VAlid until 31st December 2014 life science special Every 3 months: attractive offers from GE Healthcare, MP Biomedicals, Biowest, LABSOLUTE, Heathrow Scientific, Vacuubrand, Binder, Greiner Life Science special Research and analyses and production processes in the life sciences require products of the highest quality. Th. Geyer‘s Life Science portfolio contains everything you need for reliable and reproducible results. You benefit from our many years of expertise in the domain of laboratory consumables and equipment, as well as chemicals, plus access to more than 200 top brands and professional advice from our experts. Our pledge is quality at attractive prices. That‘s why, four times a year, we offer promotional prices on selected products and applications in our Life Science Specials. Single Cell DNA AMPLIFICATION AND DNA PURIFICATION illustraTM Single Cell GenomiPhi With very small starting quantities of DNA, successful amplification depends on reagents of the very best quality. With illustraTM Single Cell GenomiPhi, high-quality reagents and an optional proprietary enzymatic purification step guarantee the removal of possible DNA contamination ahead of any reaction. The GE Healthcare production processes ensure that all kits are free of any detectable DNA contamination. NEW gDNA from one cell • Uniform amplification of genomic DNA from as little as a single cell • Sensitivity down to 1 femtogram of input DNA • Isothermal MDA method (Multiple Strand Displacement Amplification) using Phi29 DNA polymerase • Contamination with DNA is effectively eliminated • Amplifications can be completed in less than 3 hours with less than 15 minutes hands on time illustraTM Nap-5 columns The NAP-5 single-use columns are pre-packed with Sephadex™ G-25 DNA grade and allow DNA purification based on the gel filtration process. • For the rapid and efficient purification of DNA through desalting and buffer exchange • Also ideal for purifying oligonucleotides or very small fragments of DNA following PCR or labeling • Maximum sample volume 0.5 ml Sephadex G-50 Superfine Sephadex G-50 Superfine is used for desalting and buffer exchange of biomolecules > 30 kDa. • Suitable for purifying DNA through gel filtration • Superfine has the smallest particle size for greater efficiency with shorter diffusion distances Product Art. No. Quantity 29 108 107 25 Rkt 29 108 039 100 Rkt NAP-5 columns 17 085 302 50 pieces Sephadex G-50 Superfine 17 004 101 100 g Single Cell GenomiPhi Kit Single Cell GenomiPhi Kit Add Lysis Buffer Cell samples in PBS Add Neutralization Buffer Incubate 10 min at 65° C Add Reaction Master Mix Amplified gDNA Incubate 2h at 30° C Single Cell GenomiPhi DNA Amplification Workflow Please quote the Life Science special code: „LSS IV/2014“ with your order. Th. Geyer Life Science Special IV/2014 3 ANTIBIOTICS, ANTIBODIES AND MULTI-TEST SLIDES MP Biomedicals Antibiotics Antibiotic solutions and powders from MP Biomedicals offer your cell cultures effective protection against contamination from bacteria and fungi. They are also ideal for selecting specific cell clones. Product Composition: •1 670 049 (50 x PenicilllinStreptomycin): 5.000 IU/ml Penicillin, 5 mg/ml Streptomycin •1 670 249 (100 x PenicillinStreptomycin): 10.000 IU/ml Penicillin, 10 mg/ml Streptomycin •1 674 049 (100 x PenicillinStreptomycin-Amphotericin B): 10.000 IU/ml Penicillin, 10 mg/ml Streptomycin, 25 µg/ml Amphotericin B Art. No. Quantity Amphotericin B, > 800 µg/mg 195 043 1g 5g Blasticidin S Hydrochlorid 150 477 100 mg Geneticin Disulfat (G418) 158 782 1g 5g Penicillin-Streptomycin solution (50x) 1 670 049 12 x 100 ml Penicillin-Streptomycin solution (100x) 1 670 249 12 x 100 ml Penicillin- Streptomycin-Amphotericin B solution (100x) 1 674 049 12 x 100 ml Antibodies Antibodies for detection of GFP (green fluorescent protein): • Clone 3E6: recognizes the native form of GFP, GFP variants and GFP fusion proteins. Very specific and sensitive for immune precipitation, immunocytochemistry, ELISA and purification of GFP fusion proteins. • Clone 11E5: detects the denatured form of GFP, GFP variants and GFP fusion proteins. Ideally suited for Western Blot analyses as well as in the use of colorimetric and chemiluminescence-based methods. Monoclonal antibodies for the detection of actin and tubulin: • detection of all known actins and tubulins. Ideally suited for positive controls in Western Blot analyses and immunofluorescence staining. Product Art. No. Quantity 687 371 100 µg Anti-GFP, mouse, clone 11E5 687 381 100 µg Anti-Actin, mouse, clone C4 691 001 691 002 100 µl 200 µl α-Tubulin, mouse, clone DM1A 691 251 1 ml Anti-GFP, mouse, clone 3E6 Multi-test slides The multi-test slides made from high-quality glass are ideal for a variety of applications. Their PTFE coating makes them suitable for a wide variety of cell culture and histology applications, as well as for immunofluorescence staining and slide agglutination tests. • Self-drying surface • Resistant to conventional organic solvents and fixing solutions • Heat resistant, sterilisable Product Multitest slide, 10 well (1 Box = 100 pieces) 4 Th. Geyer Life Science Special IV/2014 Art. No. Quantity 60 418 05E 1 Box Please quote the Life Science special code: „LSS IV/2014“ with your order. MYCOPLASMA - PREVENTION, DETECTION AND ELIMINATION biowest offers you a complete product range to protect your cell culture laboratory against mycoplasma. It includes disinfectants, a PCR kit for the detection of mycoplasma DNA in your cells and antibiotics for the elimination of mycoplasma and other bacteria from your cell culture. Bioguard Effective protection against mycoplasma. For the effective and preventative disinfection of surfaces, incubators and water baths in your cell culture laboratory. Product Art. No. Quantity Bioguard S – Spray for the disinfection of surfaces D1010 1 000 ml Bioguard-A – Solution for the disinfection of incubator water baths (100x) D1020 100 ml Bioguard-B – Solution for the disinfection of laboratory water baths (500x) D1030 50 ml Mycokit Time-saving, „ready-to-use“ PCR detection kit for the detection of mycoplasma DNA in the supernatant of your cell culture. The primers detect the highly conserved 16S rRNA region, allowing the detection of all conventional types of mycoplasma. The Taq polymerase is already included in the kit. Included in each kit: • PCR reaction mix, 200 µl (incl. Taq polymerase) • Buffer solution, 1 ml • Template for positive control, 20 µl Product Mycokit detection NEW Art. No. Quantity K1000 20 Tests Nanomycopulitine® concentrates For protection against bacterial contaminations and their rapid and simple elimination from cell cultures. Effective against a broad spectrum of gram-positive and gram-negative bacteria. Product Nanomycopulitine® Concentrate 20x Art. No. Quantity L-X16 100 ml Mycoplasma are very small bacteria (0.2 - 2 µm) which have no cell wall. Because of their small size they can bypass conventional sterile filters and enter cell cultures, where they primarily grow on the cell surfaces. Contamination with mycoplasma very often remains undetected, however it can severely impair the metabolism of cultivated cells and lead to reduced proliferation rates or altered results. Please quote the Life Science special code: „LSS IV/2014“ with your order. Th. Geyer Life Science Special IV/2014 5 SYRINGE FILTERS Syringe filters, acrylic housing 13 mm LABSOLUTE® syringe filters with a filter membrane diameter of 13 mm are ideal for the preparation of small-volume samples in which a very small dead volume is particularly important. The effective filtration area is 0.6 cm2. The housing, made from blue (pore size: 0.22 µm) or yellow (pore size: 0.45 µm) acrylic, has a pressure resistance of up to 5 bar. The non-sterile filters are packed in quantities of 500 pieces in polypropylene pouches, while sterile filters are packaged in blisters in quantities of 50 pieces per box. Properties: • Membrane diameter 13 mm (surface 0.6 cm2) • Housing diameter 18 mm • Low content of extractable substances • Very low dead volume Product new Pore size Sterile Art. No. Quantity Celluloseacetate (CA) 0,22 µm unsterile 7 699 800 500 Celluloseacetate (CA) 0,45 µm unsterile 7 699 801 500 Celluloseacetate (CA) 0,22 µm sterile 7 699 802 50 Celluloseacetate (CA) 0,45 µm sterile 7 699 803 50 Syringe filters, acrylic housing 25 mm LABSOLUTE® syringe filters with a filter membrane diameter of 25 mm are ideal for the preparation of samples with a volume from 1.5 ml to 100 ml. The effective filtration area is 4.6 cm2. The stable acrylic housing can withstand pressures of up to 5 bar (pore size: 0.22 µm blue or pore size: 0.45 µm yellow), this means they allow particularly fast filtration. Membrane properties: The cellulose acetate membrane is hydrophilic and is therefore particularly ideal for the filtration of aqueous and alcoholcontaining samples. It also has particularly good resistance to solvents and higher temperatures, as well as a low protein binding capacity. The non-sterile filters are packed in quantities of 500 pieces in polypropylene pouches, while sterile filters are packaged in blisters in quantities of 50 pieces per box. Properties: • Membrane diameter 25 mm (surface 4.6 cm2) • Housing diameter 33 mm • Low content of extractable substances • Low dead volume Product Applications: Filtration of protein and enzymecontaining solutions with maximum protein recovery. Filtration and sterilisation of biological liquids and cell culture media. Clarification of aqueous and alcoholic solutions. 6 Th. Geyer Life Science Special IV/2014 Pore size Sterile Art. No. Quantity Celluloseacetate (CA) 0,22 µm unsterile 7 699 820 500 Celluloseacetate (CA) 0,45 µm unsterile 7 699 821 500 Celluloseacetate (CA) 0,22 µm sterile 7 699 822 50 Celluloseacetate (CA) 0,45 µm sterile 7 699 823 50 Please quote the Life Science special code: „LSS IV/2014“ with your order. Sprout® Mini CENTRIFUGE The compact Sprout® mini centrifuge is ideal for fast centrifugation, microfilter cell separations and HPLC samples. • Straightforward rotor changes without tools • For repeated centrifugation, simply open or close the lid • Max. speed: ~ 6,000 rpm, centrifugal force: max. 2,000 g • Dimensions: 153 x 128 x 104 mm STANDS FOR REACTION VESSELS AND CENTRIFUGE TUBES Scope of delivery 2 rotor attachments (for 6x 1.5 ml or 2 ml tubes and 16x 0.2 ml tubes), storage box, tube rack, adapter for 6x 0.4 ml or 0.5 ml tubes • Two-sided stands for 0.5 ml or 1.5 ml + 2 ml reaction tubes. • Autoclavable 5 x, 1 x natural, blue, green, pink, yellow. • Work2StoreTM – expandable and autoclavable cryobox. (1) • Two versions: For 1.5 ml and 2 ml tubes (133 x 133 x 53 mm) or can be used on both sides for 0.5 ml or 0.2 ml tubes (133 x 133 x 48 mm) (2) • Stands for individual 50 ml centrifugation 3) tubes. Autoclavable. 5 x, 1 x orange, blue, green, pink, yellow. (3) • Pop-UpTM rack – collapsable rock for 15 ml and/or 50 ml tubes in four versions. 2 x each. (4) Product Product Sprout Mini centrifuge Quantity Art. No. 1 6 254 604 Stands for 96x 0,5 ml o. 96x 1,5/ 2,0 ml, coloured sorted (1) Work2Store for 64x 1,5/2,0 ml (2) Work2Store Micro for 64x 0,5ml/ 96x 0,2ml (2) Stands for 50 ml vessels, coloured sorted (3) Pop-Up Rack for 21 x 15 ml + 12x 50 ml, blue (4) Pop-Up Rack for 21 x 15 ml + 12x 50 ml, pink (4) Pop-Up Rack for 45 x 15 ml, violet (4) Pop-Up Rack für 18 x 50 ml, green (4) Quantity Art. No. 5 9 193 982 1 9 194 001 1 9 194 009 5 6 238 934 2 9 193 998 2 9 193 997 2 6 254 577 2 6 254 578 2) 4) 1) Please quote the Life Science special code: „LSS IV/2014“ with your order. Th. Geyer Life Science Special IV/2014 7 BVC Fluid ASPIRATION SYSTEMS The BioChem VacuuCenter BVC control and professional models are used for the efficient and convenient aspiration of liquid supernatants: BVC control • Powerful and durable chemistry diaphragm pump for universal and economical use • Extremely compact, space-saving and quiet, making it the ideal tool in the workplace • Automatic, needs-based switching on and off • High user convenience thanks to suction force controlled electronically via a touch panel for powerful or gentle aspiration • Perfectly equipped autoclavable 4 l PP collecting bottle to reduce aerosol and foam build-up, with hydrophobic 0.2 µm protection filter (autoclavable quick-couplings available as accessory) • Incl. VHCpro hand controller, prepared for the connection of a second aspiration hand controller (optional) BVC professional • With all the advantages of the BVC control • Non-contact sensor for the electronic monitoring of the liquid level in the collection bottle • Disinfection routine for the suction tubing for drawing in disinfectant after the pump has been switched off • Self-closing quick couplings made from PVDF for the convenient and secure changing of bottles, for example with biohazards • For professional work and a perfect fit for required safety procedures Product Art. No. Quantity BVC control 9 882 227 1 BVC professional 9 882 229 1 Technical data BVC control/ professional Max. pumping speed at 50/60 Hz (m3/h) 0.7 / 0.85 Max. pumping speed at 50/60 Hz (cfm) 0.4 / 0.5 Ultimate vacuum (abs.) (mbar/torr) 150 / 112 Control range 150 - 850 mbar (adjustable) Collection bottle 4l polypropylene Dimensions (L x W x H) (mm) 408 x 194 x 500 Weight (kg) 7,3 Noise level at 50 Hz, typ. (dBA) 45 Accessories for a second handset Product 8 Th. Geyer Life Science Special IV/2014 Art. No. Quantity VacuuHandControl VHCpro 6 260 677 1 Expansion kit for second VHCpro (BVC control) 9 882 983 1 Quick coupling set, VHCpro-bottle 6 254 294 1 Quick coupling set, connection bottle-pump 6 254 295 1 Please quote the Life Science special code: „LSS IV/2014“ with your order. CB CO2 Incubators Cell and tissue cultures require the best culture conditions and reliable protection against contamination. Many conventional CO2 incubators try to satisfy these requirements through increasingly complex, time-consuming and costly technical solutions. BINDER, on the other hand, focuses on the most essential properties of CO2 incubation and, with a range of carefully thought out technical features, pursues the strategy of „Less is more!“ The benefits for you: • Optimised cell growth • Minimised risk of contamination • Easy cleaning, automatic sterilization process • Low maintenance costs With sterilisable sensor CB series The BINDER CO2 BC series incubator is the premium class among CO2 incubators. It is suitable for all sensitive incubation applications and ensures optimal growth. A BINDER CO2 incubator of the CB series is up to even complex cultivation experiments or individual environments thanks to its extensive collection of options and accessories. • ANTI.PLENUM interior room concept „Less is more“ • Hot air sterilisation at 180 °C, fixed-installation, sterilisable CO2 sensor • Unique BINDER technologies (patented air jacket system, controlled condensation, etc.) Product Art. No. Quantity CB 60 (53 Liter) 9 883 726 1 CB 160 (150 Liter) 9 883 727 1 CB 220 (210 Liter) 9 883 728 1 Please quote the Life Science special code: „LSS IV/2014“ with your order. Th. Geyer Life Science Special IV/2014 9 CELL AND TISSUE CULTURE FlaskS AND PLATES With the CELLSTAR® product line, Greiner Bio-One offers a broad spectrum of cell culture flasks, dishes and plates. Because of their functional design, particular material properties and special coatings, the vessels are used not only in standard cell culture but also in cancer research, virus diagnosis, genetic engineering and vaccine production. CELLSTAR® cell culture bottles The special design of the Greiner Bio-One cell culture flasks allows perfect access with the pipette. The patented membrane with a constant pore diameter of 0.2 µm is made from PET and is coated with hydrophobic PTFE. • The cell layer is easily accessible with a cell scraper • The neck of the high profile flask reduces the risk of wetting the inside of the cap with medium therefore providing additional protection against contamination • Graduation on two sides • Free from detectable DNases, RNases and human DNA • Free from detectable endotoxins, free from cytotoxic substances Filter Top cell culture bottles Improved cell adhesion due to physical surface treatment Product Art. No. Quantity T 25, max. 50 ml (pouch with 10 pieces) 7 608 503 3 x 200 T 75, max. 250 ml (pouch with 5 pieces) 6 305 116 3 x 120 T 175, max. 550 ml (pouch with 5 pieces) 6 305 115 3 x 50 T 175, max. 650 ml (pouch with 4 pieces) 7 613 798 3 x 40 CELLSTAR® Multiwell plates The Multiwell plates for adherent cells have a high transparency and low auto-fluorescence. They are compatible with automated systems. • Hydrophilic surfaces for improved cell adhesion • Alphanumeric well coding • Individually-packaged Product 10 Th. Geyer Life Science Special IV/2014 Art. No. Quantity 6 Well format 7 600 501 3 x 100 12 Well format 7 601 920 3 x 100 24 Well format 6 075 823 3 x 100 48 Well format 7 610 559 3 x 100 Please quote the Life Science special code: „LSS IV/2014“ with your order. Please quote the Life Science special code: „LSS IV/2014“ with your order. Technical modifications and mistakes reserved. All prices are quoted net of VAT. Our prevailing terms and conditions of sale and delivery apply. Deliveries over Euro 50 free ex domicile! (Does not include dry ice deliveries) Germany Tel.: 0800 4393784 Fax: 0800 8443937 [email protected] Denmark Tel.: +45 4630 0030 Fax: +45 4630 0069 [email protected] www.thgeyer.com Sweden Tel.: +46 8 6030200 Fax: +46 8 6030229 [email protected] Other countries Tel.: +49 7159 1637-823 Fax: +49 7159 18417 [email protected]
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