AquatiqPCR©

Advanced molecular diagnostics
©
AquatiqPCR
Molecular detection kits for waterborne pathogens
The AquatiqPCR© unique line of product is based on patented
chimeric DNA/RNA primer/probes technology and proprietary
computational platform; resulting in highly specific and
supersensitive detection, down to single-cell.
AquatiqPCR© is today's best choice for the detection of E-coli
pathogenic Cryptosporidium and Giardia in water samples.
AquatiqPCR© kits offer faster, highly sensitive, accurate and
pathogen specific results.
No Microscope
Needed
Sensitive
Specific
Fast
www.genaphora.com
Robust
Cost Effective
©
AquatiqPCR -E
Advanced molecular diagnostics
Molecular detection of E. coli in water
AquatiqPCR-E 30-004 (24 samples)
A singleplex assay for the detection of E. coli spp. in water
Advantages:
Sensitive (down to single CFU/sample)
Specific (detects only E. coli spp. and Shigella )
Fast - unequivocal results within 3hrs. (Instead of 24-48hrs.)
AquatiqPCR-E: Assay sensitivity
Detection of E. coli in drinking water
using Genaphora’s AquatiqPCR-E kit.
The L.O.D of AquatiqPCR-E is as low as 1-2
CFU of E. coli per tube and meets the
regulatory requirements. Culture
enrichment process ensures the detection
of living E. coli and Shigella.
ΔCp, AquatiqPCR E, E. coli in Drinking Water.
Direct DNA extraction vs 3 hours enrichment
25
20
15
10
5
0
0 DDW 0 spiking 2 E. coli
9 E. coli
86 E. coli 86 E. coli
2. E. coli
9 E. coli
Enrichment
Enrichment
Enrichment
AquatiqPCR-E: Assay specificity
NTC
NTC
Seratia narcescens
Proteus sp
Salmonella sp
Providencia stuartii
Enterobacter aerogenes
Enterobacter cloacae
Enerobacter sakazakii
Citrobacter koseri
Citrobacter freundii
Klebsiella oxytoca
Klebsiella pneumoniae
E.coli ATCC 25922
E.coli ATCC 35218
Shigella sonneei
AquatiqPCR-E was tested against several Coliforms
and other closely related bacteria to E. coli and
was found to be specific only to E. coli and Shigella
(a closely related pathogenic species which can be
separated only by discriminating test).
ΔCp, AquatiqPCR E, E. in different coliforms
18
16
14
12
10
8
6
4
2
0
Shigella flexnerri
Genaphora’s AquatiqPCR-E is specific for E.
coli and Shigella spp only.
Advanced molecular diagnostics
Genaphora is introducing a breakthrough in endpoint and real time qPCR diagnostics. Genaphora, has now developed groundbreaking, real
time, quantitative PCR (qPCR) and endpoint PCR diagnostic kits. Our proprietary computational biology and chimeric DNA/RNA primer and
probes technology for increased sensitivity and specificity have been tested and patented. Genaphora’s assays provide unprecedented
sensitivity - down to single copy detection. Genaphra has established a strategic partnership with Mekorot, Israel National Water Company.
Contact Us
4 Habarzel st. Tel-Aviv 69710, Israel Tel. +972-3-6492444 Fax. +972-3-6496664 [email protected] www.genaphora.com

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