の低い化合物も含まれているため節一次
校定として,Bu
t
e
na
ndtらの方法に準じて定性的なL
L
i
質は c
i
s
9
,L
r
ans
l
1
2
t
e
t
r
a
de
c
a
di
e
n1
01a
c
e
t
at
eで
あることが明らかとなった1
)
.
物検定を以下のように行なった2). 羽化後 3日日のス
今【
乱われわれは性フェロモン輝線化合物である高
径1
1
c
m,高さ 7
c
m)に入れ室温 2
80土 lo
C 条件下で
級不飽和アルコールおよびェポキシアルコールの酢髄
検定を行なった.供試化合物を細いガラス榊こつけ
エステル3
6
超を用いて生物検定を行ない,それぞれの
フェロモン活性を調べ,あわせて化学構造と活性との
(
約3
0
0
-5
0
0
F
L
g)ふたにあいた穴からシャーレの小に
5
-3
0
秒以内に超を特徴的に拡動させて動
挿入した.1
関係を解明することを目的として以下の実験を行なっ
きまわる反応を示す虫数を数えた.
ジマグラメイガ末交尾堆1
0-2
5匹を腰高シャーレ
(
山
2
) 第二次検定 :節一次検定で活性を示した化合物
た.
について,以前報告したフェロモン検定法E
i
P
l
l
i
を班用し
実験材料および方法
供試化合物 :検定に供した不飽和アルコールおよび
て一定血 (
1
0
0
pg あるいは 1
0i
L
g)の化合物に対すろ
pe
rc
e
ntr
e
s
po
ns
e
"を求めたS
)
.
雄の "
ェポキシアルコールの酢酸エステルは当研究室で合成
Ja
c
obs
o
n 博士く
U･
S･
D･
A･
)
されたもの, および M.
より捉供されたもの (
袈 1において化合物の肩に *印
をつけた) を用いた.
生物検定 : 1
)第一次検定 :供筑化合物が多く, フ
+本研兜の一部は文部省科学研究資によった.
2
4
実験結果および考察
髭 1に示す3
6
種の化合物を第一次検定にI
Jtした. 〟
0
0-5
0
0
pgの試料に対してス
ラス棒の先に付着した 3
ジマダラメイガ雄が反応した化合物には ○ 印をつけ
た.それぞれについて活性の強度を表 2に示した.分

Download Report